

Estrogen Receptor Alpha (N-Terminal) CUTANA™ CUT&RUN Antibody
{"AddThisServiceButtonMeta":"","add_this":[{"annotation":"","service":"facebook"},{"annotation":"","service":"email"},{"annotation":"","service":"print"},{"annotation":"","service":"twitter"},{"annotation":"","service":"linkedin"}],"add_to_wishlist_url":"/wishlist.php?action=add&product_id=840","availability":"","bulk_discount_rates":[],"can_purchase":true,"cart_url":"https://www.epicypher.com/cart.php","category":["Antibodies/CUTANA™ CUT&RUN Antibodies","Antibodies/CUTANA™ CUT&RUN Antibodies/CUT&RUN Antibodies - Chromatin-Associated Proteins","Epigenetics Kits and Reagents/CUTANA™ ChIC / CUT&RUN Assays"],"custom_fields":[{"id":"711","name":"Pack size","value":"100 µL"}],"customizations":[],"description":"<div class=\"product-general-info\">\n <ul class=\"product-general-info__list-left\">\n <li class=\"product-general-info__list-item\">\n <strong>Type: </strong>Polyclonal\n </li>\n <li class=\"product-general-info__list-item\">\n <strong>Host: </strong>Rabbit\n </li>\n <li class=\"product-general-info__list-item\">\n <strong>Target Size: </strong>66 kDa\n </li>\n </ul>\n <ul class=\"product-general-info__list-right\">\n <li class=\"product-general-info__list-item\">\n <strong>Format: </strong>Aff. Pur. IgG\n </li>\n <li class=\"product-general-info__list-item\">\n <strong>Reactivity: </strong>Human (Predicted: Mouse, Rat)\n </li>\n <li class=\"product-general-info__list-item\">\n <strong>Applications: </strong>CUT&RUN, IP, IHC\n </li>\n </ul>\n</div>\n<div class=\"service_accordion product-droppdown\">\n <div class=\"container\">\n <div id=\"prodAccordion\">\n <div id=\"ProductDescription\" class=\"Block Panel current\">\n <h3 class=\"sub-title1\">Description</h3>\n <div\n class=\"ProductDescriptionContainer product-droppdown__section-description-specific\"\n >\n <p>\n This antibody meets EpiCypher’s “CUTANA Compatible” criteria for\n performance in Cleavage Under Targets and Release Using Nuclease\n (CUT&RUN) and/or Cleavage Under Targets and Tagmentation\n (CUT&Tag) approaches to genomic mapping. Every lot of a CUTANA\n Compatible antibody is tested in the indicated CUTANA approach using\n <a href=\"https://www.epicypher.com/resources/protocols\"\n >EpiCypher optimized protocols </a\n >and determined to yield peaks that show a genomic distribution\n pattern consistent with reported function(s) of the target protein.\n Estrogen Receptor Alpha N-terminal (ER alpha N-term) antibody shows\n CUT&RUN peaks in response to estradiol stimulation (<strong\n >Figure 1</strong\n >) that overlap with known estrogen response element (ERE) binding\n motifs (<strong>Figure 2</strong>). Overlap is further observed with\n peaks from an antibody to a different ER alpha epitope (C-term;\n <a\n href=\"https://www.epicypher.com/products/antibodies/cutana-cut-run-compatible-antibodies/estrogen-receptor-alpha-c-terminal-cutana-cut-run-antibody\"\n >13-2012</a\n >) and NCOA3/SRC3 (<a\n href=\"/products/antibodies/cutana-cut-run-compatible-antibodies/ncoa3-src3-cutana-cut-run-antibody\"\n >13-2013</a\n >), which coactivates ER-mediated transcription (1)\n <strong>(Figure 2)</strong>.