SKU: 13-0029
{"AddThisServiceButtonMeta":"","add_this":[{"annotation":"","service":"facebook"},{"annotation":"","service":"email"},{"annotation":"","service":"print"},{"annotation":"","service":"twitter"},{"annotation":"","service":"linkedin"}],"add_to_wishlist_url":"/wishlist.php?action=add&product_id=627","availability":"","bulk_discount_rates":[],"can_purchase":true,"cart_url":"https://www.epicypher.com/cart.php","category":["Antibodies/CUTANA™ CUT&RUN Antibodies","Antibodies/CUTANA™ CUT&RUN Antibodies/CUT&RUN Antibodies - Histone PTMs","Antibodies/SNAP-ChIP<sup>®</sup> Certified Antibodies","Epigenetics Kits and Reagents/CUTANA™ ChIC / CUT&RUN Assays"],"custom_fields":[{"id":"453","name":"Pack Size","value":"100 μg"}],"customizations":[],"description":"<div class=\"product-general-info\">\n <ul class=\"product-general-info__list-left\">\n <li class=\"product-general-info__list-item\">\n <strong>Type: </strong>Monoclonal\n </li>\n <li class=\"product-general-info__list-item\">\n <strong>Target Size: </strong>15 kDa\n </li>\n <li class=\"product-general-info__list-item\">\n <strong>Format: </strong>Affinity Purified IgG\n </li>\n </ul>\n <ul class=\"product-general-info__list-right\">\n <li class=\"product-general-info__list-item\">\n <strong>Host: </strong>Rabbit\n </li>\n <li class=\"product-general-info__list-item\">\n <strong>Reactivity: </strong>Human, Mouse, Wide Range\n (Predicted)\n </li>\n <li class=\"product-general-info__list-item\">\n <strong>Applications: </strong>CUT&RUN, ChIP, ICC, WB\n </li>\n </ul>\n</div>\n\n<div class=\"service_accordion product-droppdown\">\n <div class=\"container\">\n <div id=\"prodAccordion\">\n <div id=\"ProductDescription\" class=\"Block Panel current\">\n <h3 class=\"sub-title1\">Description</h3>\n <div\n class=\"ProductDescriptionContainer product-droppdown__section-description-specific\"\n >\n <p>\n This antibody meets EpiCypher’s lot-specific SNAP-Certified™\n criteria for specificity and efficient target enrichment in both\n CUT&RUN and ChIP applications. In CUT&RUN, this requires <20%\n cross-reactivity to related histone PTMs determined using the\n SNAP-CUTANA™ K-MetStat Panel of spike-in controls (EpiCypher\n <a\n href=\"https://www.epicypher.com/products/nucleosomes/snap-cutana-k-metstat-panel\"\n >19-1002</a\n >, <strong>Figure 1</strong>). High target efficiency is confirmed\n by consistent genomic enrichment at 500k and 50k starting cells\n (<strong>Figures 2-3</strong>). In ChIP, this requires <20%\n cross-reactivity to related histone PTMs and >5% of target input\n recovered as determined using the SNAP-ChIP<sup>®</sup> K-MetStat\n Panel of spike-in controls (EpiCypher\n <a\n href=\"https://www.epicypher.com/products/nucleosomes/snap-chip-k-metstat-panel\"\n >19-1001</a\n >, <strong>Figure 6</strong>) [1].\n </p>\n </div>\n </div>\n </div>\n <div id=\"prodAccordion\">\n <div id=\"ProductDescription\" class=\"Block Panel current\">\n <h3 class=\"sub-title1\">Validation Data</h3>\n <div\n class=\"ProductDescriptionContainer product-droppdown__section-description-specific\"\n >\n <section class=\"image-picker\">\n <div class=\"image-picker__left\">\n <div\n class=\"image-picker__main-content_active image-picker__main-content\"\n >\n <div class=\"image-picker__header-content\">\n <button