dCypher® Nucleosome K-MetStat & OncoStat Panel
dCypher Panels Frequently Asked Questions
Q: How many assay points of each nucleosome are supplied?
Q: How do I store the plate? Can I dilute the nucleosomes?
For applications that may not be compatible with the SNAP-ChIP buffer, EpiCypher routinely dilutes nucleosomes to 40 nM in assay buffer (50 mM Tris pH 7.5, 0.01% Tween-20, 0.01% BSA). Under these conditions the nucleosomes are stable for ~2 weeks at -20ºC.
*Note: extremely high amounts of salt (>650 mM) and ionic detergents (e.g. SDS) will disrupt nucleosome stability.