\n </p>\n </div>\n </div>\n </div>\n <div id=\"prodAccordion\">\n <div id=\"ProductDescription\" class=\"Block Panel current\">\n <h3 class=\"sub-title1\">Validation Data</h3>\n <div\n class=\"ProductDescriptionContainer product-droppdown__section-description-specific\"\n >\n <section class=\"image-picker\">\n <div class=\"image-picker__left\">\n <div\n class=\"image-picker__main-content_active image-picker__main-content\"\n >\n <div class=\"image-picker__header-content\">\n <button class=\"image-picker__left-arrow\">\n <svg\n class=\"image-picker__svg-left\"\n width=\"24\"\n height=\"24\"\n viewBox=\"0 0 24 24\"\n >\n <path\n d=\"M16.67 0l2.83 2.829-9.339 9.175 9.339 9.167-2.83 2.829-12.17-11.996z\"\n />\n </svg>\n </button>\n <a\n href=\"/content/images/products/antibodies/13_2011_Heatmap.jpeg\"\n target=\"_new\"\n class=\"image-picker__main-image-link\"\n ><img\n alt=\"13_2011_Heatmap\"\n src=\"/content/images/products/antibodies/13_2011_Heatmap.jpeg\"\n class=\"image-picker__main-image\"\n />\n <span class=\"image-picker__main-image-caption\"\n >(Click to enlarge)</span\n ></a\n >\n <button class=\"image-picker__right-arrow\">\n <svg\n class=\"image-picker__svg-right\"\n width=\"24\"\n height=\"24\"\n viewBox=\"0 0 24 24\"\n >\n <path\n d=\"M7.33 24l-2.83-2.829 9.339-9.175-9.339-9.167 2.83-2.829 12.17 11.996z\"\n />\n </svg>\n </button>\n </div>\n <p>\n <span class=\"image-picker__span-content\"\n ><strong\n >Figure 1: ER alpha N-term enrichment in CUT&RUN </strong\n ><br />\n Serum-starved MCF7 cells were treated with 100 nM estradiol\n (E2) or vehicle control for 45 minutes. CUT&RUN was\n performed using 500,000 cells with 0.5 µg of each indicated\n antibody (gray text). Heatmap shows CUT&RUN enrichment in\n aligned rows ranked by intensity (top to bottom; relative to\n ER alpha C-term, <a\n href=\"https://www.epicypher.com/products/antibodies/cutana-cut-run-compatible-antibodies/estrogen-receptor-alpha-c-terminal-cutana-cut-run-antibody\"\n >13-2012</a\n >). Red indicates high localized enrichment and blue\n denotes background signal.\n </span>\n </p>\n </div>\n <div class=\"image-picker__main-content\">\n <div class=\"image-picker__header-content\">\n <button class=\"image-picker__left-arrow\">\n <svg\n class=\"image-picker__svg-left\"\n width=\"24\"\n height=\"24\"\n viewBox=\"0 0 24 24\"\n >\n <path\n d=\"M16.67 0l2.83 2.829-9.339 9.175 9.339 9.167-2.83 2.829-12.17-11.996z\"\n />\n </svg>\n </button>\n <a\n href=\"/content/images/products/antibodies/13_2011_Peaks.jpeg\"\n target=\"_new\"\n class=\"image-picker__main-image-link\"\n ><img\n alt=\"13_2011_Peaks\"\n src=\"/content/images/products/antibodies/13_2011_Peaks.jpeg\"\n class=\"image-picker__main-image\"\n />\n <span class=\"image-picker__main-image-caption\"\n >(Click to enlarge)</span\n ></a\n >\n <button class=\"image-picker__right-arrow\">\n <svg\n class=\"image-picker__svg-right\"\n width=\"24\"\n height=\"24\"\n viewBox=\"0 0 24 24\"\n >\n <path\n d=\"M7.33 24l-2.83-2.829 9.339-9.175-9.339-9.167 2.83-2.829 12.17 11.996z\"\n />\n </svg>\n </button>\n </div>\n <p>\n <span class=\"image-picker__span-content\"\n ><strong\n >Figure 2: ER alpha N-term peak analysis in\n CUT&RUN</strong\n ><br />\n Peaks from the E2-treated samples in Figure 1 were called\n using MACS2. <strong>(A)</strong> The number of ER alpha\n N-term peaks which fall into distinct classes of\n functionally annotated genomic regions is plotted.\n <strong>(B)</strong> Homer analysis determined that the ERE\n consensus motif, represented as a sequence logo position\n weight matrix, was enriched under ER alpha N-term peaks.