class=\"image-picker__left-arrow\">\n <svg\n class=\"image-picker__svg-left\"\n width=\"24\"\n height=\"24\"\n viewBox=\"0 0 24 24\"\n >\n <path\n d=\"M16.67 0l2.83 2.829-9.339 9.175 9.339 9.167-2.83 2.829-12.17-11.996z\"\n />\n </svg>\n </button>\n <a\n href=\"/content/images/products/antibodies/13-0029-specificity-analysis.jpeg\"\n target=\"_blank\"\n class=\"image-picker__main-image-link\"\n ><img\n alt=\"13-0029-specificity-analysis\"\n src=\"/content/images/products/antibodies/13-0029-specificity-analysis.jpeg\"\n class=\"image-picker__main-image\"\n />\n <span class=\"image-picker__main-image-caption\"\n >(Click to enlarge)</span\n ></a\n >\n <button class=\"image-picker__right-arrow\">\n <svg\n class=\"image-picker__svg-right\"\n width=\"24\"\n height=\"24\"\n viewBox=\"0 0 24 24\"\n >\n <path\n d=\"M7.33 24l-2.83-2.829 9.339-9.175-9.339-9.167 2.83-2.829 12.17 11.996z\"\n />\n </svg>\n </button>\n </div>\n <p>\n <span class=\"image-picker__span-content\"\n ><strong\n >Figure 1: SNAP specificity analysis in CUT&RUN </strong\n ><br />\n CUT&RUN was performed on 500k and 50k native K562 cells with\n the SNAP-CUTANA K-MetStat Panel (EpiCypher\n <a\n href=\"https://www.epicypher.com/products/nucleosomes/snap-cutana-k-metstat-panel\"\n >19-1002</a\n >) spiked-in prior to the addition of 0.5 µg H3K9me1\n antibody. CUT&RUN sequencing reads were aligned to the\n unique DNA barcodes corresponding to each nucleosome in the\n K-MetStat panel (x-axis). Data are expressed as a percent\n relative to on-target recovery (H3K9me1 set to 100%). Error\n bars represent mean ± SEM for the duplicate DNA barcodes in\n a single CUT&RUN experiment.\n </span>\n </p>\n </div>\n <div class=\"image-picker__main-content\">\n <div class=\"image-picker__header-content\">\n <button class=\"image-picker__left-arrow\">\n <svg\n class=\"image-picker__svg-left\"\n width=\"24\"\n height=\"24\"\n viewBox=\"0 0 24 24\"\n >\n <path\n d=\"M16.67 0l2.83 2.829-9.339 9.175 9.339 9.167-2.83 2.829-12.17-11.996z\"\n />\n </svg>\n </button>\n <a\n href=\"/content/images/products/antibodies/13-0029-efficiency-analysis.jpeg\"\n target=\"_blank\"\n class=\"image-picker__main-image-link\"\n ><img\n alt=\"13-0029-efficiency-analysis\"\n src=\"/content/images/products/antibodies/13-0029-efficiency-analysis.jpeg\"\n class=\"image-picker__main-image\"\n />\n <span class=\"image-picker__main-image-caption\"\n >(Click to enlarge)</span\n ></a\n >\n <button class=\"image-picker__right-arrow\">\n <svg\n class=\"image-picker__svg-right\"\n width=\"24\"\n height=\"24\"\n viewBox=\"0 0 24 24\"\n >\n <path\n d=\"M7.33 24l-2.83-2.829 9.339-9.175-9.339-9.167 2.83-2.829 12.17 11.996z\"\n />\n </svg>\n </button>\n </div>\n <p>\n <span class=\"image-picker__span-content\"\n ><strong\n >Figure 2: Antibody efficiency analysis in CUT&RUN using\n cell input correlation </strong\n ><br />\n Genome-wide correlation analysis was performed to compare\n H3K9me1 antibody enrichment in CUT&RUN using 500k and 50k\n cell inputs. The log of the number of reads per 75 bp binned\n region across the genome is plotted for both samples.\n CUT&RUN data generated using this H3K9me1 antibody are\n highly correlated between the two cell inputs (Pearson\n correlation r = 0.973), indicating high consistency of\n H3K9me1 antibody target recovery.