SKU: 15-1017
{"AddThisServiceButtonMeta":"","add_this":[{"annotation":"","service":"facebook"},{"annotation":"","service":"email"},{"annotation":"","service":"print"},{"annotation":"","service":"twitter"},{"annotation":"","service":"linkedin"}],"add_to_wishlist_url":"/wishlist.php?action=add&product_id=764","availability":"","bulk_discount_rates":[],"can_purchase":true,"cart_url":"https://www.epicypher.com/cart.php","category":["Epigenetics Kits and Reagents","Epigenetics Kits and Reagents/CUTANA™ CUT&Tag Assays"],"custom_fields":[{"id":"1005","name":"Internal Comment","value":"extra boxes in bottom shelf of Venom"}],"customizations":[],"description":"<div class=\"product-general-info\">\n <ul class=\"product-general-info__list-left\">\n <li class=\"product-general-info__list-item\">\n <strong>Type: </strong>Transposase\n </li>\n <li class=\"product-general-info__list-item\">\n <strong>Mol Wgt: </strong>191 kDa\n </li>\n </ul>\n <ul class=\"product-general-info__list-right\">\n <li class=\"product-general-info__list-item\">\n <strong>Host: </strong><em>E. coli</em>\n </li>\n <li class=\"product-general-info__list-item\">\n <strong>Epitope Tag: </strong>None\n </li>\n </ul>\n</div>\n<div class=\"service_accordion product-droppdown\">\n <div class=\"container\">\n <div id=\"prodAccordion\">\n <div id=\"ProductDescription\" class=\"Block Panel current\">\n <h3 class=\"sub-title1\">Description</h3>\n <div\n class=\"ProductDescriptionContainer product-droppdown__section-description-specific\"\n style=\"display: block\"\n >\n <p>\n Available in 50 and 250 Reaction pack sizes. Recombinantly produced\n in <em>E. coli</em>, CUTANA pAG-Tn5 for CUT&Tag is a fusion of\n Proteins A and G to Transposase Tn5. This construct is useful in\n performing Cleavage Under Targets and Tagmentation (CUT&Tag).\n The active dimer of Transposase Tn5 is charged with Illumina\n adapters and ready to be used immediately in CUT&Tag. CUTANA\n pAG-Tn5 does not contain an epitope tag, which makes it compatible\n with CUT&Tag for epitope tagged proteins (FLAG, HA, TY1, etc.).\n To place bulk orders, contact\n <a href=\"mailto:info@epicypher.com\">info@epicypher.com</a>.\n </p>\n </div>\n </div>\n </div>\n <div id=\"prodAccordion\">\n <div id=\"ProductDescription\" class=\"Block Panel current\">\n <h3 class=\"sub-title1\">Validation Data</h3>\n <div\n class=\"ProductDescriptionContainer product-droppdown__section-description-specific\"\n style=\"display: block\"\n >\n <section class=\"image-picker\">\n <div class=\"image-picker__left\">\n <div\n class=\"image-picker__main-content_active image-picker__main-content\"\n >\n <div class=\"image-picker__header-content\">\n <button class=\"image-picker__left-arrow\">\n <svg\n class=\"image-picker__svg-left\"\n width=\"24\"\n height=\"24\"\n viewBox=\"0 0 24 24\"\n >\n <path\n d=\"M16.67 0l2.83 2.829-9.339 9.175 9.339 9.167-2.83 2.829-12.17-11.996z\"\n />\n </svg>\n </button>\n <a\n href=\"/content/images/products/proteins/15_1017_Protein_Gel.jpg\"\n target=\"blank\"\n class=\"image-picker__main-image-link\"\n ><img\n alt=\"15-1017 Heatmap\"\n