

CTCF CUTANA™ CUT&RUN Antibody
{"AddThisServiceButtonMeta":"","add_this":[{"annotation":"","service":"facebook"},{"annotation":"","service":"email"},{"annotation":"","service":"print"},{"annotation":"","service":"twitter"},{"annotation":"","service":"linkedin"}],"add_to_wishlist_url":"/wishlist.php?action=add&product_id=874","availability":"","bulk_discount_rates":[],"can_purchase":false,"cart_url":"https://www.epicypher.com/cart.php","category":["Antibodies/CUTANA™ CUT&RUN Antibodies","Antibodies/CUTANA™ CUT&RUN Antibodies/CUT&RUN Antibodies - Chromatin-Associated Proteins","Epigenetics Kits and Reagents/CUTANA™ ChIC / CUT&RUN Assays"],"custom_fields":[{"id":"908","name":"Pack Size","value":"100 µL"}],"customizations":[],"description":"<div class=\"product-general-info\">\n <ul class=\"product-general-info__list-left\">\n <li class=\"product-general-info__list-item\">\n <strong>Type: </strong>Monoclonal [BLR041F]\n </li>\n <li class=\"product-general-info__list-item\">\n <strong>Host: </strong>Rabbit\n </li>\n <li class=\"product-general-info__list-item\">\n <strong>Target Size: </strong>83 kDa\n </li>\n </ul>\n <ul class=\"product-general-info__list-right\">\n <li class=\"product-general-info__list-item\">\n <strong>Format: </strong>Purified IgG\n </li>\n <li class=\"product-general-info__list-item\">\n <strong>Reactivity: </strong>Human, Mouse\n </li>\n <li class=\"product-general-info__list-item\">\n <strong>Applications: </strong>CUT&RUN, WB, IP, IHC, ICC\n </li>\n </ul>\n</div>\n<div class=\"service_accordion product-droppdown\">\n <div class=\"container\">\n <div id=\"prodAccordion\">\n <div id=\"ProductDescription\" class=\"Block Panel current\">\n <h3 class=\"sub-title1\">Description</h3>\n <div\n class=\"\n ProductDescriptionContainer\n product-droppdown__section-description-specific\n \"\n >\n <p>\n This CTCF antibody meets EpiCypher’s “CUTANA Compatible” criteria for\n performance in Cleavage Under Targets and Release Using Nuclease\n (CUT&RUN) and/or Cleavage Under Targets and Tagmentation\n (CUT&Tag) approaches to genomic mapping. Every lot of a CUTANA\n Compatible antibody is tested in the indicated CUTANA approach using\n <a href=\"/resources/protocols\">EpiCypher optimized protocols </a>\n and determined to yield peaks that show a genomic distribution\n pattern consistent with reported function(s) of the target protein.\n CTCF antibody produces CUT&RUN peaks at promoters\n <strong>(Figure 1A)</strong> and to a greater extent within\n intergenic and intronic regions <strong>(Figure 1B)</strong>.\n CUT&RUN peaks overlap with known CTCF transcription factor binding\n motifs <strong>(Figure 2)</strong>.