

BRD4 CUTANA™ CUT&RUN Antibody
{"AddThisServiceButtonMeta":"","add_this":[{"annotation":"","service":"facebook"},{"annotation":"","service":"email"},{"annotation":"","service":"print"},{"annotation":"","service":"twitter"},{"annotation":"","service":"linkedin"}],"add_to_wishlist_url":"/wishlist.php?action=add&product_id=798","availability":"","bulk_discount_rates":[],"can_purchase":true,"cart_url":"https://www.epicypher.com/cart.php","category":["Antibodies","Antibodies/CUTANA™ CUT&RUN Antibodies","Antibodies/CUTANA™ CUT&RUN Antibodies/CUT&RUN Antibodies - Chromatin-Associated Proteins","Epigenetics Kits and Reagents/CUTANA™ ChIC / CUT&RUN Assays"],"custom_fields":[{"id":"655","name":"Pack size","value":"50 µL"}],"customizations":[],"description":"<div class=\"product-general-info\">\n <ul class=\"product-general-info__list-left\">\n <li class=\"product-general-info__list-item\">\n <strong>Type: </strong>Polyclonal\n </li>\n <li class=\"product-general-info__list-item\">\n <strong>Host: </strong>Rabbit\n </li>\n <li class=\"product-general-info__list-item\">\n <strong>Target Size: </strong>152 kDa\n </li>\n </ul>\n <ul class=\"product-general-info__list-right\">\n <li class=\"product-general-info__list-item\">\n <strong>Format: </strong>Affinity Purified IgG\n </li>\n <li class=\"product-general-info__list-item\">\n <strong>Reactivity: </strong>Human, Mouse\n </li>\n <li class=\"product-general-info__list-item\">\n <strong>Applications: </strong>CUT&RUN, WB, IP, IHC\n </li>\n </ul>\n</div>\n<div class=\"service_accordion product-droppdown\">\n <div class=\"container\">\n <div id=\"prodAccordion\">\n <div id=\"ProductDescription\" class=\"Block Panel current\">\n <h3 class=\"sub-title1\">Description</h3>\n <div\n class=\"\n ProductDescriptionContainer\n product-droppdown__section-description-specific\n \"\n >\n <p>\n This antibody meets EpiCypher’s “CUTANA Compatible” criteria for\n performance in Cleavage Under Targets and Release Using Nuclease\n (CUT&RUN) and/or Cleavage Under Targets and Tagmentation\n (CUT&Tag) approaches to genomic mapping. Every lot of a CUTANA\n Compatible antibody is tested in the indicated CUTANA approach using\n <a href=\"/resources/protocols\">EpiCypher optimized protocols </a>and\n determined to yield peaks that show a genomic distribution pattern\n consistent with reported function(s) of the target protein. BRD4\n antibody produces CUT&RUN peaks primarily flanking transcription\n start sites (TSSs, <strong>Figure 1</strong>). BRD4 peaks show a\n large degree of overlap with BRG1/SMARCA4 peaks\n <strong>(Figure 2)</strong>, as has been reported in the literature\n (Conrad et al., 2017).\n </p>\n </div>\n </div>\n </div>\n <div id=\"prodAccordion\">\n <div id=\"ProductDescription\" class=\"Block Panel current\">\n <h3 class=\"sub-title1\">Validation Data</h3>\n <div\n class=\"\n ProductDescriptionContainer\n product-droppdown__section-description-specific\n \"\n >\n <section class=\"image-picker\">\n <div class=\"image-picker__left\">\n <div\n class=\"\n image-picker__main-content_active image-picker__main-content\n \"\n >\n <div class=\"image-picker__header-content\">\n <button class=\"image-picker__left-arrow\">\n <svg\n class=\"image-picker__svg-left\"\n width=\"24\"\n height=\"24\"\n viewBox=\"0 0 24 24\"\n >\n <path\n d=\"M16.67 0l2.83 2.829-9.339 9.175 9.339 9.167-2.83 2.829-12.17-11.996z\"\n />\n </svg>\n </button>\n <a\n href=\"/content/images/products/antibodies/13_2003_Heatmap.jpg\"\n target=\"_new\"\n class=\"image-picker__main-image-link\"\n ><img\n alt=\"13_2003_Heatmap\"\n src=\"/content/images/products/antibodies/13_2003_Heatmap.jpg\"\n class=\"image-picker__main-image\"\n />\n <span class=\"image-picker__main-image-caption\"\n >(Click to enlarge)</span\n ></a\n >\n <button class=\"image-picker__right-arrow\">\n <svg\n class=\"image-picker__svg-right\"\n width=\"24\"\n height=\"24\"\n viewBox=\"0 0 24 24\"\n >\n <path\n d=\"M7.33 24l-2.83-2.829 9.339-9.175-9.339-9.167 2.83-2.829 12.17 11.996z\"\n />\n </svg>\n </button>\n </div>\n <p>\n <span class=\"image-picker__span-content\"\n ><strong\n >Figure 1: BRD4 enrichment at annotated