SMARCA5 Chromatin Remodeling Enzyme (Human SNF2H)

SKU: 15-1024
Pack Size: 100 Reactions
  • Species:  Human
  • Source:  SF9 cells
  • Epitope Tag:  FLAG-6His
  • Mol Wgt:  123.7 kDa


Full length recombinant human SMARCA5 Remodeling Enzyme. SMARCA5 is a core ATPase subunit of the ATP-dependent chromatin remodeling ISWI complexes that regulate nucleosome spacing to control DNA accessibility [1,2]. SMARCA5 containing complexes exhibit substrate preference and distinct remodeling directionalities with different regulatory subunits [3]. Mis-regulation of SMARCA5 is linked with multiple human cancers including leukemia, breast, lung, and gastric cancers [4]. SMARCA5 can be used in conjunction with EpiDyne®-FRET Remodeling Assay Substrate (EpiCypher 16-4201) in HTS-compatible drug discovery studies.

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Validation Data

Figure 1: ATP-dependent chromatin remodeling assay
EpiDyne®-FRET Chromatin Remodeling Substrate (EpiCypher 16-4201; 20nM) incubated with SMARCA5 Remodeling Enzyme (concentrations indicated) in 1x SMARCA Remodeling Assay Buffer. Curves denote FRET efficiency/chromatin remodeling.

Figure 2: Protein gel data
SMARCA5 Remodeling Enzyme (1 µg) was run on an SDS-PAGE gel and stained with Coomassie blue. The migration and molecular weight of the protein standards are indicated.

Technical Information

25 mM HEPES pH 7.6, 0.1 mM EDTA, 10% glycerol, 200 mM NaCl, 1 mM DTT, 0.4 mM PMSF, 1 mM benzamidine, 0.02% NP-40, 0.4 mg/mL insulin.
Storage and Stability:
Stable for six months -80°C from date of receipt. For best results, aliquot and avoid multiple freeze/thaws.

Application Notes

This product is sufficient to perform 100 remodeling reactions using EpiDyne®-FRET Substrate (EpiCypher 16-4201). A single reaction is defined as 10 µL containing a lot-specific concentration of SMARCA5 (see Technical Datasheet), 20 nM EpiDyne®-FRET, & 1 mM rATP with remodeling to completion in <30 minutes. Remodeling readout can be observed in the change of Cy3/Cy5 ratio over time in a black 384-well assay plate on a compatible fluorescent plate reader. 5x SMARCA Remodeling Assay Buffer is included (EpiCypher 21-0014). For more information, see the EpiDyne®-FRET Technote or contact

Gene & Protein Information

UniProt ID
Gene Name
Protein Name
SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily A member 5
Amino Acids
2-1052 with an N-terminal FLAG-6His
Alternate Names
SNF2H, WCRF135, Sucrose nonfermenting protein 2 homolog


Background References:
[1] Clapier & Carins Annu. Rev. Biochem. (2009). PMID: 19355820
[2] Sundaramoorthy & Owen-Hughes F1000Research (2020). PMID: 32864100
[3] Oppikofer et al. EMBO Rep. (2017). PMID: 28801535
[4] Thakur et al. Cells (2022). PMID: 35269430

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