MLL1/KMT2A CUTANA™ CUT&RUN Antibody
{"url":"https://www.epicypher.com/products/antibodies/mll1-kmt2a-cutana-cut-run-antibody","add_this":[{"service":"facebook","annotation":""},{"service":"email","annotation":""},{"service":"print","annotation":""},{"service":"twitter","annotation":""},{"service":"linkedin","annotation":""}],"gtin":null,"id":"801","bulk_discount_rates":[],"can_purchase":true,"meta_description":"Recombinant monoclonal anti-MLL1/KMT2A antibody validated for CUT&RUN, WB, IP. Rigorously tested for reliable and robust performance in CUT&RUN assays.","category":["Antibodies","Antibodies/CUTANA™ CUT&RUN Antibodies","Antibodies/CUTANA™ CUT&RUN Antibodies/CUT&RUN Antibodies - Chromatin-Associated Proteins","Epigenetics Kits and Reagents/CUTANA™ ChIC / CUT&RUN Assays"],"AddThisServiceButtonMeta":"","main_image":{"data":"https://cdn11.bigcommerce.com/s-y9o92/images/stencil/{:size}/products/801/790/2020.12.08_CUTANA_ChAPs_Antibody_thumbnail_RGB_LS__68774.1614276469.png?c=2","alt":"MLL1/KMT2A CUTANA™ CUT&RUN Antibody"},"add_to_wishlist_url":"/wishlist.php?action=add&product_id=801","shipping":{"calculated":true},"num_reviews":0,"weight":"0.01 LBS","custom_fields":[{"id":"663","name":"Pack Size","value":"100 µL"}],"sku":"13-2004","description":"<div class=\"product-general-info\">\n <ul class=\"product-general-info__list-left\">\n <li class=\"product-general-info__list-item\">\n <strong>Type: </strong>Monoclonal\n </li>\n <li class=\"product-general-info__list-item\">\n <strong>Host: </strong>Rabbit\n </li>\n <li class=\"product-general-info__list-item\">\n <strong>Applications: </strong>CUT&RUN, IP, WB\n </li>\n </ul>\n <ul class=\"product-general-info__list-right\">\n <li class=\"product-general-info__list-item\">\n <strong>Reactivity: </strong>Human\n </li>\n <li class=\"product-general-info__list-item\">\n <strong>Format: </strong>Purified\n </li>\n <li class=\"product-general-info__list-item\">\n <strong>Target Size: </strong>431 kDa\n </li>\n </ul>\n <ul style=\"padding: 0\" class=\"product-general-info__list-right\">\n <li class=\"product-general-info__list-item\">\n <a href=\"#bioz\">\n <div\n id=\"w-s-3835-13-2004\"\n style=\"\n width: 240px;\n height: 58px;\n position: relative;\n overflow-y: hidden;\n \"\n ></div>\n <div id=\"bioz-w-pb-13-2004-div\" style=\"width: 240px\">\n <a\n id=\"bioz-w-pb-13-2004\"\n style=\"font-size: 12px; color: transparent\"\n href=\"https://www.bioz.com/\"\n target=\"_blank\"\n >\n <img\n src=\"https://cdn.bioz.com/assets/favicon.png\"\n style=\"\n width: 11px;\n height: 11px;\n vertical-align: baseline;\n padding-bottom: 0px;\n margin-left: 0px;\n margin-bottom: 0px;\n float: none;\n display: none;\n \"\n />\n Powered by Bioz\n </a>\n </div>\n </a>\n </li>\n </ul>\n</div>\n<div class=\"service_accordion product-droppdown\">\n <div class=\"container\">\n <div id=\"prodAccordion\">\n <div id=\"ProductDescription\" class=\"Block Panel current\">\n <h3 class=\"sub-title1\">Description</h3>\n <div\n class=\"ProductDescriptionContainer product-droppdown__section-description-specific\"\n >\n <p>\n This antibody meets EpiCypher’s “CUTANA Compatible” criteria\n for performance in Cleavage Under Targets and Release Using\n Nuclease (CUT&RUN) and/or Cleavage Under Targets and\n Tagmentation (CUT&Tag) approaches to genomic mapping.