ACF Chromatin Remodeling Enzyme Complex

$505.00

SKU: 15-1013
{"AddThisServiceButtonMeta":"","add_this":[{"annotation":"","service":"facebook"},{"annotation":"","service":"email"},{"annotation":"","service":"print"},{"annotation":"","service":"twitter"},{"annotation":"","service":"linkedin"}],"add_to_wishlist_url":"/wishlist.php?action=add&product_id=551","availability":"","bulk_discount_rates":[],"can_purchase":true,"cart_url":"https://www.epicypher.com/cart.php","category":["Nucleosomes/EpiDyne® Chromatin Remodeling Substrates/EpiDyne® Modifying Enzymes ","Recombinant Proteins/EpiDyne® Chromatin Remodeling Enzymes","Recombinant Proteins/Modifying Enzymes"],"custom_fields":[{"id":"585","name":"Pack Size","value":"100 reactions"}],"customizations":[],"description":"<table width=\"100%\" border=\"0\" cellspacing=\"2\" cellpadding=\"2\">\n \n <tbody>\n \n <tr valign=\"top\">\n \n <td>\n \n \n \n <table width=\"95%\" border=\"0\" cellspacing=\"1\" cellpadding=\"1\">\n \n <tbody>\n \n <tr>\n \n <td>\n \n <table width=\"100%\" border=\"0\" cellspacing=\"0\" cellpadding=\"0\">\n \n <tbody>\n \n <tr>\n \n <td align=\"left\" valign=\"top\">Type:  Remodeler/ATPase\n \n </td>\n \n <td width=\"196\" align=\"left\" valign=\"top\">Host: SF9 cells\n \n </td>\n \n </tr>\n \n <tr>\n \n <td align=\"left\" valign=\"top\">Mol Wgt.: 185 kDa\n \n </td>\n \n <td align=\"left\" valign=\"top\">Epitope Tag:  6His-FLAG\n \n </td>\n \n </tr>\n \n </tbody>\n \n </table>\n \n </td>\n \n </tr>\n \n <tr>\n \n <td valign=\"top\"> \n \n ACF Chromatin Remodeling Enzyme Complex Description: Recombinant Drosophila ACF Chromatin Remodeling Enzyme Complex, assembled from Acf1-FLAG and ISWI produced in SF9 cells. ACF is an ATP-dependent chromatin remodeling complex that regulates nucleosome spacing. \n \n \n This is product includes <a href=\"https://www.epicypher.com/5x-acf-remodeling-assay-buffer/\">5x ACF Remodeling Assay Buffer (21-0013)</a>\n \n \n </td>\n \n </tr>\n \n <tr>\n \n <td valign=\"top\">ACF Chromatin Remodeling Enzyme Complex Formulation: ACF Complex at 0.18 mg/ml in 5.2 μl of 20 mM Tris HCl pH 7.9, 150 mM NaCl, 2 mM MgCl2, 0.2 mM EDTA, 10 mM beta-glycerophosphate, 0.01% NP-40, 0.2 mM PMSF, 0.5 mM benzamidine, 1 mM DTT, 15% glycerol, 2 μg/ml leupeptin, 1 μg/ml aprotinin, 0.4 mg/ml recombinant human insulin, 0.4 mg/ml 3x FLAG peptide. Molarity: 973 nM \n \n \n \n \n \n </td>\n \n </tr>\n \n <tr>\n \n <td valign=\"top\">ACF Chromatin Remodeling Enzyme Complex Storage and Stability: Stable for six months at -80°C from date of receipt. For best results, aliquot and avoid multiple freeze/thaws. \n \n \n \n \n \n </td>\n \n </tr>\n \n <tr>\n \n <td valign=\"top\">ACF Chromatin Remodeling Enzyme Complex Application Notes: This product is sufficient to perform 100 remodeling reactions using EpiDyne®-FRET substrate. A single reaction is defined as 10 μl containing [5 nM ACF Complex/ 20 nM EpiDyne®-FRET/ 50 μM rATP] and remodels to completion in <20 minutes. 5x ACF Remodeling Assay Buffer is included (100 mM Tris HCl, pH 7.5, 250 mM KCl, 15 mM MgCl2, 0.05% (w/v) BSA, 0.05% (v/v) Tween 20). For best results, aliquot ACF into single-use aliquots upon arrival. Avoid multiple freeze/thaw cycles. For further information, please contact techsupport@epicypher.com or refer to the EpiDyne®-FRET Technote (TN#106; available at https://www.epicypher.com/technotes). \n \n \n \n </td>\n \n </tr>\n \n <tr>\n \n <td valign=\"top\">\n \n References: \n \n Fyodorov DV & Kadonaga JT (2002) Nature 418: 897-900 (PMID 12192415) \n \n \n \n \n \n </td>\n \n </tr>\n \n <tr>\n \n <td valign=\"top\">View technical datasheet for this product. <a href=\"https://www.epicypher.com/content/documents/tds/15-1013.pdf\" target=\"_blank\"><img src=\"https://cdn7.bigcommerce.com/s-y9o92/content/documents/tds/icon.png\" alt=\"15-1013 Datasheet\" width=\"30\" height=\"40\" /></a>\n \n </td>\n \n </tr>\n \n </tbody>\n \n </table>\n \n </td>\n \n <td>\n \n <table width=\"100%\" border=\"0\" cellspacing=\"3\" cellpadding=\"3\">\n \n <tbody>\n \n <tr>\n \n <td align=\"center\"><a href=\"https://www.epicypher.com/content/images/products/proteins/15_1013_ATP_dep_chromatin_remodeling.jpg\" target=\"_new\">\n \n <img src=\"https://cdn7.bigcommerce.com/s-y9o92/content/images/products/proteins/15_1013_ATP_dep_chromatin_remodeling.jpg\" alt=\"15-1013 ATP-dependent Chromatin Remodeling \" width=\"250\" height=\"183\" /></a>\n \n </td>\n \n </tr>\n \n <tr>\n \n <td align=\"left\" valign=\"top\">\n \n ATP-dependent Chromatin Remodeling Assay: EpiDyne®-FRET Chromatin Remodeling Substrate (20 nM; Catalog No. 16-4201) incubated with ACF Chromatin Remodeling Enzyme Complex (concentrations indicated), with or without ATP (50 μM). Curves denote FRET efficiency/chromatin remodeling. \n \n (Click image to enlarge) </td>\n \n </tr>\n \n </tbody>\n \n </table>\n \n \n \n \n <table width=\"100%\" border=\"0\" cellspacing=\"3\" cellpadding=\"3\">\n \n <tbody>\n \n \n \n </tbody>\n \n </table>\n \n </td>\n \n </tr>\n \n </tbody>\n\n</table>\n\n\n\n\n</body>\n</html>\n","detail_messages":"","gift_wrapping_available":false,"id":"551","images":[{"alt":"ACF Chromatin Remodeling Enzyme Complex","data":"https://cdn11.bigcommerce.com/s-y9o92/images/stencil/{:size}/products/551/597/Untitled__22497.1556128753.png?c=2"}],"main_image":{"alt":"ACF Chromatin Remodeling Enzyme Complex","data":"https://cdn11.bigcommerce.com/s-y9o92/images/stencil/{:size}/products/551/597/Untitled__22497.1556128753.png?c=2"},"max_purchase_quantity":0,"meta_description":"ACF Remodeling Enzyme Complex, ACF Remodeling Enzyme Complex, ACF, Remodeling Enzyme Complex, chromatin remodeling complex, EpiDyne control, EpiDyne, ","meta_keywords":"ACF Remodeling 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Type: Remodeler/ATPase	Host: SF9 cells
Mol Wgt.: 185 kDa	Epitope Tag: 6His-FLAG

