dCypher™ assay services combine our comprehensive library of recombinant nucleosomes and/or modified histone peptides with the high sensitivity of AlphaScreen® to discover novel chromatin interactions. This transformative technology identifies chromatin interactions that are missed by standard peptide arrays, while also requiring less starting material. As part of our high-throughput dCypher™ services, chromatin interacting proteins are screened against hundreds of histone PTMs in a single experiment, revealing the true binding specificity of chromatin readers, enzymes, antibodies, and more.
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What are the advantages of dCypher™ assay services?
EpiCypher has considerable experience in developing and optimizing chromatin binding assays, including those for chromatin readers, writer / eraser enzymes, antibodies, and more. dCypher assay services leverage this expertise, combined with our comprehensive library of modified nucleosome and histone peptide substrates, to deliver highly sensitive and customized assays. There are multiple advantages to using dCypher assay services:
- Detect chromatin interactions missed by histone peptide arrays
- Screen against physiologically relevant targets, with access to >80 modified nucleosome substrates
- Assess the binding of histone binding multiple domains in one experiment
- Reduced protein input requirements (average is nM)
- Accommodates full length proteins
- Prior knowledge of histone PTM interactions not necessary
- Have option of profiling against our library of ~300 modified histone peptides, including single and combinatorial histone PTMs
What are the service applications?
applications for dCyhper assay services include:
- Drug target identification and validation
- Reveal novel biological mechanisms
- Determine how modifications impact chromatin enzyme activity
- Examine chromatin reader binding specificity
Publications and Technical Notes
dCypher is starting to make a significant impact in epigenetics research. Learn about our most recent collaboration, in which dCypher was instrumental in revealing mechanism of DNMT3A recruitment at intergenic regions
- Weinberg DN, et al. The histone mark H3K36me2 recruits DNMT3A and shapes the intergenic DNA methylation landscape. Nature, 2019.
EpiCypher has also published a series of Technical Notes to help scientists use dCypher assays in their chromatin research.
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