TSSs in\n CUT&RUN </strong\n ><br />\n CUT&RUN was performed using 500,000 K562 cells with BRD4\n antibody as well as control antibodies (IgG negative\n control, EpiCypher\n <a\n href=\"/products/epigenetics-reagents-and-assays/cutana-chic-cut-run-assays/cutana-rabbit-igg-cut-run-negative-control-antibody\"\n >13-0042</a\n >; H3K4me3 positive control, EpiCypher\n <a\n href=\"/products/antibodies/snap-chip-certified-antibodies/histone-h3k4me3-antibody-snap-chip-certified-cutana-cut-run-compatible\"\n >13-0041</a\n >). Sequencing reads were aligned to annotated TSSs (+/- 2\n kbp) of 18,793 genes. High, medium, and low signal is ranked\n by intensity (top to bottom) and reflected by red, yellow,\n and blue colors, respectively. All rows aligned relative to\n H3K4me3 antibody.\n </span>\n </p>\n </div>\n <div class=\"image-picker__main-content\">\n <div class=\"image-picker__header-content\">\n <button class=\"image-picker__left-arrow\">\n <svg\n class=\"image-picker__svg-left\"\n width=\"24\"\n height=\"24\"\n viewBox=\"0 0 24 24\"\n >\n <path\n d=\"M16.67 0l2.83 2.829-9.339 9.175 9.339 9.167-2.83 2.829-12.17-11.996z\"\n />\n </svg>\n </button>\n <a\n href=\"/content/images/products/antibodies/13_2003_Tracks.jpg\"\n target=\"_new\"\n class=\"image-picker__main-image-link\"\n ><img\n alt=\"13_2003_tracks\"\n src=\"/content/images/products/antibodies/13_2003_Tracks.jpg\"\n class=\"image-picker__main-image\"\n />\n <span class=\"image-picker__main-image-caption\"\n >(Click to enlarge)</span\n ></a\n >\n <button class=\"image-picker__right-arrow\">\n <svg\n class=\"image-picker__svg-right\"\n width=\"24\"\n height=\"24\"\n viewBox=\"0 0 24 24\"\n >\n <path\n d=\"M7.33 24l-2.83-2.829 9.339-9.175-9.339-9.167 2.83-2.829 12.17 11.996z\"\n />\n </svg>\n </button>\n </div>\n <p>\n <span class=\"image-picker__span-content\"\n ><strong\n >Figure 2: BRD4 CUT&RUN peak enrichment and functional\n overlap</strong\n ><br />\n The CUT&RUN data from Figure 1 was subjected to peak calling\n using MACS2. BRD4 peaks overlapped with BRG1/ SMARCA4\n antibody CUT&RUN peaks (EpiCypher\n <a\n href=\"/products/antibodies/brg1-smarca4-cutana-cut-run-antibody\"\n >13-2002</a\n >, top), as has been demonstrated in the literature (Conrad\n et al., 2017). Three representative loci show BRD4 peaks in\n relation to control and BRG1 antibodies (bottom, Integrative\n Genomics Viewer).\n </span>\n </p>\n </div>\n <div class=\"image-picker__main-content\">\n <div class=\"image-picker__header-content\">\n <button class=\"image-picker__left-arrow\">\n <svg\n class=\"image-picker__svg-left\"\n width=\"24\"\n height=\"24\"\n viewBox=\"0 0 24 24\"\n >\n <path\n d=\"M16.67 0l2.83 2.829-9.339 9.175 9.339 9.167-2.83 2.829-12.17-11.996z\"\n />\n </svg>\n </button>\n <a\n href=\"/content/images/products/antibodies/13_2003_WB_H.jpg\"\n target=\"_new\"\n class=\"image-picker__main-image-link\"\n ><img\n alt=\"13_2003_WB_H\"\n src=\"/content/images/products/antibodies/13_2003_WB_H.jpg\"\n class=\"image-picker__main-image\"\n />\n <span class=\"image-picker__main-image-caption\"\n >(Click to enlarge)</span\n ></a\n >\n <button class=\"image-picker__right-arrow\">\n <svg\n class=\"image-picker__svg-right\"\n width=\"24\"\n height=\"24\"\n viewBox=\"0 0 24 24\"\n >\n <path\n d=\"M7.33 24l-2.83-2.829 9.339-9.175-9.339-9.167 2.83-2.829 12.17 11.996z\"\n />\n </svg>\n </button>\n </div>\n <p>\n <span class=\"image-picker__span-content\"\n ><strong\n >Figure 3: Western blot detection of human BRD4</strong\n ><br />\n Whole cell lysates were isolated from HeLa, HEK293T\n (“293T”), Jurkat, MB231, Hep-G2, A-549, SW620, SK-MEL-28\n (“SK”), and Ramos cells using NETN lysis buffer. Lysates (15\n µg) were loaded onto 4-8% SDS-PAGE gel and analyzed under\n standard western blot conditions using BRD4 antibody\n (1:1,000 dilution).