SKU: 13-0048
{"url":"https://www.epicypher.com/products/epigenetics-reagents-and-assays/cutanac-cut-tag-assays/anti-mouse-secondary-antibody-for-cutana-cut-tag-workflows","add_this":[{"service":"facebook","annotation":""},{"service":"email","annotation":""},{"service":"print","annotation":""},{"service":"twitter","annotation":""},{"service":"linkedin","annotation":""}],"gtin":"","id":"771","bulk_discount_rates":[],"can_purchase":true,"meta_description":"Validated Anti-Mouse Secondary Antibody for CUTANA™ CUT&Tag Assays","category":["CUTANA™ CUT&Tag Antibodies","Antibodies/CUTANA™ CUT&Tag Antibodies","Epigenetics Kits and Reagents/CUTANA™ CUT&Tag Assays"],"AddThisServiceButtonMeta":"","main_image":{"data":"https://cdn11.bigcommerce.com/s-y9o92/images/stencil/{:size}/products/771/943/anti-mouse-secondary-antibody-for-cutana-cutandtag-workflows__06671.1645734534.jpg?c=2","alt":"Anti-Mouse Secondary Antibody for CUTANA CUTandTag Workflows"},"add_to_wishlist_url":"/wishlist.php?action=add&product_id=771","shipping":{"calculated":true},"num_reviews":0,"weight":"0.01 LBS","custom_fields":[{"id":"986","name":"Internal Comment","value":"extra located top-right of Venom"}],"sku":"13-0048","description":"<div class=\"product-general-info\">\n <ul class=\"product-general-info__list-left\">\n <li class=\"product-general-info__list-item\">\n <strong>Type: </strong>Mixed Monoclonal*\n </li>\n <li class=\"product-general-info__list-item\">\n <strong>Host: </strong>Goat\n </li>\n <li class=\"product-general-info__list-item\">\n <strong>Applications: </strong>CUT&Tag\n </li>\n </ul>\n <ul class=\"product-general-info__list-right\">\n <li class=\"product-general-info__list-item\">\n <strong>Reactivity: </strong>Anti-Mouse\n </li>\n <li class=\"product-general-info__list-item\">\n <strong>Format: </strong>Protein G affinity-purified\n </li>\n <li class=\"product-general-info__list-item\">\n <strong>Target Size: </strong>N/A\n </li>\n </ul>\n</div>\n\n<div class=\"service_accordion product-droppdown\">\n <div class=\"container\">\n <div id=\"prodAccordion\">\n <div id=\"ProductDescription\" class=\"Block Panel current\">\n <h3 class=\"sub-title1\">Description</h3>\n <div\n class=\"ProductDescriptionContainer product-droppdown__section-description-specific\">\n <p>\n Anti-Mouse Secondary Antibody for CUTANA™ CUT&Tag is\n affinity-purified and specific for mouse immunoglobulins. Minimal\n cross reactivity with rabbit, rat, human, bovine, guinea pig, and\n donkey IgG is observed. Use of a secondary antibody in CUT&Tag aids\n in signal amplification by increasing bound IgG per target epitope\n [1]. For best results, use with CUTANA™ pAG-Tn5 (EpiCypher\n <a\n href=\"/products/epigenetics-kits-and-reagents/cutana-pag-tn5-for-cut-and-tag\"\n >15-1017 / 15-1117</a\n >). For detailed instructions for use, see EpiCypher’s CUTANA™\n CUT&Tag <a href=\"/protocols\">protocol</a>.\n </p>\n <p>\n <em\n >*Mixed Monoclonal: a pool of multiple monoclonal antibodies.