CUTANA™ Uncharged pAG-Tn5 for CUT&Tag
{"url":"https://www.epicypher.com/products/epigenetics-kits-and-reagents/cutana-cut-tag-assays/cutana-uncharged-pag-tn5-for-cut-tag","add_this":[{"service":"facebook","annotation":""},{"service":"email","annotation":""},{"service":"print","annotation":""},{"service":"twitter","annotation":""},{"service":"linkedin","annotation":""}],"gtin":null,"id":"766","bulk_discount_rates":[],"can_purchase":true,"meta_description":"The key enzyme for performing CUT&Tag, an ultra-sensitive chromatin mapping assay with significant advantages over ChIP-seq.","category":["Epigenetics Kits and Reagents","Epigenetics Kits and Reagents/CUTANA™ CUT&Tag Assays","Other/IDEA Toolbox"],"AddThisServiceButtonMeta":"","main_image":{"data":"https://cdn11.bigcommerce.com/s-y9o92/images/stencil/{:size}/products/766/1065/toolbox-icon__65890.1661116229__53021.1661977262.png?c=2","alt":"CUTANA™ Uncharged pAG-Tn5 for CUT&Tag"},"add_to_wishlist_url":"/wishlist.php?action=add&product_id=766","shipping":{"calculated":true},"num_reviews":0,"weight":"0.01 LBS","custom_fields":[{"id":"1206","name":"Pack Size","value":"20 µL"}],"sku":"15-1025","description":"<div class=\"product-general-info\">\n <ul class=\"product-general-info__list-left\">\n <li class=\"product-general-info__list-item\">\n <strong>Type: </strong>Transposase\n </li>\n <li class=\"product-general-info__list-item\">\n <strong>Mol Wgt: </strong>157 kDa (dimer)\n </li>\n </ul>\n <ul class=\"product-general-info__list-right\">\n <li class=\"product-general-info__list-item\">\n <strong>Host: </strong><em>E. coli</em>\n </li>\n <li class=\"product-general-info__list-item\">\n <strong>Epitope Tag: </strong>None\n </li>\n </ul>\n</div>\n<div class=\"service_accordion product-droppdown\">\n <div class=\"container\">\n <div id=\"prodAccordion\">\n <div id=\"ProductDescription\" class=\"Block Panel current\">\n <h3 class=\"sub-title1\">Description</h3>\n <div\n class=\"ProductDescriptionContainer product-droppdown__section-description-specific\"\n style=\"display: block\">\n <p>\n Products in EpiCypher’s IDEA Toolbox (Innovation and Discovery of\n Epigenetic Applications) offer access to reagents without known or\n fully defined uses, enabling researchers to explore cutting-edge\n applications. Due to their novelty and unexplored potential,\n EpiCypher will engage in limited technical support.\n </p>\n <p>\n CUTANA Uncharged pAG-Tn5 is a fusion of Proteins A and G to\n <em>E. coli</em> transposase (Tn5) and is the key enzyme for CUT&Tag\n [1]. This product is highly purified to remove contaminating\n <em>E. coli</em> DNA. For superior normalization, as well as\n antibody validation and reaction monitoring, SNAP-CUTANA™ nucleosome\n spike-ins (e.g. EpiCypher\n <a href=\"/products/nucleosomes/snap-cutana-k-metstat-panel\"\n >19-1002</a\n >) are recommended.\n <strong\n >This Tn5 is uncharged and must be loaded with user-designed\n mosaic adapter DNA prior to use in CUT&Tag.</strong\n >\n For a charged enzyme that is ready to use, see EpiCypher\n <a\n href=\"/products/epigenetics-kits-and-reagents/cutana-pag-tn5-for-cut-and-tag\"\n >15-1017</a\n >.\n </p>\n </div>\n </div>\n </div>\n <div id=\"prodAccordion\">\n <div id=\"ProductDescription\" class=\"Block Panel current\">\n <h3 class=\"sub-title1\">Validation Data</h3>\n <div\n class=\"ProductDescriptionContainer product-droppdown__section-description-specific\"\n style=\"display: block\">\n <section class=\"image-picker\">\n <div class=\"image-picker__left\">\n <div\n class=\"image-picker__main-content_active image-picker__main-content\">\n <div class=\"image-picker__header-content\">\n <button class=\"image-picker__left-arrow\">\n <svg\n class=\"image-picker__svg-left\"\n width=\"24\"\n height=\"24\"\n viewBox=\"0 0 24 24\">\n <path\n d=\"M16.67 0l2.83 2.829-9.339 9.175 9.339 9.167-2.83 2.829-12.17-11.996z\" />\n </svg>\n </button>\n <a\n href=\"/content/images/products/proteins/15-1025-protein-gel-data.jpeg\"\n target=\"blank\"\n class=\"image-picker__main-image-link\"\n ><img\n loading=\"lazy\"\n