

CUTANA™ CUT&Tag Kit
{"url":"https://www.epicypher.com/products/epigenetics-kits-and-reagents/cutana-cut-and-tag-kit","add_this":[{"service":"facebook","annotation":""},{"service":"email","annotation":""},{"service":"print","annotation":""},{"service":"twitter","annotation":""},{"service":"linkedin","annotation":""}],"gtin":"","id":"986","bulk_discount_rates":[],"can_purchase":true,"meta_description":"CUTANA™ CUT&Tag Kit includes all needed reagents to go from cells to purified sequence-ready DNA. ","category":["Antibodies/CUTANA™ CUT&Tag Antibodies","Epigenetics Kits and Reagents/CUTANA™ CUT&Tag Assays"],"AddThisServiceButtonMeta":"","main_image":{"data":"https://cdn11.bigcommerce.com/s-y9o92/images/stencil/{:size}/products/986/1117/Save_15_with_code_CTK15__15909.1678741803.png?c=2","alt":"CUTANA™ CUT&Tag Kit"},"add_to_wishlist_url":"/wishlist.php?action=add&product_id=986","shipping":{"calculated":true},"num_reviews":0,"weight":"0.01 LBS","custom_fields":[{"id":"1219","name":"Pack Size","value":"48 Reactions"}],"sku":"14-1102","description":"<div class=\"service_accordion product-droppdown\">\n <div class=\"container\">\n <div id=\"prodAccordion\">\n <div id=\"ProductDescription\" class=\"Block Panel current\">\n <h3 class=\"sub-title1\">Description</h3>\n <div\n class=\"ProductDescriptionContainer product-droppdown__section-description-specific\">\n <p>\n The CUTANA™ CUT&Tag Kit offers a comprehensive solution for\n ultra-sensitive mapping of histone post-translational modifications\n (PTMs). This kit uses an exclusive Direct-to-PCR strategy to go from\n cells to PCR amplified sequencing libraries in one tube, bypassing\n traditional library prep and minimizing sample loss. The protocol is\n also designed for compatibility with multi-channel pipetting for\n increased throughput and reproducibility. Positive (H3K27me3) and\n negative (IgG) control antibodies are paired with the SNAP-CUTANA™\n K-MetStat Panel of nucleosome spike-in controls (<strong\n >Figure 2</strong\n >) to continuously monitor workflows and guide troubleshooting.\n </p>\n <p>\n The recommended input for CUT&Tag is 100,000 native nuclei per\n reaction. Comparable data can be generated down to 10,000 nuclei,\n and the protocol is also validated for whole cells, cryopreserved\n samples, and lightly cross-linked nuclei or cells. CUT&Tag provides\n robust profiling for histone PTMs. For chromatin-associated proteins\n (e.g. transcription factors), CUTANA™ CUT&RUN is recommended\n (EpiCypher\n <a\n href=\"/products/epigenetics-reagents-and-assays/cutana-chic-cut-and-run-kit\"\n >14-1048</a\n >, EpiCypher\n <a\n href=\"/products/epigenetics-reagents-and-assays/cutana-cut-and-run-library-prep-kit\"\n >14-1001/14-1002</a\n >).\n </p>\n </div>\n </div>\n </div>\n <div id=\"prodAccordion\">\n <div id=\"ProductDescription\" class=\"Block Panel current\">\n <h3 class=\"sub-title1\">Validation Data</h3>\n <div\n class=\"ProductDescriptionContainer product-droppdown__section-description-specific\">\n <p>\n CUT&Tag was performed using the CUTANA™ CUT&Tag Kit starting with\n 100k K562 cells and 0.5 µg of either IgG (EpiCypher\n <a\n href=\"/products/nucleosomes/snap-cutana-spike-in-controls/cutana-rabbit-igg-cut-run-negative-control-antibody\"\n >13-0042</a\n >t), H3K27me3 (EpiCypher\n <a\n href=\"/products/antibodies/h3k27me3-antibody-snap-certified-for-cut-run-and-cut-and-tag\"\n >13-0055</a\n >t), H3K4me1 (EpiCypher\n <a\n href=\"/products/antibodies/cut-and-run-antibodies/cut-and-run-antibodies-histone-ptms/h3k4me1-antibody-snap-certified-for-cut-and-run-and-cut-and-tag\"\n >13-0057</a\n >), or H3K4me3 (EpiCypher\n <a\n href=\"/products/antibodies/snap-chip-certified-antibodies/histone-h3k4me3-antibody-snap-chip-certified-cutana-cut-run-compatible\"\n >13-0041</a\n >*) antibodies. Libraries were run on an Illumina NextSeq2000 with\n paired-end sequencing (2x50 bp). Sample sequencing depth was 6.3/4.7\n million reads (IgG Rep 1/Rep 2), 4.1/4.6 million reads (H3K27me3 Rep\n 1/Rep 2), 4.7/5.0 million reads (H3K4me1 Rep 1/Rep 2), and 5.0/4.5\n million reads (H3K4me3 Rep 1/Rep 2). Data were aligned to the hg19\n genome using Bowtie2. Data were filtered to remove duplicates,\n multi-aligned reads, and blacklist regions.