

MLL1/KMT2A CUTANA™ CUT&RUN Antibody
{"AddThisServiceButtonMeta":"","add_this":[{"annotation":"","service":"facebook"},{"annotation":"","service":"email"},{"annotation":"","service":"print"},{"annotation":"","service":"twitter"},{"annotation":"","service":"linkedin"}],"add_to_wishlist_url":"/wishlist.php?action=add&product_id=801","availability":"","bulk_discount_rates":[],"can_purchase":true,"cart_url":"https://www.epicypher.com/cart.php","category":["Antibodies","Antibodies/CUTANA™ CUT&RUN Antibodies","Antibodies/CUTANA™ CUT&RUN Antibodies/CUT&RUN Antibodies - Chromatin-Associated Proteins","Epigenetics Kits and Reagents/CUTANA™ ChIC / CUT&RUN Assays"],"custom_fields":[{"id":"663","name":"Pack Size","value":"100 µL"}],"customizations":[],"description":"<div class=\"product-general-info\">\n <ul class=\"product-general-info__list-left\">\n <li class=\"product-general-info__list-item\">\n <strong>Type: </strong>Monoclonal [BL-175-7E8]\n </li>\n <li class=\"product-general-info__list-item\">\n <strong>Host: </strong>Rabbit\n </li>\n <li class=\"product-general-info__list-item\">\n <strong>Target Size: </strong>431 kDa\n </li>\n </ul>\n <ul class=\"product-general-info__list-right\">\n <li class=\"product-general-info__list-item\">\n <strong>Format: </strong>Purified IgG\n </li>\n <li class=\"product-general-info__list-item\">\n <strong>Reactivity: </strong>Human\n </li>\n <li class=\"product-general-info__list-item\">\n <strong>Applications: </strong>CUT&RUN, WB, IP\n </li>\n </ul>\n</div>\n<div class=\"service_accordion product-droppdown\">\n <div class=\"container\">\n <div id=\"prodAccordion\">\n <div id=\"ProductDescription\" class=\"Block Panel current\">\n <h3 class=\"sub-title1\">Description</h3>\n <div\n class=\"\n ProductDescriptionContainer\n product-droppdown__section-description-specific\n \"\n >\n <p>\n This antibody meets EpiCypher’s “CUTANA Compatible” criteria for\n performance in Cleavage Under Targets and Release Using Nuclease\n (CUT&RUN) and/or Cleavage Under Targets and Tagmentation\n (CUT&Tag) approaches to genomic mapping. Every lot of a CUTANA\n Compatible antibody is tested in the indicated CUTANA approach using\n <a href=\"/resources/protocols\">EpiCypher optimized protocols </a>and\n determined to yield peaks that show a genomic distribution pattern\n consistent with reported function(s) of the target protein. MLL1\n antibody produces CUT&RUN peaks above background\n <strong>(Figure 1)</strong> that overlap with H3K4me3\n <strong>(Figures 1-2)</strong>, consistent with its known role as\n the catalytic subunit of the MLL1/MLL histone methyltransferase\n complex.\n </p>\n </div>\n </div>\n </div>\n <div id=\"prodAccordion\">\n <div id=\"ProductDescription\" class=\"Block Panel current\">\n <h3 class=\"sub-title1\">Validation Data</h3>\n <div\n class=\"\n ProductDescriptionContainer\n product-droppdown__section-description-specific\n \"\n >\n <section class=\"image-picker\">\n <div class=\"image-picker__left\">\n <div\n class=\"\n image-picker__main-content_active image-picker__main-content\n \"\n >\n <div class=\"image-picker__header-content\">\n <button class=\"image-picker__left-arrow\">\n <svg\n class=\"image-picker__svg-left\"\n width=\"24\"\n height=\"24\"\n viewBox=\"0 0 24 24\"\n >\n <path\n d=\"M16.67 0l2.83 2.829-9.339 9.175 9.339 9.167-2.83 2.829-12.17-11.996z\"\n />\n </svg>\n </button>\n <a\n href=\"/content/images/products/antibodies/13_2004_Heatmap.jpg\"\n target=\"_new\"\n class=\"image-picker__main-image-link\"\n ><img\n alt=\"13_2004_Heatmap\"\n src=\"/content/images/products/antibodies/13_2004_Heatmap.jpg\"\n class=\"image-picker__main-image\"\n />\n <span class=\"image-picker__main-image-caption\"\n >(Click to enlarge)</span\n ></a\n >\n <button class=\"image-picker__right-arrow\">\n <svg\n class=\"image-picker__svg-right\"\n width=\"24\"\n height=\"24\"\n viewBox=\"0 0 24 24\"\n >\n <path\n d=\"M7.33 24l-2.83-2.829 9.339-9.175-9.339-9.167 2.83-2.829 12.17 11.996z\"\n />\n </svg>\n </button>\n </div>\n <p>\n <span class=\"image-picker__span-content\"\n ><strong\n >Figure 1: MLL1 enrichment at annotated transcription\n start sites (TSSs) in CUT&RUN </strong\n ><br />\n CUT&RUN was performed using 500,000 K562 cells with MLL1 and\n control antibodies (0.5 µg each; IgG negative control,\n EpiCypher\n <a\n href=\"/products/epigenetics-reagents-and-assays/cutana-chic-cut-run-assays/cutana-rabbit-igg-cut-run-negative-control-antibody\"\n >13-0042</a\n >; H3K4me3 positive control, EpiCypher\n <a\n href=\"/products/antibodies/snap-chip-certified-antibodies/histone-h3k4me3-antibody-snap-chip-certified-cutana-cut-run-compatible\"\n >13-0041</a\n >). Sequencing reads were aligned to annotated TSSs (+/- 2\n kbp) of 18,793 genes. High, medium, and low signal is ranked\n by intensity (top to bottom) and reflected by red, yellow,\n and blue colors, respectively. All rows aligned relative to\n MLL1.\n </span>\n </p>\n </div>\n <div class=\"image-picker__main-content\">\n <div class=\"image-picker__header-content\">\n <button class=\"image-picker__left-arrow\">\n <svg\n class=\"image-picker__svg-left\"\n width=\"24\"\n height=\"24\"\n viewBox=\"0 0 24 24\"\n >\n <path\n d=\"M16.67 0l2.83 2.829-9.339 9.175 9.339 9.167-2.83 2.829-12.17-11.996z\"\n />\n </svg>\n </button>\n <a\n href=\"/content/images/products/antibodies/13_2004_Tracks.jpg\"\n target=\"_new\"\n class=\"image-picker__main-image-link\"\n ><img\n alt=\"13_2004_tracks\"\n src=\"/content/images/products/antibodies/13_2004_Tracks.jpg\"\n class=\"image-picker__main-image\"\n />\n <span class=\"image-picker__main-image-caption\"\n >(Click to enlarge)</span\n ></a\n >\n <button class=\"image-picker__right-arrow\">\n <svg\n class=\"image-picker__svg-right\"\n width=\"24\"\n height=\"24\"\n viewBox=\"0 0 24 24\"\n >\n <path\n d=\"M7.33 24l-2.83-2.829 9.339-9.175-9.339-9.167 2.83-2.829 12.17 11.996z\"\n />\n </svg>\n </button>\n </div>\n <p>\n <span class=\"image-picker__span-content\"\n ><strong\n >Figure 2: MLL1 CUT&RUN peaks and functional\n overlap</strong\n ><br />\n Two representative gene loci from the CUT&RUN data in Figure\n 1 are shown. MLL1 peaks overlap with H3K4me3 CUT&RUN peaks\n (EpiCypher 13-0041), consistent with the reported function\n of MLL1 as an H3K4 histone methyltransferase. Images were\n generated using the Integrative Genomics Viewer (Broad\n Institute).