CUTANA™ Concanavalin A Conjugated Paramagnetic Beads Description: This product contains Concanavalin A-conjugated to paramagnetic microspheres. Concanavalin A (ConA) is a lectin (carbohydrate-binding protein) that binds specifically to mannosyl- and glucosyl-containing extracellular glycoproteins. The ConA magnetic beads are therefore useful to immobilize cells or nuclei presenting these glycans in their extracellular matrices.
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CUTANA™ Concanavalin A Conjugated Paramagnetic Beads Formulation: Concanavalin A conjugated to 1 µm paramagnetic microspheres in 10mM PBS with 0.1% sodium azide.
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CUTANA™ Concanavalin A Conjugated Paramagnetic Beads Storage and Stability: Stable for six months at 4°C from date of receipt. DO NOT FREEZE!
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CUTANA™ Concanavalin A Conjugated Paramagnetic Beads Application Notes: For a detailed CUT&RUN protocol using this product, see the EpiCypher CUT&RUN Protocol at: EpiCypher.com/cutana-protocol.
Note: It is recommended to use 10 µL slurry per sample (500,000 cells or less) for ChIC/CUT&RUN. Compatible with the EpiCypher Magnetic Separation Rack (Catalog No. 10-0008).
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Figure 1. CUT&RUN data was generated using EpiCypher CUTANA™ Concanavalin A-Conjugated Paramagnetic Beads (10 µL) with 500,000 K562 cells and H3K4me3 antibody (EpiCypher Catalog No. 13-0041, 1:100 dilution). Shown is a heatmap for H3K4me3 CUT&RUN signal (from 3.9 million paired-end reads) aligned to the transcription start site (TSS, +/- 2kb) of 18,793 genes. High and low signal are ranked by intensity (top to bottom) and reflected by red and blue colors, respectively.
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 | Figure 2. H3K4me3 CUT&RUN data was generated as
described above. Four representative loci are shown in the Integrative Genomics Viewer (IGV, Broad Institute). Window size is denoted at the top of each panel.
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