

FOXA1/HNF3A CUTANA™ CUT&RUN Antibody
{"url":"https://www.epicypher.com/products/antibodies/foxa1-hnf3a-cutana-cut-run-antibody","add_this":[{"service":"facebook","annotation":""},{"service":"email","annotation":""},{"service":"print","annotation":""},{"service":"twitter","annotation":""},{"service":"linkedin","annotation":""}],"gtin":null,"id":"796","bulk_discount_rates":[],"can_purchase":true,"meta_description":"Rabbit polyclonal anti-FOXA1/HNF3A antibody validated for CUT&RUN, WB, IP. Rigorously tested for reliable and robust performance in CUT&RUN assays.","category":["Antibodies","Antibodies/CUTANA™ CUT&RUN Antibodies","Antibodies/CUTANA™ CUT&RUN Antibodies/CUT&RUN Antibodies - Chromatin-Associated Proteins","Epigenetics Kits and Reagents/CUTANA™ ChIC / CUT&RUN Assays"],"AddThisServiceButtonMeta":"","main_image":{"data":"https://cdn11.bigcommerce.com/s-y9o92/images/stencil/{:size}/products/796/787/2020.12.08_CUTANA_ChAPs_Antibody_thumbnail_RGB_LS__81112.1607995124.png?c=2","alt":"FOXA1/HNF3A CUTANA™ CUT&RUN Antibody"},"add_to_wishlist_url":"/wishlist.php?action=add&product_id=796","shipping":{"calculated":true},"num_reviews":0,"weight":"0.01 LBS","custom_fields":[{"id":"654","name":"Pack size","value":"100 µL"}],"sku":"13-2001","description":"<div class=\"product-general-info\">\n <ul class=\"product-general-info__list-left\">\n <li class=\"product-general-info__list-item\">\n <strong>Type: </strong>Polyclonal\n </li>\n <li class=\"product-general-info__list-item\">\n <strong>Host: </strong>Rabbit\n </li>\n <li class=\"product-general-info__list-item\">\n <strong>Applications: </strong>CUT&RUN, WB, IP\n </li>\n </ul>\n <ul class=\"product-general-info__list-right\">\n <li class=\"product-general-info__list-item\">\n <strong>Reactivity: </strong>Human, Mouse (Predicted Rat)\n </li>\n <li class=\"product-general-info__list-item\">\n <strong>Format: </strong>Antigen affinity-purified\n </li>\n <li class=\"product-general-info__list-item\">\n <strong>Target Size: </strong>49 kDa\n </li>\n </ul>\n</div>\n<div class=\"service_accordion product-droppdown\">\n <div class=\"container\">\n <div id=\"prodAccordion\">\n <div id=\"ProductDescription\" class=\"Block Panel current\">\n <h3 class=\"sub-title1\">Description</h3>\n <div\n class=\"ProductDescriptionContainer product-droppdown__section-description-specific\">\n <p>\n This antibody meets EpiCypher’s “CUTANA Compatible” criteria for\n performance in Cleavage Under Targets and Release Using Nuclease\n (CUT&RUN) and/or Cleavage Under Targets and Tagmentation\n (CUT&Tag) approaches to genomic mapping. Every lot of a CUTANA\n Compatible antibody is tested in the indicated approach using\n EpiCypher optimized\n <a href=\"/resources/protocols\" target=\"_blank\">protocols</a>\n and determined to yield peaks that show a genomic distribution\n pattern consistent with reported function(s) of the target protein.\n FOXA1 antibody produces CUT&RUN peaks above background primarily in\n intronic, intergenic, and promoter regions\n <strong>(Figure 1)</strong> that overlap with known FOXA1\n DNA-binding motifs <strong>(Figure 2)</strong>.