HA Tag CUTANA™ CUT&RUN Antibody
{"url":"https://www.epicypher.com/products/antibodies/cutana-cut-run-compatible-antibodies/ha-tag-cutana-cut-and-run-antibody","add_this":[{"service":"facebook","annotation":""},{"service":"email","annotation":""},{"service":"print","annotation":""},{"service":"twitter","annotation":""},{"service":"linkedin","annotation":""}],"gtin":null,"id":"875","bulk_discount_rates":[],"can_purchase":true,"meta_description":"Rabbit polyclonal HA Tag antibody validated for CUT&RUN and WB. Rigorously tested for reliable and robust performance in CUT&RUN assays.","category":["Antibodies/CUTANA™ CUT&RUN Antibodies","Antibodies/CUTANA™ CUT&RUN Antibodies/CUT&RUN Antibodies - Chromatin-Associated Proteins","Epigenetics Kits and Reagents/CUTANA™ ChIC / CUT&RUN Assays"],"AddThisServiceButtonMeta":"","main_image":{"data":"https://cdn11.bigcommerce.com/s-y9o92/images/stencil/{:size}/products/875/937/ha-tag-cutana-cutandrun-antibody__43540.1645734529.jpg?c=2","alt":"HA Tag CUTANA CUTandRUN Antibody"},"add_to_wishlist_url":"/wishlist.php?action=add&product_id=875","shipping":{"calculated":true},"num_reviews":0,"weight":"0.00 LBS","custom_fields":[{"id":"909","name":"Pack Size","value":"100 µg"}],"sku":"13-2010","description":"<div class=\"product-general-info\">\n <ul class=\"product-general-info__list-left\">\n <li class=\"product-general-info__list-item\">\n <strong>Type: </strong>Polyclonal\n </li>\n <li class=\"product-general-info__list-item\">\n <strong>Host: </strong>Rabbit\n </li>\n <li class=\"product-general-info__list-item\">\n <strong>Applications: </strong>CUT&RUN, WB\n </li>\n </ul>\n <ul class=\"product-general-info__list-right\">\n <li class=\"product-general-info__list-item\">\n <strong>Reactivity: </strong>HA Epitope (YPYDVPDYA)\n </li>\n <li class=\"product-general-info__list-item\">\n <strong>Format: </strong>Antigen affinity-purified\n </li>\n <li class=\"product-general-info__list-item\">\n <strong>Target Size: </strong>N/A\n </li>\n </ul>\n <ul style=\"padding: 0\" class=\"product-general-info__list-right\">\n <li class=\"product-general-info__list-item\">\n <a href=\"#bioz\">\n <div id=\"w-s-3835-13-2010\" style=\"\n width: 240px;\n height: 58px;\n position: relative;\n overflow-y: hidden;\n \"></div>\n <div id=\"bioz-w-pb-13-2010-div\" style=\"width: 240px\">\n <a id=\"bioz-w-pb-13-2010\" style=\"font-size: 12px; color: transparent\" href=\"https://www.bioz.com/\"\n target=\"_blank\">\n <img src=\"https://cdn.bioz.com/assets/favicon.png\" style=\"\n width: 11px;\n height: 11px;\n vertical-align: baseline;\n padding-bottom: 0px;\n margin-left: 0px;\n margin-bottom: 0px;\n float: none;\n display: none;\n \" />\n Powered by Bioz\n </a>\n </div>\n </a>\n </li>\n </ul>\n</div>\n<div class=\"service_accordion product-droppdown\">\n <div class=\"container\">\n <div id=\"prodAccordion\">\n <div id=\"ProductDescription\" class=\"Block Panel current\">\n <h3 class=\"sub-title1\">Description</h3>\n <div class=\"ProductDescriptionContainer product-droppdown__section-description-specific\">\n <p>\n This antibody meets EpiCypher’s \"CUTANA Compatible\" criteria\n for performance in Cleavage Under Targets and Release Using\n Nuclease (CUT&RUN) and/or Cleavage Under Targets and\n Tagmentation (CUT&Tag) approaches to genomic mapping. Every\n