Anti-Mouse

Anti-Mouse Secondary Antibody for CUTANA™ CUT&Tag Workflows

$75.00
SKU: 13-0048
Select Pack Size: *
  • Type:  Mixed Monoclonal*
  • Host:  Goat
  • Applications:  CUT&Tag
  • Reactivity:  Anti-Mouse
  • Format:  Affinity Purified IgG
  • Target Size:  N/A

Description

Anti-Mouse Secondary Antibody for CUTANA™ CUT&Tag is affinity-purified and specific for mouse immunoglobulins. Minimal cross reactivity with rabbit, rat, human, bovine, guinea pig, and donkey IgG is observed. Use of a secondary antibody in CUT&Tag aids in signal amplification by increasing bound IgG per target epitope. For best results, use with CUTANA™ pAG-Tn5 (EpiCypher 15-1017 / 15-1117).

*Mixed Monoclonal: a pool of multiple monoclonal antibodies.

Validation Data

Figure 1: CUT&Tag data
CUT&Tag was performed on 100k nuclei with mouse anti-H3K27me3 antibody and 0.5 µg of anti-mouse secondary antibody for CUTANA™ CUT&Tag using the EpiCypher CUTANA™ Direct-to-PCR CUT&Tag protocol. Gene browser shots generated using the Integrative Genomics Viewer (IGV, Broad Institute) show a representative 324 kb window centered at the LAMC3 gene. The genomic distribution pattern was consistent with that of H3K27me3.

Technical Information

Immunogen
Recombinant full-length mouse immunoglobulin protein.
Formulation
Protein G affinity-purified antibody in PBS pH 7.2, with 0.09% sodium azide
Storage and Stability
Store at 4°C short term. For long term storage, store at -20°C. Avoid freeze/thaw cycles.

Recommended Dilution

CUT&Tag: 0.5 µg per reaction

References

Product References:

Documents & Resources

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