\n Every lot of a CUTANA Compatible antibody is tested in the\n indicated CUTANA approach using\n <a href=\"/resources/protocols\"\n >EpiCypher optimized protocols </a\n >and determined to yield peaks that show a genomic\n distribution pattern consistent with reported function(s) of\n the target protein. MLL1 antibody produces CUT&RUN peaks above\n background <strong>(Figure 1)</strong> that overlap with\n H3K4me3 <strong>(Figures 1-2)</strong>, consistent with its\n known role as the catalytic subunit of the MLL1/MLL histone\n methyltransferase complex.\n </p>\n </div>\n </div>\n </div>\n <div id=\"prodAccordion\">\n <div id=\"ProductDescription\" class=\"Block Panel current\">\n <h3 class=\"sub-title1\">Validation Data</h3>\n <div\n class=\"ProductDescriptionContainer product-droppdown__section-description-specific\"\n >\n <section class=\"image-picker\">\n <div class=\"image-picker__left\">\n <div\n class=\"image-picker__main-content_active image-picker__main-content\"\n >\n <div class=\"image-picker__header-content\">\n <button class=\"image-picker__left-arrow\">\n <svg\n class=\"image-picker__svg-left\"\n width=\"24\"\n height=\"24\"\n viewBox=\"0 0 24 24\"\n >\n <path\n d=\"M16.67 0l2.83 2.829-9.339 9.175 9.339 9.167-2.83 2.829-12.17-11.996z\"\n />\n </svg>\n </button>\n <a\n href=\"/content/images/products/antibodies/13-2004-mll1-peaks.jpeg\"\n target=\"_new\"\n class=\"image-picker__main-image-link\"\n ><img\n loading=\"lazy\"\n alt=\"13-2004-mll1-peaks\"\n src=\"/content/images/products/antibodies/13-2004-mll1-peaks.jpeg\"\n class=\"image-picker__main-image\"\n />\n <span class=\"image-picker__main-image-caption\"\n >(Click to enlarge)</span\n ></a\n >\n <button class=\"image-picker__right-arrow\">\n <svg\n class=\"image-picker__svg-right\"\n width=\"24\"\n height=\"24\"\n viewBox=\"0 0 24 24\"\n >\n <path\n d=\"M7.33 24l-2.83-2.829 9.339-9.175-9.339-9.167 2.83-2.829 12.17 11.996z\"\n />\n </svg>\n </button>\n </div>\n <p>\n <span class=\"image-picker__span-content\"\n ><strong>Figure 1: MLL1 peaks in CUT&RUN </strong\n ><br />\n CUT&RUN was performed as described in Figure 5. Peaks\n were called with MACS2. Heatmaps show MLL1 peaks\n relative to IgG negative control or H3K4me3 positive\n control antibodies in aligned rows ranked by intensity\n (top to bottom) and colored such that red indicates\n high localized enrichment and blue denotes background\n signal. MLL1 and H3K4me3 antibodies showed expected\n enrichment around the TSS. Despite some observable\n background in the IgG control, differential enrichment\n with the target antibodies is as expected.\n </span>\n </p>\n </div>\n <div class=\"image-picker__main-content\">\n <div class=\"image-picker__header-content\">\n <button class=\"image-picker__left-arrow\">\n <svg\n class=\"image-picker__svg-left\"\n width=\"24\"\n height=\"24\"\n viewBox=\"0 0 24 24\"\n >\n <path\n d=\"M16.67 0l2.83 2.829-9.339 9.175 9.339 9.167-2.83 2.829-12.17-11.996z\"\n />\n </svg>\n </button>\n <a\n href=\"/content/images/products/antibodies/13-2004-representative-browser-tracks.jpeg\"\n target=\"_new\"\n class=\"image-picker__main-image-link\"\n ><img\n loading=\"lazy\"\n alt=\"13-2004-representative-browser-tracks\"\n src=\"/content/images/products/antibodies/13-2004-representative-browser-tracks.jpeg\"\n class=\"image-picker__main-image\"\n />\n <span class=\"image-picker__main-image-caption\"\n >(Click to enlarge)</span\n ></a\n >\n <button class=\"image-picker__right-arrow\">\n <svg\n