| Type: Remodeler/ATPase
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Host: SF9 cells
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| Mol Wgt.: 173.6 kDa
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Epitope Tag: FLAG
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SMARCA2 Remodeling Enzyme Description: Full length recombinant human SMARCA2 (BRM) Remodeling Enzyme produced in SF9 cells. SMARCA2 is a crucial component of the SWI/SNF complex. It is an ATPdependent chromatin remodeling enzyme that regulates nucleosome spacing.
This is product includes 5x SMARCA2 Remodeling Assay Buffer
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SMARCA2 Remodeling Enzyme Formulation: SMARCA2 at 0.97 mg/ml in 3.6 μl of 20 mM Tris HCl pH 7.9, 150 mM NaCl, 2 mM MgCl2, 0.2 mM EDTA, 10 mM beta-glycerophosphate, 0.01% NP-40, 0.2 mM PMSF, 0.5 mM benzamidine, 1 mM DTT, 15% glycerol, 2 μg/ml leupeptin, 1 μg/ml aprotinin, 0.4 mg/ml recombinant
human insulin, 0.4 mg/ml 3x FLAG peptide. Molarity: 5.6 μM
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Storage and Stability: Stable for six months at -80°C from date of receipt. For best results, aliquot and avoid multiple freeze/thaws.
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Application Notes: This product is sufficient to perform 100 remodeling
reactions using EpiDyne®-FRET substrate (Catalog No. 16
-4201). A single reaction is defined as 10 μl containing [20
nM SMARCA2/ 20 nM EpiDyne®-FRET/ 2.5 mM rATP] and
remodels to completion in <20 minutes. 5x SMARCA
Remodeling Assay Buffer is included (100 mM Tris HCl, pH
7.5, 250 mM KCl, 15 mM MgCl2, 0.05% (w/v) BSA, 0.05%
(v/v) Tween 20). For best results, make single-use aliquots
when first thawed.
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References:
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View technical datasheet for this product. 
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ATP-dependent Chromatin Remodeling Assay: EpiDyne®-FRET Chromatin Remodeling Substrate (20 nM; Catalog No. 16-4201) incubated with SMARCA2 Remodeling Enzyme Complex (concentrations indicated). Curves denote FRET efficiency/chromatin remodeling.
(Click image to enlarge) |
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Protein Gel Data: SMARCA2 Remodeling Enzyme (1μg) was run on an SDS-PAGE gel and stained with
Coomassie blue. The migration and moleuclar weight of the protein standards are indicated.
(Click image to enlarge)
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