SMARCA4 Chromatin Remodeling Enzyme (Human BRG1)

$505.00
SKU: 15-1014
Pack size: 100 reactions
Type:  Remodeler/ATPase Host: SF9 cells
Mol Wgt.: 182.7 kDa Epitope Tag:  FLAG


SMARCA4 Remodeling Enzyme Description: Full length recombinant human SMARCA4 (BRG1) Remodeling Enzyme produced in SF9 cells. SMARCA4 is a crucial component of the SWI/SNF complex. It is an ATPdependent chromatin remodeling enzyme that regulates nucleosome spacing.

This is product includes 5x SMARCA4 Remodeling Assay Buffer



SMARCA4 Remodeling Enzyme Formulation: SMARCA4 at 0.85 mg/ml in 4.3 μl of 20 mM Tris HCl pH 7.9, 150 mM NaCl, 2 mM MgCl2, 0.2 mM EDTA, 10 mM beta-glycerophosphate, 0.01% NP-40, 0.2 mM PMSF, 0.5 mM benzamidine, 1 mM DTT, 15% glycerol, 2 μg/ml leupeptin, 1 μg/ml aprotinin, 0.4 mg/ml recombinant human insulin, 0.4 mg/ml 3x FLAG peptide. Molarity: 4.7 μM

Storage and Stability: Stable for six months at -80°C from date of receipt. For best results, aliquot and avoid multiple freeze/thaws.

Application Notes: This product is sufficient to perform 100 remodeling reactions using EpiDyne®-FRET substrate (Catalog No. 16 -4201). A single reaction is defined as 10 μl containing [20 nM SMARCA4/ 20 nM EpiDyne®-FRET/ 2.5 mM rATP] and remodels to completion in <20 minutes. 5x SMARCA Remodeling Assay Buffer is included (100 mM Tris HCl, pH 7.5, 250 mM KCl, 15 mM MgCl2, 0.05% (w/v) BSA, 0.05% (v/v) Tween 20). For best results, make single-use aliquots when first thawed.

References:


View technical datasheet for this product. 15-1014 Datasheet
15-1014 ATP-dependent Chromatin Remodeling

ATP-dependent Chromatin Remodeling Assay: EpiDyne®-FRET Chromatin Remodeling Substrate (20 nM; Catalog No. 16-4201) incubated with SMARCA4 Remodeling Enzyme Complex (concentrations indicated). Curves denote FRET efficiency/chromatin remodeling.
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15-1014 Protein Gel Data

Protein Gel Data: SMARCA4 Remodeling Enzyme (1μg) was run on an SDS-PAGE gel and stained with Coomassie blue. The migration and moleuclar weight of the protein standards are indicated.
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