

SMARCA2 Chromatin Remodeling Enzyme (Human BRM)
{"AddThisServiceButtonMeta":"","add_this":[{"annotation":"","service":"facebook"},{"annotation":"","service":"email"},{"annotation":"","service":"print"},{"annotation":"","service":"twitter"},{"annotation":"","service":"linkedin"}],"add_to_wishlist_url":"/wishlist.php?action=add&product_id=650","availability":"","bulk_discount_rates":[],"can_purchase":true,"cart_url":"https://www.epicypher.com/cart.php","category":["Nucleosomes/EpiDyne® Chromatin Remodeling Substrates/EpiDyne<sup>®</sup> Modifying Enzymes ","Recombinant Proteins/EpiDyne<sup>®</sup> Chromatin Remodeling Enzymes","Recombinant Proteins/Modifying Enzymes"],"custom_fields":[{"id":"1081","name":"Pack Size","value":"100 Reactions"},{"id":"1082","name":"Internal Comment","value":"extra in top right of Psylocke"}],"customizations":[],"description":"<div class=\"product-general-info\">\n <ul class=\"product-general-info__list-left\">\n <li class=\"product-general-info__list-item\">\n <strong>Type: </strong>Remodeler/ATPase\n </li>\n <li class=\"product-general-info__list-item\">\n <strong>Mol Wgt: </strong>181 kDa\n </li>\n </ul>\n <ul class=\"product-general-info__list-right\">\n <li class=\"product-general-info__list-item\">\n <strong>Expressed In: </strong>SF9 cells\n </li>\n <li class=\"product-general-info__list-item\">\n <strong>Epitope Tag: </strong>FLAG\n </li>\n </ul>\n</div>\n<div class=\"service_accordion product-droppdown\">\n <div class=\"container\">\n <div id=\"prodAccordion\">\n <div id=\"ProductDescription\" class=\"Block Panel current\">\n <h3 class=\"sub-title1\">Description</h3>\n <div\n class=\"ProductDescriptionContainer product-droppdown__section-description-specific\"\n style=\"display: block\"\n >\n <p>\n Enzymatically active, full length recombinant human SMARCA2\n Remodeling Enzyme (BRM, UniProt reference no. P51531-2) produced in\n SF9 cells. SMARCA2 is the catalytic subunit of the SWI/SNF complex.\n It is an ATP-dependent chromatin remodeling enzyme that regulates\n nucleosome spacing.\n </p>\n </div>\n </div>\n </div>\n <div id=\"prodAccordion\">\n <div id=\"ProductDescription\" class=\"Block Panel current\">\n <h3 class=\"sub-title1\">Validation Data</h3>\n <div\n class=\"ProductDescriptionContainer product-droppdown__section-description-specific\"\n style=\"display: block\"\n >\n <section class=\"image-picker\">\n <div class=\"image-picker__left\">\n <div\n class=\"image-picker__main-content_active image-picker__main-content\"\n >\n <div class=\"image-picker__header-content\">\n <button class=\"image-picker__left-arrow\">\n <svg\n class=\"image-picker__svg-left\"\n width=\"24\"\n height=\"24\"\n viewBox=\"0 0 24 24\"\n >\n <path\n d=\"M16.67 0l2.83 2.829-9.339 9.175 9.339 9.167-2.83 2.829-12.17-11.996z\"\n />\n </svg>\n </button>\n <a\n href=\"/content/images/products/proteins/15-1015-chromatin-remodeling-assay.jpeg\"\n target=\"blank\"\n class=\"image-picker__main-image-link\"\n ><img\n alt=\"15-1015-chromatin-remodeling-assay\"\n src=\"/content/images/products/proteins/15-1015-chromatin-remodeling-assay.jpeg\"\n class=\"image-picker__main-image\"\n />\n <span class=\"image-picker__main-image-caption\"\n >(Click to enlarge)</span\n ></a\n >\n <button class=\"image-picker__right-arrow\">\n <svg\n class=\"image-picker__svg-right\"\n width=\"24\"\n height=\"24\"\n viewBox=\"0 0 24 24\"\n >\n <path\n d=\"M7.33 24l-2.83-2.829 9.339-9.175-9.339-9.167 2.83-2.829 12.17 11.996z\"\n />\n </svg>\n </button>\n </div>\n <p>\n <span class=\"image-picker__span-content\"\n ><strong\n >Figure 1: ATP-dependent chromatin remodeling assay </strong\n ><br />EpiDyne<sup>®</sup>-FRET Chromatin Remodeling\n Substrate (EpiCypher\n <a\n href=\"/products/nucleosomes/epidyne-fret-nucleosome-remodeling-assay-substrate\"\n >16-4201</a\n >; 15nM) incubated with SMARCA2 Remodeling Enzyme\n (concentrations indicated) in 1x SMARCA2 Remodeling Assay\n Buffer. Curves denote FRET efficiency/chromatin remodeling.