\n </span>\n </p>\n </div>\n <div class=\"image-picker__main-content\">\n <div class=\"image-picker__header-content\">\n <button class=\"image-picker__left-arrow\">\n <svg\n class=\"image-picker__svg-left\"\n width=\"24\"\n height=\"24\"\n viewBox=\"0 0 24 24\"\n >\n <path\n d=\"M16.67 0l2.83 2.829-9.339 9.175 9.339 9.167-2.83 2.829-12.17-11.996z\"\n />\n </svg>\n </button>\n <a\n href=\"/content/images/products/antibodies/13_2004_WB.jpg\"\n target=\"_new\"\n class=\"image-picker__main-image-link\"\n ><img\n alt=\"13_2004_WB_H\"\n src=\"/content/images/products/antibodies/13_2004_WB.jpg\"\n class=\"image-picker__main-image\"\n />\n <span class=\"image-picker__main-image-caption\"\n >(Click to enlarge)</span\n ></a\n >\n <button class=\"image-picker__right-arrow\">\n <svg\n class=\"image-picker__svg-right\"\n width=\"24\"\n height=\"24\"\n viewBox=\"0 0 24 24\"\n >\n <path\n d=\"M7.33 24l-2.83-2.829 9.339-9.175-9.339-9.167 2.83-2.829 12.17 11.996z\"\n />\n </svg>\n </button>\n </div>\n <p>\n <span class=\"image-picker__span-content\"\n ><strong\n >Figure 3: Western blot detection of human MLL1</strong\n ><br />\n Whole cell lysates were isolated from HEK293T, HeLa, MCF-7,\n HEP-G2, A-549, and SW620 cells using NETN lysis buffer.\n Fifty micrograms (50 µg) of lysate were loaded onto SDS-PAGE\n gel and analyzed under standard western blot conditions\n using MLL1 antibody (1:1,000 dilution).\n </span>\n </p>\n </div>\n <div class=\"image-picker__main-content\">\n <div class=\"image-picker__header-content\">\n <button class=\"image-picker__left-arrow\">\n <svg\n class=\"image-picker__svg-left\"\n width=\"24\"\n height=\"24\"\n viewBox=\"0 0 24 24\"\n >\n <path\n d=\"M16.67 0l2.83 2.829-9.339 9.175 9.339 9.167-2.83 2.829-12.17-11.996z\"\n />\n </svg>\n </button>\n <a\n href=\"/content/images/products/antibodies/13_2004_IP.jpg\"\n target=\"_new\"\n class=\"image-picker__main-image-link\"\n ><img\n alt=\"13_2004_WB_M\"\n src=\"/content/images/products/antibodies/13_2004_IP.jpg\"\n class=\"image-picker__main-image\"\n />\n <span class=\"image-picker__main-image-caption\"\n >(Click to enlarge)</span\n ></a\n >\n <button class=\"image-picker__right-arrow\">\n <svg\n class=\"image-picker__svg-right\"\n width=\"24\"\n height=\"24\"\n viewBox=\"0 0 24 24\"\n >\n <path\n d=\"M7.33 24l-2.83-2.829 9.339-9.175-9.339-9.167 2.83-2.829 12.17 11.996z\"\n />\n </svg>\n </button>\n </div>\n <p>\n <span class=\"image-picker__span-content\"\n ><strong>Figure 4: Immunoprecipitation of human MLL1</strong\n ><br />\n EpiCypher MLL1 antibody (10 µL) was used to\n immunoprecipitate whole cell lysates isolated from HEK293T\n cells using NETN lysis buffer (0.5 mg per IP). A negative\n control IgG antibody and positive control antibodies to\n various MLL1 epitopes (Bethyl Laboratories) were also used\n to demonstrate specificity of the IP. Immunoprecipitates\n were loaded onto SDS-PAGE gel (20% of IP loaded) and probed\n via western blot with EpiCypher MLL1 antibody (1:1,000\n dilution).