SKU: 15-1018
{"AddThisServiceButtonMeta":"","add_this":[{"annotation":"","service":"facebook"},{"annotation":"","service":"email"},{"annotation":"","service":"print"},{"annotation":"","service":"twitter"},{"annotation":"","service":"linkedin"}],"add_to_wishlist_url":"/wishlist.php?action=add&product_id=753","availability":"","bulk_discount_rates":[],"can_purchase":true,"cart_url":"https://www.epicypher.com/cart.php","category":["Epigenetics Kits and Reagents/CUTANA™ CUT&Tag Assays"],"custom_fields":[{"id":"1068","name":"Pack Size","value":"50 reactions"},{"id":"1069","name":"Internal Comment","value":"extra in bottom of Venom"}],"customizations":[],"description":"<div class=\"product-general-info\">\n <ul class=\"product-general-info__list-left\">\n <li class=\"product-general-info__list-item\">\n <strong>Type: </strong>Polymerase\n </li>\n <li class=\"product-general-info__list-item\">\n <strong>Mol. Wgt.: </strong>N/A\n </li>\n </ul>\n <ul class=\"product-general-info__list-right\">\n <li class=\"product-general-info__list-item\">\n <strong>Expressed In: </strong><em>E. coli</em>\n </li>\n <li class=\"product-general-info__list-item\">\n <strong>Epitope Tag: </strong>None\n </li>\n </ul>\n</div>\n\n<div class=\"service_accordion product-droppdown\">\n <div class=\"container\">\n <div id=\"prodAccordion\">\n <div id=\"ProductDescription\" class=\"Block Panel current\">\n <h3 class=\"sub-title1\">Description</h3>\n <div\n class=\"ProductDescriptionContainer product-droppdown__section-description-specific\"\n >\n <p>\n CUTANA™ High Fidelity 2X PCR Master Mix for CUT&Tag is formulated\n for non-hot start amplification of next generation sequencing (NGS)\n libraries from CUT&Tag experiments using CUTANA™ pAG-Tn5 (EpiCypher\n <a\n href=\"/products/epigenetics-kits-and-reagents/cutana-pag-tn5-for-cut-and-tag\"\n >15-1017 / 15-1117</a\n >). The master mix requires only the addition of primers and the\n tagmented chromatin to achieve high fidelity amplification of NGS\n libraries.\n </p>\n </div>\n </div>\n </div>\n <div id=\"prodAccordion\">\n <div id=\"ProductDescription\" class=\"Block Panel current\">\n <h3 class=\"sub-title1\">Validation Data</h3>\n <div\n class=\"ProductDescriptionContainer product-droppdown__section-description-specific\"\n >\n <section class=\"image-picker\">\n <div class=\"image-picker__left\">\n <div\n class=\"image-picker__main-content_active image-picker__main-content\"\n >\n <div class=\"image-picker__header-content\">\n <button class=\"image-picker__left-arrow\">\n <svg\n class=\"image-picker__svg-left\"\n width=\"24\"\n height=\"24\"\n viewBox=\"0 0 24 24\"\n >\n <path\n d=\"M16.67 0l2.83 2.829-9.339 9.175 9.339 9.167-2.83 2.829-12.17-11.996z\"\n />\n </svg>\n </button>\n <a\n href=\"/content/images/products/proteins/15-1018-ngs-libraries.jpeg\"\n target=\"_blank\"\n class=\"image-picker__main-image-link\"\n ><img\n alt=\"15-1018-ngs-libraries\"\n src=\"/content/images/products/proteins/15-1018-ngs-libraries.jpeg\"\n class=\"image-picker__main-image\"\n />\n <span class=\"image-picker__main-image-caption\"\n >(Click to enlarge)</span\n ></a\n >\n <button class=\"image-picker__right-arrow\">\n <svg\n