Magnetic Separation Rack, 0.2 mL Tubes
{"sku":"10-0008","url":"https://www.epicypher.com/products/epigenetics-reagents-and-assays/cutana-chic-cut-run-assays/magnetic-separation-rack-0-2-ml-tubes","add_this":[{"service":"facebook","annotation":""},{"service":"email","annotation":""},{"service":"print","annotation":""},{"service":"twitter","annotation":""},{"service":"linkedin","annotation":""}],"gtin":null,"id":757,"bulk_discount_rates":[],"can_purchase":true,"meta_description":" Magnetic Separation Rack for 0.2 mL Tubes and NGS","category":["Epigenetics Kits and Reagents/CUTANA™ ChIC / CUT&RUN Assays","Epigenetics Kits and Reagents/CUTANA™ CUT&Tag Assays"],"AddThisServiceButtonMeta":"","main_image":{"data":"https://cdn11.bigcommerce.com/s-y9o92/images/stencil/{:size}/products/757/973/magnetic-separation-rack-0.2-ml-tubes__20679.1645734565.jpg?c=2","alt":"Magnetic Separation Rack, 0.2 mL Tubes"},"add_to_wishlist_url":"/wishlist.php?action=add&product_id=757","shipping":{"calculated":true},"num_reviews":0,"weight":"0.00 LBS","description":"<div class=\"service_accordion product-droppdown\">\n <div class=\"container\">\n <div id=\"prodAccordion\">\n <div id=\"ProductDescription\" class=\"Block Panel current\">\n <h3 class=\"sub-title1\">Description</h3>\n <div\n class=\"ProductDescriptionContainer product-droppdown__section-description-specific\"\n >\n <p>\n Magnetic Separation Rack for use with up to sixteen 0.2 mL PCR tubes\n for separation of paramagnetic beads in a variety of cell-based or\n molecular biology applications, including Cleavage Under Targets and\n Release Using Nuclease (<a href=\"/technologies/cutana/cut-and-run\"\n >CUT&RUN</a\n >) and Cleavage Under Targets and Tagmentation (<a\n href=\"https://www.epicypher.com/technologies/cutana/cut-and-tag\"\n >CUT&Tag</a\n >).\n </p>\n </div>\n </div>\n </div>\n <div id=\"prodAccordion\">\n <div id=\"ProductDescription\" class=\"Block Panel current\">\n <h3 class=\"sub-title1\">Product Images</h3>\n <div\n class=\"ProductDescriptionContainer product-droppdown__section-description-specific\"\n >\n <section class=\"image-picker\">\n <div class=\"image-picker__left\">\n <div\n class=\"image-picker__main-content_active image-picker__main-content\"\n >\n <div class=\"image-picker__header-content\">\n <button class=\"image-picker__left-arrow\">\n <svg\n class=\"image-picker__svg-left\"\n width=\"24\"\n height=\"24\"\n viewBox=\"0 0 24 24\"\n >\n <path\n d=\"M16.67 0l2.83 2.829-9.339 9.175 9.339 9.167-2.83 2.829-12.17-11.996z\"\n />\n </svg>\n </button>\n <a\n href=\"/content/images/products/genomics/10_0008_front.jpg\"\n target=\"_blank\"\n class=\"image-picker__main-image-link\"\n ><img loading=\"lazy\"\n alt=\"10_0008_front\"\n src=\"/content/images/products/genomics/10_0008_front.jpg\"\n class=\"image-picker__main-image\"\n />\n <span class=\"image-picker__main-image-caption\"\n >(Click to enlarge)</span\n ></a\n >\n <button class=\"image-picker__right-arrow\">\n <svg\n class=\"image-picker__svg-right\"\n width=\"24\"\n height=\"24\"\n viewBox=\"0 0 24 24\"\n >\n <path\n d=\"M7.33 24l-2.83-2.829 9.339-9.175-9.339-9.167 2.83-2.829 12.17 11.996z\"\n />\n </svg>\n </button>\n </div>\n <p>\n <span class=\"image-picker__span-content\"\n ><strong\n >Figure 1: Magnetic Separation Rack, front view</strong\n ><br />\n Note that the 5 - 50 μL is up, and that the magnets are\n toward the bottom of the rack, so that the beads will be\n sorted near the bottom of the tubes.