Anti-Rabbit Secondary Antibody for CUTANA™ CUT&Tag Workflows
{"url":"https://www.epicypher.com/products/antibodies/cutana-cut-tag-antibodies/anti-rabbit-secondary-antibody-for-cutana-cut-tag-workflows","add_this":[{"service":"facebook","annotation":""},{"service":"email","annotation":""},{"service":"print","annotation":""},{"service":"twitter","annotation":""},{"service":"linkedin","annotation":""}],"gtin":null,"id":"770","bulk_discount_rates":[],"can_purchase":true,"meta_description":"Validated Anti-Rabbit Secondary Antibody for CUTANA™ CUT&Tag Assays","category":["Antibodies/CUTANA™ CUT&Tag Antibodies","Epigenetics Kits and Reagents/CUTANA™ CUT&Tag Assays"],"AddThisServiceButtonMeta":"","main_image":{"data":"https://cdn11.bigcommerce.com/s-y9o92/images/stencil/{:size}/products/770/756/2020_CUTTag_icon_RGB_Andy_L__75299.1592353722.1280.1280__51372.1594752212.1280.1280__02658.1595531327.jpg?c=2","alt":"Anti-Rabbit Secondary Antibody for CUTANA™ CUT&Tag Workflows"},"add_to_wishlist_url":"/wishlist.php?action=add&product_id=770","shipping":{"calculated":true},"num_reviews":0,"weight":"0.01 LBS","custom_fields":[{"id":"1173","name":"Internal Comment","value":"New lot located in bottom of Venom"}],"sku":"13-0047","description":"<div class=\"product-general-info\">\n <ul class=\"product-general-info__list-left\">\n <li class=\"product-general-info__list-item\">\n <strong>Type: </strong>Mixed Monoclonal*\n </li>\n <li class=\"product-general-info__list-item\">\n <strong>Host: </strong>Goat\n </li>\n <li class=\"product-general-info__list-item\">\n <strong>Applications: </strong>CUT&Tag\n </li>\n </ul>\n <ul class=\"product-general-info__list-right\">\n <li class=\"product-general-info__list-item\">\n <strong>Reactivity: </strong>Anti-Rabbit\n </li>\n <li class=\"product-general-info__list-item\">\n <strong>Format: </strong>Affinity Purified IgG\n </li>\n <li class=\"product-general-info__list-item\">\n <strong>Target Size: </strong>N/A\n </li>\n </ul>\n <ul class=\"product-general-info__list-right\">\n <li class=\"product-general-info__list-item\">\n <a href=\"#bioz\">\n <div\n id=\"w-s-3835-13-0047\"\n style=\"\n width: max-content;\n height: 58px;\n position: relative;\n overflow-y: hidden;\n \"></div>\n <div id=\"bioz-w-pb-13-0047-div\">\n <a\n id=\"bioz-w-pb-13-0047\"\n style=\"font-size: 12px; color: transparent\"\n href=\"https://www.bioz.com/\"\n target=\"_blank\">\n <img\n src=\"https://cdn.bioz.com/assets/favicon.png\"\n style=\"\n width: 11px;\n height: 11px;\n vertical-align: baseline;\n padding-bottom: 0px;\n margin-left: 0px;\n margin-bottom: 0px;\n float: none;\n display: none;\n \" />\n </a></div\n ></a>\n </li>\n </ul>\n</div>\n\n<div class=\"service_accordion product-droppdown\">\n <div class=\"container\">\n <div id=\"prodAccordion\">\n <div id=\"ProductDescription\" class=\"Block Panel current\">\n <h3 class=\"sub-title1\">Description</h3>\n <div\n class=\"ProductDescriptionContainer product-droppdown__section-description-specific\">\n <p>\n Anti-Rabbit Secondary