

Histone H3K9me1 Antibody, SNAP-Certified™ for CUT&RUN and ChIP
{"AddThisServiceButtonMeta":"","add_this":[{"annotation":"","service":"facebook"},{"annotation":"","service":"email"},{"annotation":"","service":"print"},{"annotation":"","service":"twitter"},{"annotation":"","service":"linkedin"}],"add_to_wishlist_url":"/wishlist.php?action=add&product_id=627","availability":"","bulk_discount_rates":[],"can_purchase":true,"cart_url":"https://www.epicypher.com/cart.php","category":["Antibodies/CUTANA™ CUT&RUN Antibodies","Antibodies/CUTANA™ CUT&RUN Antibodies/CUT&RUN Antibodies - Histone PTMs","Antibodies/SNAP-ChIP<sup>®</sup> Certified Antibodies"],"custom_fields":[{"id":"453","name":"Pack Size","value":"100 μg"}],"customizations":[],"description":"<div class=\"product-general-info\">\n <ul class=\"product-general-info__list-left\">\n <li class=\"product-general-info__list-item\">\n <strong>Type: </strong>Monoclonal\n </li>\n <li class=\"product-general-info__list-item\">\n <strong>Target Size: </strong>15 kDa\n </li>\n <li class=\"product-general-info__list-item\">\n <strong>Format: </strong>Affinity Purified IgG\n </li>\n </ul>\n <ul class=\"product-general-info__list-right\">\n <li class=\"product-general-info__list-item\">\n <strong>Host: </strong>Rabbit\n </li>\n <li class=\"product-general-info__list-item\">\n <strong>Reactivity: </strong>Human, Mouse, Wide Range\n (Predicted)\n </li>\n <li class=\"product-general-info__list-item\">\n <strong>Applications: </strong>CUT&RUN, ChIP, ICC, WB\n </li>\n </ul>\n</div>\n\n<div class=\"service_accordion product-droppdown\">\n <div class=\"container\">\n <div id=\"prodAccordion\">\n <div id=\"ProductDescription\" class=\"Block Panel current\">\n <h3 class=\"sub-title1\">Description</h3>\n <div\n class=\"ProductDescriptionContainer product-droppdown__section-description-specific\"\n >\n <p>\n This antibody meets EpiCypher’s lot-specific SNAP-Certified™\n criteria for specificity and efficient target enrichment in both\n CUT&RUN and ChIP applications. In CUT&RUN, this requires <20%\n cross-reactivity to related histone PTMs determined using the\n SNAP-CUTANA™ K-MetStat Panel of spike-in controls (EpiCypher\n <a\n href=\"https://www.epicypher.com/products/nucleosomes/snap-cutana-k-metstat-panel\"\n >19-1002</a\n >, <strong>Figure 1</strong>). High target efficiency is confirmed\n by consistent genomic enrichment at 500k and 50k starting cells\n (<strong>Figures 2-3</strong>). In ChIP, this requires <20%\n cross-reactivity to related histone PTMs and >5% of target input\n recovered as determined using the SNAP-ChIP<sup>®</sup> K-MetStat\n Panel of spike-in controls (EpiCypher\n <a\n href=\"https://www.epicypher.com/products/nucleosomes/snap-chip-k-metstat-panel\"\n >19-1001</a\n >, <strong>Figure 6</strong>) [1].