\n </span>\n </p>\n </div>\n <div class=\"image-picker__main-content\">\n <div class=\"image-picker__header-content\">\n <button class=\"image-picker__left-arrow\">\n <svg\n class=\"image-picker__svg-left\"\n width=\"24\"\n height=\"24\"\n viewBox=\"0 0 24 24\"\n >\n <path\n d=\"M16.67 0l2.83 2.829-9.339 9.175 9.339 9.167-2.83 2.829-12.17-11.996z\"\n />\n </svg>\n </button>\n <a\n href=\"/content/images/products/genomics/10_0008_quarterview.jpg\"\n target=\"_blank\"\n class=\"image-picker__main-image-link\"\n ><img loading=\"lazy\"\n alt=\"10_0008_quarterview\"\n src=\"/content/images/products/genomics/10_0008_quarterview.jpg\"\n class=\"image-picker__main-image\"\n />\n <span class=\"image-picker__main-image-caption\"\n >(Click to enlarge)</span\n ></a\n >\n <button class=\"image-picker__right-arrow\">\n <svg\n class=\"image-picker__svg-right\"\n width=\"24\"\n height=\"24\"\n viewBox=\"0 0 24 24\"\n >\n <path\n d=\"M7.33 24l-2.83-2.829 9.339-9.175-9.339-9.167 2.83-2.829 12.17 11.996z\"\n />\n </svg>\n </button>\n </div>\n <p>\n <span class=\"image-picker__span-content\"\n ><strong\n >Figure 2: Magnetic Separation Rack, oblique view</strong\n ><br />\n Rack shown with PCR strip tubes and paramagnetic beads. Note\n that the 50 - 200 μL configuration is being used, and that\n the magnets are sorting the beads to the side of the tubes.\n </span>\n </p>\n </div>\n </div>\n <aside class=\"image-picker__right\">\n <div class=\"image-picker__gallery\">\n <img loading=\"lazy\"\n alt=\"10_0008_front\"\n src=\"/content/images/products/genomics/10_0008_front.jpg\"\n width=\"200\"\n class=\"image-picker__side-image image-picker__side-image_active\"\n role=\"button\"\n />\n <img loading=\"lazy\"\n alt=\"10_0008_quarterview\"\n src=\"/content/images/products/genomics/10_0008_quarterview.jpg\"\n class=\"image-picker__side-image\"\n role=\"button\"\n />\n </div>\n </aside>\n </section>\n </div>\n </div>\n </div>\n <div id=\"prodAccordion\">\n <div id=\"ProductDescription\" class=\"Block Panel\">\n <h3 class=\"sub-title1\">Features</h3>\n <div\n class=\"ProductDescriptionContainer product-droppdown__section-description\"\n >\n <ul>\n <li>\n Solid aluminum construction with hard coat anodizing for\n durability\n </li>\n <li>\n Strong permanent Neodymium magnets for fast and effective bead\n separation\n </li>\n <li>\n Compatible with standard 0.2 mL PCR tubes, either as individual\n tubes or strip tubes\n </li>\n <li>\n Double-sided for simultaneous processing of up to 16 samples\n </li>\n <li>\n Can be used on either small volumes (5 - 50 μL,\n <strong>Figure 1</strong>) or larger volumes (50 - 200 μL,\n <strong>Figure 2</strong>)\n </li>\n </ul>\n </div>\n </div>\n </div>\n <div id=\"prodAccordion\">\n <div id=\"ProductDescription\" class=\"Block Panel\">\n <h3 class=\"sub-title1\">Application Notes</h3>\n <div\n class=\"ProductDescriptionContainer product-droppdown__section-description\"\n >\n <p>\n Suitable for cell-based sorting and molecular biology applications\n using paramagnetic beads, including CUTANA™\n <a href=\"/technologies/cutana/cut-and-run\">CUT&RUN</a> and\n <a href=\"/technologies/cutana/cut-and-tag\">CUT&Tag</a> technologies.\n Also needed for the\n <a\n href=\"/products/epigenetics-reagents-and-assays/cutana-cut-and-run-library-prep-kit\"\n >CUT&RUN Library Prep Kit</a\n >.