

CUTANA™ pAG-Tn5 for CUT&Tag Workflows
{"AddThisServiceButtonMeta":"","add_this":[{"annotation":"","service":"facebook"},{"annotation":"","service":"email"},{"annotation":"","service":"print"},{"annotation":"","service":"twitter"},{"annotation":"","service":"linkedin"}],"add_to_wishlist_url":"/wishlist.php?action=add&product_id=764","availability":"","bulk_discount_rates":[],"can_purchase":true,"cart_url":"https://www.epicypher.com/cart.php","category":["Epigenetics Kits and Reagents","Epigenetics Kits and Reagents/CUTANA™ CUT&Tag Assays"],"custom_fields":[{"id":"1005","name":"Internal Comment","value":"extra boxes in bottom shelf of Venom"}],"customizations":[],"description":"<div class=\"product-general-info\">\n <ul class=\"product-general-info__list-left\">\n <li class=\"product-general-info__list-item\">\n <strong>Type: </strong>Transposase\n </li>\n <li class=\"product-general-info__list-item\">\n <strong>Mol Wgt: </strong>191 kDa\n </li>\n </ul>\n <ul class=\"product-general-info__list-right\">\n <li class=\"product-general-info__list-item\">\n <strong>Host: </strong><em>E. coli</em>\n </li>\n <li class=\"product-general-info__list-item\">\n <strong>Epitope Tag: </strong>None\n </li>\n </ul>\n</div>\n<div class=\"service_accordion product-droppdown\">\n <div class=\"container\">\n <div id=\"prodAccordion\">\n <div id=\"ProductDescription\" class=\"Block Panel current\">\n <h3 class=\"sub-title1\">Description</h3>\n <div\n class=\"ProductDescriptionContainer product-droppdown__section-description-specific\"\n style=\"display: block\"\n >\n <p>\n Available in 50 and 250 Reaction pack sizes. Recombinantly produced\n in <em>E. coli</em>, CUTANA pAG-Tn5 for CUT&Tag is a fusion of\n Proteins A and G to Transposase Tn5. This construct is useful in\n performing Cleavage Under Targets and Tagmentation (CUT&Tag).\n The active dimer of Transposase Tn5 is charged with Illumina\n adapters and ready to be used immediately in CUT&Tag. CUTANA\n pAG-Tn5 does not contain an epitope tag, which makes it compatible\n with CUT&Tag for epitope tagged proteins (FLAG, HA, TY1, etc.).\n To place bulk orders, contact\n <a href=\"mailto:info@epicypher.com\">info@epicypher.com</a>.\n </p>\n </div>\n </div>\n </div>\n <div id=\"prodAccordion\">\n <div id=\"ProductDescription\" class=\"Block Panel current\">\n <h3 class=\"sub-title1\">Validation Data</h3>\n <div\n class=\"ProductDescriptionContainer product-droppdown__section-description-specific\"\n style=\"display: block\"\n >\n <section class=\"image-picker\">\n <div class=\"image-picker__left\">\n <div\n class=\"image-picker__main-content_active image-picker__main-content\"\n >\n <div class=\"image-picker__header-content\">\n <button class=\"image-picker__left-arrow\">\n <svg\n class=\"image-picker__svg-left\"\n width=\"24\"\n height=\"24\"\n viewBox=\"0 0 24 24\"\n >\n <path\n d=\"M16.67 0l2.83 2.829-9.339 9.175 9.339 9.167-2.83 2.829-12.17-11.996z\"\n />\n </svg>\n </button>\n <a\n href=\"/content/images/products/proteins/15_1017_Protein_Gel.jpg\"\n target=\"blank\"\n class=\"image-picker__main-image-link\"\n ><img\n alt=\"15-1017 Heatmap\"\n src=\"/content/images/products/proteins/15_1017_Protein_Gel.jpg\"\n class=\"image-picker__main-image\"\n />\n <span class=\"image-picker__main-image-caption\"\n >(Click to enlarge)</span\n ></a\n >\n <button class=\"image-picker__right-arrow\">\n <svg\n class=\"image-picker__svg-right\"\n width=\"24\"\n height=\"24\"\n viewBox=\"0 0 24 24\"\n >\n <path\n d=\"M7.33 24l-2.83-2.829 9.339-9.175-9.339-9.167 2.83-2.829 12.17 11.996z\"\n />\n </svg>\n </button>\n </div>\n <p>\n <span class=\"image-picker__span-content\"\n ><strong>Figure 1: Protein Gel Data </strong><br />CUTANA\n pAG-Tn5 for CUT&Tag (1 µg) was resolved via SDS-PAGE and\n stained with Coomassie blue. The migration and molecular\n weight of the protein standards are indicated.