SKU: 21-0013
{"url":"https://www.epicypher.com/products/recombinant-proteins/modifying-enzymes/5x-acf-remodeling-assay-buffer","add_this":[{"service":"facebook","annotation":""},{"service":"email","annotation":""},{"service":"print","annotation":""},{"service":"twitter","annotation":""},{"service":"linkedin","annotation":""}],"gtin":"","id":"623","bulk_discount_rates":[],"can_purchase":true,"meta_description":"5x ACF Remodeling Assay Buffer, ACF Remodeling Assay Buffer, Remodeling Assay Buffer,ACF Remodeling Enzyme Complex, ACF, Remodeling Enzyme Complex, chromatin remodeling complex, EpiDyne control, EpiDyne, ","category":["Recombinant Proteins/Modifying Enzymes"],"AddThisServiceButtonMeta":"","main_image":{"data":"https://cdn11.bigcommerce.com/s-y9o92/images/stencil/{:size}/products/623/598/Untitled__68407.1556128753.png?c=2","alt":"5x ACF Remodeling Assay Buffer"},"add_to_wishlist_url":"/wishlist.php?action=add&product_id=623","shipping":{"calculated":true},"num_reviews":0,"weight":"0.01 LBS","custom_fields":[{"id":"587","name":"Pack Size","value":"500 µL"}],"sku":"21-0013","description":"<table width=\"100%\" border=\"0\" cellspacing=\"2\" cellpadding=\"2\">\n \n <tbody>\n \n <tr valign=\"top\">\n \n <td>\n \n \n \n <table width=\"95%\" border=\"0\" cellspacing=\"1\" cellpadding=\"1\">\n \n <tbody>\n \n <tr>\n \n <td>\n \n <table width=\"100%\" border=\"0\" cellspacing=\"0\" cellpadding=\"0\">\n \n <tbody>\n \n <tr></tr>\n \n </tbody>\n \n </table>\n \n </td>\n \n </tr>\n \n <tr>\n \n <td valign=\"top\"><span style=\"font-family: Arial; \" class=\"Apple-style-span\"><span style=\"font-size: 12pt; \" class=\"Apple-style-span\"><b><span class=\"Apple-style-span\" style=\"font-family: Arial; \"><span class=\"Apple-style-span\" style=\"font-size: 12pt; \"><b>5x ACF Remodeling Assay Buffer<br>\n </b></span></span> Description:</b> 5x concentrated reaction buffer for use with ACF Remodeling Enzyme Complex (Catalog No. 15-1013). Optimized for use with recombinant Drosophila ACF Remodeling Enzyme Complex, assembled from Acf1-FLAG and ISWI produced in SF9 cells. ACF is an ATP-dependent chromatin remodeling complex that regulates nucleosome spacing.</span></span>\n \n </td>\n \n </tr>\n \n <tr>\n \n <td valign=\"top\"><span style=\"font-family: Arial; \" class=\"Apple-style-span\"><span style=\"font-size: 12pt; \" class=\"Apple-style-span\"><b><span class=\"Apple-style-span\" style=\"font-family: Arial; \"><span class=\"Apple-style-span\" style=\"font-size: 12pt; \"><b>ACF Remodeling Enzyme Complex</b></span></span> Formulation:</b> 5x ACF Remodeling Assay Buffer (100 mM Tris HCl, pH 7.5, 250 mM KCl, 15 mM MgCl2, 0.05% (w/v) BSA, 0.05% (v/v) Tween 20) is formulated for use with ACF Remodeling Enzyme Complex.<br />\n \n <br />\n \n </span></span>\n \n </td>\n \n </tr>\n \n <tr>\n \n <td valign=\"top\"><span style=\"font-family: Arial; \" class=\"Apple-style-span\"><span style=\"font-size: 12pt; \" class=\"Apple-style-span\"><b>Storage and Stability:</b> Stable for six months at 4°C from date of receipt.<br />\n \n <br />\n \n </span></span>\n \n </td>\n \n </tr>\n \n <tr>\n \n <td valign=\"top\"><p><span style=\"font-family: Arial; \" class=\"Apple-style-span\"><span style=\"font-size: 12pt; \" class=\"Apple-style-span\"><b>Application Notes:</b> This produt is formulated to perform remodeling reactions using ACF Remodeling Enzyme Complex and EpiDyne®-FRET substrate in a reaction defined as 10 μl containing [5 nM ACF Complex/ 20 nM EpiDyne®-FRET/ 50 μM rATP] and remodels to completion in <20 minutes. For best results, store 5x ACF Remodeling Assay buffer at 4ºC and avoid freezing.