CUTANA™ Rabbit IgG CUT&RUN Negative Control Antibody

$50.00
SKU: 13-0042
Pack Size: 100 µg
Type:  Isotype Control Host:  Rabbit
Target Size:  N/A Appl.:  CUT&RUN
Format:  Aff. Pur. IgG Reactivity: Negative Control
CUTANA™ Rabbit IgG CUT&RUN Negative Control Antibody Description:
This Rabbit IgG Antibody can be used to control for non-specific background signal in CUT&RUN and other applications. A positive control antibody is also available (Histone H3K4me3 CUT&RUN Positive Control Antibody, EpiCypher Catalog No. 13-0041).

CUTANA™ Rabbit IgG CUT&RUN Negative Control Antibody Immunogen:
None.

CUTANA™ Rabbit IgG CUT&RUN Negative Control Antibody Formulation:
Protein A affinity-purified antibody in PBS, pH 7.6.

CUTANA™ Rabbit IgG CUT&RUN Negative Control Antibody Storage and Stability:
Stable for 1 year at 4°C from date of receipt.

CUTANA™ Rabbit IgG CUT&RUN Negative Control Antibody Application Notes Prior to use, prepare a 1:10 working stock dilution in CUT&RUN Antibody Buffer (20 mM HEPES pH 7.5, 150 mM NaCl, 0.5 mM Spermidine, 0.01% Digitonin, 2 mM EDTA). Store at 4°C for 1 month.
Recommended Dilution
For each CUT&RUN assay, dilute 1:100 from the working stock. A typical yield for a CUT&RUN assay using 500,000 K562 cells is 2-5 ng DNA.

CUTANA™ Rabbit IgG CUT&RUN Negative Control Antibody References:

View technical datasheet for this product. 13-0042 Datasheet

Applications Key: ChIP-Chromatin IP; E-ELISA; FACS-Flow cytometry; IF-Immunofluorescence; IHC-Immunohistochemistry; ICC-Immunocytochemistry; L-Luminex; IP-Immunoprecipitation; WB-Western Blotting

Reactivity Key: B-Bovine; Ce-C. elegans; Ch-Chicken; Dm- Drosophila; Eu-Eukaryote; H-Human; M-Mouse; Ma-Mammal; R-Rat; Sc-S.cerevesiae; Sp-S. pombe; WR-Wide Range (predicted); X-Xenopus; Z-Zebrafish

13-0042 Heat Map

Figure 1. CUT&RUN data was generated using the EpiCypher CUTANA™ CUT&RUN protocol (epicypher.com/cutana-protocol). Shown is a heatmap for Rabbit IgG CUT&RUN negative control antibody (left) compares to Histone H3K4me3 CUT&RUN positive control antibody (right, EpiCypher Catalog No. 13-0041). Signal from 2.2 and 3.9 million paired-end reads, repectively, are aligned to the transcription start site (TSS, +/- 2kb) of 18,793 genes. High and low signal are ranked by intensity (top to bottom) and reflected by red and blue colors, respectively.
(Click image to enlarge)

13-0042 CUT&RUN Data

Figure 2. Rabbit IgG (top track) and H3K4me3 (bottom track) CUT&RUN data were generated as described in Figure 1. Two representative loci are shown in the Integrative Genomics Viewer (IGV, Broad Institute). Window size is denoted at the top of each panel.
(Click image to enlarge)


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