

CHD3 CUTANA™ CUT&RUN Antibody
{"AddThisServiceButtonMeta":"","add_this":[{"annotation":"","service":"facebook"},{"annotation":"","service":"email"},{"annotation":"","service":"print"},{"annotation":"","service":"twitter"},{"annotation":"","service":"linkedin"}],"add_to_wishlist_url":"/wishlist.php?action=add&product_id=835","availability":"","bulk_discount_rates":[],"can_purchase":true,"cart_url":"https://www.epicypher.com/cart.php","category":["Antibodies/CUTANA™ CUT&RUN Antibodies","Antibodies/CUTANA™ CUT&RUN Antibodies/CUT&RUN Antibodies - Chromatin-Associated Proteins","Epigenetics Kits and Reagents/CUTANA™ ChIC / CUT&RUN Assays"],"custom_fields":[{"id":"710","name":"Pack size","value":"100 µL"}],"customizations":[],"description":"<div class=\"product-general-info\">\n <ul class=\"product-general-info__list-left\">\n <li class=\"product-general-info__list-item\">\n <strong>Type: </strong>Polyclonal\n </li>\n <li class=\"product-general-info__list-item\">\n <strong>Host: </strong>Rabbit\n </li>\n <li class=\"product-general-info__list-item\">\n <strong>Target Size: </strong>227 kDa\n </li>\n </ul>\n <ul class=\"product-general-info__list-right\">\n <li class=\"product-general-info__list-item\">\n <strong>Format: </strong>Aff. Pur. IgG\n </li>\n <li class=\"product-general-info__list-item\">\n <strong>Reactivity: </strong>Human, Mouse\n </li>\n <li class=\"product-general-info__list-item\">\n <strong>Applications: </strong>CUT&RUN, IP, IHC\n </li>\n </ul>\n</div>\n<div class=\"service_accordion product-droppdown\">\n <div class=\"container\">\n <div id=\"prodAccordion\">\n <div id=\"ProductDescription\" class=\"Block Panel current\">\n <h3 class=\"sub-title1\">Description</h3>\n <div\n class=\"ProductDescriptionContainer product-droppdown__section-description-specific\"\n >\n <p>\n This antibody meets EpiCypher’s “CUTANA Compatible” criteria for\n performance in Cleavage Under Targets and Release Using Nuclease\n (CUT&RUN) and/or Cleavage Under Targets and Tagmentation\n (CUT&Tag) approaches to genomic mapping. Every lot of a CUTANA\n Compatible antibody is tested in the indicated CUTANA approach using\n <a href=\"https://www.epicypher.com/resources/protocols\"\n >EpiCypher optimized protocols </a\n >and determined to yield peaks that show a genomic distribution\n pattern consistent with reported function(s) of the target protein.\n CHD3 is an understudied member of the nucleosome remodeling and\n deacetylase (NuRD) complex. CHD3 antibody produces CUT&RUN peaks\n above background (<strong>Figure 1</strong>) within gene promoters,\n intergenic regions, and introns (<strong>Figures 1-2</strong>).\n </p>\n </div>\n </div>\n </div>\n <div id=\"prodAccordion\">\n <div id=\"ProductDescription\" class=\"Block Panel current\">\n <h3 class=\"sub-title1\">Validation Data</h3>\n <div\n class=\"ProductDescriptionContainer product-droppdown__section-description-specific\"\n >\n <section class=\"image-picker\">\n <div class=\"image-picker__left\">\n <div\n class=\"image-picker__main-content_active image-picker__main-content\"\n >\n <div class=\"image-picker__header-content\">\n <button class=\"image-picker__left-arrow\">\n <svg\n class=\"image-picker__svg-left\"\n width=\"24\"\n