\n </p>\n </div>\n </div>\n </div>\n <div id=\"prodAccordion\">\n <div id=\"ProductDescription\" class=\"Block Panel current\">\n <h3 class=\"sub-title1\">Validation Data</h3>\n <div\n class=\"\n ProductDescriptionContainer\n product-droppdown__section-description-specific\n \"\n >\n <section class=\"image-picker\">\n <div class=\"image-picker__left\">\n <div\n class=\"\n image-picker__main-content_active image-picker__main-content\n \"\n >\n <div class=\"image-picker__header-content\">\n <button class=\"image-picker__left-arrow\">\n <svg\n class=\"image-picker__svg-left\"\n width=\"24\"\n height=\"24\"\n viewBox=\"0 0 24 24\"\n >\n <path\n d=\"M16.67 0l2.83 2.829-9.339 9.175 9.339 9.167-2.83 2.829-12.17-11.996z\"\n />\n </svg>\n </button>\n <a\n href=\"/content/images/products/antibodies/13-2014-CTCF-Heatmap.jpg\"\n target=\"_new\"\n class=\"image-picker__main-image-link\"\n ><img\n alt=\"13-2014-CTCF-Heatmap\"\n src=\"/content/images/products/antibodies/13-2014-CTCF-Heatmap.jpg\"\n class=\"image-picker__main-image\"\n />\n <span class=\"image-picker__main-image-caption\"\n >(Click to enlarge)</span\n ></a\n >\n <button class=\"image-picker__right-arrow\">\n <svg\n class=\"image-picker__svg-right\"\n width=\"24\"\n height=\"24\"\n viewBox=\"0 0 24 24\"\n >\n <path\n d=\"M7.33 24l-2.83-2.829 9.339-9.175-9.339-9.167 2.83-2.829 12.17 11.996z\"\n />\n </svg>\n </button>\n </div>\n <p>\n <span class=\"image-picker__span-content\"\n ><strong\n >Figure 1: CTCF signal enrichment in CUT&RUN </strong\n ><br />\n CUT&RUN was performed using 500,000 K562 cells with CTCF\n antibody (0.125 µg) and IgG control (0.5 µg, EpiCypher\n 13-0042). <strong>(A)</strong> Sequencing reads were aligned\n to annotated TSSs (+/- 2 kbp) of 18,793 genes. High, medium,\n and low signal is ranked by intensity (top to bottom;\n aligned relative to CTCF) and reflected by red, yellow, and\n blue colors, respectively. <strong>(B)</strong> CTCF peaks\n were called using SEACR. The number of CTCF peaks which fall\n into distinct classes of functionally annotated genomic\n regions is plotted.\n </span>\n </p>\n </div>\n <div class=\"image-picker__main-content\">\n <div class=\"image-picker__header-content\">\n <button class=\"image-picker__left-arrow\">\n <svg\n class=\"image-picker__svg-left\"\n width=\"24\"\n height=\"24\"\n viewBox=\"0 0 24 24\"\n >\n <path\n d=\"M16.67 0l2.83 2.829-9.339 9.175 9.339 9.167-2.83 2.829-12.17-11.996z\"\n />\n </svg>\n </button>\n <a\n href=\"/content/images/products/antibodies/13-2014-CTCF-Motif.jpg\"\n target=\"_new\"\n class=\"image-picker__main-image-link\"\n ><img\n alt=\"13-2014-CTCF-Motif\"\n src=\"/content/images/products/antibodies/13-2014-CTCF-Motif.jpg\"\n class=\"image-picker__main-image\"\n />\n <span class=\"image-picker__main-image-caption\"\n >(Click to enlarge)</span\n ></a\n >\n <button class=\"image-picker__right-arrow\">\n <svg\n class=\"image-picker__svg-right\"\n width=\"24\"\n height=\"24\"\n viewBox=\"0 0 24 24\"\n >\n <path\n d=\"M7.33 24l-2.83-2.829 9.339-9.175-9.339-9.167 2.83-2.829 12.17 11.996z\"\n />\n </svg>\n </button>\n </div>\n <p>\n <span class=\"image-picker__span-content\"\n ><strong\n >Figure 2: CTCF transcription factor binding motif\n analysis in CUT&RUN</strong\n ><br />\n <strong>(A)</strong> MEME analysis determined that the CTCF\n consensus motif, represented as a sequence logo position\n weight matrix, was enriched under CUT&RUN peaks produced by CTCF\n antibody. <strong>(B)</strong> The number of CTCF peaks containing\n consensus motifs from panel A is represented by a Venn\n Diagram. <strong>(C)</strong> Three representative loci\n showing CTCF peaks (IGV).