CUTANA™ Uncharged pAG-Tn5 for CUT&Tag
78 in stock
Powered by BiozProducts in EpiCypher’s IDEA Toolbox (Innovation and Discovery of Epigenetic Applications) offer access to reagents without known or fully defined uses, enabling researchers to explore cutting-edge applications. Due to their novelty and unexplored potential, EpiCypher will engage in limited technical support.
CUTANA Uncharged pAG-Tn5 is a fusion of Proteins A and G to E. coli transposase (Tn5) and is the key enzyme for CUT&Tag [1]. This product is highly purified to remove contaminating E. coli DNA. For superior normalization, as well as antibody validation and reaction monitoring, SNAP-CUTANA™ nucleosome spike-ins (e.g. EpiCypher 19-1002) are recommended. This Tn5 is uncharged and must be loaded with user-designed mosaic adapter DNA prior to use in CUT&Tag. For a charged enzyme that is ready to use, see EpiCypher 15-1017.
Intellectual Property
Background References:
[1] Kaya-Okur et al. Nat. Commun. (2019). PMID: 31036827
Figure 1: Protein gel data
- Type: Transposase
- Mol Wgt: 157 kDa (dimer)
- Host: E. coli
- Epitope Tag: None
Formulation
50 mM HEPES-KOH pH 7.2, 100 mM NaCl, 0.1 mM EDTA, 1 mM DTT, 0.1% Triton X-100, 50% glycerol
Application Notes
pAG-Tn5 transposomes can be assembled as previously described [1]. In brief, 3.2 µL of a 100 µM equimolar mixture of pre-annealed user-defined Adapter-A and user-defined Adapter-B oligonucleotides should be mixed with 20 µL of 5.5 µM pAG-Tn5 fusion protein dimer. The mixture is then incubated on a rotating platform for 1 hour at room temperature and stored at -20°C. Specific activity definition of the charged pAG-Tn5 is highly recommended before use in CUT&Tag. Due to the confounding variable of user-supplied mosaic adapters, EpiCypher will not engage in protocol troubleshooting for this reagent. For a pre-charged enzyme that is eligible for technical support, see EpiCypher 15-1017.
| Item | Cat. No. |
|---|---|
| CUTANA™ Concanavalin A Conjugated Paramagnetic Beads | 21-1401 |
| CUTANA™ Rabbit IgG CUT&RUN Negative Control Antibody | 13-0042 |
| Histone H3K4me3 Antibody, SNAP-Certified™ for CUT&RUN and ChIP | 13-0041 |
| Anti-Rabbit Secondary Antibody for CUTANA™ CUT&Tag | 13-0047 |
| Anti-Mouse Secondary Antibody for CUTANA™ CUT&Tag | 13-0048 |
| SNAP-CUTANA™ K-MetStat Panel | 19-1002 |
| CUTANA™ Non-Hot Start 2X PCR Master Mix for CUT&Tag | 15-1018 |
| Magnetic Separation Rack, 0.2 mL Tubes | 10-0008 |
| Magnetic Separation Rack, 1.5 mL Tubes | 10-0012 |
| CUTANA™ CUT&RUN 8-strip 0.2 mL Tubes | 10-0009 |