\n </p>\n\n <p>\n *EpiCypher\n <a\n href=\"/products/antibodies/snap-chip-certified-antibodies/histone-h3k4me3-antibody-snap-chip-certified-cutana-cut-run-compatible\"\n >13-0041</a\n >\n does not currently meet our “SNAP-Certified in CUT&Tag” efficiency\n standards for robust profiling down to 10k nuclei, but is specific\n at 100k nuclei.\n </p>\n <section class=\"image-picker\">\n <div class=\"image-picker__left\">\n <div\n class=\"image-picker__main-content_active image-picker__main-content\">\n <div class=\"image-picker__header-content\">\n <button class=\"image-picker__left-arrow\">\n <svg\n class=\"image-picker__svg-left\"\n width=\"24\"\n height=\"24\"\n viewBox=\"0 0 24 24\">\n <path\n d=\"M16.67 0l2.83 2.829-9.339 9.175 9.339 9.167-2.83 2.829-12.17-11.996z\" />\n </svg>\n </button>\n <a\n href=\"/content/images/products/kits/14-1102-distribution-analysis.jpeg\"\n target=\"_new\"\n class=\"image-picker__main-image-link\"\n ><img\n alt=\"14-1102-distribution-analysis\"\n src=\"/content/images/products/kits/14-1102-distribution-analysis.jpeg\"\n class=\"image-picker__main-image\" />\n <span class=\"image-picker__main-image-caption\"\n >(Click to enlarge)</span\n ></a\n >\n <button class=\"image-picker__right-arrow\">\n <svg\n class=\"image-picker__svg-right\"\n width=\"24\"\n height=\"24\"\n viewBox=\"0 0 24 24\">\n <path\n d=\"M7.33 24l-2.83-2.829 9.339-9.175-9.339-9.167 2.83-2.829 12.17 11.996z\" />\n </svg>\n </button>\n </div>\n <p>\n <span class=\"image-picker__span-content\"\n ><strong\n >Figure 1: CUT&Tag DNA fragment size distribution\n analysis</strong\n ><br />\n CUT&Tag was performed as described above. Library DNA was\n analyzed by Agilent TapeStation<sup>®</sup>, which confirmed\n that mononucleosomes were predominantly enriched in CUT&Tag\n (peak between 300-400 bp). Peak between 500-700 bp\n represents dinucleosomes.\n </span>\n </p>\n </div>\n <div class=\"image-picker__main-content\">\n <div class=\"image-picker__header-content\">\n <button class=\"image-picker__left-arrow\">\n <svg\n class=\"image-picker__svg-left\"\n width=\"24\"\n height=\"24\"\n viewBox=\"0 0 24 24\">\n <path\n d=\"M16.67 0l2.83 2.829-9.339 9.175 9.339 9.167-2.83 2.829-12.17-11.996z\" />\n </svg>\n </button>\n <a\n href=\"/content/images/products/kits/14-1102-spike-in-controls.jpeg\"\n target=\"_new\"\n class=\"image-picker__main-image-link\"\n ><img\n alt=\"14-1102-spike-in-controls\"\n src=\"/content/images/products/kits/14-1102-spike-in-controls.jpeg\"\n class=\"image-picker__main-image\" />\n <span class=\"image-picker__main-image-caption\"\n >(Click to enlarge)</span\n ></a\n >\n <button class=\"image-picker__right-arrow\">\n <svg\n class=\"image-picker__svg-right\"\n width=\"24\"\n height=\"24\"\n viewBox=\"0 0 24 24\">\n <path\n d=\"M7.33 24l-2.83-2.829 9.339-9.175-9.339-9.167 2.83-2.829 12.17 11.996z\" />\n </svg>\n </button>\n </div>\n <p>\n <span class=\"image-picker__span-content\"\n ><strong\n >Figure 2: SNAP-CUTANA™ K-MetStat Spike-in\n controls</strong\n ><br />\n DNA-barcoded designer nucleosomes (dNucs) representing 16\n different K-methyl PTM states: mono-, di-, and\n tri-methylation at H3K4, H3K9, H3K27, H3K36, and H4K20, as\n well as unmodified control, were spiked into CUT&Tag samples\n prior to the addition of the control antibodies provided\n with the kit (IgG, H3K27me3). After sequencing, instances of\n each spike-in barcode recovered in the CUT&Tag reactions\n were counted and normalized from raw fastq files using the\n shell script and analysis excel sheet available on the\n spike-in product page (EpiCypher\n <a href=\"/products/nucleosomes/snap-cutana-k-metstat-panel\"\n >19-1002</a\n >). Barcodes for IgG (top; normalized to the sum of total\n reads) and H3K27me3 (bottom; normalized to on-target)\n antibodies provided with this kit are shown. The spike-ins\n confirmed optimal experimental conditions (H3K27me3 antibody\n specifically recovered the target dNuc, while IgG showed no\n preferential enrichment).