height=\"24\"\n viewBox=\"0 0 24 24\"\n >\n <path\n d=\"M16.67 0l2.83 2.829-9.339 9.175 9.339 9.167-2.83 2.829-12.17-11.996z\"\n />\n </svg>\n </button>\n <a\n href=\"/content/images/products/antibodies/13_2009_CHD3_Heatmap.jpeg\"\n target=\"_new\"\n class=\"image-picker__main-image-link\"\n ><img\n alt=\"13_2009_CHD3_Heatmap\"\n src=\"/content/images/products/antibodies/13_2009_CHD3_Heatmap.jpeg\"\n class=\"image-picker__main-image\"\n />\n <span class=\"image-picker__main-image-caption\"\n >(Click to enlarge)</span\n ></a\n >\n <button class=\"image-picker__right-arrow\">\n <svg\n class=\"image-picker__svg-right\"\n width=\"24\"\n height=\"24\"\n viewBox=\"0 0 24 24\"\n >\n <path\n d=\"M7.33 24l-2.83-2.829 9.339-9.175-9.339-9.167 2.83-2.829 12.17 11.996z\"\n />\n </svg>\n </button>\n </div>\n <p>\n <span class=\"image-picker__span-content\"\n ><strong\n >Figure 1: CHD3 genome-wide enrichment in CUT&RUN </strong\n ><br />\n CUT&RUN was performed using 500,000 K562 cells with 0.5\n µg CHD3 antibody. (<strong>A</strong>) Heatmap of CHD3 peaks\n (MACS2) relative to IgG and H3K4me3 antibodies (0.5 µg;\n EpiCypher\n <a\n href=\"https://www.epicypher.com/products/epigenetics-reagents-and-assays/cutana-chic-cut-run-assays/cutana-rabbit-igg-cut-run-negative-control-antibody\"\n >13-0042</a\n >\n and\n <a\n href=\"https://www.epicypher.com/products/antibodies/snap-chip-certified-antibodies/histone-h3k4me3-antibody-snap-chip-certified-cutana-cut-run-compatible\"\n >13-0041</a\n >, respectively). Aligned rows are ranked by CHD3 peak\n intensity (top to bottom) and colored such that red\n indicates high enrichment and blue denotes background.\n (<strong>B</strong>) The number of CHD3 peaks falling within\n the indicated genomic features is plotted.\n </span>\n </p>\n </div>\n <div class=\"image-picker__main-content\">\n <div class=\"image-picker__header-content\">\n <button class=\"image-picker__left-arrow\">\n <svg\n class=\"image-picker__svg-left\"\n width=\"24\"\n height=\"24\"\n viewBox=\"0 0 24 24\"\n >\n <path\n d=\"M16.67 0l2.83 2.829-9.339 9.175 9.339 9.167-2.83 2.829-12.17-11.996z\"\n />\n </svg>\n </button>\n <a\n href=\"/content/images/products/antibodies/13_2009_CHD3_Browser_Tracks.jpeg\"\n target=\"_new\"\n class=\"image-picker__main-image-link\"\n ><img\n alt=\"13_2009_CHD3_Browser_Tracks\"\n src=\"/content/images/products/antibodies/13_2009_CHD3_Browser_Tracks.jpeg\"\n class=\"image-picker__main-image\"\n />\n <span class=\"image-picker__main-image-caption\"\n >(Click to enlarge)</span\n ></a\n >\n <button class=\"image-picker__right-arrow\">\n <svg\n class=\"image-picker__svg-right\"\n width=\"24\"\n height=\"24\"\n viewBox=\"0 0 24 24\"\n >\n <path\n d=\"M7.33 24l-2.83-2.829 9.339-9.175-9.339-9.167 2.83-2.829 12.17 11.996z\"\n />\n </svg>\n </button>\n </div>\n <p>\n <span class=\"image-picker__span-content\"\n ><strong\n >Figure 2: Representative CHD3 CUT&RUN peaks</strong\n ><br />\n Two gene loci showing CHD3 peaks at a gene promoter (top)\n and an intronic region (bottom) are representative of the\n functional annotation analysis of CHD3 peak localization in\n CUT&RUN (Figure 1). Images were generated using the\n Integrative Genomics Viewer (Broad Institute).