
EpiDyne® Remodeling Assay Substrate DNA ST601-GATC1
{"url":"https://www.epicypher.com/products/nucleosomes/epidyne-chromatin-remodeling-substrates/epidyne-remodeling-assay-substrate-dna-st601-gatc1","add_this":[{"service":"facebook","annotation":""},{"service":"email","annotation":""},{"service":"print","annotation":""},{"service":"twitter","annotation":""},{"service":"linkedin","annotation":""}],"gtin":null,"options":[],"id":"374","bulk_discount_rates":[],"can_purchase":true,"meta_description":"EpiDyne Remodeling Assay Substrate DNA (Positive Control)","category":["Nucleosomes/EpiDyne® Chromatin Remodeling Substrates","Nucleosomes/Nucleosome Components and Subunits"],"AddThisServiceButtonMeta":"","main_image":{"data":"https://cdn11.bigcommerce.com/s-y9o92/images/stencil/{:size}/products/374/292/DNA__93069.1614637188.gif?c=2","alt":"EpiDyne® Remodeling Assay Substrate DNA ST601-GATC1"},"add_to_wishlist_url":"/wishlist.php?action=add&product_id=374","shipping":{"calculated":true},"num_reviews":0,"weight":"0.01 LBS","custom_fields":[{"id":"637","name":"Pack Size","value":"50 µg"}],"sku":"18-4101","description":"<div class=\"service_accordion product-droppdown\">\n <div class=\"container\">\n <div id=\"prodAccordion\">\n <div id=\"ProductDescription\" class=\"Block Panel current\">\n <h3 class=\"sub-title1\">Description</h3>\n <div\n class=\"ProductDescriptionContainer product-droppdown__section-description-specific\"\n >\n <p>\n EpiDyne<sup>®</sup> Remodeling Assay Substrate DNA ST601-GATC1 is a\n 217 base-pair double-stranded DNA fragment containing the 601\n nucleosome positioning sequence [1], which has high affinity for\n histone octamers and is useful for nucleosome assembly. The DNA also\n includes a 3’ acceptor sequence to accommodate the histone octamer\n subsequent to remodeling. This positive control has a DpnII\n restriction site within the 601 sequence. When paired with the\n negative control DNA (EpiCypher\n <a\n href=\"/products/nucleosomes/epidyne-chromatin-remodeling-substrates/epidyne-remodeling-assay-substrate-dna-st601-gatc0\"\n >18-4100</a\n >) these controls illustrate the migration range for the Restriction\n Enzyme Assay. See the\n <a\n href=\"https://www.epicypher.com/content/documents/technotes/EpiDyne_REA.pdf\"\n >EpiDyne Nucleosome Remodeling Assay Tech Note</a\n >\n (Restriction Enzyme Assay) for more information.\n </p>\n </div>\n </div>\n </div>\n <div id=\"prodAccordion\">\n <div id=\"ProductDescription\" class=\"Block Panel current\">\n <h3 class=\"sub-title1\">Validation Data</h3>\n <div\n class=\"ProductDescriptionContainer product-droppdown__section-description-specific\"\n >\n <section class=\"image-picker\">\n <div class=\"image-picker__left\">\n <div\n class=\"image-picker__main-content_active image-picker__main-content\"\n >\n <div class=\"image-picker__header-content\">\n <button class=\"image-picker__left-arrow\">\n <svg\n class=\"image-picker__svg-left\"\n width=\"24\"\n height=\"24\"\n viewBox=\"0 0 24 24\"\n >\n <path\n d=\"M16.67 0l2.83 2.829-9.339 9.175 9.339 9.167-2.83 2.829-12.17-11.996z\"\n />\n </svg>\n </button>\n <a\n href=\"/content/images/products/nucleosomes/18-4101-dna-gel-data.png\"\n target=\"_blank\"\n class=\"image-picker__main-image-link\"\n ><img\n alt=\"18-4101-dna-gel-data\"\n src=\"/content/images/products/nucleosomes/18-4101-dna-gel-data.png\"\n class=\"image-picker__main-image\"\n />\n <span class=\"image-picker__main-image-caption\"\n >(Click to enlarge)</span\n ></a\n >\n <button class=\"image-picker__right-arrow\">\n <svg\n class=\"image-picker__svg-right\"\n width=\"24\"\n height=\"24\"\n viewBox=\"0 0 24 24\"\n >\n <path\n d=\"M7.33 24l-2.83-2.829 9.339-9.175-9.339-9.167 2.83-2.829 12.17 11.996z\"\n />\n </svg>\n </button>\n </div>\n <p>\n <span class=\"image-picker__span-content\"\n ><strong>Figure 1: DNA gel data </strong><br />\n ST601-GATC1 DNA resolved via native PAGE gel and stained\n with ethidium bromide to visualize DNA.