JUN/c-Jun CUTANA™ CUT&RUN Antibody
{"url":"https://www.epicypher.com/products/antibodies/cutana-cut-run-antibodies/jun-c-jun-cutana-cut-run-antibody","add_this":[{"service":"facebook","annotation":""},{"service":"email","annotation":""},{"service":"print","annotation":""},{"service":"twitter","annotation":""},{"service":"linkedin","annotation":""}],"gtin":null,"id":"918","bulk_discount_rates":[],"can_purchase":true,"meta_description":"Rabbit polyclonal JUN/c-Jun antibody rigorously tested for robust and reliable performance in CUT&RUN assays. Also validated for IHC, IP, & WB. ","category":["Antibodies/CUTANA™ CUT&RUN Antibodies","Antibodies/CUTANA™ CUT&RUN Antibodies/CUT&RUN Antibodies - Chromatin-Associated Proteins","Epigenetics Kits and Reagents/CUTANA™ ChIC / CUT&RUN Assays"],"AddThisServiceButtonMeta":"","main_image":{"data":"https://cdn11.bigcommerce.com/s-y9o92/images/stencil/{:size}/products/918/1019/chd3-cutana-cutandrun-antibody__66975.1645734525__91145.1648666822__03424.1651862137__58623.1652127357__88640.1652155700__67172.1652156235.jpg?c=2","alt":"JUN/c-Jun CUTANA™ CUT&RUN Antibody"},"add_to_wishlist_url":"/wishlist.php?action=add&product_id=918","shipping":{"calculated":true},"num_reviews":0,"weight":"0.01 LBS","custom_fields":[{"id":"1086","name":"Pack Size","value":"100 µL"}],"sku":"13-2019","description":"<div class=\"product-general-info\">\n <ul class=\"product-general-info__list-left\">\n <li class=\"product-general-info__list-item\">\n <strong>Type: </strong>Polyclonal\n </li>\n <li class=\"product-general-info__list-item\">\n <strong>Host: </strong>Rabbit\n </li>\n <li class=\"product-general-info__list-item\">\n <strong>Applications: </strong>CUT&RUN, IHC, IP, WB\n </li>\n </ul>\n <ul class=\"product-general-info__list-right\">\n <li class=\"product-general-info__list-item\">\n <strong>Reactivity: </strong>Human, Mouse\n </li>\n <li class=\"product-general-info__list-item\">\n <strong>Format: </strong>Antigen affinity-purified\n </li>\n <li class=\"product-general-info__list-item\">\n <strong>Target Size: </strong>35 kDa\n </li>\n </ul>\n</div>\n\n<div class=\"service_accordion product-droppdown\">\n <div class=\"container\">\n <div id=\"prodAccordion\">\n <div id=\"ProductDescription\" class=\"Block Panel current\">\n <h3 class=\"sub-title1\">Description</h3>\n <div\n class=\"ProductDescriptionContainer product-droppdown__section-description-specific\">\n <p>\n This antibody meets EpiCypher’s \"CUTANA Compatible\" criteria for\n performance in Cleavage Under Targets and Release Using Nuclease\n (CUT&RUN) and/or Cleavage Under Targets and Tagmentation (CUT&Tag)\n approaches to genomic mapping. Every lot of a CUTANA Compatible\n antibody is tested in the indicated approach using EpiCypher\n optimized <a href=\"/resources/protocols/\">protocols</a> and\n determined to yield peaks that show a genomic distribution pattern\n consistent with reported function(s) of the target protein.\n JUN/c-Jun heterodimerizes with proteins of the FOS family to form an\n AP-1 transcription complex and binds to the AP-1 consensus motif\n [1]. JUN/c-Jun antibody produces CUT&RUN peaks above background\n primarily in intronic, intergenic, and promoter regions (<strong\n >Figure 1</strong\n >) that overlap with known JUN/c-Jun DNA-binding motifs (<strong\n >Figure 2</strong\n >).