\n </span>\n </p>\n </div>\n <div class=\"image-picker__main-content\">\n <div class=\"image-picker__header-content\">\n <button class=\"image-picker__left-arrow\">\n <svg\n class=\"image-picker__svg-left\"\n width=\"24\"\n height=\"24\"\n viewBox=\"0 0 24 24\"\n >\n <path\n d=\"M16.67 0l2.83 2.829-9.339 9.175 9.339 9.167-2.83 2.829-12.17-11.996z\"\n />\n </svg>\n </button>\n <a\n href=\"/content/images/products/proteins/15_1017_BioA_data.jpg\"\n target=\"blank\"\n class=\"image-picker__main-image-link\"\n ><img\n alt=\"15-1017 Bio Data\"\n src=\"/content/images/products/proteins/15_1017_BioA_data.jpg\"\n class=\"image-picker__main-image\"\n />\n <span class=\"image-picker__main-image-caption\"\n >(Click to enlarge)</span\n ></a\n >\n <button class=\"image-picker__right-arrow\">\n <svg\n class=\"image-picker__svg-right\"\n width=\"24\"\n height=\"24\"\n viewBox=\"0 0 24 24\"\n >\n <path\n d=\"M7.33 24l-2.83-2.829 9.339-9.175-9.339-9.167 2.83-2.829 12.17 11.996z\"\n />\n </svg>\n </button>\n </div>\n <p>\n <span class=\"image-picker__span-content\"\n ><strong\n >Figure 2: Size Distribution of Released Chromatin </strong\n ><br />\n CUT&Tag was performed using CUTANA pAG-Tn5 (1:20\n dilution) with 100,000 K-562 nuclei. Recovered DNA was\n directly PCR amplified to produce sequence-ready libraries.\n Agilent Bioanalyzer traces for libraries derived from\n negative control Rabbit IgG (left; EpiCypher Catalog No.\n <a\n href=\"/products/epigenetics-reagents-and-assays/cutana-chic-cut-run-assays/cutana-rabbit-igg-cut-run-negative-control-antibody\"\n target=\"blank\"\n >13-0042</a\n >, Lot 20036001-52) and H3K27me3 (right; EpiCypher Catalog\n No.\n <a\n href=\"/products/antibodies/snap-chip-certified-antibodies/histone-h3k27me3-antibody-snap-chip-certified-cutana-cut-run-compatible-discontinued\"\n target=\"blank\"\n >13-0030</a\n >, Lot 18303001) are shown. Excised DNA is highly enriched\n for mononucleosomes (peak at ~300 bp reflects 150 bp insert\n size).\n </span>\n </p>\n </div>\n <div class=\"image-picker__main-content\">\n <div class=\"image-picker__header-content\">\n <button class=\"image-picker__left-arrow\">\n <svg\n class=\"image-picker__svg-left\"\n width=\"24\"\n height=\"24\"\n viewBox=\"0 0 24 24\"\n >\n <path\n d=\"M16.67 0l2.83 2.829-9.339 9.175 9.339 9.167-2.83 2.829-12.17-11.996z\"\n />\n </svg>\n </button>\n <a\n href=\"/content/images/products/proteins/15_1017_C&T_data.jpg\"\n target=\"blank\"\n class=\"image-picker__main-image-link\"\n >\n <img\n alt=\"15_1017_C&T_data\"\n src=\"/content/images/products/proteins/15_1017_C&T_data.jpg\"\n class=\"image-picker__main-image\"\n />\n <span class=\"image-picker__main-image-caption\"\n >(Click to enlarge)</span\n >\n </a>\n <button class=\"image-picker__right-arrow\">\n <svg\n class=\"image-picker__svg-right\"\n width=\"24\"\n height=\"24\"\n viewBox=\"0 0 24 24\"\n >\n <path\n d=\"M7.33 24l-2.83-2.829 9.339-9.175-9.339-9.167 2.83-2.829 12.17 11.996z\"\n />\n </svg>\n </button>\n </div>\n <p>\n <span class=\"image-picker__span-content\">\n <strong>Figure 3: CUT&Tag Data </strong><br />\n Representative sequencing tracks obtained using CUTANA\n pAG-Tn5 are shown for two different loci: a 300 kb close up\n view of the LAMC3 gene <strong>(A)</strong> and a wide 5,608\n kb window <strong>(B)</strong>. CUT&Tag was performed\n using 100,000 K-562 nuclei with H3K4me3 antibody (EpiCypher\n Catalog No.\n <a\n href=\"/products/antibodies/snap-chip-certified-antibodies/histone-h3k4me3-antibody-snap-chip-certified-cutana-cut-run-compatible\"\n target=\"blank\"\n >13-0041</a\n >, Lot 20083002-42), H3K27me3 antibody (EpiCypher Catalog\n No.\n <a\n href=\"/products/antibodies/snap-chip-certified-antibodies/histone-h3k27me3-antibody-snap-chip-certified-cutana-cut-run-compatible-discontinued\"\n target=\"blank\"\n >13-0030</a\n >, Lot 18303001) and negative control Rabbit IgG (EpiCypher\n Catalog No.