lot of a CUTANA Compatible antibody is tested in the indicated\n approach using\n <a href=\"/protocols\">EpiCypher optimized protocols</a> and\n determined to yield peaks that show a genomic distribution\n pattern consistent with reported function(s) of the target. HA\n antibody is useful for studies utilizing HA-tagged target\n proteins. HA Tag antibody produces CUT&RUN peaks (<strong>Figure 2</strong>) in breast cancer cells expressing 3xHA-tagged GATA3 transcription factor [<a href=\"https://pubmed.ncbi.nlm.nih.gov/26922637/\">Takaku et al., 2016</a>]*.\n </p>\n <p style=\"\n background-color: #4698cb;\n color: #fff;\n padding: 1.3rem;\n text-align: center;\n border-radius: 12px;\n \">\n <a target=\"_blank\" style=\"color: #fff;\" href=\"/products/nucleosomes/snap-cutana-ha-tag-panel\">Pair with our\n SNAP-CUTANA™ HA Tag Panel for CUT&RUN success. Check it out here.\n </a>\n </p>\n <i>\n *Thanks to Dr. Takaku (UND) for 3xFlag-GATA3-3xHA MDA-MB-231\n cells.\n </i>\n </div>\n </div>\n </div>\n <div id=\"prodAccordion\">\n <div id=\"ProductDescription\" class=\"Block Panel current\">\n <h3 class=\"sub-title1\">Validation Data</h3>\n <div class=\"ProductDescriptionContainer product-droppdown__section-description-specific\">\n <section class=\"image-picker\">\n <div class=\"image-picker__left\">\n <div class=\"image-picker__main-content_active image-picker__main-content\">\n <div class=\"image-picker__header-content\">\n <button class=\"image-picker__left-arrow\">\n <svg class=\"image-picker__svg-left\" width=\"24\" height=\"24\" viewBox=\"0 0 24 24\">\n <path d=\"M16.67 0l2.83 2.829-9.339 9.175 9.339 9.167-2.83 2.829-12.17-11.996z\" />\n </svg>\n </button>\n <a href=\"/content/images/products/antibodies/13-2010-spike-ins-assay-control.jpeg\" target=\"_new\"\n class=\"image-picker__main-image-link\"><img loading=\"lazy\" alt=\"13-2010-spike-ins-assay-control\"\n src=\"/content/images/products/antibodies/13-2010-spike-ins-assay-control.jpeg\"\n class=\"image-picker__main-image\" />\n <span class=\"image-picker__main-image-caption\">(Click to enlarge)</span></a>\n <button class=\"image-picker__right-arrow\">\n <svg class=\"image-picker__svg-right\" width=\"24\" height=\"24\" viewBox=\"0 0 24 24\">\n <path d=\"M7.33 24l-2.83-2.829 9.339-9.175-9.339-9.167 2.83-2.829 12.17 11.996z\" />\n </svg>\n </button>\n </div>\n <p>\n <span class=\"image-picker__span-content\"><strong>Figure 1: Defined nucleosome spike-ins provide\n in-assay control for HA Tag antibody in CUT&RUN </strong><br />\n CUT&RUN sequencing reads were aligned to the unique DNA barcodes corresponding to each nucleosome in\n the SNAP-CUTANA™ HA Tag Panel (EpiCypher <a href=\"/products/nucleosomes/snap-cutana-ha-tag-panel\"\n target=\"_blank\">19-5002</a>). Data are expressed as a percent relative to\n on-target recovery (HA Tag set to 100%) or total counts (IgG/GATA3). IgG/GATA3 show no preferential\n binding to unmodified or HA spike-in nucleosomes. HA Tag antibody selectively enriches the HA Tag\n spike-in nucleosome, validating the antibody in CUT&RUN.