\n </span>\n </p>\n </div>\n <div class=\"image-picker__main-content\">\n <div class=\"image-picker__header-content\">\n <button class=\"image-picker__left-arrow\">\n <svg\n class=\"image-picker__svg-left\"\n width=\"24\"\n height=\"24\"\n viewBox=\"0 0 24 24\"\n >\n <path\n d=\"M16.67 0l2.83 2.829-9.339 9.175 9.339 9.167-2.83 2.829-12.17-11.996z\"\n />\n </svg>\n </button>\n <a\n href=\"/content/images/products/antibodies/13_2003_WB_M.jpg\"\n target=\"_new\"\n class=\"image-picker__main-image-link\"\n ><img\n alt=\"13_2003_WB_M\"\n src=\"/content/images/products/antibodies/13_2003_WB_M.jpg\"\n class=\"image-picker__main-image\"\n />\n <span class=\"image-picker__main-image-caption\"\n >(Click to enlarge)</span\n ></a\n >\n <button class=\"image-picker__right-arrow\">\n <svg\n class=\"image-picker__svg-right\"\n width=\"24\"\n height=\"24\"\n viewBox=\"0 0 24 24\"\n >\n <path\n d=\"M7.33 24l-2.83-2.829 9.339-9.175-9.339-9.167 2.83-2.829 12.17 11.996z\"\n />\n </svg>\n </button>\n </div>\n <p>\n <span class=\"image-picker__span-content\"\n ><strong\n >Figure 4: Western blot detection of mouse BRD4</strong\n ><br />\n Whole cell lysates were isolated from NIH 3T3 (“3T3”) cells\n using NETN lysis buffer. Lysates (15 µg) were loaded onto\n 4-8% SDS-PAGE gel and analyzed under standard western blot\n conditions using BRD4 antibody (0.1 µg/mL).\n </span>\n </p>\n </div>\n <div class=\"image-picker__main-content\">\n <div class=\"image-picker__header-content\">\n <button class=\"image-picker__left-arrow\">\n <svg\n class=\"image-picker__svg-left\"\n width=\"24\"\n height=\"24\"\n viewBox=\"0 0 24 24\"\n >\n <path\n d=\"M16.67 0l2.83 2.829-9.339 9.175 9.339 9.167-2.83 2.829-12.17-11.996z\"\n />\n </svg>\n </button>\n <a\n href=\"/content/images/products/antibodies/13_2003_IP.jpg\"\n target=\"_new\"\n class=\"image-picker__main-image-link\"\n ><img\n alt=\"13_2003_IP\"\n src=\"/content/images/products/antibodies/13_2003_IP.jpg\"\n class=\"image-picker__main-image\"\n />\n <span class=\"image-picker__main-image-caption\"\n >(Click to enlarge)</span\n ></a\n >\n <button class=\"image-picker__right-arrow\">\n <svg\n class=\"image-picker__svg-right\"\n width=\"24\"\n height=\"24\"\n viewBox=\"0 0 24 24\"\n >\n <path\n d=\"M7.33 24l-2.83-2.829 9.339-9.175-9.339-9.167 2.83-2.829 12.17 11.996z\"\n />\n </svg>\n </button>\n </div>\n <p>\n <span class=\"image-picker__span-content\"\n ><strong>Figure 5: Immunoprecipitation of human BRD4</strong\n ><br />\n EpiCypher BRD4 antibody (3 µg) was used to immunoprecipitate\n whole cell lysates isolated from HeLa cells using NETN lysis\n buffer (1 mg per IP). A negative control IgG antibody and\n positive control antibodies to a different BRD4 epitopes\n (Bethyl Laboratories) were also used to demonstrate\n specificity of the IP. Immunoprecipitates were loaded onto\n 4-8% SDS-PAGE gel (20% of IP loaded) and probed via western\n blot with a different BRD4 antibody (Bethyl Laboratories,\n Catalog No. A700-004, 1:1,000).\n </span>\n </p>\n </div>\n <div class=\"image-picker__main-content\">\n <div class=\"image-picker__header-content\">\n <button class=\"image-picker__left-arrow\">\n <svg\n class=\"image-picker__svg-left\"\n width=\"24\"\n height=\"24\"\n viewBox=\"0 0 24 24\"\n >\n <path\n d=\"M16.67 0l2.83 2.829-9.339 9.175 9.339 9.167-2.83 2.829-12.17-11.996z\"\n />\n </svg>\n </button>\n <a\n href=\"/content/images/products/antibodies/13_2003_IHC.jpg\"\n target=\"_new\"\n class=\"image-picker__main-image-link\"\n ><img\n alt=\"13_2003_IHC\"\n src=\"/content/images/products/antibodies/13_2003_IHC.jpg\"\n class=\"image-picker__main-image\"\n />\n <span class=\"image-picker__main-image-caption\"\n >(Click to enlarge)</span\n ></a\n >\n <button class=\"image-picker__right-arrow\">\n <svg\n class=\"image-picker__svg-right\"\n width=\"24\"\n height=\"24\"\n viewBox=\"0 0 24 24\"\n >\n <path\n d=\"M7.33 24l-2.83-2.829 9.339-9.175-9.339-9.167 2.83-2.829 12.17 11.996z\"\n />\n </svg>\n </button>\n </div>\n <p>\n <span class=\"image-picker__span-content\"\n ><strong\n >Figure 6: Immunohistochemistry detection of human and\n mouse BRD4</strong\n ><br />\n <strong>(A)</strong> FFPE section of human ovarian carcinoma\n examined using BRD4 antibody (1:5,000 dilution, 0.2 µg/mL).\n <strong>(B)</strong> FFPE section of mouse CT26 colon\n carcinoma examined using BRD4 antibody (1:1,000 dilution, 1\n µg/mL).