</em\n >\n </p>\n </div>\n </div>\n </div>\n <div id=\"prodAccordion\">\n <div id=\"ProductDescription\" class=\"Block Panel current\">\n <h3 class=\"sub-title1\">Validation Data</h3>\n <div\n class=\"ProductDescriptionContainer product-droppdown__section-description-specific\">\n <section class=\"image-picker\">\n <div class=\"image-picker__left\">\n <div\n class=\"image-picker__main-content_active image-picker__main-content\">\n <div class=\"image-picker__header-content\">\n <button class=\"image-picker__left-arrow\">\n <svg\n class=\"image-picker__svg-left\"\n width=\"24\"\n height=\"24\"\n viewBox=\"0 0 24 24\">\n <path\n d=\"M16.67 0l2.83 2.829-9.339 9.175 9.339 9.167-2.83 2.829-12.17-11.996z\" />\n </svg>\n </button>\n <a\n href=\"/content/images/products/antibodies/13-0048-cut-tag-data.jpeg\"\n target=\"_blank\"\n class=\"image-picker__main-image-link\"\n ><img\n loading=\"lazy\"\n alt=\"13-0048-cut-tag-data\"\n src=\"/content/images/products/antibodies/13-0048-cut-tag-data.jpeg\"\n class=\"image-picker__main-image\" />\n <span class=\"image-picker__main-image-caption\"\n >(Click to enlarge)</span\n ></a\n >\n <button class=\"image-picker__right-arrow\">\n <svg\n class=\"image-picker__svg-right\"\n width=\"24\"\n height=\"24\"\n viewBox=\"0 0 24 24\">\n <path\n d=\"M7.33 24l-2.83-2.829 9.339-9.175-9.339-9.167 2.83-2.829 12.17 11.996z\" />\n </svg>\n </button>\n </div>\n <p>\n <span class=\"image-picker__span-content\"\n ><strong>Figure 1: CUT&Tag data </strong><br />\n CUT&Tag was performed as described above. Gene browser shots\n generated using the Integrative Genomics Viewer (IGV, Broad\n Institute) show a representative 474 kb window centered at\n the LAMC3 gene. The genomic distribution pattern was\n consistent with that expected for H3K27me3.\n </span>\n </p>\n </div>\n <div class=\"image-picker__main-content\">\n <div class=\"image-picker__header-content\">\n <button class=\"image-picker__left-arrow\">\n <svg\n class=\"image-picker__svg-left\"\n width=\"24\"\n height=\"24\"\n viewBox=\"0 0 24 24\">\n <path\n d=\"M16.67 0l2.83 2.829-9.339 9.175 9.339 9.167-2.83 2.829-12.17-11.996z\" />\n </svg>\n </button>\n <a\n href=\"/content/images/products/methods/cut-and-tag-methods.png\"\n target=\"_new\"\n class=\"image-picker__main-image-link\">\n <img\n alt=\"cut-and-tag-methods\"\n src=\"/content/images/products/methods/cut-and-tag-methods.png\"\n class=\"image-picker__main-image\" />\n <span class=\"image-picker__main-image-caption\"\n >(Click to enlarge)</span\n >\n </a>\n <button class=\"image-picker__right-arrow\">\n <svg\n class=\"image-picker__svg-right\"\n width=\"24\"\n height=\"24\"\n viewBox=\"0 0 24 24\">\n <path\n d=\"M7.33 24l-2.83-2.829 9.339-9.175-9.339-9.167 2.83-2.829 12.17 11.996z\" />\n </svg>\n </button>\n </div>\n <p>\n <span class=\"image-picker__span-content\">\n <strong>CUT&Tag methods</strong><br />\n CUT&Tag was performed on 100k K562 nuclei with 0.5 µg of\n either IgG (Invitrogen 10400C) or H3K27me3 (Abcam ab6002)\n antibodies, followed by 0.5 µg of anti-mouse secondary\n antibody for CUTANA™ CUT&Tag. The EpiCypher Direct-to-PCR\n CUT&Tag <a href=\"/protocols\">protocol</a>\n was used. Libraries were run on an Illumina NextSeq2000 with\n paired-end sequencing (2x50 bp). Sample sequencing depth was\n 2.0 million reads (IgG) and 5.8 million reads (H3K27me3).\n Data were aligned to the hg19 genome using Bowtie2. Data\n were filtered to remove duplicates, multi-aligned reads, and\n blacklist regions.