CUTANA™ CUT&Tag Core Reagents Bundle *DISCONTINUED*
{"url":"https://www.epicypher.com/products/epigenetics-kits-and-reagents/cutana-cut-tag-core-reagents-bundle","add_this":[{"service":"facebook","annotation":""},{"service":"email","annotation":""},{"service":"print","annotation":""},{"service":"twitter","annotation":""},{"service":"linkedin","annotation":""}],"gtin":"","max_purchase_quantity":0,"id":"890","bulk_discount_rates":[],"can_purchase":false,"meta_description":"This bundle brings together many of the essential components of a CUT&Tag kit, offering a significant cost savings over purchasing each individual reagent.","category":[],"meta_keywords":"CUT&Tag, cut and tag, cutandtag, Cleavage Under Targets and Tagmentation, CUT&Tag enzyme, CUT&Tag assay, CUT&Tag kit","AddThisServiceButtonMeta":"","images":[{"data":"https://cdn11.bigcommerce.com/s-y9o92/images/stencil/{:size}/products/890/918/2020_CUTTag_icon_RGB_Andy_L__93238.1592491196__35422.1643742241.png?c=2","alt":"CUTANA™ CUT&Tag Core Reagents Bundle *DISCONTINUED*"}],"main_image":{"data":"https://cdn11.bigcommerce.com/s-y9o92/images/stencil/{:size}/products/890/918/2020_CUTTag_icon_RGB_Andy_L__93238.1592491196__35422.1643742241.png?c=2","alt":"CUTANA™ CUT&Tag Core Reagents Bundle *DISCONTINUED*"},"add_to_wishlist_url":"/wishlist.php?action=add&product_id=890","shipping":[],"num_reviews":0,"weight":"0.01 LBS","custom_fields":[{"id":"984","name":"Pack Size","value":"50 Reactions"}],"sku":"14-1101","description":"<a\n style=\"color: #fff\"\n href=\"/products/epigenetics-kits-and-reagents/cutana-cut-and-tag-kit\">\n <p\n style=\"\n background-color: #4698cb;\n color: #fff;\n padding: 1.3rem;\n text-align: center;\n border-radius: 12px;\n margin-top: 2.5rem;\n \">\n Product Discontinued - See 14-1102 and 14-1103 as recommended alternatives\n </p>\n</a>\n<div class=\"service_accordion product-droppdown\">\n <div class=\"container\">\n <div id=\"prodAccordion\">\n <div id=\"ProductDescription\" class=\"Block Panel current\">\n <h3 class=\"sub-title1\">Description</h3>\n <div\n class=\"ProductDescriptionContainer product-droppdown__section-description-specific\">\n <p>\n The CUTANA™ CUT&Tag Core Reagents Bundle brings together the\n essential components of a CUT&Tag kit, offering a significant cost\n savings over purchasing each individual reagent. Detailed\n instructions for using the bundled reagents can be found in the\n EpiCypher\n <a href=\"/content/documents/protocols/cutana-cut&tag-protocol.pdf\"\n >CUT&Tag protocol</a\n >. Included are ConA magnetic beads for immobilizing nuclei or\n cells, control antibodies, anti-rabbit secondary antibody, spike-in\n controls, pAG-Tn5 for antibody-targeted chromatin tagmentation, and\n PCR master mix for library amplification. The positive and negative\n control antibodies (H3K4me3, IgG) can be paired with the\n SNAP-CUTANA™ K-MetStat Panel of spike-in controls for assay\n optimization and reaction monitoring.