

CUTANA™ Rabbit IgG CUT&RUN Negative Control Antibody
{"url":"https://www.epicypher.com/products/nucleosomes/snap-cutana-spike-in-controls/cutana-rabbit-igg-cut-run-negative-control-antibody","add_this":[{"service":"facebook","annotation":""},{"service":"email","annotation":""},{"service":"print","annotation":""},{"service":"twitter","annotation":""},{"service":"linkedin","annotation":""}],"gtin":null,"id":"758","bulk_discount_rates":[],"can_purchase":true,"meta_description":"CUTANA™ Rabbit IgG CUT&RUN Negative Control Antibody for ChIC/CUT&RUN Assays","category":["Nucleosomes/SNAP-CUTANA™ Spike-in Controls","Antibodies/CUTANA™ CUT&RUN Antibodies","Epigenetics Kits and Reagents","Epigenetics Kits and Reagents/CUTANA™ ChIC / CUT&RUN Assays","Epigenetics Kits and Reagents/CUTANA™ CUT&Tag Assays"],"AddThisServiceButtonMeta":"","main_image":{"data":"https://cdn11.bigcommerce.com/s-y9o92/images/stencil/{:size}/products/758/739/1579731877.1280.1280__61771.1592491196.png?c=2","alt":"CUTANA™ Rabbit IgG CUT&RUN Negative Control Antibody"},"add_to_wishlist_url":"/wishlist.php?action=add&product_id=758","shipping":{"calculated":true},"num_reviews":0,"weight":"0.01 LBS","custom_fields":[{"id":"1148","name":"Pack Size","value":"100 µg"},{"id":"1149","name":"Internal Comment","value":"bulk stocks from Thermo in cold room"}],"sku":"13-0042","description":"<div class=\"product-general-info\">\n <ul class=\"product-general-info__list-left\">\n <li class=\"product-general-info__list-item\">\n <strong>Type: </strong>Polyclonal\n </li>\n <li class=\"product-general-info__list-item\">\n <strong>Host: </strong>Rabbit\n </li>\n <li class=\"product-general-info__list-item\">\n <strong>Applications: </strong>CUT&RUN\n </li>\n </ul>\n <ul class=\"product-general-info__list-right\">\n <li class=\"product-general-info__list-item\">\n <strong>Reactivity: </strong>Negative Control\n </li>\n <li class=\"product-general-info__list-item\">\n <strong>Format: </strong>Affinity Purified IgG\n </li>\n <li class=\"product-general-info__list-item\">\n <strong>Target Size: </strong>N/A\n </li>\n </ul>\n <ul class=\"product-general-info__list-right\">\n <li class=\"product-general-info__list-item\">\n <a href=\"#bioz\">\n <div\n id=\"w-s-3835-13-0042\"\n style=\"\n width: max-content;\n height: 58px;\n position: relative;\n overflow-y: hidden;\n \"></div>\n <div id=\"bioz-w-pb-13-0042-div\">\n <a\n id=\"bioz-w-pb-13-0042\"\n style=\"font-size: 12px; color: transparent\"\n href=\"https://www.bioz.com/\"\n target=\"_blank\">\n <img\n src=\"https://cdn.bioz.com/assets/favicon.png\"\n style=\"\n width: 11px;\n height: 11px;\n vertical-align: baseline;\n padding-bottom: 0px;\n margin-left: 0px;\n margin-bottom: 0px;\n float: none;\n display: none;\n \" />\n </a></div\n ></a>\n </li>\n </ul>\n</div>\n<div class=\"service_accordion product-droppdown\">\n <div class=\"container\">\n <div id=\"prodAccordion\">\n <div id=\"ProductDescription\" class=\"Block Panel current\">\n <h3 class=\"sub-title1\">Description</h3>\n <div\n class=\"ProductDescriptionContainer product-droppdown__section-description-specific\">\n <p>\n This Rabbit IgG Antibody can be used to control for non-specific\n background signal in CUT&RUN and other applications. Pair with the\n H3K4me3 positive control antibody (EpiCypher\n <a\n href=\"https://www.epicypher.com/products/antibodies/snap-chip-certified-antibodies/histone-h3k4me3-antibody-snap-chip-certified-cutana-cut-run-compatible\"\n >13-0041</a\n >) for a well-controlled experiment.