Ultra-sensitive, low-input chromatin mapping assays
CUTANA™ ChIC / CUT&Tag assays build on recent advancements in immunotethering technology to deliver a highly sensitive and cost-effective workflow for epigenomic mapping. There are numerous advantages to CUT&Tag over ChIP-seq, the standard chromatin profiling assay:
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CUTANA™ CUT&Tag provides impressive gains compared to
the leading assay for chromatin and histone PTM profiling
How does CUT&Tag work?
Cleavage Under Targets and Tagmentation (CUT&Tag) is a novel immunotethering assay, based on the technology used in Chromatin ImmunoCleavage (ChIC) and Cleavage Under Targets and Release Using Nuclease (CUT&RUN) methods.
In CUT&Tag, a fusion of protein A, protein G, and Tn5 transposase is used to catalyze simultaneous cleavage and sequencing adapter ligation at antibody-bound chromatin. The use of Tn5 in CUT&Tag is a key advance, as this step eliminates the need for chromatin fragmentation as well as expensive library preparation.
The result is a highly cost- and time-efficient NGS assay, with improved signal : noise and genomic coverage vs. ChIP-seq.
Quality data with lower input and sequencing depth requirements
CUTANA™ CUT&Tag generates similar results at a fraction of the cell input and sequencing depth used in ChIP-seq. A representative 300 kb region at the LAMC3 gene is shown for CUT&Tag (orange), CUT&RUN (green) and ChIP-seq (blue) data generated using H3K4me3 and H3K27me3 antibodies (EpiCypher 13-0041 and 13-0030, respectively). Rabbit IgG Negative Control Antibody (EpiCypher 13-0042) and ChIP Input control are shown for comparison (control tracks are scaled to the track with the highest signal in each approach).
Go from cells to sequencing-ready DNA in < 2 days
Get started with CUTANA™ CUT&Tag assays
CUTANA™ pAG-Tn5 for ChIC / CUT&Tag
Plan your next CUT&Tag experiment! pAG-Tn5 is the essential enzyme for CUT&Tag assays. Available in 50 or 250 reaction sizes.
The CUTANA™ CUT&Tag Protocol
In addition to pAG-Tn5, EpiCypher offers many supporting products to help you get started with CUT&Tag assays, such as antibodies, experimental controls, magnetic beads, and more!
We have developed a streamlined, user-friendly CUT&Tag protocol, validated for:
- Histone PTMs and select validated targets
- Low cell inputs (down to 1,000 cells, without adaptions to protocol)
- Reduced sequencing depths (3-5 million reads / sample)
- High-throughput sample handling
The CUT&Tag protocol also contains a thorough FAQ section, in which we address the most common questions about the CUT&Tag assay. For more information on our protocol and how to set up your own assay, read our blog and our CUTANA CUT&Tag brochure.
Kaya-Okur et al. CUT&Tag for efficient epigenomic profiling of small samples and single cells. Nat. Comm. 10, 1930 (2019). (PMID: 31036827)
This paper introduces CUT&Tag methodology, as developed by the Henikoff laboratory at the Fred Hutchinson Cancer Research Center. It describes how the Tn5 fusion proteins works, demonstrates the application of CUT&Tag for histone PTM and chromatin factor profiling, and reveals major advantages of this new technology. Specifically, they use CUT&Tag to generate high-quality histone PTM profiles from single cells, a major advancement for the epigenetics field.
Kaya-Okur et al. Efficient low-cost chromatin profiling with CUT&Tag. Nat. Protoc. 15, 3264-3283 (2020). (PMID: 32913232)
Here, the inventors of CUT&Tag provide a highly detailed description of their optimized CUT&Tag protocol, citing EpiCypher’s pAG-Tn5 as the suggested commercial vendor of the enzyme. The protocol highlights critical steps, includes a troubleshooting FAQ table, and shows example results.
Henikoff et al. Efficient transcription-coupled chromatin accessibility mapping in situ. bioRxiv (2020). (doi: 10.1101/2020.04.15.043083)
A new preprint from the inventors of CUT&RUN / CUT&Tag technology leveraged EpiCypher’s pAG-Tn5 and CUT&RUN compatible histone PTM antibodies to develop a modified version of CUT&Tag for chromatin accessibility mapping. Importantly, this new method generates robust, high-quality profiles using only 3-5M reads/samples (vs. 20-30M reads for standard ATAC-seq).