\n <strong>(C)</strong> The number of ER alpha N-term peaks\n containing consensus motifs from panel B is shown by Venn\n Diagram. <strong>(D)</strong> The number of ER alpha N-term\n peaks that overlap with ER alpha C-term (<a\n href=\"https://www.epicypher.com/products/antibodies/cutana-cut-run-compatible-antibodies/estrogen-receptor-alpha-c-terminal-cutana-cut-run-antibody\"\n >13-2012</a\n >) and NCOA3/SRC3 (<a\n href=\"/products/antibodies/cutana-cut-run-compatible-antibodies/ncoa3-src3-cutana-cut-run-antibody\"\n >13-2013</a\n >) antibodies are represented by Venn Diagram.\n </span>\n </p>\n </div>\n <div class=\"image-picker__main-content\">\n <div class=\"image-picker__header-content\">\n <button class=\"image-picker__left-arrow\">\n <svg\n class=\"image-picker__svg-left\"\n width=\"24\"\n height=\"24\"\n viewBox=\"0 0 24 24\"\n >\n <path\n d=\"M16.67 0l2.83 2.829-9.339 9.175 9.339 9.167-2.83 2.829-12.17-11.996z\"\n />\n </svg>\n </button>\n <a\n href=\"/content/images/products/antibodies/13_2011_IP.jpeg\"\n target=\"_new\"\n class=\"image-picker__main-image-link\"\n ><img\n alt=\"13_2011_IP\"\n src=\"/content/images/products/antibodies/13_2011_IP.jpeg\"\n class=\"image-picker__main-image\"\n />\n <span class=\"image-picker__main-image-caption\"\n >(Click to enlarge)</span\n ></a\n >\n <button class=\"image-picker__right-arrow\">\n <svg\n class=\"image-picker__svg-right\"\n width=\"24\"\n height=\"24\"\n viewBox=\"0 0 24 24\"\n >\n <path\n d=\"M7.33 24l-2.83-2.829 9.339-9.175-9.339-9.167 2.83-2.829 12.17 11.996z\"\n />\n </svg>\n </button>\n </div>\n <p>\n <span class=\"image-picker__span-content\"\n ><strong\n >Figure 3: Immunoprecipitation of human ER alpha</strong\n ><br />\n EpiCypher ER alpha N-term antibody (3 µg) was used to\n immunoprecipitate whole cell lysates isolated from MCF7\n cells (1.0 mg per IP). A negative control IgG antibody and\n positive control antibody to a different ER alpha epitopes\n (EpiCypher\n <a\n href=\"https://www.epicypher.com/products/antibodies/cutana-cut-run-compatible-antibodies/estrogen-receptor-alpha-c-terminal-cutana-cut-run-antibody\"\n >13-2012</a\n >\n and Bethyl Laboratories) were also used to demonstrate\n specificity of the IP. Immunoprecipitates were loaded onto\n 4-8% SDS-PAGE gel (25% of IP loaded) and probed via western\n blot with EpiCypher\n <a\n href=\"https://www.epicypher.com/products/antibodies/cutana-cut-run-compatible-antibodies/estrogen-receptor-alpha-c-terminal-cutana-cut-run-antibody\"\n >13-2012</a\n >\n ER alpha C-term antibody (0.1 µg/mL).\n </span>\n </p>\n </div>\n <div class=\"image-picker__main-content\">\n <div class=\"image-picker__header-content\">\n <button class=\"image-picker__left-arrow\">\n <svg\n class=\"image-picker__svg-left\"\n width=\"24\"\n height=\"24\"\n viewBox=\"0 0 24 24\"\n >\n <path\n d=\"M16.67 0l2.83 2.829-9.339 9.175 9.339 9.167-2.83 2.829-12.17-11.996z\"\n />\n </svg>\n </button>\n <a\n href=\"/content/images/products/antibodies/13_2011_IHC.jpeg\"\n target=\"_new\"\n class=\"image-picker__main-image-link\"\n ><img\n alt=\"13_2011_IHC\"\n src=\"/content/images/products/antibodies/13_2011_IHC.jpeg\"\n class=\"image-picker__main-image\"\n />\n <span class=\"image-picker__main-image-caption\"\n >(Click to enlarge)</span\n ></a\n >\n <button class=\"image-picker__right-arrow\">\n <svg\n class=\"image-picker__svg-right\"\n width=\"24\"\n height=\"24\"\n viewBox=\"0 0 24 24\"\n >\n <path\n d=\"M7.33 24l-2.83-2.829 9.339-9.175-9.339-9.167 2.83-2.829 12.17 11.996z\"\n />\n </svg>\n </button>\n </div>\n <p>\n <span class=\"image-picker__span-content\"\n ><strong\n >Figure 4: Immunohistochemistry detection of human ER\n alpha</strong\n ><br />\n FFPE section of human breast examined using ER alpha N-term\n antibody (1:2,500 dilution, 0.4 µg/mL).