

CUTANA™ ChIC/CUT&RUN Kit
{"url":"https://www.epicypher.com/products/epigenetics-reagents-and-assays/cutana-chic-cut-and-run-kit","add_this":[{"service":"facebook","annotation":""},{"service":"email","annotation":""},{"service":"print","annotation":""},{"service":"twitter","annotation":""},{"service":"linkedin","annotation":""}],"gtin":null,"id":"772","bulk_discount_rates":[],"can_purchase":true,"meta_description":"CUTANA™ ChIC/CUT&RUN Kit includes all needed reagents to go from cells to purified CUT&RUN DNA. ","category":["Epigenetics Kits and Reagents","Epigenetics Kits and Reagents/CUTANA™ ChIC / CUT&RUN Assays"],"AddThisServiceButtonMeta":"","main_image":{"data":"https://cdn11.bigcommerce.com/s-y9o92/images/stencil/{:size}/products/772/998/Epicypher_CUTANA_CUTRUN_Box__31908.1646406725.png?c=2","alt":"CUTANA™ ChIC/CUT&RUN Kit"},"add_to_wishlist_url":"/wishlist.php?action=add&product_id=772","shipping":{"calculated":true},"num_reviews":0,"weight":"0.01 LBS","custom_fields":[{"id":"588","name":"Pack size","value":"48 Reactions"}],"sku":"14-1048","description":"<div class=\"service_accordion product-droppdown\">\n <div class=\"container\">\n <div id=\"prodAccordion\">\n <div id=\"ProductDescription\" class=\"Block Panel current\">\n <h3 class=\"sub-title1\">Description</h3>\n <div\n class=\"ProductDescriptionContainer product-droppdown__section-description-specific\">\n <p>\n The CUTANA™ ChIC/CUT&RUN Kit enables streamlined chromatin profiling\n of histone post-translational modifications (PTMs) and chromatin\n associated proteins while providing increased throughput and\n reproducibility with multi-channel pipetting.\n </p>\n <p\n style=\"\n background-color: #4698cb;\n color: #fff;\n padding: 1.3rem;\n text-align: center;\n border-radius: 12px;\n \">\n <a\n target=\"_blank\"\n style=\"color: #fff\"\n href=\"/products/epigenetics-reagents-and-assays/cutana-cut-run-library-prep-kit\"\n >Save 10% when you buy with our Library Prep Kit for a streamlined\n CUT&RUN workflow! Learn more >>\n </a>\n </p>\n <p>\n Positive (H3K4me3) and negative (IgG) control antibodies are\n included to pair with SNAP-CUTANA™ spike-in controls for assay\n optimization and continuous monitoring (<strong>Figure 2</strong>).\n <em>E. coli</em> DNA is included for data normalization. The kit is\n compatible with a variety of inputs including cells or nuclei\n derived from native, cryopreserved, or cross-linked samples. While\n it is recommended to start with 500,000 cells, comparable data can\n be generated using as few as 5,000 cells. The inclusion of controls\n and compatibility with diverse target types, sample inputs, and low\n cell numbers make this kit the go-to solution for chromatin mapping\n experiments. To place bulk orders, contact\n <a href=\"mailto:info@epicypher.com\">info@epicypher.com</a>.\n </p>\n </div>\n </div>\n </div>\n <div id=\"prodAccordion\">\n <div id=\"ProductDescription\" class=\"Block Panel current\">\n <h3 class=\"sub-title1\">Validation Data</h3>\n <div\n class=\"ProductDescriptionContainer product-droppdown__section-description-specific\">\n <section class=\"image-picker\">\n <div class=\"image-picker__left\">\n <div\n class=\"image-picker__main-content_active image-picker__main-content\">\n <div class=\"image-picker__header-content\">\n <button class=\"image-picker__left-arrow\">\n <svg\n class=\"image-picker__svg-left\"\n width=\"24\"\n height=\"24\"\n viewBox=\"0 0 24 24\">\n <path\n d=\"M16.67 0l2.83 2.829-9.339 9.175 9.339 9.167-2.83 2.829-12.17-11.996z\" />\n </svg>\n </button>\n <a\n href=\"/content/images/products/kits/14-1048-DFSDA.jpg\"\n target=\"_new\"\n class=\"image-picker__main-image-link\"\n ><img\n loading=\"lazy\"\n alt=\"14-1048-DFSDA\"\n src=\"/content/images/products/kits/14-1048-DFSDA.jpg\"\n loading=\"lazy\"\n class=\"image-picker__main-image\" />\n <span class=\"image-picker__main-image-caption\"\n >(Click to enlarge)</span\n ></a\n >\n <button class=\"image-picker__right-arrow\">\n <svg\n class=\"image-picker__svg-right\"\n width=\"24\"\n height=\"24\"\n viewBox=\"0 0 24 24\">\n <path\n d=\"M7.33 24l-2.83-2.829 9.339-9.175-9.339-9.167 2.83-2.829 12.17 11.996z\" />\n </svg>\n </button>\n </div>\n <p>\n <span class=\"image-picker__span-content\"\n ><strong\n >Figure 1: CUT&RUN DNA Fragment Size Distribution Analysis </strong\n ><br />\n CUT&RUN was performed using the CUTANA ChIC/CUT&RUN Kit\n starting with 500,000 K-562 cells. CUT&RUN DNA, isolated\n from IgG Negative Control (EpiCypher\n <a\n href=\"/products/nucleosomes/snap-cutana-spike-in-controls/cutana-rabbit-igg-cut-run-negative-control-antibody\"\n >13-0042</a\n >k) and H3K4me3 Positive Control (EpiCypher\n <a\n href=\"/products/antibodies/snap-chip-certified-antibodies/histone-h3k4me3-antibody-snap-chip-certified-cutana-cut-run-compatible\">\n 13-0041</a\n >k) antibodies, was used to prepare paired-end Illumina\n sequencing libraries. Library DNA was analyzed by Agilent\n Tapestation<sup>®</sup>. This analysis confirmed that\n mononucleosomes were predominantly enriched in CUT&RUN (~300\n bp peak represents 150 bp nucleosomes + sequencing\n adapters).\n </span>\n </p>\n </div>\n <div class=\"image-picker__main-content\">\n <div class=\"image-picker__header-content\">\n <button class=\"image-picker__left-arrow\">\n <svg\n class=\"image-picker__svg-left\"\n width=\"24\"\n height=\"24\"\n viewBox=\"0 0 24 24\">\n <path\n d=\"M16.67 0l2.83 2.829-9.339 9.175 9.339 9.167-2.83 2.829-12.17-11.996z\" />\n </svg>\n </button>\n <a\n href=\"/content/images/products/kits/14-1048-k-metstat-spike-in-controls.jpeg\"\n target=\"_new\"\n class=\"image-picker__main-image-link\"\n ><img\n loading=\"lazy\"\n alt=\"14-1048-k-metstat-spike-in-controls\"\n src=\"/content/images/products/kits/14-1048-k-metstat-spike-in-controls.jpeg\"\n class=\"image-picker__main-image\"\n loading=\"lazy\" />\n <span class=\"image-picker__main-image-caption\"\n >(Click to enlarge)</span\n ></a\n >\n <button class=\"image-picker__right-arrow\">\n <svg\n class=\"image-picker__svg-right\"\n width=\"24\"\n height=\"24\"\n viewBox=\"0 0 24 24\">\n <path\n d=\"M7.33 24l-2.83-2.829 9.339-9.175-9.339-9.167 2.83-2.829 12.17 11.996z\" />\n </svg>\n </button>\n </div>\n <p>\n <span class=\"image-picker__span-content\"\n ><strong\n >Figure 2: SNAP-CUTANA™ K-MetStat Spike-in Controls </strong\n ><br />\n DNA-barcoded designer nucleosomes (dNucs) representing 16\n different K-methyl PTM states: mono-, di-, and\n tri-methylation at H3K4, H3K9, H3K27, H3K36, and H4K20, as\n well as unmodified control, were spiked into CUT&RUN samples\n prior to the addition of the control antibodies provided\n with the kit (IgG, H3K4me3). Instances of each spike-in\n barcode were counted and normalized from raw fastq files\n using the shell script and analysis excel sheet available on\n the spike-in product page (EpiCypher\n <a href=\"/products/nucleosomes/snap-cutana-k-metstat-panel\"\n >19-1002</a\n >). Barcodes for IgG (top; normalized to the sum of total\n reads) and H3K4me3 (bottom; normalized to on-target)\n antibodies provided with this kit are shown. The spike-ins\n confirmed optimal experimental conditions (H3K4me3 antibody\n specifically recovered the target dNuc, while IgG showed no\n preferential enrichment).\n </span>\n </p>\n </div>\n <div class=\"image-picker__main-content\">\n <div class=\"image-picker__header-content\">\n <button class=\"image-picker__left-arrow\">\n <svg\n class=\"image-picker__svg-left\"\n width=\"24\"\n height=\"24\"\n viewBox=\"0 0 24 24\">\n <path\n d=\"M16.67 0l2.83 2.829-9.339 9.175 9.339 9.167-2.83 2.829-12.17-11.996z\" />\n </svg>\n </button>\n <a\n href=\"/content/images/products/kits/14-1048-CRGWH.jpg\"\n target=\"_new\"\n class=\"image-picker__main-image-link\">\n <img\n loading=\"lazy\"\n alt=\"14-1048-CRGWH\"\n src=\"/content/images/products/kits/14-1048-CRGWH.jpg\"\n loading=\"lazy\"\n class=\"image-picker__main-image\" />\n <span class=\"image-picker__main-image-caption\"\n >(Click to enlarge)</span\n >\n </a>\n <button class=\"image-picker__right-arrow\">\n <svg\n class=\"image-picker__svg-right\"\n width=\"24\"\n height=\"24\"\n viewBox=\"0 0 24 24\">\n <path\n d=\"M7.33 24l-2.83-2.829 9.339-9.175-9.339-9.167 2.83-2.829 12.17 11.996z\" />\n </svg>\n </button>\n </div>\n <p>\n <span class=\"image-picker__span-content\">\n <strong>Figure 3: CUT&RUN genome-wide heatmaps </strong\n ><br />\n CUT&RUN data was generated using the CUTANA ChIC/CUT&RUN Kit\n with 500,000 K-562 cells. Three replicates (“Rep”) of IgG\n (left) and H3K4me3 (right) antibodies are shown in a\n heatmap. CUT&RUN signal (from an average of 5.9 million\n paired-end reads) aligned to the transcription start site\n (TSS, +/- 2kb) are presented for 18,793 genes. High and low\n signal are ranked by intensity (top to bottom) and reflected\n by red and blue colors, respectively. Gene rows in each\n heatmap are aligned and sorted from high to low signal\n relative to H3K4me3 Rep 3 (far right), demonstrating the\n reproducibility of the kit workflow.\n </span>\n </p>\n </div>\n <div class=\"image-picker__main-content\">\n <div class=\"image-picker__header-content\">\n <button class=\"image-picker__left-arrow\">\n <svg\n class=\"image-picker__svg-left\"\n width=\"24\"\n height=\"24\"\n viewBox=\"0 0 24 24\">\n <path\n d=\"M16.67 0l2.83 2.829-9.339 9.175 9.339 9.167-2.83 2.829-12.17-11.996z\" />\n </svg>\n </button>\n <a\n href=\"/content/images/products/kits/14-1048-RGBT.jpg\"\n target=\"_new\"\n class=\"image-picker__main-image-link\">\n <img\n loading=\"lazy\"\n alt=\"14-1048-RGBT\"\n src=\"/content/images/products/kits/14-1048-RGBT.jpg\"\n loading=\"lazy\"\n class=\"image-picker__main-image\" />\n <span class=\"image-picker__main-image-caption\"\n >(Click to enlarge)</span\n >\n </a>\n <button class=\"image-picker__right-arrow\">\n <svg\n class=\"image-picker__svg-right\"\n width=\"24\"\n height=\"24\"\n viewBox=\"0 0 24 24\">\n <path\n d=\"M7.33 24l-2.83-2.829 9.339-9.175-9.339-9.167 2.83-2.829 12.17 11.996z\" />\n </svg>\n </button>\n </div>\n <p>\n <span class=\"image-picker__span-content\">\n <strong\n >Figure 4: Representative gene browser tracks </strong\n ><br />\n CUT&RUN data was generated as described in Figure 3. A\n representative 150 kb window at the TRMT2A gene is shown for\n three replicates (“Rep”) of IgG and H3K4me3 antibody\n controls (included in the kit). Representative tracks are\n also shown for H3K27me3 (EpiCypher 13-0030) and the\n transcription factor CTCF (EMD Millipore 07-729) antibodies.\n The CUT&RUN kit produced the expected genomic distribution\n for each target. Images were generated using the Integrative\n Genomics Viewer (IGV, Broad Institute).\n </span>\n </p>\n </div>\n </div>\n <aside class=\"image-picker__right\">\n <div class=\"image-picker__gallery\">\n <img\n loading=\"lazy\"\n alt=\"14-1048-DFSDA\"\n src=\"/content/images/products/kits/14-1048-DFSDA.jpg\"\n width=\"200\"\n loading=\"lazy\"\n class=\"image-picker__side-image image-picker__side-image_active\"\n role=\"button\" />\n <img\n loading=\"lazy\"\n alt=\"14-1048-k-metstat-spike-in-controls\"\n src=\"/content/images/products/kits/14-1048-k-metstat-spike-in-controls.jpeg\"\n class=\"image-picker__side-image\"\n loading=\"lazy\"\n role=\"button\" />\n <img\n loading=\"lazy\"\n alt=\"14-1048-CRGWH\"\n src=\"/content/images/products/kits/14-1048-CRGWH.jpg\"\n loading=\"lazy\"\n class=\"image-picker__side-image\"\n role=\"button\" />\n <img\n loading=\"lazy\"\n alt=\"14-1048-RGBT\"\n src=\"/content/images/products/kits/14-1048-RGBT.jpg\"\n loading=\"lazy\"\n class=\"image-picker__side-image\"\n role=\"button\" />\n </div>\n </aside>\n </section>\n </div>\n </div>\n </div>\n\n <div id=\"prodAccordion\">\n <div id=\"ProductDescription\" class=\"Block Panel\">\n <h3 class=\"sub-title1\">Kit Contents</h3>\n <div\n class=\"ProductDescriptionContainer product-droppdown__section-description\">\n <table style=\"color: #525051\">\n <tr>\n <th\n style=\"\n padding: 0;\n text-align: left;\n color: #525051;\n text-transform: none;\n \">\n <u>Item</u>\n </th>\n <th style=\"padding: 0; text-align: left; color: #525051\">\n <u style=\"text-transform: none\">Cat. No.