\n </p>\n </div>\n </div>\n </div>\n <div id=\"prodAccordion\">\n <div id=\"ProductDescription\" class=\"Block Panel current\">\n <h3 class=\"sub-title1\">Validation Data</h3>\n <div\n class=\"ProductDescriptionContainer product-droppdown__section-description-specific\"\n >\n <section class=\"image-picker\">\n <div class=\"image-picker__left\">\n <div\n class=\"image-picker__main-content_active image-picker__main-content\"\n >\n <div class=\"image-picker__header-content\">\n <button class=\"image-picker__left-arrow\">\n <svg\n class=\"image-picker__svg-left\"\n width=\"24\"\n height=\"24\"\n viewBox=\"0 0 24 24\"\n >\n <path\n d=\"M16.67 0l2.83 2.829-9.339 9.175 9.339 9.167-2.83 2.829-12.17-11.996z\"\n />\n </svg>\n </button>\n <a\n href=\"/content/images/products/antibodies/13-0029-specificity-analysis.jpeg\"\n target=\"_blank\"\n class=\"image-picker__main-image-link\"\n ><img\n alt=\"13-0029-specificity-analysis\"\n src=\"/content/images/products/antibodies/13-0029-specificity-analysis.jpeg\"\n class=\"image-picker__main-image\"\n />\n <span class=\"image-picker__main-image-caption\"\n >(Click to enlarge)</span\n ></a\n >\n <button class=\"image-picker__right-arrow\">\n <svg\n class=\"image-picker__svg-right\"\n width=\"24\"\n height=\"24\"\n viewBox=\"0 0 24 24\"\n >\n <path\n d=\"M7.33 24l-2.83-2.829 9.339-9.175-9.339-9.167 2.83-2.829 12.17 11.996z\"\n />\n </svg>\n </button>\n </div>\n <p>\n <span class=\"image-picker__span-content\"\n ><strong\n >Figure 1: SNAP specificity analysis in CUT&RUN </strong\n ><br />\n CUT&RUN was performed on 500k and 50k native K562 cells with\n the SNAP-CUTANA K-MetStat Panel (EpiCypher\n <a\n href=\"https://www.epicypher.com/products/nucleosomes/snap-cutana-k-metstat-panel\"\n >19-1002</a\n >) spiked-in prior to the addition of 0.5 µg H3K9me1\n antibody. CUT&RUN sequencing reads were aligned to the\n unique DNA barcodes corresponding to each nucleosome in the\n K-MetStat panel (x-axis). Data are expressed as a percent\n relative to on-target recovery (H3K9me1 set to 100%). Error\n bars represent mean ± SEM for the duplicate DNA barcodes in\n a single CUT&RUN experiment.\n </span>\n </p>\n </div>\n <div class=\"image-picker__main-content\">\n <div class=\"image-picker__header-content\">\n <button class=\"image-picker__left-arrow\">\n <svg\n class=\"image-picker__svg-left\"\n width=\"24\"\n height=\"24\"\n viewBox=\"0 0 24 24\"\n >\n <path\n d=\"M16.67 0l2.83 2.829-9.339 9.175 9.339 9.167-2.83 2.829-12.17-11.996z\"\n />\n </svg>\n </button>\n <a\n href=\"/content/images/products/antibodies/13-0029-efficiency-analysis.jpeg\"\n target=\"_blank\"\n class=\"image-picker__main-image-link\"\n ><img\n alt=\"13-0029-efficiency-analysis\"\n src=\"/content/images/products/antibodies/13-0029-efficiency-analysis.jpeg\"\n class=\"image-picker__main-image\"\n />\n <span class=\"image-picker__main-image-caption\"\n >(Click to enlarge)</span\n ></a\n >\n <button class=\"image-picker__right-arrow\">\n <svg\n class=\"image-picker__svg-right\"\n width=\"24\"\n height=\"24\"\n viewBox=\"0 0 24 24\"\n >\n <path\n d=\"M7.33 24l-2.83-2.829 9.339-9.175-9.339-9.167 2.83-2.829 12.17 11.996z\"\n />\n </svg>\n </button>\n </div>\n <p>\n <span class=\"image-picker__span-content\"\n ><strong\n >Figure 2: Antibody efficiency analysis in CUT&RUN using\n cell input correlation </strong\n ><br />\n Genome-wide correlation analysis was performed to compare\n H3K9me1 antibody enrichment in CUT&RUN using 500k and 50k\n cell inputs. The log of the number of reads per 75 bp binned\n region across the genome is plotted for both samples.