AF9

AF9 YEATS Domain, Recombinant Human

$325.00
SKU: 15-0071
Pack Size: 100 μg
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Type:  YEATS Host:  E. coli
Mol Wgt.:  19.6 kDa Epitope Tag:  His

AF9 YEATS Domain, Recombinant Human Description: Recombinant human AF9 (MLLT3, YEATS3, accession P42568, amino acids 1 to 149) containing an N-terminal 6xHis tag, expressed in E. coli. The AF9 protein is part of the super elongation complex and also associates with the histone methyltransferase DOT1L. Translocation of the AF9 gene to the MLL gene, resulting in an MLL/AF9 fusion is the most common chromosomal rearrangement involving MLL in de novo AML. The YEATS domain of AF9 binds strongly to histone H3K9 acetylation and, to a lesser extent, H3K27 and H3K18 acetylation.

AF9 YEATS Domain, Recombinant Human Formulation: Recombinant 6xHis-tagged protein at 1 mg/ml in 50 mM Tris-HCl pH 7.5, 200 mM NaCl, 1mM DTT, 20% glycerol.
AF9 YEATS Domain, Recombinant Human Storage and Stability: Stable for six months at -80°C from date of receipt. For best results, aliquot and avoid multiple freeze/thaws.
AF9 YEATS Domain, Recombinant Human Application Notes: AF9 YEATS Domain, Recombinant Human is useful for protein binding and screening experiments examining acetylated protein substrates.

References Using this Product:

View technical datasheet for this product. 15-0071 Datasheet
15-0071 Protein Gel Data

Protein Gel Data: AF9 YEATS Domain, Recombinant Human (1 µg) was run on a PAGE gel and stained with Coomassie blue. The migration and molecular weight of the protein standards are indicated.
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