\n </span>\n </p>\n </div>\n <div class=\"image-picker__main-content\">\n <div class=\"image-picker__header-content\">\n <button class=\"image-picker__left-arrow\">\n <svg\n class=\"image-picker__svg-left\"\n width=\"24\"\n height=\"24\"\n viewBox=\"0 0 24 24\"\n >\n <path\n d=\"M16.67 0l2.83 2.829-9.339 9.175 9.339 9.167-2.83 2.829-12.17-11.996z\"\n />\n </svg>\n </button>\n <a\n href=\"/content/images/products/antibodies/13-0029-representative-tracks.jpeg\"\n target=\"_blank\"\n class=\"image-picker__main-image-link\"\n >\n <img\n alt=\"13-0029-representative-tracks\"\n src=\"/content/images/products/antibodies/13-0029-representative-tracks.jpeg\"\n class=\"image-picker__main-image\"\n />\n <span class=\"image-picker__main-image-caption\"\n >(Click to enlarge)</span\n >\n </a>\n <button class=\"image-picker__right-arrow\">\n <svg\n class=\"image-picker__svg-right\"\n width=\"24\"\n height=\"24\"\n viewBox=\"0 0 24 24\"\n >\n <path\n d=\"M7.33 24l-2.83-2.829 9.339-9.175-9.339-9.167 2.83-2.829 12.17 11.996z\"\n />\n </svg>\n </button>\n </div>\n <p>\n <span class=\"image-picker__span-content\">\n <strong\n >Figure 3: H3K9me1 CUT&RUN representative tracks at 500k\n and 50k cells </strong\n ><br />\n Gene browser shots generated using the Integrative Genomics\n Viewer (IGV, Broad Institute) show representative loci for\n H3K9me1 antibody tested in CUT&RUN using 500k and 50k cells.\n Similar results in peak structure and location were observed\n for both cell inputs.\n <em\n ><u>Methods:</u> CUT&RUN was performed using the CUTANA™\n ChIC/CUT&RUN Kit v2.0 (EpiCypher\n <a\n href=\"https://www.epicypher.com/products/epigenetics-reagents-and-assays/cutana-chic-cut-and-run-kit\"\n >14-1048</a\n >) adapted to high throughput Tecan liquid handling.\n Library preparation was performed with 5 ng DNA using the\n NEBNext<sup>®</sup> Ultra™ II DNA Library Prep Kit for\n Illumina<sup>®</sup>. Libraries were run on an Illumina\n NextSeq2000 with paired-end sequencing (2x50 bp). Sample\n sequencing depth was 4.5 million reads (IgG 50k cell\n input), 14.2 million reads (H3K9me1 50k cell input), and\n 10.1 million reads (H3K9me1 500k cell input).</em\n >\n </span>\n </p>\n </div>\n <div class=\"image-picker__main-content\">\n <div class=\"image-picker__header-content\">\n <button class=\"image-picker__left-arrow\">\n <svg\n class=\"image-picker__svg-left\"\n width=\"24\"\n height=\"24\"\n viewBox=\"0 0 24 24\"\n >\n <path\n d=\"M16.67 0l2.83 2.829-9.339 9.175 9.339 9.167-2.83 2.829-12.17-11.996z\"\n />\n </svg>\n </button>\n <a\n href=\"/content/images/products/antibodies/13-0029-immunocytochemistry.jpeg\"\n target=\"_blank\"\n class=\"image-picker__main-image-link\"\n >\n <img\n alt=\"13-0029-immunocytochemistry\"\n src=\"/content/images/products/antibodies/13-0029-immunocytochemistry.jpeg\"\n class=\"image-picker__main-image\"\n />\n <span class=\"image-picker__main-image-caption\"\n >(Click to enlarge)</span\n >\n </a>\n <button class=\"image-picker__right-arrow\">\n <svg\n class=\"image-picker__svg-right\"\n width=\"24\"\n height=\"24\"\n viewBox=\"0 0 24 24\"\n >\n <path\n d=\"M7.33 24l-2.83-2.829 9.339-9.175-9.339-9.167 2.83-2.829 12.17 11.996z\"\n />\n </svg>\n </button>\n </div>\n <p>\n <span class=\"image-picker__span-content\">\n <strong>Figure 4: Immunocytochemistry </strong><br />\n ICC of HeLa cells using 2 µg/mL of H3K9me1 antibody (red).