\n <a\n href=\"/products/epigenetics-reagents-and-assays/cutana-chic-cut-run-assays/cutana-rabbit-igg-cut-run-negative-control-antibody\"\n target=\"blank\"\n >13-0042</a\n >, Lot 20036001-52). Total paired-end read counts were 0.7,\n 1.1, and 0.9 million reads for H3K4me3, H3K27me3, and IgG,\n respectively. Images were generated using the Integrative\n Genomics Viewer (IGV, Broad Institute). CUTANA pAG-Tn5\n produced clear peaks with genomic distribution profiles\n consistent with the known biological functions of H3K4me3\n and H3K27me3.\n </span>\n </p>\n </div>\n </div>\n <aside class=\"image-picker__right\">\n <div class=\"image-picker__gallery\">\n <img\n alt=\"15-1017 Protein-Gel\"\n src=\"/content/images/products/proteins/15_1017_Protein_Gel.jpg\"\n width=\"200\"\n class=\"image-picker__side-image image-picker__side-image_active\"\n role=\"button\"\n />\n <img\n alt=\"15-1017 BioA\"\n src=\"/content/images/products/proteins/15_1017_BioA_data.jpg\"\n class=\"image-picker__side-image\"\n role=\"button\"\n />\n <img\n alt=\"15-1017 C&T\"\n src=\"/content/images/products/proteins/15_1017_C&T_data.jpg\"\n class=\"image-picker__side-image\"\n role=\"button\"\n />\n </div>\n </aside>\n </section>\n </div>\n </div>\n </div>\n <div id=\"prodAccordion\">\n <div id=\"ProductDescription\" class=\"Block Panel\">\n <h3 class=\"sub-title1\">Technical Information</h3>\n <div\n class=\"ProductDescriptionContainer product-droppdown__section-description\"\n >\n <div class=\"product-tech-info\">\n <!-- <div class=\"product-tech-info__line-item\">\n <div class=\"product-tech-info__line-item-left\">\n <b>Immunogen</b>\n </div>\n \n <div class=\"product-tech-info__line-item-right\">\n A synthetic peptide corresponding to human SNF2L amino acids\n 1004 to 1054.\n </div>\n </div> -->\n <div class=\"product-tech-info__line-item\">\n <div class=\"product-tech-info__line-item-left\">\n <b>Formulation:</b>\n </div>\n <div class=\"product-tech-info__line-item-right\">\n CUTANA pAG-Tn5 is provided as a 20X stock in storage buffer (50\n mM HEPES-KOH pH 7.2, 100 mM NaCl, 0.1 mM EDTA, 1 mM DTT, 0.1%\n Triton X-100, 50% glycerol)\n </div>\n </div>\n <div class=\"product-tech-info__line-item\">\n <div class=\"product-tech-info__line-item-left\">\n <b>Storage and Stability:</b>\n </div>\n <div class=\"product-tech-info__line-item-right\">\n Stable for one year at -20°C from date of receipt. The protein\n is not subject to freeze/thaw under these conditions.\n </div>\n </div>\n </div>\n </div>\n </div>\n </div>\n <div id=\"prodAccordion\">\n <div id=\"ProductDescription\" class=\"Block Panel\">\n <h3 class=\"sub-title1\">Application Notes</h3>\n <div\n class=\"ProductDescriptionContainer product-droppdown__section-description\"\n >\n <p>\n <strong>Recommended Use:</strong> 2.5 µL of the supplied enzyme into\n a 50 µL CUT&Tag reaction (20X dilution). For detailed applications\n and uses of this product, please see our\n <a\n href=\"/content/documents/protocols/cutana-cut&tag-protocol.pdf\"\n target=\"blank\"\n >CUT&Tag protocol</a\n >.\n </p>\n <p>\n <strong>Adapter Sequences:</strong><br />\n Tn5ME-A: 5’-TCGTCGGCAGCGTCAGATGTGTATAAGAGACAG-3’ <br />\n\n Tn5ME-B: 5’-GTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG-3’ <br />\n\n Tn5MErev: 5’-[phos]CTGTCTCTTATACACATCT-3’<br />\n </p>\n <p>\n <strong>Critical Note:</strong> Since CUT&Tag has lower\n background and is compatible with fewer cells compared to ChIP-seq,\n it is not recommended to assess fragment size distribution using\n agarose gel or capillary electrophoresis (<i>e.g.</i> Agilent\n Bioanalyzer or TapeStation) prior to library amplification. This\n analysis is not indicative of the success of a CUT&Tag\n experiment, and further the amount of DNA recovered may be below the\n sensitivity of detection for these approaches. To gauge the success\n of a CUT&Tag experiment, assess DNA yield compared to positive\n (<i>e.g.