\n </p>\n </div>\n </div>\n </div>\n <div id=\"prodAccordion\">\n <div id=\"ProductDescription\" class=\"Block Panel\">\n <h3 class=\"sub-title1\">Documents & Resources</h3>\n <div\n class=\"ProductDescriptionContainer product-droppdown__section-description\"\n >\n <div class=\"product-documents\">\n <a\n href=\"/content/documents/tds/10-0008.pdf\"\n target=\"_blank\"\n class=\"product-documents__link\"\n >\n <svg\n version=\"1.1\"\n id=\"Layer_1\"\n xmlns=\"http://www.w3.org/2000/svg\"\n xmlns:xlink=\"http://www.w3.org/1999/xlink\"\n x=\"0px\"\n y=\"0px\"\n viewBox=\"0 0 228 240\"\n style=\"enable-background: new 0 0 228 240\"\n xml:space=\"preserve\"\n class=\"product-documents__icon\"\n alt=\"16-0030 Datasheet\"\n >\n <g>\n <path\n class=\"product-documents__svg-pdf\"\n 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c2.02,3.63,3.04,7.98,3.04,13.04c0,5.06-1,9.42-3,13.08c-2,3.66-4.91,6.45-8.73,8.38c-3.82,1.93-8.4,2.9-13.73,2.9H69.92V125.68z\n M88.07,165.63c10.35,0,15.52-5.22,15.52-15.66c0-10.4-5.17-15.59-15.52-15.59h-7.38v31.26H88.07z\"\n />\n <path\n class=\"product-documents__svg-pdf\"\n d=\"M122.57,125.68h32.84v8.49h-22.22v11.18h20.84v8.49h-20.84v20.49h-10.63V125.68z\"\n />\n </g>\n </svg>\n <span class=\"product-documents__info\">Technical Datasheet</span>\n </a>\n </div>\n </div>\n </div>\n </div>\n </div>\n</div>\n","tags":[],"warranty":"","price":{"without_tax":{"formatted":"$495.00","value":495,"currency":"USD"},"tax_label":"Sales Tax"},"detail_messages":"","availability":"","page_title":" Magnetic Separation Rack for 0.2 mL Tubes ","cart_url":"https://www.epicypher.com/cart.php","max_purchase_quantity":0,"mpn":null,"upc":null,"options":[],"related_products":[{"id":986,"sku":null,"name":"CUTANA™ CUT&Tag Kit","url":"https://www.epicypher.com/products/epigenetics-kits-and-reagents/cutana-cut-and-tag-kit","availability":"","rating":null,"brand":{"name":null},"category":["Antibodies/CUTANA™ CUT&Tag Antibodies","Epigenetics Kits and Reagents/CUTANA™ CUT&Tag Assays"],"summary":"\n \n \n \n \n ","image":{"data":"https://cdn11.bigcommerce.com/s-y9o92/images/stencil/{:size}/products/986/1213/CTK20__46720.1713641110.png?c=2","alt":"CUTANA™ CUT&Tag Kit"},"images":[{"data":"https://cdn11.bigcommerce.com/s-y9o92/images/stencil/{:size}/products/986/1213/CTK20__46720.1713641110.png?c=2","alt":"CUTANA™ CUT&Tag Kit"}],"date_added":"8th Mar 2023","pre_order":false,"show_cart_action":true,"has_options":true,"stock_level":null,"low_stock_level":null,"qty_in_cart":0,"custom_fields":[{"id":1219,"name":"Pack Size","value":"48 Reactions"}],"num_reviews":null,"weight":{"formatted":"0.01 LBS","value":0.01},"demo":false,"price":{"without_tax":{"currency":"USD","formatted":"$2,695.00","value":2695},"tax_label":"Sales Tax"},"add_to_wishlist_url":"/wishlist.php?action=add&product_id=986"},{"id":694,"sku":null,"name":"CUTANA™ pAG-MNase for ChIC/CUT&RUN Workflows","url":"https://www.epicypher.com/products/epigenetics-reagents-and-assays/cutana-pag-mnase-for-chic-cut-and-run-workflows","availability":"","rating":null,"brand":{"name":null},"category":["Epigenetics Kits and Reagents","Epigenetics Kits and Reagents/CUTANA™ ChIC / CUT&RUN Assays"],"summary":"\n \n \n Type: Nuclease\n \n \n Mol Wgt: 43.7 kDa\n \n \n \n ","image":{"data":"https://cdn11.bigcommerce.com/s-y9o92/images/stencil/{:size}/products/694/689/Screen_Shot_2020-02-12_at_11.01.55_AM__17144.1581530752.png?c=2","alt":"CUTANA™ pAG-MNase for ChIC/CUT&RUN Workflows"},"images":[{"data":"https://cdn11.bigcommerce.com/s-y9o92/images/stencil/{:size}/products/694/689/Screen_Shot_2020-02-12_at_11.01.55_AM__17144.1581530752.png?c=2","alt":"CUTANA™ pAG-MNase for ChIC/CUT&RUN