\n </span>\n </p>\n </div>\n <div class=\"image-picker__main-content\">\n <div class=\"image-picker__header-content\">\n <button class=\"image-picker__left-arrow\">\n <svg\n class=\"image-picker__svg-left\"\n width=\"24\"\n height=\"24\"\n viewBox=\"0 0 24 24\">\n <path\n d=\"M16.67 0l2.83 2.829-9.339 9.175 9.339 9.167-2.83 2.829-12.17-11.996z\" />\n </svg>\n </button>\n <a\n href=\"/content/images/products/kits/14-1102-genome-wide-heatmaps.jpeg\"\n target=\"_new\"\n class=\"image-picker__main-image-link\">\n <img\n alt=\"14-1102-genome-wide-heatmaps\"\n src=\"/content/images/products/kits/14-1102-genome-wide-heatmaps.jpeg\"\n class=\"image-picker__main-image\" />\n <span class=\"image-picker__main-image-caption\"\n >(Click to enlarge)</span\n >\n </a>\n <button class=\"image-picker__right-arrow\">\n <svg\n class=\"image-picker__svg-right\"\n width=\"24\"\n height=\"24\"\n viewBox=\"0 0 24 24\">\n <path\n d=\"M7.33 24l-2.83-2.829 9.339-9.175-9.339-9.167 2.83-2.829 12.17 11.996z\" />\n </svg>\n </button>\n </div>\n <p>\n <span class=\"image-picker__span-content\">\n <strong>Figure 3: CUT&Tag genome-wide heatmaps</strong\n ><br />\n CUT&Tag was performed as described above. Heatmaps show two\n replicates (“Rep”) of IgG, H3K27me3, H3K4me1, and H3K4me3\n antibodies in aligned rows ranked by intensity (top to\n bottom) relative to the H3K4me3 Rep 1 reaction. High,\n medium, and low intensity are shown in red, yellow, and\n blue, respectively. Antibodies to histone PTMs showed\n expected enrichment patterns and high reproducibility.\n H3K4me3, a marker of active transcription localized to TSSs,\n shows oppositional enrichment to H3K27me3 (a marker of\n repressive chromatin), while H3K4me1 signal flanks TSSs. IgG\n shows low background enrichment.\n </span>\n </p>\n </div>\n <div class=\"image-picker__main-content\">\n <div class=\"image-picker__header-content\">\n <button class=\"image-picker__left-arrow\">\n <svg\n class=\"image-picker__svg-left\"\n width=\"24\"\n height=\"24\"\n viewBox=\"0 0 24 24\">\n <path\n d=\"M16.67 0l2.83 2.829-9.339 9.175 9.339 9.167-2.83 2.829-12.17-11.996z\" />\n </svg>\n </button>\n <a\n href=\"/content/images/products/kits/14-1102-gene-browser-tracks.jpeg\"\n target=\"_new\"\n class=\"image-picker__main-image-link\">\n <img\n alt=\"14-1102-gene-browser-tracks\"\n src=\"/content/images/products/kits/14-1102-gene-browser-tracks.jpeg\"\n class=\"image-picker__main-image\" />\n <span class=\"image-picker__main-image-caption\"\n >(Click to enlarge)</span\n >\n </a>\n <button class=\"image-picker__right-arrow\">\n <svg\n class=\"image-picker__svg-right\"\n width=\"24\"\n height=\"24\"\n viewBox=\"0 0 24 24\">\n <path\n d=\"M7.33 24l-2.83-2.829 9.339-9.175-9.339-9.167 2.83-2.829 12.17 11.996z\" />\n </svg>\n </button>\n </div>\n <p>\n <span class=\"image-picker__span-content\">\n <strong>Figure 4: Representative gene browser tracks</strong\n ><br />\n CUT&Tag was performed as described above. A representative\n 186 kb window at the LAMC3 gene is shown for two replicates\n (“Rep”) of IgG and H3K27me3 kit control antibodies.\n Representative tracks are also shown for two replicates of\n H3K4me1 and H3K4me3 antibodies. The CUT&Tag kit produced the\n expected genomic distribution for each target. Images were\n generated using the Integrative Genomics Viewer (IGV, Broad\n Institute).\n </span>\n </p>\n </div>\n </div>\n <aside class=\"image-picker__right\">\n <div class=\"image-picker__gallery\">\n <img\n alt=\"14-1102-distribution-analysis\"\n src=\"/content/images/products/kits/14-1102-distribution-analysis.jpeg\"\n width=\"200\"\n class=\"image-picker__side-image image-picker__side-image_active\"\n role=\"button\" />\n <img\n alt=\"14-1102-spike-in-controls\"\n src=\"/content/images/products/kits/14-1102-spike-in-controls.jpeg\"\n class=\"image-picker__side-image\"\n role=\"button\" />\n <img\n alt=\"14-1102-genome-wide-heatmaps\"\n src=\"/content/images/products/kits/14-1102-genome-wide-heatmaps.jpeg\"\n class=\"image-picker__side-image\"\n role=\"button\" />\n <img\n alt=\"14-1102-gene-browser-tracks\"\n src=\"/content/images/products/kits/14-1102-gene-browser-tracks.jpeg\"\n class=\"image-picker__side-image\"\n role=\"button\" />\n </div>\n </aside>\n </section>\n </div>\n </div>\n </div>\n <div id=\"prodAccordion\">\n <div id=\"ProductDescription\" class=\"Block Panel\">\n <h3 class=\"sub-title1\">Kit Contents</h3>\n <div\n class=\"ProductDescriptionContainer product-droppdown__section-description\">\n <table style=\"color: #525051\" id=\"specific-table\">\n <tr>\n <th\n style=\"\n padding: 0;\n text-align: left;\n color: #525051;\n text-transform: none;\n \">\n <u>Item</u>\n </th>\n <th style=\"padding: 0; text-align: left; color: #525051\">\n <u style=\"text-transform: none\">Cat. No.