EpiDyne® Remodeling Assay Substrate DNA ST601-GATC1

SKU: 18-4101
Pack Size: 50 µg


EpiDyne® Remodeling Assay Substrate DNA ST601-GATC1 is a 217 base-pair double-stranded DNA fragment containing the 601 nucleosome positioning sequence [1], which has high affinity for histone octamers and is useful for nucleosome assembly. The DNA also includes a 3’ acceptor sequence to accommodate the histone octamer subsequent to remodeling. This positive control has a DpnII restriction site within the 601 sequence. When paired with the negative control DNA (EpiCypher 18-4100) these controls illustrate the migration range for the Restriction Enzyme Assay. See the EpiDyne Nucleosome Remodeling Assay Tech Note (Restriction Enzyme Assay) for more information.

Validation Data

Figure 1: DNA gel data
ST601-GATC1 DNA resolved via native PAGE gel and stained with ethidium bromide to visualize DNA. Lane 1: Free DNA (100 ng). Lane 2: Free DNA incubated with 2U DpnII for 1 hr at 37°C (100 ng). Lane 3: Free DNA incubated with 2U MfeI for 1 hr at 37°C (100 ng). Migration patterns of DNA molecular weight markers are indicated.

Technical Information

50 µg lyophilized ST601-GATC1 DNA.
Storage and Stability
Stable for 2 years at -20°C from date of receipt. After resuspending, aliquots should be stored at -80°C.

Application Notes

ST601-GATC1 DNA is useful as a positive control for restriction enzyme accessibility nucleosome remodeling assays using the Biotinylated EpiDyne Remodeling Assay Substrate, as a DpnII (GATC in red) restriction enzyme site are present within the 601 sequence. The naturally occurring MfeI restriction site (AATTGG in bold) remains present as well.


Background References:
[1] Lowary & Widom J. Mol. Biol. (1998). PMID: 9514715

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