\n </span>\n </p>\n </div>\n </div>\n <aside class=\"image-picker__right\">\n <div class=\"image-picker__gallery\">\n <img\n alt=\"13_2004_Heatmap\"\n src=\"/content/images/products/antibodies/13_2004_Heatmap.jpg\"\n width=\"200\"\n class=\"\n image-picker__side-image image-picker__side-image_active\n \"\n role=\"button\"\n />\n <img\n alt=\"13_2004_tracks\"\n src=\"/content/images/products/antibodies/13_2004_Tracks.jpg\"\n class=\"image-picker__side-image\"\n role=\"button\"\n />\n <img\n alt=\"13_2004_WB_H\"\n src=\"/content/images/products/antibodies/13_2004_WB.jpg\"\n class=\"image-picker__side-image\"\n role=\"button\"\n />\n <img\n alt=\"13_2004_WB_M\"\n src=\"/content/images/products/antibodies/13_2004_IP.jpg\"\n class=\"image-picker__side-image\"\n role=\"button\"\n />\n </div>\n </aside>\n </section>\n </div>\n </div>\n </div>\n <div id=\"prodAccordion\">\n <div id=\"ProductDescription\" class=\"Block Panel\">\n <h3 class=\"sub-title1\">Technical Information</h3>\n <div\n class=\"\n ProductDescriptionContainer\n product-droppdown__section-description\n \"\n >\n <div class=\"product-tech-info\">\n <div class=\"product-tech-info__line-item\">\n <div class=\"product-tech-info__line-item-left\">\n <b>Immunogen</b>\n </div>\n <div class=\"product-tech-info__line-item-right\">\n A synthetic peptide corresponding to human MLL1 amino acids 720\n to 780.\n </div>\n </div>\n <div class=\"product-tech-info__line-item\">\n <div class=\"product-tech-info__line-item-left\">\n <b>Formulation</b>\n </div>\n <div class=\"product-tech-info__line-item-right\">\n Purified recombinant monoclonal antibody (1.0 mg/mL) in borate\n buffered saline (BBS) pH 8.2 with 0.09% sodium azide.\n </div>\n </div>\n <div class=\"product-tech-info__line-item\">\n <div class=\"product-tech-info__line-item-left\">\n <b>Storage and Stability</b>\n </div>\n <div class=\"product-tech-info__line-item-right\">\n Stable for 1 year at 4°C from date of receipt.\n </div>\n </div>\n </div>\n </div>\n </div>\n </div>\n <div id=\"prodAccordion\">\n <div id=\"ProductDescription\" class=\"Block Panel\">\n <h3 class=\"sub-title1\">Application Notes</h3>\n <div\n class=\"\n ProductDescriptionContainer\n product-droppdown__section-description\n \"\n >\n <p><strong>Recommended Dilutions:</strong></p>\n <p><strong>CUT&RUN:</strong> 0.5 µg</p>\n <p><strong>Western Blot (WB):</strong> 1:1,000</p>\n <p>\n <strong>Immunoprecipitation (IP):</strong> 10 µL / 0.5 mg lysate\n </p>\n </div>\n </div>\n </div>\n\n <div id=\"prodAccordion\">\n <div id=\"ProductDescription\" class=\"Block Panel\">\n <h3 class=\"sub-title1\">Documents & Resources</h3>\n <div\n class=\"\n ProductDescriptionContainer\n product-droppdown__section-description\n \"\n >\n <div class=\"product-documents\">\n <a\n href=\"/content/documents/tds/13-2004.pdf\"\n target=\"_new\"\n class=\"product-documents__link\"\n >\n <svg\n version=\"1.1\"\n id=\"Layer_1\"\n xmlns=\"http://www.w3.org/2000/svg\"\n xmlns:xlink=\"http://www.w3.org/1999/xlink\"\n x=\"0px\"\n y=\"0px\"\n viewBox=\"0 0 228 240\"\n style=\"enable-background: new 0 0 228 240\"\n xml:space=\"preserve\"\n class=\"product-documents__icon\"\n >\n <g>\n <path\n class=\"product-documents__svg-pdf\"\n