class=\"image-picker__svg-right\"\n width=\"24\"\n height=\"24\"\n viewBox=\"0 0 24 24\"\n >\n <path\n d=\"M7.33 24l-2.83-2.829 9.339-9.175-9.339-9.167 2.83-2.829 12.17 11.996z\"\n />\n </svg>\n </button>\n </div>\n <p>\n <span class=\"image-picker__span-content\"\n ><strong\n >Figure 1: CUT&Tag Next-Generation Sequencing (NGS)\n libraries </strong\n ><br />\n CUT&Tag DNA from 100,000 K562 nuclei was directly PCR\n amplified using CUTANA™ High Fidelity 2X PCR Master Mix to\n produce NGS libraries. Tapestation size distribution\n analysis is shown for CUT&Tag libraries using IgG negative\n control (EpiCypher\n <a\n href=\"/products/nucleosomes/snap-cutana-spike-in-controls/cutana-rabbit-igg-cut-run-negative-control-antibody\"\n >13-0042</a\n >) or H3K27me3 (EpiCypher\n <a\n href=\"/products/antibodies/snap-chip-certified-antibodies/histone-h3k27me3-antibody-snap-chip-certified-cutana-cut-run-compatible-discontinued\"\n >13-0030</a\n >) and anti-mouse secondary (EpiCypher\n <a\n href=\"/products/epigenetics-reagents-and-assays/cutanac-cut-tag-assays/anti-mouse-secondary-antibody-for-cutana-cut-tag-workflows\"\n >13-0048</a\n >) antibodies. The High Fidelity Master Mix successfully\n amplified NGS libraries predominantly enriched for\n mononucleosome sized fragments (~300 bp peaks reflect\n nucleosomes + sequence adapters).\n </span>\n </p>\n </div>\n <div class=\"image-picker__main-content\">\n <div class=\"image-picker__header-content\">\n <button class=\"image-picker__left-arrow\">\n <svg\n class=\"image-picker__svg-left\"\n width=\"24\"\n height=\"24\"\n viewBox=\"0 0 24 24\"\n >\n <path\n d=\"M16.67 0l2.83 2.829-9.339 9.175 9.339 9.167-2.83 2.829-12.17-11.996z\"\n />\n </svg>\n </button>\n <a\n href=\"/content/images/products/proteins/15-1018-ngs-data.jpeg\"\n target=\"_blank\"\n class=\"image-picker__main-image-link\"\n ><img\n alt=\"15-1018-ngs-data\"\n src=\"/content/images/products/proteins/15-1018-ngs-data.jpeg\"\n class=\"image-picker__main-image\"\n />\n <span class=\"image-picker__main-image-caption\"\n >(Click to enlarge)</span\n ></a\n >\n <button class=\"image-picker__right-arrow\">\n <svg\n class=\"image-picker__svg-right\"\n width=\"24\"\n height=\"24\"\n viewBox=\"0 0 24 24\"\n >\n <path\n d=\"M7.33 24l-2.83-2.829 9.339-9.175-9.339-9.167 2.83-2.829 12.17 11.996z\"\n />\n </svg>\n </button>\n </div>\n <p>\n <span class=\"image-picker__span-content\"\n ><strong>Figure 2: CUT&Tag NGS data </strong><br />\n CUT&Tag DNA from the CUTANA™ High Fidelity 2X PCR Master Mix\n amplified NGS libraries were subjected to paired-end\n sequencing using an Illumina NextSeq2000. A 240 bp\n representative locus is shown for IgG (EpiCypher\n <a\n href=\"/products/nucleosomes/snap-cutana-spike-in-controls/cutana-rabbit-igg-cut-run-negative-control-antibody\"\n >13-0042</a\n >) or H3K27me3 (EpiCypher\n <a\n href=\"/products/antibodies/snap-chip-certified-antibodies/histone-h3k27me3-antibody-snap-chip-certified-cutana-cut-run-compatible-discontinued\"\n >13-0030</a\n >). NGS libraries amplified using the CUTANA™ 2X PCR Master\n Mix represented the expected genomic distribution of\n H3K27me3. Image was generated using the Integrative Genomics\n Viewer (IGV, Broad Institute).