\n </p>\n </div>\n </div>\n </div>\n <div id=\"prodAccordion\">\n <div id=\"ProductDescription\" class=\"Block Panel current\">\n <h3 class=\"sub-title1\">Validation Data</h3>\n <div\n class=\"ProductDescriptionContainer product-droppdown__section-description-specific\">\n <section class=\"image-picker\">\n <div class=\"image-picker__left\">\n <div\n class=\"image-picker__main-content_active image-picker__main-content\">\n <div class=\"image-picker__header-content\">\n <button class=\"image-picker__left-arrow\">\n <svg\n class=\"image-picker__svg-left\"\n width=\"24\"\n height=\"24\"\n viewBox=\"0 0 24 24\">\n <path\n d=\"M16.67 0l2.83 2.829-9.339 9.175 9.339 9.167-2.83 2.829-12.17-11.996z\" />\n </svg>\n </button>\n <a\n href=\"/content/images/products/antibodies/13-0042_heatmap.jpg\"\n target=\"_new\"\n class=\"image-picker__main-image-link\"\n ><img\n loading=\"lazy\"\n alt=\"13-0042 Heat Map\"\n src=\"/content/images/products/antibodies/13-0042_heatmap.jpg\"\n class=\"image-picker__main-image\" />\n <span class=\"image-picker__main-image-caption\"\n >(Click to enlarge)</span\n ></a\n >\n <button class=\"image-picker__right-arrow\">\n <svg\n class=\"image-picker__svg-right\"\n width=\"24\"\n height=\"24\"\n viewBox=\"0 0 24 24\">\n <path\n d=\"M7.33 24l-2.83-2.829 9.339-9.175-9.339-9.167 2.83-2.829 12.17 11.996z\" />\n </svg>\n </button>\n </div>\n <p>\n <span class=\"image-picker__span-content\"\n ><strong>Figure 1: CUT&RUN genome wide enrichment</strong\n ><br />\n CUT&RUN data was generated using the EpiCypher CUTANA™\n CUT&RUN\n <a href=\"https://www.epicypher.com/resources/protocols\"\n >protocol</a\n >. Sequence reads were aligned to 18,793 annotated\n transcription start sites (TSSs ± 2 kbp). Signal enrichment\n was sorted from highest to lowest (top to bottom) relative\n to H3K4me3 CUT&RUN positive control antibody (EpiCypher\n <a\n href=\"https://www.epicypher.com/products/antibodies/snap-chip-certified-antibodies/histone-h3k4me3-antibody-snap-chip-certified-cutana-cut-run-compatible\"\n >13-0041</a\n >). High, medium, and low intensity are shown in red,\n yellow, and blue, respectively. Rabbit IgG negative control\n antibody displayed minimal background, as expected.\n </span>\n </p>\n </div>\n <div class=\"image-picker__main-content\">\n <div class=\"image-picker__header-content\">\n <button class=\"image-picker__left-arrow\">\n <svg\n class=\"image-picker__svg-left\"\n width=\"24\"\n height=\"24\"\n viewBox=\"0 0 24 24\">\n <path\n d=\"M16.67 0l2.83 2.829-9.339 9.175 9.339 9.167-2.83 2.829-12.17-11.996z\" />\n </svg>\n </button>\n <a\n href=\"/content/images/products/antibodies/13-0042_CUT&RUN.jpg\"\n target=\"_new\"\n