Menin CUTANA™ CUT&RUN Antibody
19 in stock
Powered by BiozThis antibody meets EpiCypher’s “CUTANA Compatible” criteria for performance in Cleavage Under Targets and Release Using Nuclease (CUT&RUN) and/or Cleavage Under Targets and Tagmentation (CUT&Tag) approaches to genomic mapping. Every lot of a CUTANA Compatible antibody is tested in the indicated approach using EpiCypher optimized protocols and determined to yield peaks that show a genomic distribution pattern consistent with reported function(s) of the target protein. Menin antibody produces CUT&RUN peaks above background primarily in promoter and intronic regions (Figure 1) that overlap with H3K4me3 (Figure 2), consistent with its known role as a component of MLL/SET1 histone methyltransferase complex, where it specifically methylates H3K4. As a part of the MLL/SET1 complex, Menin mediates its tumor suppressor activity by regulating histone methylation of HOX and CDK inhibitor genes [1].
Trademarks and copyrights
Background References:
[1] Balogh et al. Trends Endocrinol Metab (2006). PMID: 16997566
CUT&RUN was performed on 500k K562 cells with 0.5 µg of either Menin or IgG negative control (EpiCypher 13-0042) antibodies using the CUTANA™ ChIC/CUT&RUN Kit v2.0 (EpiCypher 14-1048). Library preparation was performed with 5 ng of DNA (or the total amount recovered if less than 5 ng) using the CUTANA™ CUT&RUN Library Prep Kit (EpiCypher 14-1001/14-1002). Both kit protocols were adapted for high throughput Tecan liquid handling. Libraries were run on an Illumina NextSeq2000 with paired-end sequencing (2×50 bp). Sample sequencing depth was 5.0 million reads (IgG), 8.7 million reads (Menin), and 7.0 million reads (H3K4me3). Data were aligned to the hg19 genome using Bowtie2. Data were filtered to remove duplicates, multi-aligned reads, and ENCODE DAC Exclusion List regions.
Figure 1: Menin peaks in CUT&RUN
Figure 2: Menin CUT&RUN representative browser tracks
Figure 3: Immunohistochemistry data
Figure 4: Immunoprecipitation data
Figure 5: Western blot data
- Type: Polyclonal
- Host: Rabbit
- Applications: CUT&RUN, IHC, IP, WB
- Reactivity: Human, Mouse
- Format: Antigen affinity-purified
- Target Size: 68 kDa
Antigen affinity-purified antibody in Tris-citrate/phosphate buffer pH 7-8, 0.09% sodium azide
- CUT&RUN: 0.5 µg per reaction
- Immunohistochemistry (IHC): 1:500 – 1:2,000. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections
- Immunoprecipitation (IP): 2 – 10 µg/mg lysate
- Western Blot (WB): 1:10,000 – 1:25,000
- UniProt ID: O00255
- Gene Name: menin1, MEN1
- Protein Name: Menin
- Alternate Names: MEA1, multiple endocrine adenomatosis 1, SCG2, suppressor candidate gene 2
- Target Size: 68 kDa
