SNAP-ChIP® Dual Labeled Hydrolysis Probe: Universal SNAP-ChIP® Dual Labeled Hydrolysis Probe is
compatible with all EpiCypher SNAP-ChIP products. This
24 bp ssDNA qPCR probe contains a 5’ 6-FAM, a single
isomer dervative of fluorescein, and an interal ZEN/3’
Iowa Black QC quencher pair, which reduces background
signal. When reconstituted in 50 μL H2O to yield a final
concentration of 5 μM (20X), the volume is sufficient to
perform 100 x 10 μL qPCR reactions (see SNAP-ChIP
SNAP-ChIP® Dual Labeled Hydrolysis Probe Formulation:100 qPCR reactions of SNAP-ChIP Dual Labeled Hydrolysis
Probe as lyophilyzed DNA. Prior to use, reconstitute DNA
in 50 μL Molecular Biology Grade H2O to yield 5 μM (20X)
SNAP-ChIP® Dual Labeled Hydrolysis Probe Storage and Stability: Stable for 2 years at -20°C from date of receipt. After
resuspending, aliquots should be stored at -20°C. Protect from light for long term storage.
DNA Sequence: 5’-6-FAM-TCTAGCACC-ZEN-GCTTAAACGCACGTA-IABkFQ-3’
View technical datasheet for this product.
SNAP-ChIP qPCR Data.A) SNAP-ChIP Dual Labeled Hydrolysis Probe was tested in a ChIP-qPCR experiment using input chromatin from human K-562 cells spiked-in with the SNAP-ChIP K-MetStat Panel
(EpiCypher Catalog No. 19-1001). TaqMan™ qPCR using SNAP-ChIP Dual Labeled Hydrolysis Probe paired with primer sets specific to each nucleosome in the K-MetStat Panel (x-axis) shows amplification of
each nucleosome target in the SNAP-ChIP spiked input chromatin compared to no template control. Data shown is for triplicate qPCR reactions in a single ChIP experiment. B) Representative dilution series
using a SNAP-ChIP barcoded DNA template subjected to qPCR amplification with the Taq probe and corresponding primer pair. The SNAP-ChIP primer/probe set demonstrated the expected 2-fold
amplification per cycle and amplified the target with 100.5% efficiency.