alt=\"15-1025-protein-gel-data\"\n src=\"/content/images/products/proteins/15-1025-protein-gel-data.jpeg\"\n class=\"image-picker__main-image\" />\n <span class=\"image-picker__main-image-caption\"\n >(Click to enlarge)</span\n ></a\n >\n <button class=\"image-picker__right-arrow\">\n <svg\n class=\"image-picker__svg-right\"\n width=\"24\"\n height=\"24\"\n viewBox=\"0 0 24 24\">\n <path\n d=\"M7.33 24l-2.83-2.829 9.339-9.175-9.339-9.167 2.83-2.829 12.17 11.996z\" />\n </svg>\n </button>\n </div>\n <p>\n <span class=\"image-picker__span-content\"\n ><strong>Figure 1: Protein gel data </strong><br />\n CUTANA pAG-Tn5, Uncharged (1 µg) was resolved via SDS-PAGE\n and stained with Coomassie blue. The migration and molecular\n weight of the protein standards are indicated.\n </span>\n </p>\n </div>\n </div>\n <aside class=\"image-picker__right\">\n <div class=\"image-picker__gallery\">\n <img\n loading=\"lazy\"\n alt=\"15-1025-protein-gel-data\"\n src=\"/content/images/products/proteins/15-1025-protein-gel-data.jpeg\"\n width=\"200\"\n class=\"image-picker__side-image image-picker__side-image_active\"\n role=\"button\" />\n </div>\n </aside>\n </section>\n </div>\n </div>\n </div>\n <div id=\"prodAccordion\">\n <div id=\"ProductDescription\" class=\"Block Panel\">\n <h3 class=\"sub-title1\">Recommended Accessory Products</h3>\n <div\n class=\"ProductDescriptionContainer product-droppdown__section-description\">\n <table class=\"epicypher-table\">\n <tr>\n <th>Item</th>\n <th>Cat. No.</th>\n </tr>\n\n <tr>\n <td>CUTANA™ Concanavalin A Conjugated Paramagnetic Beads</td>\n <td>\n <a\n href=\"/products/epigenetics-kits-and-reagents/cutana-chic-cut-run-assays/cutana-concanavalin-a-conjugated-paramagnetic-beads\"\n >21-1401</a\n >\n </td>\n </tr>\n\n <tr>\n <td>CUTANA™ Rabbit IgG CUT&RUN Negative Control Antibody</td>\n <td>\n <a\n href=\"/products/nucleosomes/snap-cutana-spike-in-controls/cutana-rabbit-igg-cut-run-negative-control-antibody\"\n >13-0042</a\n >\n </td>\n </tr>\n\n <tr>\n <td>\n Histone H3K4me3 Antibody, SNAP-Certified™ for CUT&RUN and ChIP\n </td>\n <td>\n <a\n href=\"/products/antibodies/snap-chip-certified-antibodies/histone-h3k4me3-antibody-snap-chip-certified-cutana-cut-run-compatible\"\n >13-0041</a\n >\n </td>\n </tr>\n\n <tr>\n <td>Anti-Rabbit Secondary Antibody for CUTANA™ CUT&Tag</td>\n <td>\n <a\n href=\"/products/epigenetics-reagents-and-assays/cutana-cut-tag-assays/anti-rabbit-secondary-antibody-for-cutana-cut-tag-workflows\"\n >13-0047</a\n >\n </td>\n </tr>\n\n <tr>\n <td>Anti-Mouse Secondary Antibody for CUTANA™ CUT&Tag</td>\n <td>\n <a\n href=\"/products/epigenetics-reagents-and-assays/cutanac-cut-tag-assays/anti-mouse-secondary-antibody-for-cutana-cut-tag-workflows\"\n >13-0048</a\n >\n </td>\n </tr>\n\n <tr>\n <td>SNAP-CUTANA™ K-MetStat Panel</td>\n <td>\n <a href=\"/products/nucleosomes/snap-cutana-k-metstat-panel\"\n >19-1002</a\n >\n </td>\n </tr>\n\n <tr>\n <td>CUTANA™ Non-Hot Start 2X PCR Master Mix for CUT&Tag</td>\n <td>\n <a\n href=\"/products/epigenetics-reagents-and-assays/cutana-chic-cut-tag-assays/cutana-high-fidelity-2x-pcr-master-mix\"\n >15-1018</a\n >\n </td>\n </tr>\n\n <tr>\n <td>Magnetic Separation Rack, 0.2 mL Tubes</td>\n <td>\n <a\n href=\"/products/epigenetics-reagents-and-assays/cutana-chic-cut-run-assays/magnetic-separation-rack-0-2-ml-tubes\"\n >10-0008</a\n >\n </td>\n </tr>\n\n <tr>\n <td>Magnetic Separation Rack, 1.5 mL Tubes</td>\n <td>\n <a\n href=\"/products/epigenetics-reagents-and-assays/magnetic-separation-rack-1-5-ml-tubes\"\n >10-0012</a\n >\n </td>\n </tr>\n\n <tr>\n <td>CUTANA™ CUT&RUN 8-strip 0.2 mL Tubes</td>\n <td>\n <a\n href=\"/products/epigenetics-reagents-and-assays/cutana-chic-cut-run-assays/cutana-cut-run-8-strip-0-2-ml-tubes\"\n >10-0009</a\n >\n </td>\n </tr>\n </table>\n </div>\n </div>\n </div>\n <div id=\"prodAccordion\">\n <div id=\"ProductDescription\" class=\"Block Panel\">\n <h3 class=\"sub-title1\">Technical Information</h3>\n <div\n class=\"ProductDescriptionContainer product-droppdown__section-description\">\n <div class=\"product-tech-info\">\n <div class=\"product-tech-info__line-item\">\n <div class=\"product-tech-info__line-item-left\">\n <b>Storage</b>\n </div>\n <div class=\"product-tech-info__line-item-right\">\n Stable for one year at -20°C from date of receipt. The protein\n is not subject to freeze/thaw under these conditions.