\n </span>\n </p>\n </div>\n <div class=\"image-picker__main-content\">\n <div class=\"image-picker__header-content\">\n <button class=\"image-picker__left-arrow\">\n <svg class=\"image-picker__svg-left\" width=\"24\" height=\"24\" viewBox=\"0 0 24 24\">\n <path d=\"M16.67 0l2.83 2.829-9.339 9.175 9.339 9.167-2.83 2.829-12.17-11.996z\" />\n </svg>\n </button>\n <a href=\"/content/images/products/antibodies/13-2010-ha-tagged-protein.jpeg\" target=\"_new\"\n class=\"image-picker__main-image-link\"><img loading=\"lazy\" alt=\"13-2010-ha-tagged-protein\"\n src=\"/content/images/products/antibodies/13-2010-ha-tagged-protein-thumb.jpeg\"\n class=\"image-picker__main-image\" />\n <span class=\"image-picker__main-image-caption\">(Click to enlarge)</span></a>\n <button class=\"image-picker__right-arrow\">\n <svg class=\"image-picker__svg-right\" width=\"24\" height=\"24\" viewBox=\"0 0 24 24\">\n <path d=\"M7.33 24l-2.83-2.829 9.339-9.175-9.339-9.167 2.83-2.829 12.17 11.996z\" />\n </svg>\n </button>\n </div>\n <p>\n <span class=\"image-picker__span-content\"><strong>Figure 2: HA-tagged protein peaks in\n CUT&RUN</strong><br />\n Heatmaps show HA Tag antibody-enriched peaks called for GATA3-3xHA overexpressing cells (OE) in\n aligned rows relative to all other experimental conditions. Rows are ranked by intensity (top to\n bottom) and colored such that red indicates high localized enrichment and blue denotes background\n signal. A high degree of overlap is observed for HA and GATA3 antibodies as expected in the OE\n cells, while empty vector control (Ctrl) shows absence of HA and lower GATA3 enrichment representing\n endogenous protein. IgG shows low background signal. H3K4me3, a canonical mark of promoters, does\n not appear in regions of high GATA3 enrichment.\n </span>\n </p>\n </div>\n\n <div class=\"image-picker__main-content\">\n <div class=\"image-picker__header-content\">\n <button class=\"image-picker__left-arrow\">\n <svg class=\"image-picker__svg-left\" width=\"24\" height=\"24\" viewBox=\"0 0 24 24\">\n <path d=\"M16.67 0l2.83 2.829-9.339 9.175 9.339 9.167-2.83 2.829-12.17-11.996z\" />\n </svg>\n </button>\n <a href=\"/content/images/products/antibodies/13-2010-browser-tracks.jpeg\" target=\"_new\"\n class=\"image-picker__main-image-link\"><img loading=\"lazy\" alt=\"13-2010-browser-tracks\"\n src=\"/content/images/products/antibodies/13-2010-browser-tracks.jpeg\"\n class=\"image-picker__main-image\" />\n <span class=\"image-picker__main-image-caption\">(Click to enlarge)</span></a>\n <button class=\"image-picker__right-arrow\">\n <svg class=\"image-picker__svg-right\" width=\"24\" height=\"24\" viewBox=\"0 0 24 24\">\n <path d=\"M7.33 24l-2.83-2.829 9.339-9.175-9.339-9.167 2.83-2.829 12.17 11.996z\" />\n </svg>\n </button>\n </div>\n <p>\n <span class=\"image-picker__span-content\"><strong>Figure 3: CUT&RUN representative browser tracks for\n HA-tagged protein</strong><br />\n Gene browser shows were generated using the Integrative Genomics Viewer (IGV, Broad Institute). Two\n of the top called peaks in GATA3-3xHA overexpressing (OE) cells are shown. The peaks show the same\n distribution patterns as observed in the genome-wide heatmaps (Figure 2). IgG shows low background,\n H3K4me3 is unchanged between empty vector control (Ctrl) and OE cells, GATA3 peaks are more robust\n in OE cells, and peaks overlap between GATA3 and HA antibodies in OE cells. These results\n demonstrate the robustness and specificity of HA Tag antibody in CUT&RUN experiments targeting\n HA-tagged proteins.