\n </p>\n </div>\n </div>\n </div>\n <div id=\"prodAccordion\">\n <div id=\"ProductDescription\" class=\"Block Panel current\">\n <h3 class=\"sub-title1\">Validation Data</h3>\n <div\n class=\"ProductDescriptionContainer product-droppdown__section-description-specific\">\n <p>\n CUT&RUN was performed on 500k MCF7 cells with 0.5 µg of either\n FOXA1/HNF3A or IgG negative control (EpiCypher\n <a\n href=\"/products/nucleosomes/snap-cutana-spike-in-controls/cutana-rabbit-igg-cut-run-negative-control-antibody\"\n target=\"_blank\"\n >13-0042</a\n >) antibodies using the CUTANA™ ChIC/CUT&RUN Kit v2.0 (EpiCypher\n <a\n href=\"/products/epigenetics-reagents-and-assays/cutana-chic-cut-and-run-kit\"\n target=\"_blank\"\n >14-1048</a\n >). Library preparation was performed with 5 ng of DNA (or the total\n amount recovered if less than 5 ng) using the CUTANA™ CUT&RUN\n Library Prep Kit (EpiCypher\n <a\n href=\"/products/epigenetics-reagents-and-assays/cutana-cut-and-run-library-prep-kit\"\n target=\"_blank\"\n >14-1001/14-1002</a\n >). Both kit protocols were adapted for high throughput Tecan liquid\n handling. Libraries were run on an Illumina NextSeq2000 with\n paired-end sequencing (2x50 bp). Sample sequencing depth was 2.5\n million reads (FOXA1) and 2.4 million reads (IgG). Data were aligned\n to the hg19 genome using Bowtie2. Data were filtered to remove\n duplicates, multi-aligned reads, and blacklist regions.\n </p>\n <section class=\"image-picker\">\n <div class=\"image-picker__left\">\n <div\n class=\"image-picker__main-content_active image-picker__main-content\">\n <div class=\"image-picker__header-content\">\n <button class=\"image-picker__left-arrow\">\n <svg\n class=\"image-picker__svg-left\"\n width=\"24\"\n height=\"24\"\n viewBox=\"0 0 24 24\">\n <path\n d=\"M16.67 0l2.83 2.829-9.339 9.175 9.339 9.167-2.83 2.829-12.17-11.996z\" />\n </svg>\n </button>\n <a\n href=\"/content/images/products/antibodies/13_2001_heatmap.jpeg\"\n target=\"_new\"\n class=\"image-picker__main-image-link\"\n ><img loading=\"lazy\"\n alt=\"13_2001_Heatmap\"\n src=\"/content/images/products/antibodies/13_2001_heatmap.jpeg\"\n class=\"image-picker__main-image\" />\n <span class=\"image-picker__main-image-caption\"\n >(Click to enlarge)</span\n ></a\n >\n <button class=\"image-picker__right-arrow\">\n <svg\n class=\"image-picker__svg-right\"\n width=\"24\"\n height=\"24\"\n viewBox=\"0 0 24 24\">\n <path\n d=\"M7.33 24l-2.83-2.829 9.339-9.175-9.339-9.167 2.83-2.829 12.17 11.996z\" />\n </svg>\n </button>\n </div>\n <p>\n <span class=\"image-picker__span-content\"\n ><strong>Figure 1: FOXA1 peaks in CUT&RUN </strong\n ><br />\n CUT&RUN was performed as described above. Peaks were called\n with MACS2. <b>(A)</b> Heatmap showing FOXA1 peaks relative\n to IgG negative control antibody in aligned rows ranked by\n intensity (top to bottom) and colored such that red\n indicates high localized enrichment and blue denotes\n background signal. <b>(B)</b> The number of peaks that fall\n into distinct classes of functionally annotated genomic\n regions are shown.