\n <strong>Lane 1:</strong> Free DNA (100 ng).\n <strong>Lane 2:</strong> Free DNA incubated with 2U DpnII\n for 1 hr at 37°C (100 ng). <strong>Lane 3:</strong> Free DNA\n incubated with 2U MfeI for 1 hr at 37°C (100 ng). Migration\n patterns of DNA molecular weight markers are indicated.\n </span>\n </p>\n </div>\n </div>\n <aside class=\"image-picker__right\">\n <div class=\"image-picker__gallery\">\n <img\n alt=\"18-4101-dna-gel-data\"\n src=\"/content/images/products/nucleosomes/18-4101-dna-gel-data.png\"\n width=\"200\"\n class=\"image-picker__side-image image-picker__side-image_active\"\n role=\"button\"\n />\n </div>\n </aside>\n </section>\n </div>\n </div>\n </div>\n <div id=\"prodAccordion\">\n <div id=\"ProductDescription\" class=\"Block Panel\">\n <h3 class=\"sub-title1\">Technical Information</h3>\n <div\n class=\"ProductDescriptionContainer product-droppdown__section-description\"\n >\n <div class=\"product-tech-info\">\n <div class=\"product-tech-info__line-item\">\n <div class=\"product-tech-info__line-item-left\">\n <b>Formulation</b>\n </div>\n <div class=\"product-tech-info__line-item-right\">\n 50 µg lyophilized ST601-GATC1 DNA.\n </div>\n </div>\n <div class=\"product-tech-info__line-item\">\n <div class=\"product-tech-info__line-item-left\">\n <b>Storage and Stability</b>\n </div>\n <div class=\"product-tech-info__line-item-right\">\n Stable for 2 years at -20°C from date of receipt. After\n resuspending, aliquots should be stored at -80°C.\n </div>\n </div>\n </div>\n </div>\n </div>\n </div>\n <div id=\"prodAccordion\">\n <div id=\"ProductDescription\" class=\"Block Panel\">\n <h3 class=\"sub-title1\">Application Notes</h3>\n <div\n class=\"ProductDescriptionContainer product-droppdown__section-description\"\n >\n <p>\n ST601-GATC1 DNA is useful as a positive control for restriction\n enzyme accessibility nucleosome remodeling assays using the\n Biotinylated EpiDyne Remodeling Assay Substrate, as a DpnII (GATC in\n red) restriction enzyme site are present within the 601 sequence.\n The naturally occurring MfeI restriction site (AATTGG in bold)\n remains present as well.\n </p>\n </div>\n </div>\n </div>\n <div id=\"prodAccordion\">\n <div id=\"ProductDescription\" class=\"Block Panel\">\n <h3 class=\"sub-title1\">References</h3>\n <div\n class=\"ProductDescriptionContainer product-droppdown__section-description\"\n >\n <strong>Background References:</strong>\n <br />\n [1] Lowary & Widom <em>J. Mol. Biol.</em> (1998). PMID:\n <a\n href=\"https://pubmed.ncbi.nlm.nih.gov/9514715/\"\n target=\"_blank\"\n title=\"New DNA sequence rules for high affinity binding to histone octamer and sequence-directed nucleosome positioning\"\n >9514715</a\n ><br />\n </div>\n </div>\n </div>\n <div id=\"prodAccordion\">\n <div id=\"ProductDescription\" class=\"Block Panel\">\n <h3 class=\"sub-title1\">Documents & Resources</h3>\n <div\n class=\"ProductDescriptionContainer product-droppdown__section-description\"\n >\n <div class=\"product-documents\">\n <a\n href=\"/content/documents/tds/18-4101.pdf\"\n target=\"_blank\"\n class=\"product-documents__link\"\n >\n <svg\n version=\"1.1\"\n id=\"Layer_1\"\n xmlns=\"http://www.w3.org/2000/svg\"\n xmlns:xlink=\"http://www.w3.org/1999/xlink\"\n x=\"0px\"\n y=\"0px\"\n viewBox=\"0 0 228 240\"\n style=\"enable-background: new 0 0 228 240\"\n xml:space=\"preserve\"\n class=\"product-documents__icon\"\n alt=\"16-0030 Datasheet\"\n >\n <g>\n <path\n class=\"product-documents__svg-pdf\"\n d=\"M191.92,68.77l-47.69-47.69c-1.33-1.33-3.12-2.08-5.01-2.08H45.09C41.17,19,38,22.17,38,26.09v184.36\n