\n </span>\n </p>\n </div>\n <div class=\"image-picker__main-content\">\n <div class=\"image-picker__header-content\">\n <button class=\"image-picker__left-arrow\">\n <svg class=\"image-picker__svg-left\" width=\"24\" height=\"24\" viewBox=\"0 0 24 24\">\n <path d=\"M16.67 0l2.83 2.829-9.339 9.175 9.339 9.167-2.83 2.829-12.17-11.996z\" />\n </svg>\n </button>\n <a href=\"/content/images/products/antibodies/13-2010-western-blot.jpeg\" target=\"_new\"\n class=\"image-picker__main-image-link\"><img loading=\"lazy\" alt=\"13-2010-western-blot\"\n src=\"/content/images/products/antibodies/13-2010-western-blot.jpeg\"\n class=\"image-picker__main-image\" />\n <span class=\"image-picker__main-image-caption\">(Click to enlarge)</span></a>\n <button class=\"image-picker__right-arrow\">\n <svg class=\"image-picker__svg-right\" width=\"24\" height=\"24\" viewBox=\"0 0 24 24\">\n <path d=\"M7.33 24l-2.83-2.829 9.339-9.175-9.339-9.167 2.83-2.829 12.17 11.996z\" />\n </svg>\n </button>\n </div>\n <p>\n <span class=\"image-picker__span-content\"><strong>Figure 4: Western blot data</strong><br />\n <em>E. coli</em> cells expressing a multi-tag fusion protein were used to prepare whole cell lysates. The\n indicated amounts (ng) of lysate were loaded onto a 4-20% SDS-PAGE gel and analyzed under standard\n western blot conditions using HA Tag antibody at a dilution of 1:25,000.\n </span>\n </p>\n </div>\n <div class=\"image-picker__main-content\">\n <div class=\"image-picker__header-content\">\n <button class=\"image-picker__left-arrow\">\n <svg class=\"image-picker__svg-left\" width=\"24\" height=\"24\" viewBox=\"0 0 24 24\">\n <path d=\"M16.67 0l2.83 2.829-9.339 9.175 9.339 9.167-2.83 2.829-12.17-11.996z\" />\n </svg>\n </button>\n <a href=\"https://cdn11.bigcommerce.com/s-y9o92/images/stencil/original/image-manager/2022.07.06-c-r-workflow-simple-rgb-ew.png?t=1697220982&_gl=1*u66xe1*_ga*NDc4MDE2MzAxLjE2OTY2MjA3NTU.*_ga_WS2VZYPC6G*MTY5NzY5NzkxNi4zNi4xLjE2OTc2OTk4OTEuNTYuMC4w\"\n target=\"_new\" class=\"image-picker__main-image-link\"><img loading=\"lazy\" alt=\"13_2012_IP\"\n src=\"https://cdn11.bigcommerce.com/s-y9o92/images/stencil/original/image-manager/2022.07.06-c-r-workflow-simple-rgb-ew.png?t=1697220982&_gl=1*u66xe1*_ga*NDc4MDE2MzAxLjE2OTY2MjA3NTU.*_ga_WS2VZYPC6G*MTY5NzY5NzkxNi4zNi4xLjE2OTc2OTk4OTEuNTYuMC4w\"\n class=\"image-picker__main-image\" />\n <span class=\"image-picker__main-image-caption\">(Click to enlarge)</span></a>\n <button class=\"image-picker__right-arrow\">\n <svg class=\"image-picker__svg-right\" width=\"24\" height=\"24\" viewBox=\"0 0 24 24\">\n <path d=\"M7.33 24l-2.83-2.829 9.339-9.175-9.339-9.167 2.83-2.829 12.17 11.996z\" />\n </svg>\n </button>\n </div>\n <p>\n <span class=\"image-picker__span-content\"><strong>Figure 5: CUT&RUN Methods</strong><br />\n CUT&RUN was performed on 500k MDA-MB-231 native cells stably overexpressing c-terminal 3xHA-tagged\n GATA3 [1] or containing vector control. 