d=\"M191.92,68.77l-47.69-47.69c-1.33-1.33-3.12-2.08-5.01-2.08H45.09C41.17,19,38,22.17,38,26.09v184.36\n c0,3.92,3.17,7.09,7.09,7.09h141.82c3.92,0,7.09-3.17,7.09-7.09V73.8C194,71.92,193.25,70.1,191.92,68.77z M177.65,77.06h-41.7\n v-41.7L177.65,77.06z M178.05,201.59H53.95V34.95h66.92v47.86c0,5.14,4.17,9.31,9.31,9.31h47.86V201.59z\"\n />\n </g>\n <rect\n x=\"20\"\n y=\"112\"\n class=\"product-documents__svg-background\"\n width=\"146\"\n height=\"76\"\n />\n <g>\n <path\n class=\"product-documents__svg-pdf\"\n d=\"M23.83,125.68h22.36c5.29,0,9.41,1.33,12.35,4c2.94,2.67,4.42,6.39,4.42,11.18c0,4.78-1.47,8.51-4.42,11.18\n c-2.94,2.67-7.06,4-12.35,4H34.59v18.29H23.83V125.68z M44.81,147.9c5.38,0,8.07-2.32,8.07-6.97c0-2.39-0.67-4.16-2-5.31\n c-1.33-1.15-3.36-1.73-6.07-1.73H34.59v14.01H44.81z\"\n />\n <path\n class=\"product-documents__svg-pdf\"\n d=\"M69.92,125.68h18.91c5.29,0,9.84,0.97,13.66,2.9c3.82,1.93,6.74,4.72,8.76,8.35\n c2.02,3.63,3.04,7.98,3.04,13.04c0,5.06-1,9.42-3,13.08c-2,3.66-4.91,6.45-8.73,8.38c-3.82,1.93-8.4,2.9-13.73,2.9H69.92V125.68z\n M88.07,165.63c10.35,0,15.52-5.22,15.52-15.66c0-10.4-5.17-15.59-15.52-15.59h-7.38v31.26H88.07z\"\n />\n <path\n class=\"product-documents__svg-pdf\"\n d=\"M122.57,125.68h32.84v8.49h-22.22v11.18h20.84v8.49h-20.84v20.49h-10.63V125.68z\"\n />\n </g>\n </svg>\n <span class=\"product-documents__info\">Technical Datasheet</span>\n </a>\n </div>\n </div>\n </div>\n </div>\n <div id=\"prodAccordion\">\n <div id=\"ProductDescription\" class=\"Block Panel\">\n <h3 class=\"sub-title1\">Additional Info</h3>\n <div\n class=\"\n ProductDescriptionContainer\n product-droppdown__section-description\n \"\n >\n <p>\n This product is provided for commercial sale under license from\n Bethyl Laboratories, Inc.\n </p>\n\n <strong>Applications Key:</strong> <br /><br />\n\n ChIP: Chromatin immunoprecipitation<br />\n CUT&RUN: Cleavage Under Targets and Release Using Nuclease<br />\n CUT&Tag: Cleavage Under Targets and Tagmentation<br />\n E: ELISA<br />\n FACS: Flow cytometry<br />\n ICC: Immunocytochemistry<br />\n IF: Immunofluorescence<br />\n IHC: Immunohistochemistry<br />\n IP: Immunoprecipitation<br />\n L: Luminex<br />\n WB: Western Blot<br /><br />\n\n <strong>Reactivity Key:</strong> <br /><br />\n\n B: Bovine<br />\n Ce: <em>C. elegans</em><br />\n Ch: Chicken<br />\n Dm: <em>Drosophila</em><br />\n Eu: Eukaryote<br />\n H: Human<br />\n M: Mouse<br />\n Ma: Mammal<br />\n R: Rat<br />\n Sc: <em>S. cerevisiae</em><br />\n Sp: <em>S. pombe</em><br />\n WR: Wide Range (predicted)<br />\n X: Xenopus<br />\n Z: Zebrafish<br />\n </div>\n </div>\n </div>\n </div>\n</div>\n","detail_messages":"","gift_wrapping_available":false,"gtin":null,"id":"801","images":[{"alt":"MLL1/KMT2A CUTANA™ CUT&RUN Antibody","data":"https://cdn11.bigcommerce.com/s-y9o92/images/stencil/{:size}/products/801/790/2020.12.08_CUTANA_ChAPs_Antibody_thumbnail_RGB_LS__68774.1614276469.png?c=2"}],"main_image":{"alt":"MLL1/KMT2A CUTANA™ CUT&RUN Antibody","data":"https://cdn11.bigcommerce.com/s-y9o92/images/stencil/{:size}/products/801/790/2020.12.08_CUTANA_ChAPs_Antibody_thumbnail_RGB_LS__68774.1614276469.png?c=2"},"max_purchase_quantity":0,"meta_description":"Recombinant