\n </span>\n </p>\n </div>\n <div class=\"image-picker__main-content\">\n <div class=\"image-picker__header-content\">\n <button class=\"image-picker__left-arrow\">\n <svg\n class=\"image-picker__svg-left\"\n width=\"24\"\n height=\"24\"\n viewBox=\"0 0 24 24\">\n <path\n d=\"M16.67 0l2.83 2.829-9.339 9.175 9.339 9.167-2.83 2.829-12.17-11.996z\" />\n </svg>\n </button>\n <a\n href=\"/content/images/products/antibodies/13_2001_tracks.jpeg\"\n target=\"_new\"\n class=\"image-picker__main-image-link\"\n ><img loading=\"lazy\"\n alt=\"13_2001_Tracks\"\n src=\"/content/images/products/antibodies/13_2001_tracks.jpeg\"\n class=\"image-picker__main-image\" />\n <span class=\"image-picker__main-image-caption\"\n >(Click to enlarge)</span\n ></a\n >\n <button class=\"image-picker__right-arrow\">\n <svg\n class=\"image-picker__svg-right\"\n width=\"24\"\n height=\"24\"\n viewBox=\"0 0 24 24\">\n <path\n d=\"M7.33 24l-2.83-2.829 9.339-9.175-9.339-9.167 2.83-2.829 12.17 11.996z\" />\n </svg>\n </button>\n </div>\n <p>\n <span class=\"image-picker__span-content\"\n ><strong\n >Figure 2: FOXA1 transcription factor binding motif\n analysis in CUT&RUN</strong\n ><br />\n (<b>A</b>) Homer analysis determined that the FOXA1\n consensus motif, represented as a sequence logo position\n weight matrix, was enriched under FOXA1 CUT&RUN peaks.\n (<b>B</b>) The number of FOXA1 peaks containing consensus\n motifs from panel A is represented by a Venn Diagram.\n (<b>C</b>) Gene browser shots were generated using the\n Integrative Genomics Viewer (IGV, Broad Institute). Three\n representative loci show overlap of FOXA1 peaks with\n consensus motifs.\n </span>\n </p>\n </div>\n <div class=\"image-picker__main-content\">\n <div class=\"image-picker__header-content\">\n <button class=\"image-picker__left-arrow\">\n <svg\n class=\"image-picker__svg-left\"\n width=\"24\"\n height=\"24\"\n viewBox=\"0 0 24 24\">\n <path\n d=\"M16.67 0l2.83 2.829-9.339 9.175 9.339 9.167-2.83 2.829-12.17-11.996z\" />\n </svg>\n </button>\n <a\n href=\"/content/images/products/antibodies/13_2001_WB.jpeg\"\n target=\"_new\"\n class=\"image-picker__main-image-link\"\n ><img loading=\"lazy\"\n alt=\"13_2001_WB\"\n src=\"/content/images/products/antibodies/13_2001_WB.jpeg\"\n class=\"image-picker__main-image\" />\n <span class=\"image-picker__main-image-caption\"\n >(Click to enlarge)</span\n ></a\n >\n <button class=\"image-picker__right-arrow\">\n <svg\n class=\"image-picker__svg-right\"\n width=\"24\"\n height=\"24\"\n viewBox=\"0 0 24 24\">\n <path\n d=\"M7.33 24l-2.83-2.829 9.339-9.175-9.339-9.167 2.83-2.829 12.17 11.996z\" />\n </svg>\n </button>\n </div>\n <p>\n <span class=\"image-picker__span-content\"\n ><strong>Figure 3: Western blot data</strong><br />\n Western analysis of FOXA1 in whole cell lysates from MCF-7\n and HeLa cells using NETN lysis buffer. The indicated\n amounts (µg) of lysate were loaded onto 4-20% SDS-PAGE gel\n and analyzed under standard western blot conditions using\n FOXA1 antibody (0.1 µg/mL).\n </span>\n </p>\n </div>\n <div class=\"image-picker__main-content\">\n <div class=\"image-picker__header-content\">\n <button class=\"image-picker__left-arrow\">\n <svg\n class=\"image-picker__svg-left\"\n width=\"24\"\n height=\"24\"\n viewBox=\"0 0 24 24\">\n <path\n d=\"M16.67 0l2.83 2.829-9.339 9.175 9.339 9.167-2.83 2.829-12.17-11.996z\" />\n </svg>\n </button>\n <a\n href=\"/content/images/products/antibodies/13_2001_IP.jpeg\"\n target=\"_new\"\n class=\"image-picker__main-image-link\"\n ><img