class=\"image-picker__svg-right\"\n width=\"24\"\n height=\"24\"\n viewBox=\"0 0 24 24\"\n >\n <path\n d=\"M7.33 24l-2.83-2.829 9.339-9.175-9.339-9.167 2.83-2.829 12.17 11.996z\"\n />\n </svg>\n </button>\n </div>\n <p>\n <span class=\"image-picker__span-content\"\n ><strong\n >Figure 2: MLL1 CUT&RUN representative browser\n tracks</strong\n ><br />\n CUT&RUN was performed as described in Figure 5. Gene\n browser shots were generated using the Integrative\n Genomics Viewer (IGV, Broad Institute). Two\n representative loci show overlap of MLL1 and H3K4me3\n peaks.\n </span>\n </p>\n </div>\n <div class=\"image-picker__main-content\">\n <div class=\"image-picker__header-content\">\n <button class=\"image-picker__left-arrow\">\n <svg\n class=\"image-picker__svg-left\"\n width=\"24\"\n height=\"24\"\n viewBox=\"0 0 24 24\"\n >\n <path\n d=\"M16.67 0l2.83 2.829-9.339 9.175 9.339 9.167-2.83 2.829-12.17-11.996z\"\n />\n </svg>\n </button>\n <a\n href=\"/content/images/products/antibodies/13-2004-immunoprecipitation.jpeg\"\n target=\"_new\"\n class=\"image-picker__main-image-link\"\n ><img\n loading=\"lazy\"\n alt=\"13-2004-immunoprecipitation\"\n src=\"/content/images/products/antibodies/13-2004-immunoprecipitation.jpeg\"\n class=\"image-picker__main-image\"\n />\n <span class=\"image-picker__main-image-caption\"\n >(Click to enlarge)</span\n ></a\n >\n <button class=\"image-picker__right-arrow\">\n <svg\n class=\"image-picker__svg-right\"\n width=\"24\"\n height=\"24\"\n viewBox=\"0 0 24 24\"\n >\n <path\n d=\"M7.33 24l-2.83-2.829 9.339-9.175-9.339-9.167 2.83-2.829 12.17 11.996z\"\n />\n </svg>\n </button>\n </div>\n <p>\n <span class=\"image-picker__span-content\"\n ><strong>Figure 3: Immunoprecipitation data</strong\n ><br />\n EpiCypher MLL1 antibody (6 µL/mg lysate) was used to\n immunoprecipitate whole cell lysates (1 mg per IP\n reaction; 20% of IP loaded) isolated from HEK293T\n cells using NETN lysis buffer. A negative control IgG\n antibody and positive control antibodies targeting\n MLL1 (Bethyl Laboratories) were also used to\n demonstrate specificity of the IP. EpiCypher 13-2004,\n Bethyl A700-010-1, and Bethyl A300-086-6 target the\n same epitope, while Bethyl A300-087-3 targets a\n different epitope (between amino acids 1320 and 1380)\n For blotting immunoprecipitates, EpiCypher MLL1\n antibody was used at a dilution of 1:1,000.\n </span>\n </p>\n </div>\n <div class=\"image-picker__main-content\">\n <div class=\"image-picker__header-content\">\n <button class=\"image-picker__left-arrow\">\n <svg\n class=\"image-picker__svg-left\"\n width=\"24\"\n height=\"24\"\n viewBox=\"0 0 24 24\"\n >\n <path\n d=\"M16.67 0l2.83 2.829-9.339 9.175 9.339 9.167-2.83 2.829-12.17-11.996z\"\n />\n </svg>\n </button>\n <a\n href=\"/content/images/products/antibodies/13-2004-western-blot-data.jpeg\"\n target=\"_new\"\n class=\"image-picker__main-image-link\"\n ><img\n loading=\"lazy\"\n alt=\"13-2004-western-blot-data\"\n src=\"/content/images/products/antibodies/13-2004-western-blot-data.jpeg\"\n class=\"image-picker__main-image\"\n />\n <span class=\"image-picker__main-image-caption\"\n >(Click to enlarge)</span\n ></a\n >\n <button class=\"image-picker__right-arrow\">\n <svg\n class=\"image-picker__svg-right\"\n width=\"24\"\n height=\"24\"\n viewBox=\"0 0 24 24\"\n >\n <path\n d=\"M7.33 24l-2.83-2.829 9.339-9.175-9.339-9.167 2.83-2.829 12.17 11.996z\"\n />\n </svg>\n </button>\n </div>\n <p>\n <span class=\"image-picker__span-content\"\n ><strong>Figure 4: Western blot data</strong><br />\n Western analysis of MLL1 in whole cell extracts from\n HEK293T, MCF-7, A-549, and SW620 cells. Fifty\n micrograms of lysate was resolved via SDS-PAGE and\n detected with a 1:1,000 dilution of MLL1 antibody.