CUTANA™

CUTANA™ Protease Inhibitor Tablets

$235.00
SKU: 21-1027-S
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Description

This EDTA-free cocktail inhibits various serine and cysteine proteases, resulting in stabilization of target proteins isolated from animal tissues, plants, and yeast for in situ assays like ChIC/CUT&RUN (EpiCypher 14-1048) or CUT&Tag (EpiCypher 14-1102/14-1103). Absence of EDTA and other chelating agents is essential for the controlled activation of pAG-MNase (EpiCypher 15-1016/15-1116) and pAG-Tn5 (EpiCypher 15-1017/15-1117), but leaves metallo- and aspartic proteases inhibited. This product is also used in the preparation of CUTANA™ Nuclei Extraction Buffer (EpiCypher 21-1026).

Technical Information

Storage
Tablets stored in their original container are stable at 4ºC until the date of expiration on the label.
Formulation
Proprietary mixture of protease inhibitors; EDTA free.
Instructions for Use
Reconstitute each tablet in 420 µL (15-1027-S) or 2 mL (15-1027-L) molecular biology-grade water to prepare a 25X stock. Leftover 25X protease inhibitor can be stored for 12 weeks at -20ºC.
Reactions/Tablet (approx.)
15-1027-S 15-1027-L
CUT&RUN 5 reactions 27 reactions
CUT&Tag 6 reactions 30 reactions
Nuclei Extraction 40 reactions 204 reactions
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