class=\"image-picker__main-image-link\"\n ><img\n loading=\"lazy\"\n alt=\"13-0042 CUT&RUN Data\"\n src=\"/content/images/products/antibodies/13-0042_CUT&RUN.jpg\"\n class=\"image-picker__main-image\" />\n <span class=\"image-picker__main-image-caption\"\n >(Click to enlarge)</span\n ></a\n >\n <button class=\"image-picker__right-arrow\">\n <svg\n class=\"image-picker__svg-right\"\n width=\"24\"\n height=\"24\"\n viewBox=\"0 0 24 24\">\n <path\n d=\"M7.33 24l-2.83-2.829 9.339-9.175-9.339-9.167 2.83-2.829 12.17 11.996z\" />\n </svg>\n </button>\n </div>\n <p>\n <span class=\"image-picker__span-content\"\n ><strong>Figure 2: Rabbit IgG CUT&RUN tracks</strong><br />\n CUT&RUN data was generated as described in\n <strong>Figure 1</strong>. Gene browser shots generated\n using the Integrative Genomics Viewer (IGV, Broad Institute)\n show representative loci for H3K4me3 positive control\n antibody (EpiCypher\n <a\n href=\"https://www.epicypher.com/products/antibodies/snap-chip-certified-antibodies/histone-h3k4me3-antibody-snap-chip-certified-cutana-cut-run-compatible\"\n >13-0041</a\n >). IgG track displayed minimal background, as expected.\n </span>\n </p>\n </div>\n <div class=\"image-picker__main-content\">\n <div class=\"image-picker__header-content\">\n <button class=\"image-picker__left-arrow\">\n <svg\n class=\"image-picker__svg-left\"\n width=\"24\"\n height=\"24\"\n viewBox=\"0 0 24 24\">\n <path\n d=\"M16.67 0l2.83 2.829-9.339 9.175 9.339 9.167-2.83 2.829-12.17-11.996z\" />\n </svg>\n </button>\n <a\n href=\"/content/images/products/antibodies/13-0042-immunoprecipitation.jpeg\"\n target=\"_new\"\n class=\"image-picker__main-image-link\"\n ><img\n loading=\"lazy\"\n alt=\"13-0042-immunoprecipitation\"\n src=\"/content/images/products/antibodies/13-0042-immunoprecipitation.jpeg\"\n class=\"image-picker__main-image\" />\n <span class=\"image-picker__main-image-caption\"\n >(Click to enlarge)</span\n ></a\n >\n <button class=\"image-picker__right-arrow\">\n <svg\n class=\"image-picker__svg-right\"\n width=\"24\"\n height=\"24\"\n viewBox=\"0 0 24 24\">\n <path\n d=\"M7.33 24l-2.83-2.829 9.339-9.175-9.339-9.167 2.83-2.829 12.17 11.996z\" />\n </svg>\n </button>\n </div>\n <p>\n <span class=\"image-picker__span-content\"\n ><strong>Figure 3: Protein A Immunoprecipitation</strong\n ><br />\n Representative dataset to demonstrate functional binding to\n Protein A. Rabbit IgG Antibody (2 µg) was incubated with\n Protein A-conjugated magnetic beads in CUT&RUN Antibody\n Buffer (20 mM HEPES pH 7.5, 150 mM NaCl, 0.5 mM Spermidine,\n 1x Protease Inhibitor, 0.01% Digitonin, 2 mM EDTA) at\n 4°C overnight. One-fifth of the unbound flow-through\n fraction (20% FT) was retained for analysis. The\n antibody-bead complexes were washed three times with CUT&RUN\n Digitonin Buffer (20 mM HEPES pH 7.5, 150 mM NaCl, 0.5 mM\n Spermidine, 1x Protease Inhibitor, 0.01% Digitonin). The\n antibody was eluted by resuspending beads in 1X SDS Buffer\n and heating at 95°C for 15min. Antibody was analyzed by\n SDS-PAGE using Oriole Fluorescent gel staining.