CHD1

CHD1 CUTANA™ CUT&RUN Antibody

$445.00
SKU: 13-2008
Pack Size: 100 µL
  • Type:  Polyclonal
  • Host:  Rabbit
  • Target Size:  197 kDa
  • Format:  Affinity Purified IgG
  • Reactivity:  Human
  • Applications: CUT&RUN, IP

Description

This antibody meets EpiCypher’s “CUTANA Compatible” criteria for performance in Cleavage Under Targets and Release Using Nuclease (CUT&RUN) and/or Cleavage Under Targets and Tagmentation (CUT&Tag) approaches to genomic mapping. Every lot of a CUTANA Compatible antibody is tested in the indicated CUTANA approach using EpiCypher optimized protocols and determined to yield peaks that show a genomic distribution pattern consistent with reported function(s) of the target protein. CHD1 antibody produces CUT&RUN peaks above background (Figure 1) that overlap with H3K4me3 (Figures 1-2), consistent with CHD1 tandem chromodomains that mediate H3K4me3 binding and SWI/SNF complex nucleosome remodeling [1].

Validation Data

Figure 1: CHD1 enrichment at annotated transcription start sites (TSSs) in CUT&RUN
CUT&RUN was performed using 500,000 K562 cells with CHD1 (0.1 µg) and control antibodies (0.5 µg; IgG negative control, EpiCypher 13-0042; H3K4me3 positive control, EpiCypher 13-0041). Sequencing reads were aligned to annotated TSSs (+/- 2 kbp) of 18,793 genes. High, medium, and low signal is ranked by intensity (top to bottom) and reflected by red, yellow, and blue colors, respectively. Rows aligned relative to CHD1 antibody.

Figure 2: CHD1 CUT&RUN peaks and functional overlap
Two representative gene loci from the CUT&RUN data in Figure 1 are shown. CHD1 enrichment overlaps with H3K4me3 CUT&RUN peaks (EpiCypher 13-0041), consistent with the CHD1 double chromodomain recognition of this PTM (1). Images were generated using the Integrative Genomics Viewer (Broad Institute).

Figure 3: Immunoprecipitation of human CHD1
EpiCypher CHD1 antibody (3 µg) was used to immunoprecipitate whole cell lysates isolated from HeLa cells using NETN lysis buffer (1.0 mg per IP). A negative control IgG antibody and positive control antibody to a different CHD1 epitope (Bethyl Laboratories) were also used to demonstrate specificity of the IP. Immunoprecipitates were loaded onto 4-8% SDSPAGE gel (20% of IP loaded) and probed via western blot with Bethyl A301-218A antibody (1.0 µg/mL).

Technical Information

Immunogen
A synthetic peptide corresponding to human CHD1 amino acids 1500 to 1550.
Formulation
Antigen affinity-purified antibody (1.0 mg/mL) in Triscitrate/phosphate buffer pH 7 to 8, 0.09% sodium azide.
Storage and Stability
Stable for 1 year at 4°C from date of receipt.

Application Notes

Recommended Dilutions:

CUT&RUN: 0.5 µg

Immunoprecipitation (IP): 2 - 5 µg/mg lysate

References

Background References:
[1] Flanagan et al. Nature (2005). PMID: 16372014

Documents & Resources

Additional Info

This product is provided for commercial sale under license from Bethyl Laboratories, Inc.

Applications Key:

ChIP: Chromatin immunoprecipitation
CUT&RUN: Cleavage Under Targets and Release Using Nuclease
CUT&Tag: Cleavage Under Targets and Tagmentation
E: ELISA
FACS: Flow cytometry
ICC: Immunocytochemistry
IF: Immunofluorescence
IHC: Immunohistochemistry
IP: Immunoprecipitation
L: Luminex
WB: Western Blot

Reactivity Key:

B: Bovine
Ce: C. elegans
Ch: Chicken
Dm: Drosophila
Eu: Eukaryote
H: Human
M: Mouse
Ma: Mammal
R: Rat
Sc: S. cerevisiae
Sp: S. pombe
WR: Wide Range (predicted)
X: Xenopus
Z: Zebrafish
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