</u>\n </th>\n </tr>\n\n <tr>\n <td>pAG-Tn5</td>\n <td>\n <a\n href=\"/products/epigenetics-kits-and-reagents/cutana-pag-tn5-for-cut-and-tag\"\n >15-1017</a\n >\n </td>\n </tr>\n\n <tr>\n <td>H3K27me3 Positive Control Antibody</td>\n <td>\n <a\n href=\"/products/antibodies/h3k27me3-antibody-snap-certified-for-cut-run-and-cut-and-tag\"\n >13-0055</a\n >t\n </td>\n </tr>\n\n <tr>\n <td>Rabbit IgG Negative Control Antibody</td>\n <td>\n <a\n href=\"/products/nucleosomes/snap-cutana-spike-in-controls/cutana-rabbit-igg-cut-run-negative-control-antibody\"\n >13-0042</a\n >t\n </td>\n </tr>\n\n <tr>\n <td>Anti-Rabbit Secondary Antibody</td>\n <td>\n <a\n href=\"/products/antibodies/cutana-cut-tag-antibodies/anti-rabbit-secondary-antibody-for-cutana-cut-tag-workflows\"\n >13-0047</a\n >\n </td>\n </tr>\n\n <tr>\n <td>SNAP-CUTANA™ K-MetStat Panel</td>\n <td>\n <a href=\"/products/nucleosomes/snap-cutana-k-metstat-panel\"\n >19-1002</a\n >t\n </td>\n </tr>\n\n <tr>\n <td>ConA Beads</td>\n <td>\n <a\n href=\"/products/epigenetics-kits-and-reagents/cutana-chic-cut-run-assays/cutana-concanavalin-a-conjugated-paramagnetic-beads\"\n >21-1401</a\n >\n </td>\n </tr>\n\n <tr>\n <td>Non-Hot Start 2X PCR Master Mix</td>\n <td>\n <a\n href=\"/products/epigenetics-kits-and-reagents/cutana-cut-tag-assays/cutana-non-hot-start-2x-pcr-master-mix-for-cut-tag\"\n >15-1018</a\n >\n </td>\n </tr>\n\n <tr>\n <td>8-strip Tubes</td>\n <td>\n <a\n href=\"/products/epigenetics-kits-and-reagents/cutana-chic-cut-run-assays/cutana-cut-run-8-strip-0-2-ml-tubes\"\n >10-0009</a\n >t\n </td>\n </tr>\n\n <tr>\n <td>4.5 M NaCl</td>\n <td>21-1013</td>\n </tr>\n\n <tr>\n <td>0.5 M EDTA</td>\n <td>21-1014</td>\n </tr>\n\n <tr>\n <td>1 M MgCl<sub>2</sub></td>\n <td>21-1015</td>\n </tr>\n\n <tr>\n <td>TAPS Buffer</td>\n <td>21-1016</td>\n </tr>\n\n <tr>\n <td>SDS Release Buffer</td>\n <td>21-1017</td>\n </tr>\n\n <tr>\n <td>SDS Quench Buffer</td>\n <td>21-1018</td>\n </tr>\n\n <tr>\n <td>0.1X TE Buffer</td>\n <td>21-1019</td>\n </tr>\n\n <tr>\n <td>Pre-Wash Buffer</td>\n <td>21-1020</td>\n </tr>\n\n <tr>\n <td>Pre-Nuclear Extraction Buffer</td>\n <td>21-1021</td>\n </tr>\n\n <tr>\n <td>Bead Activation Buffer</td>\n <td>21-1022</td>\n </tr>\n\n <tr>\n <td>5% Digitonin</td>\n <td>21-2023</td>\n </tr>\n\n <tr>\n <td>1 M Spermidine</td>\n <td>21-1024</td>\n </tr>\n\n <tr>\n <td>SPRIselect reagent manufactured by Beckman Coulter, Inc</td>\n <td>21-1404</td>\n </tr>\n\n <tr>\n <td style=\"vertical-align: top\">Multiplexing Primers</td>\n <td>\n 14-1102 and 14-1103 each contain combinatorial dual indices for\n multiplexed sequencing of up to 48 reactions. Combine the kits\n to multiplex up to 96 reactions.\n </td>\n </tr>\n </table>\n </div>\n </div>\n </div>\n <div id=\"prodAccordion\">\n <div id=\"ProductDescription\" class=\"Block Panel\">\n <h3 class=\"sub-title1\">Recommended Accessory Products</h3>\n <div\n class=\"ProductDescriptionContainer product-droppdown__section-description\">\n <table style=\"color: #525051\" id=\"specific-table-second\">\n <tr>\n <th\n style=\"\n padding: 0;\n text-align: left;\n color: #525051;\n text-transform: none;\n \">\n <u>Item</u>\n </th>\n <th style=\"padding: 0; text-align: left; color: #525051\">\n <u style=\"text-transform: none\">Cat. No.