\n </span>\n </p>\n </div>\n </div>\n <aside class=\"image-picker__right\">\n <div class=\"image-picker__gallery\">\n <img\n alt=\"13_2003_Heatmap\"\n src=\"/content/images/products/antibodies/13_2003_Heatmap.jpg\"\n width=\"200\"\n class=\"\n image-picker__side-image image-picker__side-image_active\n \"\n role=\"button\"\n />\n <img\n alt=\"13_2003_tracks\"\n src=\"/content/images/products/antibodies/13_2003_Tracks.jpg\"\n class=\"image-picker__side-image\"\n role=\"button\"\n />\n <img\n alt=\"13_2003_WB_H\"\n src=\"/content/images/products/antibodies/13_2003_WB_H.jpg\"\n class=\"image-picker__side-image\"\n role=\"button\"\n />\n <img\n alt=\"13_2003_WB_M\"\n src=\"/content/images/products/antibodies/13_2003_WB_M.jpg\"\n class=\"image-picker__side-image\"\n role=\"button\"\n />\n <img\n alt=\"13_2003_IP\"\n src=\"/content/images/products/antibodies/13_2003_IP.jpg\"\n class=\"image-picker__side-image\"\n role=\"button\"\n />\n <img\n alt=\"13_2003_IHC\"\n src=\"/content/images/products/antibodies/13_2003_IHC.jpg\"\n class=\"image-picker__side-image\"\n role=\"button\"\n />\n </div>\n </aside>\n </section>\n </div>\n </div>\n </div>\n <div id=\"prodAccordion\">\n <div id=\"ProductDescription\" class=\"Block Panel\">\n <h3 class=\"sub-title1\">Technical Information</h3>\n <div\n class=\"\n ProductDescriptionContainer\n product-droppdown__section-description\n \"\n >\n <div class=\"product-tech-info\">\n <div class=\"product-tech-info__line-item\">\n <div class=\"product-tech-info__line-item-left\">\n <b>Immunogen</b>\n </div>\n <div class=\"product-tech-info__line-item-right\">\n A synthetic peptide corresponding to human BRD4 amino acids 1312\n to 1362.\n </div>\n </div>\n <div class=\"product-tech-info__line-item\">\n <div class=\"product-tech-info__line-item-left\">\n <b>Formulation</b>\n </div>\n <div class=\"product-tech-info__line-item-right\">\n Antigen affinity-purified antibody (1 mg/mL) in\n Tris-citrate/phosphate buffer pH 7 to 8, 0.09% sodium azide.\n </div>\n </div>\n <div class=\"product-tech-info__line-item\">\n <div class=\"product-tech-info__line-item-left\">\n <b>Storage and Stability</b>\n </div>\n <div class=\"product-tech-info__line-item-right\">\n Stable for 1 year at 4°C from date of receipt.\n </div>\n </div>\n </div>\n </div>\n </div>\n </div>\n <div id=\"prodAccordion\">\n <div id=\"ProductDescription\" class=\"Block Panel\">\n <h3 class=\"sub-title1\">Application Notes</h3>\n <div\n class=\"\n ProductDescriptionContainer\n product-droppdown__section-description\n \"\n >\n <p><strong>Recommended Dilutions:</strong></p>\n <p><strong>CUT&RUN:</strong> 0.5 µg</p>\n <p><strong>Western Blot (WB):</strong> 1:2,000 - 1:10,000</p>\n <p><strong>Immunoprecipitation (IP):</strong> 2 - 5 µg/mg lysate</p>\n <p><strong>Immunohistochemistry (IHC):</strong> 1:1,000 - 1:5,000*</p>\n <p>\n <em\n >*Epitope retrieval with citrate buffer pH 6.0 recommended for\n FFPE tissue</em\n >\n </p>\n </div>\n </div>\n </div>\n <div id=\"prodAccordion\">\n <div id=\"ProductDescription\" class=\"Block Panel\">\n <h3 class=\"sub-title1\">References</h3>\n <div\n class=\"\n ProductDescriptionContainer\n product-droppdown__section-description\n \"\n >\n <strong>Background References:</strong>\n <br />\n Conrad et al. <em>Mol. Cell </em> (2017). PMID:\n <a\n href=\"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5610089/\"\n title=\"The Short Isoform of BRD4 Promotes HIV-1 Latency by Engaging Repressive SWI/SNF Chromatin Remodeling Complexes\"\n target=\"new\"\n >\n 28844864</a\n ><br />\n </div>\n </div>\n </div>\n <div id=\"prodAccordion\">\n <div id=\"ProductDescription\" class=\"Block Panel\">\n <h3 class=\"sub-title1\">Documents & Resources</h3>\n <div\n class=\"\n ProductDescriptionContainer\n product-droppdown__section-description\n \"\n >\n <div class=\"product-documents\">\n <a\n href=\"/content/documents/tds/13-2003.pdf\"\n target=\"_new\"\n class=\"product-documents__link\"\n >\n <svg\n version=\"1.1\"\n id=\"Layer_1\"\n xmlns=\"http://www.w3.org/2000/svg\"\n xmlns:xlink=\"http://www.w3.org/1999/xlink\"\n x=\"0px\"\n y=\"0px\"\n viewBox=\"0 0 228 240\"\n style=\"enable-background: new 0 0 228 240\"\n xml:space=\"preserve\"\n class=\"product-documents__icon\"\n >\n <g>\n <path\n class=\"product-documents__svg-pdf\"\n d=\"M191.92,68.77l-47.69-47.69c-1.33-1.33-3.12-2.08-5.01-2.08H45.09C41.17,19,38,22.17,38,26.09v184.36\n c0,3.92,3.17,7.09,7.09,7.09h141.82c3.92,0,7.09-3.17,7.09-7.09V73.8C194,71.92,193.25,70.1,191.92,68.77z M177.65,77.06h-41.7\n v-41.7L177.65,77.06z M178.05,201.59H53.95V34.95h66.92v47.86c0,5.14,4.17,9.31,9.31,9.31h47.86V201.59z\"\n />\n </g>\n <rect\n x=\"20\"\n y=\"112\"\n class=\"product-documents__svg-background\"\n width=\"146\"\n height=\"76\"\n />\n <g>\n <path\n class=\"product-documents__svg-pdf\"\n d=\"M23.83,125.68h22.36c5.29,0,9.41,1.33,12.35,4c2.94,2.67,4.42,6.39,4.42,11.18c0,4.78-1.47,8.51-4.42,11.18\n c-2.94,2.67-7.06,4-12.35,4H34.59v18.29H23.83V125.68z M44.81,147.9c5.38,0,8.07-2.32,8.07-6.97c0-2.39-0.67-4.16-2-5.31\n c-1.33-1.15-3.36-1.73-6.07-1.73H34.59v14.01H44.81z\"\n />\n <path\n class=\"product-documents__svg-pdf\"\n d=\"M69.92,125.68h18.91c5.29,0,9.84,0.97,13.66,2.9c3.82,1.93,6.74,4.72,8.76,8.35\n c2.02,3.63,3.04,7.98,3.04,13.04c0,5.06-1,9.42-3,13.08c-2,3.66-4.91,6.45-8.73,8.38c-3.82,1.93-8.4,2.9-13.73,2.9H69.92V125.68z\n M88.07,165.63c10.35,0,15.52-5.22,15.52-15.66c0-10.4-5.17-15.59-15.52-15.59h-7.38v31.26H88.07z\"\n />\n <path\n class=\"product-documents__svg-pdf\"\n d=\"M122.57,125.68h32.84v8.49h-22.22v11.18h20.84v8.49h-20.84v20.49h-10.63V125.68z\"\n />\n </g>\n </svg>\n <span class=\"product-documents__info\">Technical Datasheet</span>\n </a>\n </div>\n </div>\n </div>\n </div>\n <div id=\"prodAccordion\">\n <div id=\"ProductDescription\" class=\"Block Panel\">\n <h3 class=\"sub-title1\">Additional Info</h3>\n <div\n class=\"\n ProductDescriptionContainer\n product-droppdown__section-description\n \"\n >\n <p>\n This product is provided for commercial sale under license from\n Bethyl Laboratories, Inc.\n </p>\n\n <strong>Applications Key:</strong> <br /><br />\n\n ChIP: Chromatin immunoprecipitation<br />\n CUT&RUN: Cleavage Under Targets and Release Using Nuclease<br />\n CUT&Tag: Cleavage Under Targets and Tagmentation<br />\n E: ELISA<br />\n FACS: Flow cytometry<br />\n ICC: Immunocytochemistry<br />\n IF: Immunofluorescence<br />\n IHC: Immunohistochemistry<br />\n IP: Immunoprecipitation<br />\n L: Luminex<br />\n WB: Western Blot<br /><br />\n\n <strong>Reactivity Key:</strong> <br /><br />\n\n B: Bovine<br />\n Ce: <em>C. elegans</em><br />\n Ch: Chicken<br />\n Dm: <em>Drosophila</em><br />\n Eu: Eukaryote<br />\n H: Human<br />\n M: Mouse<br />\n Ma: Mammal<br />\n R: Rat<br />\n Sc: <em>S. cerevisiae</em><br />\n Sp: <em>S. pombe</em><br />\n WR: Wide Range (predicted)<br />\n X: Xenopus<br />\n Z: Zebrafish<br />\n </div>\n </div>\n </div>\n </div>\n</div>\n","detail_messages":"","gift_wrapping_available":false,"gtin":null,"id":"798","images":[{"alt":"BRD4 CUTANA CUTandRUN Antibody","data":"https://cdn11.bigcommerce.com/s-y9o92/images/stencil/{:size}/products/798/933/brd4-cutana-cutandrun-antibody__13949.1645734524.jpg?c=2"}],"main_image":{"alt":"BRD4 CUTANA CUTandRUN Antibody","data":"https://cdn11.bigcommerce.com/s-y9o92/images/stencil/{:size}/products/798/933/brd4-cutana-cutandrun-antibody__13949.1645734524.jpg?c=2"},"max_purchase_quantity":0,"meta_description":"Rabbit polyclonal anti-BRD4 antibody validated for CUT&RUN, WB, IP, IHC. Rigorously tested for reliable and robust performance in CUT&RUN assays.","meta_keywords":"brd4 antibody, CUT&RUN antibody","min_purchase_quantity":0,"mpn":null,"num_reviews":0,"options":[],"page_title":"BRD4 Antibody | CUTANA™ CUT&RUN Compatible","price":{"tax_label":"Sales Tax","without_tax":{"currency":"USD","formatted":"$445.00","value":445}},"rating":0,"related_products":[{"add_to_cart_url":"https://www.epicypher.com/cart.php?action=add&product_id=804","add_to_wishlist_url":"/wishlist.php?action=add&product_id=804","availability":"","brand":{"name":null},"category":["Antibodies","Antibodies/CUTANA™ CUT&RUN Antibodies","Antibodies/CUTANA™ CUT&RUN Antibodies/CUT&RUN Antibodies - Chromatin-Associated Proteins","Epigenetics Kits and Reagents/CUTANA™ ChIC / CUT&RUN Assays"],"custom_fields":[{"id":666,"name":"Pack Size","value":"100 