CUTANA™ Concanavalin A Conjugated Paramagnetic Beads
{"sku":"21-1401","url":"https://www.epicypher.com/products/epigenetics-kits-and-reagents/cutana-chic-cut-run-assays/cutana-concanavalin-a-conjugated-paramagnetic-beads","add_this":[{"service":"facebook","annotation":""},{"service":"email","annotation":""},{"service":"print","annotation":""},{"service":"twitter","annotation":""},{"service":"linkedin","annotation":""}],"gtin":null,"id":"762","bulk_discount_rates":[],"can_purchase":true,"meta_description":"CUTANA™ Concanavalin A Conjugated Paramagnetic Beads for ChIC CUT&RUN and ChIP-seq","category":["Epigenetics Kits and Reagents/CUTANA™ ChIC / CUT&RUN Assays","Epigenetics Kits and Reagents/CUTANA™ CUT&Tag Assays"],"AddThisServiceButtonMeta":"","main_image":{"data":"https://cdn11.bigcommerce.com/s-y9o92/images/stencil/{:size}/products/762/745/Screen_Shot_2020-02-12_at_11.01.55_AM__44591.1592491196.png?c=2","alt":"CUTANA™ Concanavalin A Conjugated Paramagnetic Beads"},"add_to_wishlist_url":"/wishlist.php?action=add&product_id=762","shipping":{"calculated":true},"num_reviews":0,"weight":"0.01 LBS","description":"<div class=\"product-general-info\">\n <ul style=\"display: none\" class=\"product-general-info__list-left\">\n <li class=\"product-general-info__list-item\"></li>\n <li class=\"product-general-info__list-item\"></li>\n </ul>\n <ul style=\"display: none\" class=\"product-general-info__list-right\">\n <li class=\"product-general-info__list-item\"></li>\n <li class=\"product-general-info__list-item\"></li>\n </ul>\n <ul style=\"padding: 0\" class=\"product-general-info__list-right\">\n <li class=\"product-general-info__list-item\">\n <a href=\"#bioz\">\n <div\n id=\"w-s-3835-21-1401\"\n style=\"\n width: max-content;\n height: 58px;\n position: relative;\n overflow-y: hidden;\n \"></div>\n <div id=\"bioz-w-pb-21-1401-div\">\n <a\n id=\"bioz-w-pb-21-1401\"\n style=\"font-size: 12px; color: transparent\"\n href=\"https://www.bioz.com/\"\n target=\"_blank\">\n <img\n src=\"https://cdn.bioz.com/assets/favicon.png\"\n style=\"\n width: 11px;\n height: 11px;\n vertical-align: baseline;\n padding-bottom: 0px;\n margin-left: 0px;\n margin-bottom: 0px;\n float: none;\n display: none;\n \" />\n </a></div\n ></a>\n </li>\n </ul>\n</div>\n<div class=\"service_accordion product-droppdown\">\n <div class=\"container\">\n <div id=\"prodAccordion\">\n <div id=\"ProductDescription\" class=\"Block Panel current\">\n <h3 class=\"sub-title1\">Description</h3>\n <div\n class=\"ProductDescriptionContainer product-droppdown__section-description\"\n style=\"display: block\">\n <p>\n Available in 50 and 250 Reaction pack sizes. This product contains\n Concanavalin A (ConA) conjugated to paramagnetic microspheres. ConA\n is a lectin (carbohydrate-binding protein) that binds specifically\n to mannosyl- and glucosyl-containing extracellular glycoproteins.\n The ConA magnetic beads are therefore useful to immobilize cells or\n nuclei presenting these glycans in their extracellular matrices.