\n </span>\n </p>\n </div>\n <div class=\"image-picker__main-content\">\n <div class=\"image-picker__header-content\">\n <button class=\"image-picker__left-arrow\">\n <svg\n class=\"image-picker__svg-left\"\n width=\"24\"\n height=\"24\"\n viewBox=\"0 0 24 24\"\n >\n <path\n d=\"M16.67 0l2.83 2.829-9.339 9.175 9.339 9.167-2.83 2.829-12.17-11.996z\"\n />\n </svg>\n </button>\n <a\n href=\"https://cdn11.bigcommerce.com/s-y9o92/images/stencil/original/image-manager/2022.07.06-c-r-workflow-simple-rgb-ew.png?t=1697220982&_gl=1*big2yo*_ga*NDc4MDE2MzAxLjE2OTY2MjA3NTU.*_ga_WS2VZYPC6G*MTY5NzIyMDk5MC4xNS4wLjE2OTcyMjA5OTEuNTkuMC4w\"\n target=\"_new\"\n class=\"image-picker__main-image-link\"\n ><img\n loading=\"lazy\"\n alt=\"13-2004-western-blot-data\"\n src=\"https://cdn11.bigcommerce.com/s-y9o92/images/stencil/original/image-manager/2022.07.06-c-r-workflow-simple-rgb-ew.png?t=1697220982&_gl=1*big2yo*_ga*NDc4MDE2MzAxLjE2OTY2MjA3NTU.*_ga_WS2VZYPC6G*MTY5NzIyMDk5MC4xNS4wLjE2OTcyMjA5OTEuNTkuMC4w\"\n class=\"image-picker__main-image\"\n />\n <span class=\"image-picker__main-image-caption\"\n >(Click to enlarge)</span\n ></a\n >\n <button class=\"image-picker__right-arrow\">\n <svg\n class=\"image-picker__svg-right\"\n width=\"24\"\n height=\"24\"\n viewBox=\"0 0 24 24\"\n >\n <path\n d=\"M7.33 24l-2.83-2.829 9.339-9.175-9.339-9.167 2.83-2.829 12.17 11.996z\"\n />\n </svg>\n </button>\n </div>\n <p>\n <span class=\"image-picker__span-content\"\n ><strong>Figure 5: CUT&RUN Methods</strong><br />\nCUT&RUN was performed on 500k K562 cells with 0.5 µg of\n either MLL1, IgG negative control (EpiCypher\n <a\n href=\"/products/nucleosomes/snap-cutana-spike-in-controls/cutana-rabbit-igg-cut-run-negative-control-antibody\"\n >13-0042</a\n >), or H3K4me3 positive control (EpiCypher\n <a\n href=\"/products/antibodies/snap-chip-certified-antibodies/histone-h3k4me3-antibody-snap-chip-certified-cutana-cut-run-compatible\"\n >13-0041</a\n >) antibodies using the CUTANA™ ChIC/CUT&RUN Kit v2.0\n (EpiCypher\n <a\n href=\"/products/epigenetics-reagents-and-assays/cutana-chic-cut-and-run-kit\"\n >14-1048</a\n >). Library preparation was performed with 5 ng DNA (or\n the total amount recovered if less than 5 ng) using the\n CUTANA™ CUT&RUN Library Prep Kit (EpiCypher\n <a\n href=\"/products/epigenetics-reagents-and-assays/cutana-cut-and-run-library-prep-kit\"\n >14-1001/14-1002</a\n >). Both kit protocols were adapted for high throughput\n Tecan liquid handling. Libraries were run on an Illumina\n NextSeq2000 with paired-end sequencing (2x50 bp). Sample\n sequencing depth was 10.0 million reads (IgG), 13.5\n million reads (MLL1), and 8.8 million reads (H3K4me3).\n Data were aligned to the hg19 genome using Bowtie2. Data\n were filtered to remove duplicates, multi-aligned reads,\n and ENCODE DAC Exclusion List regions.