SKU: 21-0013
{"AddThisServiceButtonMeta":"","add_this":[{"annotation":"","service":"facebook"},{"annotation":"","service":"email"},{"annotation":"","service":"print"},{"annotation":"","service":"twitter"},{"annotation":"","service":"linkedin"}],"add_to_wishlist_url":"/wishlist.php?action=add&product_id=623","availability":"","bulk_discount_rates":[],"can_purchase":true,"cart_url":"https://www.epicypher.com/cart.php","category":["Recombinant Proteins/Modifying Enzymes"],"custom_fields":[{"id":"587","name":"Pack Size","value":"500 µL"}],"customizations":[],"description":"<table width=\"100%\" border=\"0\" cellspacing=\"2\" cellpadding=\"2\">\n \n <tbody>\n \n <tr valign=\"top\">\n \n <td>\n \n \n \n <table width=\"95%\" border=\"0\" cellspacing=\"1\" cellpadding=\"1\">\n \n <tbody>\n \n <tr>\n \n <td>\n \n <table width=\"100%\" border=\"0\" cellspacing=\"0\" cellpadding=\"0\">\n \n <tbody>\n \n <tr></tr>\n \n </tbody>\n \n </table>\n \n </td>\n \n </tr>\n \n <tr>\n \n <td valign=\"top\"><span style=\"font-family: Arial; \" class=\"Apple-style-span\"><span style=\"font-size: 12pt; \" class=\"Apple-style-span\"><b><span class=\"Apple-style-span\" style=\"font-family: Arial; \"><span class=\"Apple-style-span\" style=\"font-size: 12pt; \"><b>5x ACF Remodeling Assay Buffer<br>\n </b></span></span> Description:</b> 5x concentrated reaction buffer for use with ACF Remodeling Enzyme Complex (Catalog No. 15-1013). Optimized for use with recombinant Drosophila ACF Remodeling Enzyme Complex, assembled from Acf1-FLAG and ISWI produced in SF9 cells. ACF is an ATP-dependent chromatin remodeling complex that regulates nucleosome spacing.</span></span>\n \n </td>\n \n </tr>\n \n <tr>\n \n <td valign=\"top\"><span style=\"font-family: Arial; \" class=\"Apple-style-span\"><span style=\"font-size: 12pt; \" class=\"Apple-style-span\"><b><span class=\"Apple-style-span\" style=\"font-family: Arial; \"><span class=\"Apple-style-span\" style=\"font-size: 12pt; \"><b>ACF Remodeling Enzyme Complex</b></span></span> Formulation:</b> 5x ACF Remodeling Assay Buffer (100 mM Tris HCl, pH 7.5, 250 mM KCl, 15 mM MgCl2, 0.05% (w/v) BSA, 0.05% (v/v) Tween 20) is formulated for use with ACF Remodeling Enzyme Complex.<br />\n \n <br />\n \n </span></span>\n \n </td>\n \n </tr>\n \n <tr>\n \n <td valign=\"top\"><span style=\"font-family: Arial; \" class=\"Apple-style-span\"><span style=\"font-size: 12pt; \" class=\"Apple-style-span\"><b>Storage and Stability:</b> Stable for six months at 4°C from date of receipt.<br />\n \n <br />\n \n </span></span>\n \n </td>\n \n </tr>\n \n <tr>\n \n <td valign=\"top\"><p><span style=\"font-family: Arial; \" class=\"Apple-style-span\"><span style=\"font-size: 12pt; \" class=\"Apple-style-span\"><b>Application Notes:</b> This produt is formulated to perform remodeling reactions using ACF Remodeling Enzyme Complex and EpiDyne®-FRET substrate in a reaction defined as 10 μl containing [5 nM ACF Complex/ 20 nM EpiDyne®-FRET/ 50 μM rATP] and remodels to completion in <20 minutes. For best results, store 5x ACF Remodeling Assay buffer at 4ºC and avoid freezing.</span></span></p>\n <p><span style=\"font-family: Arial; \" class=\"Apple-style-span\"><span style=\"font-size: 12pt; \" class=\"Apple-style-span\"> For further information, please contact techsupport@epicypher.com or refer to the EpiDyne®- FRET Technote (TN#106; available at www.epicypher.com/technotes).