\n </p>\n </div>\n </div>\n </div>\n <div id=\"prodAccordion\">\n <div id=\"ProductDescription\" class=\"Block Panel current\">\n <h3 class=\"sub-title1\">Validation Data</h3>\n <div\n class=\"ProductDescriptionContainer product-droppdown__section-description-specific\">\n <p>\n CUT&RUN was performed on 500k HeLa cells with 0.5 µg of either\n JUN/c-Jun or IgG negative control (EpiCypher\n <a\n href=\"/products/nucleosomes/snap-cutana-spike-in-controls/cutana-rabbit-igg-cut-run-negative-control-antibody\"\n >13-0042</a\n >) antibodies using the CUTANA™ ChIC/CUT&RUN Kit v2.0 (EpiCypher\n <a\n href=\"/products/epigenetics-reagents-and-assays/cutana-chic-cut-and-run-kit\"\n >14-1048</a\n >). Library preparation was performed with 5 ng of DNA (or the total\n amount recovered if less than 5 ng) using the CUTANA™ CUT&RUN\n Library Prep Kit (EpiCypher\n <a\n href=\"/products/epigenetics-reagents-and-assays/cutana-cut-and-run-library-prep-kit\"\n >14-1001/14-1002</a\n >). Both kit protocols were adapted for high throughput Tecan liquid\n handling. Libraries were run on an Illumina NextSeq2000 with\n paired-end sequencing (2x50 bp). Sample sequencing depth was 5.1\n million reads (IgG), 12.1 million reads (JUN/c-Jun), and 6.2 million\n reads (H3K4me3). Data were aligned to the hg19 genome using Bowtie2.\n Data were filtered to remove duplicates, multi-aligned reads, and\n ENCODE DAC Exclusion List regions.\n </p>\n\n <section class=\"image-picker\">\n <div class=\"image-picker__left\">\n <div\n class=\"image-picker__main-content_active image-picker__main-content\">\n <div class=\"image-picker__header-content\">\n <button class=\"image-picker__left-arrow\">\n <svg\n class=\"image-picker__svg-left\"\n width=\"24\"\n height=\"24\"\n viewBox=\"0 0 24 24\">\n <path\n d=\"M16.67 0l2.83 2.829-9.339 9.175 9.339 9.167-2.83 2.829-12.17-11.996z\" />\n </svg>\n </button>\n <a\n href=\"/content/images/products/antibodies/13-2019-JUNc-Jun-peaks.jpeg\"\n target=\"_blank\"\n class=\"image-picker__main-image-link\"\n ><img loading=\"lazy\"\n alt=\"13-2019-JUNc-Jun-peaks\"\n src=\"/content/images/products/antibodies/13-2019-JUNc-Jun-peaks.jpeg\"\n class=\"image-picker__main-image\" />\n <span class=\"image-picker__main-image-caption\"\n >(Click to enlarge)</span\n ></a\n >\n <button class=\"image-picker__right-arrow\">\n <svg\n class=\"image-picker__svg-right\"\n width=\"24\"\n height=\"24\"\n viewBox=\"0 0 24 24\">\n <path\n d=\"M7.33 24l-2.83-2.829 9.339-9.175-9.339-9.167 2.83-2.829 12.17 11.996z\" />\n </svg>\n </button>\n </div>\n <p>\n <span class=\"image-picker__span-content\"\n ><strong>Figure 1: JUN/c-Jun peaks in CUT&RUN </strong\n ><br />\n CUT&RUN was performed as described above. Peaks were called\n with MACS2. Heatmaps show JUN/c-Jun peaks relative to IgG\n negative control antibody in aligned rows ranked by\n intensity (top to bottom) and colored such that red\n indicates high localized enrichment and blue denotes\n background signal (<strong>left</strong>). The number of\n peaks that fall into distinct classes of functionally\n annotated genomic regions are shown\n (<strong>right</strong>).\n </span>\n </p>\n </div>\n <div class=\"image-picker__main-content\">\n <div class=\"image-picker__header-content\">\n <button class=\"image-picker__left-arrow\">\n <svg\n class=\"image-picker__svg-left\"\n width=\"24\"\n height=\"24\"\n viewBox=\"0 0 24 24\">\n <path\n d=\"M16.67 0l2.83 2.829-9.339 9.175 9.339 9.167-2.83 2.829-12.17-11.996z\" />\n </svg>\n </button>\n <a\n href=\"/content/images/products/antibodies/13-2019-binding-motif-analysis.jpeg\"\n target=\"_blank\"\n class=\"image-picker__main-image-link\"\n ><img loading=\"lazy\"\n