Histone H3.3 aa75-94 Peptide, Biotinylated Description:A synthetic peptide derived from human H3.3, amino acids 75-94. The alpha-amino group on the N-terminus is acetylated, there is a PEG spacer, an added lysine residue containing a biotin moiety at the epsilonamino group, and a C-terminal amide group (CONH2).
Purity: >85% as judged by HPLC and mass spectrometry.
Storage and Stability:Lyophilized peptides are stable for 2 years at -20°C from date of shipment. Peptides in solution are stable for 6 months at -80°C. Spin vial briefly in microfuge and reconstitute with sterile distilled water and vortex briefly until resuspended. Aliquot peptide to avoid repeated freezing and thawing.
Quality Control: Peptide purified by reversed-phase HPLC and analyzed using MALDI-TOF mass spectrometry and analytical RP-HPLC.
Mass Spec Data: MALDI-TOF mass spectrometry was used to analyze histone H3.3 aa75-94 peptide for identity and homogeneity.