Anti-Rabbit

Anti-Rabbit Secondary Antibody for CUTANA™ CUT&Tag Workflows

$75.00
SKU: 13-0047
Select Pack Size: *
  • Type:  Mixed Monoclonal*
  • Host:  Goat
  • Applications:  CUT&Tag
  • Reactivity:  Anti-Rabbit
  • Format:  Affinity Purified IgG
  • Target Size:  N/A

Description

Anti-Rabbit Secondary Antibody for CUTANA™ CUT&Tag is affinity purified and specific for rabbit immunoglobulins. Minimal cross reactivity with mouse, rat, human, bovine, guinea pig, and donkey IgG is observed. Use of a secondary antibody in CUT&Tag aids in signal amplification by increasing bound IgG per target epitope. For best results, use with CUTANA pAG-Tn5 (EpiCypher 15-1017 / 15-1117).

*Mixed Monoclonal: a pool of multiple monoclonal antibodies.

Validation Data

Figure 1: CUT&Tag data
CUT&Tag was performed on 100k K562 nuclei with rabbit anti-H3K27me3 antibody (EpiCypher 13-0030) and 0.5 µg of anti-rabbit secondary antibody for CUTANA™ CUT&Tag using the EpiCypher CUTANA™ Direct-to-PCR CUT&Tag protocol. Gene browser shots generated using the Integrative Genomics Viewer (IGV, Broad Institute) show a representative 324 kb window centered at the LAMC3 gene. The genomic distribution pattern was consistent with that of H3K27me3.

Technical Information

Immunogen
Recombinant full-length rabbit immunoglobulin protein.
Formulation
Protein G affinity-purified antibody in PBS pH 7.2, with 0.09% sodium azide.
Storage and Stability
Store at 4°C short term. For long term storage, store at -20°C. Avoid freeze/thaw cycles.

Recommended Dilution

CUT&Tag: 0.5 µg per reaction

Documents & Resources

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