\n </span>\n </p>\n </div>\n <div class=\"image-picker__main-content\">\n <div class=\"image-picker__header-content\">\n <button class=\"image-picker__left-arrow\">\n <svg\n class=\"image-picker__svg-left\"\n width=\"24\"\n height=\"24\"\n viewBox=\"0 0 24 24\"\n >\n <path\n d=\"M16.67 0l2.83 2.829-9.339 9.175 9.339 9.167-2.83 2.829-12.17-11.996z\"\n />\n </svg>\n </button>\n <a\n href=\"/content/images/products/antibodies/13-2014-western-blot.jpeg\"\n target=\"_new\"\n class=\"image-picker__main-image-link\"\n ><img\n alt=\"13-2014-western-blot\"\n src=\"/content/images/products/antibodies/13-2014-western-blot.jpeg\"\n class=\"image-picker__main-image\"\n />\n <span class=\"image-picker__main-image-caption\"\n >(Click to enlarge)</span\n ></a\n >\n <button class=\"image-picker__right-arrow\">\n <svg\n class=\"image-picker__svg-right\"\n width=\"24\"\n height=\"24\"\n viewBox=\"0 0 24 24\"\n >\n <path\n d=\"M7.33 24l-2.83-2.829 9.339-9.175-9.339-9.167 2.83-2.829 12.17 11.996z\"\n />\n </svg>\n </button>\n </div>\n <p>\n <span class=\"image-picker__span-content\"\n ><strong\n >Figure 3: Western blot detection of human and mouse\n CTCF</strong\n ><br />\n Whole cell lysates were isolated from HEK293T (293T), MCF-7,\n Hep-G2, A-549, SW620, SK-MEL-28 (SK), Jurkat, TCMK-1, and\n NIH 3T3 cells using NETN lysis buffer. Fifty micrograms (50\n µg) of each lysate was loaded onto an SDS-PAGE gel and\n analyzed under standard western blot conditions using CTCF\n antibody (1;1,000).\n </span>\n </p>\n </div>\n <div class=\"image-picker__main-content\">\n <div class=\"image-picker__header-content\">\n <button class=\"image-picker__left-arrow\">\n <svg\n class=\"image-picker__svg-left\"\n width=\"24\"\n height=\"24\"\n viewBox=\"0 0 24 24\"\n >\n <path\n d=\"M16.67 0l2.83 2.829-9.339 9.175 9.339 9.167-2.83 2.829-12.17-11.996z\"\n />\n </svg>\n </button>\n <a\n href=\"/content/images/products/antibodies/13-2014-IP-CTCF.jpeg\"\n target=\"_new\"\n class=\"image-picker__main-image-link\"\n ><img\n alt=\"13-2014-IP-CTCF\"\n src=\"/content/images/products/antibodies/13-2014-IP-CTCF.jpeg\"\n class=\"image-picker__main-image\"\n />\n <span class=\"image-picker__main-image-caption\"\n >(Click to enlarge)</span\n ></a\n >\n <button class=\"image-picker__right-arrow\">\n <svg\n class=\"image-picker__svg-right\"\n width=\"24\"\n height=\"24\"\n viewBox=\"0 0 24 24\"\n >\n <path\n d=\"M7.33 24l-2.83-2.829 9.339-9.175-9.339-9.167 2.83-2.829 12.17 11.996z\"\n />\n </svg>\n </button>\n </div>\n <p>\n <span class=\"image-picker__span-content\"\n ><strong>Figure 4: Immunoprecipitation of human CTCF</strong\n ><br />\n EpiCypher CTCF antibody (20 µL) was used to\n immunoprecipitate whole cell lysates isolated from HEK293T\n cells using NETN lysis buffer (1 mg per IP). A negative\n control IgG antibody and a different CTCF positive control\n antibody (Bethyl Laboratories) were also used to demonstrate\n specificity of the IP. Immunoprecipitates were loaded onto\n an SDS-PAGE gel (20% of IP loaded) and probed via western\n blot with EpiCypher CTCF antibody (1:1,000).