</span></span><span style=\"font-family: Arial; \" class=\"Apple-style-span\"><span style=\"font-size: 12pt; \" class=\"Apple-style-span\"><br />\n \n </span></span></p>\n \n </td>\n \n </tr>\n \n <tr>\n \n <td valign=\"top\"><p><span style=\"font-family: Arial; \" class=\"Apple-style-span\"><span style=\"font-size: 12pt; \" class=\"Apple-style-span\"><br />\n \n </span></span><br />\n \n </p>\n \n </td>\n \n </tr>\n \n <tr>\n \n <td valign=\"top\"><span style=\"font-family: Arial; \" class=\"Apple-style-span\"><span style=\"font-size: 12pt; \" class=\"Apple-style-span\">View technical datasheet for this product. <a href=\"https://www.epicypher.com/content/documents/tds/21-0013.pdf\" target=\"_blank\"><img src=\"https://cdn7.bigcommerce.com/s-y9o92/content/documents/tds/icon.png\" alt=\"21-0013 Datasheet\" width=\"30\" height=\"40\" /></a></span></span>\n \n </td>\n \n </tr>\n \n </tbody>\n \n </table>\n \n </td>\n \n <td>\n \n <table width=\"100%\" border=\"0\" cellspacing=\"3\" cellpadding=\"3\">\n \n <tbody>\n \n <tr>\n \n <td align=\"center\"><a href=\"https://www.epicypher.com/content/images/products/proteins/15_1013_ATP_dep_chromatin_remodeling.jpg\" target=\"_new\">\n \n <img src=\"https://cdn7.bigcommerce.com/s-y9o92/content/images/products/proteins/15_1013_ATP_dep_chromatin_remodeling.jpg\" alt=\"15-1013 ATP-dependent Chromatin Remodeling \" width=\"250\" height=\"183\" /></a>\n \n </td>\n \n </tr>\n \n <tr>\n \n <td align=\"left\" valign=\"top\">\n \n <p><span style=\"font-family: Arial; \" class=\"Apple-style-span\"><span style=\"font-size: 10pt; \" class=\"Apple-style-span\"><strong>ATP-dependent Chromatin Remodeling Assay:</strong> EpiDyne®- FRET Chromatin Remodeling Substrate (20 nM; Catalog No. 16-4201) incubated with ACF Remodeling Enzyme Complex (Catalog No. 15-1013) using ACF Remodeling Assay Buffer with or without ATP (50 μM). Curves denote FRET efficiency/chromatin remodeling.<br />\n \n <strong>(Click image to enlarge) </strong></span></span> </p></td>\n \n </tr>\n \n </tbody>\n \n </table>\n \n \n \n \n <table width=\"100%\" border=\"0\" cellspacing=\"3\" cellpadding=\"3\">\n \n <tbody>\n \n <tr>\n \n <td align=\"center\"></td>\n \n </tr>\n \n <tr>\n \n <td align=\"left\" valign=\"top\"></td>\n \n </tr>\n \n </tbody>\n \n </table>\n \n </td>\n \n </tr>\n \n </tbody>\n\n</table>\n\n\n\n\n</body>\n</html>\n","detail_messages":"","gift_wrapping_available":false,"gtin":null,"id":"623","images":[{"alt":"5x ACF Remodeling Assay Buffer","data":"https://cdn11.bigcommerce.com/s-y9o92/images/stencil/{:size}/products/623/598/Untitled__68407.1556128753.png?c=2"}],"main_image":{"alt":"5x ACF Remodeling Assay Buffer","data":"https://cdn11.bigcommerce.com/s-y9o92/images/stencil/{:size}/products/623/598/Untitled__68407.1556128753.png?c=2"},"max_purchase_quantity":0,"meta_description":"5x ACF Remodeling Assay Buffer, ACF Remodeling Assay Buffer, Remodeling Assay Buffer,ACF Remodeling Enzyme Complex, ACF, Remodeling Enzyme Complex, chromatin remodeling complex, EpiDyne control, EpiDyne, ","meta_keywords":"5x ACF Remodeling Assay Buffer, ACF Remodeling Assay Buffer, Remodeling Assay Buffer,ACF Remodeling Enzyme Complex, ACF, Remodeling Enzyme Complex, chromatin remodeling complex, EpiDyne control, EpiDyne, ","min_purchase_quantity":0,"mpn":null,"num_reviews":0,"options":[],"page_title":"5x ACF Remodeling Assay Buffer","price":{"tax_label":"Sales Tax","without_tax":{"currency":"USD","formatted":"$55.00","value":55}},"rating":0,"related_products":[{"add_to_cart_url":"https://www.epicypher.com/cart.php?action=add&product_id=509","add_to_wishlist_url":"/wishlist.php?action=add&product_id=509","availability":"","brand":{"name":null},"category":[],"custom_fields":null,"date_added":"10th Jan 2018","demo":false,"has_options":false,"id":509,"image":{"alt":"Chromatin Remodeling