</u>\n </th>\n </tr>\n\n <tr>\n <td>CUTANA™ pAG-MNase for ChIC/CUT&RUN Workflows</td>\n <td>\n <a\n href=\"/products/epigenetics-reagents-and-assays/cutana-pag-mnase-for-chic-cut-and-run-workflows\"\n >15-1016</a\n >\n </td>\n </tr>\n\n <tr>\n <td>\n Histone H3K4me3 Antibody, SNAP-Certified™ for CUT&RUN and ChIP\n </td>\n <td>\n <a\n href=\"/products/antibodies/snap-chip-certified-antibodies/histone-h3k4me3-antibody-snap-chip-certified-cutana-cut-run-compatible\"\n >13-0041</a\n >k\n </td>\n </tr>\n\n <tr>\n <td>CUTANA™ Rabbit IgG CUT&RUN Negative Control Antibody</td>\n <td>\n <a\n href=\"/products/nucleosomes/snap-cutana-spike-in-controls/cutana-rabbit-igg-cut-run-negative-control-antibody\"\n >13-0042</a\n >k\n </td>\n </tr>\n\n <tr>\n <td>SNAP-CUTANA™ K-MetStat Panel</td>\n <td>\n <a href=\"/products/nucleosomes/snap-cutana-k-metstat-panel\"\n >19-1002</a\n >k\n </td>\n </tr>\n\n <tr>\n <td>CUTANA™ Concanavalin A Conjugated Paramagnetic Beads</td>\n <td>\n <a\n href=\"/products/epigenetics-kits-and-reagents/cutana-chic-cut-run-assays/cutana-concanavalin-a-conjugated-paramagnetic-beads\"\n >21-1401</a\n >\n </td>\n </tr>\n\n <tr>\n <td>CUTANA™ <em>E. coli</em> Spike-in DNA</td>\n <td>\n <a\n href=\"/products/nucleosomes/snap-cutana-spike-in-controls/cutana-e-coli-spike-in-dna\"\n >18-1401</a\n >\n </td>\n </tr>\n\n <tr>\n <td>CUTANA™ CUT&RUN 8-strip 0.2 mL Tubes</td>\n <td>\n <a\n href=\"/products/epigenetics-reagents-and-assays/cutana-chic-cut-run-assays/cutana-cut-run-8-strip-0-2-ml-tubes\"\n >10-0009</a\n >\n </td>\n </tr>\n\n <tr>\n <td>Bead Activation Buffer</td>\n <td>21-1001</td>\n </tr>\n\n <tr>\n <td>Pre-Wash Buffer</td>\n <td>21-1002</td>\n </tr>\n\n <tr>\n <td>Stop Buffer</td>\n <td>21-1003</td>\n </tr>\n\n <tr>\n <td>5% Digitonin</td>\n <td>21-1004</td>\n </tr>\n\n <tr>\n <td>1 M Spermidine</td>\n <td>21-1005</td>\n </tr>\n\n <tr>\n <td>0.5 M EDTA</td>\n <td>21-1006</td>\n </tr>\n\n <tr>\n <td>100 mM Calcium Chloride</td>\n <td>21-1007</td>\n </tr>\n\n <tr>\n <td>DNA Cleanup Columns</td>\n <td>10-0010</td>\n </tr>\n\n <tr>\n <td>DNA Collection Tubes</td>\n <td>10-0011</td>\n </tr>\n\n <tr>\n <td>DNA Binding Buffer</td>\n <td>21-1008</td>\n </tr>\n\n <tr>\n <td>DNA Wash Buffer</td>\n <td>21-1009</td>\n </tr>\n\n <tr>\n <td>DNA Elution Buffer</td>\n <td>21-1010</td>\n </tr>\n </table>\n </div>\n </div>\n </div>\n\n <div id=\"prodAccordion\">\n <div id=\"ProductDescription\" class=\"Block Panel\">\n <h3 class=\"sub-title1\">Recommended Accessory Products</h3>\n <div\n class=\"ProductDescriptionContainer product-droppdown__section-description\">\n <table style=\"color: #525051\" id=\"specific-table\">\n <tr>\n <th\n style=\"\n padding: 0;\n text-align: left;\n color: #525051;\n text-transform: none;\n \">\n <u>Item</u>\n </th>\n <th style=\"padding: 0; text-align: left; color: #525051\">\n <u style=\"text-transform: none\">Cat No.</u>\n </th>\n </tr>\n\n <tr>\n <td>CUTANA™ CUT&RUN Library Prep Kit</td>\n <td>\n <a\n href=\"/products/epigenetics-reagents-and-assays/cutana-cut-and-run-library-prep-kit\"\n >14-1001/14-1002</a\n >\n </td>\n </tr>\n\n <tr>\n <td>SNAP-CUTANA™ K-MetStat Panel</td>\n <td>\n <a href=\"/products/nucleosomes/snap-cutana-k-metstat-panel\"\n >19-1002</a\n >\n </td>\n </tr>\n\n <tr>\n <td id=\"specific-row\">CUT&RUN Antibodies</td>\n <td>\n <a href=\"/products/antibodies/cutana-cut-and-run-antibodies\"\n >See the list</a\n >\n </td>\n </tr>\n\n <tr>\n <td id=\"specific-row\">Magnetic Separation Rack, 0.2 mL Tubes</td>\n <td>\n <a\n href=\"/products/epigenetics-reagents-and-assays/cutana-chic-cut-run-assays/magnetic-separation-rack-0-2-ml-tubes\"\n >10-0008</a\n >\n </td>\n </tr>\n\n <tr>\n <td id=\"specific-row\">Magnetic Separation Rack, 1.5 mL Tubes</td>\n <td>\n <a\n href=\"/products/epigenetics-reagents-and-assays/magnetic-separation-rack-1-5-ml-tubes\"\n >10-0012</a\n >\n </td>\n </tr>\n </table>\n </div>\n </div>\n </div>\n\n <div id=\"prodAccordion\">\n <div id=\"ProductDescription\" class=\"Block Panel\">\n <h3 class=\"sub-title1\">Technical Information</h3>\n <div\n class=\"ProductDescriptionContainer product-droppdown__section-description\">\n <div class=\"product-tech-info\">\n <div class=\"product-tech-info__line-item\">\n <div class=\"product-tech-info__line-item-left\">\n <b>Kit Contents</b>\n </div>\n <div class=\"product-tech-info__line-item-right\">\n Kit contains buffers, enzymes, magnetic beads, control\n antibodies, spike-in controls, 8-strip tubes, and spin columns\n necessary to prepare and purify CUT&RUN DNA starting from cells\n or nuclei. See Kit Manual for additional materials and equipment\n required for the protocol.