0.5 µg of either HA Tag, H3K4me3 (EpiCypher <a href=\"/products/antibodies/h3k4me3-antibody-snap-certified-for-cut-run-and-cut-tag\" target=\"_blank\">13-0060</a>), GATA3\n (CST 5852), or IgG (EpiCypher <a href=\"/products/nucleosomes/snap-cutana-spike-in-controls/cutana-igg-negative-control-antibody-for-cut-run-and-cut-tag\" target=\"_blank\">13-0042</a>) antibodies were used with the CUTANA™ ChIC/CUT&RUN Kit v4\n (EpiCypher <a href=\"/products/epigenetics-reagents-and-assays/cutana-chic-cut-and-run-kit\" target=\"_blank\">14-1048</a>). Library preparation was performed with 5 ng of DNA (or the total amount\n recovered if less than 5 ng) using the CUTANA™ Library Prep Kit (EpiCypher <a href=\"/products/epigenetics-reagents-and-assays/cutana-cut-and-run-library-prep-kit\" target=\"_blank\">14-1001/14-1002</a>).\n Libraries were run on an Illumina NextSeq2000 with paired-end sequencing (2x50 bp). Sample\n sequencing depth (vector control cells/overexpressing cells) was 5.9/6.2 million reads (IgG),\n 13.7/12.6 million reads (H3K4me3), 8.1/5.2 million reads (GATA3), and 5.7/5.9 million reads (HA\n Tag). Data were aligned to the hg19 genome using Bowtie2. Data were filtered to remove duplicates,\n multi-aligned reads, and ENCODE DAC Exclusion List regions.\n </span>\n </p>\n </div>\n </div>\n <aside class=\"image-picker__right\">\n <div class=\"image-picker__gallery\">\n <img loading=\"lazy\" alt=\"13-2010-spike-ins-assay-control\"\n src=\"/content/images/products/antibodies/13-2010-spike-ins-assay-control.jpeg\" width=\"200\"\n class=\"image-picker__side-image image-picker__side-image_active\" role=\"button\" />\n <img loading=\"lazy\" alt=\"13-2010-ha-tagged-protein\"\n src=\"/content/images/products/antibodies/13-2010-ha-tagged-protein-thumb.jpeg\" class=\"image-picker__side-image\"\n role=\"button\" />\n <img loading=\"lazy\" alt=\"13-2010-browser-tracks\"\n src=\"/content/images/products/antibodies/13-2010-browser-tracks.jpeg\" class=\"image-picker__side-image\"\n role=\"button\" />\n <img loading=\"lazy\" alt=\"13-2010-western-blot\"\n src=\"/content/images/products/antibodies/13-2010-western-blot.jpeg\"\n class=\"image-picker__side-image\" role=\"button\" />\n <img loading=\"lazy\" alt=\"cut-and-run-methods\"\n src=\"https://cdn11.bigcommerce.com/s-y9o92/images/stencil/original/image-manager/2022.07.06-c-r-workflow-simple-rgb-ew.png?t=1697220982&_gl=1*u66xe1*_ga*NDc4MDE2MzAxLjE2OTY2MjA3NTU.*_ga_WS2VZYPC6G*MTY5NzY5NzkxNi4zNi4xLjE2OTc2OTk4OTEuNTYuMC4w\"\n class=\"image-picker__side-image\" role=\"button\" />\n </div>\n </aside>\n </section>\n </div>\n </div>\n </div>\n <div id=\"prodAccordion\">\n <div id=\"ProductDescription\" class=\"Block Panel\">\n <h3 class=\"sub-title1\">Technical Information</h3>\n <div class=\"ProductDescriptionContainer product-droppdown__section-description\">\n <div class=\"product-tech-info\">\n <div class=\"product-tech-info__line-item\">\n <div class=\"product-tech-info__line-item-left\">\n <b>Immunogen</b>\n </div>\n <div class=\"product-tech-info__line-item-right\">\n A synthetic HA peptide (sequence: YPYDVPDYA)\n </div>\n </div>\n <div class=\"product-tech-info__line-item\">\n <div class=\"product-tech-info__line-item-left\">\n <b>Storage</b>\n </div>\n <div class=\"product-tech-info__line-item-right\">\n Stable for 1 year at 4°C from date of receipt\n </div>\n </div>\n <div class=\"product-tech-info__line-item\">\n <div class=\"product-tech-info__line-item-left\">\n <b>Formulation</b>\n </div>\n <div class=\"product-tech-info__line-item-right\">\n Antigen affinity-purified antibody in phosphate buffered\n saline (PBS), 0.09% sodium azide\n </div>\n </div>\n </div>\n </div>\n </div>\n </div>\n <div id=\"prodAccordion\">\n <div id=\"ProductDescription\" class=\"Block Panel\">\n <h3 class=\"sub-title1\">Recommended Dilution</h3>\n <div class=\"ProductDescriptionContainer product-droppdown__section-description\">\n <div class=\"product-tech-info\">\n <div class=\"product-tech-info__line-item\">\n <div class=\"product-tech-info__line-item-left\">\n <b>CUT&RUN:</b>\n </div>\n <div class=\"product-tech-info__line-item-right\">\n 0.5 µg per reaction\n </div>\n </div>\n <div class=\"product-tech-info__line-item\">\n <div class=\"product-tech-info__line-item-left\">\n <b>Western Blot (WB):</b>\n </div>\n <div class=\"product-tech-info__line-item-right\">\n 1:1,000 - 1:30,000\n </div>\n </div>\n </div>\n </div>\n </div>\n </div>\n <div id=\"prodAccordion\">\n <div id=\"ProductDescription\" class=\"Block Panel\">\n <h3 class=\"sub-title1\">Documents & Resources</h3>\n <div class=\"ProductDescriptionContainer product-droppdown__section-description\">\n <div class=\"product-documents\">\n <a href=\"/content/documents/tds/13-2010.pdf\" target=\"_new\" class=\"product-documents__link\">\n <svg version=\"1.1\" id=\"Layer_1\" xmlns=\"http://www.w3.org/2000/svg\"\n xmlns:xlink=\"http://www.w3.org/1999/xlink\" x=\"0px\" y=\"0px\" viewBox=\"0 0 228 240\"\n style=\"enable-background: new 0 0 228 240\" xml:space=\"preserve\" class=\"product-documents__icon\">\n <g>\n <path class=\"product-documents__svg-pdf\"\n d=\"M191.92,68.77l-47.69-47.69c-1.33-1.33-3.12-2.08-5.01-2.08H45.09C41.17,19,38,22.17,38,26.09v184.36\n c0,3.92,3.17,7.09,7.09,7.09h141.82c3.92,0,7.09-3.17,7.09-7.09V73.8C194,71.92,193.25,70.1,191.92,68.77z M177.65,77.06h-41.7\n v-41.7L177.65,77.06z M178.05,201.59H53.95V34.95h66.92v47.86c0,5.14,4.17,9.31,9.31,9.31h47.86V201.59z\" />\n </g>\n <rect x=\"20\" y=\"112\" class=\"product-documents__svg-background\" width=\"146\" height=\"76\" />\n <g>\n <path class=\"product-documents__svg-pdf\" d=\"M23.83,125.68h22.36c5.29,0,9.41,1.33,12.35,4c2.94,2.67,4.42,6.39,4.42,11.18c0,4.78-1.47,8.51-4.42,11.18\n c-2.94,2.67-7.06,4-12.35,4H34.59v18.29H23.83V125.68z M44.81,147.9c5.38,0,8.07-2.32,8.07-6.97c0-2.39-0.67-4.16-2-5.31\n c-1.33-1.15-3.36-1.73-6.07-1.73H34.59v14.01H44.81z\" />\n <path class=\"product-documents__svg-pdf\"\n d=\"M69.92,125.68h18.91c5.29,0,9.84,0.97,13.66,2.9c3.82,1.93,6.74,4.72,8.76,8.35\n c2.02,3.63,3.04,7.98,3.04,13.04c0,5.06-1,9.42-3,13.08c-2,3.66-4.91,6.45-8.73,8.38c-3.82,1.93-8.4,2.9-13.73,2.9H69.92V125.68z\n M88.07,165.63c10.35,0,15.52-5.22,15.52-15.66c0-10.4-5.17-15.59-15.52-15.59h-7.38v31.26H88.07z\" />\n <path class=\"product-documents__svg-pdf\"\n d=\"M122.57,125.68h32.84v8.49h-22.22v11.18h20.84v8.49h-20.84v20.49h-10.63V125.68z\" />\n </g>\n </svg>\n <span class=\"product-documents__info\">Technical Datasheet</span>\n </a>\n </div>\n </div>\n </div>\n </div>\n <div id=\"prodAccordion\">\n <div id=\"ProductDescription\" class=\"Block Panel\">\n <h3 class=\"sub-title1\">Additional Info</h3>\n <div class=\"ProductDescriptionContainer product-droppdown__section-description\">\n <p>\n This product is provided for commercial sale under license\n from Bethyl Laboratories, Inc.