CUTANA™ DNA Purification Kit

$205.00

SKU: 14-0050
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High\n yield purification and concentration of partitioned chromatin DNA is\n an essential step - one that requires a workflow specifically\n compatible with low starting material. Utilizing tapered cleanup\n columns designed for low elution volumes (as low as 6 µL), this kit\n produces purified, concentrated DNA ready for library preparation\n and next-generation sequencing (<strong>Figure 1</strong>). The\n user-friendly workflow can be completed in just 30 minutes. The\n columns recover > 50 bp DNA fragments, making the DNA Purification\n Kit ideal for a variety of CUT&RUN targets, including histone PTMs\n and transcription factors.\n </p>\n </div>\n </div>\n </div>\n <div id=\"prodAccordion\">\n <div id=\"ProductDescription\" class=\"Block Panel current\">\n <h3 class=\"sub-title1\">Validation Data</h3>\n <div\n class=\"ProductDescriptionContainer product-droppdown__section-description-specific\"\n >\n <section class=\"image-picker\">\n <div class=\"image-picker__left\">\n <div\n class=\"image-picker__main-content_active image-picker__main-content\"\n >\n <div class=\"image-picker__header-content\">\n <button class=\"image-picker__left-arrow\">\n <svg\n class=\"image-picker__svg-left\"\n width=\"24\"\n height=\"24\"\n viewBox=\"0 0 24 24\"\n >\n <path\n d=\"M16.67 0l2.83 2.829-9.339 9.175 9.339 9.167-2.83 2.829-12.17-11.996z\"\n />\n </svg>\n </button>\n <a\n href=\"/content/images/products/kits/14-0050-dna-fragment-size-distribution-analysis.jpeg\"\n target=\"_new\"\n class=\"image-picker__main-image-link\"\n ><img\n alt=\"14-0050-dna-fragment-size-distribution-analysis\"\n src=\"/content/images/products/kits/14-0050-dna-fragment-size-distribution-analysis.jpeg\"\n class=\"image-picker__main-image\"\n />\n <span class=\"image-picker__main-image-caption\"\n >(Click to enlarge)</span\n ></a\n >\n <button class=\"image-picker__right-arrow\">\n <svg\n class=\"image-picker__svg-right\"\n width=\"24\"\n height=\"24\"\n viewBox=\"0 0 24 24\"\n >\n <path\n d=\"M7.33 24l-2.83-2.829 9.339-9.175-9.339-9.167 2.83-2.829 12.17 11.996z\"\n />\n </svg>\n </button>\n </div>\n <p>\n <span class=\"image-picker__span-content\"\n ><strong\n >Figure 1: CUT&RUN DNA fragment size distribution\n analysis</strong\n ><br />\n 500,000 K562 cells per sample were processed with the\n CUTANA™ ChIC/CUT&RUN Kit (EpiCypher\n <a\n href=\"/products/epigenetics-reagents-and-assays/cutana-chic-cut-and-run-kit\"\n >14-1048</a\n >) following treatment with IgG negative control (EpiCypher\n <a\n href=\"/products/nucleosomes/snap-cutana-spike-in-controls/cutana-rabbit-igg-cut-run-negative-control-antibody\"\n >13-0042</a\n >), H3K4me3 (EpiCypher\n <a\n href=\"/products/antibodies/snap-chip-certified-antibodies/histone-h3k4me3-antibody-snap-chip-certified-cutana-cut-run-compatible\"\n >13-0041</a\n >), H3K27me3 (EpiCypher\n <a\n href=\"/products/antibodies/snap-chip-certified-antibodies/histone-h3k27me3-antibody-snap-chip-certified-cutana-cut-run-compatible-discontinued\"\n >13-0030</a\n >) and CTCF (EMD Millipore Catalog No. 07-729) antibodies.\n Isolated DNA was used to prepare paired-end Illumina<sup\n >®</sup\n >\n sequencing libraries, which were analyzed by Agilent\n Tapestation<sup>®</sup>\n , confirming enrichment of mononucleosomes (~300 bp peak\n represents 150 bp nucleosomes + sequencing adapters).