\n </div>\n </div>\n <div class=\"product-tech-info__line-item\">\n <div class=\"product-tech-info__line-item-left\">\n <b>Storage and Stability</b>\n </div>\n <div class=\"product-tech-info__line-item-right\">\n OPEN KIT IMMEDIATELY and store components at room temperature,\n 4°C, and -20°C as indicated (see Kit Manual for full\n instructions). Stable for 6 months upon date of receipt.\n </div>\n </div>\n <div class=\"product-tech-info__line-item\">\n <div class=\"product-tech-info__line-item-left\">\n <b>Instructions for Use</b>\n </div>\n <div class=\"product-tech-info__line-item-right\">\n See Kit Manual for complete instructions.\n </div>\n </div>\n </div>\n </div>\n </div>\n </div>\n\n <h3\n style=\"color: #4698cb; font-size: 24px; border-bottom: none\"\n class=\"custom-title-description\">\n Documents & Resources\n </h3>\n\n <div id=\"prodAccordion\">\n <div id=\"ProductDescription\" class=\"Block Panel\">\n <!-- <h3 style=\"color: #4698cb; margin-bottom: 0\">Current Lot</h3> -->\n <h3\n style=\"padding-bottom: 5px; margin-top: 1rem; padding-top: 0\"\n class=\"sub-title1\">\n Kit Version 3\n </h3>\n <div\n class=\"ProductDescriptionContainer product-droppdown__section-description\">\n <div class=\"product-documents\">\n <a\n href=\"/content/documents/tds/14-1048.pdf\"\n target=\"_new\"\n class=\"product-documents__link\">\n <svg\n version=\"1.1\"\n id=\"Layer_1\"\n xmlns=\"http://www.w3.org/2000/svg\"\n xmlns:xlink=\"http://www.w3.org/1999/xlink\"\n x=\"0px\"\n y=\"0px\"\n viewBox=\"0 0 228 240\"\n style=\"enable-background: new 0 0 228 240\"\n xml:space=\"preserve\"\n class=\"product-documents__icon\"\n alt=\"16-0002 Datasheet\">\n <g>\n <path\n class=\"product-documents__svg-pdf\"\n d=\"M191.92,68.77l-47.69-47.69c-1.33-1.33-3.12-2.08-5.01-2.08H45.09C41.17,19,38,22.17,38,26.09v184.36\n c0,3.92,3.17,7.09,7.09,7.09h141.82c3.92,0,7.09-3.17,7.09-7.09V73.8C194,71.92,193.25,70.1,191.92,68.77z M177.65,77.06h-41.7\n v-41.7L177.65,77.06z M178.05,201.59H53.95V34.95h66.92v47.86c0,5.14,4.17,9.31,9.31,9.31h47.86V201.59z\" />\n </g>\n <rect\n x=\"20\"\n y=\"112\"\n class=\"product-documents__svg-background\"\n width=\"146\"\n height=\"76\" />\n <g>\n <path\n class=\"product-documents__svg-pdf\"\n d=\"M23.83,125.68h22.36c5.29,0,9.41,1.33,12.35,4c2.94,2.67,4.42,6.39,4.42,11.18c0,4.78-1.47,8.51-4.42,11.18\n c-2.94,2.67-7.06,4-12.35,4H34.59v18.29H23.83V125.68z M44.81,147.9c5.38,0,8.07-2.32,8.07-6.97c0-2.39-0.67-4.16-2-5.31\n c-1.33-1.15-3.36-1.73-6.07-1.73H34.59v14.01H44.81z\" />\n <path\n class=\"product-documents__svg-pdf\"\n d=\"M69.92,125.68h18.91c5.29,0,9.84,0.97,13.66,2.9c3.82,1.93,6.74,4.72,8.76,8.35\n c2.02,3.63,3.04,7.98,3.04,13.04c0,5.06-1,9.42-3,13.08c-2,3.66-4.91,6.45-8.73,8.38c-3.82,1.93-8.4,2.9-13.73,2.9H69.92V125.68z\n M88.07,165.63c10.35,0,15.52-5.22,15.52-15.66c0-10.4-5.17-15.59-15.52-15.59h-7.38v31.26H88.07z\" />\n <path\n class=\"product-documents__svg-pdf\"\n d=\"M122.57,125.68h32.84v8.49h-22.22v11.18h20.84v8.49h-20.84v20.49h-10.63V125.68z\" />\n </g>\n </svg>\n <span class=\"product-documents__info\">Technical Datasheet </span>\n </a>\n </div>\n <div class=\"product-documents\">\n <a\n href=\"/content/documents/manuals/cut-and-run-kit-manual-v3.pdf\"\n target=\"_new\"\n class=\"product-documents__link\">\n <svg\n version=\"1.1\"\n id=\"Layer_1\"\n xmlns=\"http://www.w3.org/2000/svg\"\n xmlns:xlink=\"http://www.w3.org/1999/xlink\"\n x=\"0px\"\n y=\"0px\"\n viewBox=\"0 0 228 240\"\n style=\"enable-background: new 0 0 228 240\"\n xml:space=\"preserve\"\n class=\"product-documents__icon\"\n alt=\"16-0002 Datasheet\">\n <g>\n <path\n class=\"product-documents__svg-pdf\"\n d=\"M191.92,68.77l-47.69-47.69c-1.33-1.33-3.12-2.08-5.01-2.08H45.09C41.17,19,38,22.17,38,26.09v184.36\n c0,3.92,3.17,7.09,7.09,7.09h141.82c3.92,0,7.09-3.17,7.09-7.09V73.8C194,71.92,193.25,70.1,191.92,68.77z M177.65,77.06h-41.7\n v-41.7L177.65,77.06z M178.05,201.59H53.95V34.95h66.92v47.86c0,5.14,4.17,9.31,9.31,9.31h47.86V201.59z\" />\n </g>\n <rect\n x=\"20\"\n y=\"112\"\n class=\"product-documents__svg-background\"\n width=\"146\"\n height=\"76\" />\n <g>\n <path\n class=\"product-documents__svg-pdf\"\n 