\n </p>\n </div>\n </div>\n </div>\n <div id=\"prodAccordion\">\n <div id=\"ProductDescription\" class=\"Block Panel current\">\n <h3 id=\"bioz\" style=\"padding-bottom: 5px; margin-top: 1rem\" class=\"sub-title1\">\n Product References\n </h3>\n\n <div class=\"ProductDescriptionContainer product-droppdown__section-description-specific\">\n <object id=\"wobj-3835-13-2010-q\" type=\"text/html\" data=\"https://www.bioz.com/v_widget_6_0/3835/13-2010/\"\n style=\"width: 100%; height: 193px\"></object>\n <div id=\"bioz-w-pb-3835-13-2010-q-div\" style=\"width: 100%\">\n <a id=\"bioz-w-pb-3835-13-2010-q\" style=\"\n font-size: 12px;\n text-decoration: none;\n color: #4698cb;\n \" href=\"https://www.bioz.com/\" target=\"_blank\"><img src=\"https://cdn.bioz.com/assets/favicon.png\" style=\"\n width: 11px;\n height: 11px;\n vertical-align: baseline;\n padding-bottom: 0px;\n margin-left: 0px;\n margin-bottom: 0px;\n float: none;\n \" />\n Powered by Bioz</a>\n <a style=\"\n font-size: 12px;\n text-decoration: none;\n float: right;\n color: transparent;\n \" href=\"https://www.bioz.com/result/13-2010/product/EpiCypher/?cn=13-2010\" target=\"_blank\">\n See more details on Bioz</a>\n </div>\n </div>\n </div>\n </div>\n </div>\n</div>\n<script>\n $(document).ready(function () {\n var widget_micro_obj = new v_widget_obj(\"s\", [1]);\n widget_micro_obj.request_catalog_number_widget_data_internal(\n \"13-2010\",\n \"13-2010\"\n );\n });\n</script>\n<style>\n .bioz-w-header td {\n padding: 0;\n }\n\n td table {\n margin: 0;\n padding: 6px !important;\n }\n</style>","tags":[],"warranty":"","price":{"without_tax":{"formatted":"$495.00","value":495,"currency":"USD"},"tax_label":"Sales Tax"},"detail_messages":"","availability":"","page_title":"HA Tag Antibody | CUTANA™ CUT&RUN Compatible","cart_url":"https://www.epicypher.com/cart.php","max_purchase_quantity":0,"mpn":null,"upc":null,"options":[],"related_products":[{"id":772,"sku":"14-1048","name":"CUTANA™ ChIC/CUT&RUN Kit","url":"https://www.epicypher.com/products/epigenetics-reagents-and-assays/cutana-chic-cut-and-run-kit","availability":"","rating":null,"brand":{"name":null},"category":["Epigenetics Kits and 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antibody","show_quantity_input":1,"title":"HA Tag CUTANA™ CUT&RUN Antibody","gift_wrapping_available":false,"min_purchase_quantity":0,"customizations":[],"images":[{"data":"https://cdn11.bigcommerce.com/s-y9o92/images/stencil/{:size}/products/875/937/ha-tag-cutana-cutandrun-antibody__43540.1645734529.jpg?c=2","alt":"HA Tag CUTANA CUTandRUN Antibody"}]} Pack Size: 100 µg
- Type: Polyclonal
- Host: Rabbit
- Applications: CUT&RUN, WB
- Reactivity: HA Epitope (YPYDVPDYA)
- Format: Antigen affinity-purified
- Target Size: N/A
Description
This antibody meets EpiCypher’s "CUTANA Compatible" criteria for performance in Cleavage Under Targets and Release Using Nuclease (CUT&RUN) and/or Cleavage Under Targets and Tagmentation (CUT&Tag) approaches to genomic mapping. Every lot of a CUTANA Compatible antibody is tested in the indicated approach using EpiCypher optimized protocols and determined to yield peaks that show a genomic distribution pattern consistent with reported function(s) of the target. HA antibody is useful for studies utilizing HA-tagged target proteins. HA Tag antibody produces CUT&RUN peaks (Figure 2) in breast cancer cells expressing 3xHA-tagged GATA3 transcription factor [Takaku et al., 2016]*.
