SKU: 15-1015
{"AddThisServiceButtonMeta":"","add_this":[{"annotation":"","service":"facebook"},{"annotation":"","service":"email"},{"annotation":"","service":"print"},{"annotation":"","service":"twitter"},{"annotation":"","service":"linkedin"}],"add_to_wishlist_url":"/wishlist.php?action=add&product_id=650","availability":"","bulk_discount_rates":[],"can_purchase":true,"cart_url":"https://www.epicypher.com/cart.php","category":["Nucleosomes/EpiDyne® Chromatin Remodeling Substrates/EpiDyne<sup>®</sup> Modifying Enzymes ","Recombinant Proteins/EpiDyne<sup>®</sup> Chromatin Remodeling Enzymes","Recombinant Proteins/Modifying Enzymes"],"custom_fields":[{"id":"440","name":"Pack size","value":"100 reactions"}],"customizations":[],"description":"<table width=\"100%\" border=\"0\" cellspacing=\"2\" cellpadding=\"2\">\n \n <tbody>\n \n <tr valign=\"top\">\n \n <td>\n \n \n \n <table width=\"95%\" border=\"0\" cellspacing=\"1\" cellpadding=\"1\">\n \n <tbody>\n \n <tr>\n \n <td>\n \n <table width=\"100%\" border=\"0\" cellspacing=\"0\" cellpadding=\"0\">\n \n <tbody>\n \n <tr>\n \n <td align=\"left\" valign=\"top\"><span style=\"font-family: Arial; \" class=\"Apple-style-span\"><span style=\"font-size: 10pt; \" class=\"Apple-style-span\"><b>Type: </b>Remodeler/ATPase</span></span>\n \n </td>\n \n <td width=\"196\" align=\"left\" valign=\"top\"><span style=\"font-family: Arial; \" class=\"Apple-style-span\"><span style=\"font-size: 10pt; \" class=\"Apple-style-span\"><b>Host: </b>SF9 cells</span></span>\n \n </td>\n \n </tr>\n \n <tr>\n \n <td align=\"left\" valign=\"top\"><span style=\"font-family: Arial; \" class=\"Apple-style-span\"><span style=\"font-size: 10pt; \" class=\"Apple-style-span\"><b>Mol Wgt.: </b>173.6 kDa</span></span>\n \n </td>\n \n <td align=\"left\" valign=\"top\"><span style=\"font-family: Arial; \" class=\"Apple-style-span\"><span style=\"font-size: 10pt; \" class=\"Apple-style-span\"><b>Epitope Tag: </b>FLAG</span></span>\n \n </td>\n \n </tr>\n \n </tbody>\n \n </table>\n \n </td>\n \n </tr>\n \n <tr>\n \n <td valign=\"top\"><p><span style=\"font-family: Arial; \" class=\"Apple-style-span\"><span style=\"font-size: 12pt; \" class=\"Apple-style-span\"><br />\n \n <b><span class=\"Apple-style-span\" style=\"font-family: Arial; \"><span class=\"Apple-style-span\" style=\"font-size: 12pt; \"><b>SMARCA2 Remodeling Enzyme </b></span></span> Description:</b> Full length recombinant human SMARCA2 (BRM) Remodeling Enzyme produced in SF9 cells. SMARCA2 is a crucial component of the SWI/SNF complex. It is an ATPdependent chromatin remodeling enzyme that regulates nucleosome spacing.