\n </span>\n </p>\n </div>\n <div class=\"image-picker__main-content\">\n <div class=\"image-picker__header-content\">\n <button class=\"image-picker__left-arrow\">\n <svg\n class=\"image-picker__svg-left\"\n width=\"24\"\n height=\"24\"\n viewBox=\"0 0 24 24\"\n >\n <path\n d=\"M16.67 0l2.83 2.829-9.339 9.175 9.339 9.167-2.83 2.829-12.17-11.996z\"\n />\n </svg>\n </button>\n <a\n href=\"/content/images/products/antibodies/13_2009_CHD3_IP_v1.jpeg\"\n target=\"_new\"\n class=\"image-picker__main-image-link\"\n ><img\n alt=\"13_2009_CHD3_IP_v1\"\n src=\"/content/images/products/antibodies/13_2009_CHD3_IP_v1.jpeg\"\n class=\"image-picker__main-image\"\n />\n <span class=\"image-picker__main-image-caption\"\n >(Click to enlarge)</span\n ></a\n >\n <button class=\"image-picker__right-arrow\">\n <svg\n class=\"image-picker__svg-right\"\n width=\"24\"\n height=\"24\"\n viewBox=\"0 0 24 24\"\n >\n <path\n d=\"M7.33 24l-2.83-2.829 9.339-9.175-9.339-9.167 2.83-2.829 12.17 11.996z\"\n />\n </svg>\n </button>\n </div>\n <p>\n <span class=\"image-picker__span-content\"\n ><strong>Figure 3: Immunoprecipitation of human CHD3</strong\n ><br />\n EpiCypher CHD3 antibody (3 µg) was used to immunoprecipitate\n whole cell lysates isolated from HeLa cells using NETN lysis\n buffer (1.0 mg per IP). A negative control IgG antibody and\n positive control antibody to a different CHD3 epitope\n (Bethyl Laboratories) were also used to demonstrate\n specificity of the IP. Immunoprecipitates were loaded onto\n 4-8% SDSPAGE gel (20% of IP loaded) and probed via western\n blot with Bethyl A301-219A antibody (1.0 µg/mL).\n </span>\n </p>\n </div>\n <div class=\"image-picker__main-content\">\n <div class=\"image-picker__header-content\">\n <button class=\"image-picker__left-arrow\">\n <svg\n class=\"image-picker__svg-left\"\n width=\"24\"\n height=\"24\"\n viewBox=\"0 0 24 24\"\n >\n <path\n d=\"M16.67 0l2.83 2.829-9.339 9.175 9.339 9.167-2.83 2.829-12.17-11.996z\"\n />\n </svg>\n </button>\n <a\n href=\"/content/images/products/antibodies/13_2009_CHD3_IHC.jpeg\"\n target=\"_new\"\n class=\"image-picker__main-image-link\"\n ><img\n alt=\"13_2009_CHD3_IHC\"\n src=\"/content/images/products/antibodies/13_2009_CHD3_IHC.jpeg\"\n class=\"image-picker__main-image\"\n />\n <span class=\"image-picker__main-image-caption\"\n >(Click to enlarge)</span\n ></a\n >\n <button class=\"image-picker__right-arrow\">\n <svg\n class=\"image-picker__svg-right\"\n width=\"24\"\n height=\"24\"\n viewBox=\"0 0 24 24\"\n >\n <path\n d=\"M7.33 24l-2.83-2.829 9.339-9.175-9.339-9.167 2.83-2.829 12.17 11.996z\"\n />\n </svg>\n </button>\n </div>\n <p>\n <span class=\"image-picker__span-content\"\n ><strong\n >Figure 4: Immunohistochemistry detection of human and\n mouse CHD3</strong\n ><br />\n FFPE section of human Ewing sarcoma (left) and mouse renal\n cell carcinoma (right) examined using CHD3 antibody (1:1,000\n dilution, 1 µg/mL).