\n </span>\n </p>\n </div>\n <div class=\"image-picker__main-content\">\n <div class=\"image-picker__header-content\">\n <button class=\"image-picker__left-arrow\">\n <svg\n class=\"image-picker__svg-left\"\n width=\"24\"\n height=\"24\"\n viewBox=\"0 0 24 24\"\n >\n <path\n d=\"M16.67 0l2.83 2.829-9.339 9.175 9.339 9.167-2.83 2.829-12.17-11.996z\"\n />\n </svg>\n </button>\n <a\n href=\"/content/images/products/antibodies/13-2014-IHC-CTCF.jpeg\"\n target=\"_new\"\n class=\"image-picker__main-image-link\"\n ><img\n alt=\"13-2014-IHC-CTCF\"\n src=\"/content/images/products/antibodies/13-2014-IHC-CTCF.jpeg\"\n class=\"image-picker__main-image\"\n />\n <span class=\"image-picker__main-image-caption\"\n >(Click to enlarge)</span\n ></a\n >\n <button class=\"image-picker__right-arrow\">\n <svg\n class=\"image-picker__svg-right\"\n width=\"24\"\n height=\"24\"\n viewBox=\"0 0 24 24\"\n >\n <path\n d=\"M7.33 24l-2.83-2.829 9.339-9.175-9.339-9.167 2.83-2.829 12.17 11.996z\"\n />\n </svg>\n </button>\n </div>\n <p>\n <span class=\"image-picker__span-content\"\n ><strong\n >Figure 5: Immunohistochemistry detection of human\n CTCF</strong\n ><br />\n FFPE section of human lung carcinoma examined using CTCF\n antibody (1:100 dilution).\n </span>\n </p>\n </div>\n <div class=\"image-picker__main-content\">\n <div class=\"image-picker__header-content\">\n <button class=\"image-picker__left-arrow\">\n <svg\n class=\"image-picker__svg-left\"\n width=\"24\"\n height=\"24\"\n viewBox=\"0 0 24 24\"\n >\n <path\n d=\"M16.67 0l2.83 2.829-9.339 9.175 9.339 9.167-2.83 2.829-12.17-11.996z\"\n />\n </svg>\n </button>\n <a\n href=\"/content/images/products/antibodies/13-2014-ICC-CTCF.jpeg\"\n target=\"_new\"\n class=\"image-picker__main-image-link\"\n ><img\n alt=\"13-2014-ICC-CTCF\"\n src=\"/content/images/products/antibodies/13-2014-ICC-CTCF.jpeg\"\n class=\"image-picker__main-image\"\n />\n <span class=\"image-picker__main-image-caption\"\n >(Click to enlarge)</span\n ></a\n >\n <button class=\"image-picker__right-arrow\">\n <svg\n class=\"image-picker__svg-right\"\n width=\"24\"\n height=\"24\"\n viewBox=\"0 0 24 24\"\n >\n <path\n d=\"M7.33 24l-2.83-2.829 9.339-9.175-9.339-9.167 2.83-2.829 12.17 11.996z\"\n />\n </svg>\n </button>\n </div>\n <p>\n <span class=\"image-picker__span-content\"\n ><strong\n >Figure 6: Immunocytochemistry detection of human\n CTCF</strong\n ><br />\n FFPE section of human MCF-7 cells examined using CTCF\n antibody (1:100 dilution).\n </span>\n </p>\n </div>\n </div>\n <aside class=\"image-picker__right\">\n <div class=\"image-picker__gallery\">\n <img\n alt=\"13-2014-CTCF-Heatmap\"\n src=\"/content/images/products/antibodies/13-2014-CTCF-Heatmap.jpeg\"\n width=\"200\"\n class=\"\n image-picker__side-image image-picker__side-image_active\n \"\n role=\"button\"\n />\n <img\n alt=\"13-2014-CTCF-Motif\"\n src=\"/content/images/products/antibodies/13-2014-CTCF-Motif.jpg\"\n class=\"image-picker__side-image\"\n role=\"button\"\n />\n <img\n alt=\"13-2014-western-blot\"\n src=\"/content/images/products/antibodies/13-2014-western-blot.jpeg\"\n