SKU: 13-2014
Size/Quantity: 100 µL

CTCF CUTANA™ CUT&RUN Antibody

$525.00

10 in stock

This antibody meets EpiCypher’s “CUTANA Compatible” criteria for performance in Cleavage Under Targets and Release Using Nuclease (CUT&RUN) and/or Cleavage Under Targets and Tagmentation (CUT&Tag) approaches to genomic mapping. Every lot of a CUTANA Compatible antibody is tested in the indicated approach using EpiCypher’s optimized protocols and determined to yield peaks that show a genomic distribution pattern consistent with reported function(s) of the target protein. CTCF regulates transcription by binding DNA and preventing interaction between promoters and nearby enhancers or silencers.

Clone BLR041F is provided for commercial sale under license from Bethyl Laboratories with additional lot-specific validation in CUT&RUN performed by EpiCypher.

CUT&RUN was performed on 500k K562 cells with either CTCF (0.125 µg) or IgG negative control (0.5 µg, EpiCypher 13-0042) antibodies using the CUTANA™ ChIC/CUT&RUN Kit v3.0 (EpiCypher 14-1048). Library preparation was performed with 5 ng of DNA (or the total amount recovered if less than 5 ng) using the CUTANA™ CUT&RUN Library Prep Kit (EpiCypher 14-1001/14-1002). Both kit protocols were adapted for high throughput Tecan liquid handling. Libraries were run on an Illumina NextSeq2000 with paired-end sequencing (2×50 bp). Sample sequencing depth was 7.6 million reads (IgG) and 10.4 million reads (CTCF). Data were aligned to the hg19 genome using Bowtie2. Data were filtered to remove duplicates, multi-aligned reads, and ENCODE DAC Exclusion List regions.

  • Type:  Monoclonal
  • Host:  Rabbit
  • Applications:  CUT&RUN, IHC, ICC, IP, WB
  • Reactivity:  Human, Mouse
  • Format:  Purified
  • Target Size: 83 kDa
Immunogen
Between amino acids 650 and 700
 
Storage
Stable for 1 year at 4°C from date of receipt
 
Formulation
Antigen affinity-purified recombinant monoclonal antibody in borate buffered saline pH 8.2, 0.1% BSA, 0.09% sodium azide
  • CUT&RUN: 0.125 µg per reaction
  • Immunohistochemistry (IHC): 1:100 – 1:1,000. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections
  • Immunoprecipitation (IP): 20 µL/mg lysate
  • Western Blot (WB): 1:1,000
  • UniProt ID: P49711
  • Gene Name: CTCF
  • Protein Name: Transcriptional repressor CTCF
  • Target Size: 83 kDa
  • Alternate Names: 11-zinc finger protein, CCCTC-binding factor, CTCFL paralog

Technical Datasheet

Clone BLR041F is provided for commercial sale under license from Bethyl Laboratories with additional lot-specific validation in CUT&RUN performed by EpiCypher.

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