src=\"/content/images/products/proteins/15_1017_Protein_Gel.jpg\"\n class=\"image-picker__main-image\"\n />\n <span class=\"image-picker__main-image-caption\"\n >(Click to enlarge)</span\n ></a\n >\n <button class=\"image-picker__right-arrow\">\n <svg\n class=\"image-picker__svg-right\"\n width=\"24\"\n height=\"24\"\n viewBox=\"0 0 24 24\"\n >\n <path\n d=\"M7.33 24l-2.83-2.829 9.339-9.175-9.339-9.167 2.83-2.829 12.17 11.996z\"\n />\n </svg>\n </button>\n </div>\n <p>\n <span class=\"image-picker__span-content\"\n ><strong>Figure 1: Protein Gel Data </strong><br />CUTANA\n pAG-Tn5 for CUT&Tag (1 µg) was resolved via SDS-PAGE and\n stained with Coomassie blue. The migration and molecular\n weight of the protein standards are indicated.\n </span>\n </p>\n </div>\n <div class=\"image-picker__main-content\">\n <div class=\"image-picker__header-content\">\n <button class=\"image-picker__left-arrow\">\n <svg\n class=\"image-picker__svg-left\"\n width=\"24\"\n height=\"24\"\n viewBox=\"0 0 24 24\"\n >\n <path\n d=\"M16.67 0l2.83 2.829-9.339 9.175 9.339 9.167-2.83 2.829-12.17-11.996z\"\n />\n </svg>\n </button>\n <a\n href=\"/content/images/products/proteins/15_1017_BioA_data.jpg\"\n target=\"blank\"\n class=\"image-picker__main-image-link\"\n ><img\n alt=\"15-1017 Bio Data\"\n src=\"/content/images/products/proteins/15_1017_BioA_data.jpg\"\n class=\"image-picker__main-image\"\n />\n <span class=\"image-picker__main-image-caption\"\n >(Click to enlarge)</span\n ></a\n >\n <button class=\"image-picker__right-arrow\">\n <svg\n class=\"image-picker__svg-right\"\n width=\"24\"\n height=\"24\"\n viewBox=\"0 0 24 24\"\n >\n <path\n d=\"M7.33 24l-2.83-2.829 9.339-9.175-9.339-9.167 2.83-2.829 12.17 11.996z\"\n />\n </svg>\n </button>\n </div>\n <p>\n <span class=\"image-picker__span-content\"\n ><strong\n >Figure 2: Size Distribution of Released Chromatin </strong\n ><br />\n CUT&Tag was performed using CUTANA pAG-Tn5 (1:20\n dilution) with 100,000 K-562 nuclei. Recovered DNA was\n directly PCR amplified to produce sequence-ready libraries.\n Agilent Bioanalyzer traces for libraries derived from\n negative control Rabbit IgG (left; EpiCypher Catalog No.\n <a\n href=\"/products/epigenetics-reagents-and-assays/cutana-chic-cut-run-assays/cutana-rabbit-igg-cut-run-negative-control-antibody\"\n target=\"blank\"\n >13-0042</a\n >, Lot 20036001-52) and H3K27me3 (right; EpiCypher Catalog\n No.\n <a\n href=\"/products/antibodies/snap-chip-certified-antibodies/histone-h3k27me3-antibody-snap-chip-certified-cutana-cut-run-compatible-discontinued\"\n target=\"blank\"\n >13-0030</a\n >, Lot 18303001) are shown. Excised DNA is highly enriched\n for mononucleosomes (peak at ~300 bp reflects 150 bp insert\n size).