SKU: 21-0014
{"AddThisServiceButtonMeta":"","add_this":[{"annotation":"","service":"facebook"},{"annotation":"","service":"email"},{"annotation":"","service":"print"},{"annotation":"","service":"twitter"},{"annotation":"","service":"linkedin"}],"add_to_wishlist_url":"/wishlist.php?action=add&product_id=876","availability":"","bulk_discount_rates":[],"can_purchase":true,"cart_url":"https://www.epicypher.com/cart.php","category":["Recombinant Proteins/Modifying Enzymes"],"custom_fields":[{"id":"913","name":"Pack Size","value":"500 µL"}],"customizations":[],"description":"<table width=\"100%\" border=\"0\" cellspacing=\"2\" cellpadding=\"2\">\n \n <tbody>\n \n <tr valign=\"top\">\n \n <td>\n \n \n \n <table width=\"95%\" border=\"0\" cellspacing=\"1\" cellpadding=\"1\">\n \n <tbody>\n \n <tr>\n \n <td>\n \n <table width=\"100%\" border=\"0\" cellspacing=\"0\" cellpadding=\"0\">\n \n <tbody>\n \n <tr></tr>\n \n </tbody>\n \n </table>\n \n </td>\n \n </tr>\n \n <tr>\n \n <td valign=\"top\"><span style=\"font-family: Arial; \" class=\"Apple-style-span\"><span style=\"font-size: 12pt; \" class=\"Apple-style-span\"><b><span class=\"Apple-style-span\" style=\"font-family: Arial; \"><span class=\"Apple-style-span\" style=\"font-size: 12pt; \"><b>5x SMARCA Remodeling Assay Buffer<br>\n </b></span></span> Description:</b> 5x concentrated reaction buffer for use with SMARCA Remodeling Enzymes (EpiCypher 15-1014 and 15-1015). Optimized for use with SMARCA Remodeling Enzyme, produced in SF9 cells. SMARCA is an ATP-dependent chromatin remodeling complex that regulates nucleosome spacing.</span></span>\n \n </td>\n \n </tr>\n \n <tr>\n \n <td valign=\"top\"><span style=\"font-family: Arial; \" class=\"Apple-style-span\"><span style=\"font-size: 12pt; \" class=\"Apple-style-span\"><b><span class=\"Apple-style-span\" style=\"font-family: Arial; \"><span class=\"Apple-style-span\" style=\"font-size: 12pt; \"><b>SMARCA Remodeling Enzymes</b></span></span> Formulation:</b> 5x SMARCA Remodeling Assay Buffer (100 mM Tris HCl, pH 7.5, 250 mM KCl, 15 mM MgCl2, 0.05% (w/v) BSA, 0.05% (v/v) Tween 20) is formulated for use with SMARCA Remodeling Enzyme.<br />\n \n <br />\n \n </span></span>\n \n </td>\n \n </tr>\n \n <tr>\n \n <td valign=\"top\"><span style=\"font-family: Arial; \" class=\"Apple-style-span\"><span style=\"font-size: 12pt; \" class=\"Apple-style-span\"><b>Storage and Stability:</b> For best results, store 5x SMARCA Remodeling Assay buffer at 4˚C and avoid freezing.<br />\n \n <br />\n \n </span></span>\n \n </td>\n \n </tr>\n \n <tr>\n \n <td valign=\"top\"><p><span style=\"font-family: Arial; \" class=\"Apple-style-span\"><span style=\"font-size: 12pt; \" class=\"Apple-style-span\"><b>Application Notes:</b> This product is formulated to perform remodeling reactions using SMARCA Remodeling Enzyme and EpiDyne<sup>®</sup>-FRET substrate (EpiCypher 16-4201) in a 10 μL reaction containing 20 nM SMARCA, 20 nM EpiDyne-FRET, and 2.5 mM rATP. Enzyme remodels to completion in <30 minutes.