\n </span>\n </p>\n </div>\n </div>\n <aside class=\"image-picker__right\">\n <div class=\"image-picker__gallery\">\n <img\n alt=\"13_2011_Heatmap\"\n src=\"/content/images/products/antibodies/13_2011_Heatmap.jpeg\"\n width=\"200\"\n class=\"image-picker__side-image image-picker__side-image_active\"\n role=\"button\"\n />\n <img\n alt=\"13_2011_Peaks\"\n src=\"/content/images/products/antibodies/13_2011_Peaks.jpeg\"\n class=\"image-picker__side-image\"\n role=\"button\"\n />\n <img\n alt=\"13_2011_IP\"\n src=\"/content/images/products/antibodies/13_2011_IP.jpeg\"\n class=\"image-picker__side-image\"\n role=\"button\"\n />\n <img\n alt=\"13_2011_IHC\"\n src=\"/content/images/products/antibodies/13_2011_IHC.jpeg\"\n class=\"image-picker__side-image\"\n role=\"button\"\n />\n </div>\n </aside>\n </section>\n </div>\n </div>\n </div>\n <div id=\"prodAccordion\">\n <div id=\"ProductDescription\" class=\"Block Panel\">\n <h3 class=\"sub-title1\">Technical Information</h3>\n <div\n class=\"ProductDescriptionContainer product-droppdown__section-description\"\n >\n <div class=\"product-tech-info\">\n <div class=\"product-tech-info__line-item\">\n <div class=\"product-tech-info__line-item-left\">\n <b>Immunogen</b>\n </div>\n\n <div class=\"product-tech-info__line-item-right\">\n A synthetic peptide corresponding to human ER alpha amino acids\n 1-50.\n </div>\n </div>\n <div class=\"product-tech-info__line-item\">\n <div class=\"product-tech-info__line-item-left\">\n <b>Formulation</b>\n </div>\n <div class=\"product-tech-info__line-item-right\">\n Antigen affinity-purified antibody (1.0 mg/mL) in\n Tris-citrate/phosphate buffer pH 7 to 8, 0.09% sodium azide\n </div>\n </div>\n <div class=\"product-tech-info__line-item\">\n <div class=\"product-tech-info__line-item-left\">\n <b>Storage and Stability</b>\n </div>\n <div class=\"product-tech-info__line-item-right\">\n Stable for 1 year at 4°C from date of receipt.\n </div>\n </div>\n </div>\n </div>\n </div>\n </div>\n <div id=\"prodAccordion\">\n <div id=\"ProductDescription\" class=\"Block Panel\">\n <h3 class=\"sub-title1\">Application Notes</h3>\n <div\n class=\"ProductDescriptionContainer product-droppdown__section-description\"\n >\n <p><strong>Recommended Dilutions:</strong></p>\n <p><strong>CUT&RUN:</strong> 0.5 µg</p>\n <p><strong>IHC:</strong> 1:1000 - 1:5,000*</p>\n <p><strong>IP:</strong> 3 - 5 µg/mg lysate</p>\n <p>\n <em\n >*Epitope retrieval with Tris-EDTA pH 9.0 recommended for FFPE\n tissue</em\n >\n </p>\n </div>\n </div>\n </div>\n <div id=\"prodAccordion\">\n <div id=\"ProductDescription\" class=\"Block Panel\">\n <h3 class=\"sub-title1\">References</h3>\n <div\n class=\"ProductDescriptionContainer product-droppdown__section-description\"\n >\n <strong>Background References:</strong>\n <br />\n 1. Wagner <em>et al.</em> <em>BMC Cancer </em> (2013). PMID:\n <a\n href=\"https://pubmed.ncbi.nlm.nih.gov/24304549/\"\n title=\"NCOA3 is a selective co-activator of estrogen receptor α-mediated transactivation of PLAC1 in MCF-7 breast cancer cells\"\n target=\"new\"\n >\n 24304549</a\n ><br />\n </div>\n </div>\n </div>\n <div id=\"prodAccordion\">\n <div id=\"ProductDescription\" class=\"Block Panel\">\n <h3 class=\"sub-title1\">Documents & Resources</h3>\n <div\n class=\"ProductDescriptionContainer product-droppdown__section-description\"\n >\n <div class=\"product-documents\">\n <a\n href=\"/content/documents/tds/13-2011.pdf\"\n target=\"_new\"\n class=\"product-documents__link\"\n >\n <svg\n version=\"1.1\"\n id=\"Layer_1\"\n xmlns=\"http://www.w3.org/2000/svg\"\n xmlns:xlink=\"http://www.w3.org/1999/xlink\"\n x=\"0px\"\n y=\"0px\"\n viewBox=\"0 0 228 240\"\n style=\"enable-background: new 0 0 228 240\"\n xml:space=\"preserve\"\n class=\"product-documents__icon\"\n >\n <g>\n <path\n class=\"product-documents__svg-pdf\"\n d=\"M191.92,68.77l-47.69-47.69c-1.33-1.33-3.12-2.08-5.01-2.08H45.09C41.17,19,38,22.17,38,26.09v184.36\n c0,3.92,3.17,7.09,7.09,7.09h141.82c3.92,0,7.09-3.17,7.09-7.09V73.8C194,71.92,193.25,70.1,191.92,68.77z M177.65,77.06h-41.7\n v-41.7L177.65,77.06z M178.05,201.59H53.95V34.95h66.92v47.86c0,5.14,4.17,9.31,9.31,9.31h47.86V201.59z\"\n />\n </g>\n <rect\n x=\"20\"\n y=\"112\"\n class=\"product-documents__svg-background\"\n width=\"146\"\n height=\"76\"\n />\n <g>\n <path\n class=\"product-documents__svg-pdf\"\n d=\"M23.83,125.68h22.36c5.29,0,9.41,1.33,12.35,4c2.94,2.67,4.42,6.39,4.42,11.18c0,4.78-1.47,8.51-4.42,11.18\n c-2.94,2.67-7.06,4-12.35,4H34.59v18.29H23.83V125.68z M44.81,147.9c5.38,0,8.07-2.32,8.07-6.97c0-2.39-0.67-4.16-2-5.31\n c-1.33-1.15-3.36-1.73-6.07-1.73H34.59v14.01H44.81z\"\n />\n <path\n class=\"product-documents__svg-pdf\"\n d=\"M69.92,125.68h18.91c5.29,0,9.84,0.97,13.66,2.9c3.82,1.93,6.74,4.72,8.76,8.35\n c2.02,3.63,3.04,7.98,3.04,13.04c0,5.06-1,9.42-3,13.08c-2,3.66-4.91,6.45-8.73,8.38c-3.82,1.93-8.4,2.9-13.73,2.9H69.92V125.68z\n M88.07,165.63c10.35,0,15.52-5.22,15.52-15.66c0-10.4-5.17-15.59-15.52-15.59h-7.38v31.26H88.07z\"\n />\n <path\n class=\"product-documents__svg-pdf\"\n d=\"M122.57,125.68h32.84v8.49h-22.22v11.18h20.84v8.49h-20.84v20.49h-10.63V125.68z\"\n />\n </g>\n </svg>\n <span class=\"product-documents__info\">Technical Datasheet</span>\n </a>\n </div>\n </div>\n </div>\n </div>\n <div id=\"prodAccordion\">\n <div id=\"ProductDescription\" class=\"Block Panel\">\n <h3 class=\"sub-title1\">Additional Info</h3>\n <div\n class=\"ProductDescriptionContainer product-droppdown__section-description\"\n >\n <p>\n This product is provided for commercial sale under license from\n Bethyl Laboratories, Inc.\n </p>\n\n <strong>Applications Key:</strong> <br /><br />\n\n ChIP: Chromatin immunoprecipitation<br />\n CUT&RUN: Cleavage Under Targets and Release Using Nuclease<br />\n CUT&Tag: Cleavage Under Targets and Tagmentation<br />\n E: ELISA<br />\n FACS: Flow cytometry<br />\n ICC: Immunocytochemistry<br />\n IF: Immunofluorescence<br />\n IHC: Immunohistochemistry<br />\n IP: Immunoprecipitation<br />\n L: Luminex<br />\n WB: Western Blot<br /><br />\n\n <strong>Reactivity Key:</strong> <br /><br />\n\n B: Bovine<br />\n Ce: <em>C. elegans</em><br />\n Ch: Chicken<br />\n Dm: <em>Drosophila</em><br />\n Eu: Eukaryote<br />\n H: Human<br />\n M: Mouse<br />\n Ma: Mammal<br />\n R: Rat<br />\n Sc: <em>S. cerevisiae</em><br />\n Sp: <em>S. pombe</em><br />\n WR: Wide Range (predicted)<br />\n X: Xenopus<br />\n Z: Zebrafish<br />\n </div>\n </div>\n </div>\n </div>\n</div>\n","detail_messages":"","gift_wrapping_available":false,"gtin":null,"id":"840","images":[{"alt":"Estrogen Receptor Alpha N-Terminal CUTANA CUTandRUN Antibody","data":"https://cdn11.bigcommerce.com/s-y9o92/images/stencil/{:size}/products/840/955/estrogen-receptor-alpha-n-terminal-cutana-cutandrun-antibody__87451.1645734547.jpg?c=2"}],"main_image":{"alt":"Estrogen Receptor Alpha N-Terminal CUTANA CUTandRUN Antibody","data":"https://cdn11.bigcommerce.com/s-y9o92/images/stencil/{:size}/products/840/955/estrogen-receptor-alpha-n-terminal-cutana-cutandrun-antibody__87451.1645734547.jpg?c=2"},"max_purchase_quantity":0,"meta_description":"Polyclonal