Antibody for CUTANA™ CUT&Tag is affinity\n purified and specific for rabbit immunoglobulins. Minimal cross\n reactivity with mouse, rat, human, bovine, guinea pig, and donkey\n IgG is observed. Use of a secondary antibody in CUT&Tag aids in\n signal amplification by increasing bound IgG per target epitope [<a\n target=\"_blank\"\n href=\"https://pubmed.ncbi.nlm.nih.gov/31036827/\"\n >Kaya-Okur, 2019</a\n >]. For best results, use with CUTANA pAG-Tn5 (EpiCypher\n <a\n href=\"/products/epigenetics-kits-and-reagents/cutana-pag-tn5-for-cut-and-tag\"\n >15-1017 / 15-1117</a\n >).\n </p>\n\n <p>*Mixed Monoclonal: a pool of multiple recombinant monoclonal antibodies.</p>\n </div>\n </div>\n </div>\n\n <div id=\"prodAccordion\">\n <div id=\"ProductDescription\" class=\"Block Panel current\">\n <h3 class=\"sub-title1\">Validation Data</h3>\n <div\n class=\"ProductDescriptionContainer product-droppdown__section-description-specific\">\n <p>\n CUT&Tag was performed on 100k K562 nuclei with 0.5 µg of either IgG\n (EpiCypher\n <a\n href=\"/products/nucleosomes/snap-cutana-spike-in-controls/cutana-rabbit-igg-cut-run-negative-control-antibody\"\n target=\"_blank\"\n >13-0042</a\n >), H3K4me3 (EpiCypher\n <a\n href=\"/products/antibodies/snap-chip-certified-antibodies/histone-h3k4me3-antibody-snap-chip-certified-cutana-cut-run-compatible\"\n target=\"_blank\"\n >13-0041</a\n >), or H3K27me3 (ThermoFisher\n <a\n href=\"https://www.thermofisher.com/antibody/product/H3K27me3-Antibody-clone-G-299-10-Monoclonal/MA5-11198\"\n target=\"_blank\"\n >MA5-11198</a\n >) antibodies, followed by 0.5 µg of anti-rabbit secondary antibody\n for CUTANA™ CUT&Tag. The EpiCypher Direct-to-PCR CUT&Tag\n <a\n href=\"https://www.epicypher.com/resources/protocols/\"\n target=\"_blank\"\n >protocol</a\n >\n was used. Libraries were run on an Illumina NextSeq2000 with\n paired-end sequencing (2x50 bp). Sample sequencing depth was 5.0\n million reads (IgG), 6.2 million reads (H3K4me3), and 7.2 million\n reads (H3K27me3). Data were aligned to the hg19 genome using\n Bowtie2. Data were filtered to remove duplicates, multi-aligned\n reads, and ENCODE DAC Exclusion List regions.\n </p>\n <section class=\"image-picker\">\n <div class=\"image-picker__left\">\n <div\n class=\"image-picker__main-content_active image-picker__main-content\">\n <div class=\"image-picker__header-content\">\n <button class=\"image-picker__left-arrow\">\n <svg\n class=\"image-picker__svg-left\"\n width=\"24\"\n height=\"24\"\n viewBox=\"0 0 24 24\">\n <path\n d=\"M16.67 0l2.83 2.829-9.339 9.175 9.339 9.167-2.83 2.829-12.17-11.996z\" />\n </svg>\n </button>\n <a\n href=\"/content/images/products/antibodies/13-0047-cut-and-tag-data-1.jpeg\"\n target=\"_blank\"\n class=\"image-picker__main-image-link\"\n ><img\n loading=\"lazy\"\n alt=\"13-0047-cut-and-tag-data\"\n src=\"/content/images/products/antibodies/13-0047-cut-and-tag-data-1.jpeg\"\n class=\"image-picker__main-image\" />\n <span class=\"image-picker__main-image-caption\"\n >(Click to