</u>\n </th>\n </tr>\n\n <tr>\n <td id=\"specific-row\">\n Anti-Mouse Secondary Antibody for CUTANA™ CUT&Tag Workflows\n </td>\n <td>\n <a\n href=\"/products/epigenetics-reagents-and-assays/cutanac-cut-tag-assays/anti-mouse-secondary-antibody-for-cutana-cut-tag-workflows\"\n >13-0048</a\n >\n </td>\n </tr>\n\n <tr>\n <td>SNAP-CUTANA™ K-MetStat Panel</td>\n <td>\n <a href=\"/products/nucleosomes/snap-cutana-k-metstat-panel\"\n >19-1002</a\n >\n </td>\n </tr>\n\n <tr>\n <td id=\"specific-row\">CUT&Tag Antibodies</td>\n <td>\n <a href=\"/products/antibodies/cutana-cut-and-tag-antibodies\"\n >See the list</a\n >\n </td>\n </tr>\n\n <tr>\n <td id=\"specific-row\">Magnetic Separation Rack, 0.2 mL Tubes</td>\n <td>\n <a\n href=\"/products/epigenetics-reagents-and-assays/cutana-chic-cut-run-assays/magnetic-separation-rack-0-2-ml-tubes\"\n >10-0008</a\n >\n </td>\n </tr>\n\n <tr>\n <td id=\"specific-row\">Magnetic Separation Rack, 1.5 mL Tubes</td>\n <td>\n <a\n href=\"/products/epigenetics-reagents-and-assays/magnetic-separation-rack-1-5-ml-tubes\"\n >10-0012</a\n >\n </td>\n </tr>\n </table>\n </div>\n </div>\n </div>\n <div id=\"prodAccordion\">\n <div id=\"ProductDescription\" class=\"Block Panel\">\n <h3 class=\"sub-title1\">Technical Information</h3>\n <div\n class=\"ProductDescriptionContainer product-droppdown__section-description\">\n <div class=\"product-tech-info\">\n <div class=\"product-tech-info__line-item\">\n <div class=\"product-tech-info__line-item-left\">\n <b>Storage</b>\n </div>\n <div class=\"product-tech-info__line-item-right\">\n OPEN KIT IMMEDIATELY and store components at room temperature,\n 4°C, and -20°C as indicated (see Kit Manual). Stable for 6\n months upon date of receipt.\n </div>\n </div>\n <div class=\"product-tech-info__line-item\">\n <div class=\"product-tech-info__line-item-left\">\n <b>Instructions for Use</b>\n </div>\n <div class=\"product-tech-info__line-item-right\">\n See Kit Manual\n </div>\n </div>\n </div>\n </div>\n </div>\n </div>\n <div id=\"prodAccordion\">\n <div id=\"ProductDescription\" class=\"Block Panel\">\n <h3 class=\"sub-title1\">Additional Information</h3>\n <div\n class=\"ProductDescriptionContainer product-droppdown__section-description\">\n <p>\n Beckman Coulter, the stylized logo, and SPRIselect are trademarks or\n registered trademarks of Beckman Coulter, Inc. in the United States\n and other countries.\n </p>\n </div>\n </div>\n </div>\n <div id=\"prodAccordion\">\n <div id=\"ProductDescription\" class=\"Block Panel\">\n <h3 class=\"sub-title1\">Promo Code Details</h3>\n <div\n class=\"ProductDescriptionContainer product-droppdown__section-description\">\n <p>\n Promotional code CTK15 terms and conditions: Offer valid on CUT&Tag\n Kit purchases (cat# 14-1102 & #14-1103) made through 11:59pm ET on\n 09/30/23 on <a href=\"epicypher.com\">epicypher.com</a> and with\n participating distributors. Offer is only applicable to CUT&Tag Kit\n purchases, no other products are included in this promotion. Offer\n has no cash value and may not be altered, sold, or transferred. This\n promotion offer is subject to change, alteration, or termination by\n EpiCypher at its sole discretion at any time.\n </p>\n </div>\n </div>\n </div>\n <h3\n style=\"color: #4698cb; font-size: 24px; border-bottom: none\"\n class=\"custom-title-description\">\n Documents & Resources\n </h3>\n\n <div id=\"prodAccordion\">\n <div id=\"ProductDescription\" class=\"Block Panel\">\n <!-- <h3 style=\"color: #4698cb; margin-bottom: 0\">Current Lot</h3> -->\n <h3\n style=\"padding-bottom: 5px; margin-top: 1rem; padding-top: 0\"\n class=\"sub-title1\">\n Kit Version 1\n </h3>\n <div\n class=\"ProductDescriptionContainer product-droppdown__section-description\">\n <div class=\"product-documents\">\n <a\n href=\"/content/documents/tds/14-1102.pdf\"\n target=\"_new\"\n class=\"product-documents__link\">\n <svg\n version=\"1.1\"\n id=\"Layer_1\"\n xmlns=\"http://www.w3.org/2000/svg\"\n xmlns:xlink=\"http://www.w3.org/1999/xlink\"\n x=\"0px\"\n y=\"0px\"\n viewBox=\"0 0 228 240\"\n 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class=\"product-documents__svg-pdf\"\n d=\"M69.92,125.68h18.91c5.29,0,9.84,0.97,13.66,2.9c3.82,1.93,6.74,4.72,8.76,8.35\n c2.02,3.63,3.04,7.98,3.04,13.04c0,5.06-1,9.42-3,13.08c-2,3.66-4.91,6.45-8.73,8.38c-3.82,1.93-8.4,2.9-13.73,2.9H69.92V125.68z\n M88.07,165.63c10.35,0,15.52-5.22,15.52-15.66c0-10.4-5.17-15.59-15.52-15.59h-7.38v31.26H88.07z\" />\n <path\n class=\"product-documents__svg-pdf\"\n