µL"}],"date_added":"28th Jan 2021","demo":false,"has_options":false,"id":804,"image":{"alt":"CHD1 CUTANA™ CUT&RUN Antibody","data":"https://cdn11.bigcommerce.com/s-y9o92/images/stencil/{:size}/products/804/793/2020.12.08_CUTANA_ChAPs_Antibody_thumbnail_RGB_LS__39482.1614276466.png?c=2"},"images":[{"alt":"CHD1 CUTANA™ CUT&RUN Antibody","data":"https://cdn11.bigcommerce.com/s-y9o92/images/stencil/{:size}/products/804/793/2020.12.08_CUTANA_ChAPs_Antibody_thumbnail_RGB_LS__39482.1614276466.png?c=2"}],"low_stock_level":null,"name":"CHD1 CUTANA™ CUT&RUN Antibody","num_reviews":null,"pre_order":false,"price":{"tax_label":"Sales Tax","without_tax":{"currency":"USD","formatted":"$445.00","value":445}},"qty_in_cart":0,"rating":null,"show_cart_action":true,"sku":"13-2008","stock_level":null,"summary":"\n \n \n Type: Polyclonal\n \n \n Host: Rabbit\n \n \n Target Size: 197 kD","url":"https://www.epicypher.com/products/antibodies/chd1-cutana-cut-run-antibody","weight":{"formatted":"0.01 LBS","value":0.01}},{"add_to_wishlist_url":"/wishlist.php?action=add&product_id=874","availability":"","brand":{"name":null},"category":["Antibodies/CUTANA™ CUT&RUN Antibodies","Antibodies/CUTANA™ CUT&RUN Antibodies/CUT&RUN Antibodies - Chromatin-Associated Proteins","Epigenetics Kits and Reagents/CUTANA™ ChIC / CUT&RUN Assays"],"custom_fields":[{"id":908,"name":"Pack Size","value":"100 µL"}],"date_added":"20th Jul 2021","demo":false,"has_options":false,"id":874,"image":{"alt":"CTCF CUTANA CUTandRUN Antibody","data":"https://cdn11.bigcommerce.com/s-y9o92/images/stencil/{:size}/products/874/968/ctcf-cutana-cutandrun-antibody__00748.1645734560.jpg?c=2"},"images":[{"alt":"CTCF CUTANA CUTandRUN Antibody","data":"https://cdn11.bigcommerce.com/s-y9o92/images/stencil/{:size}/products/874/968/ctcf-cutana-cutandrun-antibody__00748.1645734560.jpg?c=2"}],"low_stock_level":null,"name":"CTCF CUTANA™ CUT&RUN Antibody","num_reviews":null,"out_of_stock_message":false,"pre_order":false,"price":{"tax_label":"Sales Tax","without_tax":{"currency":"USD","formatted":"$445.00","value":445}},"qty_in_cart":0,"rating":null,"show_cart_action":true,"sku":"13-2014","stock_level":null,"summary":"\n \n \n Type: Monoclonal [BLR041F]\n \n \n Host: Rabbit\n \n \n Target Size: ","url":"https://www.epicypher.com/products/antibodies/cutana-cut-run-compatible-antibodies/ctcf-cutana-cut-and-run-antibody","weight":{"formatted":"0.00 LBS","value":0}},{"add_to_cart_url":"https://www.epicypher.com/cart.php?action=add&product_id=906","add_to_wishlist_url":"/wishlist.php?action=add&product_id=906","availability":"","brand":{"name":null},"category":["Antibodies/CUTANA™ CUT&RUN Antibodies","Antibodies/CUTANA™ CUT&RUN Antibodies/CUT&RUN Antibodies - Chromatin-Associated Proteins","Epigenetics Kits and Reagents/CUTANA™ ChIC / CUT&RUN Assays"],"custom_fields":[{"id":1030,"name":"Pack Size","value":"100 µL"}],"date_added":"30th Mar 2022","demo":false,"has_options":false,"id":906,"image":{"alt":"Menin CUTANA™ CUT&RUN Antibody","data":"https://cdn11.bigcommerce.com/s-y9o92/images/stencil/{:size}/products/906/1010/chd3-cutana-cutandrun-antibody__66975.1645734525__91145.1648666822__40012.1648670522.jpg?c=2"},"images":[{"alt":"Menin CUTANA™ CUT&RUN Antibody","data":"https://cdn11.bigcommerce.com/s-y9o92/images/stencil/{:size}/products/906/1010/chd3-cutana-cutandrun-antibody__66975.1645734525__91145.1648666822__40012.1648670522.jpg?c=2"}],"low_stock_level":null,"name":"Menin CUTANA™ CUT&RUN Antibody","num_reviews":null,"pre_order":false,"price":{"tax_label":"Sales Tax","without_tax":{"currency":"USD","formatted":"$445.00","value":445}},"qty_in_cart":0,"rating":null,"show_cart_action":true,"sku":"13-2021","stock_level":null,"summary":"\n \n \n Type: Polyclonal\n \n \n Host: Rabbit\n \n \n Applications: CUT&R","url":"https://www.epicypher.com/products/antibodies/cutana-cut-run-antibodies/cut-run-antibodies-chromatin-associated-proteins/menin-cutana-cut-run-antibody","weight":{"formatted":"0.01 LBS","value":0.01}},{"add_to_cart_url":"https://www.epicypher.com/cart.php?action=add&product_id=907","add_to_wishlist_url":"/wishlist.php?action=add&product_id=907","availability":"","brand":{"name":null},"category":["Antibodies/CUTANA™ CUT&RUN Antibodies","Antibodies/CUTANA™ CUT&RUN Antibodies/CUT&RUN Antibodies - Chromatin-Associated Proteins","Epigenetics Kits and Reagents/CUTANA™ ChIC / CUT&RUN Assays"],"custom_fields":[{"id":1029,"name":"Pack Size","value":"100 µL"}],"date_added":"30th Mar 2022","demo":false,"has_options":false,"id":907,"image":{"alt":"ELF1 CUTANA™ CUT&RUN Antibody","data":"https://cdn11.bigcommerce.com/s-y9o92/images/stencil/{:size}/products/907/1009/chd3-cutana-cutandrun-antibody__66975.1645734525__91145.1648666822__83973.1648670498.jpg?c=2"},"images":[{"alt":"ELF1 CUTANA™ CUT&RUN Antibody","data":"https://cdn11.bigcommerce.com/s-y9o92/images/stencil/{:size}/products/907/1009/chd3-cutana-cutandrun-antibody__66975.1645734525__91145.1648666822__83973.1648670498.jpg?c=2"}],"low_stock_level":null,"name":"ELF1 CUTANA™ CUT&RUN Antibody","num_reviews":null,"pre_order":false,"price":{"tax_label":"Sales Tax","without_tax":{"currency":"USD","formatted":"$445.00","value":445}},"qty_in_cart":0,"rating":null,"show_cart_action":true,"sku":"13-2023","stock_level":null,"summary":"\n \n \n Type: Polyclonal\n \n \n Host: Rabbit\n \n \n Applications: CUT&R","url":"https://www.epicypher.com/products/antibodies/cutana-cut-run-antibodies/cut-run-antibodies-chromatin-associated-proteins/elf1-cutana-cut-run-antibody","weight":{"formatted":"0.01 LBS","value":0.01}},{"add_to_cart_url":"https://www.epicypher.com/cart.php?action=add&product_id=909","add_to_wishlist_url":"/wishlist.php?action=add&product_id=909","availability":"","brand":{"name":null},"category":["Antibodies/CUTANA™ CUT&RUN Antibodies","Antibodies/CUTANA™ CUT&RUN Antibodies/CUT&RUN Antibodies - Chromatin-Associated Proteins","Epigenetics Kits and Reagents/CUTANA™ ChIC / CUT&RUN Assays"],"custom_fields":[{"id":1032,"name":"Pack Size","value":"100 µL"}],"date_added":"30th Mar 2022","demo":false,"has_options":false,"id":909,"image":{"alt":"EZH2 CUTANA™ CUT&RUN Antibody","data":"https://cdn11.bigcommerce.com/s-y9o92/images/stencil/{:size}/products/909/1007/chd3-cutana-cutandrun-antibody__66975.1645734525__91145.1648666822__81008.1648670468.jpg?c=2"},"images":[{"alt":"EZH2 CUTANA™ CUT&RUN Antibody","data":"https://cdn11.bigcommerce.com/s-y9o92/images/stencil/{:size}/products/909/1007/chd3-cutana-cutandrun-antibody__66975.1645734525__91145.1648666822__81008.1648670468.jpg?c=2"}],"low_stock_level":null,"name":"EZH2 CUTANA™ CUT&RUN Antibody","num_reviews":null,"pre_order":false,"price":{"tax_label":"Sales Tax","without_tax":{"currency":"USD","formatted":"$445.00","value":445}},"qty_in_cart":0,"rating":null,"show_cart_action":true,"sku":"13-2026","stock_level":null,"summary":"\n \n \n Type: Polyclonal\n \n \n Host: Rabbit\n \n \n Applications: CUT&R","url":"https://www.epicypher.com/products/antibodies/cutana-cut-run-antibodies/cut-run-antibodies-chromatin-associated-proteins/ezh2-cutana-cut-run-antibody","weight":{"formatted":"0.01 LBS","value":0.01}}],"shipping":{"calculated":true},"shipping_messages":[],"show_quantity_input":1,"sku":"13-2003","tags":[],"title":"BRD4 CUTANA™ CUT&RUN Antibody","upc":null,"url":"https://www.epicypher.com/products/antibodies/brd4-cutana-cut-run-antibody","warranty":"","weight":"0.01 LBS"} Pack size: 50 µL
- Type: Polyclonal
- Host: Rabbit
- Target Size: 152 kDa
- Format: Affinity Purified IgG
- Reactivity: Human, Mouse
- Applications: CUT&RUN, WB, IP, IHC
Description
This antibody meets EpiCypher’s “CUTANA Compatible” criteria for performance in Cleavage Under Targets and Release Using Nuclease (CUT&RUN) and/or Cleavage Under Targets and Tagmentation (CUT&Tag) approaches to genomic mapping. Every lot of a CUTANA Compatible antibody is tested in the indicated CUTANA approach using EpiCypher optimized protocols and determined to yield peaks that show a genomic distribution pattern consistent with reported function(s) of the target protein. BRD4 antibody produces CUT&RUN peaks primarily flanking transcription start sites (TSSs, Figure 1). BRD4 peaks show a large degree of overlap with BRG1/SMARCA4 peaks (Figure 2), as has been reported in the literature (Conrad et al., 2017).