</i> H3K4me3, H3K27me3) and negative (IgG) controls,\n determine fragment size distribution of sequence-ready libraries,\n and evaluate peak structure and expected genome-wide distribution in\n NGS data. For more detailed information, please see our\n <a\n href=\"/content/documents/protocols/cutana-cut&tag-protocol.pdf\"\n target=\"blank\"\n >CUT&Tag protocol.</a\n >\n </p>\n </div>\n </div>\n </div>\n <div id=\"prodAccordion\">\n <div id=\"ProductDescription\" class=\"Block Panel\">\n <h3 class=\"sub-title1\">References</h3>\n <div\n class=\"ProductDescriptionContainer product-droppdown__section-description\"\n >\n <b>Background References:</b><br />\n Henikoff <em>et al.</em> <em>eLife</em> (2020) PMID:\n <a\n href=\"https://pubmed.ncbi.nlm.nih.gov/33191916/\"\n title=\"Profiling the epigenome at home\"\n target=\"new\"\n >33191916</a\n ><br />\n Kaya-Okur <em>et al.</em> <em>Nat. Commun.</em> (2019) PMID:\n <a\n href=\"https://pubmed.ncbi.nlm.nih.gov/31036827/\"\n title=\"CUT&Tag for efficient epigenomic profiling of small samples and single cells\"\n target=\"new\"\n >31036827</a\n ><br />\n <br />\n <strong>Product References:</strong>\n <div>\n <iframe\n class=\"airtable-embed\"\n src=\"https://airtable.com/embed/shr4aG2p59KazCtSj?backgroundColor=purple&viewControls=on\"\n frameborder=\"0\"\n onmousewheel=\"\"\n width=\"100%\"\n height=\"300\"\n style=\"background: transparent; border: 1px solid #ccc\"\n ></iframe>\n </div>\n </div>\n </div>\n </div>\n <div id=\"prodAccordion\">\n <div id=\"ProductDescription\" class=\"Block Panel\">\n <h3 class=\"sub-title1\">Documents & Resources</h3>\n <div\n class=\"ProductDescriptionContainer product-droppdown__section-description\"\n >\n <div class=\"product-documents\">\n <a\n href=\"/content/documents/tds/15-1017.pdf\"\n target=\"blank\"\n class=\"product-documents__link\"\n >\n <svg\n version=\"1.1\"\n id=\"Layer_1\"\n xmlns=\"http://www.w3.org/2000/svg\"\n xmlns:xlink=\"http://www.w3.org/1999/xlink\"\n x=\"0px\"\n y=\"0px\"\n viewBox=\"0 0 228 240\"\n style=\"enable-background: new 0 0 228 240\"\n xml:space=\"preserve\"\n class=\"product-documents__icon\"\n >\n <g>\n <path\n class=\"product-documents__svg-pdf\"\n d=\"M191.92,68.77l-47.69-47.69c-1.33-1.33-3.12-2.08-5.01-2.08H45.09C41.17,19,38,22.17,38,26.09v184.36\n c0,3.92,3.17,7.09,7.09,7.09h141.82c3.92,0,7.09-3.17,7.09-7.09V73.8C194,71.92,193.25,70.1,191.92,68.77z M177.65,77.06h-41.7\n v-41.7L177.65,77.06z M178.05,201.59H53.95V34.95h66.92v47.86c0,5.14,4.17,9.31,9.31,9.31h47.86V201.59z\"\n />\n </g>\n <rect\n x=\"20\"\n y=\"112\"\n class=\"product-documents__svg-background\"\n width=\"146\"\n height=\"76\"\n />\n <g>\n <path\n class=\"product-documents__svg-pdf\"\n d=\"M23.83,125.68h22.36c5.29,0,9.41,1.33,12.35,4c2.94,2.67,4.42,6.39,4.42,11.18c0,4.78-1.47,8.51-4.42,11.18\n c-2.94,2.67-7.06,4-12.35,4H34.59v18.29H23.83V125.68z M44.81,147.9c5.38,0,8.07-2.32,8.07-6.97c0-2.39-0.67-4.16-2-5.31\n c-1.33-1.15-3.36-1.73-6.07-1.73H34.59v14.01H44.81z\"\n />\n <path\n class=\"product-documents__svg-pdf\"\n d=\"M69.92,125.68h18.91c5.29,0,9.84,0.97,13.66,2.9c3.82,1.93,6.74,4.72,8.76,8.35\n c2.02,3.63,3.04,7.98,3.04,13.04c0,5.06-1,9.42-3,13.08c-2,3.66-4.91,6.45-8.73,8.38c-3.82,1.93-8.4,2.9-13.73,2.9H69.92V125.68z\n M88.07,165.63c10.35,0,15.52-5.22,15.52-15.66c0-10.4-5.17-15.59-15.52-15.59h-7.38v31.26H88.07z\"\n />\n <path\n class=\"product-documents__svg-pdf\"\n d=\"M122.57,125.68h32.84v8.49h-22.22v11.18h20.84v8.49h-20.84v20.49h-10.63V125.68z\"\n />\n </g>\n </svg>\n <span class=\"product-documents__info\">Technical Datasheet</span>\n </a>\n </div>\n <div class=\"product-documents\">\n <a\n href=\"/content/documents/protocols/cutana-cut&tag-protocol.pdf\"\n target=\"blank\"\n class=\"product-documents__link\"\n >\n <svg\n version=\"1.1\"\n id=\"Layer_1\"\n xmlns=\"http://www.w3.org/2000/svg\"\n xmlns:xlink=\"http://www.w3.org/1999/xlink\"\n x=\"0px\"\n y=\"0px\"\n viewBox=\"0 0 228 240\"\n style=\"enable-background: new 0 0 228 240\"\n xml:space=\"preserve\"\n class=\"product-documents__icon\"\n >\n <g>\n <path\n