CUTANA™ DNA Purification Kit

SKU: 14-0050
Pack size: 50 samples
CUTANA™ DNA Purification Kit Description: Cleavage Under Targets & Release Using Nuclease (CUT&RUN) utilizes an immunotethering approach to specifically release genomic fragments of interest into solution, enabling next-generation sequencing and genomic mapping with unprecedented sensitivity. High yield purification and concentration of partitioned chromatin is an essential step; one that requires a workflow specifically compatible with low starting material. Utilizing tapered cleanup columns designed for low elution volumes (as low as 6 µL), this kit produces purified, concentrated DNA ready for library preparation and next-generation sequencing (Figure 1). The user-friendly workflow can be completed in just 30 minutes. The columns recover > 50 bp DNA fragments, making the DNA Purification Kit ideal for a variety of CUT&RUN targets, including histone PTMs and transcription factors.

CUTANA™ DNA Purification Kit Contents: 50 DNA Cleanup Columns + Collection Tubes
DNA Binding Buffer
DNA Wash Buffer - Add 20 mL ≥ 95% EtOH prior to use
DNA Elution Buffer

CUTANA™ DNA Purification Kit Storage and Stability: Store at room temperature. Stable for 6 months from date of shipment.

CUTANA™ DNA Purification Kit Instructions for use: See kit manual for complete instructions.

View technical datasheet for this product. 14-0050 Datasheet
View Kit Manual. CUTANA ChIC/CUT&RUN Kit Manual
14-0050 DNA Fragment Size Distribution Analysis
CUT&RUN DNA Fragment Size Distribution Analysis:CUT&RUN was performed using the CUTANA ChIC/CUT&RUN Kit starting with 500,000 K562 cells. CUT&RUN DNA isolated from IgG Negative Control (13-0042k) and H3K4me3 Positive Control (13-0041k) antibodies was used to prepare paired-end Illumina sequencing libraries. Library DNA was analyzed by Agilent Tapestation®. This analysis confirmed that mononucleosomes were predominantly enriched in CUT&RUN (~300 bp peak represents 150 bp nucleosomes + sequencing adapters).
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