\n </span>\n </p>\n </div>\n </div>\n <aside class=\"image-picker__right\">\n <div class=\"image-picker__gallery\">\n <img\n alt=\"13_2009_CHD3_Heatmap\"\n src=\"/content/images/products/antibodies/13_2009_CHD3_Heatmap.jpeg\"\n width=\"200\"\n class=\"image-picker__side-image image-picker__side-image_active\"\n role=\"button\"\n />\n <img\n alt=\"13_2009_CHD3_Browser_Tracks\"\n src=\"/content/images/products/antibodies/13_2009_CHD3_Browser_Tracks.jpeg\"\n class=\"image-picker__side-image\"\n role=\"button\"\n />\n <img\n alt=\"13_2009_CHD3_IP_v1\"\n src=\"/content/images/products/antibodies/13_2009_CHD3_IP_v1.jpeg\"\n class=\"image-picker__side-image\"\n role=\"button\"\n />\n <img\n alt=\"13_2009_CHD3_IHC\"\n src=\"/content/images/products/antibodies/13_2009_CHD3_IHC.jpeg\"\n class=\"image-picker__side-image\"\n role=\"button\"\n />\n </div>\n </aside>\n </section>\n </div>\n </div>\n </div>\n <div id=\"prodAccordion\">\n <div id=\"ProductDescription\" class=\"Block Panel\">\n <h3 class=\"sub-title1\">Technical Information</h3>\n <div\n class=\"ProductDescriptionContainer product-droppdown__section-description\"\n >\n <div class=\"product-tech-info\">\n <div class=\"product-tech-info__line-item\">\n <div class=\"product-tech-info__line-item-left\">\n <b>Immunogen</b>\n </div>\n\n <div class=\"product-tech-info__line-item-right\">\n A synthetic peptide corresponding to human CHD3 amino acids 1950\n to 2000.\n </div>\n </div>\n <div class=\"product-tech-info__line-item\">\n <div class=\"product-tech-info__line-item-left\">\n <b>Formulation</b>\n </div>\n <div class=\"product-tech-info__line-item-right\">\n Antigen affinity-purified antibody (1.0 mg/mL) in\n Tris-citrate/phosphate buffer pH 7 to 8, 0.09% sodium azide.\n </div>\n </div>\n <div class=\"product-tech-info__line-item\">\n <div class=\"product-tech-info__line-item-left\">\n <b>Storage and Stability</b>\n </div>\n <div class=\"product-tech-info__line-item-right\">\n Stable for 1 year at 4°C from date of receipt.\n </div>\n </div>\n </div>\n </div>\n </div>\n </div>\n <div id=\"prodAccordion\">\n <div id=\"ProductDescription\" class=\"Block Panel\">\n <h3 class=\"sub-title1\">Application Notes</h3>\n <div\n class=\"ProductDescriptionContainer product-droppdown__section-description\"\n >\n <p><strong>Recommended Dilutions:</strong></p>\n <p><strong>CUT&RUN:</strong> 0.5 µg</p>\n <p><strong>IHC:</strong> 1:500 - 1:2,000*</p>\n <p><strong>IP:</strong> 2 - 5 µg/mg lysate</p>\n <p>\n <em\n >*Epitope retrieval with citrate buffer pH 6.0 recommended for\n FFPE tissue</em\n >\n </p>\n </div>\n </div>\n </div>\n <div id=\"prodAccordion\">\n <div id=\"ProductDescription\" class=\"Block Panel\">\n <h3 class=\"sub-title1\">Documents & Resources</h3>\n <div\n class=\"ProductDescriptionContainer product-droppdown__section-description\"\n >\n <div class=\"product-documents\">\n <a\n href=\"/content/documents/tds/13-2009.pdf\"\n target=\"_new\"\n class=\"product-documents__link\"\n >\n <svg\n version=\"1.1\"\n id=\"Layer_1\"\n xmlns=\"http://www.w3.org/2000/svg\"\n xmlns:xlink=\"http://www.w3.org/1999/xlink\"\n x=\"0px\"\n y=\"0px\"\n viewBox=\"0 0 228 240\"\n style=\"enable-background: new 0 0 228 240\"\n xml:space=\"preserve\"\n class=\"product-documents__icon\"\n >\n <g>\n <path\n class=\"product-documents__svg-pdf\"\n d=\"M191.92,68.77l-47.69-47.69c-1.33-1.33-3.12-2.08-5.01-2.08H45.09C41.17,19,38,22.17,38,26.09v184.36\n c0,3.92,3.17,7.09,7.09,7.09h141.82c3.92,0,7.09-3.17,7.09-7.09V73.8C194,71.92,193.25,70.1,191.92,68.77z M177.65,77.06h-41.7\n