anti-Estrogen Receptor Alpha antibody validated for CUT&RUN, IP, IHC. Rigorously tested for reliable and robust performance in CUT&RUN assays.","meta_keywords":"estrogen receptor alpha antibody, estrogen receptor antibody, era antibody, erα antibody, CUT&RUN antibody","min_purchase_quantity":0,"mpn":null,"num_reviews":0,"options":[],"page_title":"Estrogen Receptor Alpha (N-Terminal) Antibody | CUTANA™ CUT&RUN Compatible","price":{"tax_label":"Sales Tax","without_tax":{"currency":"USD","formatted":"$445.00","value":445}},"rating":0,"related_products":[{"add_to_cart_url":"https://www.epicypher.com/cart.php?action=add&product_id=798","add_to_wishlist_url":"/wishlist.php?action=add&product_id=798","availability":"","brand":{"name":null},"category":["Antibodies","Antibodies/CUTANA™ CUT&RUN Antibodies","Antibodies/CUTANA™ CUT&RUN Antibodies/CUT&RUN Antibodies - Chromatin-Associated Proteins","Epigenetics Kits and Reagents/CUTANA™ ChIC / CUT&RUN Assays"],"custom_fields":[{"id":655,"name":"Pack size","value":"50 µL"}],"date_added":"25th Nov 2020","demo":false,"has_options":false,"id":798,"image":{"alt":"BRD4 CUTANA CUTandRUN Antibody","data":"https://cdn11.bigcommerce.com/s-y9o92/images/stencil/{:size}/products/798/933/brd4-cutana-cutandrun-antibody__13949.1645734524.jpg?c=2"},"images":[{"alt":"BRD4 CUTANA CUTandRUN Antibody","data":"https://cdn11.bigcommerce.com/s-y9o92/images/stencil/{:size}/products/798/933/brd4-cutana-cutandrun-antibody__13949.1645734524.jpg?c=2"}],"low_stock_level":null,"name":"BRD4 CUTANA™ CUT&RUN Antibody","num_reviews":null,"pre_order":false,"price":{"tax_label":"Sales Tax","without_tax":{"currency":"USD","formatted":"$445.00","value":445}},"qty_in_cart":0,"rating":null,"show_cart_action":true,"sku":"13-2003","stock_level":null,"summary":"\n \n \n Type: Polyclonal\n \n \n Host: Rabbit\n \n \n Target Size: 152 kD","url":"https://www.epicypher.com/products/antibodies/brd4-cutana-cut-run-antibody","weight":{"formatted":"0.01 LBS","value":0.01}},{"add_to_cart_url":"https://www.epicypher.com/cart.php?action=add&product_id=804","add_to_wishlist_url":"/wishlist.php?action=add&product_id=804","availability":"","brand":{"name":null},"category":["Antibodies","Antibodies/CUTANA™ CUT&RUN Antibodies","Antibodies/CUTANA™ CUT&RUN Antibodies/CUT&RUN Antibodies - 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- Type: Polyclonal
- Host: Rabbit
- Target Size: 66 kDa
- Format: Aff. Pur. IgG
- Reactivity: Human (Predicted: Mouse, Rat)
- Applications: CUT&RUN, IP, IHC
Description
This antibody meets EpiCypher’s “CUTANA Compatible” criteria for performance in Cleavage Under Targets and Release Using Nuclease (CUT&RUN) and/or Cleavage Under Targets and Tagmentation (CUT&Tag) approaches to genomic mapping. Every lot of a CUTANA Compatible antibody is tested in the indicated CUTANA approach using EpiCypher optimized protocols and determined to yield peaks that show a genomic distribution pattern consistent with reported function(s) of the target protein. Estrogen Receptor Alpha N-terminal (ER alpha N-term) antibody shows CUT&RUN peaks in response to estradiol stimulation (Figure 1) that overlap with known estrogen response element (ERE) binding motifs (Figure 2). Overlap is further observed with peaks from an antibody to a different ER alpha epitope (C-term; 13-2012) and NCOA3/SRC3 (13-2013), which coactivates ER-mediated transcription (1) (Figure 2).