\n Actin filaments were labeled with fluorescein phalloidin\n (green).\n </span>\n </p>\n </div>\n <div class=\"image-picker__main-content\">\n <div class=\"image-picker__header-content\">\n <button class=\"image-picker__left-arrow\">\n <svg\n class=\"image-picker__svg-left\"\n width=\"24\"\n height=\"24\"\n viewBox=\"0 0 24 24\"\n >\n <path\n d=\"M16.67 0l2.83 2.829-9.339 9.175 9.339 9.167-2.83 2.829-12.17-11.996z\"\n />\n </svg>\n </button>\n <a\n href=\"/content/images/products/antibodies/13-0029-western-blot-data.jpeg\"\n target=\"_blank\"\n class=\"image-picker__main-image-link\"\n >\n <img\n alt=\"13-0029-western-blot-data\"\n src=\"/content/images/products/antibodies/13-0029-western-blot-data.jpeg\"\n class=\"image-picker__main-image\"\n />\n <span class=\"image-picker__main-image-caption\"\n >(Click to enlarge)</span\n >\n </a>\n <button class=\"image-picker__right-arrow\">\n <svg\n class=\"image-picker__svg-right\"\n width=\"24\"\n height=\"24\"\n viewBox=\"0 0 24 24\"\n >\n <path\n d=\"M7.33 24l-2.83-2.829 9.339-9.175-9.339-9.167 2.83-2.829 12.17 11.996z\"\n />\n </svg>\n </button>\n </div>\n <p>\n <span class=\"image-picker__span-content\">\n <strong>Figure 5: Western blot data </strong><br />\n Recombinant histone H3.3 (Lane 1) and acid extracts of HeLa\n cells (Lane 2) were blotted onto PVDF and probed with 0.5\n µg/mL H3K9me1 antibody.\n </span>\n </p>\n </div>\n <div class=\"image-picker__main-content\">\n <div class=\"image-picker__header-content\">\n <button class=\"image-picker__left-arrow\">\n <svg\n class=\"image-picker__svg-left\"\n width=\"24\"\n height=\"24\"\n viewBox=\"0 0 24 24\"\n >\n <path\n d=\"M16.67 0l2.83 2.829-9.339 9.175 9.339 9.167-2.83 2.829-12.17-11.996z\"\n />\n </svg>\n </button>\n <a\n href=\"/content/images/products/antibodies/13-0029-specificity-and-enrichment-analysis.jpeg\"\n target=\"_blank\"\n class=\"image-picker__main-image-link\"\n >\n <img\n alt=\"13-0029-specificity-and-enrichment-analysis\"\n src=\"/content/images/products/antibodies/13-0029-specificity-and-enrichment-analysis.jpeg\"\n class=\"image-picker__main-image\"\n />\n <span class=\"image-picker__main-image-caption\"\n >(Click to enlarge)</span\n >\n </a>\n <button class=\"image-picker__right-arrow\">\n <svg\n class=\"image-picker__svg-right\"\n width=\"24\"\n height=\"24\"\n viewBox=\"0 0 24 24\"\n >\n <path\n d=\"M7.33 24l-2.83-2.829 9.339-9.175-9.339-9.167 2.83-2.829 12.17 11.996z\"\n />\n </svg>\n </button>\n </div>\n <p>\n <span class=\"image-picker__span-content\">\n <strong\n >Figure 6: SNAP-ChIP-qPCR specificity and enrichment\n analysis </strong\n ><br />\n Histone H3K9me1 antibody (3 µg) was tested in a native ChIP\n experiment using chromatin from K562 cells (3 µg) with the\n SNAP-ChIP K-MetStat Panel (EpiCypher\n <a\n href=\"https://www.epicypher.com/products/nucleosomes/snap-chip-k-metstat-panel\"\n >19-1001</a\n >) spiked-in prior to micrococcal nuclease digestion.\n Specificity (left y-axis) was determined by qPCR for the DNA\n barcodes corresponding to modified nucleosomes in the\n SNAP-ChIP panel (x-axis). Black bar represents antibody\n efficiency (right y-axis; log scale) and indicates\n percentage of the target immunoprecipitated relative to\n input. Error bars represent mean ± SEM in replicate ChIP\n experiments.