
ACF Chromatin Remodeling Enzyme Complex
$505.00
SKU: 15-1013
{"AddThisServiceButtonMeta":"","add_this":[{"annotation":"","service":"facebook"},{"annotation":"","service":"email"},{"annotation":"","service":"print"},{"annotation":"","service":"twitter"},{"annotation":"","service":"linkedin"}],"add_to_wishlist_url":"/wishlist.php?action=add&product_id=551","availability":"","bulk_discount_rates":[],"can_purchase":true,"cart_url":"https://www.epicypher.com/cart.php","category":["Nucleosomes/EpiDyne® Chromatin Remodeling Substrates/EpiDyne<sup>®</sup> Modifying Enzymes ","Recombinant Proteins/EpiDyne<sup>®</sup> Chromatin Remodeling Enzymes","Recombinant Proteins/Modifying Enzymes"],"custom_fields":[{"id":"585","name":"Pack Size","value":"100 reactions"}],"customizations":[],"description":"<table width=\"100%\" border=\"0\" cellspacing=\"2\" cellpadding=\"2\">\n \n <tbody>\n \n <tr valign=\"top\">\n \n <td>\n \n \n \n <table width=\"95%\" border=\"0\" cellspacing=\"1\" cellpadding=\"1\">\n \n <tbody>\n \n <tr>\n \n <td>\n \n <table width=\"100%\" border=\"0\" cellspacing=\"0\" cellpadding=\"0\">\n \n <tbody>\n \n <tr>\n \n <td align=\"left\" valign=\"top\"><span style=\"font-family: Arial; \" class=\"Apple-style-span\"><span style=\"font-size: 10pt; \" class=\"Apple-style-span\"><b>Type: </b>Remodeler/ATPase</span></span>\n \n </td>\n \n <td width=\"196\" align=\"left\" valign=\"top\"><span style=\"font-family: Arial; \" class=\"Apple-style-span\"><span style=\"font-size: 10pt; \" class=\"Apple-style-span\"><b>Host: </b>SF9 cells</span></span>\n \n </td>\n \n </tr>\n \n <tr>\n \n <td align=\"left\" valign=\"top\"><span style=\"font-family: Arial; \" class=\"Apple-style-span\"><span style=\"font-size: 10pt; \" class=\"Apple-style-span\"><b>Mol Wgt.: </b>185 kDa</span></span>\n \n </td>\n \n <td align=\"left\" valign=\"top\"><span style=\"font-family: Arial; \" class=\"Apple-style-span\"><span style=\"font-size: 10pt; \" class=\"Apple-style-span\"><b>Epitope Tag: </b>6His-FLAG</span></span>\n \n </td>\n \n </tr>\n \n </tbody>\n \n </table>\n \n </td>\n \n </tr>\n \n <tr>\n \n <td valign=\"top\"><span style=\"font-family: Arial; \" class=\"Apple-style-span\"><span style=\"font-size: 12pt; \" class=\"Apple-style-span\"><br />\n \n <b><span class=\"Apple-style-span\" style=\"font-family: Arial; \"><span class=\"Apple-style-span\" style=\"font-size: 12pt; \"><b>ACF Chromatin Remodeling Enzyme Complex</b></span></span> Description:</b> Recombinant <em>Drosophila</em> ACF Chromatin Remodeling Enzyme Complex, assembled from Acf1-FLAG and ISWI produced in SF9 cells. ACF is an ATP-dependent chromatin remodeling complex that regulates nucleosome spacing.<br />\n \n <br />\n <p><strong>This is product includes <a href=\"https://www.epicypher.com/5x-acf-remodeling-assay-buffer/\">5x ACF Remodeling Assay Buffer (21-0013)</a></strong></p>\n </span></span>\n \n </td>\n \n </tr>\n \n <tr>\n \n <td valign=\"top\"><span style=\"font-family: Arial; \" class=\"Apple-style-span\"><span style=\"font-size: 12pt; \" class=\"Apple-style-span\"><b><span class=\"Apple-style-span\" style=\"font-family: Arial; \"><span class=\"Apple-style-span\" style=\"font-size: 12pt; \"><b>ACF Chromatin Remodeling Enzyme Complex</b></span></span> Formulation:</b> ACF Complex at 0.18 mg/ml in 5.2 μl of 20 mM