loading=\"lazy\"\n alt=\"13_2012_IP\"\n src=\"/content/images/products/antibodies/13_2001_IP.jpeg\"\n class=\"image-picker__main-image\" />\n <span class=\"image-picker__main-image-caption\"\n >(Click to enlarge)</span\n ></a\n >\n <button class=\"image-picker__right-arrow\">\n <svg\n class=\"image-picker__svg-right\"\n width=\"24\"\n height=\"24\"\n viewBox=\"0 0 24 24\">\n <path\n d=\"M7.33 24l-2.83-2.829 9.339-9.175-9.339-9.167 2.83-2.829 12.17 11.996z\" />\n </svg>\n </button>\n </div>\n <p>\n <span class=\"image-picker__span-content\"\n ><strong\n >Figure 4: Immunoprecipitation of human FOXA1</strong\n ><br />\n EpiCypher FOXA1 antibody (6 µg) was used to\n immunoprecipitate whole cell lysates isolated from HeLa\n cells using NETN lysis buffer (1 mg per IP). A negative\n control IgG antibody and positive control antibody to a\n different FOXA1 epitope (Bethyl Laboratories) were also used\n to demonstrate specificity of the IP. Immunoprecipitates\n were loaded onto 4-20% SDS-PAGE gel (20% of IP loaded) and\n probed via western blot with EpiCypher FOXA1 antibody (1\n µg/mL).\n </span>\n </p>\n </div>\n </div>\n <aside class=\"image-picker__right\">\n <div class=\"image-picker__gallery\">\n <img loading=\"lazy\"\n alt=\"13_2001_heatmap\"\n src=\"/content/images/products/antibodies/13_2001_heatmap.jpeg\"\n width=\"200\"\n class=\"image-picker__side-image image-picker__side-image_active\"\n role=\"button\" />\n <img loading=\"lazy\"\n alt=\"13_2001_tracks\"\n src=\"/content/images/products/antibodies/13_2001_tracks.jpeg\"\n class=\"image-picker__side-image\"\n role=\"button\" />\n <img loading=\"lazy\"\n alt=\"13_2001_WB\"\n src=\"/content/images/products/antibodies/13_2001_WB.jpeg\"\n class=\"image-picker__side-image\"\n role=\"button\" />\n <img loading=\"lazy\"\n alt=\"13_2012_IP\"\n src=\"/content/images/products/antibodies/13_2001_IP.jpeg\"\n class=\"image-picker__side-image\"\n role=\"button\" />\n </div>\n </aside>\n </section>\n </div>\n </div>\n </div>\n <div id=\"prodAccordion\">\n <div id=\"ProductDescription\" class=\"Block Panel\">\n <h3 class=\"sub-title1\">Technical Information</h3>\n <div\n class=\"ProductDescriptionContainer product-droppdown__section-description\">\n <div class=\"product-tech-info\">\n <div class=\"product-tech-info__line-item\">\n <div class=\"product-tech-info__line-item-left\">\n <b>Immunogen</b>\n </div>\n <div class=\"product-tech-info__line-item-right\">\n Between amino acids 422 and 472\n </div>\n </div>\n <div class=\"product-tech-info__line-item\">\n <div class=\"product-tech-info__line-item-left\">\n <b>Storage</b>\n </div>\n <div class=\"product-tech-info__line-item-right\">\n Stable for 1 year at 4°C from date of receipt\n </div>\n </div>\n <div class=\"product-tech-info__line-item\">\n <div class=\"product-tech-info__line-item-left\">\n <b>Formulation</b>\n </div>\n <div class=\"product-tech-info__line-item-right\">\n Antigen affinity-purified antibody in Tris-citrate/phosphate\n buffer pH 7-8, 0.09% sodium azide\n </div>\n </div>\n </div>\n </div>\n </div>\n </div>\n <div id=\"prodAccordion\">\n <div id=\"ProductDescription\" class=\"Block Panel\">\n <h3 class=\"sub-title1\">Recommended Dilution</h3>\n <div\n class=\"ProductDescriptionContainer