\n </p>\n <p>\n Some necessary reagents including CUT&Tag assay buffers, primers,\n and DNA purification beads are not included in the bundle (see\n <strong>Required Materials Not Supplied</strong>). For detailed\n buffer recipes, multiplexing primer sequences and recommended\n vendors, see the\n <a\n href=\"https://www.epicypher.com/content/documents/protocols/cutana-cut&tag-protocol.pdf\"\n >CUT&Tag protocol</a\n >.\n </p>\n\n <p>\n The EpiCypher\n <a\n href=\"https://www.epicypher.com/content/documents/protocols/cutana-cut&tag-protocol.pdf\"\n >CUT&Tag protocol</a\n >\n is compatible with a variety of inputs, though nuclei are preferred\n to reduce mitochondrial background. While it is recommended to start\n with 100,000 cells, comparable data can be generated using as few as\n 1,000 cells. CUT&Tag provides robust results when profiling histone\n post-translational modifications (PTMs), but is not recommended for\n chromatin associated proteins (CAPs). The inclusion of controls, low\n cell tolerance, and robust PTM profiling potential make the CUT&Tag\n bundle the ideal choice for experienced users looking for high\n throughput chromatin mapping. For new users, projects mapping CAPs,\n or projects mapping histone PTMs where >5,000 cells / sample can be\n attained, check out the CUTANA™ ChIC/CUT&RUN Kit (EpiCypher\n <a\n href=\"/products/epigenetics-reagents-and-assays/cutana-chic-cut-and-run-kit\"\n >14-1048</a\n >) for an all-inclusive and robust epigenomic profiling solution.\n </p>\n </div>\n </div>\n </div>\n <div id=\"prodAccordion\">\n <div id=\"ProductDescription\" class=\"Block Panel current\">\n <h3 class=\"sub-title1\">Bundle Contents</h3>\n <div\n class=\"ProductDescriptionContainer product-droppdown__section-description-specific\">\n <table>\n <tr>\n <th>Item</th>\n <th>Pack Size</th>\n <th>CAT</th>\n </tr>\n\n <tr>\n <td>pAG-Tn5</td>\n <td>50 Reactions</td>\n <td>\n <a\n href=\"https://www.epicypher.com/products/featured-products/cutana-pag-tn5-for-cut-tag-50-rxns\"\n >15-1017</a\n >\n </td>\n </tr>\n\n <tr>\n <td>Concanavalin A Beads</td>\n <td>50 Reactions</td>\n <td>\n <a\n href=\"https://www.epicypher.com/products/epigenetics-reagents-and-assays/cutana-chic-cut-run-assays/cutana-concanavalin-a-conjugated-paramagnetic-beads\"\n >21-1401</a\n >\n </td>\n </tr>\n\n <tr>\n <td>SNAP-CUTANA™ K-MetStat Panel</td>\n <td>20 Reactions</td>\n <td>\n <a\n href=\"https://www.epicypher.com/products/nucleosomes/snap-cutana-k-metstat-panel\"\n >19-1002t</a\n >\n </td>\n </tr>\n\n <tr>\n <td>H3K4me3 Control Antibody</td>\n <td>10 Reactions</td>\n <td>\n <a\n href=\"https://www.epicypher.com/products/antibodies/snap-chip-certified-antibodies/histone-h3k4me3-antibody-snap-chip-certified-cutana-cut-run-compatible\"\n >13-0041t</a\n >\n </td>\n </tr>\n\n <tr>\n <td>Rabbit IgG Control Antibody</td>\n <td>10 Reactions</td>\n <td>\n <a\n href=\"https://www.epicypher.com/products/nucleosomes/snap-cutana-spike-in-controls/cutana-rabbit-igg-cut-run-negative-control-antibody\"\n >13-0042t</a\n >\n </td>\n </tr>\n\n <tr>\n <td>Anti-rabbit secondary</td>\n <td>50 Reactions</td>\n <td>\n <a\n href=\"https://www.epicypher.com/products/epigenetics-reagents-and-assays/cutana-cut-tag-assays/anti-rabbit-secondary-antibody-for-cutana-cut-tag-workflows\"\n >13-0047</a\n >\n </td>\n </tr>\n\n <tr>\n <td>High Fidelity 2X PCR Master Mix</td>\n <td>50 Reactions</td>\n <td>\n <a\n href=\"https://www.epicypher.com/products/epigenetics-reagents-and-assays/cutana-chic-cut-tag-assays/cutana-high-fidelity-2x-pcr-master-mix\"\n >15-1018</a\n >\n </td>\n </tr>\n </table>\n </div>\n </div>\n </div>\n <div id=\"prodAccordion\">\n <div id=\"ProductDescription\" class=\"Block Panel\">\n <h3 class=\"sub-title1\">Validation Data</h3>\n <div\n class=\"ProductDescriptionContainer product-droppdown__section-description\">\n <p>\n For product-specific validation data, see the individual product\n pages linked above\n </p>\n </div>\n </div>\n </div>\n <div id=\"prodAccordion\">\n <div id=\"ProductDescription\" class=\"Block Panel\">\n <h3 class=\"sub-title1\">Required Materials Not Supplied</h3>\n <div\n class=\"ProductDescriptionContainer product-droppdown__section-description\">\n <p>\n The following reagents are not included. See our\n <a\n href=\"https://www.epicypher.com/content/documents/protocols/cutana-cut&tag-protocol.pdf\"\n >CUT&Tag protocol</a\n >\n for more information:\n </p>\n <ul>\n <li style=\"margin-bottom: 0\">Assay buffers</li>\n <li style=\"margin-bottom: 0\">\n Multiplexing primers (requires custom oligo synthesis)\n </li>\n <li style=\"margin-bottom: 0\">\n SPRI Beads (e.g. AMPure XP, SPRIselect)\n </li>\n <li style=\"margin-bottom: 0\">8-strip tubes & magnets</li>\n <li style=\"margin-bottom: 0\">\n Qubit™ 4 Fluorometer & 1X dsDNA HS Kit\n </li>\n <li style=\"margin-bottom: 1rem !important\">\n Agilent BioAnalyzer<sup>®</sup> or TapeStation<sup>®</sup>\n </li>\n <li>\n <strong>If using a mouse primary antibody</strong>, the anti-mouse\n secondary antibody (EpiCypher\n <a\n href=\"/products/epigenetics-reagents-and-assays/cutanac-cut-tag-assays/anti-mouse-secondary-antibody-for-cutana-cut-tag-workflows\"\n >13-0048</a\n >) must be purchased separately.\n </li>\n </ul>\n </div>\n </div>\n </div>\n <div id=\"prodAccordion\">\n <div id=\"ProductDescription\" class=\"Block Panel\">\n <h3 class=\"sub-title1\">Technical Information</h3>\n <div\n class=\"ProductDescriptionContainer product-droppdown__section-description\">\n <div class=\"product-tech-info\">\n <div class=\"product-tech-info__line-item\">\n <div class=\"product-tech-info__line-item-left\">\n <b>Storage and Stability</b>\n </div>\n <div class=\"product-tech-info__line-item-right\">\n DO NOT FREEZE ConA BEADS. Upon receipt, remove Concanavalin A\n (ConA) Beads and store at 4°C. Store the remainder of the\n components at -20°C. Stability from date of receipt as\n indicated on each reagent.\n </div>\n </div>\n <div class=\"product-tech-info__line-item\">\n <div class=\"product-tech-info__line-item-left\">\n <b>Instructions for use</b>\n </div>\n <div class=\"product-tech-info__line-item-right\">\n For additional required buffers, materials, and equipment see\n our\n <a\n href=\"https://www.epicypher.com/content/documents/protocols/cutana-cut&tag-protocol.pdf\"\n >CUT&Tag protocol</a\n >. Use 0.5 µg per CUT&Tag reaction for each control antibody.