\n </span>\n \n </p>\n </div>\n </div>\n <aside class=\"image-picker__right\">\n <div class=\"image-picker__gallery\">\n <img\n loading=\"lazy\"\n alt=\"13-2004-mll1-peaks\"\n src=\"/content/images/products/antibodies/13-2004-mll1-peaks.jpeg\"\n width=\"200\"\n class=\"image-picker__side-image image-picker__side-image_active\"\n role=\"button\"\n />\n <img\n loading=\"lazy\"\n alt=\"13-2004-representative-browser-tracks\"\n src=\"/content/images/products/antibodies/13-2004-representative-browser-tracks.jpeg\"\n class=\"image-picker__side-image\"\n role=\"button\"\n />\n <img\n loading=\"lazy\"\n alt=\"13-2004-immunoprecipitation\"\n src=\"/content/images/products/antibodies/13-2004-immunoprecipitation.jpeg\"\n class=\"image-picker__side-image\"\n role=\"button\"\n />\n <img\n loading=\"lazy\"\n alt=\"13-2004-western-blot-data\"\n src=\"/content/images/products/antibodies/13-2004-western-blot-data.jpeg\"\n class=\"image-picker__side-image\"\n role=\"button\"\n />\n <img\n loading=\"lazy\"\n alt=\"13-2004-western-blot-data\"\n src=\"https://cdn11.bigcommerce.com/s-y9o92/images/stencil/original/image-manager/2022.07.06-c-r-workflow-simple-rgb-ew.png?t=1697220982&_gl=1*big2yo*_ga*NDc4MDE2MzAxLjE2OTY2MjA3NTU.*_ga_WS2VZYPC6G*MTY5NzIyMDk5MC4xNS4wLjE2OTcyMjA5OTEuNTkuMC4w\"\n class=\"image-picker__side-image\"\n role=\"button\"\n />\n </div>\n </aside>\n </section>\n </div>\n </div>\n </div>\n <div id=\"prodAccordion\">\n <div id=\"ProductDescription\" class=\"Block Panel\">\n <h3 class=\"sub-title1\">Technical Information</h3>\n <div\n class=\"ProductDescriptionContainer product-droppdown__section-description\"\n >\n <div class=\"product-tech-info\">\n <div class=\"product-tech-info__line-item\">\n <div class=\"product-tech-info__line-item-left\">\n <b>Immunogen</b>\n </div>\n <div class=\"product-tech-info__line-item-right\">\n Between amino acids 720 and 780\n </div>\n </div>\n <div class=\"product-tech-info__line-item\">\n <div class=\"product-tech-info__line-item-left\">\n <b>Storage</b>\n </div>\n <div class=\"product-tech-info__line-item-right\">\n Stable for 1 year at 4°C from date of receipt.\n </div>\n </div>\n <div class=\"product-tech-info__line-item\">\n <div class=\"product-tech-info__line-item-left\">\n <b>Formulation</b>\n </div>\n <div class=\"product-tech-info__line-item-right\">\n Purified recombinant monoclonal antibody in borate buffered saline (BBS) pH 8.2,\n 0.09% sodium azide, BSA free\n </div>\n </div>\n </div>\n </div>\n </div>\n </div>\n <div id=\"prodAccordion\">\n <div id=\"ProductDescription\" class=\"Block Panel\">\n <h3 class=\"sub-title1\">Recommended Dilution</h3>\n <div\n class=\"ProductDescriptionContainer product-droppdown__section-description\"\n >\n <div class=\"product-tech-info\">\n <div class=\"product-tech-info__line-item\">\n <div class=\"product-tech-info__line-item-left\">\n <b>CUT&RUN</b>\n </div>\n <div class=\"product-tech-info__line-item-right\">0.5 µg</div>\n </div>\n <div class=\"product-tech-info__line-item\">\n <div class=\"product-tech-info__line-item-left\">\n <b>Immunoprecipitation (IP)</b>\n </div>\n <div class=\"product-tech-info__line-item-right\">\n 10 µL/0.5 mg lysate\n </div>\n </div>\n <div class=\"product-tech-info__line-item\">\n <div class=\"product-tech-info__line-item-left\">\n <b>Western Blot (WB)</b>\n </div>\n <div class=\"product-tech-info__line-item-right\">\n 1:1,000\n </div>\n </div>\n </div>\n </div>\n </div>\n </div>\n <div id=\"prodAccordion\">\n <div id=\"ProductDescription\" class=\"Block Panel\">\n <h3 class=\"sub-title1\">Gene & Protein Information</h3>\n <div\n class=\"ProductDescriptionContainer