d=\"M122.57,125.68h32.84v8.49h-22.22v11.18h20.84v8.49h-20.84v20.49h-10.63V125.68z\" />\n </g>\n </svg>\n <span class=\"product-documents__info\"\n >Technical Datasheet (14-1102)</span\n >\n </a>\n </div>\n <div class=\"product-documents\">\n <a\n href=\"/content/documents/tds/14-1103.pdf\"\n target=\"_new\"\n class=\"product-documents__link\">\n <svg\n version=\"1.1\"\n id=\"Layer_1\"\n xmlns=\"http://www.w3.org/2000/svg\"\n xmlns:xlink=\"http://www.w3.org/1999/xlink\"\n x=\"0px\"\n y=\"0px\"\n viewBox=\"0 0 228 240\"\n style=\"enable-background: new 0 0 228 240\"\n xml:space=\"preserve\"\n 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d=\"M69.92,125.68h18.91c5.29,0,9.84,0.97,13.66,2.9c3.82,1.93,6.74,4.72,8.76,8.35\n c2.02,3.63,3.04,7.98,3.04,13.04c0,5.06-1,9.42-3,13.08c-2,3.66-4.91,6.45-8.73,8.38c-3.82,1.93-8.4,2.9-13.73,2.9H69.92V125.68z\n M88.07,165.63c10.35,0,15.52-5.22,15.52-15.66c0-10.4-5.17-15.59-15.52-15.59h-7.38v31.26H88.07z\" />\n <path\n class=\"product-documents__svg-pdf\"\n d=\"M122.57,125.68h32.84v8.49h-22.22v11.18h20.84v8.49h-20.84v20.49h-10.63V125.68z\" />\n </g>\n </svg>\n <span class=\"product-documents__info\"\n >Technical Datasheet (14-1103)</span\n >\n </a>\n </div>\n <div class=\"product-documents\">\n <a\n href=\"/content/documents/manuals/14-1102-cut-tag-kit-manual-v1.pdf\"\n target=\"_new\"\n class=\"product-documents__link\">\n <svg\n version=\"1.1\"\n id=\"Layer_1\"\n xmlns=\"http://www.w3.org/2000/svg\"\n xmlns:xlink=\"http://www.w3.org/1999/xlink\"\n x=\"0px\"\n y=\"0px\"\n viewBox=\"0 0 228 240\"\n style=\"enable-background: new 0 0 228 240\"\n xml:space=\"preserve\"\n class=\"product-documents__icon\"\n alt=\"16-0002 Datasheet\">\n <g>\n <path\n class=\"product-documents__svg-pdf\"\n d=\"M191.92,68.77l-47.69-47.69c-1.33-1.33-3.12-2.08-5.01-2.08H45.09C41.17,19,38,22.17,38,26.09v184.36\n c0,3.92,3.17,7.09,7.09,7.09h141.82c3.92,0,7.09-3.17,7.09-7.09V73.8C194,71.92,193.25,70.1,191.92,68.77z M177.65,77.06h-41.7\n v-41.7L177.65,77.06z M178.05,201.59H53.95V34.95h66.92v47.86c0,5.14,4.17,9.31,9.31,9.31h47.86V201.59z\" />\n </g>\n <rect\n x=\"20\"\n y=\"112\"\n class=\"product-documents__svg-background\"\n width=\"146\"\n height=\"76\" />\n <g>\n <path\n class=\"product-documents__svg-pdf\"\n d=\"M23.83,125.68h22.36c5.29,0,9.41,1.33,12.35,4c2.94,2.67,4.42,6.39,4.42,11.18c0,4.78-1.47,8.51-4.42,11.18\n c-2.94,2.67-7.06,4-12.35,4H34.59v18.29H23.83V125.68z M44.81,147.9c5.38,0,8.07-2.32,8.07-6.97c0-2.39-0.67-4.16-2-5.31\n c-1.33-1.15-3.36-1.73-6.07-1.73H34.59v14.01H44.81z\" />\n <path\n class=\"product-documents__svg-pdf\"\n d=\"M69.92,125.68h18.91c5.29,0,9.84,0.97,13.66,2.9c3.82,1.93,6.74,4.72,8.76,8.35\n c2.02,3.63,3.04,7.98,3.04,13.04c0,5.06-1,9.42-3,13.08c-2,3.66-4.91,6.45-8.73,8.38c-3.82,1.93-8.4,2.9-13.73,2.9H69.92V125.68z\n M88.07,165.63c10.35,0,15.52-5.22,15.52-15.66c0-10.4-5.17-15.59-15.52-15.59h-7.38v31.26H88.07z\" />\n <path\n class=\"product-documents__svg-pdf\"\n d=\"M122.57,125.68h32.84v8.49h-22.22v11.18h20.84v8.49h-20.84v20.49h-10.63V125.68z\" />\n </g>\n </svg>\n <span class=\"product-documents__info\">CUT&Tag Kit Manual</span>\n </a>\n </div>\n <div class=\"product-documents\">\n <a\n href=\"/content/documents/manuals/14-1102-cut-tag-quick-card.pdf\"\n target=\"_blank\"\n class=\"product-documents__link\">\n <svg\n version=\"1.1\"\n id=\"Layer_1\"\n xmlns=\"http://www.w3.org/2000/svg\"\n xmlns:xlink=\"http://www.w3.org/1999/xlink\"\n x=\"0px\"\n y=\"0px\"\n viewBox=\"0 0 228 240\"\n style=\"enable-background: new 0 0 228 240\"\n xml:space=\"preserve\"\n class=\"product-documents__icon\"\n alt=\"16-0002 Datasheet\">\n <g>\n <path\n class=\"product-documents__svg-pdf\"\n d=\"M191.92,68.77l-47.69-47.69c-1.33-1.33-3.12-2.08-5.01-2.08H45.09C41.17,19,38,22.17,38,26.09v184.36\n c0,3.92,3.17,7.09,7.09,7.09h141.82c3.92,0,7.09-3.17,7.09-7.09V73.8C194,71.92,193.25,70.1,191.92,68.77z M177.65,77.06h-41.7\n v-41.7L177.65,77.06z M178.05,201.59H53.95V34.95h66.92v47.86c0,5.14,4.17,9.31,9.31,9.31h47.86V201.59z\" />\n </g>\n <rect\n x=\"20\"\n y=\"112\"\n class=\"product-documents__svg-background\"\n width=\"146\"\n height=\"76\" />\n <g>\n <path\n class=\"product-documents__svg-pdf\"\n