\n </div>\n </div>\n </div>\n </div>\n </div>\n </div>\n <div id=\"prodAccordion\">\n <div id=\"ProductDescription\" class=\"Block Panel\">\n <h3 class=\"sub-title1\">Additional Information</h3>\n <div\n class=\"ProductDescriptionContainer product-droppdown__section-description\">\n <p>\n Beckman Coulter, the stylized logo, AMPure XP, and SPRIselect are\n trademarks or registered trademarks of Beckman Coulter, Inc. in the\n United States and other countries.\n </p>\n </div>\n </div>\n </div>\n <div id=\"prodAccordion\">\n <div id=\"ProductDescription\" class=\"Block Panel\">\n <h3 class=\"sub-title1\">Documents & Resources</h3>\n <div\n class=\"ProductDescriptionContainer product-droppdown__section-description\">\n <div class=\"product-documents\">\n <a\n href=\"/content/documents/tds/14-1101.pdf\"\n target=\"_new\"\n class=\"product-documents__link\">\n <svg\n version=\"1.1\"\n id=\"Layer_1\"\n xmlns=\"http://www.w3.org/2000/svg\"\n xmlns:xlink=\"http://www.w3.org/1999/xlink\"\n x=\"0px\"\n y=\"0px\"\n viewBox=\"0 0 228 240\"\n style=\"enable-background: new 0 0 228 240\"\n xml:space=\"preserve\"\n class=\"product-documents__icon\"\n alt=\"16-0002 Datasheet\">\n <g>\n <path\n class=\"product-documents__svg-pdf\"\n d=\"M191.92,68.77l-47.69-47.69c-1.33-1.33-3.12-2.08-5.01-2.08H45.09C41.17,19,38,22.17,38,26.09v184.36\n c0,3.92,3.17,7.09,7.09,7.09h141.82c3.92,0,7.09-3.17,7.09-7.09V73.8C194,71.92,193.25,70.1,191.92,68.77z M177.65,77.06h-41.7\n v-41.7L177.65,77.06z M178.05,201.59H53.95V34.95h66.92v47.86c0,5.14,4.17,9.31,9.31,9.31h47.86V201.59z\" />\n </g>\n <rect\n x=\"20\"\n y=\"112\"\n class=\"product-documents__svg-background\"\n width=\"146\"\n height=\"76\" />\n <g>\n <path\n class=\"product-documents__svg-pdf\"\n d=\"M23.83,125.68h22.36c5.29,0,9.41,1.33,12.35,4c2.94,2.67,4.42,6.39,4.42,11.18c0,4.78-1.47,8.51-4.42,11.18\n c-2.94,2.67-7.06,4-12.35,4H34.59v18.29H23.83V125.68z M44.81,147.9c5.38,0,8.07-2.32,8.07-6.97c0-2.39-0.67-4.16-2-5.31\n c-1.33-1.15-3.36-1.73-6.07-1.73H34.59v14.01H44.81z\" />\n <path\n class=\"product-documents__svg-pdf\"\n d=\"M69.92,125.68h18.91c5.29,0,9.84,0.97,13.66,2.9c3.82,1.93,6.74,4.72,8.76,8.35\n c2.02,3.63,3.04,7.98,3.04,13.04c0,5.06-1,9.42-3,13.08c-2,3.66-4.91,6.45-8.73,8.38c-3.82,1.93-8.4,2.9-13.73,2.9H69.92V125.68z\n M88.07,165.63c10.35,0,15.52-5.22,15.52-15.66c0-10.4-5.17-15.59-15.52-15.59h-7.38v31.26H88.07z\" />\n <path\n class=\"product-documents__svg-pdf\"\n d=\"M122.57,125.68h32.84v8.49h-22.22v11.18h20.84v8.49h-20.84v20.49h-10.63V125.68z\" />\n </g>\n </svg>\n <span class=\"product-documents__info\">Technical Datasheet</span>\n </a>\n </div>\n <div class=\"product-documents\">\n <a\n href=\"/content/documents/protocols/cutana-cut&tag-protocol.pdf\"\n target=\"_new\"\n class=\"product-documents__link\">\n <svg\n version=\"1.1\"\n id=\"Layer_1\"\n xmlns=\"http://www.w3.org/2000/svg\"\n xmlns:xlink=\"http://www.w3.org/1999/xlink\"\n x=\"0px\"\n y=\"0px\"\n viewBox=\"0 0 228 240\"\n style=\"enable-background: new 0 0 228 240\"\n xml:space=\"preserve\"\n class=\"product-documents__icon\"\n alt=\"16-0002 Datasheet\">\n <g>\n <path\n class=\"product-documents__svg-pdf\"\n d=\"M191.92,68.77l-47.69-47.69c-1.33-1.33-3.12-2.08-5.01-2.08H45.09C41.17,19,38,22.17,38,26.09v184.36\n c0,3.92,3.17,7.09,7.09,7.09h141.82c3.92,0,7.09-3.17,7.09-7.09V73.8C194,71.92,193.25,70.1,191.92,68.77z M177.65,77.06h-41.7\n v-41.7L177.65,77.06z M178.05,201.59H53.95V34.95h66.92v47.86c0,5.14,4.17,9.31,9.31,9.31h47.86V201.59z\" />\n </g>\n <rect\n x=\"20\"\n y=\"112\"\n class=\"product-documents__svg-background\"\n width=\"146\"\n height=\"76\" />\n <g>\n <path\n class=\"product-documents__svg-pdf\"\n d=\"M23.83,125.68h22.36c5.29,0,9.41,1.33,12.35,4c2.94,2.67,4.42,6.39,4.42,11.18c0,4.78-1.47,8.51-4.42,11.18\n c-2.94,2.67-7.06,4-12.35,4H34.59v18.29H23.83V125.68z M44.81,147.9c5.38,0,8.07-2.32,8.07-6.97c0-2.39-0.67-4.16-2-5.31\n c-1.33-1.15-3.36-1.73-6.07-1.73H34.59v14.01H44.81z\" />\n <path\n class=\"product-documents__svg-pdf\"\n d=\"M69.92,125.68h18.91c5.29,0,9.84,0.97,13.66,2.9c3.82,1.93,6.74,4.72,8.76,8.35\n c2.02,3.63,3.04,7.98,3.04,13.04c0,5.06-1,9.42-3,13.08c-2,3.66-4.91,6.45-8.73,8.38c-3.82,1.93-8.4,2.9-13.73,2.9H69.92V125.68z\n M88.07,165.63c10.35,0,15.52-5.22,15.52-15.66c0-10.4-5.17-15.59-15.52-15.59h-7.38v31.26H88.07z\" />\n <path\n class=\"product-documents__svg-pdf\"\n d=\"M122.57,125.68h32.84v8.49h-22.22v11.18h20.84v8.49h-20.84v20.49h-10.63V125.68z\" />\n </g>\n </svg>\n <span class=\"product-documents__info\"\n >CUT&Tag Protocol for Histone PTMs</span\n >\n </a>\n </div>\n </div>\n </div>\n </div>\n <div id=\"prodAccordion\">\n <div id=\"ProductDescription\" class=\"Block Panel\">\n <h3 class=\"sub-title1\">Intellectual Property</h3>\n <div\n class=\"ProductDescriptionContainer product-droppdown__section-description\">\n <p>\n US Pat. No. 10,689,643, 11,306,307, EU Pat. No. 3,688,157, 2,999,784\n and related patents and pending applications\n </p>\n </div>\n </div>\n </div>\n </div>\n</div>\n\n<style>\n .button-group {\n display: none;\n }\n /* .product-price-line > span {\n display: none;\n } */\n\n table {\n width: 65%;\n }\n th {\n text-decoration: underline;\n }\n td,\n th {\n text-align: left;\n padding: 8px;\n }\n th,\n td,\n tr {\n color: #000;\n }\n\n /* Tablet */\n\n @media only screen and (max-width: 949px),\n (min-device-width: 468px) and (max-device-width: 949px) {\n /* Force table to not be like tables anymore */\n table,\n thead,\n tbody,\n th,\n td,\n tr {\n display: block;\n }\n th {\n display: none !important;\n }\n\n /* Hide table headers (but not display: none;, for accessibility) */\n thead tr {\n position: absolute;\n top: -9999px;\n left: -9999px;\n }\n\n tr {\n border: 1px solid #ccc;\n margin-bottom: 1rem !important;\n }\n\n td {\n /* Behave like a \"row\" */\n border: none;\n border-bottom: 1px solid lightgray !important;\n position: relative;\n padding-left: 50%;\n display: flex !important;\n justify-content: flex-start !important;\n }\n\n td:before {\n /* Now like a table header */\n /* position: absolute; 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Product Discontinued - See 14-1102 and 14-1103 as recommended alternatives
Description