\n </span>\n </p>\n </div>\n <div class=\"image-picker__main-content\">\n <div class=\"image-picker__header-content\">\n <button class=\"image-picker__left-arrow\">\n <svg\n class=\"image-picker__svg-left\"\n width=\"24\"\n height=\"24\"\n viewBox=\"0 0 24 24\"\n >\n <path\n d=\"M16.67 0l2.83 2.829-9.339 9.175 9.339 9.167-2.83 2.829-12.17-11.996z\"\n />\n </svg>\n </button>\n <a\n href=\"/content/images/products/proteins/15-1015-protein-gel-data.jpeg\"\n target=\"blank\"\n class=\"image-picker__main-image-link\"\n ><img\n alt=\"15-1015-protein-gel-data\"\n src=\"/content/images/products/proteins/15-1015-protein-gel-data.jpeg\"\n class=\"image-picker__main-image\"\n />\n <span class=\"image-picker__main-image-caption\"\n >(Click to enlarge)</span\n ></a\n >\n <button class=\"image-picker__right-arrow\">\n <svg\n class=\"image-picker__svg-right\"\n width=\"24\"\n height=\"24\"\n viewBox=\"0 0 24 24\"\n >\n <path\n d=\"M7.33 24l-2.83-2.829 9.339-9.175-9.339-9.167 2.83-2.829 12.17 11.996z\"\n />\n </svg>\n </button>\n </div>\n <p>\n <span class=\"image-picker__span-content\"\n ><strong>Figure 2: Protein gel data </strong><br />\n SMARCA2 Remodeling Enzyme (1 µg) was run on an SDS-PAGE gel\n and stained with Coomassie blue. The migration and molecular\n weight of the protein standards are indicated.\n </span>\n </p>\n </div>\n </div>\n <aside class=\"image-picker__right\">\n <div class=\"image-picker__gallery\">\n <img\n alt=\"15-1015-chromatin-remodeling-assay\"\n src=\"/content/images/products/proteins/15-1015-chromatin-remodeling-assay.jpeg\"\n width=\"200\"\n class=\"image-picker__side-image image-picker__side-image_active\"\n role=\"button\"\n />\n <img\n alt=\"15-1015-protein-gel-data\"\n src=\"/content/images/products/proteins/15-1015-protein-gel-data.jpeg\"\n class=\"image-picker__side-image\"\n role=\"button\"\n />\n </div>\n </aside>\n </section>\n </div>\n </div>\n </div>\n <div id=\"prodAccordion\">\n <div id=\"ProductDescription\" class=\"Block Panel\">\n <h3 class=\"sub-title1\">Technical Information</h3>\n <div\n class=\"ProductDescriptionContainer product-droppdown__section-description\"\n >\n <div class=\"product-tech-info\">\n <div class=\"product-tech-info__line-item\">\n <div class=\"product-tech-info__line-item-left\">\n <b>Formulation:</b>\n </div>\n <div class=\"product-tech-info__line-item-right\">\n SMARCA2 in 25 mM HEPES pH 7.6, 300 mM NaCl, 0.1 mM EDTA, 10%\n glycerol, 1 mM DTT, 0.4 mM PMSF, 0.1 mM benzamidine, 0.4 mg/mL\n recombinant human insulin.\n </div>\n </div>\n <div class=\"product-tech-info__line-item\">\n <div class=\"product-tech-info__line-item-left\">\n <b>Storage and Stability:</b>\n </div>\n <div class=\"product-tech-info__line-item-right\">\n Stable for six months -80°C from date of receipt. For best\n results, aliquot and avoid multiple freeze/thaws.\n </div>\n </div>\n </div>\n </div>\n </div>\n </div>\n <div id=\"prodAccordion\">\n <div id=\"ProductDescription\" class=\"Block Panel\">\n <h3 class=\"sub-title1\">Application Notes</h3>\n <div\n class=\"ProductDescriptionContainer product-droppdown__section-description\"\n >\n <p>\n This product is sufficient to perform 100 remodeling reactions using\n EpiDyne<sup>®</sup>-FRET substrate (EpiCypher\n <a\n href=\"/products/nucleosomes/epidyne-fret-nucleosome-remodeling-assay-substrate\"\n >16-4201</a\n >). A single reaction is defined as 10 µL containing [20 nM SMARCA2,\n 20 nM EpiDyne<sup>®</sup>-FRET, 1 mM rATP] and remodels to\n completion in <30 minutes. 