product-droppdown__section-description\"\n >\n <div class=\"product-tech-info\">\n <div class=\"product-tech-info__line-item\">\n <div class=\"product-tech-info__line-item-left\">\n <b>UniProt ID</b>\n </div>\n <div class=\"product-tech-info__line-item-right\">Q03164</div>\n </div>\n <div class=\"product-tech-info__line-item\">\n <div class=\"product-tech-info__line-item-left\">\n <b>Gene Name</b>\n </div>\n <div class=\"product-tech-info__line-item-right\">KMT2A</div>\n </div>\n <div class=\"product-tech-info__line-item\">\n <div class=\"product-tech-info__line-item-left\">\n <b>Protein Name</b>\n </div>\n <div class=\"product-tech-info__line-item-right\">\n Histone-lysine N-methyltransferase 2A\n </div>\n </div>\n <div class=\"product-tech-info__line-item\">\n <div class=\"product-tech-info__line-item-left\">\n <b>Target Size</b>\n </div>\n <div class=\"product-tech-info__line-item-right\">\n 431 kDa\n </div>\n </div>\n <div class=\"product-tech-info__line-item\">\n <div class=\"product-tech-info__line-item-left\">\n <b>Alternate Names</b>\n </div>\n <div class=\"product-tech-info__line-item-right\">\n ALL-1, CXXC7, CXXC-type zinc finger protein 7, HRX, HTRX,\n MLL, MLL1, TRX1, WDSTS\n </div>\n </div>\n </div>\n </div>\n </div>\n </div>\n <div id=\"prodAccordion\">\n <div id=\"ProductDescription\" class=\"Block Panel\">\n <h3 class=\"sub-title1\">Documents & Resources</h3>\n <div\n class=\"ProductDescriptionContainer product-droppdown__section-description\"\n >\n <div class=\"product-documents\">\n <a\n href=\"/content/documents/tds/13-2004.pdf\"\n target=\"_new\"\n class=\"product-documents__link\"\n >\n <svg\n version=\"1.1\"\n id=\"Layer_1\"\n xmlns=\"http://www.w3.org/2000/svg\"\n xmlns:xlink=\"http://www.w3.org/1999/xlink\"\n x=\"0px\"\n y=\"0px\"\n viewBox=\"0 0 228 240\"\n style=\"enable-background: new 0 0 228 240\"\n xml:space=\"preserve\"\n class=\"product-documents__icon\"\n >\n <g>\n <path\n class=\"product-documents__svg-pdf\"\n d=\"M191.92,68.77l-47.69-47.69c-1.33-1.33-3.12-2.08-5.01-2.08H45.09C41.17,19,38,22.17,38,26.09v184.36\n 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M88.07,165.63c10.35,0,15.52-5.22,15.52-15.66c0-10.4-5.17-15.59-15.52-15.59h-7.38v31.26H88.07z\"\n />\n <path\n class=\"product-documents__svg-pdf\"\n d=\"M122.57,125.68h32.84v8.49h-22.22v11.18h20.84v8.49h-20.84v20.49h-10.63V125.68z\"\n />\n </g>\n </svg>\n <span class=\"product-documents__info\"\n >Technical Datasheet</span\n >\n </a>\n </div>\n </div>\n </div>\n </div>\n <div id=\"prodAccordion\">\n <div id=\"ProductDescription\" class=\"Block Panel current\">\n <h3\n id=\"bioz\"\n style=\"padding-bottom: 5px; margin-top: 1rem\"\n class=\"sub-title1\"\n >\n Product References\n </h3>\n\n <div\n class=\"ProductDescriptionContainer product-droppdown__section-description-specific\"\n >\n <object\n id=\"wobj-3835-13-2004-q\"\n type=\"text/html\"\n data=\"https://www.bioz.com/v_widget_6_0/3835/13-2004/\"\n style=\"width: 100%; height: 193px\"\n ></object>\n <div id=\"bioz-w-pb-3835-13-2004-q-div\" style=\"width: 100%\">\n <a\n id=\"bioz-w-pb-3835-13-2004-q\"\n style=\"\n font-size: 12px;\n text-decoration: none;\n color: #4698cb;\n \"\n href=\"https://www.bioz.com/\"\n target=\"_blank\"\n ><img\n src=\"https://cdn.bioz.com/assets/favicon.png\"\n style=\"\n width: 11px;\n height: 11px;\n vertical-align: baseline;\n padding-bottom: 0px;\n margin-left: 0px;\n margin-bottom: 0px;\n