CTCF CUTANA™ CUT&RUN Antibody
10 in stock
Powered by BiozThis antibody meets EpiCypher’s “CUTANA Compatible” criteria for performance in Cleavage Under Targets and Release Using Nuclease (CUT&RUN) and/or Cleavage Under Targets and Tagmentation (CUT&Tag) approaches to genomic mapping. Every lot of a CUTANA Compatible antibody is tested in the indicated approach using EpiCypher’s optimized protocols and determined to yield peaks that show a genomic distribution pattern consistent with reported function(s) of the target protein. CTCF regulates transcription by binding DNA and preventing interaction between promoters and nearby enhancers or silencers.
Trademarks and copyrights
CUT&RUN was performed on 500k K562 cells with either CTCF (0.125 µg) or IgG negative control (0.5 µg, EpiCypher 13-0042) antibodies using the CUTANA™ ChIC/CUT&RUN Kit v3.0 (EpiCypher 14-1048). Library preparation was performed with 5 ng of DNA (or the total amount recovered if less than 5 ng) using the CUTANA™ CUT&RUN Library Prep Kit (EpiCypher 14-1001/14-1002). Both kit protocols were adapted for high throughput Tecan liquid handling. Libraries were run on an Illumina NextSeq2000 with paired-end sequencing (2×50 bp). Sample sequencing depth was 7.6 million reads (IgG) and 10.4 million reads (CTCF). Data were aligned to the hg19 genome using Bowtie2. Data were filtered to remove duplicates, multi-aligned reads, and ENCODE DAC Exclusion List regions.
Figure 1: CTCF peaks in CUT&RUN
Figure 2: CTCF CUT&RUN representative browser tracks
Figure 3: Immunohistochemistry data
FFPE sections of human colon carcinoma (left) and mouse spleen (right) using CTCF antibody at a dilution of 1:1,000.
Figure 4: Immunocytochemistry data
Figure 5: Immunoprecipitation data
Figure 6: Western blot data
- Type: Monoclonal
- Host: Rabbit
- Applications: CUT&RUN, IHC, ICC, IP, WB
- Reactivity: Human, Mouse
- Format: Purified
- Target Size: 83 kDa
- CUT&RUN: 0.125 µg per reaction
- Immunohistochemistry (IHC): 1:100 – 1:1,000. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections
- Immunoprecipitation (IP): 20 µL/mg lysate
- Western Blot (WB): 1:1,000
- UniProt ID: P49711
- Gene Name: CTCF
- Protein Name: Transcriptional repressor CTCF
- Target Size: 83 kDa
- Alternate Names: 11-zinc finger protein, CCCTC-binding factor, CTCFL paralog
Clone BLR041F is provided for commercial sale under license from Bethyl Laboratories with additional lot-specific validation in CUT&RUN performed by EpiCypher.