</span></span></p>\n <p><span style=\"font-family: Arial; \" class=\"Apple-style-span\"><span style=\"font-size: 12pt; \" class=\"Apple-style-span\"> For further information, please contact techsupport@epicypher.com or refer to the EpiDyne®- FRET Technote (TN#106; available at www.epicypher.com/technotes).</span></span><span style=\"font-family: Arial; \" class=\"Apple-style-span\"><span style=\"font-size: 12pt; \" class=\"Apple-style-span\"><br />\n \n </span></span></p>\n \n </td>\n \n </tr>\n \n <tr>\n \n <td valign=\"top\"><p><span style=\"font-family: Arial; \" class=\"Apple-style-span\"><span style=\"font-size: 12pt; \" class=\"Apple-style-span\"><br />\n \n </span></span><br />\n \n </p>\n \n </td>\n \n </tr>\n \n <tr>\n \n <td valign=\"top\"><span style=\"font-family: Arial; \" class=\"Apple-style-span\"><span style=\"font-size: 12pt; \" class=\"Apple-style-span\">View technical datasheet for this product. <a href=\"https://www.epicypher.com/content/documents/tds/21-0013.pdf\" target=\"_blank\"><img src=\"https://cdn7.bigcommerce.com/s-y9o92/content/documents/tds/icon.png\" alt=\"21-0013 Datasheet\" width=\"30\" height=\"40\" /></a></span></span>\n \n </td>\n \n </tr>\n \n </tbody>\n \n </table>\n \n </td>\n \n <td>\n \n <table width=\"100%\" border=\"0\" cellspacing=\"3\" cellpadding=\"3\">\n \n <tbody>\n \n <tr>\n \n <td align=\"center\"><a href=\"https://www.epicypher.com/content/images/products/proteins/15_1013_ATP_dep_chromatin_remodeling.jpg\" target=\"_new\">\n \n <img src=\"https://cdn7.bigcommerce.com/s-y9o92/content/images/products/proteins/15_1013_ATP_dep_chromatin_remodeling.jpg\" alt=\"15-1013 ATP-dependent Chromatin Remodeling \" width=\"250\" height=\"183\" /></a>\n \n </td>\n \n </tr>\n \n <tr>\n \n <td align=\"left\" valign=\"top\">\n \n <p><span style=\"font-family: Arial; \" class=\"Apple-style-span\"><span style=\"font-size: 10pt; \" class=\"Apple-style-span\"><strong>ATP-dependent Chromatin Remodeling Assay:</strong> EpiDyne®- FRET Chromatin Remodeling Substrate (20 nM; Catalog No. 16-4201) incubated with ACF Remodeling Enzyme Complex (Catalog No. 15-1013) using ACF Remodeling Assay Buffer with or without ATP (50 μM). Curves denote FRET efficiency/chromatin remodeling.<br />\n \n <strong>(Click image to enlarge) </strong></span></span> </p></td>\n \n </tr>\n \n </tbody>\n \n </table>\n \n \n \n \n <table width=\"100%\" border=\"0\" cellspacing=\"3\" cellpadding=\"3\">\n \n <tbody>\n \n <tr>\n \n <td align=\"center\"></td>\n \n </tr>\n \n <tr>\n \n <td align=\"left\" valign=\"top\"></td>\n \n </tr>\n \n </tbody>\n \n </table>\n \n </td>\n \n </tr>\n \n </tbody>\n\n</table>\n\n\n\n\n</body>\n</html>\n","tags":[],"warranty":"","price":{"without_tax":{"formatted":"$55.00","value":55,"currency":"USD"},"tax_label":"Sales Tax"},"detail_messages":"","availability":"","page_title":"5x ACF Remodeling Assay Buffer","cart_url":"https://www.epicypher.com/cart.php","max_purchase_quantity":0,"mpn":"","upc":null,"options":[],"related_products":[{"id":876,"sku":"21-0014","name":"5x SMARCA Remodeling Assay 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Pack Size: 500 µL