v-41.7L177.65,77.06z M178.05,201.59H53.95V34.95h66.92v47.86c0,5.14,4.17,9.31,9.31,9.31h47.86V201.59z\"\n />\n </g>\n <rect\n x=\"20\"\n y=\"112\"\n class=\"product-documents__svg-background\"\n width=\"146\"\n height=\"76\"\n />\n <g>\n <path\n class=\"product-documents__svg-pdf\"\n d=\"M23.83,125.68h22.36c5.29,0,9.41,1.33,12.35,4c2.94,2.67,4.42,6.39,4.42,11.18c0,4.78-1.47,8.51-4.42,11.18\n c-2.94,2.67-7.06,4-12.35,4H34.59v18.29H23.83V125.68z M44.81,147.9c5.38,0,8.07-2.32,8.07-6.97c0-2.39-0.67-4.16-2-5.31\n c-1.33-1.15-3.36-1.73-6.07-1.73H34.59v14.01H44.81z\"\n />\n <path\n class=\"product-documents__svg-pdf\"\n d=\"M69.92,125.68h18.91c5.29,0,9.84,0.97,13.66,2.9c3.82,1.93,6.74,4.72,8.76,8.35\n c2.02,3.63,3.04,7.98,3.04,13.04c0,5.06-1,9.42-3,13.08c-2,3.66-4.91,6.45-8.73,8.38c-3.82,1.93-8.4,2.9-13.73,2.9H69.92V125.68z\n M88.07,165.63c10.35,0,15.52-5.22,15.52-15.66c0-10.4-5.17-15.59-15.52-15.59h-7.38v31.26H88.07z\"\n />\n <path\n class=\"product-documents__svg-pdf\"\n d=\"M122.57,125.68h32.84v8.49h-22.22v11.18h20.84v8.49h-20.84v20.49h-10.63V125.68z\"\n />\n </g>\n </svg>\n <span class=\"product-documents__info\">Technical Datasheet</span>\n </a>\n </div>\n </div>\n </div>\n </div>\n <div id=\"prodAccordion\">\n <div id=\"ProductDescription\" class=\"Block Panel\">\n <h3 class=\"sub-title1\">Additional Info</h3>\n <div\n class=\"ProductDescriptionContainer product-droppdown__section-description\"\n >\n <p>\n This product is provided for commercial sale under license from\n Bethyl Laboratories, Inc.\n </p>\n\n <strong>Applications Key:</strong> <br /><br />\n\n ChIP: Chromatin immunoprecipitation<br />\n CUT&RUN: Cleavage Under Targets and Release Using Nuclease<br />\n CUT&Tag: Cleavage Under Targets and Tagmentation<br />\n E: ELISA<br />\n FACS: Flow cytometry<br />\n ICC: Immunocytochemistry<br />\n IF: Immunofluorescence<br />\n IHC: Immunohistochemistry<br />\n IP: Immunoprecipitation<br />\n L: Luminex<br />\n WB: Western Blot<br /><br />\n\n <strong>Reactivity Key:</strong> <br /><br />\n\n B: Bovine<br />\n Ce: <em>C. elegans</em><br />\n Ch: Chicken<br />\n Dm: <em>Drosophila</em><br />\n Eu: Eukaryote<br />\n H: Human<br />\n M: Mouse<br />\n Ma: Mammal<br />\n R: Rat<br />\n Sc: <em>S. cerevisiae</em><br />\n Sp: <em>S. pombe</em><br />\n WR: Wide Range (predicted)<br />\n X: Xenopus<br />\n Z: Zebrafish<br />\n </div>\n </div>\n </div>\n </div>\n</div>\n","detail_messages":"","gift_wrapping_available":false,"gtin":null,"id":"835","images":[{"alt":"CHD3 CUTANA™ CUT&RUN Antibody","data":"https://cdn11.bigcommerce.com/s-y9o92/images/stencil/{:size}/products/835/1006/chd3-cutana-cutandrun-antibody__66975.1645734525__91145.1648666822.jpg?c=2"},{"alt":"CHD3 CUTANA CUTandRUN Antibody","data":"https://cdn11.bigcommerce.com/s-y9o92/images/stencil/{:size}/products/835/934/chd3-cutana-cutandrun-antibody__66975.1648667309.jpg?c=2"}],"main_image":{"alt":"CHD3 CUTANA™ CUT&RUN Antibody","data":"https://cdn11.bigcommerce.com/s-y9o92/images/stencil/{:size}/products/835/1006/chd3-cutana-cutandrun-antibody__66975.1645734525__91145.1648666822.jpg?c=2"},"max_purchase_quantity":0,"meta_description":"Polyclonal anti-CHD3 antibody validated for CUT&RUN, IP, IHC. 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- Type: Polyclonal