Tris HCl pH 7.9, 150 mM NaCl, 2 mM MgCl2, 0.2 mM EDTA, 10 mM beta-glycerophosphate, 0.01% NP-40, 0.2 mM PMSF, 0.5 mM benzamidine, 1 mM DTT, 15% glycerol, 2 μg/ml leupeptin, 1 μg/ml aprotinin, 0.4 mg/ml recombinant human insulin, 0.4 mg/ml 3x FLAG peptide. Molarity: 973 nM<br />\n \n <br />\n \n </span></span>\n \n </td>\n \n </tr>\n \n <tr>\n \n <td valign=\"top\"><span style=\"font-family: Arial; \" class=\"Apple-style-span\"><span style=\"font-size: 12pt; \" class=\"Apple-style-span\"><b><b>ACF Chromatin Remodeling Enzyme Complex</b> Storage and Stability:</b> Stable for six months at -80°C from date of receipt. For best results, aliquot and avoid multiple freeze/thaws.<br />\n \n <br />\n \n </span></span>\n \n </td>\n \n </tr>\n \n <tr>\n \n <td valign=\"top\"><span style=\"font-family: Arial; \" class=\"Apple-style-span\"><span style=\"font-size: 12pt; \" class=\"Apple-style-span\"><b><b>ACF Chromatin Remodeling Enzyme Complex</b> Application Notes:</b> This product is sufficient to perform 100 remodeling reactions using EpiDyne®-FRET substrate. A single reaction is defined as 10 μl containing [5 nM ACF Complex/ 20 nM EpiDyne®-FRET/ 50 μM rATP] and remodels to completion in <20 minutes. 5x ACF Remodeling Assay Buffer is included (100 mM Tris HCl, pH 7.5, 250 mM KCl, 15 mM MgCl2, 0.05% (w/v) BSA, 0.05% (v/v) Tween 20). For best results, aliquot ACF into single-use aliquots upon arrival. Avoid multiple freeze/thaw cycles. For further information, please contact [email protected] or refer to the EpiDyne®-FRET Technote (TN#106; available at https://www.epicypher.com/technotes).<br />\n \n </span></span>\n \n </td>\n \n </tr>\n \n <tr>\n \n <td valign=\"top\">\n \n <p><span style=\"font-family: Arial; \" class=\"Apple-style-span\"><span style=\"font-size: 12pt; \" class=\"Apple-style-span\"><b>References:</b><br />\n \n Fyodorov DV & Kadonaga JT (2002) Nature 418: 897-900 (PMID 12192415)<br />\n \n </span></span><br />\n \n </p>\n \n </td>\n \n </tr>\n \n <tr>\n \n <td valign=\"top\"><span style=\"font-family: Arial; \" class=\"Apple-style-span\"><span style=\"font-size: 12pt; \" class=\"Apple-style-span\">View technical datasheet for this product. <a href=\"https://www.epicypher.com/content/documents/tds/15-1013.pdf\" target=\"_blank\"><img src=\"https://cdn7.bigcommerce.com/s-y9o92/content/documents/tds/icon.png\" alt=\"15-1013 Datasheet\" width=\"30\" height=\"40\" /></a></span></span>\n \n </td>\n \n </tr>\n \n </tbody>\n \n </table>\n \n </td>\n \n <td>\n \n <table width=\"100%\" border=\"0\" cellspacing=\"3\" cellpadding=\"3\">\n \n <tbody>\n \n <tr>\n \n <td align=\"center\"><a href=\"https://www.epicypher.com/content/images/products/proteins/15_1013_ATP_dep_chromatin_remodeling.jpg\" target=\"_new\">\n \n <img src=\"https://cdn7.bigcommerce.com/s-y9o92/content/images/products/proteins/15_1013_ATP_dep_chromatin_remodeling.jpg\" alt=\"15-1013 ATP-dependent Chromatin Remodeling \" width=\"250\" height=\"183\" /></a>\n \n </td>\n \n </tr>\n \n <tr>\n \n <td align=\"left\" valign=\"top\">\n \n <p><span style=\"font-family: Arial; \" class=\"Apple-style-span\"><span style=\"font-size: 10pt; \" class=\"Apple-style-span\"><strong>ATP-dependent Chromatin Remodeling Assay:</strong> EpiDyne®-FRET Chromatin Remodeling Substrate (20 nM; Catalog No. 16-4201) incubated with ACF Chromatin Remodeling Enzyme Complex (concentrations indicated), with or without ATP (50 μM). Curves denote FRET efficiency/chromatin remodeling.