product-droppdown__section-description\">\n <div class=\"product-tech-info\">\n <div class=\"product-tech-info__line-item\">\n <div class=\"product-tech-info__line-item-left\">\n <b>CUT&RUN:</b>\n </div>\n <div class=\"product-tech-info__line-item-right\">\n 0.5 µg per reaction\n </div>\n </div>\n <div class=\"product-tech-info__line-item\">\n <div class=\"product-tech-info__line-item-left\">\n <b>Western Blot (WB):</b>\n </div>\n <div class=\"product-tech-info__line-item-right\">\n 1:2,000 - 1:10,000\n </div>\n </div>\n <div class=\"product-tech-info__line-item\">\n <div class=\"product-tech-info__line-item-left\">\n <b>Immunoprecipitation (IP):</b>\n </div>\n <div class=\"product-tech-info__line-item-right\">\n 2 - 10 µg/mg lysate\n </div>\n </div>\n </div>\n </div>\n </div>\n </div>\n <div id=\"prodAccordion\">\n <div id=\"ProductDescription\" class=\"Block Panel\">\n <h3 class=\"sub-title1\">Gene & Protein Information</h3>\n <div\n class=\"ProductDescriptionContainer product-droppdown__section-description\">\n <div class=\"product-tech-info\">\n <div class=\"product-tech-info__line-item\">\n <div class=\"product-tech-info__line-item-left\">\n <b>UniProt ID</b>\n </div>\n <div class=\"product-tech-info__line-item-right\">P55317</div>\n </div>\n <div class=\"product-tech-info__line-item\">\n <div class=\"product-tech-info__line-item-left\">\n <b>Gene Name</b>\n </div>\n <div class=\"product-tech-info__line-item-right\">FOXA1</div>\n </div>\n <div class=\"product-tech-info__line-item\">\n <div class=\"product-tech-info__line-item-left\">\n <b>Protein Name</b>\n </div>\n <div class=\"product-tech-info__line-item-right\">\n Hepatocyte nuclear factor 3-alpha\n </div>\n </div>\n <div class=\"product-tech-info__line-item\">\n <div class=\"product-tech-info__line-item-left\">\n <b>Target Size</b>\n </div>\n <div class=\"product-tech-info__line-item-right\">49 kDa</div>\n </div>\n <div class=\"product-tech-info__line-item\">\n <div class=\"product-tech-info__line-item-left\">\n <b>Alternate Names</b>\n </div>\n <div class=\"product-tech-info__line-item-right\">\n HNF-3-alpha, HNF-3A, Forkhead box protein A1, Transcription\n factor 3A (TCF-3A), HNF3A, TCF3A\n </div>\n </div>\n </div>\n </div>\n </div>\n </div>\n <div id=\"prodAccordion\">\n <div id=\"ProductDescription\" class=\"Block Panel\">\n <h3 class=\"sub-title1\">Documents & Resources</h3>\n <div\n class=\"ProductDescriptionContainer product-droppdown__section-description\">\n <div class=\"product-documents\">\n <a\n href=\"/content/documents/tds/13-2001.pdf\"\n target=\"_new\"\n class=\"product-documents__link\">\n <svg\n version=\"1.1\"\n id=\"Layer_1\"\n 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M44.81,147.9c5.38,0,8.07-2.32,8.07-6.97c0-2.39-0.67-4.16-2-5.31\n c-1.33-1.15-3.36-1.73-6.07-1.73H34.59v14.01H44.81z\" />\n <path\n class=\"product-documents__svg-pdf\"\n d=\"M69.92,125.68h18.91c5.29,0,9.84,0.97,13.66,2.9c3.82,1.93,6.74,4.72,8.76,8.35\n c2.02,3.63,3.04,7.98,3.04,13.04c0,5.06-1,9.42-3,13.08c-2,3.66-4.91,6.45-8.73,8.38c-3.82,1.93-8.4,2.9-13.73,2.9H69.92V125.68z\n M88.07,165.63c10.35,0,15.52-5.22,15.52-15.66c0-10.4-5.17-15.59-15.52-15.59h-7.38v31.26H88.07z\" />\n <path\n class=\"product-documents__svg-pdf\"\n