class=\"image-picker__side-image\"\n role=\"button\"\n />\n <img\n alt=\"13-2014-IP-CTCF\"\n src=\"/content/images/products/antibodies/13-2014-IP-CTCF.jpeg\"\n class=\"image-picker__side-image\"\n role=\"button\"\n />\n <img\n alt=\"13-2014-IHC-CTCF\"\n src=\"/content/images/products/antibodies/13-2014-IHC-CTCF.jpeg\"\n class=\"image-picker__side-image\"\n role=\"button\"\n />\n <img\n alt=\"13-2014-ICC-CTCF\"\n src=\"/content/images/products/antibodies/13-2014-ICC-CTCF.jpeg\"\n class=\"image-picker__side-image\"\n role=\"button\"\n />\n </div>\n </aside>\n </section>\n </div>\n </div>\n </div>\n <div id=\"prodAccordion\">\n <div id=\"ProductDescription\" class=\"Block Panel\">\n <h3 class=\"sub-title1\">Technical Information</h3>\n <div\n class=\"\n ProductDescriptionContainer\n product-droppdown__section-description\n \"\n >\n <div class=\"product-tech-info\">\n <div class=\"product-tech-info__line-item\">\n <div class=\"product-tech-info__line-item-left\">\n <b>Immunogen</b>\n </div>\n\n <div class=\"product-tech-info__line-item-right\">\n A synthetic peptide corresponding to human CTCF (CCCTC-binding\n factor) amino acids 650 to 700.\n </div>\n </div>\n <div class=\"product-tech-info__line-item\">\n <div class=\"product-tech-info__line-item-left\">\n <b>Formulation</b>\n </div>\n <div class=\"product-tech-info__line-item-right\">\n Purified recombinant monoclonal CTCF antibody (100 µg/mL) in borate\n buffered saline pH 8.2, 0.1% BSA, 0.09% sodium azide.\n </div>\n </div>\n <div class=\"product-tech-info__line-item\">\n <div class=\"product-tech-info__line-item-left\">\n <b>Storage and Stability</b>\n </div>\n <div class=\"product-tech-info__line-item-right\">\n Stable for 1 year at 4°C from date of receipt.\n </div>\n </div>\n </div>\n </div>\n </div>\n </div>\n <div id=\"prodAccordion\">\n <div id=\"ProductDescription\" class=\"Block Panel\">\n <h3 class=\"sub-title1\">Application Notes</h3>\n <div\n class=\"\n ProductDescriptionContainer\n product-droppdown__section-description\n \"\n >\n <p><strong>Recommended Dilutions:</strong></p>\n <p><strong>CUT&RUN:</strong> 0.1 - 0.3 µg</p>\n <p><strong>Western Blot (WB):</strong> 1:1,000</p>\n <p><strong>Immunoprecipitation (IP):</strong> 20 µL/mg lysate</p>\n <p><strong>Immunohistochemistry (IHC):</strong> 1:100 - 1:500*</p>\n <p><strong>Immunocytochemistry (ICC):</strong> 1:100 - 1:500*</p>\n <p>\n <em\n >*Epitope retrieval with citrate buffer pH 6.0 recommended for\n FFPE tissues.</em\n >\n </p>\n </div>\n </div>\n </div>\n <!-- <div id=\"prodAccordion\">\n <div id=\"ProductDescription\" class=\"Block Panel\">\n <h3 class=\"sub-title1\">References</h3>\n <div\n class=\"\n ProductDescriptionContainer\n product-droppdown__section-description\n \"\n >\n <strong>Background References:</strong>\n <br />\n 1. Wagner <em>et al.