Workflows"}],"date_added":"12th Aug 2019","pre_order":false,"show_cart_action":true,"has_options":true,"stock_level":null,"low_stock_level":null,"qty_in_cart":0,"custom_fields":[{"id":1174,"name":"Internal Comment","value":"Excess in bottom of Venom"},{"id":1175,"name":"Internal Comment","value":"Bulk in Psylocke"}],"num_reviews":null,"weight":{"formatted":"0.01 LBS","value":0.01},"demo":false,"price":{"without_tax":{"currency":"USD","formatted":"$335.00","value":335},"price_range":{"min":{"without_tax":{"currency":"USD","formatted":"$335.00","value":335},"tax_label":"Sales Tax"},"max":{"without_tax":{"currency":"USD","formatted":"$1,295.00","value":1295},"tax_label":"Sales Tax"}},"tax_label":"Sales Tax"},"add_to_wishlist_url":"/wishlist.php?action=add&product_id=694"},{"id":764,"sku":null,"name":"CUTANA™ pAG-Tn5 for CUT&Tag","url":"https://www.epicypher.com/products/epigenetics-kits-and-reagents/cutana-pag-tn5-for-cut-and-tag","availability":"","rating":null,"brand":{"name":null},"category":["Epigenetics Kits and Reagents","Epigenetics Kits and Reagents/CUTANA™ CUT&Tag Assays"],"summary":"\n \n \n Type: Transposase\n \n \n Mol Wgt: 191 kDa\n \n \n \n ","image":{"data":"https://cdn11.bigcommerce.com/s-y9o92/images/stencil/{:size}/products/764/750/2020_CUTTag_icon_RGB_Andy_L__93238.1592491196.png?c=2","alt":"CUTANA™ pAG-Tn5 for CUT&Tag"},"images":[{"data":"https://cdn11.bigcommerce.com/s-y9o92/images/stencil/{:size}/products/764/750/2020_CUTTag_icon_RGB_Andy_L__93238.1592491196.png?c=2","alt":"CUTANA™ pAG-Tn5 for CUT&Tag"}],"date_added":"17th Jun 2020","pre_order":false,"show_cart_action":true,"has_options":true,"stock_level":null,"low_stock_level":null,"qty_in_cart":0,"custom_fields":[{"id":1005,"name":"Internal Comment","value":"extra boxes in bottom shelf of Venom"}],"num_reviews":null,"weight":{"formatted":"0.01 LBS","value":0.01},"demo":false,"price":{"without_tax":{"currency":"USD","formatted":"$795.00","value":795},"price_range":{"min":{"without_tax":{"currency":"USD","formatted":"$795.00","value":795},"tax_label":"Sales Tax"},"max":{"without_tax":{"currency":"USD","formatted":"$2,995.00","value":2995},"tax_label":"Sales Tax"}},"tax_label":"Sales Tax"},"add_to_wishlist_url":"/wishlist.php?action=add&product_id=764"}],"shipping_messages":[],"rating":0,"meta_keywords":" Magnetic Separation Rack for 0.2 mL Tubes , CUTANA, CUT&RUN, CUT&TAG, ChIP-seq, NGS","show_quantity_input":1,"title":"Magnetic Separation Rack, 0.2 mL Tubes","gift_wrapping_available":false,"min_purchase_quantity":0,"customizations":[],"images":[{"data":"https://cdn11.bigcommerce.com/s-y9o92/images/stencil/{:size}/products/757/973/magnetic-separation-rack-0.2-ml-tubes__20679.1645734565.jpg?c=2","alt":"Magnetic Separation Rack, 0.2 mL Tubes"}]}
Description
CUTANA™ Bead Activation Buffer is used to prepare Concanavalin A (ConA) conjugated paramagnetic beads for CUT&RUN and CUT&Tag. Activated ConA beads are used in both CUT&RUN and CUT&Tag workflows to immobilize cells or nuclei.
Validation Data
(Click to enlarge)
ConA beads were washed with CUTANA™ Bead Activation Buffer and loaded with K562 cells as described in the CUTANA™ CUT&RUN and CUT&Tag protocols (www.epicypher.com/protocols). Trypan Blue staining shows cells before (panels A and B) and after (C and D) bead binding. After incubation with activated beads, the supernatant contains few unbound cells (C), demonstrating efficient binding. Permeabilized cells bound to activated beads are observed in the cell-bead slurry (D).
