Protocols
Manuals, Protocols, & Guides
Choose your assay:
DNA Methylation Sequencing
CUTANA™ meCUT&RUN Kit Manual & Other Resources – use with meCUT&RUN Kit (14-1060-24)
CUTANA™ Multiomic CUT&RUN Manual & Other Resources – use with Multiomic CUT&RUN Controls Set (14-1802)
CUT&RUN
CUTANA™ ChIC / CUT&RUN Kit Manual & Other Resources – use with CUT&RUN Kit (14-1048)
CUTANA™ CUT&RUN Library Prep Kit Manual & Other Resources – use with Library Prep Kit (14-1001 and 14-1002)
SNAP-CUTANA™ Spike-in User Guide – use with SNAP-CUTANA Panels (19-1002, 19-5001, and 19-5002)
CUTANA™ CUT&RUN DIY Protocols – use with pAG-Mnase (15-1016, 15-1116) and related CUT&RUN products
CUTANA™ Quick Cleanup DNA Purification Kit Manual & Other Resources – NOTE: bead-based workflow. Use with kit 14-0052
CUTANA™ DNA Purification Kit Manual & Other Resources – NOTE: column-based workflow. Use with kit 14-0050
CUT&Tag
CUTANA™ CUT&Tag Kit Manual & Other Resources – use with CUT&Tag Kit (14-1102 and 14-1103)
SNAP-CUTANA™ Spike-in User Guide – use with SNAP-CUTANA Panels (19-1002)
CUTANA™ CUT&Tag DIY Protocols – use with pAG-Tn5 (15-1017, 15-1117) and related CUT&Tag products
CUTANA™ Quick Cleanup DNA Purification Kit Manual & Other Resources – NOTE: bead-based workflow. Use with kit 14-0052
ChIP
ChIP Protocol with Spike-in Controls – use with SNAP-ChIP Panels
SNAP-ChIP® K-MetStat Panel Manual – use with 19-1001 and 19-1100
SNAP-ChIP® K-AcylStat Panel Manual – use with 19-3001 and 19-3100
SNAP-ChIP® OncoStat Panel Manual – use with 19-2001 and 19-2100
Chromatin Remodeling Assays
EpiDyne® User Guide for Chromatin Remodeling Assays – use with EpiDyne Remodeling Enzymes and Substrates
Chromatin Binding & Activity Assays
Chromatin Methods and Protocols: The dCypher™ Approach – use with Recombinant Nucleosome substrates, Histone Binding Domains, and dCypher Services
AF9 YEATS Domain Acyl-Modified Histone Binding Assay: TR-FRET Tech Note – use with 15-0071; can be adapted for other YEATS domain proteins
AF9 YEATS Domain Acyl-Modified Histone Binding Assay: AlphaScreen Tech Note – use with 15-0071; can be adapted for other YEATS domain proteins
ENL YEATS Domain Acyl-Modified Histone Binding Assay: TR-FRET Tech Note– use with 15-0069, 15-0070; can be adapted for other YEATS domain proteins
ENL YEATS Domain Acyl-Modified Histone Binding Assay: AlphaScreen Tech Note – use with 15-0069, 15-0070; can be adapted for other YEATS domain proteins
NSD2 Histone Methyltransferase Tech Note – Can be adapted for various Nucleosome Substrates and Histone Modifying Enzymes
NSD3 Histone Methyltransferase Tech Note – Can be adapted for various Nucleosome Substrates and Histone Modifying Enzymes
SET8 Histone Methyltransferase Tech Note – Can be adapted for various Nucleosome Substrates and Histone Modifying Enzymes
Other
Desthiobiotin Nucleosome Capture & Elution Protocol – use with 16-0024