monoclonal anti-MLL1/KMT2A antibody validated for CUT&RUN, WB, IP. Rigorously tested for reliable and robust performance in CUT&RUN assays.","meta_keywords":"mll1 antibody, kmt2a antibody, CUT&RUN antibody","min_purchase_quantity":0,"mpn":null,"num_reviews":0,"options":[],"page_title":"MLL1/KMT2A Antibody | CUTANA™ CUT&RUN Compatible","price":{"tax_label":"Sales Tax","without_tax":{"currency":"USD","formatted":"$445.00","value":445}},"rating":0,"related_products":[{"add_to_cart_url":"https://www.epicypher.com/cart.php?action=add&product_id=798","add_to_wishlist_url":"/wishlist.php?action=add&product_id=798","availability":"","brand":{"name":null},"category":["Antibodies","Antibodies/CUTANA™ CUT&RUN Antibodies","Antibodies/CUTANA™ CUT&RUN Antibodies/CUT&RUN Antibodies - Chromatin-Associated Proteins","Epigenetics Kits and Reagents/CUTANA™ ChIC / CUT&RUN Assays"],"custom_fields":[{"id":655,"name":"Pack size","value":"50 µL"}],"date_added":"25th Nov 2020","demo":false,"has_options":false,"id":798,"image":{"alt":"BRD4 CUTANA CUTandRUN Antibody","data":"https://cdn11.bigcommerce.com/s-y9o92/images/stencil/{:size}/products/798/933/brd4-cutana-cutandrun-antibody__13949.1645734524.jpg?c=2"},"images":[{"alt":"BRD4 CUTANA CUTandRUN Antibody","data":"https://cdn11.bigcommerce.com/s-y9o92/images/stencil/{:size}/products/798/933/brd4-cutana-cutandrun-antibody__13949.1645734524.jpg?c=2"}],"low_stock_level":null,"name":"BRD4 CUTANA™ CUT&RUN Antibody","num_reviews":null,"pre_order":false,"price":{"tax_label":"Sales Tax","without_tax":{"currency":"USD","formatted":"$445.00","value":445}},"qty_in_cart":0,"rating":null,"show_cart_action":true,"sku":"13-2003","stock_level":null,"summary":"\n \n \n Type: Polyclonal\n \n \n Host: Rabbit\n \n \n Target Size: 152 kD","url":"https://www.epicypher.com/products/antibodies/brd4-cutana-cut-run-antibody","weight":{"formatted":"0.01 LBS","value":0.01}},{"add_to_cart_url":"https://www.epicypher.com/cart.php?action=add&product_id=804","add_to_wishlist_url":"/wishlist.php?action=add&product_id=804","availability":"","brand":{"name":null},"category":["Antibodies","Antibodies/CUTANA™ CUT&RUN Antibodies","Antibodies/CUTANA™ CUT&RUN Antibodies/CUT&RUN Antibodies - 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- Type: Monoclonal [BL-175-7E8]
- Host: Rabbit
- Target Size: 431 kDa
- Format: Purified IgG
- Reactivity: Human
- Applications: CUT&RUN, WB, IP
Description
This antibody meets EpiCypher’s “CUTANA Compatible” criteria for performance in Cleavage Under Targets and Release Using Nuclease (CUT&RUN) and/or Cleavage Under Targets and Tagmentation (CUT&Tag) approaches to genomic mapping. Every lot of a CUTANA Compatible antibody is tested in the indicated CUTANA approach using EpiCypher optimized protocols and determined to yield peaks that show a genomic distribution pattern consistent with reported function(s) of the target protein. MLL1 antibody produces CUT&RUN peaks above background (Figure 1) that overlap with H3K4me3 (Figures 1-2), consistent with its known role as the catalytic subunit of the MLL1/MLL histone methyltransferase complex.