\n </span>\n </p>\n </div>\n <div class=\"image-picker__main-content\">\n <div class=\"image-picker__header-content\">\n <button class=\"image-picker__left-arrow\">\n <svg\n class=\"image-picker__svg-left\"\n width=\"24\"\n height=\"24\"\n viewBox=\"0 0 24 24\"\n >\n <path\n d=\"M16.67 0l2.83 2.829-9.339 9.175 9.339 9.167-2.83 2.829-12.17-11.996z\"\n />\n </svg>\n </button>\n <a\n href=\"/content/images/products/proteins/15_1017_C&T_data.jpg\"\n target=\"blank\"\n class=\"image-picker__main-image-link\"\n >\n <img\n alt=\"15_1017_C&T_data\"\n src=\"/content/images/products/proteins/15_1017_C&T_data.jpg\"\n class=\"image-picker__main-image\"\n />\n <span class=\"image-picker__main-image-caption\"\n >(Click to enlarge)</span\n >\n </a>\n <button class=\"image-picker__right-arrow\">\n <svg\n class=\"image-picker__svg-right\"\n width=\"24\"\n height=\"24\"\n viewBox=\"0 0 24 24\"\n >\n <path\n d=\"M7.33 24l-2.83-2.829 9.339-9.175-9.339-9.167 2.83-2.829 12.17 11.996z\"\n />\n </svg>\n </button>\n </div>\n <p>\n <span class=\"image-picker__span-content\">\n <strong>Figure 3: CUT&Tag Data </strong><br />\n Representative sequencing tracks obtained using CUTANA\n pAG-Tn5 are shown for two different loci: a 300 kb close up\n view of the LAMC3 gene <strong>(A)</strong> and a wide 5,608\n kb window <strong>(B)</strong>. CUT&Tag was performed\n using 100,000 K-562 nuclei with H3K4me3 antibody (EpiCypher\n Catalog No.\n <a\n href=\"/products/antibodies/snap-chip-certified-antibodies/histone-h3k4me3-antibody-snap-chip-certified-cutana-cut-run-compatible\"\n target=\"blank\"\n >13-0041</a\n >, Lot 20083002-42), H3K27me3 antibody (EpiCypher Catalog\n No.\n <a\n href=\"/products/antibodies/snap-chip-certified-antibodies/histone-h3k27me3-antibody-snap-chip-certified-cutana-cut-run-compatible-discontinued\"\n target=\"blank\"\n >13-0030</a\n >, Lot 18303001) and negative control Rabbit IgG (EpiCypher\n Catalog No.\n <a\n href=\"/products/epigenetics-reagents-and-assays/cutana-chic-cut-run-assays/cutana-rabbit-igg-cut-run-negative-control-antibody\"\n target=\"blank\"\n >13-0042</a\n >, Lot 20036001-52). Total paired-end read counts were 0.7,\n 1.1, and 0.9 million reads for H3K4me3, H3K27me3, and IgG,\n respectively. Images were generated using the Integrative\n Genomics Viewer (IGV, Broad Institute). CUTANA pAG-Tn5\n produced clear peaks with genomic distribution profiles\n consistent with the known biological functions of H3K4me3\n and H3K27me3.\n </span>\n </p>\n </div>\n </div>\n <aside class=\"image-picker__right\">\n <div class=\"image-picker__gallery\">\n <img\n alt=\"15-1017 Protein-Gel\"\n src=\"/content/images/products/proteins/15_1017_Protein_Gel.jpg\"\n width=\"200\"\n class=\"image-picker__side-image image-picker__side-image_active\"\n role=\"button\"\n />\n <img\n alt=\"15-1017 BioA\"\n src=\"/content/images/products/proteins/15_1017_BioA_data.jpg\"\n class=\"image-picker__side-image\"\n role=\"button\"\n />\n <img\n alt=\"15-1017 C&T\"\n src=\"/content/images/products/proteins/15_1017_C&T_data.jpg\"\n class=\"image-picker__side-image\"\n role=\"button\"\n />\n </div>\n </aside>\n </section>\n </div>\n </div>\n </div>\n <div id=\"prodAccordion\">\n <div id=\"ProductDescription\" class=\"Block Panel\">\n <h3 class=\"sub-title1\">Technical Information</h3>\n <div\n class=\"ProductDescriptionContainer product-droppdown__section-description\"\n >\n <div class=\"product-tech-info\">\n <!