\n </span>\n </p>\n </div>\n </div>\n <aside class=\"image-picker__right\">\n <div class=\"image-picker__gallery\">\n <img\n loading=\"lazy\"\n alt=\"13-0042 Heat Map\"\n src=\"/content/images/products/antibodies/13-0042_heatmap.jpg\"\n width=\"200\"\n class=\"image-picker__side-image image-picker__side-image_active\"\n role=\"button\" />\n <img\n loading=\"lazy\"\n alt=\"13-0042 CUT&RUN Data\"\n src=\"/content/images/products/antibodies/13-0042_CUT&RUN.jpg\"\n class=\"image-picker__side-image\"\n role=\"button\" />\n <img\n loading=\"lazy\"\n alt=\"13-0042-immunoprecipitation\"\n src=\"/content/images/products/antibodies/13-0042-immunoprecipitation.jpeg\"\n class=\"image-picker__side-image\"\n role=\"button\" />\n </div>\n </aside>\n </section>\n </div>\n </div>\n </div>\n <div id=\"prodAccordion\">\n <div id=\"ProductDescription\" class=\"Block Panel\">\n <h3 class=\"sub-title1\">Technical Information</h3>\n <div\n class=\"ProductDescriptionContainer product-droppdown__section-description\">\n <div class=\"product-tech-info\">\n <div class=\"product-tech-info__line-item\">\n <div class=\"product-tech-info__line-item-left\">\n <b>Immunogen</b>\n </div>\n <div class=\"product-tech-info__line-item-right\">None</div>\n </div>\n <div class=\"product-tech-info__line-item\">\n <div class=\"product-tech-info__line-item-left\">\n <b>Formulation</b>\n </div>\n <div class=\"product-tech-info__line-item-right\">\n Affinity-purified antibody in PBS pH 7.6\n </div>\n </div>\n <div class=\"product-tech-info__line-item\">\n <div class=\"product-tech-info__line-item-left\">\n <b>Storage and Stability</b>\n </div>\n <div class=\"product-tech-info__line-item-right\">\n Stable for 1 year at 4°C from date of receipt\n </div>\n </div>\n </div>\n </div>\n </div>\n </div>\n <div id=\"prodAccordion\">\n <div id=\"ProductDescription\" class=\"Block Panel\">\n <h3 class=\"sub-title1\">Recommended Dilution</h3>\n <div\n class=\"ProductDescriptionContainer product-droppdown__section-description\">\n <p>\n Prior to use, prepare a 1 mg/mL working stock dilution in CUT&RUN\n Antibody Buffer (20 mM HEPES pH 7.5, 150 mM NaCl, 0.5 mM Spermidine,\n 0.01% Digitonin, 2 mM EDTA). Store working stock at 4°C for 1 month.\n Use 0.5 µg of the working stock for each CUT&RUN assay. A typical\n yield for a CUT&RUN assay using 500,000 K562 cells is 2-5 ng DNA.\n </p>\n </div>\n </div>\n </div>\n <div id=\"prodAccordion\">\n <div id=\"ProductDescription\" class=\"Block Panel\">\n <h3 class=\"sub-title1\">Documents & Resources</h3>\n <div\n class=\"ProductDescriptionContainer product-droppdown__section-description\">\n <div class=\"product-documents\">\n <a\n href=\"/content/documents/tds/13-0042.pdf\"\n target=\"_new\"\n class=\"product-documents__link\">\n <svg\n version=\"1.1\"\n id=\"Layer_1\"\n xmlns=\"http://www.w3.org/2000/svg\"\n xmlns:xlink=\"http://www.w3.org/1999/xlink\"\n x=\"0px\"\n y=\"0px\"\n viewBox=\"0 0 228 240\"\n style=\"enable-background: new 0 0 228 240\"\n xml:space=\"preserve\"\n class=\"product-documents__icon\">\n <g>\n <path\n class=\"product-documents__svg-pdf\"\n d=\"M191.92,68.77l-47.69-47.69c-1.33-1.33-3.12-2.08-5.01-2.08H45.09C41.17,19,38,22.17,38,26.09v184.36\n\t\t\t