HTS assays","data":"https://cdn11.bigcommerce.com/s-y9o92/images/stencil/{:size}/products/509/350/4_SERVICES_EpiCypher_Blue__31998.1516390300.png?c=2"},"images":[{"alt":"Chromatin Remodeling HTS assays","data":"https://cdn11.bigcommerce.com/s-y9o92/images/stencil/{:size}/products/509/350/4_SERVICES_EpiCypher_Blue__31998.1516390300.png?c=2"}],"low_stock_level":null,"name":"Chromatin Remodeling HTS assays","num_reviews":null,"pre_order":false,"price":{"tax_label":"Sales Tax","without_tax":{"currency":"USD","formatted":"$3.00","value":3}},"qty_in_cart":0,"rating":null,"show_cart_action":true,"sku":null,"stock_level":null,"summary":"Chromatin remodeling regulates DNA access on chromatin and thus impacts gene expression and genome repair. Many ATP-dependent chromatin remodeling enzymes are associated with human disease but are...","url":"https://www.epicypher.com/products/featured-products/chromatin-remodeling-hts-assays","weight":{"formatted":"3.00 LBS","value":3}},{"add_to_cart_url":"https://www.epicypher.com/cart.php?action=add&product_id=551","add_to_wishlist_url":"/wishlist.php?action=add&product_id=551","availability":"","brand":{"name":null},"category":["Nucleosomes/EpiDyne® Chromatin Remodeling Substrates/EpiDyne<sup>®</sup> Modifying Enzymes ","Recombinant Proteins/EpiDyne<sup>®</sup> Chromatin Remodeling Enzymes","Recombinant Proteins/Modifying Enzymes"],"custom_fields":[{"id":585,"name":"Pack Size","value":"100 reactions"}],"date_added":"24th Jul 2018","demo":false,"has_options":false,"id":551,"image":{"alt":"ACF Chromatin Remodeling Enzyme Complex","data":"https://cdn11.bigcommerce.com/s-y9o92/images/stencil/{:size}/products/551/597/Untitled__22497.1556128753.png?c=2"},"images":[{"alt":"ACF Chromatin Remodeling Enzyme Complex","data":"https://cdn11.bigcommerce.com/s-y9o92/images/stencil/{:size}/products/551/597/Untitled__22497.1556128753.png?c=2"}],"low_stock_level":null,"name":"ACF Chromatin Remodeling Enzyme Complex","num_reviews":null,"pre_order":false,"price":{"tax_label":"Sales Tax","without_tax":{"currency":"USD","formatted":"$505.00","value":505}},"qty_in_cart":0,"rating":null,"show_cart_action":true,"sku":"15-1013","stock_level":null,"summary":"\n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n ...","url":"https://www.epicypher.com/products/nucleosomes/epidyne-chromatin-remodeling-substrates/epidyne-modifying-enzymes/acf-chromatin-remodeling-enzyme-complex","weight":{"formatted":"0.01 LBS","value":0.01}},{"add_to_wishlist_url":"/wishlist.php?action=add&product_id=386","availability":"","brand":{"name":null},"category":["Nucleosomes","Nucleosomes/EpiDyne® Chromatin Remodeling Substrates"],"custom_fields":[{"id":601,"name":"Pack Size","value":"50 µg"}],"date_added":"28th Aug 2017","demo":false,"has_options":false,"id":386,"image":{"alt":"EpiDyne-FRET Nucleosome Remodeling Assay Substrate","data":"https://cdn11.bigcommerce.com/s-y9o92/images/stencil/{:size}/products/386/276/2_EpiDyne__20008.1569012494.png?c=2"},"images":[{"alt":"EpiDyne-FRET Nucleosome Remodeling Assay Substrate","data":"https://cdn11.bigcommerce.com/s-y9o92/images/stencil/{:size}/products/386/276/2_EpiDyne__20008.1569012494.png?c=2"}],"low_stock_level":null,"name":"EpiDyne-FRET Nucleosome Remodeling Assay Substrate","num_reviews":null,"out_of_stock_message":false,"pre_order":false,"price":{"tax_label":"Sales Tax","without_tax":{"currency":"USD","formatted":"$595.00","value":595}},"qty_in_cart":0,"rating":null,"show_cart_action":true,"sku":"16-4201","stock_level":null,"summary":" \n\n \n\n \n\n\n\n EpiDyne-FRET Nucleosome Remodeling Assay Substrate Description: Mononucleosomes