Recommended Companion Products
ITEM | CAT. NO. |
---|---|
CUTANA™ ChIC/CUT&RUN Kit | 14-1048/14-1048-24 |
CUTANA™ CUT&RUN Library Prep Kit | 14-1001/14-1002 |
CUTANA™ CUT&Tag Kit | 14-1102/14-1103 |
CUTANA™ pAG-MNase for ChIC/CUT&RUN | 15-1016/15-1116 |
CUTANA™ pAG-Tn5 for CUT&Tag | 15-1017/15-1117 |
CUTANA™ Concanavalin A Conjugated Paramagnetic Beads | 21-1401/21-1411 |
CUTANA™ Nuclei Extraction Buffer | 21-1026 |
CUTANA™ E. coli Spike-in DNA | 18-1401 |
Magnetic Separation Rack, 0.2 mL/1.5 mL Tubes | 10-0008/10-0012 |
CUTANA™ CUT&RUN 8-strip 0.2 mL Tubes | 10-0009 |
Technical Information
Documents & Resources
Description
CUTANA™ Stop Buffer is used in CUT&RUN to terminate pAG-MNase activity and prevent over-digestion of released DNA fragments. The Stop Buffer is added following pAG-MNase incubation, and halts enzyme activity by chelating free calcium ions.
Validation Data
(Click to enlarge)
Recommended Companion Products
ITEM | CAT. NO. |
---|---|
CUTANA™ ChIC/CUT&RUN Kit | 14-1048/14-1048-24 |
CUTANA™ CUT&RUN Library Prep Kit | 14-1001/14-1002 |
CUTANA™ pAG-MNase for ChIC/CUT&RUN | 15-1016/15-1116 |
CUTANA™ Concanavalin A Conjugated Paramagnetic Beads | 21-1401/21-1411 |
CUTANA™ Nuclei Extraction Buffer | 21-1026 |
CUTANA™ E. coli Spike-in DNA | 18-1401 |
Magnetic Separation Rack, 0.2 mL/1.5 mL Tubes | 10-0008/10-0012 |
CUTANA™ CUT&RUN 8-strip 0.2 mL Tubes | 10-0009 |
Technical Information
Documents & Resources
Description
Digitonin is a nonionic detergent used in CUTANA™ CUT&RUN and CUT&Tag genomic mapping assays to permeabilize cells, facilitating antibody and enzyme access to chromatin. Digitonin also helps prevent clumping of Concanavalin A (ConA) magnetic beads and film formation on tubes, which minimizes sample loss. For this reason, Digitonin is recommended in assay buffers for both cell and nuclei samples. Refer to the appropriate kit manual or do-it-yourself (DIY) protocol for guidance on which assay buffers require Digitonin.
Validation Data
(Click to enlarge)
Recommended Companion Products
ITEM | CAT. NO. |
---|---|
CUTANA™ ChIC/CUT&RUN Kit | 14-1048/14-1048-24 |
CUTANA™ CUT&RUN Library Prep Kit | 14-1001/14-1002 |
CUTANA™ CUT&Tag Kit | 14-1102/14-1103 |
CUTANA™ pAG-MNase for ChIC/CUT&RUN | 15-1016/15-1116 |
CUTANA™ pAG-Tn5 for CUT&Tag | 15-1017/15-1117 |
CUTANA™ Concanavalin A Conjugated Paramagnetic Beads | 21-1401/21-1411 |
CUTANA™ Nuclei Extraction Buffer | 21-1026 |
CUTANA™ E. coli Spike-in DNA | 18-1401 |
Magnetic Separation Rack, 0.2 mL/1.5 mL Tubes | 10-0008/10-0012 |
CUTANA™ CUT&RUN 8-strip 0.2 mL Tubes | 10-0009 |
Technical Information
See www.epicypher.com/protocols for detailed protocols. Visit support.epicypher.com for guidance on optimizing digitonin concentration.
Documents & Resources
Description
Product Images
Figure 1: Magnetic Separation Rack, front view
Note that the 5 - 50 μL is up, and that the magnets are
toward the bottom of the rack, so that the beads will be
sorted near the bottom of the tubes.
Features
- Solid aluminum construction with hard coat anodizing for durability
- Strong permanent Neodymium magnets for fast and effective bead separation
- Compatible with standard 0.2 mL PCR tubes, either as individual tubes or strip tubes
- Double-sided for simultaneous processing of up to 16 samples
- Can be used on either small volumes (5 - 50 μL, Figure 1) or larger volumes (50 - 200 μL, Figure 2)
Application Notes
Suitable for cell-based sorting and molecular biology applications using paramagnetic beads, including CUTANA™ CUT&RUN and CUT&Tag technologies. Also needed for the CUT&RUN Library Prep Kit.