float: none;\n \"\n />\n Powered by Bioz</a\n >\n <a\n style=\"\n font-size: 12px;\n text-decoration: none;\n float: right;\n color: transparent;\n \"\n href=\"https://www.bioz.com/result/13-2004/product/EpiCypher/?cn=13-2004\"\n target=\"_blank\"\n >\n See more details on Bioz</a\n >\n </div>\n </div>\n </div>\n </div>\n <div id=\"prodAccordion\">\n <div id=\"ProductDescription\" class=\"Block Panel\">\n <h3 class=\"sub-title1\">Additional Info</h3>\n <div\n class=\"ProductDescriptionContainer product-droppdown__section-description\"\n >\n <p>\n Clone BL-175-7E8 is provided for commercial sale under license\n from Bethyl Laboratories with additional lot-specific\n validation in CUT&RUN performed by EpiCypher.\n </p>\n </div>\n </div>\n </div>\n </div>\n</div>\n<script>\n $(document).ready(function () {\n var widget_micro_obj = new v_widget_obj(\"s\", [1]);\n widget_micro_obj.request_catalog_number_widget_data_internal(\n \"13-2004\",\n \"13-2004\"\n );\n });\n</script>\n<style>\n .bioz-w-header td {\n padding: 0;\n }\n td table {\n margin: 0;\n padding: 6px !important;\n }\n\n @media only screen and (max-width: 1000px) {\n .product-general-info {\n gap: 3rem;\n }\n }\n\n @media only screen and (max-width: 755px) {\n .product-general-info {\n gap: 0;\n flex-direction: column;\n }\n }\n</style>","tags":[],"warranty":"","price":{"without_tax":{"formatted":"$495.00","value":495,"currency":"USD"},"tax_label":"Sales Tax"},"detail_messages":"","availability":"","page_title":"MLL1/KMT2A Antibody | CUTANA™ CUT&RUN 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","image":{"data":"https://cdn11.bigcommerce.com/s-y9o92/images/stencil/{:size}/products/889/997/Epicypher_CUTANA_CUTRUN_LibraryPrep_Box__64431.1646406521.png?c=2","alt":"CUTANA™ CUT&RUN Library Prep Kit"},"images":[{"data":"https://cdn11.bigcommerce.com/s-y9o92/images/stencil/{:size}/products/889/997/Epicypher_CUTANA_CUTRUN_LibraryPrep_Box__64431.1646406521.png?c=2","alt":"CUTANA™ CUT&RUN Library Prep Kit"}],"date_added":"31st Jan 2022","pre_order":false,"show_cart_action":true,"has_options":true,"stock_level":null,"low_stock_level":null,"qty_in_cart":0,"custom_fields":[{"id":983,"name":"Pack Size","value":"48 Reactions"}],"num_reviews":null,"weight":{"formatted":"0.01 LBS","value":0.01},"demo":false,"price":{"without_tax":{"currency":"USD","formatted":"$1,525.00","value":1525},"tax_label":"Sales Tax"},"add_to_wishlist_url":"/wishlist.php?action=add&product_id=889"},{"id":694,"sku":null,"name":"CUTANA™ pAG-MNase for ChIC/CUT&RUN 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- Type: Monoclonal
- Host: Rabbit
- Applications: CUT&RUN, IP, WB
- Reactivity: Human
- Format: Purified
- Target Size: 431 kDa
Description
This antibody meets EpiCypher’s “CUTANA Compatible” criteria for performance in Cleavage Under Targets and Release Using Nuclease (CUT&RUN) and/or Cleavage Under Targets and Tagmentation (CUT&Tag) approaches to genomic mapping. Every lot of a CUTANA Compatible antibody is tested in the indicated CUTANA approach using EpiCypher optimized protocols and determined to yield peaks that show a genomic distribution pattern consistent with reported function(s) of the target protein. MLL1 antibody produces CUT&RUN peaks above background (Figure 1) that overlap with H3K4me3 (Figures 1-2), consistent with its known role as the catalytic subunit of the MLL1/MLL histone methyltransferase complex.