\n CUT&RUN data generated using this H3K9me1 antibody are\n highly correlated between the two cell inputs (Pearson\n correlation r = 0.973), indicating high consistency of\n H3K9me1 antibody target recovery.\n </span>\n </p>\n </div>\n <div class=\"image-picker__main-content\">\n <div class=\"image-picker__header-content\">\n <button class=\"image-picker__left-arrow\">\n <svg\n class=\"image-picker__svg-left\"\n width=\"24\"\n height=\"24\"\n viewBox=\"0 0 24 24\"\n >\n <path\n d=\"M16.67 0l2.83 2.829-9.339 9.175 9.339 9.167-2.83 2.829-12.17-11.996z\"\n />\n </svg>\n </button>\n <a\n href=\"/content/images/products/antibodies/13-0029-representative-tracks.jpeg\"\n target=\"_blank\"\n class=\"image-picker__main-image-link\"\n >\n <img\n alt=\"13-0029-representative-tracks\"\n src=\"/content/images/products/antibodies/13-0029-representative-tracks.jpeg\"\n class=\"image-picker__main-image\"\n />\n <span class=\"image-picker__main-image-caption\"\n >(Click to enlarge)</span\n >\n </a>\n <button class=\"image-picker__right-arrow\">\n <svg\n class=\"image-picker__svg-right\"\n width=\"24\"\n height=\"24\"\n viewBox=\"0 0 24 24\"\n >\n <path\n d=\"M7.33 24l-2.83-2.829 9.339-9.175-9.339-9.167 2.83-2.829 12.17 11.996z\"\n />\n </svg>\n </button>\n </div>\n <p>\n <span class=\"image-picker__span-content\">\n <strong\n >Figure 3: H3K9me1 CUT&RUN representative tracks at 500k\n and 50k cells </strong\n ><br />\n Gene browser shots generated using the Integrative Genomics\n Viewer (IGV, Broad Institute) show representative loci for\n H3K9me1 antibody tested in CUT&RUN using 500k and 50k cells.\n Similar results in peak structure and location were observed\n for both cell inputs.\n <em\n ><u>Methods:</u> CUT&RUN was performed using the CUTANA™\n ChIC/CUT&RUN Kit v2.0 (EpiCypher\n <a\n href=\"https://www.epicypher.com/products/epigenetics-reagents-and-assays/cutana-chic-cut-and-run-kit\"\n >14-1048</a\n >) adapted to high throughput Tecan liquid handling.\n Library preparation was performed with 5 ng DNA using the\n NEBNext<sup>®</sup> Ultra™ II DNA Library Prep Kit for\n Illumina<sup>®</sup>. Libraries were run on an Illumina\n NextSeq2000 with paired-end sequencing (2x50 bp). Sample\n sequencing depth was 4.5 million reads (IgG 50k cell\n input), 14.2 million reads (H3K9me1 50k cell input), and\n 10.1 million reads (H3K9me1 500k cell input).</em\n >\n </span>\n </p>\n </div>\n <div class=\"image-picker__main-content\">\n <div class=\"image-picker__header-content\">\n <button class=\"image-picker__left-arrow\">\n <svg\n class=\"image-picker__svg-left\"\n width=\"24\"\n height=\"24\"\n viewBox=\"0 0 24 24\"\n >\n <path\n d=\"M16.67 0l2.83 2.829-9.339 9.175 9.339 9.167-2.83 2.829-12.17-11.996z\"\n />\n </svg>\n </button>\n <a\n href=\"/content/images/products/antibodies/13-0029-immunocytochemistry.jpeg\"\n target=\"_blank\"\n