SKU: 14-0050
{"AddThisServiceButtonMeta":"","add_this":[{"annotation":"","service":"facebook"},{"annotation":"","service":"email"},{"annotation":"","service":"print"},{"annotation":"","service":"twitter"},{"annotation":"","service":"linkedin"}],"add_to_wishlist_url":"/wishlist.php?action=add&product_id=773","availability":"","bulk_discount_rates":[],"can_purchase":true,"cart_url":"https://www.epicypher.com/cart.php","category":["Epigenetics Reagents and Assays","Epigenetics Reagents and Assays/CUTANA™ ChIC / CUT&RUN Assays"],"custom_fields":[{"id":"589","name":"Pack size","value":"50 samples"}],"customizations":[],"description":"<table width=\"100%\" border=\"0\" cellspacing=\"2\" cellpadding=\"2\"> <tbody><tr valign=\"top\"><td><table width=\"90%\" border=\"0\" cellspacing=\"1\" cellpadding=\"1\"><tbody><tr><td valign=\"top\"><span style=\"font-family: Arial; \" class=\"Apple-style-span\"><span style=\"font-size: 12pt; \" class=\"Apple-style-span\"><span class=\"Apple-style-span\" style=\"font-family: Arial; \"><span class=\"Apple-style-span\" style=\"font-size: 12pt; \"><b>CUTANA™ DNA Purification Kit Description:</b> Cleavage Under Targets & Release Using Nuclease (CUT&RUN) utilizes an immunotethering approach to specifically release genomic fragments of interest into solution, enabling next-generation sequencing and genomic mapping with unprecedented sensitivity. High yield purification and concentration of partitioned chromatin is an essential step; one that requires a workflow specifically compatible with low starting material. Utilizing tapered cleanup columns designed for low elution volumes (as low as 6 µL), this kit produces purified, concentrated DNA ready for library preparation and next-generation sequencing (<strong>Figure 1</strong>). The user-friendly workflow can be completed in just 30 minutes. The columns recover > 50 bp DNA fragments, making the DNA Purification Kit ideal for a variety of CUT&RUN targets, including histone PTMs and transcription factors. <br /><br /></span></span></td></tr><tr>\n \n <td valign=\"top\"><span style=\"font-family: Arial; \" class=\"Apple-style-span\"><span style=\"font-size: 12pt; \" class=\"Apple-style-span\"><b><span class=\"Apple-style-span\" style=\"font-family: Arial; \"><span class=\"Apple-style-span\" style=\"font-size: 12pt; \"><b>CUTANA™ DNA Purification Kit</b></span></span> Contents:</b> 50 DNA Cleanup Columns + Collection Tubes</br>\n\nDNA Binding Buffer</br>\n\nDNA Wash Buffer - <i>Add 20 mL ≥ 95% EtOH prior to use</i></br>\n\nDNA Elution Buffer<br /><br /></span></span></td></tr><tr>\n \n <td valign=\"top\"><span style=\"font-family: Arial; \" class=\"Apple-style-span\"><span style=\"font-size: 12pt; \" class=\"Apple-style-span\"><b>CUTANA™ DNA Purification Kit Storage and Stability:</b> Store at room temperature. Stable for 6 months from date of shipment. <br /><br /></span></span></td></tr><tr>\n \n <td valign=\"top\"><span style=\"font-family: Arial; \" class=\"Apple-style-span\"><span style=\"font-size: 12pt; \" class=\"Apple-style-span\"><b>CUTANA™ DNA Purification Kit Instructions for use:</b> See kit manual for complete instructions.<br /><br /></span></span></td></tr><tr>\n\t \n\t<td valign=\"top\"><span style=\"font-family: Arial; \" class=\"Apple-style-span\"><span style=\"font-size: 12pt; \" class=\"Apple-style-span\">View technical datasheet for this product. <a href=\"/content/documents/tds/14-0050.pdf\" target=\"_new\"><img src=\"/content/documents/tds/icon.png\" alt=\"14-0050 Datasheet\" width=\"30\" height=\"40\" /></a></span></span></td></tr><tr>\t\n\t\n\t<td valign=\"top\"><span style=\"font-family: Arial; \" class=\"Apple-style-span\"><span style=\"font-size: 12pt; \" class=\"Apple-style-span\">View Kit Manual. <a href=\"/content/documents/manuals/dna-purification-kit.pdf\" target=\"_new\"><img src=\"/content/documents/tds/icon.png\" alt=\"CUTANA ChIC/CUT&RUN Kit Manual\" width=\"30\" height=\"40\" /></a></span></span></td></tr><tr></tbody></table></td>\n\t\n\t\n\n\n<td><table width=\"100%\" border=\"0\" cellspacing=\"3\" cellpadding=\"3\"><tbody><tr><td align=\"center\"><a href=\"/content/images/products/kits/14-0050-DFSDA.jpg\" target=\"_new\"><img src=\"/content/images/products/kits/14-0050-DFSDA.jpg\" alt=\"14-0050 DNA Fragment Size Distribution Analysis\" width=\"125\" height=\"325\" /></a> </td></tr><tr><td align=\"left\" valign=\"top\"> <span style=\"font-family: Arial; \" class=\"Apple-style-span\"><span style=\"font-size: 10pt; \" class=\"Apple-style-span\"><strong>CUT&RUN DNA Fragment Size Distribution Analysis:</strong>CUT&RUN was performed using the CUTANA ChIC/CUT&RUN Kit starting with 500,000 K562 cells. CUT&RUN DNA isolated from IgG Negative Control (13-0042k) and H3K4me3 Positive