SKU: 15-1015
{"AddThisServiceButtonMeta":"","add_this":[{"annotation":"","service":"facebook"},{"annotation":"","service":"email"},{"annotation":"","service":"print"},{"annotation":"","service":"twitter"},{"annotation":"","service":"linkedin"}],"add_to_wishlist_url":"/wishlist.php?action=add&product_id=650","availability":"","bulk_discount_rates":[],"can_purchase":false,"cart_url":"https://www.epicypher.com/cart.php","category":["Nucleosomes/EpiDyne® Chromatin Remodeling Substrates/EpiDyne<sup>®</sup> Modifying Enzymes ","Recombinant Proteins/EpiDyne<sup>®</sup> Chromatin Remodeling Enzymes","Recombinant Proteins/Modifying Enzymes"],"custom_fields":[{"id":"440","name":"Pack size","value":"100 reactions"}],"customizations":[],"description":"<table width=\"100%\" border=\"0\" cellspacing=\"2\" cellpadding=\"2\">\n \n <tbody>\n \n <tr valign=\"top\">\n \n <td>\n \n \n \n <table width=\"95%\" border=\"0\" cellspacing=\"1\" cellpadding=\"1\">\n \n <tbody>\n \n <tr>\n \n <td>\n \n <table width=\"100%\" border=\"0\" cellspacing=\"0\" cellpadding=\"0\">\n \n <tbody>\n \n <tr>\n \n <td align=\"left\" valign=\"top\"><span style=\"font-family: Arial; \" class=\"Apple-style-span\"><span style=\"font-size: 10pt; \" class=\"Apple-style-span\"><b>Type: </b>Remodeler/ATPase</span></span>\n \n </td>\n \n <td width=\"196\" align=\"left\" valign=\"top\"><span style=\"font-family: Arial; \" class=\"Apple-style-span\"><span style=\"font-size: 10pt; \" class=\"Apple-style-span\"><b>Host: </b>SF9 cells</span></span>\n \n </td>\n \n </tr>\n \n <tr>\n \n <td align=\"left\" valign=\"top\"><span style=\"font-family: Arial; \" class=\"Apple-style-span\"><span style=\"font-size: 10pt; \" class=\"Apple-style-span\"><b>Mol Wgt.: </b>173.6 kDa</span></span>\n \n </td>\n \n <td align=\"left\" valign=\"top\"><span style=\"font-family: Arial; \" class=\"Apple-style-span\"><span style=\"font-size: 10pt; \" class=\"Apple-style-span\"><b>Epitope Tag: </b>FLAG</span></span>\n \n </td>\n \n </tr>\n \n </tbody>\n \n </table>\n \n </td>\n \n </tr>\n \n <tr>\n \n <td valign=\"top\"><p><span style=\"font-family: Arial; \" class=\"Apple-style-span\"><span style=\"font-size: 12pt; \" class=\"Apple-style-span\"><br />\n \n <b><span class=\"Apple-style-span\" style=\"font-family: Arial; \"><span class=\"Apple-style-span\" style=\"font-size: 12pt; \"><b>SMARCA2 Remodeling Enzyme </b></span></span> Description:</b> Full length recombinant human SMARCA2 (BRM) Remodeling Enzyme produced in SF9 cells. SMARCA2 is a crucial component of the SWI/SNF complex. It is an ATPdependent chromatin remodeling enzyme that regulates nucleosome spacing.</span></span></p>\n <p><strong>This is product includes 5x SMARCA2 Remodeling Assay Buffer </strong></p>\n <p><span style=\"font-family: Arial; \" class=\"Apple-style-span\"><span style=\"font-size: 12pt; \" class=\"Apple-style-span\"><br />\n \n <br />\n \n </span></span></p>\n \n </td>\n \n </tr>\n \n <tr>\n \n <td valign=\"top\"><span style=\"font-family: Arial; \" class=\"Apple-style-span\"><span style=\"font-size: 12pt; \" class=\"Apple-style-span\"><b><span class=\"Apple-style-span\" style=\"font-family: Arial; \"><span class=\"Apple-style-span\" style=\"font-size: 12pt; \"><b>SMARCA2 Remodeling