</em> <em>BMC Cancer </em> (2013). PMID:\n <a\n href=\"https://pubmed.ncbi.nlm.nih.gov/24304549/\"\n title=\"NCOA3 is a selective co-activator of estrogen receptor α-mediated transactivation of PLAC1 in MCF-7 breast cancer cells\"\n target=\"new\"\n >\n 24304549</a\n ><br />\n </div>\n </div>\n </div> -->\n <div id=\"prodAccordion\">\n <div id=\"ProductDescription\" class=\"Block Panel\">\n <h3 class=\"sub-title1\">Documents & Resources</h3>\n <div\n class=\"\n ProductDescriptionContainer\n product-droppdown__section-description\n \"\n >\n <div class=\"product-documents\">\n <a\n href=\"/content/documents/tds/13-2014.pdf\"\n target=\"_new\"\n class=\"product-documents__link\"\n >\n <svg\n version=\"1.1\"\n id=\"Layer_1\"\n xmlns=\"http://www.w3.org/2000/svg\"\n xmlns:xlink=\"http://www.w3.org/1999/xlink\"\n x=\"0px\"\n y=\"0px\"\n viewBox=\"0 0 228 240\"\n style=\"enable-background: new 0 0 228 240\"\n xml:space=\"preserve\"\n class=\"product-documents__icon\"\n >\n <g>\n <path\n class=\"product-documents__svg-pdf\"\n d=\"M191.92,68.77l-47.69-47.69c-1.33-1.33-3.12-2.08-5.01-2.08H45.09C41.17,19,38,22.17,38,26.09v184.36\n c0,3.92,3.17,7.09,7.09,7.09h141.82c3.92,0,7.09-3.17,7.09-7.09V73.8C194,71.92,193.25,70.1,191.92,68.77z M177.65,77.06h-41.7\n v-41.7L177.65,77.06z M178.05,201.59H53.95V34.95h66.92v47.86c0,5.14,4.17,9.31,9.31,9.31h47.86V201.59z\"\n />\n </g>\n <rect\n x=\"20\"\n y=\"112\"\n class=\"product-documents__svg-background\"\n width=\"146\"\n height=\"76\"\n />\n <g>\n <path\n class=\"product-documents__svg-pdf\"\n d=\"M23.83,125.68h22.36c5.29,0,9.41,1.33,12.35,4c2.94,2.67,4.42,6.39,4.42,11.18c0,4.78-1.47,8.51-4.42,11.18\n c-2.94,2.67-7.06,4-12.35,4H34.59v18.29H23.83V125.68z M44.81,147.9c5.38,0,8.07-2.32,8.07-6.97c0-2.39-0.67-4.16-2-5.31\n c-1.33-1.15-3.36-1.73-6.07-1.73H34.59v14.01H44.81z\"\n />\n <path\n class=\"product-documents__svg-pdf\"\n d=\"M69.92,125.68h18.91c5.29,0,9.84,0.97,13.66,2.9c3.82,1.93,6.74,4.72,8.76,8.35\n c2.02,3.63,3.04,7.98,3.04,13.04c0,5.06-1,9.42-3,13.08c-2,3.66-4.91,6.45-8.73,8.38c-3.82,1.93-8.4,2.9-13.73,2.9H69.92V125.68z\n M88.07,165.63c10.35,0,15.52-5.22,15.52-15.66c0-10.4-5.17-15.59-15.52-15.59h-7.38v31.26H88.07z\"\n />\n <path\n class=\"product-documents__svg-pdf\"\n d=\"M122.57,125.68h32.84v8.49h-22.22v11.18h20.84v8.49h-20.84v20.49h-10.63V125.68z\"\n />\n </g>\n </svg>\n <span class=\"product-documents__info\">Technical Datasheet</span>\n </a>\n </div>\n </div>\n </div>\n </div>\n <div id=\"prodAccordion\">\n <div id=\"ProductDescription\" class=\"Block Panel\">\n <h3 class=\"sub-title1\">Additional Info</h3>\n <div\n class=\"\n ProductDescriptionContainer\n product-droppdown__section-description\n \"\n >\n <p>\n This CTCF antibody is provided for commercial sale under license from\n Bethyl Laboratories, Inc.\n </p>\n </div>\n </div>\n </div>\n </div>\n</div>\n","detail_messages":"","gift_wrapping_available":false,"gtin":null,"id":"874","images":[{"alt":"CTCF CUTANA CUTandRUN Antibody","data":"https://cdn11.bigcommerce.com/s-y9o92/images/stencil/{:size}/products/874/968/ctcf-cutana-cutandrun-antibody__00748.1645734560.jpg?c=2"}],"main_image":{"alt":"CTCF CUTANA CUTandRUN Antibody","data":"https://cdn11.bigcommerce.com/s-y9o92/images/stencil/{:size}/products/874/968/ctcf-cutana-cutandrun-antibody__00748.1645734560.jpg?c=2"},"max_purchase_quantity":0,"meta_description":"Rabbit monoclonal CTCF antibody validated