class=\"image-picker__main-image-link\"\n >\n <img\n alt=\"13-0029-immunocytochemistry\"\n src=\"/content/images/products/antibodies/13-0029-immunocytochemistry.jpeg\"\n class=\"image-picker__main-image\"\n />\n <span class=\"image-picker__main-image-caption\"\n >(Click to enlarge)</span\n >\n </a>\n <button class=\"image-picker__right-arrow\">\n <svg\n class=\"image-picker__svg-right\"\n width=\"24\"\n height=\"24\"\n viewBox=\"0 0 24 24\"\n >\n <path\n d=\"M7.33 24l-2.83-2.829 9.339-9.175-9.339-9.167 2.83-2.829 12.17 11.996z\"\n />\n </svg>\n </button>\n </div>\n <p>\n <span class=\"image-picker__span-content\">\n <strong>Figure 4: Immunocytochemistry </strong><br />\n ICC of HeLa cells using 2 µg/mL of H3K9me1 antibody (red).\n Actin filaments were labeled with fluorescein phalloidin\n (green).\n </span>\n </p>\n </div>\n <div class=\"image-picker__main-content\">\n <div class=\"image-picker__header-content\">\n <button class=\"image-picker__left-arrow\">\n <svg\n class=\"image-picker__svg-left\"\n width=\"24\"\n height=\"24\"\n viewBox=\"0 0 24 24\"\n >\n <path\n d=\"M16.67 0l2.83 2.829-9.339 9.175 9.339 9.167-2.83 2.829-12.17-11.996z\"\n />\n </svg>\n </button>\n <a\n href=\"/content/images/products/antibodies/13-0029-western-blot-data.jpeg\"\n target=\"_blank\"\n class=\"image-picker__main-image-link\"\n >\n <img\n alt=\"13-0029-western-blot-data\"\n src=\"/content/images/products/antibodies/13-0029-western-blot-data.jpeg\"\n class=\"image-picker__main-image\"\n />\n <span class=\"image-picker__main-image-caption\"\n >(Click to enlarge)</span\n >\n </a>\n <button class=\"image-picker__right-arrow\">\n <svg\n class=\"image-picker__svg-right\"\n width=\"24\"\n height=\"24\"\n viewBox=\"0 0 24 24\"\n >\n <path\n d=\"M7.33 24l-2.83-2.829 9.339-9.175-9.339-9.167 2.83-2.829 12.17 11.996z\"\n />\n </svg>\n </button>\n </div>\n <p>\n <span class=\"image-picker__span-content\">\n <strong>Figure 5: Western blot data </strong><br />\n Recombinant histone H3.3 (Lane 1) and acid extracts of HeLa\n cells (Lane 2) were blotted onto PVDF and probed with 0.5\n µg/mL H3K9me1 antibody.\n </span>\n </p>\n </div>\n <div class=\"image-picker__main-content\">\n <div class=\"image-picker__header-content\">\n <button class=\"image-picker__left-arrow\">\n <svg\n class=\"image-picker__svg-left\"\n width=\"24\"\n height=\"24\"\n viewBox=\"0 0 24 24\"\n >\n <path\n d=\"M16.67 0l2.83 2.829-9.339 9.175 9.339 9.167-2.83 2.829-12.17-11.996z\"\n />\n </svg>\n </button>\n <a\n href=\"/content/images/products/antibodies/13-0029-specificity-and-enrichment-analysis.jpeg\"\n target=\"_blank\"\n class=\"image-picker__main-image-link\"\n >\n <img\n alt=\"13-0029-specificity-and-enrichment-analysis\"\n src=\"/content/images/products/antibodies/13-0029-specificity-and-enrichment-analysis.jpeg\"\n class=\"image-picker__main-image\"\n />\n <span class=\"image-picker__main-image-caption\"\n >(Click to enlarge)</span\n >\n </a>\n <button class=\"image-picker__right-arrow\">\n <svg\n class=\"image-picker__svg-right\"\n width=\"24\"\n height=\"24\"\n viewBox=\"0 0 24 24\"\n >\n <path\n d=\"M7.33 