Control (13-0041k) antibodies was used to prepare paired-end Illumina sequencing libraries. Library DNA was analyzed by Agilent Tapestation®. This analysis confirmed that mononucleosomes were predominantly enriched in CUT&RUN (~300 bp peak represents 150 bp nucleosomes + sequencing adapters). <br /> <strong>(Click image to enlarge) </strong></span></span></p></td></tr></tbody></table>\n\n </td></tr>\n\n\n\n\n</tbody></table></td></tr>\n\n\n\n</tbody></table> </td></tr></tbody></table>","detail_messages":"","gift_wrapping_available":false,"id":"773","images":[{"alt":"CUTANA™ DNA Purification Kit","data":"https://cdn11.bigcommerce.com/s-y9o92/images/stencil/{:size}/products/773/760/2020.03.31_CUTRUN_Kit_Box_AJ__12101.1597331219.1280.1280__13283.1603294945.jpg?c=2"}],"main_image":{"alt":"CUTANA™ DNA Purification Kit","data":"https://cdn11.bigcommerce.com/s-y9o92/images/stencil/{:size}/products/773/760/2020.03.31_CUTRUN_Kit_Box_AJ__12101.1597331219.1280.1280__13283.1603294945.jpg?c=2"},"max_purchase_quantity":0,"meta_description":"CUTANA™ DNA Purification Kit is validated for CUT&RUN DNA isolation and purification. Provides all necessary components for 50 samples.","meta_keywords":"DNA purification, DNA isolation, DNA extraction, CUT&RUN DNA, CUTANA DNA Purification kit, ","min_purchase_quantity":0,"num_reviews":0,"options":[],"page_title":"CUTANA™ DNA Purification Kit","price":{"tax_label":"Sales Tax","without_tax":{"currency":"USD","formatted":"$205.00","value":205}},"rating":0,"related_products":[{"add_to_cart_url":"https://www.epicypher.com/cart.php?action=add&product_id=748","add_to_wishlist_url":"/wishlist.php?action=add&product_id=748","availability":"","brand":{"name":null},"category":["Epigenetics Reagents and Assays","Epigenetics Reagents and Assays/CUTANA™ ChIC / CUT&RUN Assays"],"custom_fields":[{"id":510,"name":"Pack size","value":"100 ng"}],"date_added":"14th May 2020","demo":false,"has_options":false,"id":748,"image":{"alt":"CUTANA™ E. coli Spike-in DNA","data":"https://cdn11.bigcommerce.com/s-y9o92/images/stencil/{:size}/products/748/730/DNA_Vector_Draw_Final-w-border-01__76823.1592491202.png?c=2"},"images":[{"alt":"CUTANA™ E. coli Spike-in DNA","data":"https://cdn11.bigcommerce.com/s-y9o92/images/stencil/{:size}/products/748/730/DNA_Vector_Draw_Final-w-border-01__76823.1592491202.png?c=2"}],"low_stock_level":null,"name":"CUTANA™ E. coli Spike-in DNA","num_reviews":null,"pre_order":false,"price":{"tax_label":"Sales Tax","without_tax":{"currency":"USD","formatted":"$105.00","value":105}},"qty_in_cart":0,"rating":null,"show_cart_action":true,"sku":"18-1401","stock_level":null,"summary":"\n\n\nUntitled Document\n\n\n\n\n CUTANA E. coli Spike-in DNA Description: Fragmented DNA derived from Escherichia coli (E. coli) can be used as a spike-in control for experimental normalization in...","url":"https://www.epicypher.com/products/epigenetics-reagents-and-assays/cutana-e-coli-spike-in-dna","weight":{"formatted":"0.01 LBS","value":0.01}},{"add_to_cart_url":"https://www.epicypher.com/cart.php?action=add&product_id=772","add_to_wishlist_url":"/wishlist.php?action=add&product_id=772","availability":"","brand":{"name":null},"category":["Epigenetics Reagents and Assays","Epigenetics Reagents and Assays/CUTANA™ ChIC / CUT&RUN Assays"],"custom_fields":[{"id":588,"name":"Pack size","value":"48 Reactions"}],"date_added":"13th Aug 2020","demo":false,"has_options":false,"id":772,"image":{"alt":"CUTANA™ ChIC/CUT&RUN Kit","data":"https://cdn11.bigcommerce.com/s-y9o92/images/stencil/{:size}/products/772/759/2020.03.31_CUTRUN_Kit_Box_AJ__12101.1597331219.png?c=2"},"images":[{"alt":"CUTANA™ ChIC/CUT&RUN Kit","data":"https://cdn11.bigcommerce.com/s-y9o92/images/stencil/{:size}/products/772/759/2020.03.31_CUTRUN_Kit_Box_AJ__12101.1597331219.png?c=2"}],"low_stock_level":null,"name":"CUTANA™ ChIC/CUT&RUN Kit","num_reviews":null,"pre_order":false,"price":{"tax_label":"Sales Tax","without_tax":{"currency":"USD","formatted":"$850.00","value":850}},"qty_in_cart":0,"rating":null,"show_cart_action":true,"sku":"14-1048","stock_level":null,"summary":" CUTANA™ ChIC/CUT&RUN Kit Description: The CUTANA ChIC/CUT&RUN Kit contains sufficient materials for 48 CUT&RUN samples and is designed for multi-channel sample pipetting in order to realize the...","url":"https://www.epicypher.com/products/epigenetics-reagents-and-assays/cutana-chic-cut-run-kit","weight":{"formatted":"0.01 LBS","value":0.01}}],"shipping":{"calculated":true},"shipping_messages":[],"show_quantity_input":1,"sku":"14-0050","tags":[],"title":"CUTANA™ DNA Purification Kit","upc":null,"url":"https://www.epicypher.com/products/epigenetics-reagents-and-assays/cutana-dna-purification-kit","warranty":"","weight":"0.10 LBS"}
Pack size: 50 samples