for CUT&RUN, WB, IP, IHC, ICC. Rigorously tested for reliable and robust performance in CUT&RUN assays.","meta_keywords":"CTCF antibody, CCCTC-binding factor antibody, CUT&RUN antibody","min_purchase_quantity":0,"mpn":null,"num_reviews":0,"options":[],"page_title":"CTCF Antibody | CUTANA™ CUT&RUN Compatible","price":{"tax_label":"Sales Tax","without_tax":{"currency":"USD","formatted":"$445.00","value":445}},"rating":0,"related_products":[{"add_to_cart_url":"https://www.epicypher.com/cart.php?action=add&product_id=798","add_to_wishlist_url":"/wishlist.php?action=add&product_id=798","availability":"","brand":{"name":null},"category":["Antibodies","Antibodies/CUTANA™ CUT&RUN Antibodies","Antibodies/CUTANA™ CUT&RUN Antibodies/CUT&RUN Antibodies - Chromatin-Associated Proteins","Epigenetics Kits and Reagents/CUTANA™ ChIC / CUT&RUN Assays"],"custom_fields":[{"id":655,"name":"Pack size","value":"50 µL"}],"date_added":"25th Nov 2020","demo":false,"has_options":false,"id":798,"image":{"alt":"BRD4 CUTANA CUTandRUN Antibody","data":"https://cdn11.bigcommerce.com/s-y9o92/images/stencil/{:size}/products/798/933/brd4-cutana-cutandrun-antibody__13949.1645734524.jpg?c=2"},"images":[{"alt":"BRD4 CUTANA CUTandRUN Antibody","data":"https://cdn11.bigcommerce.com/s-y9o92/images/stencil/{:size}/products/798/933/brd4-cutana-cutandrun-antibody__13949.1645734524.jpg?c=2"}],"low_stock_level":null,"name":"BRD4 CUTANA™ CUT&RUN Antibody","num_reviews":null,"pre_order":false,"price":{"tax_label":"Sales Tax","without_tax":{"currency":"USD","formatted":"$445.00","value":445}},"qty_in_cart":0,"rating":null,"show_cart_action":true,"sku":"13-2003","stock_level":null,"summary":"\n \n \n Type: Polyclonal\n \n \n Host: Rabbit\n \n \n Target Size: 152 kD","url":"https://www.epicypher.com/products/antibodies/brd4-cutana-cut-run-antibody","weight":{"formatted":"0.01 LBS","value":0.01}},{"add_to_cart_url":"https://www.epicypher.com/cart.php?action=add&product_id=804","add_to_wishlist_url":"/wishlist.php?action=add&product_id=804","availability":"","brand":{"name":null},"category":["Antibodies","Antibodies/CUTANA™ CUT&RUN Antibodies","Antibodies/CUTANA™ CUT&RUN Antibodies/CUT&RUN Antibodies - 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- Type: Monoclonal [BLR041F]
- Host: Rabbit
- Target Size: 83 kDa
- Format: Purified IgG
- Reactivity: Human, Mouse
- Applications: CUT&RUN, WB, IP, IHC, ICC
Description
This CTCF antibody meets EpiCypher’s “CUTANA Compatible” criteria for performance in Cleavage Under Targets and Release Using Nuclease (CUT&RUN) and/or Cleavage Under Targets and Tagmentation (CUT&Tag) approaches to genomic mapping. Every lot of a CUTANA Compatible antibody is tested in the indicated CUTANA approach using EpiCypher optimized protocols and determined to yield peaks that show a genomic distribution pattern consistent with reported function(s) of the target protein. CTCF antibody produces CUT&RUN peaks at promoters (Figure 1A) and to a greater extent within intergenic and intronic regions (Figure 1B). CUT&RUN peaks overlap with known CTCF transcription factor binding motifs (Figure 2).