<br />\n \n <strong>(Click image to enlarge) </strong></span></span> </p></td>\n \n </tr>\n \n </tbody>\n \n </table>\n \n \n \n \n <table width=\"100%\" border=\"0\" cellspacing=\"3\" cellpadding=\"3\">\n \n <tbody>\n \n \n \n </tbody>\n \n </table>\n \n </td>\n \n </tr>\n \n </tbody>\n\n</table>\n\n\n\n\n</body>\n</html>\n","detail_messages":"","gift_wrapping_available":false,"gtin":null,"id":"551","images":[{"alt":"ACF Chromatin Remodeling Enzyme Complex","data":"https://cdn11.bigcommerce.com/s-y9o92/images/stencil/{:size}/products/551/597/Untitled__22497.1556128753.png?c=2"}],"main_image":{"alt":"ACF Chromatin Remodeling Enzyme Complex","data":"https://cdn11.bigcommerce.com/s-y9o92/images/stencil/{:size}/products/551/597/Untitled__22497.1556128753.png?c=2"},"max_purchase_quantity":0,"meta_description":"ACF Remodeling Enzyme Complex, ACF Remodeling Enzyme Complex, ACF, Remodeling Enzyme Complex, chromatin remodeling complex, EpiDyne control, EpiDyne, ","meta_keywords":"ACF Remodeling Enzyme Complex, ACF Remodeling Enzyme Complex, ACF, Remodeling Enzyme Complex, chromatin remodeling complex, EpiDyne control, EpiDyne, ","min_purchase_quantity":0,"mpn":null,"num_reviews":0,"options":[],"page_title":"ACF Remodeling Enzyme Complex","price":{"tax_label":"Sales Tax","without_tax":{"currency":"USD","formatted":"$505.00","value":505}},"rating":0,"related_products":[{"add_to_cart_url":"https://www.epicypher.com/cart.php?action=add&product_id=623","add_to_wishlist_url":"/wishlist.php?action=add&product_id=623","availability":"","brand":{"name":null},"category":["Recombinant Proteins/Modifying Enzymes"],"custom_fields":[{"id":587,"name":"Pack Size","value":"500 µL"}],"date_added":"21st Sep 2018","demo":false,"has_options":false,"id":623,"image":{"alt":"5x ACF Remodeling Assay Buffer","data":"https://cdn11.bigcommerce.com/s-y9o92/images/stencil/{:size}/products/623/598/Untitled__68407.1556128753.png?c=2"},"images":[{"alt":"5x 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Proteins/EpiDyne<sup>®</sup> Chromatin Remodeling Enzymes","Recombinant Proteins/Modifying Enzymes"],"custom_fields":[{"id":1081,"name":"Pack Size","value":"100 Reactions"},{"id":1082,"name":"Internal Comment","value":"extra in top right of Psylocke"}],"date_added":"15th Feb 2019","demo":false,"has_options":false,"id":650,"image":{"alt":"SMARCA2 Chromatin Remodeling Enzyme Human BRM","data":"https://cdn11.bigcommerce.com/s-y9o92/images/stencil/{:size}/products/650/953/smarca2-chromatin-remodeling-enzyme-human-brm__84445.1645734545.jpg?c=2"},"images":[{"alt":"SMARCA2 Chromatin Remodeling Enzyme Human BRM","data":"https://cdn11.bigcommerce.com/s-y9o92/images/stencil/{:size}/products/650/953/smarca2-chromatin-remodeling-enzyme-human-brm__84445.1645734545.jpg?c=2"}],"low_stock_level":null,"name":"SMARCA2 Chromatin Remodeling Enzyme (Human BRM)","num_reviews":null,"pre_order":false,"price":{"tax_label":"Sales Tax","without_tax":{"currency":"USD","formatted":"$550.00","value":550}},"qty_in_cart":0,"rating":null,"show_cart_action":true,"sku":"15-1015","stock_level":null,"summary":"\n \n \n Species: Human\n \n \n Source: SF9 cells\n \n \n \n ","url":"https://www.epicypher.com/products/nucleosomes/epidyne-chromatin-remodeling-substrates/epidyne-modifying-enzymes/smarca2-chromatin-remodeling-enzyme-human-brm","weight":{"formatted":"0.01 LBS","value":0.01}},{"add_to_cart_url":"https://www.epicypher.com/cart.php?action=add&product_id=651","add_to_wishlist_url":"/wishlist.php?action=add&product_id=651","availability":"","brand":{"name":null},"category":["Nucleosomes/EpiDyne® Chromatin Remodeling Substrates/EpiDyne<sup>®</sup> Modifying Enzymes ","Recombinant Proteins/EpiDyne<sup>®</sup> Chromatin Remodeling Enzymes","Recombinant Proteins/Modifying Enzymes"],"custom_fields":[{"id":1140,"name":"Pack size","value":"100 reactions"},{"id":1141,"name":"Internal Comment","value":"21-1014 is sold with this item."},{"id":1142,"name":"Internal Comment","value":"51 units of R&D Lot 7 are in HQ"},{"id":1143,"name":"Internal Comment","value":"Bulk in Protein Box in LG, Lot 7"}],"date_added":"15th Feb 2019","demo":false,"has_options":false,"id":651,"image":{"alt":"SMARCA4 Chromatin Remodeling Enzyme (Human BRG1)","data":"https://cdn11.bigcommerce.com/s-y9o92/images/stencil/{:size}/products/651/616/Untitled__68998.1556128740.png?c=2"},"images":[{"alt":"SMARCA4 Chromatin Remodeling Enzyme (Human BRG1)","data":"https://cdn11.bigcommerce.com/s-y9o92/images/stencil/{:size}/products/651/616/Untitled__68998.1556128740.png?c=2"}],"low_stock_level":null,"name":"SMARCA4 Chromatin Remodeling Enzyme (Human BRG1)","num_reviews":null,"pre_order":false,"price":{"tax_label":"Sales Tax","without_tax":{"currency":"USD","formatted":"$550.00","value":550}},"qty_in_cart":0,"rating":null,"show_cart_action":true,"sku":"15-1014","stock_level":null,"summary":"\n \n \n Species: Human\n \n \n Source: SF9 cells\n \n \n \n ","url":"https://www.epicypher.com/products/nucleosomes/epidyne-chromatin-remodeling-substrates/epidyne-modifying-enzymes/smarca4-chromatin-remodeling-enzyme-human-brg1","weight":{"formatted":"0.01 LBS","value":0.01}},{"add_to_cart_url":"https://www.epicypher.com/cart.php?action=add&product_id=876","add_to_wishlist_url":"/wishlist.php?action=add&product_id=876","availability":"","brand":{"name":null},"category":["Recombinant Proteins/Modifying Enzymes"],"custom_fields":[{"id":913,"name":"Pack Size","value":"500 µL"}],"date_added":"22nd Jul 2021","demo":false,"has_options":false,"id":876,"image":{"alt":"5x SMARCA Remodeling Assay Buffer","data":"https://cdn11.bigcommerce.com/s-y9o92/images/stencil/{:size}/products/876/941/5x-smarca-remodeling-assay-buffer__61629.1645734533.jpg?c=2"},"images":[{"alt":"5x SMARCA Remodeling Assay Buffer","data":"https://cdn11.bigcommerce.com/s-y9o92/images/stencil/{:size}/products/876/941/5x-smarca-remodeling-assay-buffer__61629.1645734533.jpg?c=2"}],"low_stock_level":null,"name":"5x SMARCA Remodeling Assay Buffer","num_reviews":null,"pre_order":false,"price":{"tax_label":"Sales Tax","without_tax":{"currency":"USD","formatted":"$55.00","value":55}},"qty_in_cart":0,"rating":null,"show_cart_action":true,"sku":"21-0014","stock_level":null,"summary":"\n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n ","url":"https://www.epicypher.com/products/recombinant-proteins/modifying-enzymes/5x-smarca-remodeling-assay-buffer","weight":{"formatted":"0.01 