SNF2L/SMARCA1

SNF2L/SMARCA1 CUTANA™ CUT&RUN Antibody

$445.00
SKU: 13-2005
Pack Size: 100 µL
  • Type:  Polyclonal
  • Target Size:  123 kDa
  • Format:  Aff. Pur. IgG
  • Host:  Rabbit
  • Appl.:  CUT&RUN, IP, IHC
  • Reactivity: Human (predicted: M)

Description

This antibody meets EpiCypher’s “CUTANA Compatible” criteria for performance in Cleavage Under Targets and Release Using Nuclease (CUT&RUN) and/or Cleavage Under Targets and Tagmentation (CUT&Tag) approaches to genomic mapping. Every lot of a CUTANA Compatible antibody is tested in the indicated CUTANA approach using EpiCypher optimized protocols (EpiCypher.com/resources/protocols) and determined to yield peaks that show a genomic distribution pattern consistent with reported function(s) of the target protein. SNF2L antibody produces CUT&RUN peaks above background (Figure 1) that overlap with H3K4me3 (Figures 1-2), consistent with its known role as the ATP-dependent helicase subunit of the NURF ISWI chromatin remodeler complex (1).

Validation Data

Figure 1: SNF2L enrichment at annotated transcription start sites (TSSs) in CUT&RUN.
CUT&RUN was performed using 500,000 K562 cells with SNF2L and control antibodies (0.5 µg each; IgG, EpiCypher 13-0042; H3K4me3, EpiCypher 13-0041). Sequencing reads were aligned to TSSs (+/- 2 kbp) of 18,793 genes. Signal (red) over background (blue) is ranked by intensity (top to bottom). All rows aligned to SNF2L antibody with moderate fixation (0.1% formaldehyde, 1 min), which improved signal vs. native conditions.

Figure 2: SNF2L CUT&RUN peaks and functional overlap.
Two representative gene loci from the CUT&RUN data in Figure 1 are shown. SNF2L enrichment overlaps with H3K4me3 peaks, consistent with its reported function as a member of the NURF ISWI chromatin remodeler complex (1). Improved signal recovery with moderate fixation (0.1% formaldehyde, 1 min), is notable. Images generated in Integrative Genomics Viewer (Broad Institute).

Figure 3: Immunoprecipitation of human SNF2L.
EpiCypher SNF2L antibody (6 µg) was used to immunoprecipitate whole cell lysates isolated from HeLa cells using NETN lysis buffer (0.5 - 1.0 mg per IP). A negative control IgG antibody and positive control SNF2L antibody (Bethyl Laboratories) were also used for comparison. Immunoprecipitates were loaded onto 4-8% SDS-PAGE gel (20% of IP loaded) and probed via western blot with EpiCypher SNF2L antibody (1 µg/mL).

Figure 4: Immunohistochemistry detection of human SNF2L.
FFPE section of human ovarian carcinoma examined using SNF2L antibody (1:1,000 dilution, 1 µg/mL).

Technical Information

Immunogen
A synthetic peptide corresponding to human SNF2L amino acids 1004 to 1054.
Formulation
Antigen affinity-purified antibody (1 mg/mL) in Tris-citrate/phosphate buffer pH 7 to 8, 0.09% sodium azide.
Storage and Stability
Stable for 1 year at 4°C from date of receipt.

Application Notes

Recommended Dilutions:
CUT&RUN: 0.5 µg
IP: 2 - 10 µg/mg lysate
IHC: 1:1000 - 1:5,000*

Additional Info

This product is provided for commercial sale under license from Bethyl Laboratories, Inc.


Applications Key:

ChIP: Chromatin immunoprecipitation
CUT&RUN: Cleavage Under Targets and Release Using Nuclease
CUT&Tag: Cleavage Under Targets and Tagmentation
E: ELISA
FACS: Flow cytometry
ICC: Immunocytochemistry
IF: Immunofluorescence
IHC: Immunohistochemistry
IP: Immunoprecipitation
L: Luminex
WB: Western Blot

Reactivity Key:

B-Bovine
Ce-C. elegans
Ch-Chicken
Dm- Drosophila
Eu-Eukaryote
H-Human
M-Mouse
Ma-Mammal
R-Rat
Sc-S.cerevesiae
Sp-S.pombe
WR-Wide Range (predicted)
X-Xenopus
Z-Zebrafish
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