Enzyme </b></span></span>Formulation:</b> SMARCA2 supplied in 20 mM Tris HCl pH 7.9, 150 mM NaCl, 2 mM MgCl2, 0.2 mM EDTA, 10 mM beta-glycerophosphate, 0.01% NP-40, 0.2 mM PMSF, 0.5 mM benzamidine, 1 mM DTT, 15% glycerol, 2 μg/ml leupeptin, 1 μg/ml aprotinin, 0.4 mg/ml recombinant \nhuman insulin, 0.4 mg/ml 3x FLAG peptide. <br />\n \n <br />\n \n </span></span>\n \n </td>\n \n </tr>\n \n <tr>\n \n <td valign=\"top\"><span style=\"font-family: Arial; \" class=\"Apple-style-span\"><span style=\"font-size: 12pt; \" class=\"Apple-style-span\"><b>Storage and Stability:</b> Stable for six months at -80°C from date of receipt. For best results, aliquot and avoid multiple freeze/thaws.<br />\n \n <br />\n \n </span></span>\n \n </td>\n \n </tr>\n \n <tr>\n \n <td valign=\"top\"><span style=\"font-family: Arial; \" class=\"Apple-style-span\"><span style=\"font-size: 12pt; \" class=\"Apple-style-span\"><b>Application Notes:</b> This product is sufficient to perform 100 remodeling\nreactions using EpiDyne®-FRET substrate (Catalog No. 16-4201). The information stated is representative and may not reflect the specific lot received upon ordering. Due to lot specific activity variability, this product may differ in concentration or total mass across lots. Each lot is tested independently and enzyme sufficient for 100 reactions* is provided. Each lot is supplied with a lot specific technical datasheet (TDS). Please refer to the TDS for assay formulation and reaction conditions. 5x SMARCA\nRemodeling Assay Buffer is included (100 mM Tris HCl, pH\n7.5, 250 mM KCl, 15 mM MgCl2, 0.05% (w/v) BSA, 0.05%\n(v/v) Tween 20). For best results, make single-use aliquots\nwhen first thawed. <br>*A single reaction is defined as 10 µL containing [Lot specific concentration of remodeling enzyme/ 20 nM EpiDyne®-FRET/ 1 mM rATP ] and remodels to completion in 30 minutes.<br />\n \n </span></span>\n \n </td>\n \n </tr>\n \n <tr>\n \n <td valign=\"top\">\n \n <p><span style=\"font-family: Arial; \" class=\"Apple-style-span\"><span style=\"font-size: 12pt; \" class=\"Apple-style-span\"><b>References:</b><br />\n <br />\n \n </span></span><br />\n \n </p>\n \n </td>\n \n </tr>\n \n <tr>\n \n <td valign=\"top\"><span style=\"font-family: Arial; \" class=\"Apple-style-span\"><span style=\"font-size: 12pt; \" class=\"Apple-style-span\">View technical datasheet for this product. <a href=\"https://www.epicypher.com/content/documents/tds/15-1015.pdf\" target=\"_blank\"><img src=\"https://cdn7.bigcommerce.com/s-y9o92/content/documents/tds/icon.png\" alt=\"15-1015 Datasheet\" width=\"30\" height=\"40\" /></a></span></span>\n \n </td>\n \n </tr>\n \n </tbody>\n \n </table>\n \n </td>\n \n <td>\n \n <table width=\"100%\" border=\"0\" cellspacing=\"3\" cellpadding=\"3\">\n \n <tbody>\n \n <tr>\n \n <td align=\"center\"><a href=\"#\" target=\"_new\">\n \n <img src=\"https://cdn7.bigcommerce.com/s-y9o92/content/images/products/proteins/15_1015_ATP_dep_chromatin_remodeling.png\" alt=\"15-1015 ATP-dependent Chromatin Remodeling \" width=\"250\" height=\"183\" /></a>\n \n </td>\n \n </tr>\n \n <tr>\n \n <td align=\"left\" valign=\"top\">\n \n <p><span style=\"font-family: Arial; \" class=\"Apple-style-span\"><span style=\"font-size: 10pt; \" class=\"Apple-style-span\"><strong>ATP-dependent Chromatin Remodeling Assay:</strong> EpiDyne®-FRET Chromatin Remodeling Substrate (20 nM; Catalog No. 16-4201) incubated with SMARCA2 Remodeling Enzyme Complex (concentrations indicated). Curves denote FRET efficiency/chromatin remodeling.