LBS","value":0.01}},{"add_to_cart_url":"https://www.epicypher.com/cart.php?action=add&product_id=922","add_to_wishlist_url":"/wishlist.php?action=add&product_id=922","availability":"","brand":{"name":null},"category":["Nucleosomes/EpiDyne® Chromatin Remodeling Substrates/EpiDyne<sup>®</sup> Modifying Enzymes ","Recombinant Proteins/EpiDyne<sup>®</sup> Chromatin Remodeling Enzymes","Recombinant Proteins/Modifying Enzymes"],"custom_fields":[{"id":1109,"name":"Pack Size","value":"100 Reactions"}],"date_added":"23rd Jun 2022","demo":false,"has_options":false,"id":922,"image":{"alt":"SMARCA5 Chromatin Remodeling Enzyme (Human SNF2H)","data":"https://cdn11.bigcommerce.com/s-y9o92/images/stencil/{:size}/products/922/1026/smarca2-chromatin-remodeling-enzyme-human-brm__84445.1645734545.1280.1280.jpg_c_2%26_gl_1_1rzfkza__ga_MTc4NjU1MDYxMi4xNjU1MzE1MzYx__ga_WS2VZYPC6G_MTY1NTk5NzEwNy4yMy4xLjE2NTU5OTcxMzEuMzY__08994.1655997178.jpg?c=2"},"images":[{"alt":"SMARCA5 Chromatin Remodeling Enzyme (Human SNF2H)","data":"https://cdn11.bigcommerce.com/s-y9o92/images/stencil/{:size}/products/922/1026/smarca2-chromatin-remodeling-enzyme-human-brm__84445.1645734545.1280.1280.jpg_c_2%26_gl_1_1rzfkza__ga_MTc4NjU1MDYxMi4xNjU1MzE1MzYx__ga_WS2VZYPC6G_MTY1NTk5NzEwNy4yMy4xLjE2NTU5OTcxMzEuMzY__08994.1655997178.jpg?c=2"}],"low_stock_level":null,"name":"SMARCA5 Chromatin Remodeling Enzyme (Human SNF2H)","num_reviews":null,"pre_order":false,"price":{"tax_label":"Sales Tax","without_tax":{"currency":"USD","formatted":"$405.00","value":405}},"qty_in_cart":0,"rating":null,"show_cart_action":true,"sku":"15-1024","stock_level":null,"summary":"\n \n \n Species: Human\n \n \n Source: SF9 cells\n \n \n \n ","url":"https://www.epicypher.com/products/nucleosomes/epidyne-chromatin-remodeling-substrates/epidyne-sup-sup-modifying-enzymes/smarca5-chromatin-remodeling-enzyme-human-snf2h","weight":{"formatted":"0.01 LBS","value":0.01}}],"shipping":{"calculated":true},"shipping_messages":[],"show_quantity_input":1,"sku":"15-1013","tags":[],"title":"ACF Chromatin Remodeling Enzyme Complex","upc":null,"url":"https://www.epicypher.com/products/nucleosomes/epidyne-chromatin-remodeling-substrates/epidyne-modifying-enzymes/acf-chromatin-remodeling-enzyme-complex","warranty":"","weight":"0.01 LBS"} Pack Size: 100 reactions
{"AddThisServiceButtonMeta":"","add_this":[{"annotation":"","service":"facebook"},{"annotation":"","service":"email"},{"annotation":"","service":"print"},{"annotation":"","service":"twitter"},{"annotation":"","service":"linkedin"}],"add_to_wishlist_url":"/wishlist.php?action=add&product_id=551","availability":"","bulk_discount_rates":[],"can_purchase":true,"cart_url":"https://www.epicypher.com/cart.php","category":["Nucleosomes/EpiDyne® Chromatin Remodeling Substrates/EpiDyne<sup>®</sup> Modifying Enzymes ","Recombinant Proteins/EpiDyne<sup>®</sup> Chromatin Remodeling Enzymes","Recombinant Proteins/Modifying Enzymes"],"custom_fields":[{"id":"585","name":"Pack Size","value":"100 reactions"}],"customizations":[],"description":"<table width=\"100%\" border=\"0\" cellspacing=\"2\" cellpadding=\"2\">\n \n <tbody>\n \n <tr valign=\"top\">\n \n <td>\n \n \n \n <table width=\"95%\" border=\"0\" cellspacing=\"1\" cellpadding=\"1\">\n \n <tbody>\n \n <tr>\n \n <td>\n \n <table width=\"100%\" border=\"0\" cellspacing=\"0\" cellpadding=\"0\">\n \n <tbody>\n \n <tr>\n \n <td