\n </div>\n </div>\n <div class=\"product-tech-info__line-item\">\n <div class=\"product-tech-info__line-item-left\">\n <b>Formulation</b>\n </div>\n <div class=\"product-tech-info__line-item-right\">\n 50 mM HEPES-KOH pH 7.2, 100 mM NaCl, 0.1 mM EDTA, 1 mM DTT, 0.1%\n Triton X-100, 50% glycerol\n </div>\n </div>\n </div>\n </div>\n </div>\n </div>\n <div id=\"prodAccordion\">\n <div id=\"ProductDescription\" class=\"Block Panel\">\n <h3 class=\"sub-title1\">Application Notes</h3>\n <div\n class=\"ProductDescriptionContainer product-droppdown__section-description\">\n <p>\n pAG-Tn5 transposomes can be assembled as previously described [1].\n In brief, 3.2 µL of a 100 µM equimolar mixture of pre-annealed\n user-defined Adapter-A and user-defined Adapter-B oligonucleotides\n should be mixed with 20 µL of 5.5 µM pAG-Tn5 fusion protein dimer.\n The mixture is then incubated on a rotating platform for 1 hour at\n room temperature and stored at -20°C. Specific activity definition\n of the charged pAG-Tn5 is highly recommended before use in CUT&Tag.\n <strong\n >Due to the confounding variable of user-supplied mosaic adapters,\n EpiCypher will not engage in protocol troubleshooting for this\n reagent</strong\n >. For a pre-charged enzyme that is eligible for technical support,\n see EpiCypher\n <a\n href=\"/products/epigenetics-kits-and-reagents/cutana-pag-tn5-for-cut-and-tag\"\n >15-1017</a\n >.\n </p>\n </div>\n </div>\n </div>\n <div id=\"prodAccordion\">\n <div id=\"ProductDescription\" class=\"Block Panel\">\n <h3 class=\"sub-title1\">References</h3>\n <div\n class=\"ProductDescriptionContainer product-droppdown__section-description\">\n <b>Background References:</b><br />\n [1] Kaya-Okur et al. <em>Nat. Commun.</em> (2019). PMID:\n <a\n href=\"https://pubmed.ncbi.nlm.nih.gov/31036827/\"\n title=\"CUT&Tag for efficient epigenomic profiling of small samples and single cells\"\n target=\"_blank\"\n >31036827</a\n ><br />\n </div>\n </div>\n </div>\n <div id=\"prodAccordion\">\n <div id=\"ProductDescription\" class=\"Block Panel\">\n <h3 class=\"sub-title1\">Documents & Resources</h3>\n <div\n class=\"ProductDescriptionContainer product-droppdown__section-description\">\n <div class=\"product-documents\">\n <a\n href=\"/content/documents/tds/15-1025.pdf\"\n target=\"blank\"\n class=\"product-documents__link\">\n <svg\n version=\"1.1\"\n id=\"Layer_1\"\n xmlns=\"http://www.w3.org/2000/svg\"\n xmlns:xlink=\"http://www.w3.org/1999/xlink\"\n x=\"0px\"\n y=\"0px\"\n viewBox=\"0 0 228 240\"\n style=\"enable-background: new 0 0 228 240\"\n xml:space=\"preserve\"\n class=\"product-documents__icon\">\n <g>\n <path\n class=\"product-documents__svg-pdf\"\n d=\"M191.92,68.77l-47.69-47.69c-1.33-1.33-3.12-2.08-5.01-2.08H45.09C41.17,19,38,22.17,38,26.09v184.36\n c0,3.92,3.17,7.09,7.09,7.09h141.82c3.92,0,7.09-3.17,7.09-7.09V73.8C194,71.92,193.25,70.1,191.92,68.77z M177.65,77.06h-41.7\n v-41.7L177.65,77.06z M178.05,201.59H53.95V34.95h66.92v47.86c0,5.14,4.17,9.31,9.31,9.31h47.86V201.59z\" />\n </g>\n <rect\n x=\"20\"\n y=\"112\"\n class=\"product-documents__svg-background\"\n width=\"146\"\n height=\"76\" />\n <g>\n <path\n class=\"product-documents__svg-pdf\"\n d=\"M23.83,125.68h22.36c5.29,0,9.41,1.33,12.35,4c2.94,2.67,4.42,6.39,4.42,11.18c0,4.78-1.47,8.51-4.42,11.18\n c-2.94,2.67-7.06,4-12.35,4H34.59v18.29H23.83V125.68z M44.81,147.9c5.38,0,8.07-2.32,8.07-6.97c0-2.39-0.67-4.16-2-5.31\n c-1.33-1.15-3.36-1.73-6.07-1.73H34.59v14.01H44.81z\" />\n <path\n class=\"product-documents__svg-pdf\"\n d=\"M69.92,125.68h18.91c5.29,0,9.84,0.97,13.66,2.9c3.82,1.93,6.74,4.72,8.76,8.35\n c2.02,3.63,3.04,7.98,3.04,13.04c0,5.06-1,9.42-3,13.08c-2,3.66-4.91,6.45-8.73,8.38c-3.82,1.93-8.4,2.9-13.73,2.9H69.92V125.68z\n