</span></span></p>\n <p><span style=\"font-family: Arial; \" class=\"Apple-style-span\"><span style=\"font-size: 12pt; \" class=\"Apple-style-span\"> For further information, please contact techsupport@epicypher.com or refer to the EpiDyne<sup>®</sup>- FRET Technote (available at www.epicypher.com/technotes).</span></span><span style=\"font-family: Arial; \" class=\"Apple-style-span\"><span style=\"font-size: 12pt; \" class=\"Apple-style-span\"><br />\n \n </span></span></p>\n \n </td>\n \n </tr>\n \n <tr>\n \n <td valign=\"top\"><p><span style=\"font-family: Arial; \" class=\"Apple-style-span\"><span style=\"font-size: 12pt; \" class=\"Apple-style-span\"><br />\n \n </span></span><br />\n \n </p>\n \n </td>\n \n </tr>\n \n <tr>\n \n <td valign=\"top\"><span style=\"font-family: Arial; \" class=\"Apple-style-span\"><span style=\"font-size: 12pt; \" class=\"Apple-style-span\">View technical datasheet for this product. <a href=\"https://www.epicypher.com/content/documents/tds/21-0014.pdf\" target=\"_blank\"><img src=\"https://cdn7.bigcommerce.com/s-y9o92/content/documents/tds/icon.png\" alt=\"21-0014 Datasheet\" width=\"30\" height=\"40\" /></a></span></span>\n \n </td>\n \n </tr>\n \n </tbody>\n \n </table>\n \n </td>\n \n <td>\n \n <table width=\"100%\" border=\"0\" cellspacing=\"3\" cellpadding=\"3\">\n \n <tbody>\n \n <tr>\n \n <td align=\"center\"><a href=\"https://www.epicypher.com/content/images/products/proteins/15_1014_ATP_dep_chromatin_remodeling.jpg\" target=\"_new\">\n \n <img src=\"https://cdn7.bigcommerce.com/s-y9o92/content/images/products/proteins/15_1014_ATP_dep_chromatin_remodeling.jpg\" alt=\"15-1014 ATP-dependent Chromatin Remodeling \" width=\"250\" height=\"183\" /></a>\n \n </td>\n \n </tr>\n \n <tr>\n \n <td align=\"left\" valign=\"top\">\n \n <p><span style=\"font-family: Arial; \" class=\"Apple-style-span\"><span style=\"font-size: 10pt; \" class=\"Apple-style-span\"><strong>ATP-dependent Chromatin Remodeling Assay:</strong> EpiDyne-FRET Chromatin Remodeling Substrate (20 nM; EpiCypher 16-4201) incubated with SMARCA4 Remodeling Enzyme (EpiCypher 15-1014; concentrations indicated). Curves denote FRET efficiency and chromatin remodeling. Download Technical Datasheet for additional QC Data.<br />\n \n <strong>(Click image to enlarge) </strong></span></span> </p></td>\n \n </tr>\n \n </tbody>\n \n </table>\n \n \n \n \n <table width=\"100%\" border=\"0\" cellspacing=\"3\" cellpadding=\"3\">\n \n <tbody>\n \n <tr>\n \n <td align=\"center\"></td>\n \n </tr>\n \n <tr>\n \n <td align=\"left\" valign=\"top\"></td>\n \n </tr>\n \n </tbody>\n \n </table>\n \n </td>\n \n </tr>\n \n </tbody>\n\n</table>\n\n\n\n\n</body>\n</html>\n","detail_messages":"","gift_wrapping_available":false,"gtin":null,"id":"876","images":[{"alt":"5x SMARCA Remodeling Assay Buffer","data":"https://cdn11.bigcommerce.com/s-y9o92/images/stencil/{:size}/products/876/941/5x-smarca-remodeling-assay-buffer__61629.1645734533.jpg?c=2"}],"main_image":{"alt":"5x SMARCA Remodeling Assay 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Pack Size: 500 µL