alt=\"13-2019-binding-motif-analysis\"\n src=\"/content/images/products/antibodies/13-2019-binding-motif-analysis.jpeg\"\n class=\"image-picker__main-image\" />\n <span class=\"image-picker__main-image-caption\"\n >(Click to enlarge)</span\n ></a\n >\n <button class=\"image-picker__right-arrow\">\n <svg\n class=\"image-picker__svg-right\"\n width=\"24\"\n height=\"24\"\n viewBox=\"0 0 24 24\">\n <path\n d=\"M7.33 24l-2.83-2.829 9.339-9.175-9.339-9.167 2.83-2.829 12.17 11.996z\" />\n </svg>\n </button>\n </div>\n <p>\n <span class=\"image-picker__span-content\"\n ><strong\n >Figure 2: JUN/c-Jun transcription factor binding motif\n analysis in CUT&RUN</strong\n ><br />\n Fra1(bZIP) /BT549-Fra1-ChIP-Seq(GSE46166)/Homer consensus\n motif, represented as a sequence logo position weight\n matrix, was the top called motif significantly enriched\n under JUN/c-Jun CUT&RUN peaks (<strong>left</strong>). The\n number of JUN/c-Jun peaks containing the consensus motif\n from the left panel is represented by a Venn Diagram\n (<strong>right</strong>).\n </span>\n </p>\n </div>\n <div class=\"image-picker__main-content\">\n <div class=\"image-picker__header-content\">\n <button class=\"image-picker__left-arrow\">\n <svg\n class=\"image-picker__svg-left\"\n width=\"24\"\n height=\"24\"\n viewBox=\"0 0 24 24\">\n <path\n d=\"M16.67 0l2.83 2.829-9.339 9.175 9.339 9.167-2.83 2.829-12.17-11.996z\" />\n </svg>\n </button>\n <a\n href=\"/content/images/products/antibodies/13-2019-representative-browser-tracks.jpeg\"\n target=\"_blank\"\n class=\"image-picker__main-image-link\"\n ><img loading=\"lazy\"\n alt=\"13-2019-representative-browser-tracks\"\n src=\"/content/images/products/antibodies/13-2019-representative-browser-tracks.jpeg\"\n class=\"image-picker__main-image\" />\n <span class=\"image-picker__main-image-caption\"\n >(Click to enlarge)</span\n ></a\n >\n <button class=\"image-picker__right-arrow\">\n <svg\n class=\"image-picker__svg-right\"\n width=\"24\"\n height=\"24\"\n viewBox=\"0 0 24 24\">\n <path\n d=\"M7.33 24l-2.83-2.829 9.339-9.175-9.339-9.167 2.83-2.829 12.17 11.996z\" />\n </svg>\n </button>\n </div>\n <p>\n <span class=\"image-picker__span-content\"\n ><strong\n >Figure 3: JUN/c-Jun CUT&RUN representative browser\n tracks</strong\n ><br />\n CUT&RUN was performed as described above. Gene browser shots\n were generated using the Integrative Genomics Viewer (IGV,\n Broad Institute). Three representative loci of the top\n called peaks are shown.\n </span>\n </p>\n </div>\n <div class=\"image-picker__main-content\">\n <div class=\"image-picker__header-content\">\n <button class=\"image-picker__left-arrow\">\n <svg\n class=\"image-picker__svg-left\"\n width=\"24\"\n height=\"24\"\n viewBox=\"0 0 24 24\">\n <path\n d=\"M16.67 0l2.83 2.829-9.339 9.175 9.339 9.167-2.83 2.829-12.17-11.996z\" />\n </svg>\n </button>\n <a\n href=\"/content/images/products/antibodies/13-2019-immunohistochemistry-data.jpeg\"\n target=\"_blank\"\n class=\"image-picker__main-image-link\"\n ><img loading=\"lazy\"\n alt=\"13-2019-immunohistochemistry-data\"\n src=\"/content/images/products/antibodies/13-2019-immunohistochemistry-data.jpeg\"\n class=\"image-picker__main-image\" />\n <span class=\"image-picker__main-image-caption\"\n >(Click to enlarge)</span\n ></a\n >\n <button class=\"image-picker__right-arrow\">\n <svg\n class=\"image-picker__svg-right\"\n width=\"24\"\n height=\"24\"\n viewBox=\"0 0 24 24\">\n <path\n d=\"M7.33 24l-2.83-2.829 9.339-9.175-9.339-9.167 2.83-2.829 12.17 11.996z\" />\n </svg>\n </button>\n </div>\n <p>\n <span class=\"image-picker__span-content\"\n ><strong>Figure 4: Immunohistochemistry data </strong><br />\n FFPE section of human breast carcinoma using JUN/c-Jun\n antibody at a dilution of 1:200.