d=\"M23.83,125.68h22.36c5.29,0,9.41,1.33,12.35,4c2.94,2.67,4.42,6.39,4.42,11.18c0,4.78-1.47,8.51-4.42,11.18\n c-2.94,2.67-7.06,4-12.35,4H34.59v18.29H23.83V125.68z M44.81,147.9c5.38,0,8.07-2.32,8.07-6.97c0-2.39-0.67-4.16-2-5.31\n c-1.33-1.15-3.36-1.73-6.07-1.73H34.59v14.01H44.81z\" />\n <path\n class=\"product-documents__svg-pdf\"\n d=\"M69.92,125.68h18.91c5.29,0,9.84,0.97,13.66,2.9c3.82,1.93,6.74,4.72,8.76,8.35\n c2.02,3.63,3.04,7.98,3.04,13.04c0,5.06-1,9.42-3,13.08c-2,3.66-4.91,6.45-8.73,8.38c-3.82,1.93-8.4,2.9-13.73,2.9H69.92V125.68z\n M88.07,165.63c10.35,0,15.52-5.22,15.52-15.66c0-10.4-5.17-15.59-15.52-15.59h-7.38v31.26H88.07z\" />\n <path\n class=\"product-documents__svg-pdf\"\n d=\"M122.57,125.68h32.84v8.49h-22.22v11.18h20.84v8.49h-20.84v20.49h-10.63V125.68z\" />\n </g>\n </svg>\n <span class=\"product-documents__info\">\n CUT&Tag Kit Quick Card</span\n >\n </a>\n </div>\n <div class=\"product-documents\">\n <a\n href=\"/content/documents/SNAP-CUTANA_K-MetStat_Panel_ShellScript.sh\"\n target=\"_blank\"\n class=\"product-documents__link\">\n <svg\n version=\"1.1\"\n id=\"Layer_1\"\n xmlns=\"http://www.w3.org/2000/svg\"\n xmlns:xlink=\"http://www.w3.org/1999/xlink\"\n x=\"0px\"\n y=\"0px\"\n viewBox=\"0 0 228 240\"\n style=\"enable-background: new 0 0 228 240\"\n xml:space=\"preserve\"\n 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target=\"_blank\"\n class=\"product-documents__link\">\n <svg\n version=\"1.1\"\n id=\"Layer_1\"\n xmlns=\"http://www.w3.org/2000/svg\"\n xmlns:xlink=\"http://www.w3.org/1999/xlink\"\n x=\"0px\"\n y=\"0px\"\n viewBox=\"0 0 228 240\"\n style=\"enable-background: new 0 0 228 240\"\n xml:space=\"preserve\"\n class=\"product-documents__icon\">\n <g>\n <path\n class=\"product-documents__svg-excel\"\n d=\"M193.17,69.88l-46.83-46.83c-1.31-1.31-3.07-2.05-4.92-2.05H48.96C45.11,21,42,24.11,42,27.96v181.07\n c0,3.85,3.11,6.96,6.96,6.96h139.29c3.85,0,6.96-3.11,6.96-6.96V74.82C195.21,72.97,194.47,71.19,193.17,69.88L193.17,69.88z\n M179.15,78.02h-40.96V37.06L179.15,78.02z M179.54,200.33H57.67V36.67h65.73v47.01c0,5.05,4.09,9.14,9.14,9.14h47.01V200.33z\n M119.06,133.32l-13.45-22.29c-0.48-0.78-2.24-1.24-2.24-1.24h-8.33c-0.5,0-0.98,0.13-1.39,0.41c-1.21,0.76-1.58,2.36-0.8,3.6\n l17.85,28.28l-18.08,28.8c-0.76,1.22-0.39,2.83,0.84,3.6c0.41,0.26,0.89,0.39,1.37,0.39h7.48c0,0,1.74-0.26,2.22-1.02l13.65-22.09\n 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Description
The CUTANA™ CUT&Tag Kit offers a comprehensive solution for ultra-sensitive mapping of histone post-translational modifications (PTMs). This kit uses an exclusive Direct-to-PCR strategy to go from cells to PCR amplified sequencing libraries in one tube, bypassing traditional library prep and minimizing sample loss. The protocol is also designed for compatibility with multi-channel pipetting for increased throughput and reproducibility. Positive (H3K27me3) and negative (IgG) control antibodies are paired with the SNAP-CUTANA™ K-MetStat Panel of nucleosome spike-in controls (Figure 2) to continuously monitor workflows and guide troubleshooting.
The recommended input for CUT&Tag is 100,000 native nuclei per reaction. Comparable data can be generated down to 10,000 nuclei, and the protocol is also validated for whole cells, cryopreserved samples, and lightly cross-linked nuclei or cells. CUT&Tag provides robust profiling for histone PTMs. For chromatin-associated proteins (e.g. transcription factors), CUTANA™ CUT&RUN is recommended (EpiCypher 14-1048, EpiCypher 14-1001/14-1002).
Validation Data
CUT&Tag was performed using the CUTANA™ CUT&Tag Kit starting with 100k K562 cells and 0.5 µg of either IgG (EpiCypher 13-0042t), H3K27me3 (EpiCypher 13-0055t), H3K4me1 (EpiCypher 13-0057), or H3K4me3 (EpiCypher 13-0041*) antibodies. Libraries were run on an Illumina NextSeq2000 with paired-end sequencing (2x50 bp). Sample sequencing depth was 6.3/4.7 million reads (IgG Rep 1/Rep 2), 4.1/4.6 million reads (H3K27me3 Rep 1/Rep 2), 4.7/5.0 million reads (H3K4me1 Rep 1/Rep 2), and 5.0/4.5 million reads (H3K4me3 Rep 1/Rep 2). Data were aligned to the hg19 genome using Bowtie2. Data were filtered to remove duplicates, multi-aligned reads, and blacklist regions.
