5x ACF Remodeling Assay Buffer Description: 5x concentrated reaction buffer for use with ACF Remodeling Enzyme Complex (Catalog No. 15-1013). Optimized for use with recombinant Drosophila ACF Remodeling Enzyme Complex, assembled from Acf1-FLAG and ISWI produced in SF9 cells. ACF is an ATP-dependent chromatin remodeling complex that regulates nucleosome spacing.
ACF Remodeling Enzyme Complex Formulation: 5x ACF Remodeling Assay Buffer (100 mM Tris HCl, pH 7.5, 250 mM KCl, 15 mM MgCl2, 0.05% (w/v) BSA, 0.05% (v/v) Tween 20) is formulated for use with ACF Remodeling Enzyme Complex.
Storage and Stability: Stable for six months at 4°C from date of receipt.
Application Notes: This produt is formulated to perform remodeling reactions using ACF Remodeling Enzyme Complex and EpiDyne®-FRET substrate in a reaction defined as 10 μl containing [5 nM ACF Complex/ 20 nM EpiDyne®-FRET/ 50 μM rATP] and remodels to completion in <20 minutes. For best results, store 5x ACF Remodeling Assay buffer at 4ºC and avoid freezing.
For further information, please contact email@example.com or refer to the EpiDyne®- FRET Technote (TN#106; available at www.epicypher.com/technotes).
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ATP-dependent Chromatin Remodeling Assay: EpiDyne®- FRET Chromatin Remodeling Substrate (20 nM; Catalog No. 16-4201) incubated with ACF Remodeling Enzyme Complex (Catalog No. 15-1013) using ACF Remodeling Assay Buffer with or without ATP (50 μM). Curves denote FRET efficiency/chromatin remodeling. (Click image to enlarge)