</span></span></p>\n <p><strong>This is product includes 5x SMARCA2 Remodeling Assay Buffer </strong></p>\n <p><span style=\"font-family: Arial; \" class=\"Apple-style-span\"><span style=\"font-size: 12pt; \" class=\"Apple-style-span\"><br />\n \n <br />\n \n </span></span></p>\n \n </td>\n \n </tr>\n \n <tr>\n \n <td valign=\"top\"><span style=\"font-family: Arial; \" class=\"Apple-style-span\"><span style=\"font-size: 12pt; \" class=\"Apple-style-span\"><b><span class=\"Apple-style-span\" style=\"font-family: Arial; \"><span class=\"Apple-style-span\" style=\"font-size: 12pt; \"><b>SMARCA2 Remodeling Enzyme </b></span></span>Formulation:</b> SMARCA2 at 0.97 mg/ml in 3.6 μl of 20 mM Tris HCl pH 7.9, 150 mM NaCl, 2 mM MgCl2, 0.2 mM EDTA, 10 mM beta-glycerophosphate, 0.01% NP-40, 0.2 mM PMSF, 0.5 mM benzamidine, 1 mM DTT, 15% glycerol, 2 μg/ml leupeptin, 1 μg/ml aprotinin, 0.4 mg/ml recombinant \nhuman insulin, 0.4 mg/ml 3x FLAG peptide. Molarity: 5.6 μM<br />\n \n <br />\n \n </span></span>\n \n </td>\n \n </tr>\n \n <tr>\n \n <td valign=\"top\"><span style=\"font-family: Arial; \" class=\"Apple-style-span\"><span style=\"font-size: 12pt; \" class=\"Apple-style-span\"><b>Storage and Stability:</b> Stable for six months at -80°C from date of receipt. For best results, aliquot and avoid multiple freeze/thaws.<br />\n \n <br />\n \n </span></span>\n \n </td>\n \n </tr>\n \n <tr>\n \n <td valign=\"top\"><span style=\"font-family: Arial; \" class=\"Apple-style-span\"><span style=\"font-size: 12pt; \" class=\"Apple-style-span\"><b>Application Notes:</b> This product is sufficient to perform 100 remodeling\nreactions using EpiDyne®-FRET substrate (Catalog No. 16\n-4201). A single reaction is defined as 10 μl containing [20\nnM SMARCA2/ 20 nM EpiDyne®-FRET/ 2.5 mM rATP] and\nremodels to completion in <20 minutes. 5x SMARCA\nRemodeling Assay Buffer is included (100 mM Tris HCl, pH\n7.5, 250 mM KCl, 15 mM MgCl2, 0.05% (w/v) BSA, 0.05%\n(v/v) Tween 20). For best results, make single-use aliquots\nwhen first thawed.<br />\n \n </span></span>\n \n </td>\n \n </tr>\n \n <tr>\n \n <td valign=\"top\">\n \n <p><span style=\"font-family: Arial; \" class=\"Apple-style-span\"><span style=\"font-size: 12pt; \" class=\"Apple-style-span\"><b>References:</b><br />\n <br />\n \n </span></span><br />\n \n </p>\n \n </td>\n \n </tr>\n \n <tr>\n \n <td valign=\"top\"><span style=\"font-family: Arial; \" class=\"Apple-style-span\"><span style=\"font-size: 12pt; \" class=\"Apple-style-span\">View technical datasheet for this product. <a href=\"https://www.epicypher.com/content/documents/tds/15-1015.pdf\" target=\"_blank\"><img src=\"https://cdn7.bigcommerce.com/s-y9o92/content/documents/tds/icon.png\" alt=\"15-1015 Datasheet\" width=\"30\" height=\"40\" /></a></span></span>\n \n </td>\n \n </tr>\n \n </tbody>\n \n </table>\n \n </td>\n \n <td>\n \n <table width=\"100%\" border=\"0\" cellspacing=\"3\" cellpadding=\"3\">\n \n <tbody>\n \n <tr>\n \n <td align=\"center\"><a href=\"#\" target=\"_new\">\n \n <img src=\"https://cdn7.bigcommerce.com/s-y9o92/content/images/products/proteins/15_1015_ATP_dep_chromatin_remodeling.png\" alt=\"15-1015 ATP-dependent Chromatin Remodeling \" width=\"250\" height=\"183\" /></a>\n \n </td>\n \n </tr>\n \n <tr>\n \n <td align=\"left\" valign=\"top\">\n \n <p><span style=\"font-family: Arial; \" class=\"Apple-style-span\"><span style=\"font-size: 10pt; \" class=\"Apple-style-span\"><strong>ATP-dependent Chromatin Remodeling Assay:</strong> EpiDyne®-FRET Chromatin Remodeling Substrate (20 nM; Catalog No. 16-4201) incubated with SMARCA2 Remodeling Enzyme Complex (concentrations indicated). Curves denote FRET efficiency/chromatin remodeling.