-- <div class=\"product-tech-info__line-item\">\n <div class=\"product-tech-info__line-item-left\">\n <b>Immunogen</b>\n </div>\n \n <div class=\"product-tech-info__line-item-right\">\n A synthetic peptide corresponding to human SNF2L amino acids\n 1004 to 1054.\n </div>\n </div> -->\n <div class=\"product-tech-info__line-item\">\n <div class=\"product-tech-info__line-item-left\">\n <b>Formulation:</b>\n </div>\n <div class=\"product-tech-info__line-item-right\">\n CUTANA pAG-Tn5 is provided as a 20X stock in storage buffer (50\n mM HEPES-KOH pH 7.2, 100 mM NaCl, 0.1 mM EDTA, 1 mM DTT, 0.1%\n Triton X-100, 50% glycerol)\n </div>\n </div>\n <div class=\"product-tech-info__line-item\">\n <div class=\"product-tech-info__line-item-left\">\n <b>Storage and Stability:</b>\n </div>\n <div class=\"product-tech-info__line-item-right\">\n Stable for one year at -20°C from date of receipt. The protein\n is not subject to freeze/thaw under these conditions.\n </div>\n </div>\n </div>\n </div>\n </div>\n </div>\n <div id=\"prodAccordion\">\n <div id=\"ProductDescription\" class=\"Block Panel\">\n <h3 class=\"sub-title1\">Application Notes</h3>\n <div\n class=\"ProductDescriptionContainer product-droppdown__section-description\"\n >\n <p>\n <strong>Recommended Use:</strong> 2.5 µL of the supplied enzyme into\n a 50 µL CUT&Tag reaction (20X dilution). For detailed applications\n and uses of this product, please see our\n <a\n href=\"/content/documents/protocols/cutana-cut&tag-protocol.pdf\"\n target=\"blank\"\n >CUT&Tag protocol</a\n >.\n </p>\n <p>\n <strong>Adapter Sequences:</strong><br />\n Tn5ME-A: 5’-TCGTCGGCAGCGTCAGATGTGTATAAGAGACAG-3’ <br />\n\n Tn5ME-B: 5’-GTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG-3’ <br />\n\n Tn5MErev: 5’-[phos]CTGTCTCTTATACACATCT-3’<br />\n </p>\n <p>\n <strong>Critical Note:</strong> Since CUT&Tag has lower\n background and is compatible with fewer cells compared to ChIP-seq,\n it is not recommended to assess fragment size distribution using\n agarose gel or capillary electrophoresis (<i>e.g.</i> Agilent\n Bioanalyzer or TapeStation) prior to library amplification. This\n analysis is not indicative of the success of a CUT&Tag\n experiment, and further the amount of DNA recovered may be below the\n sensitivity of detection for these approaches. To gauge the success\n of a CUT&Tag experiment, assess DNA yield compared to positive\n (<i>e.g.</i> H3K4me3, H3K27me3) and negative (IgG) controls,\n determine fragment size distribution of sequence-ready libraries,\n and evaluate peak structure and expected genome-wide distribution in\n NGS data. For more detailed information, please see our\n <a\n href=\"/content/documents/protocols/cutana-cut&tag-protocol.pdf\"\n target=\"blank\"\n >CUT&Tag protocol.</a\n >\n </p>\n </div>\n </div>\n </div>\n <div id=\"prodAccordion\">\n <div id=\"ProductDescription\" class=\"Block Panel\">\n <h3 class=\"sub-title1\">References</h3>\n <div\n class=\"ProductDescriptionContainer product-droppdown__section-description\"\n >\n <b>Background References:</b><br />\n Henikoff <em>et al.</em> <em>eLife</em> (2020) PMID:\n <a\n href=\"https://pubmed.ncbi.nlm.nih.gov/33191916/\"\n title=\"Profiling the epigenome at home\"\n target=\"new\"\n >33191916</a\n ><br />\n Kaya-Okur <em>et al.