class=\"product-documents__svg-pdf\"\n d=\"M191.92,68.77l-47.69-47.69c-1.33-1.33-3.12-2.08-5.01-2.08H45.09C41.17,19,38,22.17,38,26.09v184.36\n c0,3.92,3.17,7.09,7.09,7.09h141.82c3.92,0,7.09-3.17,7.09-7.09V73.8C194,71.92,193.25,70.1,191.92,68.77z M177.65,77.06h-41.7\n v-41.7L177.65,77.06z M178.05,201.59H53.95V34.95h66.92v47.86c0,5.14,4.17,9.31,9.31,9.31h47.86V201.59z\"\n />\n </g>\n <rect\n x=\"20\"\n y=\"112\"\n class=\"product-documents__svg-background\"\n width=\"146\"\n height=\"76\"\n />\n <g>\n <path\n class=\"product-documents__svg-pdf\"\n d=\"M23.83,125.68h22.36c5.29,0,9.41,1.33,12.35,4c2.94,2.67,4.42,6.39,4.42,11.18c0,4.78-1.47,8.51-4.42,11.18\n c-2.94,2.67-7.06,4-12.35,4H34.59v18.29H23.83V125.68z M44.81,147.9c5.38,0,8.07-2.32,8.07-6.97c0-2.39-0.67-4.16-2-5.31\n c-1.33-1.15-3.36-1.73-6.07-1.73H34.59v14.01H44.81z\"\n />\n <path\n class=\"product-documents__svg-pdf\"\n d=\"M69.92,125.68h18.91c5.29,0,9.84,0.97,13.66,2.9c3.82,1.93,6.74,4.72,8.76,8.35\n c2.02,3.63,3.04,7.98,3.04,13.04c0,5.06-1,9.42-3,13.08c-2,3.66-4.91,6.45-8.73,8.38c-3.82,1.93-8.4,2.9-13.73,2.9H69.92V125.68z\n M88.07,165.63c10.35,0,15.52-5.22,15.52-15.66c0-10.4-5.17-15.59-15.52-15.59h-7.38v31.26H88.07z\"\n />\n <path\n class=\"product-documents__svg-pdf\"\n d=\"M122.57,125.68h32.84v8.49h-22.22v11.18h20.84v8.49h-20.84v20.49h-10.63V125.68z\"\n />\n </g>\n </svg>\n <span class=\"product-documents__info\"\n >CUT&Tag Protocol for Histone PTMs</span\n >\n </a>\n </div>\n <div class=\"product-documents\">\n <a\n href=\"/resources/protocols/cutana-cut-and-tag-protocol/\"\n target=\"blank\"\n class=\"product-documents__link\"\n >\n <svg\n version=\"1.1\"\n id=\"Layer_1\"\n xmlns=\"http://www.w3.org/2000/svg\"\n xmlns:xlink=\"http://www.w3.org/1999/xlink\"\n x=\"0px\"\n y=\"0px\"\n viewBox=\"0 0 228 240\"\n style=\"enable-background: new 0 0 228 240\"\n xml:space=\"preserve\"\n class=\"product-documents__icon\"\n >\n <g>\n <path\n class=\"product-documents__svg-link\"\n d=\"M130.3,157.06c-0.77-0.76-2-0.76-2.77,0l-28.45,28.45c-13.17,13.17-35.41,14.57-49.95,0\n c-14.57-14.57-13.17-36.78,0-49.95l28.45-28.45c0.76-0.76,0.76-2.01,0-2.77l-9.75-9.75c-0.77-0.76-2-0.76-2.77,0l-28.45,28.45\n c-20.71,20.71-20.71,54.23,0,74.92s54.23,20.71,74.92,0L140,169.52c0.76-0.76,0.76-2.01,0-2.77L130.3,157.06z M193.62,41\n c-20.71-20.71-54.23-20.71-74.92,0L90.22,69.45c-0.76,0.77-0.76,2,0,2.77l9.72,9.72c0.76,0.76,2.01,0.76,2.77,0l28.45-28.45\n c13.17-13.17,35.41-14.57,49.95,0c14.57,14.57,13.17,36.78,0,49.95l-28.45,28.45c-0.76,0.77-0.76,2,0,2.77l9.75,9.75\n c0.76,0.76,2.01,0.76,2.77,0l28.45-28.45C214.31,95.23,214.31,61.71,193.62,41L193.62,41z M139.14,85.29c-0.77-0.76-2-0.76-2.77,0\n l-55.46,55.43c-0.76,0.77-0.76,2,0,2.77l9.7,9.7c0.76,0.76,2.01,0.76,2.77,0l55.43-55.43c0.76-0.76,0.76-2.01,0-2.77L139.14,85.29z\n \"\n />\n </g>\n </svg>\n <span class=\"product-documents__info\"\n >Previous CUTANA<sup>™</sup> CUT&Tag Protocol versions</span\n >\n </a>\n </div>\n </div>\n </div>\n </div>\n </div>\n</div>\n","detail_messages":"","gift_wrapping_available":false,"gtin":"","id":"764","images":[{"alt":"CUTANA™ pAG-Tn5 for CUT&Tag Workflows","data":"https://cdn11.bigcommerce.com/s-y9o92/images/stencil/{:size}/products/764/750/2020_CUTTag_icon_RGB_Andy_L__93238.1592491196.png?c=2"}],"main_image":{"alt":"CUTANA™ pAG-Tn5 for CUT&Tag Workflows","data":"https://cdn11.bigcommerce.com/s-y9o92/images/stencil/{:size}/products/764/750/2020_CUTTag_icon_RGB_Andy_L__93238.1592491196.png?c=2"},"max_purchase_quantity":0,"meta_description":"CUTANA™ pAG-Tn5 for CUT&Tag","meta_keywords":"CUTANA™ pAG-Tn5 for CUT&Tag, cutandtag, cutana, chic, chromatin immunocleavage, Cleavage Under Targets and Tagmentation, 15-1017","min_purchase_quantity":0,"mpn":"","num_reviews":0,"options":[{"condition":true,"display_name":"Select Pack Size:","id":1210,"partial":"set-radio","required":true,"state":"variant_option","type":"Configurable_PickList_Set","values":[{"data":"50 