MLL1/KMT2A CUTANA™ CUT&RUN Antibody

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Figure 2: MLL1 CUT&RUN peaks and functional overlap
Two representative gene loci from the CUT&RUN data in Figure 1 are shown. MLL1 peaks overlap with H3K4me3 CUT&RUN peaks (EpiCypher 13-0041), consistent with the reported function of MLL1 as an H3K4 histone methyltransferase. Images were generated using the Integrative Genomics Viewer (Broad Institute).

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Figure 3: Western blot detection of human MLL1
Whole cell lysates were isolated from HEK293T, HeLa, MCF-7, HEP-G2, A-549, and SW620 cells using NETN lysis buffer. Fifty micrograms (50 µg) of lysate were loaded onto SDS-PAGE gel and analyzed under standard western blot conditions using MLL1 antibody (1:1,000 dilution).

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Figure 4: Immunoprecipitation of human MLL1
EpiCypher MLL1 antibody (10 µL) was used to immunoprecipitate whole cell lysates isolated from HEK293T cells using NETN lysis buffer (0.5 mg per IP). A negative control IgG antibody and positive control antibodies to various MLL1 epitopes (Bethyl Laboratories) were also used to demonstrate specificity of the IP. Immunoprecipitates were loaded onto SDS-PAGE gel (20% of IP loaded) and probed via western blot with EpiCypher MLL1 antibody (1:1,000 dilution).