ACF Chromatin Remodeling Enzyme Complex Description: Recombinant Drosophila ACF Chromatin Remodeling Enzyme Complex, assembled from Acf1-FLAG and ISWI produced in SF9 cells. ACF is an ATP-dependent chromatin remodeling complex that regulates nucleosome spacing.

This is product includes 5x ACF Remodeling Assay Buffer (21-0013)

ACF Chromatin Remodeling Enzyme Complex Formulation: ACF Complex at 0.18 mg/ml in 5.2 μl of 20 mM Tris HCl pH 7.9, 150 mM NaCl, 2 mM MgCl2, 0.2 mM EDTA, 10 mM beta-glycerophosphate, 0.01% NP-40, 0.2 mM PMSF, 0.5 mM benzamidine, 1 mM DTT, 15% glycerol, 2 μg/ml leupeptin, 1 μg/ml aprotinin, 0.4 mg/ml recombinant human insulin, 0.4 mg/ml 3x FLAG peptide. Molarity: 973 nM

ACF Chromatin Remodeling Enzyme Complex Storage and Stability: Stable for six months at -80°C from date of receipt. For best results, aliquot and avoid multiple freeze/thaws.

ACF Chromatin Remodeling Enzyme Complex Application Notes: This product is sufficient to perform 100 remodeling reactions using EpiDyne®-FRET substrate. A single reaction is defined as 10 μl containing [5 nM ACF Complex/ 20 nM EpiDyne®-FRET/ 50 μM rATP] and remodels to completion in <20 minutes. 5x ACF Remodeling Assay Buffer is included (100 mM Tris HCl, pH 7.5, 250 mM KCl, 15 mM MgCl2, 0.05% (w/v) BSA, 0.05% (v/v) Tween 20). For best results, aliquot ACF into single-use aliquots upon arrival. Avoid multiple freeze/thaw cycles. For further information, please contact techsupport@epicypher.com or refer to the EpiDyne®-FRET Technote (TN#106; available at https://www.epicypher.com/technotes).

References:
Fyodorov DV & Kadonaga JT (2002) Nature 418: 897-900 (PMID 12192415)

View technical datasheet for this product.

15-1013 ATP-dependent Chromatin Remodeling

ATP-dependent Chromatin Remodeling Assay: EpiDyne®-FRET Chromatin Remodeling Substrate (20 nM; Catalog No. 16-4201) incubated with ACF Chromatin Remodeling Enzyme Complex (concentrations indicated), with or without ATP (50 μM). Curves denote FRET efficiency/chromatin remodeling.
(Click image to enlarge)

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