SMARCA2

SMARCA2 Chromatin Remodeling Enzyme (Human BRM)

$550.00
SKU: 15-1015
Pack Size: 100 Reactions
  • Type:  Remodeler/ATPase
  • Mol Wgt:  181 kDa
  • Expressed In:  SF9 cells
  • Epitope Tag:  FLAG

Description

Enzymatically active, full length recombinant human SMARCA2 Remodeling Enzyme (BRM, UniProt reference no. P51531-2) produced in SF9 cells. SMARCA2 is the catalytic subunit of the SWI/SNF complex. It is an ATP-dependent chromatin remodeling enzyme that regulates nucleosome spacing.

Validation Data

Figure 1: ATP-dependent chromatin remodeling assay
EpiDyne®-FRET Chromatin Remodeling Substrate (EpiCypher 16-4201; 15nM) incubated with SMARCA2 Remodeling Enzyme (concentrations indicated) in 1x SMARCA2 Remodeling Assay Buffer. Curves denote FRET efficiency/chromatin remodeling.

Figure 2: Protein gel data
SMARCA2 Remodeling Enzyme (1 µg) was run on an SDS-PAGE gel and stained with Coomassie blue. The migration and molecular weight of the protein standards are indicated.

Technical Information

Formulation:
SMARCA2 in 25 mM HEPES pH 7.6, 300 mM NaCl, 0.1 mM EDTA, 10% glycerol, 1 mM DTT, 0.4 mM PMSF, 0.1 mM benzamidine, 0.4 mg/mL recombinant human insulin.
Storage and Stability:
Stable for six months -80°C from date of receipt. For best results, aliquot and avoid multiple freeze/thaws.

Application Notes

This product is sufficient to perform 100 remodeling reactions using EpiDyne®-FRET substrate (EpiCypher 16-4201). A single reaction is defined as 10 µL containing [20 nM SMARCA2, 20 nM EpiDyne®-FRET, 1 mM rATP] and remodels to completion in <30 minutes. 5x SMARCA Remodeling Assay Buffer is included (EpiCypher 21-0014). For more information, see the EpiDyne®-FRET Technote or contact techsupport@epicypher.com.

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