\n </p>\n </div>\n </div>\n </div>\n <div id=\"prodAccordion\">\n <div id=\"ProductDescription\" class=\"Block Panel current\">\n <h3 class=\"sub-title1\">Validation Data</h3>\n <div\n class=\"ProductDescriptionContainer product-droppdown__section-description\"\n style=\"display: block\">\n <section class=\"image-picker\">\n <div class=\"image-picker__left\">\n <div\n class=\"image-picker__main-content_active image-picker__main-content\">\n <div class=\"image-picker__header-content\">\n <button class=\"image-picker__left-arrow\">\n <svg\n class=\"image-picker__svg-left\"\n width=\"24\"\n height=\"24\"\n viewBox=\"0 0 24 24\">\n <path\n d=\"M16.67 0l2.83 2.829-9.339 9.175 9.339 9.167-2.83 2.829-12.17-11.996z\" />\n </svg>\n </button>\n <a\n href=\"/content/images/products/genomics/21_1401_TSS.jpg\"\n target=\"_blank\"\n class=\"image-picker__main-image-link\"\n ><img\n loading=\"lazy\"\n alt=\"21-1401 TSS\"\n src=\"/content/images/products/genomics/21_1401_TSS.jpg\"\n class=\"image-picker__main-image\" />\n <span class=\"image-picker__main-image-caption\"\n >(Click to enlarge)</span\n ></a\n >\n <button class=\"image-picker__right-arrow\">\n <svg\n class=\"image-picker__svg-right\"\n width=\"24\"\n height=\"24\"\n viewBox=\"0 0 24 24\">\n <path\n d=\"M7.33 24l-2.83-2.829 9.339-9.175-9.339-9.167 2.83-2.829 12.17 11.996z\" />\n </svg>\n </button>\n </div>\n <p>\n <span class=\"image-picker__span-content\"\n ><strong>Figure 1: </strong><br />CUT&RUN data was generated\n using EpiCypher CUTANA™ Concanavalin A-Conjugated\n Paramagnetic Beads (10 µL) with 500,000 K562 cells and\n H3K4me3 antibody (EpiCypher Catalog No.\n <a\n href=\"/products/antibodies/snap-chip-certified-antibodies/histone-h3k4me3-antibody-snap-chip-certified-cutana-cut-run-compatible\"\n >13-0041</a\n >, 1:100 dilution). Shown is a heatmap for H3K4me3 CUT&RUN\n signal (from 3.9 million paired-end reads) aligned to the\n transcription start site (TSS, +/- 2kb) of 18,793 genes.\n High and low signal are ranked by intensity (top to bottom)\n and reflected by red and blue colors, respectively.\n </span>\n </p>\n </div>\n <div class=\"image-picker__main-content\">\n <div class=\"image-picker__header-content\">\n <button class=\"image-picker__left-arrow\">\n <svg\n class=\"image-picker__svg-left\"\n width=\"24\"\n height=\"24\"\n viewBox=\"0 0 24 24\">\n <path\n d=\"M16.67 0l2.83 2.829-9.339 9.175 9.339 9.167-2.83 2.829-12.17-11.996z\" />\n </svg>\n </button>\n <a\n href=\"/content/images/products/genomics/21_1401_track.jpg\"\n target=\"_blank\"\n class=\"image-picker__main-image-link\"\n ><img\n loading=\"lazy\"\n alt=\"21-1401 CUT&RUN\"\n src=\"/content/images/products/genomics/21_1401_track.jpg\"\n class=\"image-picker__main-image\" />\n <span class=\"image-picker__main-image-caption\"\n >(Click to enlarge)</span\n ></a\n >\n <button class=\"image-picker__right-arrow\">\n <svg\n class=\"image-picker__svg-right\"\n width=\"24\"\n height=\"24\"\n viewBox=\"0 0 24 24\">\n <path\n d=\"M7.33 24l-2.83-2.829 9.339-9.175-9.339-9.167 2.83-2.829 12.17 11.996z\" />\n </svg>\n </button>\n </div>\n <p>\n <span class=\"image-picker__span-content\"\n ><strong>Figure 2: </strong><br />\n <!-- CUT&RUN data was generated as described above. Three\n representative loci are shown in the Integrative Genomics\n Viewer (IGV, Broad Institute). Window size is denoted at the\n top of each panel. Top tracks at each locus were generated\n using H3K4me3 antibody (EpiCypher Catalog No.\n <a\n href=\"/products/antibodies/snap-chip-certified-antibodies/histone-h3k4me3-antibody-snap-chip-certified-cutana-cut-run-compatible\"\n >13-0041</a\n >, 0.5 µg), while the bottom tracks were generated using IgG\n Negative control antibody (EpiCypher Catalog No.\n <a\n href=\"/products/epigenetics-reagents-and-assays/cutana-chic-cut-run-assays/cutana-rabbit-igg-cut-run-negative-control-antibody\"\n >13-0042</a\n >, 0.5 µg). -->\n H3K4me3 CUT&RUN data was generated as described above. Four\n representative loci are shown in the Integrative Genomics\n Viewer (IGV, Broad Institute). Window size is denoted at the\n top of each panel.