c0,3.92,3.17,7.09,7.09,7.09h141.82c3.92,0,7.09-3.17,7.09-7.09V73.8C194,71.92,193.25,70.1,191.92,68.77z M177.65,77.06h-41.7\n v-41.7L177.65,77.06z M178.05,201.59H53.95V34.95h66.92v47.86c0,5.14,4.17,9.31,9.31,9.31h47.86V201.59z\"\n />\n </g>\n <rect\n x=\"20\"\n y=\"112\"\n class=\"product-documents__svg-background\"\n width=\"146\"\n height=\"76\"\n />\n <g>\n <path\n class=\"product-documents__svg-pdf\"\n d=\"M23.83,125.68h22.36c5.29,0,9.41,1.33,12.35,4c2.94,2.67,4.42,6.39,4.42,11.18c0,4.78-1.47,8.51-4.42,11.18\n c-2.94,2.67-7.06,4-12.35,4H34.59v18.29H23.83V125.68z M44.81,147.9c5.38,0,8.07-2.32,8.07-6.97c0-2.39-0.67-4.16-2-5.31\n c-1.33-1.15-3.36-1.73-6.07-1.73H34.59v14.01H44.81z\"\n />\n <path\n class=\"product-documents__svg-pdf\"\n d=\"M69.92,125.68h18.91c5.29,0,9.84,0.97,13.66,2.9c3.82,1.93,6.74,4.72,8.76,8.35\n c2.02,3.63,3.04,7.98,3.04,13.04c0,5.06-1,9.42-3,13.08c-2,3.66-4.91,6.45-8.73,8.38c-3.82,1.93-8.4,2.9-13.73,2.9H69.92V125.68z\n M88.07,165.63c10.35,0,15.52-5.22,15.52-15.66c0-10.4-5.17-15.59-15.52-15.59h-7.38v31.26H88.07z\"\n />\n <path\n class=\"product-documents__svg-pdf\"\n d=\"M122.57,125.68h32.84v8.49h-22.22v11.18h20.84v8.49h-20.84v20.49h-10.63V125.68z\"\n />\n </g>\n </svg>\n <span class=\"product-documents__info\">Technical Datasheet</span>\n </a>\n </div>\n </div>\n </div>\n </div>\n </div>\n</div>\n","tags":[],"warranty":"","price":{"without_tax":{"formatted":"$115.00","value":115,"currency":"USD"},"tax_label":"Sales Tax"},"detail_messages":"","availability":"","page_title":"EpiDyne Remodeling Assay Substrate DNA (Positive Control)","cart_url":"https://www.epicypher.com/cart.php","max_purchase_quantity":0,"mpn":null,"upc":null,"shipping_messages":[],"rating":0,"meta_keywords":"ATPase, EpiDyne Nucleosome Remodeling Assay Substrate ST601-GATC1, EpiDyne, chromatin remodeling, chromatin remodeler, swi.snf, swi, snf, chrac, rsc, SMARCA, BRM1, BRG1, 16-4101, nucleosome remodeling, nucleosome sliding, sliding, restriction enzyme assay, DAM methyltransferase assay","show_quantity_input":1,"title":"EpiDyne® Remodeling Assay Substrate DNA ST601-GATC1","gift_wrapping_available":false,"min_purchase_quantity":0,"customizations":[],"images":[{"data":"https://cdn11.bigcommerce.com/s-y9o92/images/stencil/{:size}/products/374/292/DNA__93069.1614637188.gif?c=2","alt":"EpiDyne® Remodeling Assay Substrate DNA ST601-GATC1"}]} Pack Size: 50 µg
Description
EpiDyne® Remodeling Assay Substrate DNA ST601-GATC1 is a 217 base-pair double-stranded DNA fragment containing the 601 nucleosome positioning sequence [1], which has high affinity for histone octamers and is useful for nucleosome assembly. The DNA also includes a 3’ acceptor sequence to accommodate the histone octamer subsequent to remodeling. This positive control has a DpnII restriction site within the 601 sequence. When paired with the negative control DNA (EpiCypher 18-4100) these controls illustrate the migration range for the Restriction Enzyme Assay. See the EpiDyne Nucleosome Remodeling Assay Tech Note (Restriction Enzyme Assay) for more information.
Validation Data
Figure 1: DNA gel data
ST601-GATC1 DNA resolved via native PAGE gel and stained
with ethidium bromide to visualize DNA.
Lane 1: Free DNA (100 ng).
Lane 2: Free DNA incubated with 2U DpnII
for 1 hr at 37°C (100 ng). Lane 3: Free DNA
incubated with 2U MfeI for 1 hr at 37°C (100 ng). Migration
patterns of DNA molecular weight markers are indicated.
Technical Information
Application Notes
ST601-GATC1 DNA is useful as a positive control for restriction enzyme accessibility nucleosome remodeling assays using the Biotinylated EpiDyne Remodeling Assay Substrate, as a DpnII (GATC in red) restriction enzyme site are present within the 601 sequence. The naturally occurring MfeI restriction site (AATTGG in bold) remains present as well.