SMARCA4

SMARCA4 Chromatin Remodeling Enzyme (Human BRG1)

$550.00
SKU: 15-1014
Pack size: 100 reactions
  • Species:  Human
  • Source:  SF9 cells
  • Epitope Tag:  6xHis-FLAG
  • Mol Wgt:  186.6 kDa

Description

Full length recombinant human SMARCA4 Remodeling Enzyme. SMARCA4 is a core ATPase subunit of the ATP-dependent chromatin remodeling complex SWI/SNF that mobilizes nucleosomes and plays key roles in gene transcription regulation, development, genome replication and repair [1,2]. Aberrant activity of SWI/SNF subunits is linked with more than 20% of human cancers and multiple developmental disorders, making SMARCA4 an emerging candidate therapeutic target [3,4]. SMARCA4 can be used in conjunction with EpiDyne®-FRET Remodeling Assay Substrate (EpiCypher 16-4201) in HTS-compatible drug discovery studies.

Validation Data

Figure 1: ATP-dependent chromatin remodeling assay
EpiDyne®-FRET Chromatin Remodeling Substrate (EpiCypher 16-4201; 20nM) incubated with SMARCA4 Remodeling Enzyme (concentrations indicated) in 1x SMARCA Remodeling Assay Buffer. Curves denote FRET efficiency/chromatin remodeling.

Figure 2: Protein gel data
SMARCA4 Remodeling Enzyme (1 µg) was run on an SDS-PAGE gel and stained with Coomassie blue. The migration and molecular weight of the protein standards are indicated.

Technical Information

Formulation:
25 mM HEPES pH 7.6, 0.1 mM EDTA, 10% glycerol, 350 mM NaCl, 1 mM DTT, 0.4 mM PMSF, 1 mM benzamidine, 0.02% NP-40, 0.4 mg/mL insulin.
Storage and Stability:
Stable for six months -80°C from date of receipt. For best results, aliquot and avoid multiple freeze/thaws.

Application Notes

This product is sufficient to perform 100 remodeling reactions using EpiDyne® -FRET substrate (EpiCypher 16-4201). A single reaction is defined as 10 µL containing a lot-specific concentration of SMARCA4 (see Technical Datasheet), 20 nM EpiDyne® -FRET, & 1 mM rATP with remodeling to completion in <30 minutes. Remodeling readout can be observed in the change of Cy3/Cy5 ratio over time in a black 384-well assay plate on a compatible fluorescent plate reader. 5x SMARCA Remodeling Assay Buffer is included (EpiCypher 21-0014). For more information, see the EpiDyne®-FRET Technote or contact techsupport@epicypher.com.

Gene & Protein Information

UniProt ID
P51532-1
Gene Name
SMARCA4
Protein Name
Transcription activator BRG1
Amino Acids
1-1647 with a C-terminal 6His-FLAG
Alternate Names
BAF190A, BRG1, SNF2B, SNF2L4

References

Background References:
[1] Clapier & Carins Annu. Rev. Biochem. (2009). PMID: 19355820
[2] Sundaramoorthy & Owen-Hughes F1000Research (2020). PMID: 32864100
[3] Kadoch & Crabtree Sci. Adv. (2015). PMID: 26601204
[4] Cenik & Shilatifard Cells (2022). PMID: 32958894

Documents & Resources

Current stock: 0