Validation Data
Figure 1: BRD4 enrichment at annotated TSSs in
CUT&RUN
CUT&RUN was performed using 500,000 K562 cells with BRD4
antibody as well as control antibodies (IgG negative
control, EpiCypher
13-0042; H3K4me3 positive control, EpiCypher
13-0041). Sequencing reads were aligned to annotated TSSs (+/- 2
kbp) of 18,793 genes. High, medium, and low signal is ranked
by intensity (top to bottom) and reflected by red, yellow,
and blue colors, respectively. All rows aligned relative to
H3K4me3 antibody.
Figure 2: BRD4 CUT&RUN peak enrichment and functional
overlap
The CUT&RUN data from Figure 1 was subjected to peak calling
using MACS2. BRD4 peaks overlapped with BRG1/ SMARCA4
antibody CUT&RUN peaks (EpiCypher
13-2002, top), as has been demonstrated in the literature (Conrad
et al., 2017). Three representative loci show BRD4 peaks in
relation to control and BRG1 antibodies (bottom, Integrative
Genomics Viewer).
Figure 3: Western blot detection of human BRD4
Whole cell lysates were isolated from HeLa, HEK293T
(“293T”), Jurkat, MB231, Hep-G2, A-549, SW620, SK-MEL-28
(“SK”), and Ramos cells using NETN lysis buffer. Lysates (15
µg) were loaded onto 4-8% SDS-PAGE gel and analyzed under
standard western blot conditions using BRD4 antibody
(1:1,000 dilution).
Figure 4: Western blot detection of mouse BRD4
Whole cell lysates were isolated from NIH 3T3 (“3T3”) cells
using NETN lysis buffer. Lysates (15 µg) were loaded onto
4-8% SDS-PAGE gel and analyzed under standard western blot
conditions using BRD4 antibody (0.1 µg/mL).
Figure 5: Immunoprecipitation of human BRD4
EpiCypher BRD4 antibody (3 µg) was used to immunoprecipitate
whole cell lysates isolated from HeLa cells using NETN lysis
buffer (1 mg per IP). A negative control IgG antibody and
positive control antibodies to a different BRD4 epitopes
(Bethyl Laboratories) were also used to demonstrate
specificity of the IP. Immunoprecipitates were loaded onto
4-8% SDS-PAGE gel (20% of IP loaded) and probed via western
blot with a different BRD4 antibody (Bethyl Laboratories,
Catalog No. A700-004, 1:1,000).
Figure 6: Immunohistochemistry detection of human and
mouse BRD4
(A) FFPE section of human ovarian carcinoma
examined using BRD4 antibody (1:5,000 dilution, 0.2 µg/mL).
(B) FFPE section of mouse CT26 colon
carcinoma examined using BRD4 antibody (1:1,000 dilution, 1
µg/mL).
Technical Information
Application Notes
Recommended Dilutions:
CUT&RUN: 0.5 µg
Western Blot (WB): 1:2,000 - 1:10,000
Immunoprecipitation (IP): 2 - 5 µg/mg lysate
Immunohistochemistry (IHC): 1:1,000 - 1:5,000*
*Epitope retrieval with citrate buffer pH 6.0 recommended for FFPE tissue
References
Documents & Resources
Additional Info
This product is provided for commercial sale under license from Bethyl Laboratories, Inc.
Applications Key:ChIP: Chromatin immunoprecipitation
CUT&RUN: Cleavage Under Targets and Release Using Nuclease
CUT&Tag: Cleavage Under Targets and Tagmentation
E: ELISA
FACS: Flow cytometry
ICC: Immunocytochemistry
IF: Immunofluorescence
IHC: Immunohistochemistry
IP: Immunoprecipitation
L: Luminex
WB: Western Blot
Reactivity Key:
B: Bovine
Ce: C. elegans
Ch: Chicken
Dm: Drosophila
Eu: Eukaryote
H: Human
M: Mouse
Ma: Mammal
R: Rat
Sc: S. cerevisiae
Sp: S. pombe
WR: Wide Range (predicted)
X: Xenopus
Z: Zebrafish