FOXA1/HNF3A

FOXA1/HNF3A CUTANA™ CUT&RUN Antibody

$445.00
SKU: 13-2001
Pack size: 100 µL
Type:  Polyclonal Host:  Rabbit
Target Size:  49 kDa Appl.:  CUT&RUN, WB, IP
Format:  Aff. Pur. IgG Reactivity: H, M (predicted: rat)
FOXA1/HNF3A CUTANA™ CUT&RUN Antibody Description: This antibody meets EpiCypher’s "CUTANA Compatible" criteria for performance in Cleavage Under Targets and Release Using Nuclease (CUT&RUN) and/or Cleavage Under Targets and Tagmentation (CUT&Tag) approaches to genomic mapping. Every lot of a CUTANA Compatible antibody is tested in the indicated CUTANA approach using EpiCypher optimized protocols (EpiCypher.com/resources/protocols) and determined to yield peaks that show a genomic distribution pattern consistent with reported function(s) of the target protein. FOXA1 antibody produces CUT&RUN peaks above background primarily in intronic, intergenic, and promoter regions (Figure 1) that overlap with known FOXA1 DNA-binding motifs (Figure 2).

FOXA1/HNF3A CUTANA™ CUT&RUN Antibody Immunogen: A synthetic peptide corresponding to human FOXA1 amino acids 422 to 472.

FOXA1/HNF3A CUTANA™ CUT&RUN Antibody Formulation: Antigen affinity-purified antibody (1.0 mg/mL) in Tris-citrate/phosphate buffer pH 7 to 8, 0.09% sodium azide.

FOXA1/HNF3A CUTANA™ CUT&RUN Antibody Storage and Stability: Stable for 1 year at 4°C from date of receipt.

FOXA1/HNF3A CUTANA™ CUT&RUN Antibody Application Notes
Recommended Dilutions:
CUT&RUN: 0.5 µg
WB: 1:1,000 - 1:10,000
IP: 2 - 10 µg/mg lysate

FOXA1/HNF3A CUTANA™ CUT&RUN Antibody References:

This product is provided for commercial sale under license from Bethyl Laboratories, Inc.

View technical datasheet for this product. 13-2001 Datasheet

Applications Key: ChIP-Chromatin IP; CUT&RUN: Cleavage Under Targets and Release Using Nuclease; CUT&Tag: Cleavage Under Targets and Tagmentation; E-ELISA; FACS-Flow cytometry; IF-Immunofluorescence; IHC-Immunohistochemistry; ICC-Immunocytochemistry; L-Luminex; IP-Immunoprecipitation; WB-Western Blotting

Reactivity Key: B-Bovine; Ce-C. elegans; Ch-Chicken; Dm- Drosophila; Eu-Eukaryote; H-Human; M-Mouse; Ma-Mammal; R-Rat; Sc-S.cerevesiae; Sp-S. pombe; WR-Wide Range (predicted); X-Xenopus; Z-Zebrafish

13-2001 Heatmap

Figure 1: FOXA1 peaks in CUT&RUN. CUT&RUN was performed using 500,000 MCF7 cells with 0.5 µg FOXA1 antibody. Peaks were called using MACS2. (A) Heatmap showing FOXA1 peaks relative to IgG negative control antibody (EpiCypher 13-0042) in aligned rows ranked by intensity (top to bottom) and colored such that red indicates high localized enrichment and blue denotes background signal. (B) The number of peaks which fall into distinct classes of functionally annotated genomic regions is plotted.
(Click image to enlarge)

13-2001 Motif Analysis

Figure 2: FOXA1 transcription factor binding motif analysis in CUT&RUN. (A) Homer software was used to identify known motifs underneath FOXA1 CUT&RUN peaks. The FOXA1 consensus motif was highly enriched, and is represented as a sequence logo position weight matrix. (B) The number of FOXA1 peaks containing FOXA1 consensus motifs from panel A is represented by a Venn Diagram. (C) Three representative loci showing FOXA1 peaks overlapping with FOXA1 consensus motifs (Integrative Genomics Viewer).
(Click image to enlarge)

13-2001 Western Blot

Figure 3: Western blot detection of human and mouse FOXA1. Whole cell lysates were isolated from MCF-7 and HeLa cells using NETN lysis buffer. The indicated amounts (µg) of lysate were loaded onto 4-20% SDS-PAGE gel and analyzed under standard western blot conditions using FOXA1 antibody (0.1 µg/mL).
(Click image to enlarge)

13-2001 IP

Figure 4: Immunoprecipitation of human FOXA1. EpiCypher FOXA1 antibody (6 µg) was used to immunoprecipitate whole cell lysates isolated from HeLa cells using NETN lysis buffer (1 mg per IP). A negative control IgG antibody and positive control antibody to a different FOXA1 epitope (Bethyl Laboratories) were also used to demonstrate specificity of the IP. Immunoprecipitates were loaded onto 4-20% SDS-PAGE gel (20% of IP loaded) and probed via western blot with EpiCypher FOXA1 antibody (1 µg/mL).
(Click image to enlarge)

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