d=\"M122.57,125.68h32.84v8.49h-22.22v11.18h20.84v8.49h-20.84v20.49h-10.63V125.68z\" />\n </g>\n </svg>\n <span class=\"product-documents__info\">Technical Datasheet</span>\n </a>\n </div>\n </div>\n </div>\n </div>\n <div id=\"prodAccordion\">\n <div id=\"ProductDescription\" class=\"Block Panel\">\n <h3 class=\"sub-title1\">Additional Info</h3>\n <div\n class=\"ProductDescriptionContainer product-droppdown__section-description\">\n <p>\n This product is provided for commercial sale under license from\n Bethyl Laboratories, Inc.\n </p>\n </div>\n </div>\n </div>\n </div>\n</div>\n","tags":[],"warranty":"","price":{"without_tax":{"formatted":"$495.00","value":495,"currency":"USD"},"tax_label":"Sales Tax"},"detail_messages":"","availability":"","page_title":"FOXA1/HNF3A Antibody | CUTANA™ CUT&RUN Compatible","cart_url":"https://www.epicypher.com/cart.php","max_purchase_quantity":0,"mpn":null,"upc":null,"options":[],"related_products":[{"id":694,"sku":null,"name":"CUTANA™ pAG-MNase for ChIC/CUT&RUN Workflows","url":"https://www.epicypher.com/products/epigenetics-reagents-and-assays/cutana-pag-mnase-for-chic-cut-and-run-workflows","availability":"","rating":null,"brand":{"name":null},"category":["Epigenetics Kits and Reagents","Epigenetics Kits and Reagents/CUTANA™ ChIC / CUT&RUN Assays"],"summary":"\n \n \n Type: Nuclease\n \n \n Mol Wgt: 43.7 kDa\n \n \n \n ","image":{"data":"https://cdn11.bigcommerce.com/s-y9o92/images/stencil/{:size}/products/694/689/Screen_Shot_2020-02-12_at_11.01.55_AM__17144.1581530752.png?c=2","alt":"CUTANA™ pAG-MNase for ChIC/CUT&RUN Workflows"},"images":[{"data":"https://cdn11.bigcommerce.com/s-y9o92/images/stencil/{:size}/products/694/689/Screen_Shot_2020-02-12_at_11.01.55_AM__17144.1581530752.png?c=2","alt":"CUTANA™ pAG-MNase for ChIC/CUT&RUN Workflows"}],"date_added":"12th Aug 2019","pre_order":false,"show_cart_action":true,"has_options":true,"stock_level":null,"low_stock_level":null,"qty_in_cart":0,"custom_fields":[{"id":1174,"name":"Internal Comment","value":"Excess in bottom of Venom"},{"id":1175,"name":"Internal Comment","value":"Bulk in Psylocke"}],"num_reviews":null,"weight":{"formatted":"0.01 LBS","value":0.01},"demo":false,"price":{"without_tax":{"currency":"USD","formatted":"$315.00","value":315},"price_range":{"min":{"without_tax":{"currency":"USD","formatted":"$315.00","value":315},"tax_label":"Sales 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- Type: Polyclonal
- Host: Rabbit
- Applications: CUT&RUN, WB, IP
- Reactivity: Human, Mouse (Predicted Rat)
- Format: Antigen affinity-purified
- Target Size: 49 kDa
Description
This antibody meets EpiCypher’s “CUTANA Compatible” criteria for performance in Cleavage Under Targets and Release Using Nuclease (CUT&RUN) and/or Cleavage Under Targets and Tagmentation (CUT&Tag) approaches to genomic mapping. Every lot of a CUTANA Compatible antibody is tested in the indicated approach using EpiCypher optimized protocols and determined to yield peaks that show a genomic distribution pattern consistent with reported function(s) of the target protein. FOXA1 antibody produces CUT&RUN peaks above background primarily in intronic, intergenic, and promoter regions (Figure 1) that overlap with known FOXA1 DNA-binding motifs (Figure 2).