\n </span>\n </p>\n </div>\n </div>\n <aside class=\"image-picker__right\">\n <div class=\"image-picker__gallery\">\n <img\n loading=\"lazy\"\n alt=\"21_1401_TSS\"\n src=\"/content/images/products/genomics/21_1401_TSS.jpg\"\n width=\"200\"\n class=\"image-picker__side-image image-picker__side-image_active\"\n role=\"button\" />\n <img\n loading=\"lazy\"\n alt=\"21_1401_track\"\n src=\"/content/images/products/genomics/21_1401_track.jpg\"\n class=\"image-picker__side-image\"\n role=\"button\" />\n </div>\n </aside>\n </section>\n </div>\n </div>\n </div>\n <div id=\"prodAccordion\">\n <div id=\"ProductDescription\" class=\"Block Panel\">\n <h3 class=\"sub-title1\">Technical Information</h3>\n <div\n class=\"ProductDescriptionContainer product-droppdown__section-description\">\n <div class=\"product-tech-info\">\n <div class=\"product-tech-info__line-item\">\n <div class=\"product-tech-info__line-item-left\">\n <b>Formulation</b>\n </div>\n <div class=\"product-tech-info__line-item-right\">\n Concanavalin A conjugated to 1 µm paramagnetic microspheres in\n 10mM PBS with 0.1% sodium azide.\n </div>\n </div>\n <div class=\"product-tech-info__line-item\">\n <div class=\"product-tech-info__line-item-left\">\n <b>Storage and Stability</b>\n </div>\n <div class=\"product-tech-info__line-item-right\">\n Stable for six months at 4°C from date of receipt.\n <strong>DO NOT FREEZE!</strong>\n </div>\n </div>\n </div>\n </div>\n </div>\n </div>\n <div id=\"prodAccordion\">\n <div id=\"ProductDescription\" class=\"Block Panel\">\n <h3 class=\"sub-title1\">Application Notes</h3>\n <div\n class=\"ProductDescriptionContainer product-droppdown__section-description\">\n <p>\n <em\n >*Note: It is recommended to use 10 µL slurry per sample\n (500,000 cells or less) for ChIC/CUT&RUN. Compatible with the\n EpiCypher Magnetic Separation Rack (Catalog Nos.\n <a\n href=\"/products/epigenetics-reagents-and-assays/cutana-chic-cut-run-assays/magnetic-separation-rack-0-2-ml-tubes\"\n title=\"EpiCypher Magenetic Separation Rack\"\n target=\"new\"\n >10-0008</a\n >\n &\n </em>\n <a\n href=\"/products/epigenetics-reagents-and-assays/magnetic-separation-rack-1-5-ml-tubes\"\n >10-0012</a\n >).\n </p>\n </div>\n </div>\n </div>\n <!-- <div id=\"prodAccordion\">\n <div id=\"ProductDescription\" class=\"Block Panel\">\n <h3 class=\"sub-title1\">References</h3>\n <div\n class=\"ProductDescriptionContainer product-droppdown__section-description\">\n <strong>Background References:</strong>\n <br />\n Lai <em>et al.</em> <em>bioRxiv</em> (2020).\n <a\n href=\"https://www.biorxiv.org/content/10.1101/2020.06.08.140046v1\"\n target=\"_blank\"\n title=\"Screening of PCRP transcription factor antibodies in biochemical assays\"\n >(2020.06.08.140046)</a\n ><br />\n <br />\n <strong>Product References:</strong>\n\n <iframe\n class=\"airtable-embed\"\n src=\"https://airtable.com/embed/shrlHaNTE2WfBXZik?backgroundColor=purple&viewControls=on\"\n frameborder=\"0\"\n onmousewheel=\"\"\n width=\"100%\"\n height=\"300\"\n style=\"background: transparent; border: 1px solid #ccc\"></iframe>\n </div>\n </div>\n </div> -->\n <div id=\"prodAccordion\">\n <div id=\"ProductDescription\" class=\"Block Panel\">\n <h3 class=\"sub-title1\">Documents & Resources</h3>\n <div\n class=\"ProductDescriptionContainer