M88.07,165.63c10.35,0,15.52-5.22,15.52-15.66c0-10.4-5.17-15.59-15.52-15.59h-7.38v31.26H88.07z\" />\n <path\n class=\"product-documents__svg-pdf\"\n d=\"M122.57,125.68h32.84v8.49h-22.22v11.18h20.84v8.49h-20.84v20.49h-10.63V125.68z\" />\n </g>\n </svg>\n <span class=\"product-documents__info\">Technical Datasheet</span>\n </a>\n </div>\n </div>\n </div>\n </div>\n <div id=\"prodAccordion\">\n <div id=\"ProductDescription\" class=\"Block Panel\">\n <h3 class=\"sub-title1\">Intellectual Property</h3>\n <div\n class=\"ProductDescriptionContainer product-droppdown__section-description\">\n <p>\n US Pat. No. 10,689,643, 11,306,307, EU Pat. No. 3,688,157, 2,999,784\n and related patents and pending applications\n </p>\n </div>\n </div>\n </div>\n </div>\n</div>\n\n<style>\n .form-field-title .required-text {\n display: none !important;\n }\n\n .form-label {\n padding-left: 2rem;\n }\n\n .form-label-text {\n margin: 0;\n margin-left: 0 !important;\n }\n\n /* //////////// */\n\n /* BIOZ */\n td table {\n margin: 0;\n padding: 6px !important;\n }\n\n span.bioz-w-add-underline {\n font-size: 15px;\n }\n\n /* /////////// */\n\n .form-field-title .required-text {\n display: none !important;\n }\n .form-label {\n padding-left: 2rem;\n }\n .form-label-text {\n margin: 0;\n margin-left: 0 !important;\n }\n\n .bioz-w-header td {\n padding: 0;\n }\n\n .epicypher-table table {\n margin-right: auto;\n margin-left: auto;\n width: 100%;\n border-top: 1px solid lightgray;\n }\n\n .epicypher-table th {\n color: #3b3a48;\n }\n .epicypher-table td {\n color: #3b3a48;\n font-size: 0.9rem;\n }\n .epicypher-table td a {\n text-decoration: underline;\n }\n .epicypher-table td,\n th {\n border: 0.5px solid lightgray;\n text-align: left;\n padding: 8px;\n /* white-space: nowrap; */\n }\n .epicypher-table tr:nth-child(even) {\n background-color: #f9f9f9;\n }\n .epicypher-table td:first-child {\n border-left: 1px solid lightgray;\n }\n /* tr:hover {\n background-color: #ffff99;\n } */\n\n /* Tablet */\n\n @media only screen and (max-width: 760px),\n (min-device-width: 760px) and (max-device-width: 1024px) {\n /* Force table to not be like tables anymore */\n .epicypher-table table,\n thead,\n tbody,\n th,\n td,\n tr {\n display: block;\n }\n .epicypher-table th {\n display: none !important;\n }\n\n /* BIOZ */\n td.bioz-w-tooltipx::before {\n display: none;\n }\n\n /* .epicypher-table table,\n thead,\n tbody,\n th,\n td,\n tr {\n display: inline-table !important;\n } */\n\n /* Hide table headers (but not display: none;, for accessibility) */\n .epicypher-table thead tr {\n position: absolute;\n top: -9999px;\n left: -9999px;\n }\n\n .epicypher-table tr {\n border: 1px solid #ccc;\n }\n\n .epicypher-table td {\n /* Behave like a \"row\" */\n /* border: none; */\n border-bottom: 1px solid #eee;\n position: relative;\n padding-left: 30%;\n white-space: inherit;\n }\n\n .epicypher-table td:before {\n /* Now like a table header */\n position: absolute;\n /* Top/left values mimic padding */\n top: 6px;\n left: 6px;\n width: 45%;\n padding-right: 10px;\n white-space: nowrap;\n font-weight: 700;\n }\n\n /*\n Label the data\n */\n .epicypher-table td:nth-of-type(1):before {\n content: 'Item';\n }\n td:nth-of-type(2):before {\n content: 'Cat. No.';\n }\n }\n\n /* @media screen and (max-width: 767px) {\n .custom-title-description {\n font-size: 19px !important;\n }\n .section-title {\n font-size: 19px !important;\n padding-left: 1rem;\n }\n } */\n\n @media screen and (min-width: 768px) {\n .products-featured .container,\n .products-featured .product-tabs,\n .products-related .container,\n .products-related .product-tabs {\n padding-left: 30px !important;\n padding-right: 50px;\n max-width: 1170px !important;\n }\n }\n\n @media screen and (max-width: 767px) {\n .custom-title-description {\n font-size: 19px !important;\n }\n\n .section-title {\n font-size: 19px !important;\n padding-left: 1rem;\n }\n }\n</style>\n","tags":[],"warranty":"","price":{"without_tax":{"formatted":"$645.00","value":645,"currency":"USD"},"tax_label":"Sales Tax"},"detail_messages":"","availability":"","page_title":"CUTANA™ Uncharged