Reactions","id":161,"label":"50 Reactions","selected":true},{"data":"250 Reactions","id":162,"label":"250 Reactions","selected":false}]}],"page_title":"CUTANA™ pAG-Tn5 for CUT&Tag","price":{"tax_label":"Sales Tax","without_tax":{"currency":"USD","formatted":"$695.00","value":695}},"rating":0,"related_products":[{"add_to_wishlist_url":"/wishlist.php?action=add&product_id=694","availability":"","brand":{"name":null},"category":["Nucleosomes/SNAP-CUTANA™ Spike-in Controls","Epigenetics Kits and Reagents/CUTANA™ ChIC / CUT&RUN Assays"],"custom_fields":null,"date_added":"12th Aug 2019","demo":false,"has_options":true,"id":694,"image":{"alt":"CUTANA™ pAG-MNase for ChIC/CUT&RUN Workflows","data":"https://cdn11.bigcommerce.com/s-y9o92/images/stencil/{:size}/products/694/689/Screen_Shot_2020-02-12_at_11.01.55_AM__17144.1581530752.png?c=2"},"images":[{"alt":"CUTANA™ pAG-MNase for ChIC/CUT&RUN Workflows","data":"https://cdn11.bigcommerce.com/s-y9o92/images/stencil/{:size}/products/694/689/Screen_Shot_2020-02-12_at_11.01.55_AM__17144.1581530752.png?c=2"}],"low_stock_level":null,"name":"CUTANA™ pAG-MNase for ChIC/CUT&RUN Workflows","num_reviews":null,"pre_order":false,"price":{"price_range":{"max":{"tax_label":"Sales Tax","without_tax":{"currency":"USD","formatted":"$1,250.00","value":1250}},"min":{"tax_label":"Sales Tax","without_tax":{"currency":"USD","formatted":"$300.00","value":300}}},"tax_label":"Sales Tax","without_tax":{"currency":"USD","formatted":"$300.00","value":300}},"qty_in_cart":0,"rating":null,"show_cart_action":true,"sku":null,"stock_level":null,"summary":"\n \n \n Type: Nuclease\n \n \n Mol Wgt: 43.7 kDa\n \n \n \n ","url":"https://www.epicypher.com/products/epigenetics-reagents-and-assays/cutana-pag-mnase-for-chic-cut-and-run-workflows","weight":{"formatted":"0.01 LBS","value":0.01}},{"add_to_wishlist_url":"/wishlist.php?action=add&product_id=770","availability":"","brand":{"name":null},"category":["Epigenetics Kits and Reagents/CUTANA™ CUT&Tag Assays"],"custom_fields":null,"date_added":"23rd Jul 2020","demo":false,"has_options":true,"id":770,"image":{"alt":"Anti-Rabbit Secondary Antibody for CUTANA™ CUT&Tag Workflows","data":"https://cdn11.bigcommerce.com/s-y9o92/images/stencil/{:size}/products/770/756/2020_CUTTag_icon_RGB_Andy_L__75299.1592353722.1280.1280__51372.1594752212.1280.1280__02658.1595531327.jpg?c=2"},"images":[{"alt":"Anti-Rabbit Secondary Antibody for CUTANA™ CUT&Tag Workflows","data":"https://cdn11.bigcommerce.com/s-y9o92/images/stencil/{:size}/products/770/756/2020_CUTTag_icon_RGB_Andy_L__75299.1592353722.1280.1280__51372.1594752212.1280.1280__02658.1595531327.jpg?c=2"}],"low_stock_level":null,"name":"Anti-Rabbit Secondary Antibody for CUTANA™ CUT&Tag Workflows","num_reviews":null,"pre_order":false,"price":{"price_range":{"max":{"tax_label":"Sales Tax","without_tax":{"currency":"USD","formatted":"$255.00","value":255}},"min":{"tax_label":"Sales Tax","without_tax":{"currency":"USD","formatted":"$75.00","value":75}}},"tax_label":"Sales Tax","without_tax":{"currency":"USD","formatted":"$75.00","value":75}},"qty_in_cart":0,"rating":null,"show_cart_action":true,"sku":null,"stock_level":null,"summary":"\n \n \n Type: Mixed Monoclonal*\n \n \n Host: Goat\n \n \n Applications: ","url":"https://www.epicypher.com/products/epigenetics-reagents-and-assays/cutana-cut-tag-assays/anti-rabbit-secondary-antibody-for-cutana-cut-tag-workflows","weight":{"formatted":"0.01 LBS","value":0.01}},{"add_to_wishlist_url":"/wishlist.php?action=add&product_id=771","availability":"","brand":{"name":null},"category":["Epigenetics Kits and Reagents/CUTANA™ CUT&Tag Assays"],"custom_fields":[{"id":986,"name":"Internal Comment","value":"extra located top-right of Venom"}],"date_added":"23rd Jul 2020","demo":false,"has_options":true,"id":771,"image":{"alt":"Anti-Mouse Secondary Antibody for CUTANA CUTandTag