\n </span>\n </p>\n </div>\n <div class=\"image-picker__main-content\">\n <div class=\"image-picker__header-content\">\n <button class=\"image-picker__left-arrow\">\n <svg\n class=\"image-picker__svg-left\"\n width=\"24\"\n height=\"24\"\n viewBox=\"0 0 24 24\">\n <path\n d=\"M16.67 0l2.83 2.829-9.339 9.175 9.339 9.167-2.83 2.829-12.17-11.996z\" />\n </svg>\n </button>\n <a\n href=\"/content/images/products/antibodies/13-2019-immunoprecipitation-data.jpeg\"\n target=\"_blank\"\n class=\"image-picker__main-image-link\"\n ><img loading=\"lazy\"\n alt=\"13-2019-immunoprecipitation-data\"\n src=\"/content/images/products/antibodies/13-2019-immunoprecipitation-data.jpeg\"\n class=\"image-picker__main-image\" />\n <span class=\"image-picker__main-image-caption\"\n >(Click to enlarge)</span\n ></a\n >\n <button class=\"image-picker__right-arrow\">\n <svg\n class=\"image-picker__svg-right\"\n width=\"24\"\n height=\"24\"\n viewBox=\"0 0 24 24\">\n <path\n d=\"M7.33 24l-2.83-2.829 9.339-9.175-9.339-9.167 2.83-2.829 12.17 11.996z\" />\n </svg>\n </button>\n </div>\n <p>\n <span class=\"image-picker__span-content\"\n ><strong>Figure 5: Immunoprecipitation data </strong><br />\n EpiCypher JUN/c-Jun antibody (3 µg/mg lysate) was used to\n immunoprecipitate whole cell lysates (1 mg, 20% of IP\n loaded) isolated from HEK293T cells. A negative control IgG\n antibody and positive control antibody targeting a different\n JUN/c-Jun epitope (Bethyl Laboratories) were also used to\n demonstrate the specificity of the IP. For blotting\n immunoprecipitates, EpiCypher JUN/c-Jun antibody was used at\n a dilution of 1:200.\n </span>\n </p>\n </div>\n <div class=\"image-picker__main-content\">\n <div class=\"image-picker__header-content\">\n <button class=\"image-picker__left-arrow\">\n <svg\n class=\"image-picker__svg-left\"\n width=\"24\"\n height=\"24\"\n viewBox=\"0 0 24 24\">\n <path\n d=\"M16.67 0l2.83 2.829-9.339 9.175 9.339 9.167-2.83 2.829-12.17-11.996z\" />\n </svg>\n </button>\n <a\n href=\"/content/images/products/antibodies/13-2019-western-blot-data.jpeg\"\n target=\"_blank\"\n class=\"image-picker__main-image-link\"\n ><img loading=\"lazy\"\n alt=\"13-2019-western-blot-data\"\n src=\"/content/images/products/antibodies/13-2019-western-blot-data.jpeg\"\n class=\"image-picker__main-image\" />\n <span class=\"image-picker__main-image-caption\"\n >(Click to enlarge)</span\n ></a\n >\n <button class=\"image-picker__right-arrow\">\n <svg\n class=\"image-picker__svg-right\"\n width=\"24\"\n height=\"24\"\n viewBox=\"0 0 24 24\">\n <path\n d=\"M7.33 24l-2.83-2.829 9.339-9.175-9.339-9.167 2.83-2.829 12.17 11.996z\" />\n </svg>\n </button>\n </div>\n <p>\n <span class=\"image-picker__span-content\"\n ><strong>Figure 6: Western blot data </strong><br />\n Western analysis of JUN/c-Jun in whole cell extracts from\n HEK293T (5, 15, and 50 µg), HeLa (50 µg), and mouse NIH 3T3\n (50 µg) cells. Lysates were resolved via SDS-PAGE and\n detected with a 1:5,000 dilution of JUN/c-Jun antibody.