pAG-Tn5 for CUT&Tag","cart_url":"https://www.epicypher.com/cart.php","max_purchase_quantity":0,"mpn":null,"upc":null,"options":[],"related_products":[{"id":1160,"sku":"15-1028","name":"CUTANA™ Uncharged 6xHis-pAG-Tn5 for CUT&Tag","url":"https://www.epicypher.com/products/epigenetics-kits-and-reagents/cutana-cut-tag-assays/cutana-uncharged-6xhis-pag-tn5-for-cut-tag","availability":"","rating":null,"brand":{"name":null},"category":["Epigenetics Kits and Reagents","Epigenetics Kits and Reagents/CUTANA™ CUT&Tag Assays","Other/IDEA Toolbox"],"summary":"\n \n \n Type: Transposase\n \n \n Mol Wgt: 161,401.8 Da (dimer)\n \n \n \n ","image":{"data":"https://cdn11.bigcommerce.com/s-y9o92/images/stencil/{:size}/products/1160/1233/toolbox-icon__65890.1661116229__13690.1718896281.png?c=2","alt":"CUTANA™ Uncharged 6xHis-pAG-Tn5 for CUT&Tag"},"images":[{"data":"https://cdn11.bigcommerce.com/s-y9o92/images/stencil/{:size}/products/1160/1233/toolbox-icon__65890.1661116229__13690.1718896281.png?c=2","alt":"CUTANA™ Uncharged 6xHis-pAG-Tn5 for CUT&Tag"}],"date_added":"20th Jun 2024","pre_order":false,"show_cart_action":true,"has_options":false,"stock_level":null,"low_stock_level":null,"qty_in_cart":0,"custom_fields":[{"id":1326,"name":"Pack Size","value":"75 µL"}],"num_reviews":null,"weight":{"formatted":"0.01 LBS","value":0.01},"demo":false,"add_to_cart_url":"https://www.epicypher.com/cart.php?action=add&product_id=1160","price":{"without_tax":{"currency":"USD","formatted":"$645.00","value":645},"tax_label":"Sales Tax"},"add_to_wishlist_url":"/wishlist.php?action=add&product_id=1160"},{"id":764,"sku":null,"name":"CUTANA™ pAG-Tn5 for CUT&Tag","url":"https://www.epicypher.com/products/epigenetics-kits-and-reagents/cutana-pag-tn5-for-cut-and-tag","availability":"","rating":null,"brand":{"name":null},"category":["Epigenetics Kits and Reagents","Epigenetics Kits and Reagents/CUTANA™ CUT&Tag Assays"],"summary":"\n \n \n Type: Transposase\n \n \n Mol Wgt: 191 kDa\n \n \n \n ","image":{"data":"https://cdn11.bigcommerce.com/s-y9o92/images/stencil/{:size}/products/764/750/2020_CUTTag_icon_RGB_Andy_L__93238.1592491196.png?c=2","alt":"CUTANA™ pAG-Tn5 for CUT&Tag"},"images":[{"data":"https://cdn11.bigcommerce.com/s-y9o92/images/stencil/{:size}/products/764/750/2020_CUTTag_icon_RGB_Andy_L__93238.1592491196.png?c=2","alt":"CUTANA™ pAG-Tn5 for CUT&Tag"}],"date_added":"17th Jun 2020","pre_order":false,"show_cart_action":true,"has_options":true,"stock_level":null,"low_stock_level":null,"qty_in_cart":0,"custom_fields":[{"id":1005,"name":"Internal Comment","value":"extra boxes in bottom shelf of Venom"}],"num_reviews":null,"weight":{"formatted":"0.01 LBS","value":0.01},"demo":false,"price":{"without_tax":{"currency":"USD","formatted":"$795.00","value":795},"price_range":{"min":{"without_tax":{"currency":"USD","formatted":"$795.00","value":795},"tax_label":"Sales Tax"},"max":{"without_tax":{"currency":"USD","formatted":"$2,995.00","value":2995},"tax_label":"Sales Tax"}},"tax_label":"Sales Tax"},"add_to_wishlist_url":"/wishlist.php?action=add&product_id=764"},{"id":934,"sku":"15-1026","name":"CUTANA™ Fluorescent pAG-Tn5 for CUT&Tag","url":"https://www.epicypher.com/products/epigenetics-kits-and-reagents/cutana-cut-tag-assays/cutana-fluorescent-pag-tn5-for-cut-tag","availability":"","rating":null,"brand":{"name":null},"category":["Epigenetics Kits and Reagents","Epigenetics Kits and Reagents/CUTANA™ CUT&Tag Assays","Other/IDEA Toolbox"],"summary":"\n \n \n Type: Transposase\n \n \n Mol Wgt: 192 kDa\n \n \n \n ","image":{"data":"https://cdn11.bigcommerce.com/s-y9o92/images/stencil/{:size}/products/934/1048/toolbox-icon__95574.1661116173.png?c=2","alt":"CUTANA™ Fluorescent pAG-Tn5 for CUT&Tag"},"images":[{"data":"https://cdn11.bigcommerce.com/s-y9o92/images/stencil/{:size}/products/934/1048/toolbox-icon__95574.1661116173.png?c=2","alt":"CUTANA™ Fluorescent pAG-Tn5 for CUT&Tag"}],"date_added":"12th Aug 2022","pre_order":false,"show_cart_action":true,"has_options":false,"stock_level":null,"low_stock_level":null,"qty_in_cart":0,"custom_fields":[{"id":1152,"name":"Pack