24l-2.83-2.829 9.339-9.175-9.339-9.167 2.83-2.829 12.17 11.996z\"\n />\n </svg>\n </button>\n </div>\n <p>\n <span class=\"image-picker__span-content\">\n <strong\n >Figure 6: SNAP-ChIP-qPCR specificity and enrichment\n analysis </strong\n ><br />\n Histone H3K9me1 antibody (3 µg) was tested in a native ChIP\n experiment using chromatin from K562 cells (3 µg) with the\n SNAP-ChIP K-MetStat Panel (EpiCypher\n <a\n href=\"https://www.epicypher.com/products/nucleosomes/snap-chip-k-metstat-panel\"\n >19-1001</a\n >) spiked-in prior to micrococcal nuclease digestion.\n Specificity (left y-axis) was determined by qPCR for the DNA\n barcodes corresponding to modified nucleosomes in the\n SNAP-ChIP panel (x-axis). Black bar represents antibody\n efficiency (right y-axis; log scale) and indicates\n percentage of the target immunoprecipitated relative to\n input. Error bars represent mean ± SEM in replicate ChIP\n experiments.\n </span>\n </p>\n </div>\n </div>\n <aside class=\"image-picker__right\">\n <div class=\"image-picker__gallery\">\n <img\n alt=\"13-0029-specificity-analysis\"\n src=\"/content/images/products/antibodies/13-0029-specificity-analysis.jpeg\"\n width=\"200\"\n class=\"image-picker__side-image image-picker__side-image_active\"\n role=\"button\"\n />\n <img\n alt=\"13-0029-efficiency-analysis\"\n src=\"/content/images/products/antibodies/13-0029-efficiency-analysis.jpeg\"\n class=\"image-picker__side-image\"\n role=\"button\"\n />\n <img\n alt=\"13-0029-representative-tracks\"\n src=\"/content/images/products/antibodies/13-0029-representative-tracks.jpeg\"\n class=\"image-picker__side-image\"\n role=\"button\"\n />\n <img\n alt=\"13-0029-immunocytochemistry\"\n src=\"/content/images/products/antibodies/13-0029-immunocytochemistry.jpeg\"\n class=\"image-picker__side-image\"\n role=\"button\"\n />\n <img\n alt=\"13-0029-western-blot-data\"\n src=\"/content/images/products/antibodies/13-0029-western-blot-data.jpeg\"\n class=\"image-picker__side-image\"\n role=\"button\"\n />\n <img\n alt=\"13-0029-specificity-and-enrichment-analysis\"\n src=\"/content/images/products/antibodies/13-0029-specificity-and-enrichment-analysis.jpeg\"\n class=\"image-picker__side-image\"\n role=\"button\"\n />\n </div>\n </aside>\n </section>\n </div>\n </div>\n </div>\n <div id=\"prodAccordion\">\n <div id=\"ProductDescription\" class=\"Block Panel\">\n <h3 class=\"sub-title1\">Technical Information</h3>\n <div\n class=\"ProductDescriptionContainer product-droppdown__section-description\"\n >\n <div class=\"product-tech-info\">\n <div class=\"product-tech-info__line-item\">\n <div class=\"product-tech-info__line-item-left\">\n <b>Immunogen</b>\n </div>\n <div class=\"product-tech-info__line-item-right\">\n A synthetic peptide corresponding to histone H3 monomethylated\n at lysine 9.\n </div>\n </div>\n <div class=\"product-tech-info__line-item\">\n <div class=\"product-tech-info__line-item-left\">\n <b>Formulation</b>\n </div>\n <div class=\"product-tech-info__line-item-right\">\n Protein A affinity-purified antibody in PBS, with 0.09% sodium\n azide, 1% BSA, and 50% glycerol.