enlarge)</span\n ></a\n >\n <button class=\"image-picker__right-arrow\">\n <svg\n class=\"image-picker__svg-right\"\n width=\"24\"\n height=\"24\"\n viewBox=\"0 0 24 24\">\n <path\n d=\"M7.33 24l-2.83-2.829 9.339-9.175-9.339-9.167 2.83-2.829 12.17 11.996z\" />\n </svg>\n </button>\n </div>\n <p>\n <span class=\"image-picker__span-content\"\n ><strong>Figure 1: CUT&Tag data </strong><br />\n CUT&Tag was performed as described above. Gene browser shots\n generated using the Integrative Genomics Viewer (IGV, Broad\n Institute) show a representative 474 kb window centered at\n the LAMC3 gene. The genomic distribution pattern was\n consistent with that expected for H3K4me3 and H3K27me3.\n </span>\n </p>\n </div>\n </div>\n <aside class=\"image-picker__right\">\n <div class=\"image-picker__gallery\">\n <img\n loading=\"lazy\"\n alt=\"13-0047-cut-and-tag-data\"\n src=\"/content/images/products/antibodies/13-0047-cut-and-tag-data-1.jpeg\"\n width=\"200\"\n class=\"image-picker__side-image image-picker__side-image_active\"\n role=\"button\" />\n </div>\n </aside>\n </section>\n </div>\n </div>\n </div>\n <div id=\"prodAccordion\">\n <div id=\"ProductDescription\" class=\"Block Panel\">\n <h3 class=\"sub-title1\">Technical Information</h3>\n <div\n class=\"ProductDescriptionContainer product-droppdown__section-description\">\n <div class=\"product-tech-info\">\n <div class=\"product-tech-info__line-item\">\n <div class=\"product-tech-info__line-item-left\">\n <b>Immunogen</b>\n </div>\n <div class=\"product-tech-info__line-item-right\">\n Recombinant full-length rabbit immunoglobulin protein.\n </div>\n </div>\n <div class=\"product-tech-info__line-item\">\n <div class=\"product-tech-info__line-item-left\">\n <b>Storage and Stability</b>\n </div>\n <div class=\"product-tech-info__line-item-right\">\n Store at 4°C short term. For long term storage, store at\n -20°C. Avoid freeze/thaw cycles.\n </div>\n </div>\n <div class=\"product-tech-info__line-item\">\n <div class=\"product-tech-info__line-item-left\">\n <b>Formulation</b>\n </div>\n <div class=\"product-tech-info__line-item-right\">\n Protein G affinity-purified antibody in PBS pH 7.2, with 0.09%\n sodium azide.\n </div>\n </div>\n </div>\n </div>\n </div>\n </div>\n <div id=\"prodAccordion\">\n <div id=\"ProductDescription\" class=\"Block Panel\">\n <h3 class=\"sub-title1\">Recommended Dilution</h3>\n <div\n class=\"ProductDescriptionContainer product-droppdown__section-description\">\n <p><strong>CUT&Tag:</strong> 0.5 µg per reaction</p>\n </div>\n </div>\n </div>\n <div id=\"prodAccordion\">\n <div id=\"ProductDescription\" class=\"Block Panel\">\n <h3 class=\"sub-title1\">Documents & Resources</h3>\n <div\n class=\"ProductDescriptionContainer product-droppdown__section-description\">\n <div class=\"product-documents\">\n <a\n href=\"/content/documents/tds/13-0047.pdf\"\n target=\"_blank\"\n class=\"product-documents__link\">\n <svg\n version=\"1.1\"\n id=\"Layer_1\"\n xmlns=\"http://www.w3.org/2000/svg\"\n xmlns:xlink=\"http://www.w3.org/1999/xlink\"\n