Pair with our SNAP-CUTANA™ HA Tag Panel for CUT&RUN success. Check it out here.
*Thanks to Dr. Takaku (UND) for 3xFlag-GATA3-3xHA MDA-MB-231 cells.Validation Data
Figure 1: Defined nucleosome spike-ins provide
in-assay control for HA Tag antibody in CUT&RUN
CUT&RUN sequencing reads were aligned to the unique DNA barcodes corresponding to each nucleosome in
the SNAP-CUTANA™ HA Tag Panel (EpiCypher 19-5002). Data are expressed as a percent relative to
on-target recovery (HA Tag set to 100%) or total counts (IgG/GATA3). IgG/GATA3 show no preferential
binding to unmodified or HA spike-in nucleosomes. HA Tag antibody selectively enriches the HA Tag
spike-in nucleosome, validating the antibody in CUT&RUN.
Figure 2: HA-tagged protein peaks in
CUT&RUN
Heatmaps show HA Tag antibody-enriched peaks called for GATA3-3xHA overexpressing cells (OE) in
aligned rows relative to all other experimental conditions. Rows are ranked by intensity (top to
bottom) and colored such that red indicates high localized enrichment and blue denotes background
signal. A high degree of overlap is observed for HA and GATA3 antibodies as expected in the OE
cells, while empty vector control (Ctrl) shows absence of HA and lower GATA3 enrichment representing
endogenous protein. IgG shows low background signal. H3K4me3, a canonical mark of promoters, does
not appear in regions of high GATA3 enrichment.
Figure 3: CUT&RUN representative browser tracks for
HA-tagged protein
Gene browser shows were generated using the Integrative Genomics Viewer (IGV, Broad Institute). Two
of the top called peaks in GATA3-3xHA overexpressing (OE) cells are shown. The peaks show the same
distribution patterns as observed in the genome-wide heatmaps (Figure 2). IgG shows low background,
H3K4me3 is unchanged between empty vector control (Ctrl) and OE cells, GATA3 peaks are more robust
in OE cells, and peaks overlap between GATA3 and HA antibodies in OE cells. These results
demonstrate the robustness and specificity of HA Tag antibody in CUT&RUN experiments targeting
HA-tagged proteins.
Figure 4: Western blot data
E. coli cells expressing a multi-tag fusion protein were used to prepare whole cell lysates. The
indicated amounts (ng) of lysate were loaded onto a 4-20% SDS-PAGE gel and analyzed under standard
western blot conditions using HA Tag antibody at a dilution of 1:25,000.
Figure 5: CUT&RUN Methods
CUT&RUN was performed on 500k MDA-MB-231 native cells stably overexpressing c-terminal 3xHA-tagged
GATA3 [1] or containing vector control. 0.5 µg of either HA Tag, H3K4me3 (EpiCypher 13-0060), GATA3
(CST 5852), or IgG (EpiCypher 13-0042) antibodies were used with the CUTANA™ ChIC/CUT&RUN Kit v4
(EpiCypher 14-1048). Library preparation was performed with 5 ng of DNA (or the total amount
recovered if less than 5 ng) using the CUTANA™ Library Prep Kit (EpiCypher 14-1001/14-1002).
Libraries were run on an Illumina NextSeq2000 with paired-end sequencing (2x50 bp). Sample
sequencing depth (vector control cells/overexpressing cells) was 5.9/6.2 million reads (IgG),
13.7/12.6 million reads (H3K4me3), 8.1/5.2 million reads (GATA3), and 5.7/5.9 million reads (HA
Tag). Data were aligned to the hg19 genome using Bowtie2. Data were filtered to remove duplicates,
multi-aligned reads, and ENCODE DAC Exclusion List regions.
Technical Information
Recommended Dilution
Documents & Resources
Additional Info
This product is provided for commercial sale under license from Bethyl Laboratories, Inc.