align=\"left\" valign=\"top\"><span style=\"font-family: Arial; \" class=\"Apple-style-span\"><span style=\"font-size: 10pt; \" class=\"Apple-style-span\"><b>Type: </b>Remodeler/ATPase</span></span>\n \n </td>\n \n <td width=\"196\" align=\"left\" valign=\"top\"><span style=\"font-family: Arial; \" class=\"Apple-style-span\"><span style=\"font-size: 10pt; \" class=\"Apple-style-span\"><b>Host: </b>SF9 cells</span></span>\n \n </td>\n \n </tr>\n \n <tr>\n \n <td align=\"left\" valign=\"top\"><span style=\"font-family: Arial; \" class=\"Apple-style-span\"><span style=\"font-size: 10pt; \" class=\"Apple-style-span\"><b>Mol Wgt.: </b>185 kDa</span></span>\n \n </td>\n \n <td align=\"left\" valign=\"top\"><span style=\"font-family: Arial; \" class=\"Apple-style-span\"><span style=\"font-size: 10pt; \" class=\"Apple-style-span\"><b>Epitope Tag: </b>6His-FLAG</span></span>\n \n </td>\n \n </tr>\n \n </tbody>\n \n </table>\n \n </td>\n \n </tr>\n \n <tr>\n \n <td valign=\"top\"><span style=\"font-family: Arial; \" class=\"Apple-style-span\"><span style=\"font-size: 12pt; \" class=\"Apple-style-span\"><br />\n \n <b><span class=\"Apple-style-span\" style=\"font-family: Arial; \"><span class=\"Apple-style-span\" style=\"font-size: 12pt; \"><b>ACF Chromatin Remodeling Enzyme Complex</b></span></span> Description:</b> Recombinant <em>Drosophila</em> ACF Chromatin Remodeling Enzyme Complex, assembled from Acf1-FLAG and ISWI produced in SF9 cells. ACF is an ATP-dependent chromatin remodeling complex that regulates nucleosome spacing.<br />\n \n <br />\n <p><strong>This is product includes <a href=\"https://www.epicypher.com/5x-acf-remodeling-assay-buffer/\">5x ACF Remodeling Assay Buffer (21-0013)</a></strong></p>\n </span></span>\n \n </td>\n \n </tr>\n \n <tr>\n \n <td valign=\"top\"><span style=\"font-family: Arial; \" class=\"Apple-style-span\"><span style=\"font-size: 12pt; \" class=\"Apple-style-span\"><b><span class=\"Apple-style-span\" style=\"font-family: Arial; \"><span class=\"Apple-style-span\" style=\"font-size: 12pt; \"><b>ACF Chromatin Remodeling Enzyme Complex</b></span></span> Formulation:</b> ACF Complex at 0.18 mg/ml in 5.2 μl of 20 mM Tris HCl pH 7.9, 150 mM NaCl, 2 mM MgCl2, 0.2 mM EDTA, 10 mM beta-glycerophosphate, 0.01% NP-40, 0.2 mM PMSF, 0.5 mM benzamidine, 1 mM DTT, 15% glycerol, 2 μg/ml leupeptin, 1 μg/ml aprotinin, 0.4 mg/ml recombinant human insulin, 0.4 mg/ml 3x FLAG peptide. Molarity: 973 nM<br />\n \n <br />\n \n </span></span>\n \n </td>\n \n </tr>\n \n <tr>\n \n <td valign=\"top\"><span style=\"font-family: Arial; \" class=\"Apple-style-span\"><span style=\"font-size: 12pt; \" class=\"Apple-style-span\"><b><b>ACF Chromatin Remodeling Enzyme Complex</b> Storage and Stability:</b> Stable for six months at -80°C from date of receipt. For best results, aliquot and avoid multiple freeze/thaws.<br />\n \n <br />\n \n </span></span>\n \n </td>\n \n </tr>\n \n <tr>\n \n <td valign=\"top\"><span style=\"font-family: Arial; \" class=\"Apple-style-span\"><span style=\"font-size: 12pt; \" class=\"Apple-style-span\"><b><b>ACF Chromatin Remodeling Enzyme Complex</b> Application Notes:</b> This product is sufficient to perform 100 remodeling reactions using EpiDyne®-FRET substrate. A single reaction is defined as 10 μl containing [5 nM ACF Complex/ 20 nM EpiDyne®-FRET/ 50 μM rATP] and remodels to completion in <20 minutes. 