5x SMARCA Remodeling Assay Buffer

SKU: 21-0014
Pack Size: 500 µL
5x SMARCA Remodeling Assay Buffer
5x concentrated reaction buffer for use with SMARCA Remodeling Enzymes (EpiCypher 15-1014 and 15-1015). Optimized for use with SMARCA Remodeling Enzyme, produced in SF9 cells. SMARCA is an ATP-dependent chromatin remodeling complex that regulates nucleosome spacing.
SMARCA Remodeling Enzymes Formulation: 5x SMARCA Remodeling Assay Buffer (100 mM Tris HCl, pH 7.5, 250 mM KCl, 15 mM MgCl2, 0.05% (w/v) BSA, 0.05% (v/v) Tween 20) is formulated for use with SMARCA Remodeling Enzyme.

Storage and Stability: For best results, store 5x SMARCA Remodeling Assay buffer at 4˚C and avoid freezing.

Application Notes: This product is formulated to perform remodeling reactions using SMARCA Remodeling Enzyme and EpiDyne®-FRET substrate (EpiCypher 16-4201) in a 10 μL reaction containing 20 nM SMARCA, 20 nM EpiDyne-FRET, and 2.5 mM rATP. Enzyme remodels to completion in <30 minutes.

For further information, please contact techsupport@epicypher.com or refer to the EpiDyne®- FRET Technote (available at www.epicypher.com/technotes).

View technical datasheet for this product. 21-0014 Datasheet
15-1014 ATP-dependent Chromatin Remodeling

ATP-dependent Chromatin Remodeling Assay: EpiDyne-FRET Chromatin Remodeling Substrate (20 nM; EpiCypher 16-4201) incubated with SMARCA4 Remodeling Enzyme (EpiCypher 15-1014; concentrations indicated). Curves denote FRET efficiency and chromatin remodeling. Download Technical Datasheet for additional QC Data.
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