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target=\"_new\"\n class=\"product-documents__link\">\n <svg\n version=\"1.1\"\n id=\"Layer_1\"\n xmlns=\"http://www.w3.org/2000/svg\"\n xmlns:xlink=\"http://www.w3.org/1999/xlink\"\n x=\"0px\"\n y=\"0px\"\n viewBox=\"0 0 228 240\"\n style=\"enable-background: new 0 0 228 240\"\n xml:space=\"preserve\"\n class=\"product-documents__icon\"\n alt=\"16-0002 Datasheet\">\n <g>\n <path\n class=\"product-documents__svg-pdf\"\n d=\"M191.92,68.77l-47.69-47.69c-1.33-1.33-3.12-2.08-5.01-2.08H45.09C41.17,19,38,22.17,38,26.09v184.36\n c0,3.92,3.17,7.09,7.09,7.09h141.82c3.92,0,7.09-3.17,7.09-7.09V73.8C194,71.92,193.25,70.1,191.92,68.77z M177.65,77.06h-41.7\n v-41.7L177.65,77.06z M178.05,201.59H53.95V34.95h66.92v47.86c0,5.14,4.17,9.31,9.31,9.31h47.86V201.59z\" />\n </g>\n <rect\n x=\"20\"\n y=\"112\"\n class=\"product-documents__svg-background\"\n width=\"146\"\n height=\"76\" />\n <g>\n <path\n class=\"product-documents__svg-pdf\"\n 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href=\"/content/documents/SNAP-CUTANA_K-MetStat_Panel_ShellScript.sh\"\n target=\"_blank\"\n class=\"product-documents__link\">\n <svg\n version=\"1.1\"\n id=\"Layer_1\"\n xmlns=\"http://www.w3.org/2000/svg\"\n xmlns:xlink=\"http://www.w3.org/1999/xlink\"\n x=\"0px\"\n y=\"0px\"\n viewBox=\"0 0 228 240\"\n style=\"enable-background: new 0 0 228 240\"\n xml:space=\"preserve\"\n class=\"product-documents__icon\">\n <g>\n <path\n class=\"product-documents__svg-txt\"\n d=\"M199.12,66.39l-51.16-51.16c-1.43-1.43-3.35-2.23-5.37-2.23H41.61c-4.2,0-7.61,3.41-7.61,7.61v197.79\n c0,4.2,3.41,7.61,7.61,7.61h152.14c4.2,0,7.61-3.41,7.61-7.61V71.79C201.36,69.77,200.55,67.82,199.12,66.39L199.12,66.39z\n M183.81,75.28h-44.74V30.54L183.81,75.28z M184.24,208.88H51.12V30.12h71.79v51.35c0,5.51,4.47,9.98,9.98,9.98h51.35V208.88z\n M115.78,144.7H72.04c-1.05,0-1.9,0.86-1.9,1.9v11.41c0,1.05,0.86,1.9,1.9,1.9h43.74c1.05,0,1.9-0.86,1.9-1.9V146.6\n C117.68,145.55,116.82,144.7,115.78,144.7z 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#4698cb; margin-bottom: 0; margin-top: 3rem\">\n Previous Lots\n </h3> -->\n <h3 style=\"padding-bottom: 5px; margin-top: 1rem\" class=\"sub-title1\">\n Kit Version 2\n </h3>\n <div\n class=\"ProductDescriptionContainer product-droppdown__section-description\">\n <div class=\"product-documents\">\n <a\n href=\"/content/documents/tds/14-1048-21130003-01.pdf\"\n target=\"_new\"\n class=\"product-documents__link\">\n <svg\n version=\"1.1\"\n id=\"Layer_1\"\n xmlns=\"http://www.w3.org/2000/svg\"\n xmlns:xlink=\"http://www.w3.org/1999/xlink\"\n x=\"0px\"\n y=\"0px\"\n viewBox=\"0 0 228 240\"\n style=\"enable-background: new 0 0 228 240\"\n xml:space=\"preserve\"\n class=\"product-documents__icon\"\n alt=\"16-0002 Datasheet\">\n <g>\n <path\n class=\"product-documents__svg-pdf\"\n d=\"M191.92,68.77l-47.69-47.69c-1.33-1.33-3.12-2.08-5.01-2.08H45.09C41.17,19,38,22.17,38,26.09v184.36\n c0,3.92,3.17,7.09,7.09,7.09h141.82c3.92,0,7.09-3.17,7.09-7.09V73.8C194,71.92,193.25,70.1,191.92,68.77z 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d=\"M122.57,125.68h32.84v8.49h-22.22v11.18h20.84v8.49h-20.84v20.49h-10.63V125.68z\" />\n </g>\n </svg>\n <span class=\"product-documents__info\">CUT&RUN Kit Manual</span>\n </a>\n </div>\n <div class=\"product-documents\">\n <a\n href=\"/content/documents/manuals/cut-and-run-kit-quick-card_2.0.pdf\"\n target=\"_new\"\n class=\"product-documents__link\">\n <svg\n version=\"1.1\"\n id=\"Layer_1\"\n xmlns=\"http://www.w3.org/2000/svg\"\n xmlns:xlink=\"http://www.w3.org/1999/xlink\"\n x=\"0px\"\n y=\"0px\"\n viewBox=\"0 0 228 240\"\n style=\"enable-background: new 0 0 228 240\"\n xml:space=\"preserve\"\n class=\"product-documents__icon\"\n alt=\"16-0002 Datasheet\">\n <g>\n <path\n class=\"product-documents__svg-pdf\"\n d=\"M191.92,68.77l-47.69-47.69c-1.33-1.33-3.12-2.08-5.01-2.08H45.09C41.17,19,38,22.17,38,26.09v184.36\n c0,3.92,3.17,7.09,7.09,7.09h141.82c3.92,0,7.09-3.17,7.09-7.09V73.8C194,71.92,193.25,70.1,191.92,68.77z M177.65,77.06h-41.7\n v-41.7L177.65,77.06z 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d=\"M122.57,125.68h32.84v8.49h-22.22v11.18h20.84v8.49h-20.84v20.49h-10.63V125.68z\" />\n </g>\n </svg>\n <span class=\"product-documents__info\"\n >CUT&RUN Kit Quick Card</span\n >\n </a>\n </div>\n <div class=\"product-documents\">\n <a\n href=\"/content/documents/14-1048-H3K4MetStat-Shell-Script_2.sh\"\n target=\"_blank\"\n class=\"product-documents__link\">\n <svg\n version=\"1.1\"\n id=\"Layer_1\"\n xmlns=\"http://www.w3.org/2000/svg\"\n xmlns:xlink=\"http://www.w3.org/1999/xlink\"\n x=\"0px\"\n y=\"0px\"\n viewBox=\"0 0 228 240\"\n style=\"enable-background: new 0 0 228 240\"\n xml:space=\"preserve\"\n class=\"product-documents__icon\">\n <g>\n <path\n class=\"product-documents__svg-txt\"\n d=\"M199.12,66.39l-51.16-51.16c-1.43-1.43-3.35-2.23-5.37-2.23H41.61c-4.2,0-7.61,3.41-7.61,7.61v197.79\n c0,4.2,3.41,7.61,7.61,7.61h152.14c4.2,0,7.61-3.41,7.61-7.61V71.79C201.36,69.77,200.55,67.82,199.12,66.39L199.12,66.39z\n M183.81,75.28h-44.74V30.54L183.81,75.28z M184.24,208.88H51.12V30.12h71.79v51.35c0,5.51,4.47,9.98,9.98,9.98h51.35V208.88z\n M115.78,144.7H72.04c-1.05,0-1.9,0.86-1.9,1.9v11.41c0,1.05,0.86,1.9,1.9,1.9h43.74c1.05,0,1.9-0.86,1.9-1.9V146.6\n C117.68,145.55,116.82,144.7,115.78,144.7z M70.13,114.27v11.41c0,1.05,0.86,1.9,1.9,1.9h91.29c1.05,0,1.9-0.86,1.9-1.9v-11.41\n c0-1.05-0.86-1.9-1.9-1.9H72.04C70.99,112.37,70.13,113.22,70.13,114.27z\" />\n </g>\n </svg>\n <span class=\"product-documents__info\">\n H3K4 MetStat Spike-in Controls Shell Script</span\n >\n </a>\n </div>\n <div class=\"product-documents\">\n <a\n href=\"/content/documents/H3K4-MetStat-Spike-in-Controls-Analysis_2.xlsx\"\n target=\"_blank\"\n class=\"product-documents__link\">\n <svg\n version=\"1.1\"\n id=\"Layer_1\"\n xmlns=\"http://www.w3.org/2000/svg\"\n xmlns:xlink=\"http://www.w3.org/1999/xlink\"\n x=\"0px\"\n y=\"0px\"\n viewBox=\"0 0 228 240\"\n style=\"enable-background: new 0 0 228 240\"\n xml:space=\"preserve\"\n class=\"product-documents__icon\">\n <g>\n <path\n class=\"product-documents__svg-excel\"\n d=\"M193.17,69.88l-46.83-46.83c-1.31-1.31-3.07-2.05-4.92-2.05H48.96C45.11,21,42,24.11,42,27.96v181.07\n c0,3.85,3.11,6.96,6.96,6.96h139.29c3.85,0,6.96-3.11,6.96-6.96V74.82C195.21,72.97,194.47,71.19,193.17,69.88L193.17,69.88z\n M179.15,78.02h-40.96V37.06L179.15,78.02z M179.54,200.33H57.67V36.67h65.73v47.01c0,5.05,4.09,9.14,9.14,9.14h47.01V200.33z\n M119.06,133.32l-13.45-22.29c-0.48-0.78-2.24-1.24-2.24-1.24h-8.33c-0.5,0-0.98,0.13-1.39,0.41c-1.21,0.76-1.58,2.36-0.8,3.6\n l17.85,28.28l-18.08,28.8c-0.76,1.22-0.39,2.83,0.84,3.6c0.41,0.26,0.89,0.39,1.37,0.39h7.48c0,0,1.74-0.26,2.22-1.02l13.65-22.09\n l13.56,22.07c0.48,0.78,2.22,1.26,2.22,1.26h8.18c0.5,0,0.98-0.15,1.42-0.42c1.22-0.79,1.57-2.4,0.79-3.63l-18.35-28.48\n l18.63-28.94c0.78-1.23,0.42-2.85-0.81-3.63c-0.42-0.27-0.9-0.41-1.4-0.41h-7.79c0,0-1.76,0.7-2.24,1.48L119.06,133.32z\" />\n </g>\n </svg>\n <span class=\"product-documents__info\"\n >H3K4 MetStat Spike-in Controls Analysis</span\n >\n </a>\n </div>\n </div>\n </div>\n </div>\n </div>\n</div>\n\n<style>\n /* @media screen and (min-width: 768px) {\n .products-featured .container,\n .products-featured .product-tabs,\n .products-related .container,\n .products-related .product-tabs {\n padding-left: 30px !important;\n padding-right: 50px;\n max-width: 1170px !important;\n }\n }\n @media screen and (max-width: 767px) {\n .custom-title-description {\n font-size: 19px !important;\n }\n .section-title {\n font-size: 19px !important;\n padding-left: 1rem;\n }\n } */\n\n .form-field-title .required-text {\n display: none !important;\n }\n .form-label {\n padding-left: 2rem;\n }\n .form-label-text {\n margin: 0;\n margin-left: 0 !important;\n }\n\n /* Tablet */\n #specific-table {\n width: 65%;\n }\n #specific-table-second {\n width: 75%;\n }\n #specific-row {\n width: 72% !important;\n }\n\n table {\n width: 100%;\n }\n td {\n width: 55% !important;\n }\n th {\n text-decoration: underline;\n }\n td,\n th {\n text-align: 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Description
The CUTANA™ ChIC/CUT&RUN Kit enables streamlined chromatin profiling of histone post-translational modifications (PTMs) and chromatin associated proteins while providing increased throughput and reproducibility with multi-channel pipetting.