Validation Data
Figure 1: ER alpha N-term enrichment in CUT&RUN
Serum-starved MCF7 cells were treated with 100 nM estradiol
(E2) or vehicle control for 45 minutes. CUT&RUN was
performed using 500,000 cells with 0.5 µg of each indicated
antibody (gray text). Heatmap shows CUT&RUN enrichment in
aligned rows ranked by intensity (top to bottom; relative to
ER alpha C-term, 13-2012). Red indicates high localized enrichment and blue
denotes background signal.
Figure 2: ER alpha N-term peak analysis in
CUT&RUN
Peaks from the E2-treated samples in Figure 1 were called
using MACS2. (A) The number of ER alpha
N-term peaks which fall into distinct classes of
functionally annotated genomic regions is plotted.
(B) Homer analysis determined that the ERE
consensus motif, represented as a sequence logo position
weight matrix, was enriched under ER alpha N-term peaks.
(C) The number of ER alpha N-term peaks
containing consensus motifs from panel B is shown by Venn
Diagram. (D) The number of ER alpha N-term
peaks that overlap with ER alpha C-term (13-2012) and NCOA3/SRC3 (13-2013) antibodies are represented by Venn Diagram.
Figure 3: Immunoprecipitation of human ER alpha
EpiCypher ER alpha N-term antibody (3 µg) was used to
immunoprecipitate whole cell lysates isolated from MCF7
cells (1.0 mg per IP). A negative control IgG antibody and
positive control antibody to a different ER alpha epitopes
(EpiCypher
13-2012
and Bethyl Laboratories) were also used to demonstrate
specificity of the IP. Immunoprecipitates were loaded onto
4-8% SDS-PAGE gel (25% of IP loaded) and probed via western
blot with EpiCypher
13-2012
ER alpha C-term antibody (0.1 µg/mL).
Figure 4: Immunohistochemistry detection of human ER
alpha
FFPE section of human breast examined using ER alpha N-term
antibody (1:2,500 dilution, 0.4 µg/mL).
Technical Information
Application Notes
Recommended Dilutions:
CUT&RUN: 0.5 µg
IHC: 1:1000 - 1:5,000*
IP: 3 - 5 µg/mg lysate
*Epitope retrieval with Tris-EDTA pH 9.0 recommended for FFPE tissue
References
Documents & Resources
Additional Info
This product is provided for commercial sale under license from Bethyl Laboratories, Inc.
Applications Key:ChIP: Chromatin immunoprecipitation
CUT&RUN: Cleavage Under Targets and Release Using Nuclease
CUT&Tag: Cleavage Under Targets and Tagmentation
E: ELISA
FACS: Flow cytometry
ICC: Immunocytochemistry
IF: Immunofluorescence
IHC: Immunohistochemistry
IP: Immunoprecipitation
L: Luminex
WB: Western Blot
Reactivity Key:
B: Bovine
Ce: C. elegans
Ch: Chicken
Dm: Drosophila
Eu: Eukaryote
H: Human
M: Mouse
Ma: Mammal
R: Rat
Sc: S. cerevisiae
Sp: S. pombe
WR: Wide Range (predicted)
X: Xenopus
Z: Zebrafish
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