Workflows","data":"https://cdn11.bigcommerce.com/s-y9o92/images/stencil/{:size}/products/771/943/anti-mouse-secondary-antibody-for-cutana-cutandtag-workflows__06671.1645734534.jpg?c=2"},"images":[{"alt":"Anti-Mouse Secondary Antibody for CUTANA CUTandTag Workflows","data":"https://cdn11.bigcommerce.com/s-y9o92/images/stencil/{:size}/products/771/943/anti-mouse-secondary-antibody-for-cutana-cutandtag-workflows__06671.1645734534.jpg?c=2"}],"low_stock_level":null,"name":"Anti-Mouse Secondary Antibody for CUTANA™ CUT&Tag Workflows ","num_reviews":null,"pre_order":false,"price":{"price_range":{"max":{"tax_label":"Sales Tax","without_tax":{"currency":"USD","formatted":"$255.00","value":255}},"min":{"tax_label":"Sales Tax","without_tax":{"currency":"USD","formatted":"$75.00","value":75}}},"tax_label":"Sales Tax","without_tax":{"currency":"USD","formatted":"$75.00","value":75}},"qty_in_cart":0,"rating":null,"show_cart_action":true,"sku":null,"stock_level":null,"summary":"\n \n \n Type: Mixed Monoclonal*\n \n \n Host: Goat\n \n \n Applications: ","url":"https://www.epicypher.com/products/epigenetics-reagents-and-assays/cutanac-cut-tag-assays/anti-mouse-secondary-antibody-for-cutana-cut-tag-workflows","weight":{"formatted":"0.01 LBS","value":0.01}},{"add_to_cart_url":"https://www.epicypher.com/cart.php?action=add&product_id=875","add_to_wishlist_url":"/wishlist.php?action=add&product_id=875","availability":"","brand":{"name":null},"category":["Antibodies/CUTANA™ CUT&RUN Antibodies","Antibodies/CUTANA™ CUT&RUN Antibodies/CUT&RUN Antibodies - Chromatin-Associated Proteins","Epigenetics Kits and Reagents/CUTANA™ ChIC / CUT&RUN Assays"],"custom_fields":[{"id":909,"name":"Pack Size","value":"100 µg"}],"date_added":"21st Jul 2021","demo":false,"has_options":false,"id":875,"image":{"alt":"HA Tag CUTANA CUTandRUN Antibody","data":"https://cdn11.bigcommerce.com/s-y9o92/images/stencil/{:size}/products/875/937/ha-tag-cutana-cutandrun-antibody__43540.1645734529.jpg?c=2"},"images":[{"alt":"HA Tag CUTANA CUTandRUN Antibody","data":"https://cdn11.bigcommerce.com/s-y9o92/images/stencil/{:size}/products/875/937/ha-tag-cutana-cutandrun-antibody__43540.1645734529.jpg?c=2"}],"low_stock_level":null,"name":"HA Tag CUTANA™ CUT&RUN Antibody","num_reviews":null,"pre_order":false,"price":{"tax_label":"Sales Tax","without_tax":{"currency":"USD","formatted":"$445.00","value":445}},"qty_in_cart":0,"rating":null,"show_cart_action":true,"sku":"13-2010","stock_level":null,"summary":"\n \n \n Type: Polyclonal\n \n \n Host: Rabbit\n \n \n Target Size: N/A\n ","url":"https://www.epicypher.com/products/antibodies/cutana-cut-run-compatible-antibodies/ha-tag-cutana-cut-and-run-antibody","weight":{"formatted":"0.00 LBS","value":0}},{"add_to_cart_url":"https://www.epicypher.com/cart.php?action=add&product_id=890","add_to_wishlist_url":"/wishlist.php?action=add&product_id=890","availability":"","brand":{"name":null},"category":["Epigenetics Kits and Reagents","Epigenetics Kits and Reagents/CUTANA™ CUT&Tag Assays"],"custom_fields":[{"id":984,"name":"Pack Size","value":"50 Reactions"}],"date_added":"31st Jan 2022","demo":false,"has_options":false,"id":890,"image":{"alt":"CUTANA™ CUT&Tag Core Reagents Bundle","data":"https://cdn11.bigcommerce.com/s-y9o92/images/stencil/{:size}/products/890/918/2020_CUTTag_icon_RGB_Andy_L__93238.1592491196__35422.1643742241.png?c=2"},"images":[{"alt":"CUTANA™ CUT&Tag Core Reagents Bundle","data":"https://cdn11.bigcommerce.com/s-y9o92/images/stencil/{:size}/products/890/918/2020_CUTTag_icon_RGB_Andy_L__93238.1592491196__35422.1643742241.png?c=2"}],"low_stock_level":null,"name":"CUTANA™ CUT&Tag Core Reagents Bundle","num_reviews":null,"pre_order":false,"price":{"tax_label":"Sales Tax","without_tax":{"currency":"USD","formatted":"$1,295.00","value":1295}},"qty_in_cart":0,"rating":null,"show_cart_action":true,"sku":"14-1101","stock_level":null,"summary":"\n \n \n \n Description\n \n \n The CUTANA™ CUT&Tag Core Reagents Bundle","url":"https://www.epicypher.com/products/epigenetics-kits-and-reagents/cutana-cut-tag-core-reagents-bundle","weight":{"formatted":"0.01 LBS","value":0.01}}],"shipping":{"calculated":true},"shipping_messages":[],"show_quantity_input":1,"sku":"15-1017","tags":[],"title":"CUTANA™ pAG-Tn5 for CUT&Tag Workflows","upc":null,"url":"https://www.epicypher.com/products/epigenetics-kits-and-reagents/cutana-pag-tn5-for-cut-and-tag","warranty":"","weight":"0.01 LBS"}