\n </div>\n </div>\n <div class=\"product-tech-info__line-item\">\n <div class=\"product-tech-info__line-item-left\">\n <b>Storage and Stability</b>\n </div>\n <div class=\"product-tech-info__line-item-right\">\n Stable for 1 year at -20°C from date of receipt.\n </div>\n </div>\n </div>\n </div>\n </div>\n </div>\n <div id=\"prodAccordion\">\n <div id=\"ProductDescription\" class=\"Block Panel\">\n <h3 class=\"sub-title1\">Recommended Dilution</h3>\n <div\n class=\"ProductDescriptionContainer product-droppdown__section-description\"\n >\n <p><strong>CUT&RUN:</strong> 0.5 µg per reaction</p>\n <p>\n <strong>Chromatin Immunoprecipitation (ChIP):</strong> 2 - 5 µg per\n 5 µg chromatin\n </p>\n <p>\n <strong>Immunofluorescence/Immunocytochemistry (IF/ICC):</strong>\n 0.5 - 2 µg/mL\n </p>\n <p><strong>Western Blot (WB):</strong> 0.2 - 1 µg/mL</p>\n </div>\n </div>\n </div>\n <div id=\"prodAccordion\">\n <div id=\"ProductDescription\" class=\"Block Panel\">\n <h3 class=\"sub-title1\">References</h3>\n <div\n class=\"ProductDescriptionContainer product-droppdown__section-description\"\n >\n <strong>Background References:</strong>\n <br />\n [1] Shah et al. <em>Mol. Cell</em> (2018). PMID:\n <a\n href=\"https://pubmed.ncbi.nlm.nih.gov/30244833/\"\n target=\"_blank\"\n title=\"Examining the Roles of H3K4 Methylation States with Systematically Characterized Antibodies\"\n >30244833</a\n ><br />\n </div>\n </div>\n </div>\n <div id=\"prodAccordion\">\n <div id=\"ProductDescription\" class=\"Block Panel\">\n <h3 class=\"sub-title1\">Documents & Resources</h3>\n <div\n class=\"ProductDescriptionContainer product-droppdown__section-description\"\n >\n <div class=\"product-documents\">\n <a\n href=\"/content/documents/tds/13-0029.pdf\"\n target=\"_blank\"\n class=\"product-documents__link\"\n >\n <svg\n version=\"1.1\"\n id=\"Layer_1\"\n xmlns=\"http://www.w3.org/2000/svg\"\n xmlns:xlink=\"http://www.w3.org/1999/xlink\"\n x=\"0px\"\n y=\"0px\"\n viewBox=\"0 0 228 240\"\n style=\"enable-background: new 0 0 228 240\"\n xml:space=\"preserve\"\n class=\"product-documents__icon\"\n alt=\"16-0030 Datasheet\"\n >\n <g>\n <path\n class=\"product-documents__svg-pdf\"\n d=\"M191.92,68.77l-47.69-47.69c-1.33-1.33-3.12-2.08-5.01-2.08H45.09C41.17,19,38,22.17,38,26.09v184.36\n c0,3.92,3.17,7.09,7.09,7.09h141.82c3.92,0,7.09-3.17,7.09-7.09V73.8C194,71.92,193.25,70.1,191.92,68.77z M177.65,77.06h-41.7\n v-41.7L177.65,77.06z M178.05,201.59H53.95V34.95h66.92v47.86c0,5.14,4.17,9.31,9.31,9.31h47.86V201.59z\"\n />\n </g>\n <rect\n x=\"20\"\n y=\"112\"\n class=\"product-documents__svg-background\"\n width=\"146\"\n height=\"76\"\n />\n <g>\n <path\n class=\"product-documents__svg-pdf\"\n d=\"M23.83,125.68h22.36c5.29,0,9.41,1.33,12.35,4c2.94,2.67,4.42,6.39,4.42,11.18c0,4.78-1.47,8.51-4.42,11.18\n c-2.94,2.67-7.06,4-12.35,4H34.59v18.29H23.83V125.68z M44.81,147.9c5.38,0,8.07-2.32,8.07-6.97c0-2.39-0.67-4.16-2-5.31\n c-1.33-1.15-3.36-1.73-6.07-1.73H34.59v14.01H44.81z\"\n />\n <path\n class=\"product-documents__svg-pdf\"\n d=\"M69.92,125.68h18.91c5.29,0,9.84,0.97,13.66,2.9c3.82,1.93,6.74,4.72,8.76,8.35\n