*EpiCypher 13-0041 does not currently meet our “SNAP-Certified in CUT&Tag” efficiency standards for robust profiling down to 10k nuclei, but is specific at 100k nuclei.
Figure 1: CUT&Tag DNA fragment size distribution
analysis
CUT&Tag was performed as described above. Library DNA was
analyzed by Agilent TapeStation®, which confirmed
that mononucleosomes were predominantly enriched in CUT&Tag
(peak between 300-400 bp). Peak between 500-700 bp
represents dinucleosomes.
Figure 2: SNAP-CUTANA™ K-MetStat Spike-in
controls
DNA-barcoded designer nucleosomes (dNucs) representing 16
different K-methyl PTM states: mono-, di-, and
tri-methylation at H3K4, H3K9, H3K27, H3K36, and H4K20, as
well as unmodified control, were spiked into CUT&Tag samples
prior to the addition of the control antibodies provided
with the kit (IgG, H3K27me3). After sequencing, instances of
each spike-in barcode recovered in the CUT&Tag reactions
were counted and normalized from raw fastq files using the
shell script and analysis excel sheet available on the
spike-in product page (EpiCypher
19-1002). Barcodes for IgG (top; normalized to the sum of total
reads) and H3K27me3 (bottom; normalized to on-target)
antibodies provided with this kit are shown. The spike-ins
confirmed optimal experimental conditions (H3K27me3 antibody
specifically recovered the target dNuc, while IgG showed no
preferential enrichment).
Figure 3: CUT&Tag genome-wide heatmaps
CUT&Tag was performed as described above. Heatmaps show two
replicates (“Rep”) of IgG, H3K27me3, H3K4me1, and H3K4me3
antibodies in aligned rows ranked by intensity (top to
bottom) relative to the H3K4me3 Rep 1 reaction. High,
medium, and low intensity are shown in red, yellow, and
blue, respectively. Antibodies to histone PTMs showed
expected enrichment patterns and high reproducibility.
H3K4me3, a marker of active transcription localized to TSSs,
shows oppositional enrichment to H3K27me3 (a marker of
repressive chromatin), while H3K4me1 signal flanks TSSs. IgG
shows low background enrichment.
Figure 4: Representative gene browser tracks
CUT&Tag was performed as described above. A representative
186 kb window at the LAMC3 gene is shown for two replicates
(“Rep”) of IgG and H3K27me3 kit control antibodies.
Representative tracks are also shown for two replicates of
H3K4me1 and H3K4me3 antibodies. The CUT&Tag kit produced the
expected genomic distribution for each target. Images were
generated using the Integrative Genomics Viewer (IGV, Broad
Institute).
Kit Contents
Item | Cat. No. |
---|---|
pAG-Tn5 | 15-1017 |
H3K27me3 Positive Control Antibody | 13-0055t |
Rabbit IgG Negative Control Antibody | 13-0042t |
Anti-Rabbit Secondary Antibody | 13-0047 |
SNAP-CUTANA™ K-MetStat Panel | 19-1002t |
ConA Beads | 21-1401 |
Non-Hot Start 2X PCR Master Mix | 15-1018 |
8-strip Tubes | 10-0009t |
4.5 M NaCl | 21-1013 |
0.5 M EDTA | 21-1014 |
1 M MgCl2 | 21-1015 |
TAPS Buffer | 21-1016 |
SDS Release Buffer | 21-1017 |
SDS Quench Buffer | 21-1018 |
0.1X TE Buffer | 21-1019 |
Pre-Wash Buffer | 21-1020 |
Pre-Nuclear Extraction Buffer | 21-1021 |
Bead Activation Buffer | 21-1022 |
5% Digitonin | 21-2023 |
1 M Spermidine | 21-1024 |
SPRIselect reagent manufactured by Beckman Coulter, Inc | 21-1404 |
Multiplexing Primers | 14-1102 and 14-1103 each contain combinatorial dual indices for multiplexed sequencing of up to 48 reactions. Combine the kits to multiplex up to 96 reactions. |
Recommended Accessory Products
Item | Cat. No. |
---|---|
Anti-Mouse Secondary Antibody for CUTANA™ CUT&Tag Workflows | 13-0048 |
SNAP-CUTANA™ K-MetStat Panel | 19-1002 |
CUT&Tag Antibodies | See the list |
Magnetic Separation Rack, 0.2 mL Tubes | 10-0008 |
Magnetic Separation Rack, 1.5 mL Tubes | 10-0012 |
Technical Information
Additional Information
Beckman Coulter, the stylized logo, and SPRIselect are trademarks or registered trademarks of Beckman Coulter, Inc. in the United States and other countries.
Promo Code Details
Promotional code CTK15 terms and conditions: Offer valid on CUT&Tag Kit purchases (cat# 14-1102 & #14-1103) made through 11:59pm ET on 09/30/23 on epicypher.com and with participating distributors. Offer is only applicable to CUT&Tag Kit purchases, no other products are included in this promotion. Offer has no cash value and may not be altered, sold, or transferred. This promotion offer is subject to change, alteration, or termination by EpiCypher at its sole discretion at any time.