Size","value":"125 µL"},{"id":1153,"name":"Internal Comment","value":"extra boxes in bottom shelf of Venom"}],"num_reviews":null,"weight":{"formatted":"0.01 LBS","value":0.01},"demo":false,"add_to_cart_url":"https://www.epicypher.com/cart.php?action=add&product_id=934","price":{"without_tax":{"currency":"USD","formatted":"$745.00","value":745},"tax_label":"Sales Tax"},"add_to_wishlist_url":"/wishlist.php?action=add&product_id=934"},{"id":986,"sku":null,"name":"CUTANA™ CUT&Tag Kit","url":"https://www.epicypher.com/products/epigenetics-kits-and-reagents/cutana-cut-and-tag-kit","availability":"","rating":null,"brand":{"name":null},"category":["Antibodies/CUTANA™ CUT&Tag Antibodies","Epigenetics Kits and Reagents/CUTANA™ CUT&Tag Assays"],"summary":"\n \n \n \n \n ","image":{"data":"https://cdn11.bigcommerce.com/s-y9o92/images/stencil/{:size}/products/986/1213/CTK20__46720.1713641110.png?c=2","alt":"CUTANA™ CUT&Tag Kit"},"images":[{"data":"https://cdn11.bigcommerce.com/s-y9o92/images/stencil/{:size}/products/986/1213/CTK20__46720.1713641110.png?c=2","alt":"CUTANA™ CUT&Tag Kit"}],"date_added":"8th Mar 2023","pre_order":false,"show_cart_action":true,"has_options":true,"stock_level":null,"low_stock_level":null,"qty_in_cart":0,"custom_fields":[{"id":1219,"name":"Pack Size","value":"48 Reactions"}],"num_reviews":null,"weight":{"formatted":"0.01 LBS","value":0.01},"demo":false,"price":{"without_tax":{"currency":"USD","formatted":"$2,695.00","value":2695},"tax_label":"Sales Tax"},"add_to_wishlist_url":"/wishlist.php?action=add&product_id=986"},{"id":694,"sku":null,"name":"CUTANA™ pAG-MNase for ChIC/CUT&RUN Workflows","url":"https://www.epicypher.com/products/epigenetics-reagents-and-assays/cutana-pag-mnase-for-chic-cut-and-run-workflows","availability":"","rating":null,"brand":{"name":null},"category":["Epigenetics Kits and Reagents","Epigenetics Kits and Reagents/CUTANA™ ChIC / CUT&RUN Assays"],"summary":"\n \n \n Type: Nuclease\n \n \n Mol Wgt: 43.7 kDa\n \n \n \n ","image":{"data":"https://cdn11.bigcommerce.com/s-y9o92/images/stencil/{:size}/products/694/689/Screen_Shot_2020-02-12_at_11.01.55_AM__17144.1581530752.png?c=2","alt":"CUTANA™ pAG-MNase for ChIC/CUT&RUN Workflows"},"images":[{"data":"https://cdn11.bigcommerce.com/s-y9o92/images/stencil/{:size}/products/694/689/Screen_Shot_2020-02-12_at_11.01.55_AM__17144.1581530752.png?c=2","alt":"CUTANA™ pAG-MNase for ChIC/CUT&RUN Workflows"}],"date_added":"12th Aug 2019","pre_order":false,"show_cart_action":true,"has_options":true,"stock_level":null,"low_stock_level":null,"qty_in_cart":0,"custom_fields":[{"id":1174,"name":"Internal Comment","value":"Excess in bottom of Venom"},{"id":1175,"name":"Internal Comment","value":"Bulk in Psylocke"}],"num_reviews":null,"weight":{"formatted":"0.01 LBS","value":0.01},"demo":false,"price":{"without_tax":{"currency":"USD","formatted":"$335.00","value":335},"price_range":{"min":{"without_tax":{"currency":"USD","formatted":"$335.00","value":335},"tax_label":"Sales Tax"},"max":{"without_tax":{"currency":"USD","formatted":"$1,295.00","value":1295},"tax_label":"Sales Tax"}},"tax_label":"Sales Tax"},"add_to_wishlist_url":"/wishlist.php?action=add&product_id=694"},{"id":875,"sku":"13-2010","name":"HA Tag CUTANA™ CUT&RUN Antibody","url":"https://www.epicypher.com/products/antibodies/cutana-cut-run-compatible-antibodies/ha-tag-cutana-cut-and-run-antibody","availability":"","rating":null,"brand":{"name":null},"category":["Antibodies/CUTANA™ CUT&RUN Antibodies","Antibodies/CUTANA™ CUT&RUN Antibodies/CUT&RUN Antibodies - Chromatin-Associated Proteins","Epigenetics Kits and Reagents/CUTANA™ ChIC / CUT&RUN Assays"],"summary":"\n \n \n Type: Polyclonal\n \n \n Host: Rabbit\n \n \n Applications: CUT&RUN, WB","image":{"data":"https://cdn11.bigcommerce.com/s-y9o92/images/stencil/{:size}/products/875/937/ha-tag-cutana-cutandrun-antibody__43540.1645734529.jpg?c=2","alt":"HA Tag CUTANA CUTandRUN Antibody"},"images":[{"data":"https://cdn11.bigcommerce.com/s-y9o92/images/stencil/{:size}/products/875/937/ha-tag-cutana-cutandrun-antibody__43540.1645734529.jpg?c=2","alt":"HA