\n </span>\n </p>\n </div>\n </div>\n <aside class=\"image-picker__right\">\n <div class=\"image-picker__gallery\">\n <img\n loading=\"lazy\"\n alt=\"13-0048-cut-tag-data\"\n src=\"/content/images/products/antibodies/13-0048-cut-tag-data.jpeg\"\n width=\"200\"\n class=\"image-picker__side-image image-picker__side-image_active\"\n role=\"button\" />\n <img\n loading=\"lazy\"\n alt=\"cut-and-tag-methods\"\n src=\"/content/images/products/methods/cut-and-tag-methods.png\"\n width=\"200\"\n class=\"image-picker__side-image image-picker__side-image_active\"\n role=\"button\" />\n </div>\n </aside>\n </section>\n </div>\n </div>\n </div>\n <div id=\"prodAccordion\">\n <div id=\"ProductDescription\" class=\"Block Panel\">\n <h3 class=\"sub-title1\">Technical Information</h3>\n <div\n class=\"ProductDescriptionContainer product-droppdown__section-description\">\n <div class=\"product-tech-info\">\n <div class=\"product-tech-info__line-item\">\n <div class=\"product-tech-info__line-item-left\">\n <b>Immunogen</b>\n </div>\n <div class=\"product-tech-info__line-item-right\">\n Recombinant full-length mouse immunoglobulin protein\n </div>\n </div>\n <div class=\"product-tech-info__line-item\">\n <div class=\"product-tech-info__line-item-left\">\n <b>Storage</b>\n </div>\n <div class=\"product-tech-info__line-item-right\">\n Store at 4°C short term. For long term storage, store at\n -20°C. Avoid freeze/thaw cycles\n </div>\n </div>\n <div class=\"product-tech-info__line-item\">\n <div class=\"product-tech-info__line-item-left\">\n <b>Formulation</b>\n </div>\n <div class=\"product-tech-info__line-item-right\">\n Protein G affinity-purified antibody in PBS pH 7.2, 0.09% sodium\n azide\n </div>\n </div>\n </div>\n </div>\n </div>\n </div>\n <div id=\"prodAccordion\">\n <div id=\"ProductDescription\" class=\"Block Panel\">\n <h3 class=\"sub-title1\">Recommended Dilution</h3>\n <div\n class=\"ProductDescriptionContainer product-droppdown__section-description\">\n <div class=\"product-tech-info\">\n <div class=\"product-tech-info__line-item\">\n <div class=\"product-tech-info__line-item-left\">\n <b>CUT&Tag</b>\n </div>\n <div class=\"product-tech-info__line-item-right\">\n 0.5 µg per reaction\n </div>\n </div>\n </div>\n </div>\n </div>\n </div>\n <div id=\"prodAccordion\">\n <div id=\"ProductDescription\" class=\"Block Panel\">\n <h3 class=\"sub-title1\">References</h3>\n <div\n class=\"ProductDescriptionContainer product-droppdown__section-description\">\n <strong>Product References:</strong>\n <br />\n [1] Kaya-Okur et al. <em>Nat Commun</em> (2019). PMID:\n <a\n href=\"https://pubmed.ncbi.nlm.nih.gov/31036827/\"\n title=\"CUT&Tag for efficient epigenomic profiling of small samples and single cells\"\n target=\"_blank\"\n >31036827</a\n ><br />\n <br />\n <iframe\n class=\"airtable-embed\"\n src=\"https://airtable.com/embed/shrMDdIa64nYoOq1E?backgroundColor=purple&viewControls=on\"\n frameborder=\"0\"\n onmousewheel=\"\"\n width=\"100%\"\n height=\"300\"\n style=\"background: transparent; border: 1px solid #ccc\"></iframe>\n </div>\n </div>\n </div>\n <div id=\"prodAccordion\">\n <div id=\"ProductDescription\" class=\"Block Panel\">\n <h3 class=\"sub-title1\">Documents & Resources</h3>\n <div\n