\n </span>\n </p>\n </div>\n </div>\n <aside class=\"image-picker__right\">\n <div class=\"image-picker__gallery\">\n <img\n alt=\"15-1018-ngs-libraries\"\n src=\"/content/images/products/proteins/15-1018-ngs-libraries.jpeg\"\n width=\"200\"\n class=\"image-picker__side-image image-picker__side-image_active\"\n role=\"button\"\n />\n <img\n alt=\"15-1018-ngs-data\"\n src=\"/content/images/products/proteins/15-1018-ngs-data.jpeg\"\n class=\"image-picker__side-image\"\n role=\"button\"\n />\n </div>\n </aside>\n </section>\n </div>\n </div>\n </div>\n <div id=\"prodAccordion\">\n <div id=\"ProductDescription\" class=\"Block Panel\">\n <h3 class=\"sub-title1\">Technical Information</h3>\n <div\n class=\"ProductDescriptionContainer product-droppdown__section-description\"\n >\n <div class=\"product-tech-info\">\n <div class=\"product-tech-info__line-item\">\n <div class=\"product-tech-info__line-item-left\">\n <b>Formulation</b>\n </div>\n <div class=\"product-tech-info__line-item-right\">\n A high fidelity thermostable DNA polymerase with 3'→5'\n exonuclease activity fused to a processivity-enhancing domain in\n a 2x master mix containing 200 µM dNTPs and 4 mM Mg2+ in a\n proprietary buffer.\n </div>\n </div>\n <div class=\"product-tech-info__line-item\">\n <div class=\"product-tech-info__line-item-left\">\n <b>Storage and Stability</b>\n </div>\n <div class=\"product-tech-info__line-item-right\">\n Stable for 6 months at -20°C from date of receipt. The\n master mix should be thawed and resuspended prior to use.\n </div>\n </div>\n </div>\n </div>\n </div>\n </div>\n <div id=\"prodAccordion\">\n <div id=\"ProductDescription\" class=\"Block Panel\">\n <h3 class=\"sub-title1\">Application Notes</h3>\n <div\n class=\"ProductDescriptionContainer product-droppdown__section-description\"\n >\n <p>\n <strong>Recommended use</strong>: After completing pAG-Tn5 chromatin\n tagmentation, add 25 μL of the supplied enzyme into a ~25 μL CUT&Tag\n reaction (2X dilution). For detailed instructions regarding non-hot\n start PCR cycling conditions in CUT&Tag, see CUTANA™ Direct-to-PCR\n CUT&Tag <a href=\"/resources/protocols/\">protocol</a>.\n </p>\n\n <p>\n <strong>NOTE</strong>: Not recommended for use with primers or\n templates containing uracil.\n </p>\n </div>\n </div>\n </div>\n <div id=\"prodAccordion\">\n <div id=\"ProductDescription\" class=\"Block Panel\">\n <h3 class=\"sub-title1\">References</h3>\n <div\n class=\"ProductDescriptionContainer product-droppdown__section-description\"\n >\n <strong>Background References:</strong>\n <br />\n [1] Kaya-Okur et al. <em>Nat. Commun.</em> (2019). PMID:\n <a\n href=\"https://pubmed.ncbi.nlm.nih.gov/31036827/\"\n target=\"_blank\"\n title=\"CUT&Tag for efficient epigenomic profiling of small samples and single cells\"\n >31036827</a\n ><br />\n [2] Henikoff et al. <em>Elife.