c0,3.92,3.17,7.09,7.09,7.09h141.82c3.92,0,7.09-3.17,7.09-7.09V73.8C194,71.92,193.25,70.1,191.92,68.77z M177.65,77.06h-41.7\n\t\t\t v-41.7L177.65,77.06z M178.05,201.59H53.95V34.95h66.92v47.86c0,5.14,4.17,9.31,9.31,9.31h47.86V201.59z\" />\n </g>\n <rect\n x=\"20\"\n y=\"112\"\n class=\"product-documents__svg-background\"\n width=\"146\"\n height=\"76\" />\n <g>\n <path\n class=\"product-documents__svg-pdf\"\n d=\"M23.83,125.68h22.36c5.29,0,9.41,1.33,12.35,4c2.94,2.67,4.42,6.39,4.42,11.18c0,4.78-1.47,8.51-4.42,11.18\n\t\t\t c-2.94,2.67-7.06,4-12.35,4H34.59v18.29H23.83V125.68z M44.81,147.9c5.38,0,8.07-2.32,8.07-6.97c0-2.39-0.67-4.16-2-5.31\n\t\t\t c-1.33-1.15-3.36-1.73-6.07-1.73H34.59v14.01H44.81z\" />\n <path\n class=\"product-documents__svg-pdf\"\n d=\"M69.92,125.68h18.91c5.29,0,9.84,0.97,13.66,2.9c3.82,1.93,6.74,4.72,8.76,8.35\n\t\t\t c2.02,3.63,3.04,7.98,3.04,13.04c0,5.06-1,9.42-3,13.08c-2,3.66-4.91,6.45-8.73,8.38c-3.82,1.93-8.4,2.9-13.73,2.9H69.92V125.68z\n\t\t\t M88.07,165.63c10.35,0,15.52-5.22,15.52-15.66c0-10.4-5.17-15.59-15.52-15.59h-7.38v31.26H88.07z\" />\n <path\n class=\"product-documents__svg-pdf\"\n d=\"M122.57,125.68h32.84v8.49h-22.22v11.18h20.84v8.49h-20.84v20.49h-10.63V125.68z\" />\n </g>\n </svg>\n <span class=\"product-documents__info\">Technical Datasheet</span>\n </a>\n </div>\n </div>\n </div>\n </div>\n <div id=\"prodAccordion\">\n <div id=\"ProductDescription\" class=\"Block Panel current\">\n <h3 id=\"bioz\" class=\"sub-title1\">Product References</h3>\n <div\n class=\"ProductDescriptionContainer product-droppdown__section-description-specific\">\n <object\n id=\"wobj-3835-13-0042-q\"\n type=\"text/html\"\n data=\"https://www.bioz.com/v_widget_6_0/3835/13-0042/?ex=1\"\n style=\"width: 100%; height: 193px\"></object>\n <div id=\"bioz-w-pb-3835-13-0042-q-div\" style=\"width: 100%\">\n <a\n id=\"bioz-w-pb-3835-13-0042-q\"\n style=\"font-size: 12px; text-decoration: none; color: #4698cb\"\n href=\"https://www.bioz.com/\"\n target=\"_blank\"\n ><img\n src=\"https://cdn.bioz.com/assets/favicon.png\"\n style=\"\n width: 11px;\n height: 11px;\n vertical-align: baseline;\n padding-bottom: 0px;\n margin-left: 0px;\n margin-bottom: 0px;\n float: none;\n \" />\n Powered by Bioz</a\n >\n <a\n style=\"\n font-size: 12px;\n text-decoration: none;\n float: right;\n color: transparent;\n \"\n href=\"https://www.bioz.com/result/13-0042/product/EpiCypher/?cn=14-1048\"\n target=\"_blank\">\n See more details on Bioz</a\n >\n </div>\n </div>\n </div>\n </div>\n </div>\n</div>\n\n<script>\n $(document).ready(function () {\n var widget_micro_obj = new v_widget_obj('s', [1]);\n widget_micro_obj.request_catalog_number_widget_data_internal(\n '13-0042',\n '13-0042'\n );\n });\n</script>\n\n<style>\n .form-field-title .required-text {\n display: none !important;\n }\n\n .form-label {\n padding-left: 2rem;\n }\n\n .form-label-text {\n margin: 0;\n margin-left: 0 !important;\n }\n\n /* //////////// */\n\n /* BIOZ */\n td table {\n margin: 0;\n padding: 6px !important;\n }\n\n span.bioz-w-parent-hover {\n font-size: 