Anti-Rabbit

Anti-Rabbit Secondary Antibody for CUTANA™ ChIC/CUT&Tag Workflows

$50.00
SKU: 13-0047
Pack Size: 50 reactions
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Type:  Recombinant Polyclonal Host:  Goat
Mol Wgt.:  N/A Appl.:  ChIC/CUT&Tag
Format:  Aff. Pur. IgG Reactivity: Anti-Rabbit
Anti-Rabbit Secondary Antibody for CUTANA™ ChIC/CUT&Tag Workflows Description: Anti-Rabbit Secondary Antibody for CUTANA™ ChIC/CUT&Tag Workflows is an affinity-purified and specific for rabbit immunoglobulins. Minimal cross reactivity with mouse, rat, human, bovine, guinea pig, and donkey IgG is observed. Use of a secondary antibody in ChIC/CUT&Tag aids in signal amplification by increasing bound IgG per target epitope. For best results, use with CUTANA™ pAG-Tn5 (EpiCypher Catalog No. 15-1017) and CUTANA Compatible Antibodies.
Anti-Rabbit Secondary Antibody for CUTANA™ ChIC/CUT&Tag Workflows Immunogen: Recombinant full-length rabbit immunoglobulin protein.

Anti-Rabbit Secondary Antibody for CUTANA™ ChIC/CUT&Tag Workflows Formulation: Protein G affinity-purified antibody (1 mg/mL) in PBS, pH 7.2, with 0.09% sodium azide.

Anti-Rabbit Secondary Antibody for CUTANA™ ChIC/CUT&Tag Workflows Storage and Stability: Store at 4ºC short term. For long term storage, store at -20ºC. Avoid freeze/thaw cycles.

Anti-Rabbit Secondary Antibody for CUTANA™ ChIC/CUT&Tag Workflows Application Notes
Recommended usage amounts:
 
ChIC/CUT&Tag: 0.5 μg per sample

For detailed instructions, see EpiCypher CUTANA Direct-to-PCR CUT&Tag protocol: EpiCypher.com/resources/protocols

Anti-Rabbit Secondary Antibody for CUTANA™ ChIC/CUT&Tag Workflows References:

View technical datasheet for this product. 13-0047 Datasheet

Applications Key: ChIP-Chromatin IP; E-ELISA; FACS-Flow cytometry; IF-Immunofluorescence; IHC-Immunohistochemistry; ICC-Immunocytochemistry; L-Luminex; IP-Immunoprecipitation; WB-Western Blotting

Reactivity Key: B-Bovine; Ce-C. elegans; Ch-Chicken; Dm- Drosophila; Eu-Eukaryote; H-Human; M-Mouse; Ma-Mammal; R-Rat; Sc-S.cerevesiae; Sp-S. pombe; WR-Wide Range (predicted); X-Xenopus; Z-Zebrafish

13-0047 CUT&Tag Data

CUT&Tag Data:Representative sequencing track obtained using Anti-Rabbit Secondary Antibody for CUTANA™ ChIC/CUT&Tag Workflows (0.5 μg) is shown at a 324 kb window centered at the LAMC3 gene. CUT&Tag was performed using the EpiCypher CUTANA Direct-to-PCR CUT&Tag protocol EpiCypher.com/resources/protocols using 100,000 K-562 nuclei with rabbit anti-H3K27me3 antibody (EpiCypher Catalog No. 13-0030, Lot 18303001). Image was generated using the Integrative Genomics Viewer (IGV, Broad Institute). Anti-Rabbit Secondary Antibody for CUTANA™ ChIC/CUT&Tag Workflows produced clear peaks with a genomic distribution pattern consistent with H3K27me3.
(Click image to enlarge)

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