x=\"0px\"\n y=\"0px\"\n viewBox=\"0 0 228 240\"\n style=\"enable-background: new 0 0 228 240\"\n xml:space=\"preserve\"\n class=\"product-documents__icon\"\n alt=\"16-0030 Datasheet\">\n <g>\n <path\n class=\"product-documents__svg-pdf\"\n d=\"M191.92,68.77l-47.69-47.69c-1.33-1.33-3.12-2.08-5.01-2.08H45.09C41.17,19,38,22.17,38,26.09v184.36\n c0,3.92,3.17,7.09,7.09,7.09h141.82c3.92,0,7.09-3.17,7.09-7.09V73.8C194,71.92,193.25,70.1,191.92,68.77z M177.65,77.06h-41.7\n v-41.7L177.65,77.06z M178.05,201.59H53.95V34.95h66.92v47.86c0,5.14,4.17,9.31,9.31,9.31h47.86V201.59z\" />\n </g>\n <rect\n x=\"20\"\n y=\"112\"\n class=\"product-documents__svg-background\"\n width=\"146\"\n height=\"76\" />\n <g>\n <path\n class=\"product-documents__svg-pdf\"\n d=\"M23.83,125.68h22.36c5.29,0,9.41,1.33,12.35,4c2.94,2.67,4.42,6.39,4.42,11.18c0,4.78-1.47,8.51-4.42,11.18\n c-2.94,2.67-7.06,4-12.35,4H34.59v18.29H23.83V125.68z M44.81,147.9c5.38,0,8.07-2.32,8.07-6.97c0-2.39-0.67-4.16-2-5.31\n c-1.33-1.15-3.36-1.73-6.07-1.73H34.59v14.01H44.81z\" />\n <path\n class=\"product-documents__svg-pdf\"\n d=\"M69.92,125.68h18.91c5.29,0,9.84,0.97,13.66,2.9c3.82,1.93,6.74,4.72,8.76,8.35\n c2.02,3.63,3.04,7.98,3.04,13.04c0,5.06-1,9.42-3,13.08c-2,3.66-4.91,6.45-8.73,8.38c-3.82,1.93-8.4,2.9-13.73,2.9H69.92V125.68z\n M88.07,165.63c10.35,0,15.52-5.22,15.52-15.66c0-10.4-5.17-15.59-15.52-15.59h-7.38v31.26H88.07z\" />\n <path\n class=\"product-documents__svg-pdf\"\n d=\"M122.57,125.68h32.84v8.49h-22.22v11.18h20.84v8.49h-20.84v20.49h-10.63V125.68z\" />\n </g>\n </svg>\n <span class=\"product-documents__info\">Technical Datasheet</span>\n </a>\n </div>\n </div>\n </div>\n </div>\n <div id=\"prodAccordion\">\n <div id=\"ProductDescription\" class=\"Block Panel current\">\n <h3 id=\"bioz\" class=\"sub-title1\">Product References</h3>\n <div\n class=\"ProductDescriptionContainer product-droppdown__section-description-specific\"\n style=\"display: block\">\n <object\n id=\"wobj-3835-13-0047-q\"\n type=\"text/html\"\n data=\"https://www.bioz.com/v_widget_6_0/3835/13-0047/?ex=1\"\n style=\"width: 100%; height: 193px\"></object>\n <div id=\"bioz-w-pb-3835-13-0047-q-div\" style=\"width: 100%\">\n <a\n id=\"bioz-w-pb-3835-13-0047-q\"\n style=\"font-size: 12px; text-decoration: none; color: #4698cb\"\n href=\"https://www.bioz.com/\"\n target=\"_blank\"\n ><img\n src=\"https://cdn.bioz.com/assets/favicon.png\"\n style=\"\n width: 11px;\n height: 11px;\n vertical-align: baseline;\n padding-bottom: 0px;\n margin-left: 0px;\n margin-bottom: 0px;\n float: none;\n \" />\n Powered by Bioz</a\n >\n <a\n style=\"\n font-size: 12px;\n text-decoration: none;\n float: right;\n color: transparent;\n \"\n href=\"https://www.bioz.com/result/13-0047/product/EpiCypher/?cn=13-0047\"\n target=\"_blank\">\n See more details on Bioz</a\n >\n </div>\n </div>\n </div>\n </div>\n </div>\n</div>\n\n<script>\n $(document).ready(function () {\n var widget_micro_obj = new v_widget_obj('s', [1]);\n widget_micro_obj.request_catalog_number_widget_data_internal(\n '13-0047',\n '13-0047'\n );\n });\n</script>\n\n<style>\n .form-field-title .required-text {\n display: none !important;\n }\n\n .form-label {\n padding-left: 2rem;\n }\n\n .form-label-text {\n margin: 0;\n margin-left: 0 !important;\n }\n\n /* //////////// */\n\n /* BIOZ */\n td table {\n margin: 0;\n padding: 6px !important;\n }\n\n span.bioz-w-parent-hover {\n font-size: 15px;\n }\n\n td .bioz-w-tooltipx {\n padding-top: 0 !important;\n }\n /* .bioz-w-parent-hover:hover {\n text-decoration: underline white 2px !important;\n -webkit-text-decoration: underline white 2px !important;\n } */\n\n /* /////////// */\n\n .form-field-title .required-text {\n display: none !important;\n }\n .form-label {\n padding-left: 2rem;\n }\n .form-label-text {\n margin: 0;\n margin-left: 0 !important;\n }\n\n .bioz-w-header td {\n padding: 0;\n }\n\n .epicypher-table table {\n margin-right: auto;\n margin-left: auto;\n width: 100%;\n border-top: 1px solid lightgray;\n }\n\n .epicypher-table th {\n color: #3b3a48;\n }\n .epicypher-table td {\n color: #3b3a48;\n font-size: 0.9rem;\n }\n .epicypher-table td a {\n text-decoration: underline;\n }\n .epicypher-table td,\n th {\n border: 0.5px solid lightgray;\n text-align: left;\n padding: 8px;\n /* white-space: nowrap; 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- Type: Mixed Monoclonal*
- Host: Goat
- Applications: CUT&Tag
- Reactivity: Anti-Rabbit
- Format: Affinity Purified IgG
- Target Size: N/A
Description
Anti-Rabbit Secondary Antibody for CUTANA™ CUT&Tag is affinity purified and specific for rabbit immunoglobulins. Minimal cross reactivity with mouse, rat, human, bovine, guinea pig, and donkey IgG is observed. Use of a secondary antibody in CUT&Tag aids in signal amplification by increasing bound IgG per target epitope [Kaya-Okur, 2019]. For best results, use with CUTANA pAG-Tn5 (EpiCypher 15-1017 / 15-1117).
*Mixed Monoclonal: a pool of multiple recombinant monoclonal antibodies.
Validation Data
CUT&Tag was performed on 100k K562 nuclei with 0.5 µg of either IgG (EpiCypher 13-0042), H3K4me3 (EpiCypher 13-0041), or H3K27me3 (ThermoFisher MA5-11198) antibodies, followed by 0.5 µg of anti-rabbit secondary antibody for CUTANA™ CUT&Tag. The EpiCypher Direct-to-PCR CUT&Tag protocol was used. Libraries were run on an Illumina NextSeq2000 with paired-end sequencing (2x50 bp). Sample sequencing depth was 5.0 million reads (IgG), 6.2 million reads (H3K4me3), and 7.2 million reads (H3K27me3). Data were aligned to the hg19 genome using Bowtie2. Data were filtered to remove duplicates, multi-aligned reads, and ENCODE DAC Exclusion List regions.
Figure 1: CUT&Tag data
CUT&Tag was performed as described above. Gene browser shots
generated using the Integrative Genomics Viewer (IGV, Broad
Institute) show a representative 474 kb window centered at
the LAMC3 gene. The genomic distribution pattern was
consistent with that expected for H3K4me3 and H3K27me3.
Technical Information
Recommended Dilution
CUT&Tag: 0.5 µg per reaction