YEATS2 YEATS Domain, Recombinant Human

SKU: 15-0073
Pack Size: 100 µg
  • Type:  YEATS
  • Mol. Wgt.:  18.9 kDa
  • Expressed In :  E. coli
  • Epitope Tag:  His


YEATS2 YEATS Domain (accession Q9ULM3, amino acids 200-345) expressed in E. coli containing a C-terminal 6xHis tag. The YEATS2 protein is part of the ATAC complex, which has histone acetyltransferase activity on histones H3 and H4 [1]. YEATS2 YEATS Domain recognizes and binds H3K27cr [2].

Validation Data

Figure 1: Protein gel data
YEATS2 YEATS Domain (1 µg) was resolved via PAGE gel and stained with Coomassie blue to demonstrate the size and purity of the protein. The migration and molecular weight of the protein standards are indicated.

Figure 2: Protein interaction data
YEATS2 YEATS Domain demonstrates binding to H3K27cr peptide and no significant binding to H3(15-31) control peptide when analyzed by AlphaScreen® (PerkinElmer). All peptides assayed at 100 nM.

Technical Information

Recombinant 6xHis-tagged protein in 50mM HEPES pH 7.5, 500mM NaCl, 10% glycerol, 2mM TCEP.
Storage and Stability
Stable for six months at -80°C from date of receipt. For best results, aliquot and avoid multiple freeze/thaws.

Application Notes

YEATS2 YEATS Domain is useful for protein binding and screening experiments examining crotonylated protein substrates.


Background References:
[1] Wang et al. J Biol Chem (2008). PMID: 18838386
[2] Zhao et al Cell Res (2016). PMID: 27103431

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