assembled from recombinant human histones expressed in E. coli (two each of histones H2A-Cy5, H2B,...","url":"https://www.epicypher.com/products/nucleosomes/epidyne-fret-nucleosome-remodeling-assay-substrate","weight":{"formatted":"0.00 LBS","value":0}},{"add_to_cart_url":"https://www.epicypher.com/cart.php?action=add&product_id=373","add_to_wishlist_url":"/wishlist.php?action=add&product_id=373","availability":"","brand":{"name":null},"category":["Nucleosomes/EpiDyne® Chromatin Remodeling Substrates","Nucleosomes/Nucleosome Components and Subunits"],"custom_fields":[{"id":602,"name":"Pack Size","value":"50 µg"}],"date_added":"5th Jul 2017","demo":false,"has_options":false,"id":373,"image":{"alt":"EpiDyne® Remodeling Assay Substrate DNA ST601-GATC0","data":"https://cdn11.bigcommerce.com/s-y9o92/images/stencil/{:size}/products/373/291/DNA__13746.1569012501.gif?c=2"},"images":[{"alt":"EpiDyne® Remodeling Assay Substrate DNA ST601-GATC0","data":"https://cdn11.bigcommerce.com/s-y9o92/images/stencil/{:size}/products/373/291/DNA__13746.1569012501.gif?c=2"}],"low_stock_level":null,"name":"EpiDyne® Remodeling Assay Substrate DNA ST601-GATC0","num_reviews":null,"pre_order":false,"price":{"tax_label":"Sales Tax","without_tax":{"currency":"USD","formatted":"$115.00","value":115}},"qty_in_cart":0,"rating":null,"show_cart_action":true,"sku":"18-4100","stock_level":null,"summary":" EpiDyne Remodeling Assay Substrate DNA ST601-GATC0 Description: EpiDyne Remodeling Assay Substrate DNA ST601-GATC0 is a 217 base-pair double-stranded DNA fragment. This sequence includes the...","url":"https://www.epicypher.com/products/nucleosomes/epidyne-chromatin-remodeling-substrates/epidyne-remodeling-assay-substrate-dna-st601-gatc0","weight":{"formatted":"0.01 LBS","value":0.01}},{"add_to_cart_url":"https://www.epicypher.com/cart.php?action=add&product_id=374","add_to_wishlist_url":"/wishlist.php?action=add&product_id=374","availability":"","brand":{"name":null},"category":["Nucleosomes/EpiDyne® Chromatin Remodeling Substrates","Nucleosomes/Nucleosome Components and Subunits"],"custom_fields":[{"id":637,"name":"Pack Size","value":"50 µg"}],"date_added":"5th Jul 2017","demo":false,"has_options":false,"id":374,"image":{"alt":"EpiDyne® Remodeling Assay Substrate DNA ST601-GATC1","data":"https://cdn11.bigcommerce.com/s-y9o92/images/stencil/{:size}/products/374/292/DNA__93069.1614637188.gif?c=2"},"images":[{"alt":"EpiDyne® Remodeling Assay Substrate DNA ST601-GATC1","data":"https://cdn11.bigcommerce.com/s-y9o92/images/stencil/{:size}/products/374/292/DNA__93069.1614637188.gif?c=2"}],"low_stock_level":null,"name":"EpiDyne® Remodeling Assay Substrate DNA ST601-GATC1","num_reviews":null,"pre_order":false,"price":{"tax_label":"Sales Tax","without_tax":{"currency":"USD","formatted":"$115.00","value":115}},"qty_in_cart":0,"rating":null,"show_cart_action":true,"sku":"18-4101","stock_level":null,"summary":" EpiDyne Remodeling Assay Substrate DNA ST601-GATC1 Description: EpiDyne Remodeling Assay Substrate DNA ST601-GATC1 is a 217 base-pair double-stranded DNA fragment. This sequence includes the...","url":"https://www.epicypher.com/products/nucleosomes/epidyne-chromatin-remodeling-substrates/epidyne-remodeling-assay-substrate-dna-st601-gatc1","weight":{"formatted":"0.01 LBS","value":0.01}}],"shipping":{"calculated":true},"shipping_messages":[],"show_quantity_input":1,"sku":"21-0013","tags":[],"title":"5x ACF Remodeling Assay Buffer","upc":null,"url":"https://www.epicypher.com/products/recombinant-proteins/modifying-enzymes/5x-acf-remodeling-assay-buffer","warranty":"","weight":"0.01 LBS"}
Pack Size: 500 µL