c2.02,3.63,3.04,7.98,3.04,13.04c0,5.06-1,9.42-3,13.08c-2,3.66-4.91,6.45-8.73,8.38c-3.82,1.93-8.4,2.9-13.73,2.9H69.92V125.68z\n M88.07,165.63c10.35,0,15.52-5.22,15.52-15.66c0-10.4-5.17-15.59-15.52-15.59h-7.38v31.26H88.07z\"\n />\n <path\n class=\"product-documents__svg-pdf\"\n d=\"M122.57,125.68h32.84v8.49h-22.22v11.18h20.84v8.49h-20.84v20.49h-10.63V125.68z\"\n />\n </g>\n </svg>\n <span class=\"product-documents__info\">Technical Datasheet</span>\n </a>\n </div>\n </div>\n </div>\n </div>\n </div>\n</div>\n","detail_messages":"","gift_wrapping_available":false,"gtin":null,"id":"627","images":[{"alt":"Histone H3K9me1 Antibody, SNAP-Certified™ for CUT&RUN and ChIP","data":"https://cdn11.bigcommerce.com/s-y9o92/images/stencil/{:size}/products/627/579/Screen_Shot_2018-09-25_at_2.00.27_PM__31915.1538135192.png?c=2"}],"main_image":{"alt":"Histone H3K9me1 Antibody, SNAP-Certified™ for CUT&RUN and ChIP","data":"https://cdn11.bigcommerce.com/s-y9o92/images/stencil/{:size}/products/627/579/Screen_Shot_2018-09-25_at_2.00.27_PM__31915.1538135192.png?c=2"},"max_purchase_quantity":0,"meta_description":"Rabbit monoclonal histone H3K9me1 antibody rigorously tested for robust and reliable performance in CUT&RUN and ChIP assays. Also validated for ICC/IF & Western Blot","meta_keywords":"H3K9me1 antibody, histone H3K9me1 antibody, histone H3 lysine 9 monomethylated antibody, ChIP antibody, CUT&RUN antibody","min_purchase_quantity":0,"mpn":null,"num_reviews":0,"options":[],"page_title":"H3K9me1 Antibody | SNAP-Certified for CUT&RUN and ChIP","price":{"tax_label":"Sales Tax","without_tax":{"currency":"USD","formatted":"$475.00","value":475}},"rating":0,"related_products":[{"add_to_wishlist_url":"/wishlist.php?action=add&product_id=411","availability":"","brand":{"name":null},"category":["Nucleosomes","Nucleosomes/SNAP-ChIP<sup>®</sup> Spike-ins"],"custom_fields":null,"date_added":"18th Sep 2017","demo":false,"has_options":true,"id":411,"image":{"alt":"SNAP-ChIP® K-MetStat Panel","data":"https://cdn11.bigcommerce.com/s-y9o92/images/stencil/{:size}/products/411/282/SNAP_Chip__53961.1569012494.png?c=2"},"images":[{"alt":"SNAP-ChIP® K-MetStat 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- Type: Monoclonal
- Target Size: 15 kDa
- Format: Affinity Purified IgG
- Host: Rabbit
- Reactivity: Human, Mouse, Wide Range (Predicted)
- Applications: CUT&RUN, ChIP, ICC, WB
Description
This antibody meets EpiCypher’s lot-specific SNAP-Certified™ criteria for specificity and efficient target enrichment in both CUT&RUN and ChIP applications. In CUT&RUN, this requires <20% cross-reactivity to related histone PTMs determined using the SNAP-CUTANA™ K-MetStat Panel of spike-in controls (EpiCypher 19-1002, Figure 1). High target efficiency is confirmed by consistent genomic enrichment at 500k and 50k starting cells (Figures 2-3). In ChIP, this requires <20% cross-reactivity to related histone PTMs and >5% of target input recovered as determined using the SNAP-ChIP® K-MetStat Panel of spike-in controls (EpiCypher 19-1001, Figure 6) [1].