Validation Data
Figure 1: CTCF signal enrichment in CUT&RUN
CUT&RUN was performed using 500,000 K562 cells with CTCF
antibody (0.125 µg) and IgG control (0.5 µg, EpiCypher
13-0042). (A) Sequencing reads were aligned
to annotated TSSs (+/- 2 kbp) of 18,793 genes. High, medium,
and low signal is ranked by intensity (top to bottom;
aligned relative to CTCF) and reflected by red, yellow, and
blue colors, respectively. (B) CTCF peaks
were called using SEACR. The number of CTCF peaks which fall
into distinct classes of functionally annotated genomic
regions is plotted.
Figure 2: CTCF transcription factor binding motif
analysis in CUT&RUN
(A) MEME analysis determined that the CTCF
consensus motif, represented as a sequence logo position
weight matrix, was enriched under CUT&RUN peaks produced by CTCF
antibody. (B) The number of CTCF peaks containing
consensus motifs from panel A is represented by a Venn
Diagram. (C) Three representative loci
showing CTCF peaks (IGV).
Figure 3: Western blot detection of human and mouse
CTCF
Whole cell lysates were isolated from HEK293T (293T), MCF-7,
Hep-G2, A-549, SW620, SK-MEL-28 (SK), Jurkat, TCMK-1, and
NIH 3T3 cells using NETN lysis buffer. Fifty micrograms (50
µg) of each lysate was loaded onto an SDS-PAGE gel and
analyzed under standard western blot conditions using CTCF
antibody (1;1,000).
Figure 4: Immunoprecipitation of human CTCF
EpiCypher CTCF antibody (20 µL) was used to
immunoprecipitate whole cell lysates isolated from HEK293T
cells using NETN lysis buffer (1 mg per IP). A negative
control IgG antibody and a different CTCF positive control
antibody (Bethyl Laboratories) were also used to demonstrate
specificity of the IP. Immunoprecipitates were loaded onto
an SDS-PAGE gel (20% of IP loaded) and probed via western
blot with EpiCypher CTCF antibody (1:1,000).
Figure 5: Immunohistochemistry detection of human
CTCF
FFPE section of human lung carcinoma examined using CTCF
antibody (1:100 dilution).
Figure 6: Immunocytochemistry detection of human
CTCF
FFPE section of human MCF-7 cells examined using CTCF
antibody (1:100 dilution).
Technical Information
Application Notes
Recommended Dilutions:
CUT&RUN: 0.1 - 0.3 µg
Western Blot (WB): 1:1,000
Immunoprecipitation (IP): 20 µL/mg lysate
Immunohistochemistry (IHC): 1:100 - 1:500*
Immunocytochemistry (ICC): 1:100 - 1:500*
*Epitope retrieval with citrate buffer pH 6.0 recommended for FFPE tissues.
Documents & Resources
Additional Info
This CTCF antibody is provided for commercial sale under license from Bethyl Laboratories, Inc.