<br />\n \n <strong>(Click image to enlarge) </strong></span></span> </p></td>\n \n </tr>\n \n </tbody>\n \n </table>\n \n \n \n \n <table width=\"100%\" border=\"0\" cellspacing=\"3\" cellpadding=\"3\">\n \n <tbody>\n \n <tr>\n \n <td align=\"center\"><a href=\"#\" target=\"_new\">\n \n <img src=\"https://cdn7.bigcommerce.com/s-y9o92/content/images/products/proteins/15-1015-protein-gel.png\" alt=\"15-1015 Protein Gel Data\" width=\"145\" height=\"250\" /></a>\n \n </td>\n \n </tr>\n \n <tr>\n \n <td align=\"left\" valign=\"top\">\n \n <p><span style=\"font-family: Arial; \" class=\"Apple-style-span\"><span style=\"font-size: 10pt; \" class=\"Apple-style-span\"><span class=\"Apple-style-span\" style=\"font-size: 10pt; \"><strong>Protein Gel Data:</strong></span> SMARCA2 Remodeling Enzyme (1μg) was run on an SDS-PAGE gel and stained with\nCoomassie blue. The migration and moleuclar weight of the protein standards are indicated.<br />\n \n <strong>(Click image to enlarge) </strong></span></span></p>\n \n <p></p>\n \n </td>\n \n </tr>\n \n </tbody>\n \n </table>\n \n </td>\n \n </tr>\n \n </tbody>\n\n</table>\n\n\n\n\n</body>\n</html>\n","detail_messages":"","gift_wrapping_available":false,"gtin":null,"id":"650","images":[{"alt":"SMARCA2 Chromatin Remodeling Enzyme (Human BRM)","data":"https://cdn11.bigcommerce.com/s-y9o92/images/stencil/{:size}/products/650/615/Untitled__98861.1556128740.png?c=2"}],"main_image":{"alt":"SMARCA2 Chromatin Remodeling Enzyme (Human BRM)","data":"https://cdn11.bigcommerce.com/s-y9o92/images/stencil/{:size}/products/650/615/Untitled__98861.1556128740.png?c=2"},"max_purchase_quantity":0,"meta_description":"BRM, Human BRM (SMARCA2) Chromatin Remodeling Enzyme, SMARCA2 Remodeling Enzyme Complex, SMARCA2, Remodeling Enzyme Complex, chromatin remodeling complex, EpiDyne control, EpiDyne, ","meta_keywords":"BRM, Human BRM (SMARCA2) Chromatin Remodeling Enzyme, SMARCA2 Remodeling Enzyme Complex, SMARCA2, Remodeling Enzyme Complex, chromatin remodeling complex, EpiDyne control, EpiDyne, ","min_purchase_quantity":0,"mpn":null,"num_reviews":0,"options":[],"page_title":"SMARCA2 Chromatin Remodeling Enzyme (Human BRM)","price":{"tax_label":"Sales Tax","without_tax":{"currency":"USD","formatted":"$550.00","value":550}},"rating":0,"related_products":[{"add_to_cart_url":"https://www.epicypher.com/cart.php?action=add&product_id=651","add_to_wishlist_url":"/wishlist.php?action=add&product_id=651","availability":"","brand":{"name":null},"category":["Nucleosomes/EpiDyne® Chromatin Remodeling Substrates/EpiDyne<sup>®</sup> Modifying Enzymes ","Recombinant Proteins/EpiDyne<sup>®</sup> Chromatin Remodeling Enzymes","Recombinant Proteins/Modifying Enzymes"],"custom_fields":[{"id":756,"name":"Pack size","value":"100 reactions"},{"id":757,"name":"Internal Comment","value":"extra in bottom right of Psylocke, bulk 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Pack size: 100 reactions
