CUTANA™ DNA Purification Kit

SKU: 14-0050
Pack size: 50 samples


Cleavage Under Targets & Release Using Nuclease (CUT&RUN) utilizes an immunotethering approach to specifically release genomic fragments of interest into solution, enabling next-generation sequencing and genomic mapping with unprecedented sensitivity. High yield purification and concentration of partitioned chromatin DNA is an essential step - one that requires a workflow specifically compatible with low starting material. Utilizing tapered cleanup columns designed for low elution volumes (as low as 6 µL), this kit produces purified, concentrated DNA ready for library preparation and next-generation sequencing (Figure 1). The user-friendly workflow can be completed in just 30 minutes. The columns recover > 50 bp DNA fragments, making the DNA Purification Kit ideal for a variety of CUT&RUN targets, including histone PTMs and transcription factors.

Validation Data

Figure 1: CUT&RUN DNA fragment size distribution analysis
500,000 K562 cells per sample were processed with the CUTANA™ ChIC/CUT&RUN Kit (EpiCypher 14-1048) following treatment with IgG negative control (EpiCypher 13-0042), H3K4me3 (EpiCypher 13-0041), H3K27me3 (EpiCypher 13-0030) and CTCF (EpiCypher 13-2014) antibodies. Isolated DNA was used to prepare paired-end Illumina® sequencing libraries, which were analyzed by Agilent TapeStation® , confirming enrichment of mononucleosomes (~300 bp peak represents ~170 bp excised DNA + sequencing adapters).

Technical Information

Kit Contents

50 DNA Cleanup Columns + Collection Tubes

DNA Binding Buffer - Add 6.9 mL Isopropanol prior to use

DNA Wash Buffer - Add 20 mL ≥ 95% EtOH prior to use

DNA Elution Buffer

Storage and Stability
Store at room temperature. Stable for 6 months from date of shipment.
Instructions for use
See kit manual for complete instructions.

Documents & Resources

Kit Version 1

Lots 20226001-53, 20335002, and 21130003-02

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