\n </span>\n </p>\n </div>\n </div>\n <aside class=\"image-picker__right\">\n <div class=\"image-picker__gallery\">\n <img loading=\"lazy\"\n alt=\"13-2019-JUNc-Jun-peaks\"\n src=\"/content/images/products/antibodies/13-2019-JUNc-Jun-peaks.jpeg\"\n width=\"200\"\n class=\"image-picker__side-image image-picker__side-image_active\"\n role=\"button\" />\n <img loading=\"lazy\"\n alt=\"13-2019-binding-motif-analysis\"\n src=\"/content/images/products/antibodies/13-2019-binding-motif-analysis.jpeg\"\n width=\"200\"\n class=\"image-picker__side-image image-picker__side-image_active\"\n role=\"button\" />\n <img loading=\"lazy\"\n alt=\"13-2019-representative-browser-tracks\"\n src=\"/content/images/products/antibodies/13-2019-representative-browser-tracks.jpeg\"\n width=\"200\"\n class=\"image-picker__side-image image-picker__side-image_active\"\n role=\"button\" />\n <img loading=\"lazy\"\n alt=\"13-2019-immunohistochemistry-data\"\n src=\"/content/images/products/antibodies/13-2019-immunohistochemistry-data.jpeg\"\n width=\"200\"\n class=\"image-picker__side-image image-picker__side-image_active\"\n role=\"button\" />\n <img loading=\"lazy\"\n alt=\"13-2019-immunoprecipitation-data\"\n src=\"/content/images/products/antibodies/13-2019-immunoprecipitation-data.jpeg\"\n width=\"200\"\n class=\"image-picker__side-image image-picker__side-image_active\"\n role=\"button\" />\n <img loading=\"lazy\"\n alt=\"13-2019-western-blot-data\"\n src=\"/content/images/products/antibodies/13-2019-western-blot-data.jpeg\"\n width=\"200\"\n class=\"image-picker__side-image image-picker__side-image_active\"\n role=\"button\" />\n </div>\n </aside>\n </section>\n </div>\n </div>\n </div>\n <div id=\"prodAccordion\">\n <div id=\"ProductDescription\" class=\"Block Panel\">\n <h3 class=\"sub-title1\">Technical Information</h3>\n <div\n class=\"ProductDescriptionContainer product-droppdown__section-description\">\n <div class=\"product-tech-info\">\n <div class=\"product-tech-info__line-item\">\n <div class=\"product-tech-info__line-item-left\">\n <b>Immunogen</b>\n </div>\n <div class=\"product-tech-info__line-item-right\">\n Between amino acids 1 and 40\n </div>\n </div>\n <div class=\"product-tech-info__line-item\">\n <div class=\"product-tech-info__line-item-left\">\n <b>Storage</b>\n </div>\n <div class=\"product-tech-info__line-item-right\">\n Stable for 1 year at 4°C from date of receipt\n </div>\n </div>\n <div class=\"product-tech-info__line-item\">\n <div class=\"product-tech-info__line-item-left\">\n <b>Formulation</b>\n </div>\n <div class=\"product-tech-info__line-item-right\">\n Antigen affinity-purified antibody in Tris-buffered saline, 0.1%\n BSA, 0.09% sodium azide\n </div>\n </div>\n </div>\n </div>\n </div>\n </div>\n <div id=\"prodAccordion\">\n <div id=\"ProductDescription\" class=\"Block Panel\">\n <h3 class=\"sub-title1\">Recommended Dilution</h3>\n <div\n class=\"ProductDescriptionContainer product-droppdown__section-description\">\n <!-- <p><strong>CUT&RUN:</strong> 0.5 µg per reaction</p>\n <p><strong>Immunoprecipitation (IP):</strong> 2-10 µg/mg lysate</p>\n <p><strong>Western Blot (WB):</strong> 1:10,000 - 1:25,000</p> -->\n\n <div class=\"product-tech-info\">\n <div class=\"product-tech-info__line-item\">\n <div class=\"product-tech-info__line-item-left\">\n <b>CUT&RUN:</b>\n </div>\n <div class=\"product-tech-info__line-item-right\">\n 0.5 µg per reaction\n </div>\n </div>\n <div class=\"product-tech-info__line-item\">\n <div class=\"product-tech-info__line-item-left\">\n <b>Immunohistochemistry (IHC):</b>\n </div>\n <div class=\"product-tech-info__line-item-right\">\n 1:100 - 1:500. Epitope retrieval with citrate buffer pH 6.0 is\n recommended for FFPE tissue sections\n </div>\n </div>\n <div class=\"product-tech-info__line-item\">\n <div class=\"product-tech-info__line-item-left\">\n <b>Immunoprecipitation (IP):</b>\n </div>\n <div class=\"product-tech-info__line-item-right\">\n 2 - 5 µg/mg lysate\n </div>\n </div>\n <div class=\"product-tech-info__line-item\">\n <div class=\"product-tech-info__line-item-left\">\n <b>Western Blot (WB):</b>\n </div>\n <div class=\"product-tech-info__line-item-right\">\n 1:2,000 - 1:10,000\n </div>\n </div>\n </div>\n </div>\n </div>\n </div>\n <div id=\"prodAccordion\">\n <div id=\"ProductDescription\" class=\"Block Panel\">\n <h3 class=\"sub-title1\">Gene & Protein Information</h3>\n <div\n class=\"ProductDescriptionContainer product-droppdown__section-description\">\n <div class=\"product-tech-info\">\n <div class=\"product-tech-info__line-item\">\n <div class=\"product-tech-info__line-item-left\">\n <b>UniProt ID</b>\n </div>\n <div class=\"product-tech-info__line-item-right\">P05412</div>\n </div>\n <div class=\"product-tech-info__line-item\">\n <div class=\"product-tech-info__line-item-left\">\n <b>Gene Name</b>\n </div>\n <div class=\"product-tech-info__line-item-right\">JUN</div>\n </div>\n <div class=\"product-tech-info__line-item\">\n <div class=\"product-tech-info__line-item-left\">\n <b>Protein Name</b>\n </div>\n <div class=\"product-tech-info__line-item-right\">\n Transcription factor Jun\n </div>\n </div>\n <div class=\"product-tech-info__line-item\">\n <div class=\"product-tech-info__line-item-left\">\n <b>Target Size</b>\n </div>\n <div class=\"product-tech-info__line-item-right\">35 kDa</div>\n </div>\n <div class=\"product-tech-info__line-item\">\n <div class=\"product-tech-info__line-item-left\">\n <b>Alternate Names</b>\n </div>\n <div class=\"product-tech-info__line-item-right\">\n transcription factor Jun, activator protein 1, AP1,\n proto-oncogene c-Jun, transcription factor AP-1 subunit Jun,\n v-jun avian sarcoma virus 17 oncogene homolog, p39, AP-1, c-Jun,\n enhancer-binding protein AP1, Jun activation domain binding\n protein, jun oncogene\n </div>\n </div>\n </div>\n </div>\n </div>\n </div>\n <div id=\"prodAccordion\">\n <div id=\"ProductDescription\" class=\"Block Panel\">\n <h3 class=\"sub-title1\">References</h3>\n <div\n class=\"ProductDescriptionContainer product-droppdown__section-description\">\n <strong>Background References:</strong>\n <br />\n [1] van Dam & Castellazzi <em>Oncogene</em> (2001). PMID:\n <a\n href=\"https://pubmed.ncbi.nlm.nih.gov/11402340/\"\n title=\"Distinct roles of Jun : Fos and Jun : ATF dimers in oncogenesis\"\n target=\"new\">\n 11402340</a\n ><br />\n </div>\n </div>\n </div>\n <div id=\"prodAccordion\">\n <div id=\"ProductDescription\" class=\"Block Panel\">\n <h3 class=\"sub-title1\">Documents & Resources</h3>\n <div\n class=\"ProductDescriptionContainer product-droppdown__section-description\">\n <div class=\"product-documents\">\n <a\n href=\"/content/documents/tds/13-2019.pdf\"\n target=\"_blank\"\n class=\"product-documents__link\">\n <svg\n version=\"1.1\"\n id=\"Layer_1\"\n xmlns=\"http://www.w3.org/2000/svg\"\n xmlns:xlink=\"http://www.w3.org/1999/xlink\"\n x=\"0px\"\n y=\"0px\"\n viewBox=\"0 0 228 240\"\n style=\"enable-background: new 0 0 228 240\"\n xml:space=\"preserve\"\n class=\"product-documents__icon\"\n alt=\"16-0030 Datasheet\">\n <g>\n <path\n class=\"product-documents__svg-pdf\"\n 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c2.02,3.63,3.04,7.98,3.04,13.04c0,5.06-1,9.42-3,13.08c-2,3.66-4.91,6.45-8.73,8.38c-3.82,1.93-8.4,2.9-13.73,2.9H69.92V125.68z\n