5x SMARCA Remodeling Assay Buffer is\n included (EpiCypher\n <a\n href=\"/products/recombinant-proteins/modifying-enzymes/5x-smarca-remodeling-assay-buffer\"\n >21-0014</a\n >). For more information, see the\n <a href=\"/resources/technical-notes/\"\n >EpiDyne<sup>®</sup>-FRET Technote</a\n >\n or contact\n <a href=\"mailto:techsupport@epicypher.com\"\n >techsupport@epicypher.com</a\n >.\n </p>\n </div>\n </div>\n </div>\n\n <div id=\"prodAccordion\">\n <div id=\"ProductDescription\" class=\"Block Panel\">\n <h3 class=\"sub-title1\">Documents & Resources</h3>\n <div\n class=\"ProductDescriptionContainer product-droppdown__section-description\"\n >\n <div class=\"product-documents\">\n <a\n href=\"/content/documents/tds/15-1015.pdf\"\n target=\"blank\"\n class=\"product-documents__link\"\n >\n <svg\n version=\"1.1\"\n id=\"Layer_1\"\n xmlns=\"http://www.w3.org/2000/svg\"\n xmlns:xlink=\"http://www.w3.org/1999/xlink\"\n x=\"0px\"\n y=\"0px\"\n viewBox=\"0 0 228 240\"\n style=\"enable-background: new 0 0 228 240\"\n xml:space=\"preserve\"\n class=\"product-documents__icon\"\n >\n <g>\n <path\n class=\"product-documents__svg-pdf\"\n 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c2.02,3.63,3.04,7.98,3.04,13.04c0,5.06-1,9.42-3,13.08c-2,3.66-4.91,6.45-8.73,8.38c-3.82,1.93-8.4,2.9-13.73,2.9H69.92V125.68z\n M88.07,165.63c10.35,0,15.52-5.22,15.52-15.66c0-10.4-5.17-15.59-15.52-15.59h-7.38v31.26H88.07z\"\n />\n <path\n class=\"product-documents__svg-pdf\"\n d=\"M122.57,125.68h32.84v8.49h-22.22v11.18h20.84v8.49h-20.84v20.49h-10.63V125.68z\"\n />\n </g>\n </svg>\n <span class=\"product-documents__info\">Technical Datasheet</span>\n </a>\n </div>\n </div>\n </div>\n </div>\n </div>\n</div>\n","detail_messages":"","gift_wrapping_available":false,"gtin":null,"id":"650","images":[{"alt":"SMARCA2 Chromatin Remodeling Enzyme Human BRM","data":"https://cdn11.bigcommerce.com/s-y9o92/images/stencil/{:size}/products/650/953/smarca2-chromatin-remodeling-enzyme-human-brm__84445.1645734545.jpg?c=2"}],"main_image":{"alt":"SMARCA2 Chromatin Remodeling Enzyme Human 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- Type: Remodeler/ATPase
- Mol Wgt: 181 kDa
- Expressed In: SF9 cells
- Epitope Tag: FLAG
Description
Enzymatically active, full length recombinant human SMARCA2 Remodeling Enzyme (BRM, UniProt reference no. P51531-2) produced in SF9 cells. SMARCA2 is the catalytic subunit of the SWI/SNF complex. It is an ATP-dependent chromatin remodeling enzyme that regulates nucleosome spacing.
Validation Data
Figure 1: ATP-dependent chromatin remodeling assay
EpiDyne®-FRET Chromatin Remodeling
Substrate (EpiCypher
16-4201; 15nM) incubated with SMARCA2 Remodeling Enzyme
(concentrations indicated) in 1x SMARCA2 Remodeling Assay
Buffer. Curves denote FRET efficiency/chromatin remodeling.
Figure 2: Protein gel data
SMARCA2 Remodeling Enzyme (1 µg) was run on an SDS-PAGE gel
and stained with Coomassie blue. The migration and molecular
weight of the protein standards are indicated.
Technical Information
Application Notes
This product is sufficient to perform 100 remodeling reactions using EpiDyne®-FRET substrate (EpiCypher 16-4201). A single reaction is defined as 10 µL containing [20 nM SMARCA2, 20 nM EpiDyne®-FRET, 1 mM rATP] and remodels to completion in <30 minutes. 5x SMARCA Remodeling Assay Buffer is included (EpiCypher 21-0014). For more information, see the EpiDyne®-FRET Technote or contact techsupport@epicypher.com.