class=\"ProductDescriptionContainer product-droppdown__section-description\">\n <div class=\"product-documents\">\n <a\n href=\"/content/documents/tds/13-0048.pdf\"\n target=\"_blank\"\n class=\"product-documents__link\">\n <svg\n version=\"1.1\"\n id=\"Layer_1\"\n xmlns=\"http://www.w3.org/2000/svg\"\n 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M44.81,147.9c5.38,0,8.07-2.32,8.07-6.97c0-2.39-0.67-4.16-2-5.31\n c-1.33-1.15-3.36-1.73-6.07-1.73H34.59v14.01H44.81z\" />\n <path\n class=\"product-documents__svg-pdf\"\n d=\"M69.92,125.68h18.91c5.29,0,9.84,0.97,13.66,2.9c3.82,1.93,6.74,4.72,8.76,8.35\n c2.02,3.63,3.04,7.98,3.04,13.04c0,5.06-1,9.42-3,13.08c-2,3.66-4.91,6.45-8.73,8.38c-3.82,1.93-8.4,2.9-13.73,2.9H69.92V125.68z\n M88.07,165.63c10.35,0,15.52-5.22,15.52-15.66c0-10.4-5.17-15.59-15.52-15.59h-7.38v31.26H88.07z\" />\n <path\n class=\"product-documents__svg-pdf\"\n d=\"M122.57,125.68h32.84v8.49h-22.22v11.18h20.84v8.49h-20.84v20.49h-10.63V125.68z\" />\n </g>\n </svg>\n <span class=\"product-documents__info\">Technical Datasheet</span>\n </a>\n </div>\n </div>\n </div>\n </div>\n </div>\n</div>\n","tags":[],"warranty":"","price":{"without_tax":{"formatted":"$85.00","value":85,"currency":"USD"},"tax_label":"Sales Tax"},"detail_messages":"","availability":"","page_title":"Anti-Mouse Secondary Antibody for CUTANA™ CUT&Tag Workflows","cart_url":"https://www.epicypher.com/cart.php","max_purchase_quantity":0,"mpn":"","upc":null,"options":[{"id":1126,"type":"Configurable_PickList_Set","display_name":"Select Pack Size:","required":true,"condition":true,"state":"variant_option","values":[{"label":"50 Reactions","id":129,"data":"50 Reactions","selected":true},{"label":"250 Reactions","id":130,"data":"250 Reactions","selected":false}],"partial":"set-radio"}],"related_products":[{"id":764,"sku":null,"name":"CUTANA™ pAG-Tn5 for CUT&Tag","url":"https://www.epicypher.com/products/epigenetics-kits-and-reagents/cutana-pag-tn5-for-cut-and-tag","availability":"","rating":null,"brand":{"name":null},"category":["Epigenetics Kits and Reagents","Epigenetics Kits and Reagents/CUTANA™ CUT&Tag Assays"],"summary":"\n \n \n Type: Transposase\n \n \n Mol Wgt: 191 kDa\n \n \n \n ","image":{"data":"https://cdn11.bigcommerce.com/s-y9o92/images/stencil/{:size}/products/764/750/2020_CUTTag_icon_RGB_Andy_L__93238.1592491196.png?c=2","alt":"CUTANA™ pAG-Tn5 for CUT&Tag"},"images":[{"data":"https://cdn11.bigcommerce.com/s-y9o92/images/stencil/{:size}/products/764/750/2020_CUTTag_icon_RGB_Andy_L__93238.1592491196.png?c=2","alt":"CUTANA™ pAG-Tn5 for CUT&Tag"}],"date_added":"17th Jun 2020","pre_order":false,"show_cart_action":true,"has_options":true,"stock_level":null,"low_stock_level":null,"qty_in_cart":0,"custom_fields":[{"id":1005,"name":"Internal Comment","value":"extra boxes in bottom shelf of Venom"}],"num_reviews":null,"weight":{"formatted":"0.01 LBS","value":0.01},"demo":false,"price":{"without_tax":{"currency":"USD","formatted":"$765.00","value":765},"price_range":{"min":{"without_tax":{"currency":"USD","formatted":"$765.00","value":765},"tax_label":"Sales Tax"},"max":{"without_tax":{"currency":"USD","formatted":"$2,970.00","value":2970},"tax_label":"Sales 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