15px;\n }\n\n td .bioz-w-tooltipx {\n padding-top: 0 !important;\n }\n /* .bioz-w-parent-hover:hover {\n text-decoration: underline white 2px !important;\n } */\n\n /* /////////// */\n\n .form-field-title .required-text {\n display: none !important;\n }\n .form-label {\n padding-left: 2rem;\n }\n .form-label-text {\n margin: 0;\n margin-left: 0 !important;\n }\n\n .bioz-w-header td {\n padding: 0;\n }\n\n .epicypher-table table {\n margin-right: auto;\n margin-left: auto;\n width: 100%;\n border-top: 1px solid lightgray;\n }\n\n .epicypher-table th {\n color: #3b3a48;\n }\n .epicypher-table td {\n color: #3b3a48;\n font-size: 0.9rem;\n }\n .epicypher-table td a {\n text-decoration: underline;\n }\n .epicypher-table td,\n th {\n border: 0.5px solid lightgray;\n text-align: left;\n padding: 8px;\n /* white-space: nowrap; */\n }\n .epicypher-table tr:nth-child(even) {\n background-color: #f9f9f9;\n }\n .epicypher-table td:first-child {\n border-left: 1px solid lightgray;\n }\n\n /* Tablet */\n\n @media only screen and (max-width: 1024px) {\n /* Force table to not be like tables anymore */\n .epicypher-table th {\n display: none !important;\n }\n\n /* BIOZ */\n td.bioz-w-tooltipx::before {\n display: none;\n }\n\n /* Hide table headers (but not display: none;, for accessibility) */\n .epicypher-table thead tr {\n position: absolute;\n top: -9999px;\n left: -9999px;\n }\n\n .epicypher-table tr {\n border: 1px solid #ccc;\n }\n\n .epicypher-table td {\n /* Behave like a \"row\" */\n /* border: none; */\n border-bottom: 1px solid #eee;\n position: relative;\n padding-left: 30%;\n white-space: inherit;\n }\n\n .epicypher-table td:before {\n /* Now like a table header */\n position: absolute;\n /* Top/left values mimic padding */\n top: 6px;\n left: 6px;\n width: 45%;\n padding-right: 10px;\n white-space: nowrap;\n font-weight: 700;\n }\n\n /*\n Label the data\n */\n .epicypher-table td:nth-of-type(1):before {\n content: 'Item';\n }\n td:nth-of-type(2):before {\n content: 'Cat. No.';\n }\n /* BIOZ */\n }\n\n @media screen and (max-width: 767px) {\n .custom-title-description {\n font-size: 19px !important;\n }\n\n .section-title {\n font-size: 19px !important;\n padding-left: 1rem;\n }\n\n }\n</style>\n","tags":[],"warranty":"","price":{"without_tax":{"formatted":"$85.00","value":85,"currency":"USD"},"tax_label":"Sales Tax"},"detail_messages":"","availability":"","page_title":"CUTANA™ Rabbit IgG CUT&RUN Negative Control Antibody","cart_url":"https://www.epicypher.com/cart.php","max_purchase_quantity":0,"mpn":null,"upc":null,"options":[],"related_products":[{"id":694,"sku":null,"name":"CUTANA™ pAG-MNase for ChIC/CUT&RUN Workflows","url":"https://www.epicypher.com/products/epigenetics-reagents-and-assays/cutana-pag-mnase-for-chic-cut-and-run-workflows","availability":"","rating":null,"brand":{"name":null},"category":["Epigenetics Kits and Reagents","Epigenetics Kits and Reagents/CUTANA™ ChIC / CUT&RUN Assays"],"summary":"\n \n \n Type: Nuclease\n \n \n Mol Wgt: 43.7 kDa\n \n \n \n ","image":{"data":"https://cdn11.bigcommerce.com/s-y9o92/images/stencil/{:size}/products/694/689/Screen_Shot_2020-02-12_at_11.01.55_AM__17144.1581530752.png?c=2","alt":"CUTANA™ pAG-MNase for ChIC/CUT&RUN Workflows"},"images":[{"data":"https://cdn11.bigcommerce.com/s-y9o92/images/stencil/{:size}/products/694/689/Screen_Shot_2020-02-12_at_11.01.55_AM__17144.1581530752.png?c=2","alt":"CUTANA™ pAG-MNase for ChIC/CUT&RUN Workflows"}],"date_added":"12th Aug 2019","pre_order":false,"show_cart_action":true,"has_options":true,"stock_level":null,"low_stock_level":null,"qty_in_cart":0,"custom_fields":[{"id":1174,"name":"Internal Comment","value":"Excess in bottom of Venom"},{"id":1175,"name":"Internal Comment","value":"Bulk in Psylocke"}],"num_reviews":null,"weight":{"formatted":"0.01 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- Type: Polyclonal
- Host: Rabbit
- Applications: CUT&RUN
- Reactivity: Negative Control
- Format: Affinity Purified IgG
- Target Size: N/A
Description
This Rabbit IgG Antibody can be used to control for non-specific background signal in CUT&RUN and other applications. Pair with the H3K4me3 positive control antibody (EpiCypher 13-0041) for a well-controlled experiment.
Validation Data
Figure 1: CUT&RUN genome wide enrichment
CUT&RUN data was generated using the EpiCypher CUTANA™
CUT&RUN
protocol. Sequence reads were aligned to 18,793 annotated
transcription start sites (TSSs ± 2 kbp). Signal enrichment
was sorted from highest to lowest (top to bottom) relative
to H3K4me3 CUT&RUN positive control antibody (EpiCypher
13-0041). High, medium, and low intensity are shown in red,
yellow, and blue, respectively. Rabbit IgG negative control
antibody displayed minimal background, as expected.
Figure 2: Rabbit IgG CUT&RUN tracks
CUT&RUN data was generated as described in
Figure 1. Gene browser shots generated
using the Integrative Genomics Viewer (IGV, Broad Institute)
show representative loci for H3K4me3 positive control
antibody (EpiCypher
13-0041). IgG track displayed minimal background, as expected.
Figure 3: Protein A Immunoprecipitation
Representative dataset to demonstrate functional binding to
Protein A. Rabbit IgG Antibody (2 µg) was incubated with
Protein A-conjugated magnetic beads in CUT&RUN Antibody
Buffer (20 mM HEPES pH 7.5, 150 mM NaCl, 0.5 mM Spermidine,
1x Protease Inhibitor, 0.01% Digitonin, 2 mM EDTA) at
4°C overnight. One-fifth of the unbound flow-through
fraction (20% FT) was retained for analysis. The
antibody-bead complexes were washed three times with CUT&RUN
Digitonin Buffer (20 mM HEPES pH 7.5, 150 mM NaCl, 0.5 mM
Spermidine, 1x Protease Inhibitor, 0.01% Digitonin). The
antibody was eluted by resuspending beads in 1X SDS Buffer
and heating at 95°C for 15min. Antibody was analyzed by
SDS-PAGE using Oriole Fluorescent gel staining.
Technical Information
Recommended Dilution
Prior to use, prepare a 1 mg/mL working stock dilution in CUT&RUN Antibody Buffer (20 mM HEPES pH 7.5, 150 mM NaCl, 0.5 mM Spermidine, 0.01% Digitonin, 2 mM EDTA). Store working stock at 4°C for 1 month. Use 0.5 µg of the working stock for each CUT&RUN assay. A typical yield for a CUT&RUN assay using 500,000 K562 cells is 2-5 ng DNA.