- Host: Rabbit
- Target Size: 227 kDa
- Format: Aff. Pur. IgG
- Reactivity: Human, Mouse
- Applications: CUT&RUN, IP, IHC
Description
This antibody meets EpiCypher’s “CUTANA Compatible” criteria for performance in Cleavage Under Targets and Release Using Nuclease (CUT&RUN) and/or Cleavage Under Targets and Tagmentation (CUT&Tag) approaches to genomic mapping. Every lot of a CUTANA Compatible antibody is tested in the indicated CUTANA approach using EpiCypher optimized protocols and determined to yield peaks that show a genomic distribution pattern consistent with reported function(s) of the target protein. CHD3 is an understudied member of the nucleosome remodeling and deacetylase (NuRD) complex. CHD3 antibody produces CUT&RUN peaks above background (Figure 1) within gene promoters, intergenic regions, and introns (Figures 1-2).
Validation Data
Figure 1: CHD3 genome-wide enrichment in CUT&RUN
CUT&RUN was performed using 500,000 K562 cells with 0.5
µg CHD3 antibody. (A) Heatmap of CHD3 peaks
(MACS2) relative to IgG and H3K4me3 antibodies (0.5 µg;
EpiCypher
13-0042
and
13-0041, respectively). Aligned rows are ranked by CHD3 peak
intensity (top to bottom) and colored such that red
indicates high enrichment and blue denotes background.
(B) The number of CHD3 peaks falling within
the indicated genomic features is plotted.
Figure 2: Representative CHD3 CUT&RUN peaks
Two gene loci showing CHD3 peaks at a gene promoter (top)
and an intronic region (bottom) are representative of the
functional annotation analysis of CHD3 peak localization in
CUT&RUN (Figure 1). Images were generated using the
Integrative Genomics Viewer (Broad Institute).
Figure 3: Immunoprecipitation of human CHD3
EpiCypher CHD3 antibody (3 µg) was used to immunoprecipitate
whole cell lysates isolated from HeLa cells using NETN lysis
buffer (1.0 mg per IP). A negative control IgG antibody and
positive control antibody to a different CHD3 epitope
(Bethyl Laboratories) were also used to demonstrate
specificity of the IP. Immunoprecipitates were loaded onto
4-8% SDSPAGE gel (20% of IP loaded) and probed via western
blot with Bethyl A301-219A antibody (1.0 µg/mL).
Figure 4: Immunohistochemistry detection of human and
mouse CHD3
FFPE section of human Ewing sarcoma (left) and mouse renal
cell carcinoma (right) examined using CHD3 antibody (1:1,000
dilution, 1 µg/mL).
Technical Information
Application Notes
Recommended Dilutions:
CUT&RUN: 0.5 µg
IHC: 1:500 - 1:2,000*
IP: 2 - 5 µg/mg lysate
*Epitope retrieval with citrate buffer pH 6.0 recommended for FFPE tissue
Documents & Resources
Additional Info
This product is provided for commercial sale under license from Bethyl Laboratories, Inc.
Applications Key:ChIP: Chromatin immunoprecipitation
CUT&RUN: Cleavage Under Targets and Release Using Nuclease
CUT&Tag: Cleavage Under Targets and Tagmentation
E: ELISA
FACS: Flow cytometry
ICC: Immunocytochemistry
IF: Immunofluorescence
IHC: Immunohistochemistry
IP: Immunoprecipitation
L: Luminex
WB: Western Blot
Reactivity Key:
B: Bovine
Ce: C. elegans
Ch: Chicken
Dm: Drosophila
Eu: Eukaryote
H: Human
M: Mouse
Ma: Mammal
R: Rat
Sc: S. cerevisiae
Sp: S. pombe
WR: Wide Range (predicted)
X: Xenopus
Z: Zebrafish