Tag CUTANA CUTandRUN Antibody"}],"date_added":"21st Jul 2021","pre_order":false,"show_cart_action":true,"has_options":false,"stock_level":null,"low_stock_level":null,"qty_in_cart":0,"custom_fields":[{"id":909,"name":"Pack Size","value":"100 µg"}],"num_reviews":null,"weight":{"formatted":"0.00 LBS","value":0},"demo":false,"add_to_cart_url":"https://www.epicypher.com/cart.php?action=add&product_id=875","price":{"without_tax":{"currency":"USD","formatted":"$495.00","value":495},"tax_label":"Sales Tax"},"add_to_wishlist_url":"/wishlist.php?action=add&product_id=875"},{"id":1136,"sku":"13-2031","name":"DYKDDDDK Tag CUTANA™ CUT&RUN Antibody","url":"https://www.epicypher.com/products/epigenetics-kits-and-reagents/cutana-chic-cut-run-assays/dykddddk-tag-cutana-cut-and-run-antibody","availability":"","rating":null,"brand":{"name":null},"category":["Antibodies/CUTANA™ CUT&RUN Antibodies","Antibodies/CUTANA™ CUT&RUN Antibodies/CUT&RUN Antibodies - Chromatin-Associated Proteins","Epigenetics Kits and Reagents/CUTANA™ ChIC / CUT&RUN Assays"],"summary":"\n \n \n Type: Monoclonal\n \n \n Host: Rabbit\n \n \n Applications: CUT&RUN, EL","image":{"data":"https://cdn11.bigcommerce.com/s-y9o92/images/stencil/{:size}/products/1136/1207/ha-tag-cutana-cutandrun-antibody__18433.1706257992.jpg?c=2","alt":"HA Tag CUTANA CUTandRUN Antibody"},"images":[{"data":"https://cdn11.bigcommerce.com/s-y9o92/images/stencil/{:size}/products/1136/1207/ha-tag-cutana-cutandrun-antibody__18433.1706257992.jpg?c=2","alt":"HA Tag CUTANA CUTandRUN Antibody"}],"date_added":"26th Jan 2024","pre_order":false,"show_cart_action":true,"has_options":false,"stock_level":null,"low_stock_level":null,"qty_in_cart":0,"custom_fields":[{"id":1301,"name":"Pack Size","value":"100 µL"}],"num_reviews":null,"weight":{"formatted":"0.00 LBS","value":0},"demo":false,"add_to_cart_url":"https://www.epicypher.com/cart.php?action=add&product_id=1136","price":{"without_tax":{"currency":"USD","formatted":"$495.00","value":495},"tax_label":"Sales Tax"},"add_to_wishlist_url":"/wishlist.php?action=add&product_id=1136"},{"id":890,"sku":"14-1101","name":"CUTANA™ CUT&Tag Core Reagents Bundle *DISCONTINUED*","url":"https://www.epicypher.com/products/epigenetics-kits-and-reagents/cutana-cut-tag-core-reagents-bundle","availability":"","rating":null,"brand":{"name":null},"category":[],"summary":"\n \n Product Discontinued - See 14-1102 and 14-1103 as recommended alternatives\n \n\n","image":{"data":"https://cdn11.bigcommerce.com/s-y9o92/images/stencil/{:size}/products/890/918/2020_CUTTag_icon_RGB_Andy_L__93238.1592491196__35422.1643742241.png?c=2","alt":"CUTANA™ CUT&Tag Core Reagents Bundle *DISCONTINUED*"},"images":[{"data":"https://cdn11.bigcommerce.com/s-y9o92/images/stencil/{:size}/products/890/918/2020_CUTTag_icon_RGB_Andy_L__93238.1592491196__35422.1643742241.png?c=2","alt":"CUTANA™ CUT&Tag Core Reagents Bundle *DISCONTINUED*"}],"date_added":"31st Jan 2022","pre_order":false,"show_cart_action":false,"has_options":false,"stock_level":null,"low_stock_level":null,"qty_in_cart":0,"custom_fields":[{"id":984,"name":"Pack Size","value":"50 Reactions"}],"num_reviews":null,"weight":{"formatted":"0.01 LBS","value":0.01},"demo":false,"price":{"without_tax":{"currency":"USD","formatted":"$1,295.00","value":1295},"tax_label":"Sales Tax"},"add_to_wishlist_url":"/wishlist.php?action=add&product_id=890"},{"id":771,"sku":null,"name":"Anti-Mouse Secondary Antibody for CUTANA™ CUT&Tag Workflows ","url":"https://www.epicypher.com/products/epigenetics-reagents-and-assays/cutanac-cut-tag-assays/anti-mouse-secondary-antibody-for-cutana-cut-tag-workflows","availability":"","rating":null,"brand":{"name":null},"category":["CUTANA™ CUT&Tag Antibodies","Antibodies/CUTANA™ CUT&Tag Antibodies","Epigenetics Kits and Reagents/CUTANA™ CUT&Tag Assays"],"summary":"\n \n \n Type: Mixed Monoclonal*\n \n \n Host: Goat\n \n \n Applications: ","image":{"data":"https://cdn11.bigcommerce.com/s-y9o92/images/stencil/{:size}/products/771/943/anti-mouse-secondary-antibody-for-cutana-cutandtag-workflows__06671.1645734534.jpg?c=2","alt":"Anti-Mouse