Validation Data
Figure 1: MLL1 enrichment at annotated transcription
start sites (TSSs) in CUT&RUN
CUT&RUN was performed using 500,000 K562 cells with MLL1 and
control antibodies (0.5 µg each; IgG negative control,
EpiCypher
13-0042; H3K4me3 positive control, EpiCypher
13-0041). Sequencing reads were aligned to annotated TSSs (+/- 2
kbp) of 18,793 genes. High, medium, and low signal is ranked
by intensity (top to bottom) and reflected by red, yellow,
and blue colors, respectively. All rows aligned relative to
MLL1.
Figure 2: MLL1 CUT&RUN peaks and functional
overlap
Two representative gene loci from the CUT&RUN data in Figure
1 are shown. MLL1 peaks overlap with H3K4me3 CUT&RUN peaks
(EpiCypher 13-0041), consistent with the reported function
of MLL1 as an H3K4 histone methyltransferase. Images were
generated using the Integrative Genomics Viewer (Broad
Institute).
Figure 3: Western blot detection of human MLL1
Whole cell lysates were isolated from HEK293T, HeLa, MCF-7,
HEP-G2, A-549, and SW620 cells using NETN lysis buffer.
Fifty micrograms (50 µg) of lysate were loaded onto SDS-PAGE
gel and analyzed under standard western blot conditions
using MLL1 antibody (1:1,000 dilution).
Figure 4: Immunoprecipitation of human MLL1
EpiCypher MLL1 antibody (10 µL) was used to
immunoprecipitate whole cell lysates isolated from HEK293T
cells using NETN lysis buffer (0.5 mg per IP). A negative
control IgG antibody and positive control antibodies to
various MLL1 epitopes (Bethyl Laboratories) were also used
to demonstrate specificity of the IP. Immunoprecipitates
were loaded onto SDS-PAGE gel (20% of IP loaded) and probed
via western blot with EpiCypher MLL1 antibody (1:1,000
dilution).
Technical Information
Application Notes
Recommended Dilutions:
CUT&RUN: 0.5 µg
Western Blot (WB): 1:1,000
Immunoprecipitation (IP): 10 µL / 0.5 mg lysate
Documents & Resources
Additional Info
This product is provided for commercial sale under license from Bethyl Laboratories, Inc.
Applications Key:ChIP: Chromatin immunoprecipitation
CUT&RUN: Cleavage Under Targets and Release Using Nuclease
CUT&Tag: Cleavage Under Targets and Tagmentation
E: ELISA
FACS: Flow cytometry
ICC: Immunocytochemistry
IF: Immunofluorescence
IHC: Immunohistochemistry
IP: Immunoprecipitation
L: Luminex
WB: Western Blot
Reactivity Key:
B: Bovine
Ce: C. elegans
Ch: Chicken
Dm: Drosophila
Eu: Eukaryote
H: Human
M: Mouse
Ma: Mammal
R: Rat
Sc: S. cerevisiae
Sp: S. pombe
WR: Wide Range (predicted)
X: Xenopus
Z: Zebrafish