SKU: 14-0052
Size/Quantity: 1 Kit

CUTANA™ Quick Cleanup DNA Purification Kit

$295.00

46 in stock

The CUTANA™ Quick Cleanup DNA Purification Kit can be used to purify CUT&RUN or CUT&Tag DNA and remove unwanted adapter-/primer-dimers from these reactions. Unlike many kits that purify DNA using spin columns, this kit uses SPRI paramagnetic beads for streamlined, higher throughput workflows easily integrated into CUTANA™ genomic mapping assays. Depending on the application, various SPRI bead:DNA ratios are added to capture target DNA.

In CUT&RUN, pAG-MNase cleaves DNA proximal to antibody-labeled chromatin, releasing it into the supernatant. It is imperative to recover the highest DNA yield possible from the supernatant; therefore, a higher bead:DNA ratio is added to span a wide range of DNA fragment lengths. This kit includes sufficient volume to perform 48 total CUT&RUN reactions at an empirically optimized higher bead ratio.

In CUT&Tag, pAG-Tn5 cleaves antibody-bound chromatin and simultaneously ligates adapter DNA, resulting in longer DNA fragments. Further, in the EpiCypher CUTANA™ Direct-to-PCR CUT&Tag workflow, indexing PCR is used to amplify next-generation sequencing (NGS) libraries directly from the reaction mixture prior to DNA purification. In this case, a lower bead ratio is ideal for preferable enrichment of longer DNA fragments and selective cleanup of sequencing libraries. This kit includes sufficient volume to perform 96 total CUT&Tag reactions at an empirically optimized lower bead ratio.

This kit can also be used to cleanup NGS libraries by removing contaminating small fragments such as adapter dimers in CUT&RUN (~150 bp) and primer dimers in CUT&Tag (~25-75 bp).

Beckman Coulter, the stylized logo, and SPRIselect are trademarks or registered trademarks of Beckman Coulter, Inc. in the United States and other countries.
Storage
Store at room temperature. Stable for 1 year upon date of receipt.
 
Instructions for Use
This kit contains sufficient reagents to purify 48 CUT&RUN reactions -OR- 96 CUT&Tag reactions. A lower bead:DNA ratio is required to purify CUT&Tag DNA versus CUT&RUN DNA, therefore allowing for more CUT&Tag purifications. This kit can also be used to cleanup NGS libraries by removing adapter dimers in CUT&RUN and primer dimers in CUT&Tag. See Kit Manual corresponding to Kit Version 1 for detailed protocols.
ItemCat. No.
CUTANA™ 8-strip 0.2 mL Tubes10-0009d
SPRIselect reagent manufactured by Beckman Coulter, Inc.21-1405d
0.1X TE Buffer21-1025d
Item Cat. No.
CUTANA™ CUT&RUN Library Prep Kit 14-1001/14-1002
CUTANA™ ChIC/CUT&RUN Kit 14-1048/14-1048-24
CUTANA™ CUT&Tag Kit 14-1102/14-1103
CUTANA™ meCUT&RUN Kit for DNA Methylation Sequencing 14-1060-24
Magnetic Separation Rack, 0.2 mL Tubes 10-0008
Magnetic Separation Rack, 1.5 mL Tubes 10-0012
CUTANA™ Nuclei Extraction Buffer 21-1026
CUTANA™ Protease Inhibitor Tablets 21-1027
Kit Version 1

Technical Datasheet

Quick Cleanup DNA Purification Kit Manual

Quick Cleanup DNA Purification Kit Quick Card

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