</em> <em>Nat. Commun.</em> (2019) PMID:\n <a\n href=\"https://pubmed.ncbi.nlm.nih.gov/31036827/\"\n title=\"CUT&Tag for efficient epigenomic profiling of small samples and single cells\"\n target=\"new\"\n >31036827</a\n ><br />\n <br />\n <strong>Product References:</strong>\n <div>\n <iframe\n class=\"airtable-embed\"\n src=\"https://airtable.com/embed/shr4aG2p59KazCtSj?backgroundColor=purple&viewControls=on\"\n frameborder=\"0\"\n onmousewheel=\"\"\n width=\"100%\"\n height=\"300\"\n style=\"background: transparent; border: 1px solid #ccc\"\n ></iframe>\n </div>\n </div>\n </div>\n </div>\n <div id=\"prodAccordion\">\n <div id=\"ProductDescription\" class=\"Block Panel\">\n <h3 class=\"sub-title1\">Documents & Resources</h3>\n <div\n class=\"ProductDescriptionContainer product-droppdown__section-description\"\n >\n <div class=\"product-documents\">\n <a\n href=\"/content/documents/tds/15-1017.pdf\"\n target=\"blank\"\n class=\"product-documents__link\"\n >\n <svg\n version=\"1.1\"\n 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xmlns:xlink=\"http://www.w3.org/1999/xlink\"\n x=\"0px\"\n y=\"0px\"\n viewBox=\"0 0 228 240\"\n style=\"enable-background: new 0 0 228 240\"\n xml:space=\"preserve\"\n class=\"product-documents__icon\"\n >\n <g>\n <path\n class=\"product-documents__svg-pdf\"\n d=\"M191.92,68.77l-47.69-47.69c-1.33-1.33-3.12-2.08-5.01-2.08H45.09C41.17,19,38,22.17,38,26.09v184.36\n c0,3.92,3.17,7.09,7.09,7.09h141.82c3.92,0,7.09-3.17,7.09-7.09V73.8C194,71.92,193.25,70.1,191.92,68.77z M177.65,77.06h-41.7\n v-41.7L177.65,77.06z M178.05,201.59H53.95V34.95h66.92v47.86c0,5.14,4.17,9.31,9.31,9.31h47.86V201.59z\"\n />\n </g>\n <rect\n x=\"20\"\n y=\"112\"\n class=\"product-documents__svg-background\"\n width=\"146\"\n height=\"76\"\n />\n <g>\n <path\n class=\"product-documents__svg-pdf\"\n d=\"M23.83,125.68h22.36c5.29,0,9.41,1.33,12.35,4c2.94,2.67,4.42,6.39,4.42,11.18c0,4.78-1.47,8.51-4.42,11.18\n c-2.94,2.67-7.06,4-12.35,4H34.59v18.29H23.83V125.68z M44.81,147.9c5.38,0,8.07-2.32,8.07-6.97c0-2.39-0.67-4.16-2-5.31\n c-1.33-1.15-3.36-1.73-6.07-1.73H34.59v14.01H44.81z\"\n />\n <path\n class=\"product-documents__svg-pdf\"\n d=\"M69.92,125.68h18.91c5.29,0,9.84,0.97,13.66,2.9c3.82,1.93,6.74,4.72,8.76,8.35\n c2.02,3.63,3.04,7.98,3.04,13.04c0,5.06-1,9.42-3,13.08c-2,3.66-4.91,6.45-8.73,8.38c-3.82,1.93-8.4,2.9-13.73,2.9H69.92V125.68z\n M88.07,165.63c10.35,0,15.52-5.22,15.52-15.66c0-10.4-5.17-15.59-15.52-15.59h-7.38v31.26H88.07z\"\n />\n <path\n class=\"product-documents__svg-pdf\"\n d=\"M122.57,125.68h32.84v8.49h-22.22v11.18h20.84v8.49h-20.84v20.49h-10.63V125.68z\"\n />\n </g>\n </svg>\n <span class=\"product-documents__info\"\n >CUT&Tag Protocol for Histone PTMs</span\n >\n </a>\n </div>\n <div class=\"product-documents\">\n <a\n href=\"/resources/protocols/cutana-cut-and-tag-protocol/\"\n target=\"blank\"\n class=\"product-documents__link\"\n >\n <svg\n version=\"1.1\"\n id=\"Layer_1\"\n xmlns=\"http://www.w3.org/2000/svg\"\n 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