\n </span>\n </p>\n </div>\n </div>\n <aside class=\"image-picker__right\">\n <div class=\"image-picker__gallery\">\n <img\n alt=\"14-0050-dna-fragment-size-distribution-analysis\"\n src=\"/content/images/products/kits/14-0050-dna-fragment-size-distribution-analysis.jpeg\"\n width=\"200\"\n class=\"image-picker__side-image image-picker__side-image_active\"\n role=\"button\"\n />\n </div>\n </aside>\n </section>\n </div>\n </div>\n </div>\n <div id=\"prodAccordion\">\n <div id=\"ProductDescription\" class=\"Block Panel\">\n <h3 class=\"sub-title1\">Technical Information</h3>\n <div\n class=\"ProductDescriptionContainer product-droppdown__section-description\"\n >\n <div class=\"product-tech-info\">\n <div class=\"product-tech-info__line-item\">\n <div class=\"product-tech-info__line-item-left\">\n <b>Kit Contents</b>\n </div>\n <div class=\"product-tech-info__line-item-right\">\n <p style=\"margin: 0\">\n 50 DNA Cleanup Columns + Collection Tubes\n </p>\n <p style=\"margin: 0\">DNA Binding Buffer</p>\n <p style=\"margin: 0\">\n DNA Wash Buffer - Add 20 mL ≥ 95% EtOH prior to use\n </p>\n <p style=\"margin: 0\">DNA Elution Buffer</p>\n </div>\n </div>\n <div class=\"product-tech-info__line-item\">\n <div class=\"product-tech-info__line-item-left\">\n <b>Storage and Stability</b>\n </div>\n <div class=\"product-tech-info__line-item-right\">\n Store at room temperature. Stable for 6 months from date of\n shipment.\n </div>\n </div>\n <div class=\"product-tech-info__line-item\">\n <div class=\"product-tech-info__line-item-left\">\n <b>Instructions for use</b>\n </div>\n <div class=\"product-tech-info__line-item-right\">\n See\n <a\n href=\"/resources/protocols/cutana-dna-purification-kit-manual\"\n >kit manual</a\n >\n for complete instructions.\n </div>\n </div>\n </div>\n </div>\n </div>\n </div>\n <div id=\"prodAccordion\">\n <div id=\"ProductDescription\" class=\"Block Panel\">\n <h3 class=\"sub-title1\">Documents & Resources</h3>\n <div\n class=\"ProductDescriptionContainer product-droppdown__section-description\"\n >\n <div class=\"product-documents\">\n <a\n href=\"/content/documents/tds/14-0050.pdf\"\n target=\"_blank\"\n class=\"product-documents__link\"\n >\n <svg\n version=\"1.1\"\n id=\"Layer_1\"\n xmlns=\"http://www.w3.org/2000/svg\"\n xmlns:xlink=\"http://www.w3.org/1999/xlink\"\n x=\"0px\"\n y=\"0px\"\n viewBox=\"0 0 228 240\"\n style=\"enable-background: new 0 0 228 240\"\n xml:space=\"preserve\"\n class=\"product-documents__icon\"\n alt=\"16-0030 Datasheet\"\n >\n <g>\n <path\n class=\"product-documents__svg-pdf\"\n d=\"M191.92,68.77l-47.69-47.69c-1.33-1.33-3.12-2.08-5.01-2.08H45.09C41.17,19,38,22.17,38,26.09v184.36\n c0,3.92,3.17,7.09,7.09,7.09h141.82c3.92,0,7.09-3.17,7.09-7.09V73.8C194,71.92,193.25,70.1,191.92,68.77z M177.65,77.06h-41.7\n v-41.7L177.65,77.06z M178.05,201.59H53.95V34.95h66.92v47.86c0,5.14,4.17,9.31,9.31,9.31h47.86V201.59z\"\n />\n </g>\n <rect\n x=\"20\"\n y=\"112\"\n class=\"product-documents__svg-background\"\n width=\"146\"\n height=\"76\"\n />\n <g>\n <path\n class=\"product-documents__svg-pdf\"\n d=\"M23.83,125.68h22.36c5.29,0,9.41,1.33,12.35,4c2.94,2.67,4.42,6.39,4.42,11.18c0,4.78-1.47,8.51-4.42,11.18\n c-2.94,2.67-7.06,4-12.35,4H34.59v18.29H23.83V125.68z M44.81,147.9c5.38,0,8.07-2.32,8.07-6.97c0-2.39-0.67-4.16-2-5.31\n c-1.33-1.15-3.36-1.73-6.07-1.73H34.59v14.01H44.81z\"\n />\n <path\n class=\"product-documents__svg-pdf\"\n d=\"M69.92,125.68h18.91c5.29,0,9.84,0.97,13.66,2.9c3.82,1.93,6.74,4.72,8.76,8.35\n c2.02,3.63,3.04,7.98,3.04,13.04c0,5.06-1,9.42-3,13.08c-2,3.66-4.91,6.45-8.73,8.38c-3.82,1.93-8.4,2.9-13.73,2.9H69.92V125.68z\n M88.07,165.63c10.35,0,15.52-5.22,15.52-15.66c0-10.4-5.17-15.59-15.52-15.59h-7.38v31.26H88.07z\"\n />\n <path\n class=\"product-documents__svg-pdf\"\n d=\"M122.57,125.68h32.84v8.49h-22.22v11.18h20.84v8.49h-20.84v20.49h-10.63V125.68z\"\n />\n </g>\n </svg>\n <span class=\"product-documents__info\">Technical Datasheet</span>\n </a>\n </div>\n <div class=\"product-documents\">\n <a\n href=\"/content/documents/manuals/dna-purification-kit.pdf\"\n target=\"_blank\"\n class=\"product-documents__link\"\n >\n <svg\n version=\"1.1\"\n id=\"Layer_1\"\n xmlns=\"http://www.w3.org/2000/svg\"\n xmlns:xlink=\"http://www.w3.org/1999/xlink\"\n x=\"0px\"\n y=\"0px\"\n viewBox=\"0 0 228 240\"\n style=\"enable-background: new 0 0 228 240\"\n xml:space=\"preserve\"\n class=\"product-documents__icon\"\n alt=\"16-0030 Datasheet\"\n >\n <g>\n <path\n class=\"product-documents__svg-pdf\"\n d=\"M191.92,68.77l-47.69-47.69c-1.33-1.33-3.12-2.08-5.01-2.08H45.09C41.17,19,38,22.17,38,26.09v184.36\n c0,3.92,3.17,7.09,7.09,7.09h141.82c3.92,0,7.09-3.17,7.09-7.09V73.8C194,71.92,193.25,70.1,191.92,68.77z M177.65,77.06h-41.7\n v-41.7L177.65,77.06z M178.05,201.59H53.95V34.95h66.92v47.86c0,5.14,4.17,9.31,9.31,9.31h47.86V201.59z\"\n />\n </g>\n <rect\n x=\"20\"\n y=\"112\"\n class=\"product-documents__svg-background\"\n width=\"146\"\n height=\"76\"\n />\n <g>\n <path\n class=\"product-documents__svg-pdf\"\n d=\"M23.83,125.68h22.36c5.29,0,9.41,1.33,12.35,4c2.94,2.67,4.42,6.39,4.42,11.18c0,4.78-1.47,8.51-4.42,11.18\n c-2.94,2.67-7.06,4-12.35,4H34.59v18.29H23.83V125.68z M44.81,147.9c5.38,0,8.07-2.32,8.07-6.97c0-2.39-0.67-4.16-2-5.31\n c-1.33-1.15-3.36-1.73-6.07-1.73H34.59v14.01H44.81z\"\n />\n <path\n class=\"product-documents__svg-pdf\"\n d=\"M69.92,125.68h18.91c5.29,0,9.84,0.97,13.66,2.9c3.82,1.93,6.74,4.72,8.76,8.35\n c2.02,3.63,3.04,7.98,3.04,13.04c0,5.06-1,9.42-3,13.08c-2,3.66-4.91,6.45-8.73,8.38c-3.82,1.93-8.4,2.9-13.73,2.9H69.92V125.68z\n M88.07,165.63c10.35,0,15.52-5.22,15.52-15.66c0-10.4-5.17-15.59-15.52-15.59h-7.38v31.26H88.07z\"\n />\n <path\n class=\"product-documents__svg-pdf\"\n d=\"M122.57,125.68h32.84v8.49h-22.22v11.18h20.84v8.49h-20.84v20.49h-10.63V125.68z\"\n />\n </g>\n </svg>\n <span class=\"product-documents__info\"\n >DNA Purification Kit Manual</span\n >\n </a>\n </div>\n </div>\n </div>\n </div>\n </div>\n</div>\n","detail_messages":"","gift_wrapping_available":false,"gtin":null,"id":"773","images":[{"alt":"CUTANA™ DNA Purification Kit","data":"https://cdn11.bigcommerce.com/s-y9o92/images/stencil/{:size}/products/773/999/Epicypher_CUTANA_DNA_Purification_Box__48142.1646406779.png?c=2"}],"main_image":{"alt":"CUTANA™ DNA Purification 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Provides all necessary components for 50 samples.","meta_keywords":"DNA purification, DNA isolation, DNA extraction, CUT&RUN DNA, CUTANA DNA Purification kit, ","min_purchase_quantity":0,"mpn":null,"num_reviews":0,"options":[],"page_title":"CUTANA™ DNA Purification Kit","price":{"tax_label":"Sales Tax","without_tax":{"currency":"USD","formatted":"$205.00","value":205}},"rating":0,"related_products":[{"add_to_cart_url":"https://www.epicypher.com/cart.php?action=add&product_id=748","add_to_wishlist_url":"/wishlist.php?action=add&product_id=748","availability":"","brand":{"name":null},"category":["Epigenetics Kits and Reagents","Epigenetics Kits and Reagents/CUTANA™ ChIC / CUT&RUN Assays"],"custom_fields":[{"id":510,"name":"Pack size","value":"100 ng"}],"date_added":"14th May 2020","demo":false,"has_options":false,"id":748,"image":{"alt":"CUTANA™ E. coli Spike-in 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samples