Save 10% when you buy with our Library Prep Kit for a streamlined CUT&RUN workflow! Learn more >>
Positive (H3K4me3) and negative (IgG) control antibodies are included to pair with SNAP-CUTANA™ spike-in controls for assay optimization and continuous monitoring (Figure 2). E. coli DNA is included for data normalization. The kit is compatible with a variety of inputs including cells or nuclei derived from native, cryopreserved, or cross-linked samples. While it is recommended to start with 500,000 cells, comparable data can be generated using as few as 5,000 cells. The inclusion of controls and compatibility with diverse target types, sample inputs, and low cell numbers make this kit the go-to solution for chromatin mapping experiments. To place bulk orders, contact info@epicypher.com.
Validation Data
Figure 1: CUT&RUN DNA Fragment Size Distribution Analysis
CUT&RUN was performed using the CUTANA ChIC/CUT&RUN Kit
starting with 500,000 K-562 cells. CUT&RUN DNA, isolated
from IgG Negative Control (EpiCypher
13-0042k) and H3K4me3 Positive Control (EpiCypher
13-0041k) antibodies, was used to prepare paired-end Illumina
sequencing libraries. Library DNA was analyzed by Agilent
Tapestation®. This analysis confirmed that
mononucleosomes were predominantly enriched in CUT&RUN (~300
bp peak represents 150 bp nucleosomes + sequencing
adapters).
Figure 2: SNAP-CUTANA™ K-MetStat Spike-in Controls
DNA-barcoded designer nucleosomes (dNucs) representing 16
different K-methyl PTM states: mono-, di-, and
tri-methylation at H3K4, H3K9, H3K27, H3K36, and H4K20, as
well as unmodified control, were spiked into CUT&RUN samples
prior to the addition of the control antibodies provided
with the kit (IgG, H3K4me3). Instances of each spike-in
barcode were counted and normalized from raw fastq files
using the shell script and analysis excel sheet available on
the spike-in product page (EpiCypher
19-1002). Barcodes for IgG (top; normalized to the sum of total
reads) and H3K4me3 (bottom; normalized to on-target)
antibodies provided with this kit are shown. The spike-ins
confirmed optimal experimental conditions (H3K4me3 antibody
specifically recovered the target dNuc, while IgG showed no
preferential enrichment).
Figure 3: CUT&RUN genome-wide heatmaps
CUT&RUN data was generated using the CUTANA ChIC/CUT&RUN Kit
with 500,000 K-562 cells. Three replicates (“Rep”) of IgG
(left) and H3K4me3 (right) antibodies are shown in a
heatmap. CUT&RUN signal (from an average of 5.9 million
paired-end reads) aligned to the transcription start site
(TSS, +/- 2kb) are presented for 18,793 genes. High and low
signal are ranked by intensity (top to bottom) and reflected
by red and blue colors, respectively. Gene rows in each
heatmap are aligned and sorted from high to low signal
relative to H3K4me3 Rep 3 (far right), demonstrating the
reproducibility of the kit workflow.
Figure 4: Representative gene browser tracks
CUT&RUN data was generated as described in Figure 3. A
representative 150 kb window at the TRMT2A gene is shown for
three replicates (“Rep”) of IgG and H3K4me3 antibody
controls (included in the kit). Representative tracks are
also shown for H3K27me3 (EpiCypher 13-0030) and the
transcription factor CTCF (EMD Millipore 07-729) antibodies.
The CUT&RUN kit produced the expected genomic distribution
for each target. Images were generated using the Integrative
Genomics Viewer (IGV, Broad Institute).
Kit Contents
Item | Cat. No. |
---|---|
CUTANA™ pAG-MNase for ChIC/CUT&RUN Workflows | 15-1016 |
Histone H3K4me3 Antibody, SNAP-Certified™ for CUT&RUN and ChIP | 13-0041k |
CUTANA™ Rabbit IgG CUT&RUN Negative Control Antibody | 13-0042k |
SNAP-CUTANA™ K-MetStat Panel | 19-1002k |
CUTANA™ Concanavalin A Conjugated Paramagnetic Beads | 21-1401 |
CUTANA™ E. coli Spike-in DNA | 18-1401 |
CUTANA™ CUT&RUN 8-strip 0.2 mL Tubes | 10-0009 |
Bead Activation Buffer | 21-1001 |
Pre-Wash Buffer | 21-1002 |
Stop Buffer | 21-1003 |
5% Digitonin | 21-1004 |
1 M Spermidine | 21-1005 |
0.5 M EDTA | 21-1006 |
100 mM Calcium Chloride | 21-1007 |
DNA Cleanup Columns | 10-0010 |
DNA Collection Tubes | 10-0011 |
DNA Binding Buffer | 21-1008 |
DNA Wash Buffer | 21-1009 |
DNA Elution Buffer | 21-1010 |
Recommended Accessory Products
Item | Cat No. |
---|---|
CUTANA™ CUT&RUN Library Prep Kit | 14-1001/14-1002 |
SNAP-CUTANA™ K-MetStat Panel | 19-1002 |
CUT&RUN Antibodies | See the list |
Magnetic Separation Rack, 0.2 mL Tubes | 10-0008 |
Magnetic Separation Rack, 1.5 mL Tubes | 10-0012 |