Validation Data
CUT&RUN was performed on 500k MCF7 cells with 0.5 µg of either FOXA1/HNF3A or IgG negative control (EpiCypher 13-0042) antibodies using the CUTANA™ ChIC/CUT&RUN Kit v2.0 (EpiCypher 14-1048). Library preparation was performed with 5 ng of DNA (or the total amount recovered if less than 5 ng) using the CUTANA™ CUT&RUN Library Prep Kit (EpiCypher 14-1001/14-1002). Both kit protocols were adapted for high throughput Tecan liquid handling. Libraries were run on an Illumina NextSeq2000 with paired-end sequencing (2x50 bp). Sample sequencing depth was 2.5 million reads (FOXA1) and 2.4 million reads (IgG). Data were aligned to the hg19 genome using Bowtie2. Data were filtered to remove duplicates, multi-aligned reads, and blacklist regions.
Figure 1: FOXA1 peaks in CUT&RUN
CUT&RUN was performed as described above. Peaks were called
with MACS2. (A) Heatmap showing FOXA1 peaks relative
to IgG negative control antibody in aligned rows ranked by
intensity (top to bottom) and colored such that red
indicates high localized enrichment and blue denotes
background signal. (B) The number of peaks that fall
into distinct classes of functionally annotated genomic
regions are shown.
Figure 2: FOXA1 transcription factor binding motif
analysis in CUT&RUN
(A) Homer analysis determined that the FOXA1
consensus motif, represented as a sequence logo position
weight matrix, was enriched under FOXA1 CUT&RUN peaks.
(B) The number of FOXA1 peaks containing consensus
motifs from panel A is represented by a Venn Diagram.
(C) Gene browser shots were generated using the
Integrative Genomics Viewer (IGV, Broad Institute). Three
representative loci show overlap of FOXA1 peaks with
consensus motifs.
Figure 3: Western blot data
Western analysis of FOXA1 in whole cell lysates from MCF-7
and HeLa cells using NETN lysis buffer. The indicated
amounts (µg) of lysate were loaded onto 4-20% SDS-PAGE gel
and analyzed under standard western blot conditions using
FOXA1 antibody (0.1 µg/mL).
Figure 4: Immunoprecipitation of human FOXA1
EpiCypher FOXA1 antibody (6 µg) was used to
immunoprecipitate whole cell lysates isolated from HeLa
cells using NETN lysis buffer (1 mg per IP). A negative
control IgG antibody and positive control antibody to a
different FOXA1 epitope (Bethyl Laboratories) were also used
to demonstrate specificity of the IP. Immunoprecipitates
were loaded onto 4-20% SDS-PAGE gel (20% of IP loaded) and
probed via western blot with EpiCypher FOXA1 antibody (1
µg/mL).
Technical Information
Recommended Dilution
Gene & Protein Information
Documents & Resources
Additional Info
This product is provided for commercial sale under license from Bethyl Laboratories, Inc.