\n </span>\n </p>\n </div>\n </div>\n <aside class=\"image-picker__right\">\n <div class=\"image-picker__gallery\">\n <img\n alt=\"13-0029-specificity-analysis\"\n src=\"/content/images/products/antibodies/13-0029-specificity-analysis.jpeg\"\n width=\"200\"\n class=\"image-picker__side-image image-picker__side-image_active\"\n role=\"button\"\n />\n <img\n alt=\"13-0029-efficiency-analysis\"\n src=\"/content/images/products/antibodies/13-0029-efficiency-analysis.jpeg\"\n class=\"image-picker__side-image\"\n role=\"button\"\n />\n <img\n alt=\"13-0029-representative-tracks\"\n src=\"/content/images/products/antibodies/13-0029-representative-tracks.jpeg\"\n class=\"image-picker__side-image\"\n role=\"button\"\n />\n <img\n alt=\"13-0029-immunocytochemistry\"\n src=\"/content/images/products/antibodies/13-0029-immunocytochemistry.jpeg\"\n class=\"image-picker__side-image\"\n role=\"button\"\n />\n <img\n alt=\"13-0029-western-blot-data\"\n src=\"/content/images/products/antibodies/13-0029-western-blot-data.jpeg\"\n class=\"image-picker__side-image\"\n role=\"button\"\n />\n <img\n alt=\"13-0029-specificity-and-enrichment-analysis\"\n src=\"/content/images/products/antibodies/13-0029-specificity-and-enrichment-analysis.jpeg\"\n class=\"image-picker__side-image\"\n role=\"button\"\n />\n </div>\n </aside>\n </section>\n </div>\n </div>\n </div>\n <div id=\"prodAccordion\">\n <div id=\"ProductDescription\" class=\"Block Panel\">\n <h3 class=\"sub-title1\">Technical Information</h3>\n <div\n class=\"ProductDescriptionContainer product-droppdown__section-description\"\n >\n <div class=\"product-tech-info\">\n <div class=\"product-tech-info__line-item\">\n <div class=\"product-tech-info__line-item-left\">\n <b>Immunogen</b>\n </div>\n <div class=\"product-tech-info__line-item-right\">\n A synthetic peptide corresponding to histone H3 monomethylated\n at lysine 9.\n </div>\n </div>\n <div class=\"product-tech-info__line-item\">\n <div class=\"product-tech-info__line-item-left\">\n <b>Formulation</b>\n </div>\n <div class=\"product-tech-info__line-item-right\">\n Protein A affinity-purified antibody in PBS, with 0.09% sodium\n azide, 1% BSA, and 50% glycerol.\n </div>\n </div>\n <div class=\"product-tech-info__line-item\">\n <div class=\"product-tech-info__line-item-left\">\n <b>Storage and Stability</b>\n </div>\n <div class=\"product-tech-info__line-item-right\">\n Stable for 1 year at -20°C from date of receipt.\n </div>\n </div>\n </div>\n </div>\n </div>\n </div>\n <div id=\"prodAccordion\">\n <div id=\"ProductDescription\" class=\"Block Panel\">\n <h3 class=\"sub-title1\">Recommended Dilution</h3>\n <div\n class=\"ProductDescriptionContainer product-droppdown__section-description\"\n >\n <p><strong>CUT&RUN:</strong> 0.5 µg per reaction</p>\n <p>\n <strong>Chromatin Immunoprecipitation (ChIP):</strong> 2 - 5 µg per\n 5 µg chromatin\n </p>\n <p>\n <strong>Immunofluorescence/Immunocytochemistry (IF/ICC):</strong>\n 0.5 - 2 µg/mL\n </p>\n <p><strong>Western Blot (WB):</strong> 0.2 - 1 µg/mL</p>\n </div>\n </div>\n </div>\n <div id=\"prodAccordion\">\n <div id=\"ProductDescription\" class=\"Block Panel\">\n <h3 class=\"sub-title1\">References</h3>\n <div\n class=\"ProductDescriptionContainer product-droppdown__section-description\"\n >\n <strong>Background References:</strong>\n <br />\n [1] Shah et al. <em>Mol. Cell</em> (2018). PMID:\n <a\n href=\"https://pubmed.ncbi.nlm.nih.gov/30244833/\"\n target=\"_blank\"\n title=\"Examining the Roles of H3K4 Methylation States with Systematically Characterized Antibodies\"\n >30244833</a\n ><br />\n </div>\n </div>\n </div>\n <div id=\"prodAccordion\">\n <div id=\"ProductDescription\" class=\"Block Panel\">\n <h3 class=\"sub-title1\">Documents & Resources</h3>\n <div\n class=\"ProductDescriptionContainer product-droppdown__section-description\"\n >\n <div class=\"product-documents\">\n <a\n href=\"/content/documents/tds/13-0029.pdf\"\n target=\"_blank\"\n class=\"product-documents__link\"\n >\n <svg\n version=\"1.1\"\n id=\"Layer_1\"\n xmlns=\"http://www.w3.org/2000/svg\"\n xmlns:xlink=\"http://www.w3.org/1999/xlink\"\n x=\"0px\"\n y=\"0px\"\n viewBox=\"0 0 228 240\"\n style=\"enable-background: new 0 0 228 240\"\n xml:space=\"preserve\"\n class=\"product-documents__icon\"\n alt=\"16-0030 Datasheet\"\n >\n <g>\n <path\n class=\"product-documents__svg-pdf\"\n d=\"M191.92,68.77l-47.69-47.69c-1.33-1.33-3.12-2.08-5.01-2.08H45.09C41.17,19,38,22.17,38,26.09v184.36\n c0,3.92,3.17,7.09,7.09,7.09h141.82c3.92,0,7.09-3.17,7.09-7.09V73.8C194,71.92,193.25,70.1,191.92,68.77z M177.65,77.06h-41.7\n v-41.7L177.65,77.06z M178.05,201.59H53.95V34.95h66.92v47.86c0,5.14,4.17,9.31,9.31,9.31h47.86V201.59z\"\n />\n </g>\n <rect\n x=\"20\"\n y=\"112\"\n class=\"product-documents__svg-background\"\n width=\"146\"\n height=\"76\"\n />\n <g>\n <path\n class=\"product-documents__svg-pdf\"\n d=\"M23.83,125.68h22.36c5.29,0,9.41,1.33,12.35,4c2.94,2.67,4.42,6.39,4.42,11.18c0,4.78-1.47,8.51-4.42,11.18\n c-2.94,2.67-7.06,4-12.35,4H34.59v18.29H23.83V125.68z M44.81,147.9c5.38,0,8.07-2.32,8.07-6.97c0-2.39-0.67-4.16-2-5.31\n c-1.33-1.15-3.36-1.73-6.07-1.73H34.59v14.01H44.81z\"\n />\n <path\n class=\"product-documents__svg-pdf\"\n d=\"M69.92,125.68h18.91c5.29,0,9.84,0.97,13.66,2.9c3.82,1.93,6.74,4.72,8.76,8.35\n c2.02,3.63,3.04,7.98,3.04,13.04c0,5.06-1,9.42-3,13.08c-2,3.66-4.91,6.45-8.73,8.38c-3.82,1.93-8.4,2.9-13.73,2.9H69.92V125.68z\n M88.07,165.63c10.35,0,15.52-5.22,15.52-15.66c0-10.4-5.17-15.59-15.52-15.59h-7.38v31.26H88.07z\"\n />\n <path\n class=\"product-documents__svg-pdf\"\n d=\"M122.57,125.68h32.84v8.49h-22.22v11.18h20.84v8.49h-20.84v20.49h-10.63V125.68z\"\n />\n </g>\n </svg>\n <span class=\"product-documents__info\">Technical Datasheet</span>\n </a>\n </div>\n </div>\n </div>\n </div>\n </div>\n</div>\n","detail_messages":"","gift_wrapping_available":false,"gtin":null,"id":"627","images":[{"alt":"Histone H3K9me1 Antibody, SNAP-Certified™ for CUT&RUN and ChIP","data":"https://cdn11.bigcommerce.com/s-y9o92/images/stencil/{:size}/products/627/579/Screen_Shot_2018-09-25_at_2.00.27_PM__31915.1538135192.png?c=2"}],"main_image":{"alt":"Histone H3K9me1 Antibody, SNAP-Certified™ for CUT&RUN and ChIP","data":"https://cdn11.bigcommerce.com/s-y9o92/images/stencil/{:size}/products/627/579/Screen_Shot_2018-09-25_at_2.00.27_PM__31915.1538135192.png?c=2"},"max_purchase_quantity":0,"meta_description":"Rabbit monoclonal histone H3K9me1 antibody rigorously tested for robust and reliable performance in CUT&RUN and ChIP assays. 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Pack Size: 100 μg