- Type: Transposase
- Mol Wgt: 191 kDa
- Host: E. coli
- Epitope Tag: None
Description
Available in 50 and 250 Reaction pack sizes. Recombinantly produced in E. coli, CUTANA pAG-Tn5 for CUT&Tag is a fusion of Proteins A and G to Transposase Tn5. This construct is useful in performing Cleavage Under Targets and Tagmentation (CUT&Tag). The active dimer of Transposase Tn5 is charged with Illumina adapters and ready to be used immediately in CUT&Tag. CUTANA pAG-Tn5 does not contain an epitope tag, which makes it compatible with CUT&Tag for epitope tagged proteins (FLAG, HA, TY1, etc.). To place bulk orders, contact info@epicypher.com.
Validation Data
Figure 1: Protein Gel Data
CUTANA
pAG-Tn5 for CUT&Tag (1 µg) was resolved via SDS-PAGE and
stained with Coomassie blue. The migration and molecular
weight of the protein standards are indicated.
Figure 2: Size Distribution of Released Chromatin
CUT&Tag was performed using CUTANA pAG-Tn5 (1:20
dilution) with 100,000 K-562 nuclei. Recovered DNA was
directly PCR amplified to produce sequence-ready libraries.
Agilent Bioanalyzer traces for libraries derived from
negative control Rabbit IgG (left; EpiCypher Catalog No.
13-0042, Lot 20036001-52) and H3K27me3 (right; EpiCypher Catalog
No.
13-0030, Lot 18303001) are shown. Excised DNA is highly enriched
for mononucleosomes (peak at ~300 bp reflects 150 bp insert
size).
Figure 3: CUT&Tag Data
Representative sequencing tracks obtained using CUTANA
pAG-Tn5 are shown for two different loci: a 300 kb close up
view of the LAMC3 gene (A) and a wide 5,608
kb window (B). CUT&Tag was performed
using 100,000 K-562 nuclei with H3K4me3 antibody (EpiCypher
Catalog No.
13-0041, Lot 20083002-42), H3K27me3 antibody (EpiCypher Catalog
No.
13-0030, Lot 18303001) and negative control Rabbit IgG (EpiCypher
Catalog No.
13-0042, Lot 20036001-52). Total paired-end read counts were 0.7,
1.1, and 0.9 million reads for H3K4me3, H3K27me3, and IgG,
respectively. Images were generated using the Integrative
Genomics Viewer (IGV, Broad Institute). CUTANA pAG-Tn5
produced clear peaks with genomic distribution profiles
consistent with the known biological functions of H3K4me3
and H3K27me3.
Technical Information
Application Notes
Recommended Use: 2.5 µL of the supplied enzyme into a 50 µL CUT&Tag reaction (20X dilution). For detailed applications and uses of this product, please see our CUT&Tag protocol.
Adapter Sequences:
Tn5ME-A: 5’-TCGTCGGCAGCGTCAGATGTGTATAAGAGACAG-3’
Tn5ME-B: 5’-GTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG-3’
Tn5MErev: 5’-[phos]CTGTCTCTTATACACATCT-3’
Critical Note: Since CUT&Tag has lower background and is compatible with fewer cells compared to ChIP-seq, it is not recommended to assess fragment size distribution using agarose gel or capillary electrophoresis (e.g. Agilent Bioanalyzer or TapeStation) prior to library amplification. This analysis is not indicative of the success of a CUT&Tag experiment, and further the amount of DNA recovered may be below the sensitivity of detection for these approaches. To gauge the success of a CUT&Tag experiment, assess DNA yield compared to positive (e.g. H3K4me3, H3K27me3) and negative (IgG) controls, determine fragment size distribution of sequence-ready libraries, and evaluate peak structure and expected genome-wide distribution in NGS data. For more detailed information, please see our CUT&Tag protocol.
Documents & Resources
Related Products


Anti-Rabbit Secondary Antibody for CUTANA™ CUT&Tag Workflows

Anti-Mouse Secondary Antibody for CUTANA™ CUT&Tag Workflows