Secondary Antibody for CUTANA CUTandTag Workflows"},"images":[{"data":"https://cdn11.bigcommerce.com/s-y9o92/images/stencil/{:size}/products/771/943/anti-mouse-secondary-antibody-for-cutana-cutandtag-workflows__06671.1645734534.jpg?c=2","alt":"Anti-Mouse Secondary Antibody for CUTANA CUTandTag Workflows"}],"date_added":"23rd Jul 2020","pre_order":false,"show_cart_action":true,"has_options":true,"stock_level":null,"low_stock_level":null,"qty_in_cart":0,"custom_fields":[{"id":986,"name":"Internal Comment","value":"extra located top-right of Venom"}],"num_reviews":null,"weight":{"formatted":"0.01 LBS","value":0.01},"demo":false,"price":{"without_tax":{"currency":"USD","formatted":"$105.00","value":105},"price_range":{"min":{"without_tax":{"currency":"USD","formatted":"$105.00","value":105},"tax_label":"Sales Tax"},"max":{"without_tax":{"currency":"USD","formatted":"$315.00","value":315},"tax_label":"Sales Tax"}},"tax_label":"Sales Tax"},"add_to_wishlist_url":"/wishlist.php?action=add&product_id=771"}],"shipping_messages":[],"rating":0,"meta_keywords":"CUT&Tag, pAG-Tn5, Tn5, transposase, tagmentation, cutandtag, chromatin mapping, epigenomic mapping, ChIP-seq, Cleavage Under Targets and Tagmentation","show_quantity_input":1,"title":"CUTANA™ Uncharged pAG-Tn5 for CUT&Tag","gift_wrapping_available":false,"min_purchase_quantity":0,"customizations":[],"images":[{"data":"https://cdn11.bigcommerce.com/s-y9o92/images/stencil/{:size}/products/766/1065/toolbox-icon__65890.1661116229__53021.1661977262.png?c=2","alt":"CUTANA™ Uncharged pAG-Tn5 for CUT&Tag"}]} Pack Size: 20 µL
- Type: Transposase
- Mol Wgt: 157 kDa (dimer)
- Host: E. coli
- Epitope Tag: None
Description
Products in EpiCypher’s IDEA Toolbox (Innovation and Discovery of Epigenetic Applications) offer access to reagents without known or fully defined uses, enabling researchers to explore cutting-edge applications. Due to their novelty and unexplored potential, EpiCypher will engage in limited technical support.
CUTANA Uncharged pAG-Tn5 is a fusion of Proteins A and G to E. coli transposase (Tn5) and is the key enzyme for CUT&Tag [1]. This product is highly purified to remove contaminating E. coli DNA. For superior normalization, as well as antibody validation and reaction monitoring, SNAP-CUTANA™ nucleosome spike-ins (e.g. EpiCypher 19-1002) are recommended. This Tn5 is uncharged and must be loaded with user-designed mosaic adapter DNA prior to use in CUT&Tag. For a charged enzyme that is ready to use, see EpiCypher 15-1017.
Recommended Accessory Products
Item | Cat. No. |
---|---|
CUTANA™ Concanavalin A Conjugated Paramagnetic Beads | 21-1401 |
CUTANA™ Rabbit IgG CUT&RUN Negative Control Antibody | 13-0042 |
Histone H3K4me3 Antibody, SNAP-Certified™ for CUT&RUN and ChIP | 13-0041 |
Anti-Rabbit Secondary Antibody for CUTANA™ CUT&Tag | 13-0047 |
Anti-Mouse Secondary Antibody for CUTANA™ CUT&Tag | 13-0048 |
SNAP-CUTANA™ K-MetStat Panel | 19-1002 |
CUTANA™ Non-Hot Start 2X PCR Master Mix for CUT&Tag | 15-1018 |
Magnetic Separation Rack, 0.2 mL Tubes | 10-0008 |
Magnetic Separation Rack, 1.5 mL Tubes | 10-0012 |
CUTANA™ CUT&RUN 8-strip 0.2 mL Tubes | 10-0009 |
Technical Information
Application Notes
pAG-Tn5 transposomes can be assembled as previously described [1]. In brief, 3.2 µL of a 100 µM equimolar mixture of pre-annealed user-defined Adapter-A and user-defined Adapter-B oligonucleotides should be mixed with 20 µL of 5.5 µM pAG-Tn5 fusion protein dimer. The mixture is then incubated on a rotating platform for 1 hour at room temperature and stored at -20°C. Specific activity definition of the charged pAG-Tn5 is highly recommended before use in CUT&Tag. Due to the confounding variable of user-supplied mosaic adapters, EpiCypher will not engage in protocol troubleshooting for this reagent. For a pre-charged enzyme that is eligible for technical support, see EpiCypher 15-1017.
References
Documents & Resources
Intellectual Property
US Pat. No. 10,689,643, 11,306,307, EU Pat. No. 3,688,157, 2,999,784 and related patents and pending applications