M88.07,165.63c10.35,0,15.52-5.22,15.52-15.66c0-10.4-5.17-15.59-15.52-15.59h-7.38v31.26H88.07z\" />\n <path\n class=\"product-documents__svg-pdf\"\n d=\"M122.57,125.68h32.84v8.49h-22.22v11.18h20.84v8.49h-20.84v20.49h-10.63V125.68z\" />\n </g>\n </svg>\n <span class=\"product-documents__info\">Technical Datasheet</span>\n </a>\n </div>\n </div>\n </div>\n </div>\n <div id=\"prodAccordion\">\n <div id=\"ProductDescription\" class=\"Block Panel\">\n <h3 class=\"sub-title1\">Additional Info</h3>\n <div\n class=\"ProductDescriptionContainer product-droppdown__section-description\">\n <p>\n This antibody is provided for commercial sale under license from\n Bethyl Laboratories, Inc.\n </p>\n </div>\n </div>\n </div>\n </div>\n</div>\n","tags":[],"warranty":"","price":{"without_tax":{"formatted":"$495.00","value":495,"currency":"USD"},"tax_label":"Sales 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","image":{"data":"https://cdn11.bigcommerce.com/s-y9o92/images/stencil/{:size}/products/889/997/Epicypher_CUTANA_CUTRUN_LibraryPrep_Box__64431.1646406521.png?c=2","alt":"CUTANA™ CUT&RUN Library Prep Kit"},"images":[{"data":"https://cdn11.bigcommerce.com/s-y9o92/images/stencil/{:size}/products/889/997/Epicypher_CUTANA_CUTRUN_LibraryPrep_Box__64431.1646406521.png?c=2","alt":"CUTANA™ CUT&RUN Library Prep Kit"}],"date_added":"31st Jan 2022","pre_order":false,"show_cart_action":true,"has_options":true,"stock_level":null,"low_stock_level":null,"qty_in_cart":0,"custom_fields":[{"id":983,"name":"Pack Size","value":"48 Reactions"}],"num_reviews":null,"weight":{"formatted":"0.01 LBS","value":0.01},"demo":false,"price":{"without_tax":{"currency":"USD","formatted":"$1,525.00","value":1525},"tax_label":"Sales Tax"},"add_to_wishlist_url":"/wishlist.php?action=add&product_id=889"},{"id":694,"sku":null,"name":"CUTANA™ pAG-MNase for ChIC/CUT&RUN 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Comment","value":"Excess in bottom of Venom"},{"id":1175,"name":"Internal Comment","value":"Bulk in Psylocke"}],"num_reviews":null,"weight":{"formatted":"0.01 LBS","value":0.01},"demo":false,"price":{"without_tax":{"currency":"USD","formatted":"$335.00","value":335},"price_range":{"min":{"without_tax":{"currency":"USD","formatted":"$335.00","value":335},"tax_label":"Sales Tax"},"max":{"without_tax":{"currency":"USD","formatted":"$1,295.00","value":1295},"tax_label":"Sales Tax"}},"tax_label":"Sales Tax"},"add_to_wishlist_url":"/wishlist.php?action=add&product_id=694"},{"id":758,"sku":"13-0042","name":"CUTANA™ IgG Negative Control Antibody for CUT&RUN and CUT&Tag","url":"https://www.epicypher.com/products/nucleosomes/snap-cutana-spike-in-controls/cutana-igg-negative-control-antibody-for-cut-run-and-cut-tag","availability":"","rating":null,"brand":{"name":null},"category":["Nucleosomes/SNAP-CUTANA™ Spike-in Controls","Antibodies/CUTANA™ CUT&RUN Antibodies","Epigenetics Kits and 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Antibody","gift_wrapping_available":false,"min_purchase_quantity":0,"customizations":[],"images":[{"data":"https://cdn11.bigcommerce.com/s-y9o92/images/stencil/{:size}/products/918/1019/chd3-cutana-cutandrun-antibody__66975.1645734525__91145.1648666822__03424.1651862137__58623.1652127357__88640.1652155700__67172.1652156235.jpg?c=2","alt":"JUN/c-Jun CUTANA™ CUT&RUN Antibody"}]} Pack Size: 100 µL
- Type: Polyclonal
- Host: Rabbit
- Applications: CUT&RUN, IHC, IP, WB
- Reactivity: Human, Mouse
- Format: Antigen affinity-purified
- Target Size: 35 kDa
Description
