Histone H4K5, K8ac, K12me1, K16ac Peptide, Biotinylated Description: A synthetic peptide derived from human histone H4, amino acids 1-23, acetylated at lysines 5, 8, and 16 and monomethylated at lysine 12. The alpha-amino group on the N-terminus is acetylated, with a C-terminal PEG spacer containing a biotin moiety.
Purity: >97% as judged by HPLC and mass spectrometry.
Storage and Stability: Lyophilized peptides are stable for 2 years at -20°C from date of shipment. Peptides in solution are stable for 6 months at -80°C. Spin vial briefly in microfuge and reconstitute with sterile distilled water and vortex briefly until resuspended. Aliquot peptide to avoid repeated freezing and thawing.
Quality Control: Peptide purified by reversed-phase HPLC and analyzed using MALDI-TOF mass spectrometry and analytical RP-HPLC.
Mass Spec Data: MALDI-TOF mass spectrometry was used to analyze Histone H4K5, K8ac, K12me1, K16ac Peptide, for identity and homogeneity.