The CUTANA™ CUT&Tag Core Reagents Bundle brings together the essential components of a CUT&Tag kit, offering a significant cost savings over purchasing each individual reagent. Detailed instructions for using the bundled reagents can be found in the EpiCypher CUT&Tag protocol. Included are ConA magnetic beads for immobilizing nuclei or cells, control antibodies, anti-rabbit secondary antibody, spike-in controls, pAG-Tn5 for antibody-targeted chromatin tagmentation, and PCR master mix for library amplification. The positive and negative control antibodies (H3K4me3, IgG) can be paired with the SNAP-CUTANA™ K-MetStat Panel of spike-in controls for assay optimization and reaction monitoring.
Some necessary reagents including CUT&Tag assay buffers, primers, and DNA purification beads are not included in the bundle (see Required Materials Not Supplied). For detailed buffer recipes, multiplexing primer sequences and recommended vendors, see the CUT&Tag protocol.
The EpiCypher CUT&Tag protocol is compatible with a variety of inputs, though nuclei are preferred to reduce mitochondrial background. While it is recommended to start with 100,000 cells, comparable data can be generated using as few as 1,000 cells. CUT&Tag provides robust results when profiling histone post-translational modifications (PTMs), but is not recommended for chromatin associated proteins (CAPs). The inclusion of controls, low cell tolerance, and robust PTM profiling potential make the CUT&Tag bundle the ideal choice for experienced users looking for high throughput chromatin mapping. For new users, projects mapping CAPs, or projects mapping histone PTMs where >5,000 cells / sample can be attained, check out the CUTANA™ ChIC/CUT&RUN Kit (EpiCypher 14-1048) for an all-inclusive and robust epigenomic profiling solution.
Bundle Contents
Item | Pack Size | CAT |
---|---|---|
pAG-Tn5 | 50 Reactions | 15-1017 |
Concanavalin A Beads | 50 Reactions | 21-1401 |
SNAP-CUTANA™ K-MetStat Panel | 20 Reactions | 19-1002t |
H3K4me3 Control Antibody | 10 Reactions | 13-0041t |
Rabbit IgG Control Antibody | 10 Reactions | 13-0042t |
Anti-rabbit secondary | 50 Reactions | 13-0047 |
High Fidelity 2X PCR Master Mix | 50 Reactions | 15-1018 |
Validation Data
For product-specific validation data, see the individual product pages linked above
Required Materials Not Supplied
The following reagents are not included. See our CUT&Tag protocol for more information:
- Assay buffers
- Multiplexing primers (requires custom oligo synthesis)
- SPRI Beads (e.g. AMPure XP, SPRIselect)
- 8-strip tubes & magnets
- Qubit™ 4 Fluorometer & 1X dsDNA HS Kit
- Agilent BioAnalyzer® or TapeStation®
- If using a mouse primary antibody, the anti-mouse secondary antibody (EpiCypher 13-0048) must be purchased separately.
Technical Information
Additional Information
Beckman Coulter, the stylized logo, AMPure XP, and SPRIselect are trademarks or registered trademarks of Beckman Coulter, Inc. in the United States and other countries.
Documents & Resources
Intellectual Property
US Pat. No. 10,689,643, 11,306,307, EU Pat. No. 3,688,157, 2,999,784 and related patents and pending applications