5x ACF Remodeling Assay Buffer is included (100 mM Tris HCl, pH 7.5, 250 mM KCl, 15 mM MgCl2, 0.05% (w/v) BSA, 0.05% (v/v) Tween 20). For best results, aliquot ACF into single-use aliquots upon arrival. Avoid multiple freeze/thaw cycles. For further information, please contact [email protected] or refer to the EpiDyne®-FRET Technote (TN#106; available at https://www.epicypher.com/technotes).<br />\n \n </span></span>\n \n </td>\n \n </tr>\n \n <tr>\n \n <td valign=\"top\">\n \n <p><span style=\"font-family: Arial; \" class=\"Apple-style-span\"><span style=\"font-size: 12pt; \" class=\"Apple-style-span\"><b>References:</b><br />\n \n Fyodorov DV & Kadonaga JT (2002) Nature 418: 897-900 (PMID 12192415)<br />\n \n </span></span><br />\n \n </p>\n \n </td>\n \n </tr>\n \n <tr>\n \n <td valign=\"top\"><span style=\"font-family: Arial; \" class=\"Apple-style-span\"><span style=\"font-size: 12pt; \" class=\"Apple-style-span\">View technical datasheet for this product. <a href=\"https://www.epicypher.com/content/documents/tds/15-1013.pdf\" target=\"_blank\"><img src=\"https://cdn7.bigcommerce.com/s-y9o92/content/documents/tds/icon.png\" alt=\"15-1013 Datasheet\" width=\"30\" height=\"40\" /></a></span></span>\n \n </td>\n \n </tr>\n \n </tbody>\n \n </table>\n \n </td>\n \n <td>\n \n <table width=\"100%\" border=\"0\" cellspacing=\"3\" cellpadding=\"3\">\n \n <tbody>\n \n <tr>\n \n <td align=\"center\"><a href=\"https://www.epicypher.com/content/images/products/proteins/15_1013_ATP_dep_chromatin_remodeling.jpg\" target=\"_new\">\n \n <img src=\"https://cdn7.bigcommerce.com/s-y9o92/content/images/products/proteins/15_1013_ATP_dep_chromatin_remodeling.jpg\" alt=\"15-1013 ATP-dependent Chromatin Remodeling \" width=\"250\" height=\"183\" /></a>\n \n </td>\n \n </tr>\n \n <tr>\n \n <td align=\"left\" valign=\"top\">\n \n <p><span style=\"font-family: Arial; \" class=\"Apple-style-span\"><span style=\"font-size: 10pt; \" class=\"Apple-style-span\"><strong>ATP-dependent Chromatin Remodeling Assay:</strong> EpiDyne®-FRET Chromatin Remodeling Substrate (20 nM; Catalog No. 16-4201) incubated with ACF Chromatin Remodeling Enzyme Complex (concentrations indicated), with or without ATP (50 μM). Curves denote FRET efficiency/chromatin remodeling.<br />\n \n <strong>(Click image to enlarge) </strong></span></span> </p></td>\n \n </tr>\n \n </tbody>\n \n </table>\n \n \n \n \n <table width=\"100%\" border=\"0\" cellspacing=\"3\" cellpadding=\"3\">\n \n <tbody>\n \n \n \n </tbody>\n \n </table>\n \n </td>\n \n </tr>\n \n </tbody>\n\n</table>\n\n\n\n\n</body>\n</html>\n","detail_messages":"","gift_wrapping_available":false,"gtin":null,"id":"551","images":[{"alt":"ACF Chromatin Remodeling Enzyme Complex","data":"https://cdn11.bigcommerce.com/s-y9o92/images/stencil/{:size}/products/551/597/Untitled__22497.1556128753.png?c=2"}],"main_image":{"alt":"ACF Chromatin Remodeling Enzyme Complex","data":"https://cdn11.bigcommerce.com/s-y9o92/images/stencil/{:size}/products/551/597/Untitled__22497.1556128753.png?c=2"},"max_purchase_quantity":0,"meta_description":"ACF Remodeling Enzyme Complex, ACF Remodeling Enzyme Complex, ACF, Remodeling Enzyme Complex, chromatin remodeling complex, EpiDyne 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