This antibody meets EpiCypher’s "CUTANA Compatible" criteria for performance in Cleavage Under Targets and Release Using Nuclease (CUT&RUN) and/or Cleavage Under Targets and Tagmentation (CUT&Tag) approaches to genomic mapping. Every lot of a CUTANA Compatible antibody is tested in the indicated approach using EpiCypher optimized protocols and determined to yield peaks that show a genomic distribution pattern consistent with reported function(s) of the target protein. JUN/c-Jun heterodimerizes with proteins of the FOS family to form an AP-1 transcription complex and binds to the AP-1 consensus motif [1]. JUN/c-Jun antibody produces CUT&RUN peaks above background primarily in intronic, intergenic, and promoter regions (Figure 1) that overlap with known JUN/c-Jun DNA-binding motifs (Figure 2).
Validation Data
CUT&RUN was performed on 500k HeLa cells with 0.5 µg of either JUN/c-Jun or IgG negative control (EpiCypher 13-0042) antibodies using the CUTANA™ ChIC/CUT&RUN Kit v2.0 (EpiCypher 14-1048). Library preparation was performed with 5 ng of DNA (or the total amount recovered if less than 5 ng) using the CUTANA™ CUT&RUN Library Prep Kit (EpiCypher 14-1001/14-1002). Both kit protocols were adapted for high throughput Tecan liquid handling. Libraries were run on an Illumina NextSeq2000 with paired-end sequencing (2x50 bp). Sample sequencing depth was 5.1 million reads (IgG), 12.1 million reads (JUN/c-Jun), and 6.2 million reads (H3K4me3). Data were aligned to the hg19 genome using Bowtie2. Data were filtered to remove duplicates, multi-aligned reads, and ENCODE DAC Exclusion List regions.
Figure 1: JUN/c-Jun peaks in CUT&RUN
CUT&RUN was performed as described above. Peaks were called
with MACS2. Heatmaps show JUN/c-Jun peaks relative to IgG
negative control antibody in aligned rows ranked by
intensity (top to bottom) and colored such that red
indicates high localized enrichment and blue denotes
background signal (left). The number of
peaks that fall into distinct classes of functionally
annotated genomic regions are shown
(right).
Figure 2: JUN/c-Jun transcription factor binding motif
analysis in CUT&RUN
Fra1(bZIP) /BT549-Fra1-ChIP-Seq(GSE46166)/Homer consensus
motif, represented as a sequence logo position weight
matrix, was the top called motif significantly enriched
under JUN/c-Jun CUT&RUN peaks (left). The
number of JUN/c-Jun peaks containing the consensus motif
from the left panel is represented by a Venn Diagram
(right).
Figure 3: JUN/c-Jun CUT&RUN representative browser
tracks
CUT&RUN was performed as described above. Gene browser shots
were generated using the Integrative Genomics Viewer (IGV,
Broad Institute). Three representative loci of the top
called peaks are shown.
Figure 4: Immunohistochemistry data
FFPE section of human breast carcinoma using JUN/c-Jun
antibody at a dilution of 1:200.
Figure 5: Immunoprecipitation data
EpiCypher JUN/c-Jun antibody (3 µg/mg lysate) was used to
immunoprecipitate whole cell lysates (1 mg, 20% of IP
loaded) isolated from HEK293T cells. A negative control IgG
antibody and positive control antibody targeting a different
JUN/c-Jun epitope (Bethyl Laboratories) were also used to
demonstrate the specificity of the IP. For blotting
immunoprecipitates, EpiCypher JUN/c-Jun antibody was used at
a dilution of 1:200.
Technical Information
Recommended Dilution
Gene & Protein Information
References
Documents & Resources
Additional Info
This antibody is provided for commercial sale under license from Bethyl Laboratories, Inc.