Validation Data
Figure 1: MLL1 peaks in CUT&RUN
CUT&RUN was performed as described in Figure 5. Peaks
were called with MACS2. Heatmaps show MLL1 peaks
relative to IgG negative control or H3K4me3 positive
control antibodies in aligned rows ranked by intensity
(top to bottom) and colored such that red indicates
high localized enrichment and blue denotes background
signal. MLL1 and H3K4me3 antibodies showed expected
enrichment around the TSS. Despite some observable
background in the IgG control, differential enrichment
with the target antibodies is as expected.
Figure 2: MLL1 CUT&RUN representative browser
tracks
CUT&RUN was performed as described in Figure 5. Gene
browser shots were generated using the Integrative
Genomics Viewer (IGV, Broad Institute). Two
representative loci show overlap of MLL1 and H3K4me3
peaks.
Figure 3: Immunoprecipitation data
EpiCypher MLL1 antibody (6 µL/mg lysate) was used to
immunoprecipitate whole cell lysates (1 mg per IP
reaction; 20% of IP loaded) isolated from HEK293T
cells using NETN lysis buffer. A negative control IgG
antibody and positive control antibodies targeting
MLL1 (Bethyl Laboratories) were also used to
demonstrate specificity of the IP. EpiCypher 13-2004,
Bethyl A700-010-1, and Bethyl A300-086-6 target the
same epitope, while Bethyl A300-087-3 targets a
different epitope (between amino acids 1320 and 1380)
For blotting immunoprecipitates, EpiCypher MLL1
antibody was used at a dilution of 1:1,000.
Figure 4: Western blot data
Western analysis of MLL1 in whole cell extracts from
HEK293T, MCF-7, A-549, and SW620 cells. Fifty
micrograms of lysate was resolved via SDS-PAGE and
detected with a 1:1,000 dilution of MLL1 antibody.
Figure 5: CUT&RUN Methods
CUT&RUN was performed on 500k K562 cells with 0.5 µg of
either MLL1, IgG negative control (EpiCypher
13-0042), or H3K4me3 positive control (EpiCypher
13-0041) antibodies using the CUTANA™ ChIC/CUT&RUN Kit v2.0
(EpiCypher
14-1048). Library preparation was performed with 5 ng DNA (or
the total amount recovered if less than 5 ng) using the
CUTANA™ CUT&RUN Library Prep Kit (EpiCypher
14-1001/14-1002). Both kit protocols were adapted for high throughput
Tecan liquid handling. Libraries were run on an Illumina
NextSeq2000 with paired-end sequencing (2x50 bp). Sample
sequencing depth was 10.0 million reads (IgG), 13.5
million reads (MLL1), and 8.8 million reads (H3K4me3).
Data were aligned to the hg19 genome using Bowtie2. Data
were filtered to remove duplicates, multi-aligned reads,
and ENCODE DAC Exclusion List regions.
Technical Information
Recommended Dilution
Gene & Protein Information
Documents & Resources
Product References
Additional Info
Clone BL-175-7E8 is provided for commercial sale under license from Bethyl Laboratories with additional lot-specific validation in CUT&RUN performed by EpiCypher.