</em> (2020). PMID:\n <a\n href=\"https://pubmed.ncbi.nlm.nih.gov/33191916/\"\n target=\"_blank\"\n title=\"Efficient chromatin accessibility mapping in situ by nucleosome-tethered tagmentation\"\n >33191916</a\n ><br />\n <br />\n <strong>Product References:</strong>\n <iframe\n class=\"airtable-embed\"\n src=\"https://airtable.com/embed/shrNVh8cMlhS7zB7g?backgroundColor=purple&viewControls=on\"\n frameborder=\"0\"\n onmousewheel=\"\"\n width=\"100%\"\n height=\"300\"\n style=\"background: transparent; border: 1px solid #ccc\"\n ></iframe>\n </div>\n </div>\n </div>\n <div id=\"prodAccordion\">\n <div id=\"ProductDescription\" class=\"Block Panel\">\n <h3 class=\"sub-title1\">Documents & Resources</h3>\n <div\n class=\"ProductDescriptionContainer product-droppdown__section-description\"\n >\n <div class=\"product-documents\">\n <a\n href=\"/content/documents/tds/15-1018.pdf\"\n target=\"_blank\"\n class=\"product-documents__link\"\n >\n <svg\n version=\"1.1\"\n id=\"Layer_1\"\n xmlns=\"http://www.w3.org/2000/svg\"\n xmlns:xlink=\"http://www.w3.org/1999/xlink\"\n x=\"0px\"\n y=\"0px\"\n viewBox=\"0 0 228 240\"\n style=\"enable-background: new 0 0 228 240\"\n xml:space=\"preserve\"\n class=\"product-documents__icon\"\n alt=\"16-0030 Datasheet\"\n >\n <g>\n <path\n class=\"product-documents__svg-pdf\"\n d=\"M191.92,68.77l-47.69-47.69c-1.33-1.33-3.12-2.08-5.01-2.08H45.09C41.17,19,38,22.17,38,26.09v184.36\n c0,3.92,3.17,7.09,7.09,7.09h141.82c3.92,0,7.09-3.17,7.09-7.09V73.8C194,71.92,193.25,70.1,191.92,68.77z M177.65,77.06h-41.7\n v-41.7L177.65,77.06z M178.05,201.59H53.95V34.95h66.92v47.86c0,5.14,4.17,9.31,9.31,9.31h47.86V201.59z\"\n />\n </g>\n <rect\n x=\"20\"\n y=\"112\"\n class=\"product-documents__svg-background\"\n width=\"146\"\n height=\"76\"\n />\n <g>\n <path\n class=\"product-documents__svg-pdf\"\n d=\"M23.83,125.68h22.36c5.29,0,9.41,1.33,12.35,4c2.94,2.67,4.42,6.39,4.42,11.18c0,4.78-1.47,8.51-4.42,11.18\n c-2.94,2.67-7.06,4-12.35,4H34.59v18.29H23.83V125.68z M44.81,147.9c5.38,0,8.07-2.32,8.07-6.97c0-2.39-0.67-4.16-2-5.31\n c-1.33-1.15-3.36-1.73-6.07-1.73H34.59v14.01H44.81z\"\n />\n <path\n class=\"product-documents__svg-pdf\"\n d=\"M69.92,125.68h18.91c5.29,0,9.84,0.97,13.66,2.9c3.82,1.93,6.74,4.72,8.76,8.35\n c2.02,3.63,3.04,7.98,3.04,13.04c0,5.06-1,9.42-3,13.08c-2,3.66-4.91,6.45-8.73,8.38c-3.82,1.93-8.4,2.9-13.73,2.9H69.92V125.68z\n M88.07,165.63c10.35,0,15.52-5.22,15.52-15.66c0-10.4-5.17-15.59-15.52-15.59h-7.38v31.26H88.07z\"\n />\n <path\n class=\"product-documents__svg-pdf\"\n d=\"M122.57,125.68h32.84v8.49h-22.22v11.18h20.84v8.49h-20.84v20.49h-10.63V125.68z\"\n />\n </g>\n </svg>\n <span class=\"product-documents__info\">Technical Datasheet</span>\n </a>\n </div>\n </div>\n </div>\n </div>\n </div>\n</div>\n","detail_messages":"","gift_wrapping_available":false,"gtin":null,"id":"753","images":[{"alt":"CUTANA™ High Fidelity 2X PCR Master 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Tax","without_tax":{"currency":"USD","formatted":"$125.00","value":125}},"rating":0,"related_products":[{"add_to_cart_url":"https://www.epicypher.com/cart.php?action=add&product_id=748","add_to_wishlist_url":"/wishlist.php?action=add&product_id=748","availability":"","brand":{"name":null},"category":["Epigenetics Kits and Reagents","Epigenetics Kits and Reagents/CUTANA™ ChIC / CUT&RUN Assays"],"custom_fields":[{"id":510,"name":"Pack size","value":"100 ng"}],"date_added":"14th May 2020","demo":false,"has_options":false,"id":748,"image":{"alt":"CUTANA™ E. coli Spike-in DNA","data":"https://cdn11.bigcommerce.com/s-y9o92/images/stencil/{:size}/products/748/730/DNA_Vector_Draw_Final-w-border-01__76823.1592491202.png?c=2"},"images":[{"alt":"CUTANA™ E. coli Spike-in DNA","data":"https://cdn11.bigcommerce.com/s-y9o92/images/stencil/{:size}/products/748/730/DNA_Vector_Draw_Final-w-border-01__76823.1592491202.png?c=2"}],"low_stock_level":null,"name":"CUTANA™ E. coli Spike-in DNA","num_reviews":null,"pre_order":false,"price":{"tax_label":"Sales Tax","without_tax":{"currency":"USD","formatted":"$135.00","value":135}},"qty_in_cart":0,"rating":null,"show_cart_action":true,"sku":"18-1401","stock_level":null,"summary":"\n \n \n \n Description\n \n \n Fragmented DNA derived from Escheric","url":"https://www.epicypher.com/products/epigenetics-reagents-and-assays/cutana-e-coli-spike-in-dna","weight":{"formatted":"0.01 LBS","value":0.01}},{"add_to_wishlist_url":"/wishlist.php?action=add&product_id=773","availability":"","brand":{"name":null},"category":["Epigenetics Kits and Reagents","Epigenetics Kits and Reagents/CUTANA™ ChIC / CUT&RUN Assays"],"custom_fields":[{"id":589,"name":"Pack size","value":"50 samples"}],"date_added":"13th Aug 2020","demo":false,"has_options":false,"id":773,"image":{"alt":"CUTANA™ DNA Purification 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2021","demo":false,"has_options":false,"id":804,"image":{"alt":"CHD1 CUTANA™ CUT&RUN Antibody","data":"https://cdn11.bigcommerce.com/s-y9o92/images/stencil/{:size}/products/804/793/2020.12.08_CUTANA_ChAPs_Antibody_thumbnail_RGB_LS__39482.1614276466.png?c=2"},"images":[{"alt":"CHD1 CUTANA™ CUT&RUN Antibody","data":"https://cdn11.bigcommerce.com/s-y9o92/images/stencil/{:size}/products/804/793/2020.12.08_CUTANA_ChAPs_Antibody_thumbnail_RGB_LS__39482.1614276466.png?c=2"}],"low_stock_level":null,"name":"CHD1 CUTANA™ CUT&RUN Antibody","num_reviews":null,"pre_order":false,"price":{"tax_label":"Sales Tax","without_tax":{"currency":"USD","formatted":"$445.00","value":445}},"qty_in_cart":0,"rating":null,"show_cart_action":true,"sku":"13-2008","stock_level":null,"summary":"\n \n \n Type: Polyclonal\n \n \n Host: Rabbit\n \n \n Target Size: 197 kD","url":"https://www.epicypher.com/products/antibodies/chd1-cutana-cut-run-antibody","weight":{"formatted":"0.01 LBS","value":0.01}},{"add_to_cart_url":"https://www.epicypher.com/cart.php?action=add&product_id=906","add_to_wishlist_url":"/wishlist.php?action=add&product_id=906","availability":"","brand":{"name":null},"category":["Antibodies/CUTANA™ CUT&RUN Antibodies","Antibodies/CUTANA™ CUT&RUN Antibodies/CUT&RUN Antibodies - Chromatin-Associated Proteins","Epigenetics Kits and Reagents/CUTANA™ ChIC / CUT&RUN Assays"],"custom_fields":[{"id":1030,"name":"Pack Size","value":"100 µL"}],"date_added":"30th Mar 2022","demo":false,"has_options":false,"id":906,"image":{"alt":"Menin CUTANA™ CUT&RUN Antibody","data":"https://cdn11.bigcommerce.com/s-y9o92/images/stencil/{:size}/products/906/1010/chd3-cutana-cutandrun-antibody__66975.1645734525__91145.1648666822__40012.1648670522.jpg?c=2"},"images":[{"alt":"Menin CUTANA™ CUT&RUN 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Pack Size: 50 reactions