Validation Data
Figure 1: SNAP specificity analysis in CUT&RUN
CUT&RUN was performed on 500k and 50k native K562 cells with
the SNAP-CUTANA K-MetStat Panel (EpiCypher
19-1002) spiked-in prior to the addition of 0.5 µg H3K9me1
antibody. CUT&RUN sequencing reads were aligned to the
unique DNA barcodes corresponding to each nucleosome in the
K-MetStat panel (x-axis). Data are expressed as a percent
relative to on-target recovery (H3K9me1 set to 100%). Error
bars represent mean ± SEM for the duplicate DNA barcodes in
a single CUT&RUN experiment.
Figure 2: Antibody efficiency analysis in CUT&RUN using
cell input correlation
Genome-wide correlation analysis was performed to compare
H3K9me1 antibody enrichment in CUT&RUN using 500k and 50k
cell inputs. The log of the number of reads per 75 bp binned
region across the genome is plotted for both samples.
CUT&RUN data generated using this H3K9me1 antibody are
highly correlated between the two cell inputs (Pearson
correlation r = 0.973), indicating high consistency of
H3K9me1 antibody target recovery.
Figure 3: H3K9me1 CUT&RUN representative tracks at 500k
and 50k cells
Gene browser shots generated using the Integrative Genomics
Viewer (IGV, Broad Institute) show representative loci for
H3K9me1 antibody tested in CUT&RUN using 500k and 50k cells.
Similar results in peak structure and location were observed
for both cell inputs.
Methods: CUT&RUN was performed using the CUTANA™
ChIC/CUT&RUN Kit v2.0 (EpiCypher
14-1048) adapted to high throughput Tecan liquid handling.
Library preparation was performed with 5 ng DNA using the
NEBNext® Ultra™ II DNA Library Prep Kit for
Illumina®. Libraries were run on an Illumina
NextSeq2000 with paired-end sequencing (2x50 bp). Sample
sequencing depth was 4.5 million reads (IgG 50k cell
input), 14.2 million reads (H3K9me1 50k cell input), and
10.1 million reads (H3K9me1 500k cell input).
Figure 4: Immunocytochemistry
ICC of HeLa cells using 2 µg/mL of H3K9me1 antibody (red).
Actin filaments were labeled with fluorescein phalloidin
(green).
Figure 5: Western blot data
Recombinant histone H3.3 (Lane 1) and acid extracts of HeLa
cells (Lane 2) were blotted onto PVDF and probed with 0.5
µg/mL H3K9me1 antibody.
Figure 6: SNAP-ChIP-qPCR specificity and enrichment
analysis
Histone H3K9me1 antibody (3 µg) was tested in a native ChIP
experiment using chromatin from K562 cells (3 µg) with the
SNAP-ChIP K-MetStat Panel (EpiCypher
19-1001) spiked-in prior to micrococcal nuclease digestion.
Specificity (left y-axis) was determined by qPCR for the DNA
barcodes corresponding to modified nucleosomes in the
SNAP-ChIP panel (x-axis). Black bar represents antibody
efficiency (right y-axis; log scale) and indicates
percentage of the target immunoprecipitated relative to
input. Error bars represent mean ± SEM in replicate ChIP
experiments.
Technical Information
Recommended Dilution
CUT&RUN: 0.5 µg per reaction
Chromatin Immunoprecipitation (ChIP): 2 - 5 µg per 5 µg chromatin
Immunofluorescence/Immunocytochemistry (IF/ICC): 0.5 - 2 µg/mL
Western Blot (WB): 0.2 - 1 µg/mL
References
Documents & Resources
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Histone H3K9me1 Antibody, SNAP-Certified™ for CUT&RUN and ChIP