<br />\n \n <strong>(Click image to enlarge) </strong></span></span> </p></td>\n \n </tr>\n \n </tbody>\n \n </table>\n \n \n \n \n <table width=\"100%\" border=\"0\" cellspacing=\"3\" cellpadding=\"3\">\n \n <tbody>\n \n <tr>\n \n <td align=\"center\"><a href=\"#\" target=\"_new\">\n \n <img src=\"https://cdn7.bigcommerce.com/s-y9o92/content/images/products/proteins/15-1015-protein-gel.png\" alt=\"15-1015 Protein Gel Data\" width=\"145\" height=\"250\" /></a>\n \n </td>\n \n </tr>\n \n <tr>\n \n <td align=\"left\" valign=\"top\">\n \n <p><span style=\"font-family: Arial; \" class=\"Apple-style-span\"><span style=\"font-size: 10pt; \" class=\"Apple-style-span\"><span class=\"Apple-style-span\" style=\"font-size: 10pt; \"><strong>Protein Gel Data:</strong></span> SMARCA2 Remodeling Enzyme (1μg) was run on an SDS-PAGE gel and stained with\nCoomassie blue. The migration and moleuclar weight of the protein standards are indicated.<br />\n \n <strong>(Click image to enlarge) </strong></span></span></p>\n \n <p></p>\n \n </td>\n \n </tr>\n \n </tbody>\n \n </table>\n \n </td>\n \n </tr>\n \n </tbody>\n\n</table>\n\n\n\n\n</body>\n</html>\n","detail_messages":"","gift_wrapping_available":false,"id":"650","images":[{"alt":"SMARCA2 Chromatin Remodeling Enzyme (Human BRM)","data":"https://cdn11.bigcommerce.com/s-y9o92/images/stencil/{:size}/products/650/615/Untitled__98861.1556128740.png?c=2"}],"main_image":{"alt":"SMARCA2 Chromatin Remodeling Enzyme (Human BRM)","data":"https://cdn11.bigcommerce.com/s-y9o92/images/stencil/{:size}/products/650/615/Untitled__98861.1556128740.png?c=2"},"max_purchase_quantity":0,"meta_description":"BRM, Human BRM (SMARCA2) Chromatin Remodeling Enzyme, SMARCA2 Remodeling Enzyme Complex, SMARCA2, Remodeling Enzyme Complex, chromatin remodeling complex, EpiDyne control, EpiDyne, ","meta_keywords":"BRM, Human BRM (SMARCA2) Chromatin Remodeling Enzyme, SMARCA2 Remodeling Enzyme Complex, SMARCA2, Remodeling Enzyme Complex, chromatin remodeling complex, EpiDyne control, EpiDyne, ","min_purchase_quantity":0,"num_reviews":0,"options":[],"page_title":"SMARCA2 Chromatin Remodeling Enzyme (Human BRM)","price":{"tax_label":"Sales Tax","without_tax":{"currency":"USD","formatted":"$505.00","value":505}},"rating":0,"related_products":[{"add_to_cart_url":"https://www.epicypher.com/cart.php?action=add&product_id=651","add_to_wishlist_url":"/wishlist.php?action=add&product_id=651","availability":"","brand":{"name":null},"category":["Nucleosomes/EpiDyne® Chromatin Remodeling Substrates/EpiDyne<sup>®</sup> Modifying Enzymes ","Recombinant Proteins/EpiDyne<sup>®</sup> Chromatin Remodeling Enzymes","Recombinant Proteins/Modifying Enzymes"],"custom_fields":[{"id":441,"name":"Pack size","value":"100 reactions"}],"date_added":"15th Feb 2019","demo":false,"has_options":false,"id":651,"image":{"alt":"SMARCA4 Chromatin 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Pack size: 100 reactions