Description

Cleavage Under Targets & Release Using Nuclease (CUT&RUN) utilizes an immunotethering approach to specifically release genomic fragments of interest into solution, enabling next-generation sequencing and genomic mapping with unprecedented sensitivity. High yield purification and concentration of partitioned chromatin DNA is an essential step - one that requires a workflow specifically compatible with low starting material. Utilizing tapered cleanup columns designed for low elution volumes (as low as 6 µL), this kit produces purified, concentrated DNA ready for library preparation and next-generation sequencing (Figure 1). The user-friendly workflow can be completed in just 30 minutes. The columns recover > 50 bp DNA fragments, making the DNA Purification Kit ideal for a variety of CUT&RUN targets, including histone PTMs and transcription factors.

Validation Data

14-0050-dna-fragment-size-distribution-analysis

(Click to enlarge)

Figure 1: CUT&RUN DNA fragment size distribution analysis
500,000 K562 cells per sample were processed with the CUTANA™ ChIC/CUT&RUN Kit (EpiCypher 14-1048) following treatment with IgG negative control (EpiCypher 13-0042), H3K4me3 (EpiCypher 13-0041), H3K27me3 (EpiCypher 13-0030) and CTCF (EMD Millipore Catalog No. 07-729) antibodies. Isolated DNA was used to prepare paired-end Illumina^® sequencing libraries, which were analyzed by Agilent Tapestation^® , confirming enrichment of mononucleosomes (~300 bp peak represents 150 bp nucleosomes + sequencing adapters).