product-droppdown__section-description\">\n <div class=\"product-documents\">\n <a\n href=\"/content/documents/tds/21-1401.pdf\"\n target=\"_blank\"\n class=\"product-documents__link\">\n <svg\n version=\"1.1\"\n id=\"Layer_1\"\n xmlns=\"http://www.w3.org/2000/svg\"\n xmlns:xlink=\"http://www.w3.org/1999/xlink\"\n x=\"0px\"\n y=\"0px\"\n viewBox=\"0 0 228 240\"\n style=\"enable-background: new 0 0 228 240\"\n xml:space=\"preserve\"\n class=\"product-documents__icon\"\n alt=\"21-1401 Datasheet\">\n <g>\n <path\n class=\"product-documents__svg-pdf\"\n d=\"M191.92,68.77l-47.69-47.69c-1.33-1.33-3.12-2.08-5.01-2.08H45.09C41.17,19,38,22.17,38,26.09v184.36\n c0,3.92,3.17,7.09,7.09,7.09h141.82c3.92,0,7.09-3.17,7.09-7.09V73.8C194,71.92,193.25,70.1,191.92,68.77z M177.65,77.06h-41.7\n v-41.7L177.65,77.06z M178.05,201.59H53.95V34.95h66.92v47.86c0,5.14,4.17,9.31,9.31,9.31h47.86V201.59z\" />\n </g>\n <rect\n x=\"20\"\n y=\"112\"\n class=\"product-documents__svg-background\"\n width=\"146\"\n height=\"76\" />\n <g>\n <path\n class=\"product-documents__svg-pdf\"\n d=\"M23.83,125.68h22.36c5.29,0,9.41,1.33,12.35,4c2.94,2.67,4.42,6.39,4.42,11.18c0,4.78-1.47,8.51-4.42,11.18\n c-2.94,2.67-7.06,4-12.35,4H34.59v18.29H23.83V125.68z M44.81,147.9c5.38,0,8.07-2.32,8.07-6.97c0-2.39-0.67-4.16-2-5.31\n c-1.33-1.15-3.36-1.73-6.07-1.73H34.59v14.01H44.81z\" />\n <path\n class=\"product-documents__svg-pdf\"\n d=\"M69.92,125.68h18.91c5.29,0,9.84,0.97,13.66,2.9c3.82,1.93,6.74,4.72,8.76,8.35\n c2.02,3.63,3.04,7.98,3.04,13.04c0,5.06-1,9.42-3,13.08c-2,3.66-4.91,6.45-8.73,8.38c-3.82,1.93-8.4,2.9-13.73,2.9H69.92V125.68z\n M88.07,165.63c10.35,0,15.52-5.22,15.52-15.66c0-10.4-5.17-15.59-15.52-15.59h-7.38v31.26H88.07z\" />\n <path\n class=\"product-documents__svg-pdf\"\n d=\"M122.57,125.68h32.84v8.49h-22.22v11.18h20.84v8.49h-20.84v20.49h-10.63V125.68z\" />\n </g>\n </svg>\n <span class=\"product-documents__info\">Technical Datasheet</span>\n </a>\n </div>\n <div class=\"product-documents\">\n <a\n href=\"/resources/protocols/cutana-cut-and-run-protocol/\"\n target=\"blank\"\n class=\"product-documents__link\">\n <svg\n version=\"1.1\"\n id=\"Layer_1\"\n xmlns=\"http://www.w3.org/2000/svg\"\n xmlns:xlink=\"http://www.w3.org/1999/xlink\"\n x=\"0px\"\n y=\"0px\"\n viewBox=\"0 0 228 240\"\n style=\"enable-background: new 0 0 228 240\"\n xml:space=\"preserve\"\n class=\"product-documents__icon\"\n alt=\"CUTANAâ„¢ CUT&RUN Protocol\">\n <g>\n <path\n class=\"product-documents__svg-link\"\n d=\"M130.3,157.06c-0.77-0.76-2-0.76-2.77,0l-28.45,28.45c-13.17,13.17-35.41,14.57-49.95,0\n c-14.57-14.57-13.17-36.78,0-49.95l28.45-28.45c0.76-0.76,0.76-2.01,0-2.77l-9.75-9.75c-0.77-0.76-2-0.76-2.77,0l-28.45,28.45\n c-20.71,20.71-20.71,54.23,0,74.92s54.23,20.71,74.92,0L140,169.52c0.76-0.76,0.76-2.01,0-2.77L130.3,157.06z M193.62,41\n c-20.71-20.71-54.23-20.71-74.92,0L90.22,69.45c-0.76,0.77-0.76,2,0,2.77l9.72,9.72c0.76,0.76,2.01,0.76,2.77,0l28.45-28.45\n c13.17-13.17,35.41-14.57,49.95,0c14.57,14.57,13.17,36.78,0,49.95l-28.45,28.45c-0.76,0.77-0.76,2,0,2.77l9.75,9.75\n c0.76,0.76,2.01,0.76,2.77,0l28.45-28.45C214.31,95.23,214.31,61.71,193.62,41L193.62,41z M139.14,85.29c-0.77-0.76-2-0.76-2.77,0\n l-55.46,55.43c-0.76,0.77-0.76,2,0,2.77l9.7,9.7c0.76,0.76,2.01,0.76,2.77,0l55.43-55.43c0.76-0.76,0.76-2.01,0-2.77L139.14,85.29z\n \" />\n </g>\n </svg>\n <span class=\"product-documents__info\">CUT&RUN Protocol</span>\n </a>\n </div>\n </div>\n </div>\n </div>\n <div id=\"prodAccordion\">\n <div id=\"ProductDescription\" class=\"Block Panel current\">\n <h3 id=\"bioz\" class=\"sub-title1\">Product References</h3>\n <div\n class=\"ProductDescriptionContainer product-droppdown__section-description-specific\">\n <object\n id=\"wobj-3835-21-1401-q\"\n type=\"text/html\"\n data=\"https://www.bioz.com/v_widget_6_0/3835/21-1401/?ex=1\"\n style=\"width: 100%; height: 193px\"></object>\n <div id=\"bioz-w-pb-3835-21-1401-q-div\" style=\"width: 100%\">\n <a\n id=\"bioz-w-pb-3835-21-1401-q\"\n style=\"font-size: 12px; text-decoration: none; color: #4698cb\"\n href=\"https://www.bioz.com/\"\n target=\"_blank\"\n ><img\n src=\"https://cdn.bioz.com/assets/favicon.png\"\n style=\"\n width: 11px;\n height: 11px;\n vertical-align: baseline;\n padding-bottom: 0px;\n margin-left: 0px;\n margin-bottom: 0px;\n float: none;\n \" />\n Powered by Bioz</a\n >\n <a\n style=\"\n font-size: 12px;\n text-decoration: none;\n float: right;\n color: transparent;\n \"\n href=\"https://www.bioz.com/result/21-1401/product/EpiCypher/?cn=21-1401\"\n target=\"_blank\">\n See more details on Bioz</a\n >\n </div>\n </div>\n </div>\n </div>\n </div>\n</div>\n\n<script>\n $(document).ready(function () {\n var widget_micro_obj = new v_widget_obj('s', [1]);\n widget_micro_obj.request_catalog_number_widget_data_internal(\n '21-1401',\n '21-1401'\n );\n });\n</script>\n\n<style>\n .form-field-title .required-text {\n display: none !important;\n }\n\n .form-label {\n padding-left: 2rem;\n }\n\n .form-label-text {\n margin: 0;\n margin-left: 0 !important;\n }\n\n /* //////////// */\n\n /* BIOZ */\n td table {\n margin: 0;\n padding: 6px !important;\n }\n\n span.bioz-w-parent-hover {\n font-size: 15px;\n }\n\n td .bioz-w-tooltipx {\n padding-top: 0 !important;\n }\n /* .bioz-w-parent-hover:hover {\n text-decoration: underline !important;\n } */\n\n /* /////////// */\n\n .form-field-title .required-text {\n display: none !important;\n }\n .form-label {\n padding-left: 2rem;\n }\n .form-label-text {\n margin: 0;\n margin-left: 0 !important;\n }\n\n .bioz-w-header td {\n padding: 0;\n }\n\n .epicypher-table table {\n margin-right: auto;\n margin-left: auto;\n width: 100%;\n border-top: 1px solid lightgray;\n }\n\n .epicypher-table th {\n color: #3b3a48;\n }\n .epicypher-table td {\n color: #3b3a48;\n font-size: 0.9rem;\n }\n .epicypher-table td a {\n text-decoration: underline;\n }\n .epicypher-table td,\n th {\n border: 0.5px solid lightgray;\n text-align: left;\n padding: 8px;\n /* white-space: nowrap; 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Description
Available in 50 and 250 Reaction pack sizes. This product contains Concanavalin A (ConA) conjugated to paramagnetic microspheres. ConA is a lectin (carbohydrate-binding protein) that binds specifically to mannosyl- and glucosyl-containing extracellular glycoproteins. The ConA magnetic beads are therefore useful to immobilize cells or nuclei presenting these glycans in their extracellular matrices.
Validation Data
Figure 1:
CUT&RUN data was generated
using EpiCypher CUTANA™ Concanavalin A-Conjugated
Paramagnetic Beads (10 µL) with 500,000 K562 cells and
H3K4me3 antibody (EpiCypher Catalog No.
13-0041, 1:100 dilution). Shown is a heatmap for H3K4me3 CUT&RUN
signal (from 3.9 million paired-end reads) aligned to the
transcription start site (TSS, +/- 2kb) of 18,793 genes.
High and low signal are ranked by intensity (top to bottom)
and reflected by red and blue colors, respectively.